tween 20 New England Biolabs Search Results


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  • 95
    New England Biolabs datp
    Datp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1853 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore bsa
    Bsa, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 42788 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bst dna polymerase
    Bst Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bst dna polymerase large fragment
    Bst Dna Polymerase Large Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs isothermal buffer
    Isothermal Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs klenow exo enzyme reaction mix
    Klenow Exo Enzyme Reaction Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs bst 2 0 dna polymerase
    Sensitivity of the L . loa RF4-based LAMP assay. Dilutions of genomic L . loa <t>DNA</t> were amplified with the RF4 primer set and Bst 2.0 DNA polymerase in the absence (blue) or presence (red) of the V/DEF additive. Two ul of each dilution was added to LAMP reactions. The average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the concentration of L . loa DNA (ng/ml). All reactions were performed in triplicate. Error bars represent the standard deviation at each point.
    Bst 2 0 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    New England Biolabs vanadyl ribonucleoside
    Sensitivity of the L . loa RF4-based LAMP assay. Dilutions of genomic L . loa <t>DNA</t> were amplified with the RF4 primer set and Bst 2.0 DNA polymerase in the absence (blue) or presence (red) of the V/DEF additive. Two ul of each dilution was added to LAMP reactions. The average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the concentration of L . loa DNA (ng/ml). All reactions were performed in triplicate. Error bars represent the standard deviation at each point.
    Vanadyl Ribonucleoside, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs iκbα
    Effects of vitamin D 3 on cytokine secretion profile and NFκB activation. Preadipocytes were pretreated with or without vitamin D 3 (10 nM) for 24 h. Cell culture medium was harvested 24 h later, mixed with a cocktail of biotinylated detection antibodies and then incubated with a human cytokine array (panel A, R D). (A) Signals were detected by chemiluminescence. (B) The table shows the location of detected proteins on the membrane. (C) Detected signals were quantified as the pixel density. (D-E) levels of MCP-1 and IL-6 release into cell culture medium, measured by ELISA. Results are expressed as means ± SEM for groups of 6; the results were confirmed by three independent experiments. (F-G) preadipocytes were pre-incubated with vitamin D 3 (10 and 100 nM) for 24 h; protein expression of <t>IκBα</t> in cell lysates was analyzed by western blotting (F) and the signals quantified by densitometry (G); the results were confirmed by two independent experiments. ** P
    Iκbα, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs neb buffer 2
    Effects of vitamin D 3 on cytokine secretion profile and NFκB activation. Preadipocytes were pretreated with or without vitamin D 3 (10 nM) for 24 h. Cell culture medium was harvested 24 h later, mixed with a cocktail of biotinylated detection antibodies and then incubated with a human cytokine array (panel A, R D). (A) Signals were detected by chemiluminescence. (B) The table shows the location of detected proteins on the membrane. (C) Detected signals were quantified as the pixel density. (D-E) levels of MCP-1 and IL-6 release into cell culture medium, measured by ELISA. Results are expressed as means ± SEM for groups of 6; the results were confirmed by three independent experiments. (F-G) preadipocytes were pre-incubated with vitamin D 3 (10 and 100 nM) for 24 h; protein expression of <t>IκBα</t> in cell lysates was analyzed by western blotting (F) and the signals quantified by densitometry (G); the results were confirmed by two independent experiments. ** P
    Neb Buffer 2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    New England Biolabs tween20
    Effects of vitamin D 3 on cytokine secretion profile and NFκB activation. Preadipocytes were pretreated with or without vitamin D 3 (10 nM) for 24 h. Cell culture medium was harvested 24 h later, mixed with a cocktail of biotinylated detection antibodies and then incubated with a human cytokine array (panel A, R D). (A) Signals were detected by chemiluminescence. (B) The table shows the location of detected proteins on the membrane. (C) Detected signals were quantified as the pixel density. (D-E) levels of MCP-1 and IL-6 release into cell culture medium, measured by ELISA. Results are expressed as means ± SEM for groups of 6; the results were confirmed by three independent experiments. (F-G) preadipocytes were pre-incubated with vitamin D 3 (10 and 100 nM) for 24 h; protein expression of <t>IκBα</t> in cell lysates was analyzed by western blotting (F) and the signals quantified by densitometry (G); the results were confirmed by two independent experiments. ** P
    Tween20, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs bst 2 0 warmstart dna polymerase
    Effects of vitamin D 3 on cytokine secretion profile and NFκB activation. Preadipocytes were pretreated with or without vitamin D 3 (10 nM) for 24 h. Cell culture medium was harvested 24 h later, mixed with a cocktail of biotinylated detection antibodies and then incubated with a human cytokine array (panel A, R D). (A) Signals were detected by chemiluminescence. (B) The table shows the location of detected proteins on the membrane. (C) Detected signals were quantified as the pixel density. (D-E) levels of MCP-1 and IL-6 release into cell culture medium, measured by ELISA. Results are expressed as means ± SEM for groups of 6; the results were confirmed by three independent experiments. (F-G) preadipocytes were pre-incubated with vitamin D 3 (10 and 100 nM) for 24 h; protein expression of <t>IκBα</t> in cell lysates was analyzed by western blotting (F) and the signals quantified by densitometry (G); the results were confirmed by two independent experiments. ** P
    Bst 2 0 Warmstart Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 275 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    New England Biolabs anti rabbit secondary antibody
    Effects of vitamin D 3 on cytokine secretion profile and NFκB activation. Preadipocytes were pretreated with or without vitamin D 3 (10 nM) for 24 h. Cell culture medium was harvested 24 h later, mixed with a cocktail of biotinylated detection antibodies and then incubated with a human cytokine array (panel A, R D). (A) Signals were detected by chemiluminescence. (B) The table shows the location of detected proteins on the membrane. (C) Detected signals were quantified as the pixel density. (D-E) levels of MCP-1 and IL-6 release into cell culture medium, measured by ELISA. Results are expressed as means ± SEM for groups of 6; the results were confirmed by three independent experiments. (F-G) preadipocytes were pre-incubated with vitamin D 3 (10 and 100 nM) for 24 h; protein expression of <t>IκBα</t> in cell lysates was analyzed by western blotting (F) and the signals quantified by densitometry (G); the results were confirmed by two independent experiments. ** P
    Anti Rabbit Secondary Antibody, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs bsa molecular biology grade
    Effects of vitamin D 3 on cytokine secretion profile and NFκB activation. Preadipocytes were pretreated with or without vitamin D 3 (10 nM) for 24 h. Cell culture medium was harvested 24 h later, mixed with a cocktail of biotinylated detection antibodies and then incubated with a human cytokine array (panel A, R D). (A) Signals were detected by chemiluminescence. (B) The table shows the location of detected proteins on the membrane. (C) Detected signals were quantified as the pixel density. (D-E) levels of MCP-1 and IL-6 release into cell culture medium, measured by ELISA. Results are expressed as means ± SEM for groups of 6; the results were confirmed by three independent experiments. (F-G) preadipocytes were pre-incubated with vitamin D 3 (10 and 100 nM) for 24 h; protein expression of <t>IκBα</t> in cell lysates was analyzed by western blotting (F) and the signals quantified by densitometry (G); the results were confirmed by two independent experiments. ** P
    Bsa Molecular Biology Grade, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    New England Biolabs tween20 proteinase k
    Effects of vitamin D 3 on cytokine secretion profile and NFκB activation. Preadipocytes were pretreated with or without vitamin D 3 (10 nM) for 24 h. Cell culture medium was harvested 24 h later, mixed with a cocktail of biotinylated detection antibodies and then incubated with a human cytokine array (panel A, R D). (A) Signals were detected by chemiluminescence. (B) The table shows the location of detected proteins on the membrane. (C) Detected signals were quantified as the pixel density. (D-E) levels of MCP-1 and IL-6 release into cell culture medium, measured by ELISA. Results are expressed as means ± SEM for groups of 6; the results were confirmed by three independent experiments. (F-G) preadipocytes were pre-incubated with vitamin D 3 (10 and 100 nM) for 24 h; protein expression of <t>IκBα</t> in cell lysates was analyzed by western blotting (F) and the signals quantified by densitometry (G); the results were confirmed by two independent experiments. ** P
    Tween20 Proteinase K, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Sensitivity of the L . loa RF4-based LAMP assay. Dilutions of genomic L . loa DNA were amplified with the RF4 primer set and Bst 2.0 DNA polymerase in the absence (blue) or presence (red) of the V/DEF additive. Two ul of each dilution was added to LAMP reactions. The average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the concentration of L . loa DNA (ng/ml). All reactions were performed in triplicate. Error bars represent the standard deviation at each point.

    Journal: PLoS ONE

    Article Title: Genome Filtering for New DNA Biomarkers of Loa loa Infection Suitable for Loop-Mediated Isothermal Amplification

    doi: 10.1371/journal.pone.0139286

    Figure Lengend Snippet: Sensitivity of the L . loa RF4-based LAMP assay. Dilutions of genomic L . loa DNA were amplified with the RF4 primer set and Bst 2.0 DNA polymerase in the absence (blue) or presence (red) of the V/DEF additive. Two ul of each dilution was added to LAMP reactions. The average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the concentration of L . loa DNA (ng/ml). All reactions were performed in triplicate. Error bars represent the standard deviation at each point.

    Article Snippet: LAMP Assays LAMP reactions contained 1.6 μM each of primers FIP and BIP, 0.2 μM each of F3 and B3, 0.4 μM each of LF and FB, 1.4 mM of each dNTP, 20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween-20 and 8 U of Bst 2.0 DNA Polymerase (New England Biolabs) mixed with one of several template DNAs, or 10 mM Tris-HCl (pH 8), 0.1 mM EDTA for non-template controls (NTC), in a total volume of 25 μl.

    Techniques: Lamp Assay, Amplification, Concentration Assay, Standard Deviation

    Detection of L . loa DNA in spiked blood samples. A two-fold dilution series of genomic L . loa DNA was prepared using uninfected human whole blood. NTCs only contained uninfected human whole blood. After DNA isolation, two μl of each dilution (or NTC) was used in LAMP reactions containing the V/DEF additive with Bst 2.0 DNA polymerase. For each experiment, all samples were assayed in triplicate. Average threshold times and standard deviations are plotted against ng DNA/ml of elution buffer.

    Journal: PLoS ONE

    Article Title: Genome Filtering for New DNA Biomarkers of Loa loa Infection Suitable for Loop-Mediated Isothermal Amplification

    doi: 10.1371/journal.pone.0139286

    Figure Lengend Snippet: Detection of L . loa DNA in spiked blood samples. A two-fold dilution series of genomic L . loa DNA was prepared using uninfected human whole blood. NTCs only contained uninfected human whole blood. After DNA isolation, two μl of each dilution (or NTC) was used in LAMP reactions containing the V/DEF additive with Bst 2.0 DNA polymerase. For each experiment, all samples were assayed in triplicate. Average threshold times and standard deviations are plotted against ng DNA/ml of elution buffer.

    Article Snippet: LAMP Assays LAMP reactions contained 1.6 μM each of primers FIP and BIP, 0.2 μM each of F3 and B3, 0.4 μM each of LF and FB, 1.4 mM of each dNTP, 20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween-20 and 8 U of Bst 2.0 DNA Polymerase (New England Biolabs) mixed with one of several template DNAs, or 10 mM Tris-HCl (pH 8), 0.1 mM EDTA for non-template controls (NTC), in a total volume of 25 μl.

    Techniques: DNA Extraction

    Effects of vitamin D 3 on cytokine secretion profile and NFκB activation. Preadipocytes were pretreated with or without vitamin D 3 (10 nM) for 24 h. Cell culture medium was harvested 24 h later, mixed with a cocktail of biotinylated detection antibodies and then incubated with a human cytokine array (panel A, R D). (A) Signals were detected by chemiluminescence. (B) The table shows the location of detected proteins on the membrane. (C) Detected signals were quantified as the pixel density. (D-E) levels of MCP-1 and IL-6 release into cell culture medium, measured by ELISA. Results are expressed as means ± SEM for groups of 6; the results were confirmed by three independent experiments. (F-G) preadipocytes were pre-incubated with vitamin D 3 (10 and 100 nM) for 24 h; protein expression of IκBα in cell lysates was analyzed by western blotting (F) and the signals quantified by densitometry (G); the results were confirmed by two independent experiments. ** P

    Journal: International journal of obesity (2005)

    Article Title: 1,25-dihydroxyvitamin D3 inhibits the cytokine-induced secretion of MCP-1 and reduces monocyte recruitment by human preadipocytes

    doi: 10.1038/ijo.2012.53

    Figure Lengend Snippet: Effects of vitamin D 3 on cytokine secretion profile and NFκB activation. Preadipocytes were pretreated with or without vitamin D 3 (10 nM) for 24 h. Cell culture medium was harvested 24 h later, mixed with a cocktail of biotinylated detection antibodies and then incubated with a human cytokine array (panel A, R D). (A) Signals were detected by chemiluminescence. (B) The table shows the location of detected proteins on the membrane. (C) Detected signals were quantified as the pixel density. (D-E) levels of MCP-1 and IL-6 release into cell culture medium, measured by ELISA. Results are expressed as means ± SEM for groups of 6; the results were confirmed by three independent experiments. (F-G) preadipocytes were pre-incubated with vitamin D 3 (10 and 100 nM) for 24 h; protein expression of IκBα in cell lysates was analyzed by western blotting (F) and the signals quantified by densitometry (G); the results were confirmed by two independent experiments. ** P

    Article Snippet: For immunodetection, the membrane was blocked for 1 h at room temperature with Tris-buffered saline (TBS) containing 0.1% Tween 20 and 5% non-fat milk and incubated overnight at 4°C with an antibody for IκBα (New England BioLabs Ltd, Hitchin, UK) at a 1:1000 dilution in 5% non-fat milk TBS and 0.1% Tween-20 followed by an anti-mouse secondary antibody (Bio-Rad) at 1:2000 dilution.

    Techniques: Activation Assay, Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot