turbo dnase Search Results


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  • 99
    Thermo Fisher turbo dnase
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbo Dnase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22364 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher turbos dnase
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbos Dnase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific turbo dnase
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbo Dnase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 97/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Bio-Rad turbo dnase
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbo Dnase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Roche turbo dnase
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbo Dnase, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    InvivoGen turbo dnase kit
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbo Dnase Kit, supplied by InvivoGen, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher turbo dnase step
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbo Dnase Step, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ambion turbo dnase
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Ambion Turbo Dnase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 691 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega dnase turbo dnafree
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Dnase Turbo Dnafree, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher 2701 turbo dnase
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    2701 Turbo Dnase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Amgen turbo dnase kit
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbo Dnase Kit, supplied by Amgen, used in various techniques. Bioz Stars score: 84/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific turbo dnase kit
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbo Dnase Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher turbo dnase buffer
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbo Dnase Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher 1x turbo dnase buffer
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    1x Turbo Dnase Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher turbo dnafree dnase
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbo Dnafree Dnase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad turbo dnase digestion
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbo Dnase Digestion, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies turbo dnase
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbo Dnase, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa turbo dnase
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbo Dnase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Biocolor Ltd turbo dnase kit
    <t>DNase</t> and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. <t>TURBO™</t> DNase (DNase) was used as a positive control.
    Turbo Dnase Kit, supplied by Biocolor Ltd, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    DNase and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. TURBO™ DNase (DNase) was used as a positive control.

    Journal: Scientific Reports

    Article Title: Distinct properties of proteases and nucleases in the gut, salivary gland and saliva of southern green stink bug, Nezara viridula

    doi: 10.1038/srep27587

    Figure Lengend Snippet: DNase and RNase activities and degradation of DNA by Nezara viridula nucleases. ( a ) Total and specific nuclease activities in gut, salivary gland and saliva (letters indicate significantly different groups; P ≤ 0.05; Student’s t -test). ( b ) Degradation of DNA by DNases from gut, salivary gland and saliva. Samples (gut, salivary gland or saliva) were incubated with DNA for 5, 10 and 30 min. Samples were then run on 2% agarose gels and DNA visualized by ethidium bromide staining. TURBO™ DNase (DNase) was used as a positive control.

    Article Snippet: Gut, salivary gland extracts and saliva (5 μg protein per sample in a volume of 10 μL) were added to 200 μL DNA or RNA (100 μg/mL) and the reaction was carried out at 37 °C for 30 min. TURBO™ DNase (5 units) (Life Technologies, Carlsbad, CA, USA) and RNase A (20 μg) (Thermo Scientific, Waltham, MA, USA) were used as positive controls.

    Techniques: Incubation, Staining, Positive Control

    OhrR binds specifically to ohr and ohrR promoter DNA. (A) Electropherogram traces of DNase I-digested ohr promoter DNA. (B) Electropherogram traces of DNase I-digested ohrR promoter DNA. Red, DNA only; blue, DNA incubated with OhrR at a stoichiometric DNA/protein ratio of 1:4. The protected regions are expanded in the lower panels, with the sequence being identified at the bottom. Numbering is relative to the translational start, defined as position +1. For ohrR promoter DNA, the ATG codon is underlined. For ohr promoter DNA, a sequence resembling the published OhrR consensus sequence is identified by a dashed underline; the asterisk marks a hypersensitive site. The results are representative of those from at least three replicates.

    Journal: Infection and Immunity

    Article Title: Gene Regulation by Redox-Sensitive Burkholderia thailandensis OhrR and Its Role in Bacterial Killing of Caenorhabditis elegans

    doi: 10.1128/IAI.00322-18

    Figure Lengend Snippet: OhrR binds specifically to ohr and ohrR promoter DNA. (A) Electropherogram traces of DNase I-digested ohr promoter DNA. (B) Electropherogram traces of DNase I-digested ohrR promoter DNA. Red, DNA only; blue, DNA incubated with OhrR at a stoichiometric DNA/protein ratio of 1:4. The protected regions are expanded in the lower panels, with the sequence being identified at the bottom. Numbering is relative to the translational start, defined as position +1. For ohrR promoter DNA, the ATG codon is underlined. For ohr promoter DNA, a sequence resembling the published OhrR consensus sequence is identified by a dashed underline; the asterisk marks a hypersensitive site. The results are representative of those from at least three replicates.

    Article Snippet: DNA contamination was removed using a Turbo DNase kit (Ambion), and the absence of DNA was verified by PCR.

    Techniques: Incubation, Sequencing

    Effect of OhrR oxidation on binding to ohr promoter DNA. (A) Electropherogram traces of DNase I-digested ohr promoter DNA and DNA incubated with reduced OhrR. (B) DNA incubated with reduced OhrR or with OhrR oxidized with 7 μM CHP. (C) DNA incubated with reduced OhrR or with OhrR oxidized with 70 μM CHP. (D) DNA incubated with OhrR oxidized with 70 μM CHP or with 70 μM CHP-oxidized OhrR subsequently rereduced with DTT. (E) DNA incubated with reduced OhrR or with OhrR oxidized with 0.7 mM H 2 O 2 . (F) Free ohr promoter DNA and DNA incubated with OhrR oxidized with 7 μM NaOCl. Asterisks mark hypersensitive sites (positions −56 and −49). The results are representative of those from at least three replicates.

    Journal: Infection and Immunity

    Article Title: Gene Regulation by Redox-Sensitive Burkholderia thailandensis OhrR and Its Role in Bacterial Killing of Caenorhabditis elegans

    doi: 10.1128/IAI.00322-18

    Figure Lengend Snippet: Effect of OhrR oxidation on binding to ohr promoter DNA. (A) Electropherogram traces of DNase I-digested ohr promoter DNA and DNA incubated with reduced OhrR. (B) DNA incubated with reduced OhrR or with OhrR oxidized with 7 μM CHP. (C) DNA incubated with reduced OhrR or with OhrR oxidized with 70 μM CHP. (D) DNA incubated with OhrR oxidized with 70 μM CHP or with 70 μM CHP-oxidized OhrR subsequently rereduced with DTT. (E) DNA incubated with reduced OhrR or with OhrR oxidized with 0.7 mM H 2 O 2 . (F) Free ohr promoter DNA and DNA incubated with OhrR oxidized with 7 μM NaOCl. Asterisks mark hypersensitive sites (positions −56 and −49). The results are representative of those from at least three replicates.

    Article Snippet: DNA contamination was removed using a Turbo DNase kit (Ambion), and the absence of DNA was verified by PCR.

    Techniques: Binding Assay, Incubation

    Dissection of the consensus binding motif of PhoP Xcc . (A) Venn diagram showing the number of PhoP Xcc -binding promoter regions identified by the ChIP-seq analysis. (B) Functional categories of all genes with promoters bound by PhoP Xcc under PhoQ Xcc induction and repression conditions. (C) Consensus PhoP Xcc -binding DNA motifs predicted by ChIP-seq and MEME. The height of each nucleotide is proportional to the level of conservation at that site. Upper and lower panels: motifs predicted under PhoQ Xcc induction and repression conditions, respectively. (D–F) Binding sites of PhoP Xcc on the promoter regions of (D) oar , (E), phoP , and (F) ftsA . DNase I footprinting assays were conducted. The amounts of PhoP Xcc protein used in the reactions were 1: zero; 2: 40 μM, 3: 80 μM, 4: 120 μM, and 5: 160 μM. The DNA regions protected by PhoP Xcc are shown on the right of the footprinting results, with the PhoP Xcc -binding sites shown in red. (G–I) PhoP Xcc –DNA-binding affinities were quantified by MST. (G) PhoP Xcc –P ftsA Xcc . (H) PhoP Xcc –P phoP Xcc . (I) PhoP Xcc –P oar Xcc . DNA probes were labeled with FAM and used for MST. The FAM-labeled DNA (20 nM) was incubated with the PhoP Xcc protein in an NT standard capillary in the MST assay. Titrations of the PhoP Xcc protein ranged from 1.5 to 50 μM. The solid curve is the fit of the data points to the standard KD-Fit function. The black bars represent SD ( n = 3). K d = dissociation constant. ChIP-seq, chromatin immunoprecipitation sequencing; FAM, fluorescein; MST, microscale thermophoresis; NT, NanoTemper.

    Journal: Genetics

    Article Title: An Essential Regulatory System Originating from Polygenic Transcriptional Rewiring of PhoP-PhoQ of Xanthomonas campestris

    doi: 10.1534/genetics.117.200204

    Figure Lengend Snippet: Dissection of the consensus binding motif of PhoP Xcc . (A) Venn diagram showing the number of PhoP Xcc -binding promoter regions identified by the ChIP-seq analysis. (B) Functional categories of all genes with promoters bound by PhoP Xcc under PhoQ Xcc induction and repression conditions. (C) Consensus PhoP Xcc -binding DNA motifs predicted by ChIP-seq and MEME. The height of each nucleotide is proportional to the level of conservation at that site. Upper and lower panels: motifs predicted under PhoQ Xcc induction and repression conditions, respectively. (D–F) Binding sites of PhoP Xcc on the promoter regions of (D) oar , (E), phoP , and (F) ftsA . DNase I footprinting assays were conducted. The amounts of PhoP Xcc protein used in the reactions were 1: zero; 2: 40 μM, 3: 80 μM, 4: 120 μM, and 5: 160 μM. The DNA regions protected by PhoP Xcc are shown on the right of the footprinting results, with the PhoP Xcc -binding sites shown in red. (G–I) PhoP Xcc –DNA-binding affinities were quantified by MST. (G) PhoP Xcc –P ftsA Xcc . (H) PhoP Xcc –P phoP Xcc . (I) PhoP Xcc –P oar Xcc . DNA probes were labeled with FAM and used for MST. The FAM-labeled DNA (20 nM) was incubated with the PhoP Xcc protein in an NT standard capillary in the MST assay. Titrations of the PhoP Xcc protein ranged from 1.5 to 50 μM. The solid curve is the fit of the data points to the standard KD-Fit function. The black bars represent SD ( n = 3). K d = dissociation constant. ChIP-seq, chromatin immunoprecipitation sequencing; FAM, fluorescein; MST, microscale thermophoresis; NT, NanoTemper.

    Article Snippet: One microgram of RNA from each sample was treated with Turbo DNA-free DNase (Ambion) before RT-PCR, to exclude DNA contamination.

    Techniques: Dissection, Binding Assay, Chromatin Immunoprecipitation, Functional Assay, Footprinting, Microscale Thermophoresis, Labeling, Incubation, ChIP-sequencing

    Effect of the K297R mutation on progeny virus release. iSLK-BAC16 and iSLK-BAC-K297R cells were induced with Dox and butyrate for indicated time. The extracellular virions were collected from culture media and treated with Turbo DNase I. Viral DNAs were extracted and KSHV genomic DNA copy numbers were estimated by qPCR along with external standards of known concentrations of the viral DNA with primers against the ORF73 gene (A). Intracellular KSHV genomic DNAs were extracted from harvested cells and quantitated by qPCR as above (B). (*,  p

    Journal: PLoS Pathogens

    Article Title: Mono-ubiquitylated ORF45 Mediates Association of KSHV Particles with Internal Lipid Rafts for Viral Assembly and Egress

    doi: 10.1371/journal.ppat.1005332

    Figure Lengend Snippet: Effect of the K297R mutation on progeny virus release. iSLK-BAC16 and iSLK-BAC-K297R cells were induced with Dox and butyrate for indicated time. The extracellular virions were collected from culture media and treated with Turbo DNase I. Viral DNAs were extracted and KSHV genomic DNA copy numbers were estimated by qPCR along with external standards of known concentrations of the viral DNA with primers against the ORF73 gene (A). Intracellular KSHV genomic DNAs were extracted from harvested cells and quantitated by qPCR as above (B). (*, p

    Article Snippet: For the preparation of DNA from intact virions, 200 μl of virus stocks was pretreated with 2 μl of Turbo DNase I (Ambion) for 1 h at 37°C.

    Techniques: Mutagenesis, BAC Assay, Real-time Polymerase Chain Reaction