Journal: IEEE sensors journal
Article Title: An Automated Microfluidic Assay for Photonic Crystal Enhanced Detection and Analysis of an Antiviral Antibody Cancer Biomarker in Serum
Figure Lengend Snippet: (a) Schematic of protein microarray printed on the 2 mm × 8 mm PC surface. (b) Bright-field microscope image of protein microarray before washing unbound proteins, where the red region corresponds to the area scanned with the line scanner, with the vertical laser line moving in the direction of the red arrow. (c) Schematic of fluorescence-linked immunosorbent assay (FLISA). ① Human serum was manually pipetted into the microfluidic cartridge and incubated for three hours. ② Phosphate buffered saline with Tween 20 (PBST) was flowed in for 30 s at 30 mBar. ③ Alexa Fluor 647-conjugated secondary antibody was incubated for 1 hour. ④ PBST was followed by drying with N 2 gas. (d) Fluorescence intensity image obtained using the PCEF laser line scanner. (e) Result of the automated spot-finding algorithm. Protein columns for (b, d, e) are as follows: ① AF-SA, ② – ④ Protein A/G, ⑤ cE2 protein, ⑥ GST, ⑦ E7 protein, ⑧ BSA, and ⑨ representative background. Columns 5 and 6 were not evaluated here.
Article Snippet: To wash away unbound material from the serum sample, PBS-Tween 20 (PBST, Sigma-Aldrich) was flowed for 30 s at 30 mBar, followed by N2 gas for 10 s at 50 mBar, and flow-through of secondary antibody tagged with Alexa Fluor 647 (Jackson ImmunoResearch) for 20 s at 30 mBar.
Techniques: Microarray, Microscopy, Fluorescence, Fluorophore-linked Immunoabsorbent Assay, Incubation