tween 20 New England Biolabs Search Results


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  • 99
    New England Biolabs datp
    Datp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2787 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore bsa
    Bsa, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 42788 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs buffer i
    Buffer I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bst dna polymerase
    Bst Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1634 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bst dna polymerase large fragment
    Bst Dna Polymerase Large Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs isothermal buffer
    Isothermal Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs klenow dna polymerase
    Klenow Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs neb buffer 2
    Neb Buffer 2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1495 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs 1x nebuffer 2
    1x Nebuffer 2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bsa molecular biology grade
    Bsa Molecular Biology Grade, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs rnase inhibitor
    Rnase Inhibitor, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bst 2 0 dna polymerase
    Sensitivity of the L . loa RF4-based LAMP assay. Dilutions of genomic L . loa <t>DNA</t> were amplified with the RF4 primer set and Bst 2.0 DNA polymerase in the absence (blue) or presence (red) of the V/DEF additive. Two ul of each dilution was added to LAMP reactions. The average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the concentration of L . loa DNA (ng/ml). All reactions were performed in triplicate. Error bars represent the standard deviation at each point.
    Bst 2 0 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 353 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs buffer d
    Sensitivity of the L . loa RF4-based LAMP assay. Dilutions of genomic L . loa <t>DNA</t> were amplified with the RF4 primer set and Bst 2.0 DNA polymerase in the absence (blue) or presence (red) of the V/DEF additive. Two ul of each dilution was added to LAMP reactions. The average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the concentration of L . loa DNA (ng/ml). All reactions were performed in triplicate. Error bars represent the standard deviation at each point.
    Buffer D, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs exonuclease i e coli
    Sensitivity of the L . loa RF4-based LAMP assay. Dilutions of genomic L . loa <t>DNA</t> were amplified with the RF4 primer set and Bst 2.0 DNA polymerase in the absence (blue) or presence (red) of the V/DEF additive. Two ul of each dilution was added to LAMP reactions. The average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the concentration of L . loa DNA (ng/ml). All reactions were performed in triplicate. Error bars represent the standard deviation at each point.
    Exonuclease I E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 494 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs klenow fragment 3 5 exo
    Sensitivity of the L . loa RF4-based LAMP assay. Dilutions of genomic L . loa <t>DNA</t> were amplified with the RF4 primer set and Bst 2.0 DNA polymerase in the absence (blue) or presence (red) of the V/DEF additive. Two ul of each dilution was added to LAMP reactions. The average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the concentration of L . loa DNA (ng/ml). All reactions were performed in triplicate. Error bars represent the standard deviation at each point.
    Klenow Fragment 3 5 Exo, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1238 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs polynucleotide kinase pnk buffer
    Sensitivity of the L . loa RF4-based LAMP assay. Dilutions of genomic L . loa <t>DNA</t> were amplified with the RF4 primer set and Bst 2.0 DNA polymerase in the absence (blue) or presence (red) of the V/DEF additive. Two ul of each dilution was added to LAMP reactions. The average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the concentration of L . loa DNA (ng/ml). All reactions were performed in triplicate. Error bars represent the standard deviation at each point.
    Polynucleotide Kinase Pnk Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Cell Signaling Technology Inc gli1
    NSCLC cells do not respond to exogenous Shh but can secrete Shh ligand. NSCLC cells were treated or not with recombinant Shh (500 ng/ml) at the indicated times. RT-qPCR was performed to evaluate <t>Gli1</t> and Ptch1 mRNA levels upon treatment in A549 cells ( A ) and H520 cells ( C ). Results are presented as fold differences of mRNA levels (2 ∧∧ Ct) compared with non-treated cells for each time point. Western blot was performed to evaluate Gli1 and Ptch1 protein levels in A549 cells ( B ) and in H520 cells ( D ) treated or not with Shh. ß-actin was used as a loading control. Secretion of human Shh was evaluated in the supernatants of A549 and H520 cells by ELISA ( E ) and confirmed by western blot using an antibody recognizing the secreted active form of Shh ( inset E ). The western blot was performed with H520 supernatant (H520 sup.) and with recombinant Shh (Rec. Shh) used as a positive control. (F ) The knockdown of Shh gene by siRNA was realized in H520 cells. Shh secretion was evaluated by ELISA and expressed in percentage as relative secretion compared with cells transfected with a negative control siRNA (NC) having no homology in vertebrate transcriptome. **p
    Gli1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 859 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs oligo d t20
    NSCLC cells do not respond to exogenous Shh but can secrete Shh ligand. NSCLC cells were treated or not with recombinant Shh (500 ng/ml) at the indicated times. RT-qPCR was performed to evaluate <t>Gli1</t> and Ptch1 mRNA levels upon treatment in A549 cells ( A ) and H520 cells ( C ). Results are presented as fold differences of mRNA levels (2 ∧∧ Ct) compared with non-treated cells for each time point. Western blot was performed to evaluate Gli1 and Ptch1 protein levels in A549 cells ( B ) and in H520 cells ( D ) treated or not with Shh. ß-actin was used as a loading control. Secretion of human Shh was evaluated in the supernatants of A549 and H520 cells by ELISA ( E ) and confirmed by western blot using an antibody recognizing the secreted active form of Shh ( inset E ). The western blot was performed with H520 supernatant (H520 sup.) and with recombinant Shh (Rec. Shh) used as a positive control. (F ) The knockdown of Shh gene by siRNA was realized in H520 cells. Shh secretion was evaluated by ELISA and expressed in percentage as relative secretion compared with cells transfected with a negative control siRNA (NC) having no homology in vertebrate transcriptome. **p
    Oligo D T20, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs standard taq buffer
    NSCLC cells do not respond to exogenous Shh but can secrete Shh ligand. NSCLC cells were treated or not with recombinant Shh (500 ng/ml) at the indicated times. RT-qPCR was performed to evaluate <t>Gli1</t> and Ptch1 mRNA levels upon treatment in A549 cells ( A ) and H520 cells ( C ). Results are presented as fold differences of mRNA levels (2 ∧∧ Ct) compared with non-treated cells for each time point. Western blot was performed to evaluate Gli1 and Ptch1 protein levels in A549 cells ( B ) and in H520 cells ( D ) treated or not with Shh. ß-actin was used as a loading control. Secretion of human Shh was evaluated in the supernatants of A549 and H520 cells by ELISA ( E ) and confirmed by western blot using an antibody recognizing the secreted active form of Shh ( inset E ). The western blot was performed with H520 supernatant (H520 sup.) and with recombinant Shh (Rec. Shh) used as a positive control. (F ) The knockdown of Shh gene by siRNA was realized in H520 cells. Shh secretion was evaluated by ELISA and expressed in percentage as relative secretion compared with cells transfected with a negative control siRNA (NC) having no homology in vertebrate transcriptome. **p
    Standard Taq Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 558 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Sensitivity of the L . loa RF4-based LAMP assay. Dilutions of genomic L . loa DNA were amplified with the RF4 primer set and Bst 2.0 DNA polymerase in the absence (blue) or presence (red) of the V/DEF additive. Two ul of each dilution was added to LAMP reactions. The average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the concentration of L . loa DNA (ng/ml). All reactions were performed in triplicate. Error bars represent the standard deviation at each point.

    Journal: PLoS ONE

    Article Title: Genome Filtering for New DNA Biomarkers of Loa loa Infection Suitable for Loop-Mediated Isothermal Amplification

    doi: 10.1371/journal.pone.0139286

    Figure Lengend Snippet: Sensitivity of the L . loa RF4-based LAMP assay. Dilutions of genomic L . loa DNA were amplified with the RF4 primer set and Bst 2.0 DNA polymerase in the absence (blue) or presence (red) of the V/DEF additive. Two ul of each dilution was added to LAMP reactions. The average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the concentration of L . loa DNA (ng/ml). All reactions were performed in triplicate. Error bars represent the standard deviation at each point.

    Article Snippet: LAMP Assays LAMP reactions contained 1.6 μM each of primers FIP and BIP, 0.2 μM each of F3 and B3, 0.4 μM each of LF and FB, 1.4 mM of each dNTP, 20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween-20 and 8 U of Bst 2.0 DNA Polymerase (New England Biolabs) mixed with one of several template DNAs, or 10 mM Tris-HCl (pH 8), 0.1 mM EDTA for non-template controls (NTC), in a total volume of 25 μl.

    Techniques: Lamp Assay, Amplification, Concentration Assay, Standard Deviation

    Detection of L . loa DNA in spiked blood samples. A two-fold dilution series of genomic L . loa DNA was prepared using uninfected human whole blood. NTCs only contained uninfected human whole blood. After DNA isolation, two μl of each dilution (or NTC) was used in LAMP reactions containing the V/DEF additive with Bst 2.0 DNA polymerase. For each experiment, all samples were assayed in triplicate. Average threshold times and standard deviations are plotted against ng DNA/ml of elution buffer.

    Journal: PLoS ONE

    Article Title: Genome Filtering for New DNA Biomarkers of Loa loa Infection Suitable for Loop-Mediated Isothermal Amplification

    doi: 10.1371/journal.pone.0139286

    Figure Lengend Snippet: Detection of L . loa DNA in spiked blood samples. A two-fold dilution series of genomic L . loa DNA was prepared using uninfected human whole blood. NTCs only contained uninfected human whole blood. After DNA isolation, two μl of each dilution (or NTC) was used in LAMP reactions containing the V/DEF additive with Bst 2.0 DNA polymerase. For each experiment, all samples were assayed in triplicate. Average threshold times and standard deviations are plotted against ng DNA/ml of elution buffer.

    Article Snippet: LAMP Assays LAMP reactions contained 1.6 μM each of primers FIP and BIP, 0.2 μM each of F3 and B3, 0.4 μM each of LF and FB, 1.4 mM of each dNTP, 20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween-20 and 8 U of Bst 2.0 DNA Polymerase (New England Biolabs) mixed with one of several template DNAs, or 10 mM Tris-HCl (pH 8), 0.1 mM EDTA for non-template controls (NTC), in a total volume of 25 μl.

    Techniques: DNA Extraction

    NSCLC cells do not respond to exogenous Shh but can secrete Shh ligand. NSCLC cells were treated or not with recombinant Shh (500 ng/ml) at the indicated times. RT-qPCR was performed to evaluate Gli1 and Ptch1 mRNA levels upon treatment in A549 cells ( A ) and H520 cells ( C ). Results are presented as fold differences of mRNA levels (2 ∧∧ Ct) compared with non-treated cells for each time point. Western blot was performed to evaluate Gli1 and Ptch1 protein levels in A549 cells ( B ) and in H520 cells ( D ) treated or not with Shh. ß-actin was used as a loading control. Secretion of human Shh was evaluated in the supernatants of A549 and H520 cells by ELISA ( E ) and confirmed by western blot using an antibody recognizing the secreted active form of Shh ( inset E ). The western blot was performed with H520 supernatant (H520 sup.) and with recombinant Shh (Rec. Shh) used as a positive control. (F ) The knockdown of Shh gene by siRNA was realized in H520 cells. Shh secretion was evaluated by ELISA and expressed in percentage as relative secretion compared with cells transfected with a negative control siRNA (NC) having no homology in vertebrate transcriptome. **p

    Journal: PLoS ONE

    Article Title: Gli1 Mediates Lung Cancer Cell Proliferation and Sonic Hedgehog-Dependent Mesenchymal Cell Activation

    doi: 10.1371/journal.pone.0063226

    Figure Lengend Snippet: NSCLC cells do not respond to exogenous Shh but can secrete Shh ligand. NSCLC cells were treated or not with recombinant Shh (500 ng/ml) at the indicated times. RT-qPCR was performed to evaluate Gli1 and Ptch1 mRNA levels upon treatment in A549 cells ( A ) and H520 cells ( C ). Results are presented as fold differences of mRNA levels (2 ∧∧ Ct) compared with non-treated cells for each time point. Western blot was performed to evaluate Gli1 and Ptch1 protein levels in A549 cells ( B ) and in H520 cells ( D ) treated or not with Shh. ß-actin was used as a loading control. Secretion of human Shh was evaluated in the supernatants of A549 and H520 cells by ELISA ( E ) and confirmed by western blot using an antibody recognizing the secreted active form of Shh ( inset E ). The western blot was performed with H520 supernatant (H520 sup.) and with recombinant Shh (Rec. Shh) used as a positive control. (F ) The knockdown of Shh gene by siRNA was realized in H520 cells. Shh secretion was evaluated by ELISA and expressed in percentage as relative secretion compared with cells transfected with a negative control siRNA (NC) having no homology in vertebrate transcriptome. **p

    Article Snippet: Membranes were blocked with TBS containing 5% non-fat milk and 0.1%Tween and incubated with Gli1 (Cell Signaling 2643, New England Biolabs, Frankfurt, Germany), Ptch1 (Abcam ab39266, Cambridge, UK), Shh (Cell signaling 2207), cyclin D2 (Cell signaling 3741) or β-Actin-Peroxidase (Sigma A3854).

    Techniques: Recombinant, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Positive Control, Transfection, Negative Control

    The supernatant of H520 cells activates Hedgehog pathway in lung fibroblasts . Lung fibroblasts were serum-starved for 24 hours and then treated or not with the supernatant of H520 cells for 24, 48 or 72 hours. ( A ) RT-qPCR was performed to analyze Gli1, Gli2, Gli3 and Ptch1 mRNA levels in CCL206 treated with H520 supernatant. Results are presented as fold of RNA levels in treated cells compared with non-treated cells for each time point. **p

    Journal: PLoS ONE

    Article Title: Gli1 Mediates Lung Cancer Cell Proliferation and Sonic Hedgehog-Dependent Mesenchymal Cell Activation

    doi: 10.1371/journal.pone.0063226

    Figure Lengend Snippet: The supernatant of H520 cells activates Hedgehog pathway in lung fibroblasts . Lung fibroblasts were serum-starved for 24 hours and then treated or not with the supernatant of H520 cells for 24, 48 or 72 hours. ( A ) RT-qPCR was performed to analyze Gli1, Gli2, Gli3 and Ptch1 mRNA levels in CCL206 treated with H520 supernatant. Results are presented as fold of RNA levels in treated cells compared with non-treated cells for each time point. **p

    Article Snippet: Membranes were blocked with TBS containing 5% non-fat milk and 0.1%Tween and incubated with Gli1 (Cell Signaling 2643, New England Biolabs, Frankfurt, Germany), Ptch1 (Abcam ab39266, Cambridge, UK), Shh (Cell signaling 2207), cyclin D2 (Cell signaling 3741) or β-Actin-Peroxidase (Sigma A3854).

    Techniques: Quantitative RT-PCR

    Lung fibroblasts respond to Shh treatment. Mouse lung fibroblasts CCL206 were treated or not with 500 ng/ml of recombinant mouse Shh or 500 ng/ml of human Shh for 24, 48 and 72 hours. ( A ) mRNA levels of Gli1, Gli2, Gli3 and Ptch1 upon treatment were assessed by RT-qPCR. Results are presented as fold of differences in mRNA levels (2 ∧∧ Ct) of treated cells compared with non-treated cells for each time point. *p

    Journal: PLoS ONE

    Article Title: Gli1 Mediates Lung Cancer Cell Proliferation and Sonic Hedgehog-Dependent Mesenchymal Cell Activation

    doi: 10.1371/journal.pone.0063226

    Figure Lengend Snippet: Lung fibroblasts respond to Shh treatment. Mouse lung fibroblasts CCL206 were treated or not with 500 ng/ml of recombinant mouse Shh or 500 ng/ml of human Shh for 24, 48 and 72 hours. ( A ) mRNA levels of Gli1, Gli2, Gli3 and Ptch1 upon treatment were assessed by RT-qPCR. Results are presented as fold of differences in mRNA levels (2 ∧∧ Ct) of treated cells compared with non-treated cells for each time point. *p

    Article Snippet: Membranes were blocked with TBS containing 5% non-fat milk and 0.1%Tween and incubated with Gli1 (Cell Signaling 2643, New England Biolabs, Frankfurt, Germany), Ptch1 (Abcam ab39266, Cambridge, UK), Shh (Cell signaling 2207), cyclin D2 (Cell signaling 3741) or β-Actin-Peroxidase (Sigma A3854).

    Techniques: Recombinant, Quantitative RT-PCR