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  • 99
    Thermo Fisher turbofect transfection reagent
    Figure 2. ABCC1-mediated GSH efflux modulated autophagy in HeLa cells. ( A ) HeLa cells were transfected with either a nontargeting <t>siRNA</t> (siScr) or an siRNA targeting ABCC1 (si ABCC1 ). Twelve hours after <t>transfection</t> cells were starved with HBSS
    Turbofect Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/turbofect transfection reagent/product/Thermo Fisher
    Average 99 stars, based on 29398 article reviews
    Price from $9.99 to $1999.99
    turbofect transfection reagent - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher turbofect in vitro transfection reagent
    Figure 2. ABCC1-mediated GSH efflux modulated autophagy in HeLa cells. ( A ) HeLa cells were transfected with either a nontargeting <t>siRNA</t> (siScr) or an siRNA targeting ABCC1 (si ABCC1 ). Twelve hours after <t>transfection</t> cells were starved with HBSS
    Turbofect In Vitro Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2406 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/turbofect in vitro transfection reagent/product/Thermo Fisher
    Average 99 stars, based on 2406 article reviews
    Price from $9.99 to $1999.99
    turbofect in vitro transfection reagent - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    Fisher Scientific turbofect transfection reagent
    Figure 2. ABCC1-mediated GSH efflux modulated autophagy in HeLa cells. ( A ) HeLa cells were transfected with either a nontargeting <t>siRNA</t> (siScr) or an siRNA targeting ABCC1 (si ABCC1 ). Twelve hours after <t>transfection</t> cells were starved with HBSS
    Turbofect Transfection Reagent, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/turbofect transfection reagent/product/Fisher Scientific
    Average 92 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    turbofect transfection reagent - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Figure 2. ABCC1-mediated GSH efflux modulated autophagy in HeLa cells. ( A ) HeLa cells were transfected with either a nontargeting siRNA (siScr) or an siRNA targeting ABCC1 (si ABCC1 ). Twelve hours after transfection cells were starved with HBSS

    Journal: Autophagy

    Article Title: Glutathione participates in the modulation of starvation-induced autophagy in carcinoma cells

    doi: 10.4161/auto.22037

    Figure Lengend Snippet: Figure 2. ABCC1-mediated GSH efflux modulated autophagy in HeLa cells. ( A ) HeLa cells were transfected with either a nontargeting siRNA (siScr) or an siRNA targeting ABCC1 (si ABCC1 ). Twelve hours after transfection cells were starved with HBSS

    Article Snippet: TurboFect™ in vitro Transfection Reagent (R0534) and TurboFect siRNA Transfection Reagent (R1401) were from Thermo Scientific.

    Techniques: Transfection

    Figure 3. ABCC1-mediated GSH efflux modulated autophagy in HepG2 and H1299 cells. ( A ) HepG2 and H1299 cells were transfected with either a nontargeting siRNA (siScr) or an siRNA targeting ABCC1 (si ABCC1 ). Twelve hours after transfection cells

    Journal: Autophagy

    Article Title: Glutathione participates in the modulation of starvation-induced autophagy in carcinoma cells

    doi: 10.4161/auto.22037

    Figure Lengend Snippet: Figure 3. ABCC1-mediated GSH efflux modulated autophagy in HepG2 and H1299 cells. ( A ) HepG2 and H1299 cells were transfected with either a nontargeting siRNA (siScr) or an siRNA targeting ABCC1 (si ABCC1 ). Twelve hours after transfection cells

    Article Snippet: TurboFect™ in vitro Transfection Reagent (R0534) and TurboFect siRNA Transfection Reagent (R1401) were from Thermo Scientific.

    Techniques: Transfection

    Inhibition and activation of Notch signaling, respectively, in both MCF-7 and MDA-MB-231 cells. Normal group: MCF-7 or MDA-MB-231 cells were incubated under normal conditions; shNC group: MCF-7 or MDA-MB-231 cells were stably transfected with NC shRNA; shNotch1 group: MCF-7 or MDA-MB-231 cells were stably transfected with Notch1 shRNA; normal + Jagged1: MCF-7 or MDA-MB-231 cells were treated with Jagged1 for 48 h; shNC + Jagged1 group: MCF-7 or MDA-MB-231 cells were treated with Jagged1 for 48 h after stable shNC transfection. Jagged1 + shNotch1 group: MCF-7 or MDA-MB-231 cells were treated with Jagged1 for 48 h and then Notch1 shRNA was transiently transfected for an additional 48 h. (A) , (C) , and (E) Total protein was isolated for western blot analysis using Notch1, Hey1, Hes1, and NF-κB65 antibodies. β-Actin was used as a loading control. (B) , (D) , and (F) Total RNA was extracted, and the expression levels of Notch1, Hey1, Hes1, and NF-κB65 were assayed by real-time PCR. The expression of every target gene was quantified using GAPDH as a normalization control. The data are from three independent experiments. Column: mean; bar: SD. The symbol * represents a significant difference (P

    Journal: Molecular Cancer

    Article Title: Notch1 signaling regulates the epithelial–mesenchymal transition and invasion of breast cancer in a Slug-dependent manner

    doi: 10.1186/s12943-015-0295-3

    Figure Lengend Snippet: Inhibition and activation of Notch signaling, respectively, in both MCF-7 and MDA-MB-231 cells. Normal group: MCF-7 or MDA-MB-231 cells were incubated under normal conditions; shNC group: MCF-7 or MDA-MB-231 cells were stably transfected with NC shRNA; shNotch1 group: MCF-7 or MDA-MB-231 cells were stably transfected with Notch1 shRNA; normal + Jagged1: MCF-7 or MDA-MB-231 cells were treated with Jagged1 for 48 h; shNC + Jagged1 group: MCF-7 or MDA-MB-231 cells were treated with Jagged1 for 48 h after stable shNC transfection. Jagged1 + shNotch1 group: MCF-7 or MDA-MB-231 cells were treated with Jagged1 for 48 h and then Notch1 shRNA was transiently transfected for an additional 48 h. (A) , (C) , and (E) Total protein was isolated for western blot analysis using Notch1, Hey1, Hes1, and NF-κB65 antibodies. β-Actin was used as a loading control. (B) , (D) , and (F) Total RNA was extracted, and the expression levels of Notch1, Hey1, Hes1, and NF-κB65 were assayed by real-time PCR. The expression of every target gene was quantified using GAPDH as a normalization control. The data are from three independent experiments. Column: mean; bar: SD. The symbol * represents a significant difference (P

    Article Snippet: Cells (5 × 104 per well) were seeded in six-well plates 24 h prior to transfection and transfected with 80 pM siRNA using TurboFectTM siRNA Transfection Reagent (Fermentas, Beijing, China), according to the manufacturer’s protocol.

    Techniques: Inhibition, Activation Assay, Multiple Displacement Amplification, Incubation, Stable Transfection, Transfection, shRNA, Isolation, Western Blot, Expressing, Real-time Polymerase Chain Reaction

    HIF-2α promotes miR-380-5p transcription. A, overexpression of HIF-2α up-regulates miR-380-5p. HCT116 cells were transfected with HA-tagged HIF-1α or HIF-2α. 36 h after transfection the expression of miR-380-5p was analyzed with qRT-PCR and normalized to U6 (mean ± S.D., n = 3, ***, p

    Journal: The Journal of Biological Chemistry

    Article Title: IDH1 Arg-132 mutant promotes tumor formation through down-regulating p53

    doi: 10.1074/jbc.RA117.001385

    Figure Lengend Snippet: HIF-2α promotes miR-380-5p transcription. A, overexpression of HIF-2α up-regulates miR-380-5p. HCT116 cells were transfected with HA-tagged HIF-1α or HIF-2α. 36 h after transfection the expression of miR-380-5p was analyzed with qRT-PCR and normalized to U6 (mean ± S.D., n = 3, ***, p

    Article Snippet: Lentivirus for infection of MEF and HCT116 cells was packaged in HEK293T cells using Turbofect (catalog number R0532, Thermo Scientific) transfection reagent.

    Techniques: Over Expression, Transfection, Expressing, Quantitative RT-PCR