tunel assays Search Results


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  • 99
    Thermo Fisher apo brdu tunel assay kit
    Overexpression of murine FAM13A impairs preadipocyte survival. a FAM13A immunofluorescent staining, scale bar, 100 μm. b western blot and c quantification of β-Catenin expression; d qPCR analysis; e MTT viability assay; f Edu incorporation in vector (V) and FAM13A-overexpressing lentivirus transduced 3T3-L1 preadipocytes. g The levels of apoptosis evaluated by FACS analysis following <t>APO-BrdU</t> <t>TUNEL</t> staining combined with LIVE/DEAD cell staining in vector (V) and FAM13A-overexpressing lentivirus transduced 3T3-L1 preadipocytes with or without 24 h serum starvation. The picture shows one of the three experiments. h Numerical results of early and late apoptotic cells from FACS analysis. Values are presented as mean±SEM in 3 separate experiments *: p
    Apo Brdu Tunel Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore tunel
    Assays of EPC function. Cultured <t>EPCs</t> were grown from peripheral blood mononuclear cells as described in the Methods section, and then stained with the isolectin B4 Ulex europaeus agglutinin I to measure their in vitro endothelial differentiation potential. Representative images are shown in ( a ). Original magnification 20X. In ( b ), the in vitro EPC differentiation potential of each individual patient is represented by a dot. In ( c ), 250,000 EPCs were seeded in inserts that were placed in wells of a Boyden companion plate containing VEGF 100 ng/mL in the lower chamber. The number of cells migrating through the 8 μm pore size insert was counted after 4 h. Each dot represents the value for an individual patient. Apoptosis of cultured EPCs was also quantified using <t>TUNEL</t> staining. In ( d ), each dot represents the percentage of apoptotic EPCs for an individual patient. In ( e ), representative TUNEL stained images are shown. Original magnification 20X
    Tunel, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1505 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega deadend fluorometric tunel system
    ZA induces TSC2-null cell apoptosis by GGPP. a MTT assay of TSC2-null cells treated with control, as well as 25, 50, and 100 μM ZA. b Western blot analysis after treatment with ZA (25 and 50 μM, respectively; and 20 nM RAPA for 24 h) in TSC2-null cells. c Immunostaining of Ki67 after treatment with ZA (25 and 50 μM; and 20 nM RAPA for 24 h). Immunofluorescence staining was performed and analyzed in independent 3 experiments. d Apoptosis of TSC2-null cells treated with ZA assayed using the <t>DeadEnd™</t> Fluorometric <t>TUNEL</t> System. The green dots represented apoptotic cells, and DAPI (blue) indicated cell nuclei. Scar bar, 100 and 200 μm, respectively. e Immunoblotting analysis of apoptosis marker (cleaved-caspase3) after treatment with ZA alone, the combination of ZA and FPP, and the combination of ZA and GGPP, respectively, for 24 h. The experiment was performed for three times. All data were presented as mean ± SEM. Compared with the control group, ** P
    Deadend Fluorometric Tunel System, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 6942 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega deadend colorimetric tunel system
    JP-3-110 showed less cytotoxicity to INS-1E cells and human islets than MPA (a) Caspase 3 activities in INS-1E cells after treated with MPA (20µM), MPA+KZ41 (each of 20µM) and JP-3-110 (20µM) for 2 days. Results are presented as the mean ± S.D., n=3. (b) <t>TUNEL</t> assay of INS-1E cells as determined by <t>DeadEnd</t> Colorimetric TUNEL system. Apoptotic cells were stained in dark (arrows). ( c ) JP-3-110 was less toxic to human islets than MPA. Briefly, human islets were cultured with JP-3-110 (20µM) or MPA (20µM) for 5 days. Islets were collected and dispersed with 0.25% Trypsin/EDTA into single cell suspension. Apoptotic cells were stained with FITC labeled annexin V and counted by flow cytometry. P3 indicated the percentage of apoptotic cells. All experiments were performed in triplicates. Results are presented as the mean ± SD. *p
    Deadend Colorimetric Tunel System, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 2164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Beyotime one step tunel apoptosis assay kit
    Activation of MAPKs and PI3K/Akt pathways and increased proliferation, and resistance to <t>apoptosis</t> occurred in BLEL. ( a ) Representative western blot results of MAPKs and PI3K/Akt phosphorylation status are shown. ( b ) Representative western blot results of apoptosis and proliferation markers are shown. ( c ) Immunostaining performed for the proliferation marker Ki-67 are shown (CH: n = 4; BLEL: n = 4). ( d ) Representative images of <t>TUNEL</t> DNA fragmentation assay performed on tissue biopsies of 5 CH and 5 BLEL specimens. Arrowheads in BLEL panel indicated the only few apoptotic cells. ( c and d ) Original magnification: 40×
    One Step Tunel Apoptosis Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 91/100, based on 992 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Promega tunel assay
    Activation of MAPKs and PI3K/Akt pathways and increased proliferation, and resistance to <t>apoptosis</t> occurred in BLEL. ( a ) Representative western blot results of MAPKs and PI3K/Akt phosphorylation status are shown. ( b ) Representative western blot results of apoptosis and proliferation markers are shown. ( c ) Immunostaining performed for the proliferation marker Ki-67 are shown (CH: n = 4; BLEL: n = 4). ( d ) Representative images of <t>TUNEL</t> DNA fragmentation assay performed on tissue biopsies of 5 CH and 5 BLEL specimens. Arrowheads in BLEL panel indicated the only few apoptotic cells. ( c and d ) Original magnification: 40×
    Tunel Assay, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 1643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore tunel assay
    Activation of MAPKs and PI3K/Akt pathways and increased proliferation, and resistance to <t>apoptosis</t> occurred in BLEL. ( a ) Representative western blot results of MAPKs and PI3K/Akt phosphorylation status are shown. ( b ) Representative western blot results of apoptosis and proliferation markers are shown. ( c ) Immunostaining performed for the proliferation marker Ki-67 are shown (CH: n = 4; BLEL: n = 4). ( d ) Representative images of <t>TUNEL</t> DNA fragmentation assay performed on tissue biopsies of 5 CH and 5 BLEL specimens. Arrowheads in BLEL panel indicated the only few apoptotic cells. ( c and d ) Original magnification: 40×
    Tunel Assay, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1509 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher click it plus tunel assay
    <t>TUNEL</t> analysis of DNA-fragmentation in Lop/+ lens. Saggital sections of wild-type (A, B) and Lop/+ (C, D) lenses (P7) showing many TUNEL positive nuclei throughout the central zone of fiber cell degeneration in the Lop/+ lens (C) with only trace levels
    Click It Plus Tunel Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 530 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega deadendtm fluorometric tunel system
    <t>TUNEL</t> analysis of DNA-fragmentation in Lop/+ lens. Saggital sections of wild-type (A, B) and Lop/+ (C, D) lenses (P7) showing many TUNEL positive nuclei throughout the central zone of fiber cell degeneration in the Lop/+ lens (C) with only trace levels
    Deadendtm Fluorometric Tunel System, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 573 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore tunel apoptosis detection kit
    <t>TUNEL</t> and immunohistochemistry analysis of cell <t>apoptosis,</t> and syncytin-1, AIF and calpain1 in tissue sections from preeclamptic ( A ) and normal ( B ) placentas. Brown color indicates the staining signals for target factors. The nuclei were counterstained
    Tunel Apoptosis Detection Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Beyotime tunel assay
    <t>TUNEL</t> and immunohistochemistry analysis of cell <t>apoptosis,</t> and syncytin-1, AIF and calpain1 in tissue sections from preeclamptic ( A ) and normal ( B ) placentas. Brown color indicates the staining signals for target factors. The nuclei were counterstained
    Tunel Assay, supplied by Beyotime, used in various techniques. Bioz Stars score: 94/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Promega tunel staining
    <t>LCAR</t> attenuates the increase in apoptosis induced by oxygen glucose deprivation in rat hippocampal slice cultures. Rat hippocampal slice cultures were exposed to OGD in the presence or absence of LCAR (5 mM, 2 h prior to OGD). After 24 h slices were exposed to the fluorescent caspase activation reagent (10 µM, 20 min) to estimate changes in caspase activity (A). Representative images are shown. Protein extracts (50 µg) were also subjected to Western blot analysis to determine effects on cleaved caspase 9 (CC-9). Each gel was normalized for loading using β-actin. Slices were also analyzed for <t>TUNEL</t> positive nuclei (C). Data are presented as mean ≤ S.E using 8 slices. * = P
    Tunel Staining, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 2889 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega deadend fluorometric tunel system kit
    <t>LCAR</t> attenuates the increase in apoptosis induced by oxygen glucose deprivation in rat hippocampal slice cultures. Rat hippocampal slice cultures were exposed to OGD in the presence or absence of LCAR (5 mM, 2 h prior to OGD). After 24 h slices were exposed to the fluorescent caspase activation reagent (10 µM, 20 min) to estimate changes in caspase activity (A). Representative images are shown. Protein extracts (50 µg) were also subjected to Western blot analysis to determine effects on cleaved caspase 9 (CC-9). Each gel was normalized for loading using β-actin. Slices were also analyzed for <t>TUNEL</t> positive nuclei (C). Data are presented as mean ≤ S.E using 8 slices. * = P
    Deadend Fluorometric Tunel System Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 530 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega tunel assay kit
    Propidium iodide (PI) ( a ), acridine orange (AO) staining ( b ), acridine orange/ethidium bromide (AO/ErBr)-staining ( c ) and terminal <t>deoxynucleotidyl</t> transferase dUTP nick end labeling <t>(TUNEL)</t> assay ( d ) images of vehicle control dimethyl sulfoxide (DMSO), 0.7 µM, 1.4 µM, and 2.8 µM of nymphayol treated MCF-7 cells after 48 h shown at 200x. In PI staining; 1.4 µM and 2.8 µM doses of nymphayol with a horse shoe shaped nucleus and shrunken nucleus confirmed the nuclear pyknosis and apoptosis. In AO/ErBr staining; 2.8 µM dose of nymphayol clearly showed proapoptotic (bright green), early apoptotic (light green) and late apoptotic (orange) cells.
    Tunel Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 404 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Beyotime tunel staining
    RhoA overexpression restored the inhibitory effects of SC on <t>apoptosis</t> in OGD/R-induced PC12 cells. Apoptosis of PC12 cells was evaluated using <t>TUNEL</t> assay (magnification, × 200).
    Tunel Staining, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 471 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Carl Zeiss tunel positive cells
    <t>TUNEL</t> staining of PC3 xenografts Mice bearing bilateral PC3 xenografts were untreated or treated with <t>doxorubicin</t> (i.p.) before receiving intratumoral injections of 500 μg Apo2L/TRAIL (left) or vehicle (right). After 24 hours, tumors were harvested, fixed, processed and analysed for TUNEL staining. Data shown are the mean ± SEM calculated from TUNEL positive cells counted in four fields.
    Tunel Positive Cells, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 792 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Trevigen tunel assay
    <t>TUNEL</t> staining of PC3 xenografts Mice bearing bilateral PC3 xenografts were untreated or treated with <t>doxorubicin</t> (i.p.) before receiving intratumoral injections of 500 μg Apo2L/TRAIL (left) or vehicle (right). After 24 hours, tumors were harvested, fixed, processed and analysed for TUNEL staining. Data shown are the mean ± SEM calculated from TUNEL positive cells counted in four fields.
    Tunel Assay, supplied by Trevigen, used in various techniques. Bioz Stars score: 92/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Trevigen tunel staining
    Protective effect of epicatechin against radiation in rat oral mucosa, as indicated by <t>TUNEL</t> staining for <t>apoptosis.</t> (A) The 4 th day after irradiation, (B) The 9 th day after irradiation, (C) The 23 rd day after irradiation. TUNEL staining of rat mucosa revealed EC treatment significantly decreased radiation-induced TUNEL-positive cells (arrow). Scale bar = 50 µm.
    Tunel Staining, supplied by Trevigen, used in various techniques. Bioz Stars score: 94/100, based on 476 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher click it tunel alexa fluor 488 imaging assay
    Treatment with aspirin‐triggered resolvin D1 ( AT ‐RvD1) and dexamethasone ( DEX ) alone and in combination enhances cell cluster and lumen formation in Par‐C10 cells grown on growth factor‐reduced Matrigel ( GFR ‐ MG ). To establish baseline data, Par‐C10 cells were subjected to single treatments (A–D). Next, cells were preincubated with TNF ‐ α (100 ng/mL) for 1 h (E–I) or 6 h (J–N) and treated for 72 h with AT ‐RvD1(100 ng/mL) and DEX (25–100 ng/mL) alone and in combination. Then, cell clusters were fixed with 4% of paraformaldehyde and stained with rabbit anti‐ ZO ‐1 and <t>Alexa</t> Fluor ® 488 (green), followed by the F‐actin stain Alexa Fluor ® phalloidin 568 (red) and the nuclear stain TO ‐ PRO ® ‐3 iodide (blue). White arrows point to apical localities of ZO ‐1 (2A‐C, E‐N), while the red arrow shows an improper collapsed lumen in cells treated with TNF ‐ α (2D). Images were obtained and analyzed using a Carl Zeiss 710 confocal microscope and ZEN software. All scale bars represent 20 μ m. Lumen sizes were measured following either a 1 h (O) or 6 h (P) preincubation with TNF ‐ α , followed by 72 h treatments with AT ‐RvD1 (100 ng/mL) and DEX (25–100 ng/mL) alone and in combination. Data are expressed as means ± SE of results from 3 or more experiments, where * P
    Click It Tunel Alexa Fluor 488 Imaging Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Keygen Biotech tunel assay
    Treatment with aspirin‐triggered resolvin D1 ( AT ‐RvD1) and dexamethasone ( DEX ) alone and in combination enhances cell cluster and lumen formation in Par‐C10 cells grown on growth factor‐reduced Matrigel ( GFR ‐ MG ). To establish baseline data, Par‐C10 cells were subjected to single treatments (A–D). Next, cells were preincubated with TNF ‐ α (100 ng/mL) for 1 h (E–I) or 6 h (J–N) and treated for 72 h with AT ‐RvD1(100 ng/mL) and DEX (25–100 ng/mL) alone and in combination. Then, cell clusters were fixed with 4% of paraformaldehyde and stained with rabbit anti‐ ZO ‐1 and <t>Alexa</t> Fluor ® 488 (green), followed by the F‐actin stain Alexa Fluor ® phalloidin 568 (red) and the nuclear stain TO ‐ PRO ® ‐3 iodide (blue). White arrows point to apical localities of ZO ‐1 (2A‐C, E‐N), while the red arrow shows an improper collapsed lumen in cells treated with TNF ‐ α (2D). Images were obtained and analyzed using a Carl Zeiss 710 confocal microscope and ZEN software. All scale bars represent 20 μ m. Lumen sizes were measured following either a 1 h (O) or 6 h (P) preincubation with TNF ‐ α , followed by 72 h treatments with AT ‐RvD1 (100 ng/mL) and DEX (25–100 ng/mL) alone and in combination. Data are expressed as means ± SE of results from 3 or more experiments, where * P
    Tunel Assay, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 92/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Overexpression of murine FAM13A impairs preadipocyte survival. a FAM13A immunofluorescent staining, scale bar, 100 μm. b western blot and c quantification of β-Catenin expression; d qPCR analysis; e MTT viability assay; f Edu incorporation in vector (V) and FAM13A-overexpressing lentivirus transduced 3T3-L1 preadipocytes. g The levels of apoptosis evaluated by FACS analysis following APO-BrdU TUNEL staining combined with LIVE/DEAD cell staining in vector (V) and FAM13A-overexpressing lentivirus transduced 3T3-L1 preadipocytes with or without 24 h serum starvation. The picture shows one of the three experiments. h Numerical results of early and late apoptotic cells from FACS analysis. Values are presented as mean±SEM in 3 separate experiments *: p

    Journal: International journal of obesity (2005)

    Article Title: Obesity-associated Family with Sequence Similarity 13, Member A (FAM13A) is Dispensable for Adipose Development and Insulin Sensitivity

    doi: 10.1038/s41366-018-0222-y

    Figure Lengend Snippet: Overexpression of murine FAM13A impairs preadipocyte survival. a FAM13A immunofluorescent staining, scale bar, 100 μm. b western blot and c quantification of β-Catenin expression; d qPCR analysis; e MTT viability assay; f Edu incorporation in vector (V) and FAM13A-overexpressing lentivirus transduced 3T3-L1 preadipocytes. g The levels of apoptosis evaluated by FACS analysis following APO-BrdU TUNEL staining combined with LIVE/DEAD cell staining in vector (V) and FAM13A-overexpressing lentivirus transduced 3T3-L1 preadipocytes with or without 24 h serum starvation. The picture shows one of the three experiments. h Numerical results of early and late apoptotic cells from FACS analysis. Values are presented as mean±SEM in 3 separate experiments *: p

    Article Snippet: Cells were then stained with APO-BrdU TUNEL Assay Kit with Alexa Fluor 488 anti-Brdu (A23210, ThermoFisher Scientific) and LIVE/DEAD Fixable for Far Red Dead Cell Stain Kit, for 633 nm excitation (L34973, ThermoFisher Scientific).

    Techniques: Over Expression, Staining, Western Blot, Expressing, Real-time Polymerase Chain Reaction, MTT Assay, Viability Assay, Plasmid Preparation, FACS, TUNEL Assay

    Assays of EPC function. Cultured EPCs were grown from peripheral blood mononuclear cells as described in the Methods section, and then stained with the isolectin B4 Ulex europaeus agglutinin I to measure their in vitro endothelial differentiation potential. Representative images are shown in ( a ). Original magnification 20X. In ( b ), the in vitro EPC differentiation potential of each individual patient is represented by a dot. In ( c ), 250,000 EPCs were seeded in inserts that were placed in wells of a Boyden companion plate containing VEGF 100 ng/mL in the lower chamber. The number of cells migrating through the 8 μm pore size insert was counted after 4 h. Each dot represents the value for an individual patient. Apoptosis of cultured EPCs was also quantified using TUNEL staining. In ( d ), each dot represents the percentage of apoptotic EPCs for an individual patient. In ( e ), representative TUNEL stained images are shown. Original magnification 20X

    Journal: Canadian Journal of Kidney Health and Disease

    Article Title: Early outgrowth pro-angiogenic cell number and function do not correlate with left ventricular structure and function in conventional hemodialysis patients: a cross-sectional study

    doi: 10.1186/s40697-015-0060-y

    Figure Lengend Snippet: Assays of EPC function. Cultured EPCs were grown from peripheral blood mononuclear cells as described in the Methods section, and then stained with the isolectin B4 Ulex europaeus agglutinin I to measure their in vitro endothelial differentiation potential. Representative images are shown in ( a ). Original magnification 20X. In ( b ), the in vitro EPC differentiation potential of each individual patient is represented by a dot. In ( c ), 250,000 EPCs were seeded in inserts that were placed in wells of a Boyden companion plate containing VEGF 100 ng/mL in the lower chamber. The number of cells migrating through the 8 μm pore size insert was counted after 4 h. Each dot represents the value for an individual patient. Apoptosis of cultured EPCs was also quantified using TUNEL staining. In ( d ), each dot represents the percentage of apoptotic EPCs for an individual patient. In ( e ), representative TUNEL stained images are shown. Original magnification 20X

    Article Snippet: Briefly, 3 × 106 EPCs were seeded on chamber slides and stained with a TUNEL kit (Sigma-Aldrich), followed by nuclear counter-staining with propidium iodide.

    Techniques: Cell Culture, Staining, In Vitro, TUNEL Assay

    ZA induces TSC2-null cell apoptosis by GGPP. a MTT assay of TSC2-null cells treated with control, as well as 25, 50, and 100 μM ZA. b Western blot analysis after treatment with ZA (25 and 50 μM, respectively; and 20 nM RAPA for 24 h) in TSC2-null cells. c Immunostaining of Ki67 after treatment with ZA (25 and 50 μM; and 20 nM RAPA for 24 h). Immunofluorescence staining was performed and analyzed in independent 3 experiments. d Apoptosis of TSC2-null cells treated with ZA assayed using the DeadEnd™ Fluorometric TUNEL System. The green dots represented apoptotic cells, and DAPI (blue) indicated cell nuclei. Scar bar, 100 and 200 μm, respectively. e Immunoblotting analysis of apoptosis marker (cleaved-caspase3) after treatment with ZA alone, the combination of ZA and FPP, and the combination of ZA and GGPP, respectively, for 24 h. The experiment was performed for three times. All data were presented as mean ± SEM. Compared with the control group, ** P

    Journal: Cancer Cell International

    Article Title: Zoledronic acid inhibits TSC2-null cell tumor growth via RhoA/YAP signaling pathway in mouse models of lymphangioleiomyomatosis

    doi: 10.1186/s12935-020-1131-4

    Figure Lengend Snippet: ZA induces TSC2-null cell apoptosis by GGPP. a MTT assay of TSC2-null cells treated with control, as well as 25, 50, and 100 μM ZA. b Western blot analysis after treatment with ZA (25 and 50 μM, respectively; and 20 nM RAPA for 24 h) in TSC2-null cells. c Immunostaining of Ki67 after treatment with ZA (25 and 50 μM; and 20 nM RAPA for 24 h). Immunofluorescence staining was performed and analyzed in independent 3 experiments. d Apoptosis of TSC2-null cells treated with ZA assayed using the DeadEnd™ Fluorometric TUNEL System. The green dots represented apoptotic cells, and DAPI (blue) indicated cell nuclei. Scar bar, 100 and 200 μm, respectively. e Immunoblotting analysis of apoptosis marker (cleaved-caspase3) after treatment with ZA alone, the combination of ZA and FPP, and the combination of ZA and GGPP, respectively, for 24 h. The experiment was performed for three times. All data were presented as mean ± SEM. Compared with the control group, ** P

    Article Snippet: TUNEL assay Apoptotic cells were detected by using DeadEnd™ Fluorometric TUNEL System (Promega, Madison, WI, USA), according to the manufacturer’s instructions.

    Techniques: MTT Assay, Western Blot, Immunostaining, Immunofluorescence, Staining, TUNEL Assay, Marker

    Induction of apoptosis in LS-174T xenografts model A. Treatment Schedule. Mice bearing i.p. LS-174T xenografts were treated with Gem followed by 212 Pb-TCMC-trastuzumab at the indicated time points. Additional groups included untreated, Gem alone, 212 Pb-TCMC-trastuzumab, and 212 Pb-TCMC-HuIgG with/without Gem pre-treatment. B. Apoptosis induced by 212 Pb-TCMC-trastuzumab in response to Gem. The apoptotic nuclei at the indicated time points were counted under light microscopy; 500 nuclei were scored per tumor. Results represent the average of a minimum of three replications (± SD). C. Fluorescence microscopy images of apoptosis at 24 h. Paraffin-embedded sections at 24 h were stained with TUNEL. Top, TUNEL staining; bottom, DAPI counter staining (40x)

    Journal: International journal of radiation oncology, biology, physics

    Article Title: Sensitization of Tumor to 212Pb-radioimmunotherapy by gemcitabine involves initial abrogation of G2 arrest and blocked DNA damage repair by interference with Rad51

    doi: 10.1016/j.ijrobp.2012.09.015

    Figure Lengend Snippet: Induction of apoptosis in LS-174T xenografts model A. Treatment Schedule. Mice bearing i.p. LS-174T xenografts were treated with Gem followed by 212 Pb-TCMC-trastuzumab at the indicated time points. Additional groups included untreated, Gem alone, 212 Pb-TCMC-trastuzumab, and 212 Pb-TCMC-HuIgG with/without Gem pre-treatment. B. Apoptosis induced by 212 Pb-TCMC-trastuzumab in response to Gem. The apoptotic nuclei at the indicated time points were counted under light microscopy; 500 nuclei were scored per tumor. Results represent the average of a minimum of three replications (± SD). C. Fluorescence microscopy images of apoptosis at 24 h. Paraffin-embedded sections at 24 h were stained with TUNEL. Top, TUNEL staining; bottom, DAPI counter staining (40x)

    Article Snippet: The presence of apoptotic bodies on tumor sections was also determined using the Dead End Fluorometric TUNEL System (Promega, Madison, WI).

    Techniques: Mouse Assay, Light Microscopy, Fluorescence, Microscopy, Staining, TUNEL Assay

    Given here are representative histological slides stained with DeadEnd Fluorometric TUNEL. Columns represent the 1-h time point, the final 56-h time point, and the positive control prepared with DNase I. No positive staining is seen at 1 or 56 h, indicating cell death is not via the apoptotic pathway. Scale bar is 20 μ m for all images. To see this figure in color, go online.

    Journal: Biophysical Journal

    Article Title: Monitoring Quantitative Ultrasound Parameter Changes in a Cell Pellet Model of Cell Starvation

    doi: 10.1016/j.bpj.2017.05.017

    Figure Lengend Snippet: Given here are representative histological slides stained with DeadEnd Fluorometric TUNEL. Columns represent the 1-h time point, the final 56-h time point, and the positive control prepared with DNase I. No positive staining is seen at 1 or 56 h, indicating cell death is not via the apoptotic pathway. Scale bar is 20 μ m for all images. To see this figure in color, go online.

    Article Snippet: Slides stained with TUNEL were deparaffinized, as above, and then processed according to the protocol provided with the DeadEnd Fluorometric TUNEL kit (Promega).

    Techniques: Staining, TUNEL Assay, Positive Control

    JP-3-110 showed less cytotoxicity to INS-1E cells and human islets than MPA (a) Caspase 3 activities in INS-1E cells after treated with MPA (20µM), MPA+KZ41 (each of 20µM) and JP-3-110 (20µM) for 2 days. Results are presented as the mean ± S.D., n=3. (b) TUNEL assay of INS-1E cells as determined by DeadEnd Colorimetric TUNEL system. Apoptotic cells were stained in dark (arrows). ( c ) JP-3-110 was less toxic to human islets than MPA. Briefly, human islets were cultured with JP-3-110 (20µM) or MPA (20µM) for 5 days. Islets were collected and dispersed with 0.25% Trypsin/EDTA into single cell suspension. Apoptotic cells were stained with FITC labeled annexin V and counted by flow cytometry. P3 indicated the percentage of apoptotic cells. All experiments were performed in triplicates. Results are presented as the mean ± SD. *p

    Journal: Bioconjugate chemistry

    Article Title: Synthesis and Characterization of an Antiapoptotic Immunosuppressive Compound for improving the Outcome of Islet Transplantation

    doi: 10.1021/bc400369t

    Figure Lengend Snippet: JP-3-110 showed less cytotoxicity to INS-1E cells and human islets than MPA (a) Caspase 3 activities in INS-1E cells after treated with MPA (20µM), MPA+KZ41 (each of 20µM) and JP-3-110 (20µM) for 2 days. Results are presented as the mean ± S.D., n=3. (b) TUNEL assay of INS-1E cells as determined by DeadEnd Colorimetric TUNEL system. Apoptotic cells were stained in dark (arrows). ( c ) JP-3-110 was less toxic to human islets than MPA. Briefly, human islets were cultured with JP-3-110 (20µM) or MPA (20µM) for 5 days. Islets were collected and dispersed with 0.25% Trypsin/EDTA into single cell suspension. Apoptotic cells were stained with FITC labeled annexin V and counted by flow cytometry. P3 indicated the percentage of apoptotic cells. All experiments were performed in triplicates. Results are presented as the mean ± SD. *p

    Article Snippet: After the same treatment mentioned before, INS-1E cells were characterized by DeadEnd™ Colorimetric TUNEL system (Promega, Madison, WI), in which fragmented DNA from apoptotic cells was labeled with biotinylated nucleotide and detected using hydrogen peroxide and diaminobenzidine.

    Techniques: TUNEL Assay, Staining, Cell Culture, Labeling, Flow Cytometry, Cytometry

    Activation of MAPKs and PI3K/Akt pathways and increased proliferation, and resistance to apoptosis occurred in BLEL. ( a ) Representative western blot results of MAPKs and PI3K/Akt phosphorylation status are shown. ( b ) Representative western blot results of apoptosis and proliferation markers are shown. ( c ) Immunostaining performed for the proliferation marker Ki-67 are shown (CH: n = 4; BLEL: n = 4). ( d ) Representative images of TUNEL DNA fragmentation assay performed on tissue biopsies of 5 CH and 5 BLEL specimens. Arrowheads in BLEL panel indicated the only few apoptotic cells. ( c and d ) Original magnification: 40×

    Journal: Cell Communication and Signaling : CCS

    Article Title: Macrophage migration inhibitory factor contributes to the pathogenesis of benign lymphoepithelial lesion of the lacrimal gland

    doi: 10.1186/s12964-018-0284-4

    Figure Lengend Snippet: Activation of MAPKs and PI3K/Akt pathways and increased proliferation, and resistance to apoptosis occurred in BLEL. ( a ) Representative western blot results of MAPKs and PI3K/Akt phosphorylation status are shown. ( b ) Representative western blot results of apoptosis and proliferation markers are shown. ( c ) Immunostaining performed for the proliferation marker Ki-67 are shown (CH: n = 4; BLEL: n = 4). ( d ) Representative images of TUNEL DNA fragmentation assay performed on tissue biopsies of 5 CH and 5 BLEL specimens. Arrowheads in BLEL panel indicated the only few apoptotic cells. ( c and d ) Original magnification: 40×

    Article Snippet: TUNEL assay Apoptosis analysis in cell cultures and paraffin-embedded tissue sections were performed using the one-step TUNEL apoptosis assay kit in accordance with the manufacturer’s protocol (Beyotime Biotechnology, Shanghai, China).

    Techniques: Activation Assay, Western Blot, Immunostaining, Marker, TUNEL Assay, DNA Fragmentation Assay

    TUNEL analysis of DNA-fragmentation in Lop/+ lens. Saggital sections of wild-type (A, B) and Lop/+ (C, D) lenses (P7) showing many TUNEL positive nuclei throughout the central zone of fiber cell degeneration in the Lop/+ lens (C) with only trace levels

    Journal: Biochimica et biophysica acta

    Article Title: Lens ER-stress response during cataract development in Mip-mutant mice

    doi: 10.1016/j.bbadis.2016.05.003

    Figure Lengend Snippet: TUNEL analysis of DNA-fragmentation in Lop/+ lens. Saggital sections of wild-type (A, B) and Lop/+ (C, D) lenses (P7) showing many TUNEL positive nuclei throughout the central zone of fiber cell degeneration in the Lop/+ lens (C) with only trace levels

    Article Snippet: DNA double-strand breaks (3′-OH ends) were detected in mouse lenses (P7) using the Click-iT Plus TUNEL assay according to the manufacturer's protocol (ThermoFisher).

    Techniques: TUNEL Assay

    TUNEL and immunohistochemistry analysis of cell apoptosis, and syncytin-1, AIF and calpain1 in tissue sections from preeclamptic ( A ) and normal ( B ) placentas. Brown color indicates the staining signals for target factors. The nuclei were counterstained

    Journal: Cellular and molecular life sciences : CMLS

    Article Title: Reduced syncytin-1 expression in chriocarcinoma BeWo cells activates the calpain1-AIF-mediated apoptosis, implication for preeclampsia

    doi: 10.1007/s00018-013-1533-8

    Figure Lengend Snippet: TUNEL and immunohistochemistry analysis of cell apoptosis, and syncytin-1, AIF and calpain1 in tissue sections from preeclamptic ( A ) and normal ( B ) placentas. Brown color indicates the staining signals for target factors. The nuclei were counterstained

    Article Snippet: TUNEL Apoptosis Detection Kit (Millipore, Billerica, MA, USA) was used to detect apoptotic cells in placental tissue sections.

    Techniques: TUNEL Assay, Immunohistochemistry, Staining

    Syncytin-1 knockdown-induced BeWo cell apoptosis. ( A ) Representative results of TUNEL assay (40X). BeWo cells transfected with control (Panel 1) or syncytin-1 specific siRNA (panel 2) were examined with TUNEL for apoptosis at 48 hours post-transfection.

    Journal: Cellular and molecular life sciences : CMLS

    Article Title: Reduced syncytin-1 expression in chriocarcinoma BeWo cells activates the calpain1-AIF-mediated apoptosis, implication for preeclampsia

    doi: 10.1007/s00018-013-1533-8

    Figure Lengend Snippet: Syncytin-1 knockdown-induced BeWo cell apoptosis. ( A ) Representative results of TUNEL assay (40X). BeWo cells transfected with control (Panel 1) or syncytin-1 specific siRNA (panel 2) were examined with TUNEL for apoptosis at 48 hours post-transfection.

    Article Snippet: TUNEL Apoptosis Detection Kit (Millipore, Billerica, MA, USA) was used to detect apoptotic cells in placental tissue sections.

    Techniques: TUNEL Assay, Transfection

    LCAR attenuates the increase in apoptosis induced by oxygen glucose deprivation in rat hippocampal slice cultures. Rat hippocampal slice cultures were exposed to OGD in the presence or absence of LCAR (5 mM, 2 h prior to OGD). After 24 h slices were exposed to the fluorescent caspase activation reagent (10 µM, 20 min) to estimate changes in caspase activity (A). Representative images are shown. Protein extracts (50 µg) were also subjected to Western blot analysis to determine effects on cleaved caspase 9 (CC-9). Each gel was normalized for loading using β-actin. Slices were also analyzed for TUNEL positive nuclei (C). Data are presented as mean ≤ S.E using 8 slices. * = P

    Journal: PLoS ONE

    Article Title: Oxygen Glucose Deprivation in Rat Hippocampal Slice Cultures Results in Alterations in Carnitine Homeostasis and Mitochondrial Dysfunction

    doi: 10.1371/journal.pone.0040881

    Figure Lengend Snippet: LCAR attenuates the increase in apoptosis induced by oxygen glucose deprivation in rat hippocampal slice cultures. Rat hippocampal slice cultures were exposed to OGD in the presence or absence of LCAR (5 mM, 2 h prior to OGD). After 24 h slices were exposed to the fluorescent caspase activation reagent (10 µM, 20 min) to estimate changes in caspase activity (A). Representative images are shown. Protein extracts (50 µg) were also subjected to Western blot analysis to determine effects on cleaved caspase 9 (CC-9). Each gel was normalized for loading using β-actin. Slices were also analyzed for TUNEL positive nuclei (C). Data are presented as mean ≤ S.E using 8 slices. * = P

    Article Snippet: Our data indicate that LCAR supplementation significantly decreased TUNEL staining of neurons 24 h post reperfusion ( C).

    Techniques: Activation Assay, Activity Assay, Western Blot, TUNEL Assay

    Propidium iodide (PI) ( a ), acridine orange (AO) staining ( b ), acridine orange/ethidium bromide (AO/ErBr)-staining ( c ) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay ( d ) images of vehicle control dimethyl sulfoxide (DMSO), 0.7 µM, 1.4 µM, and 2.8 µM of nymphayol treated MCF-7 cells after 48 h shown at 200x. In PI staining; 1.4 µM and 2.8 µM doses of nymphayol with a horse shoe shaped nucleus and shrunken nucleus confirmed the nuclear pyknosis and apoptosis. In AO/ErBr staining; 2.8 µM dose of nymphayol clearly showed proapoptotic (bright green), early apoptotic (light green) and late apoptotic (orange) cells.

    Journal: Metabolites

    Article Title: Potential Metabolite Nymphayol Isolated from Water Lily (Nymphaea stellata) Flower Inhibits MCF-7 Human Breast Cancer Cell Growth via Upregulation of Cdkn2a, pRb2, p53 and Downregulation of PCNA mRNA Expressions

    doi: 10.3390/metabo10070280

    Figure Lengend Snippet: Propidium iodide (PI) ( a ), acridine orange (AO) staining ( b ), acridine orange/ethidium bromide (AO/ErBr)-staining ( c ) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay ( d ) images of vehicle control dimethyl sulfoxide (DMSO), 0.7 µM, 1.4 µM, and 2.8 µM of nymphayol treated MCF-7 cells after 48 h shown at 200x. In PI staining; 1.4 µM and 2.8 µM doses of nymphayol with a horse shoe shaped nucleus and shrunken nucleus confirmed the nuclear pyknosis and apoptosis. In AO/ErBr staining; 2.8 µM dose of nymphayol clearly showed proapoptotic (bright green), early apoptotic (light green) and late apoptotic (orange) cells.

    Article Snippet: The DeadEnd Terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) Assay Kit was procured from Promega (Madison, WI, USA).

    Techniques: Staining, TUNEL Assay

    Silibinin targets proliferation, apoptosis, hypoxia, lipogenesis, and PSA level in 22Rv1 xenograft tissues. 22Rv1 xenografts were analyzed by IHC for Ki-67, cyclin D1, TUNEL, CD31, HIF-1α, FASN, ACC and PSA. Percentage of Ki-67, cyclin D1, TUNEL, HIF-1α and microvessel density (CD31 positive microvessel) were measured at five arbitrarily selected fields from each tumor at 400× magnification. Immunoreactivity for FASN, ACC and PSA was analyzed in 5 random areas for each tumor tissue and was scored as 0+ (no staining), 1+ (weak staining), 2+ (moderate staining), 3+ (strong staining), 4+ (very strong staining). Each value in the bar diagram is mean ± SEM of 10–12 xenografts. *, p ≤ 0.001

    Journal: Molecular carcinogenesis

    Article Title: Silibinin inhibits hypoxia-induced HIF-1α-mediated signaling, angiogenesis and lipogenesis in prostate cancer cells: In vitro evidence and in vivo functional imaging and metabolomics

    doi: 10.1002/mc.22537

    Figure Lengend Snippet: Silibinin targets proliferation, apoptosis, hypoxia, lipogenesis, and PSA level in 22Rv1 xenograft tissues. 22Rv1 xenografts were analyzed by IHC for Ki-67, cyclin D1, TUNEL, CD31, HIF-1α, FASN, ACC and PSA. Percentage of Ki-67, cyclin D1, TUNEL, HIF-1α and microvessel density (CD31 positive microvessel) were measured at five arbitrarily selected fields from each tumor at 400× magnification. Immunoreactivity for FASN, ACC and PSA was analyzed in 5 random areas for each tumor tissue and was scored as 0+ (no staining), 1+ (weak staining), 2+ (moderate staining), 3+ (strong staining), 4+ (very strong staining). Each value in the bar diagram is mean ± SEM of 10–12 xenografts. *, p ≤ 0.001

    Article Snippet: Ki-67 and CD31 antibodies were from Abcam (Cambridge, MA), cyclin D1 antibody was from Thermo Scientific (Fremont, CA), TUNEL kit was from Promega (Madison, WI), PSA (prostate specific antigen) antibody was from DAKO (Carpinteria, CA), and HIF-1α antibody used for immunohistochemistry (IHC) was from Santa Cruz Biotechnology (Dallas, TX).

    Techniques: Immunohistochemistry, TUNEL Assay, Staining

    RhoA overexpression restored the inhibitory effects of SC on apoptosis in OGD/R-induced PC12 cells. Apoptosis of PC12 cells was evaluated using TUNEL assay (magnification, × 200).

    Journal: Inflammation

    Article Title: Sanggenon C Ameliorates Cerebral Ischemia-Reperfusion Injury by Inhibiting Inflammation and Oxidative Stress through Regulating RhoA-ROCK Signaling

    doi: 10.1007/s10753-020-01225-w

    Figure Lengend Snippet: RhoA overexpression restored the inhibitory effects of SC on apoptosis in OGD/R-induced PC12 cells. Apoptosis of PC12 cells was evaluated using TUNEL assay (magnification, × 200).

    Article Snippet: SC Decreased Cell Apoptosis Induced by MCAO-Reperfusion To explore whether SC could protect brain cells from apoptosis after MCAO-reperfusion, we performed TUNEL staining in rat brain slices that were obtained 24 h after MCAO-reperfusion.

    Techniques: Over Expression, TUNEL Assay

    SC decreased cell apoptosis induced by MCAO-reperfusion. a Apoptosis of cells was examined using TUNEL assay. (magnification, × 200). b The expression of apoptosis-related proteins was determined using western blot analysis. ** P

    Journal: Inflammation

    Article Title: Sanggenon C Ameliorates Cerebral Ischemia-Reperfusion Injury by Inhibiting Inflammation and Oxidative Stress through Regulating RhoA-ROCK Signaling

    doi: 10.1007/s10753-020-01225-w

    Figure Lengend Snippet: SC decreased cell apoptosis induced by MCAO-reperfusion. a Apoptosis of cells was examined using TUNEL assay. (magnification, × 200). b The expression of apoptosis-related proteins was determined using western blot analysis. ** P

    Article Snippet: SC Decreased Cell Apoptosis Induced by MCAO-Reperfusion To explore whether SC could protect brain cells from apoptosis after MCAO-reperfusion, we performed TUNEL staining in rat brain slices that were obtained 24 h after MCAO-reperfusion.

    Techniques: TUNEL Assay, Expressing, Western Blot

    TUNEL staining of PC3 xenografts Mice bearing bilateral PC3 xenografts were untreated or treated with doxorubicin (i.p.) before receiving intratumoral injections of 500 μg Apo2L/TRAIL (left) or vehicle (right). After 24 hours, tumors were harvested, fixed, processed and analysed for TUNEL staining. Data shown are the mean ± SEM calculated from TUNEL positive cells counted in four fields.

    Journal: BMC Cancer

    Article Title: Doxorubicin increases the effectiveness of Apo2L/TRAIL for tumor growth inhibition of prostate cancer xenografts

    doi: 10.1186/1471-2407-5-2

    Figure Lengend Snippet: TUNEL staining of PC3 xenografts Mice bearing bilateral PC3 xenografts were untreated or treated with doxorubicin (i.p.) before receiving intratumoral injections of 500 μg Apo2L/TRAIL (left) or vehicle (right). After 24 hours, tumors were harvested, fixed, processed and analysed for TUNEL staining. Data shown are the mean ± SEM calculated from TUNEL positive cells counted in four fields.

    Article Snippet: However, combination treatment of doxorubicin and Apo2L/TRAIL yielded the most TUNEL positive cells (278% compared to the control) and was statistically different from all other treatment groups.

    Techniques: TUNEL Assay, Staining, Mouse Assay

    Protective effect of epicatechin against radiation in rat oral mucosa, as indicated by TUNEL staining for apoptosis. (A) The 4 th day after irradiation, (B) The 9 th day after irradiation, (C) The 23 rd day after irradiation. TUNEL staining of rat mucosa revealed EC treatment significantly decreased radiation-induced TUNEL-positive cells (arrow). Scale bar = 50 µm.

    Journal: PLoS ONE

    Article Title: Effect of Epicatechin against Radiation-Induced Oral Mucositis: In Vitro and In Vivo Study

    doi: 10.1371/journal.pone.0069151

    Figure Lengend Snippet: Protective effect of epicatechin against radiation in rat oral mucosa, as indicated by TUNEL staining for apoptosis. (A) The 4 th day after irradiation, (B) The 9 th day after irradiation, (C) The 23 rd day after irradiation. TUNEL staining of rat mucosa revealed EC treatment significantly decreased radiation-induced TUNEL-positive cells (arrow). Scale bar = 50 µm.

    Article Snippet: TUNEL Staining in Rats Apoptosis of oral mucosa was determined by the TUNEL method using an in situ detection kit (TACS 2 TdT DAB kit; Trevigen, Gaithersburg, MD, USA) according to the manufacturer’s instructions.

    Techniques: TUNEL Assay, Staining, Irradiation

    Protective effect of epicatechin against radiation toxicity in HaCaT cells. (A) TUNEL assay of treated HaCaT cells. EC reduced radiation-induced apoptosis of HaCaT cells. Scale bar = 50 µm. (B) Quantification of percentage of apoptosis seen in flow cytometry of Annexin V-FITC and PI double stained HaCaT cells. The data represent the mean ± SD of three independent experiments. ** p

    Journal: PLoS ONE

    Article Title: Effect of Epicatechin against Radiation-Induced Oral Mucositis: In Vitro and In Vivo Study

    doi: 10.1371/journal.pone.0069151

    Figure Lengend Snippet: Protective effect of epicatechin against radiation toxicity in HaCaT cells. (A) TUNEL assay of treated HaCaT cells. EC reduced radiation-induced apoptosis of HaCaT cells. Scale bar = 50 µm. (B) Quantification of percentage of apoptosis seen in flow cytometry of Annexin V-FITC and PI double stained HaCaT cells. The data represent the mean ± SD of three independent experiments. ** p

    Article Snippet: TUNEL Staining in Rats Apoptosis of oral mucosa was determined by the TUNEL method using an in situ detection kit (TACS 2 TdT DAB kit; Trevigen, Gaithersburg, MD, USA) according to the manufacturer’s instructions.

    Techniques: TUNEL Assay, Flow Cytometry, Cytometry, Staining

    Treatment with aspirin‐triggered resolvin D1 ( AT ‐RvD1) and dexamethasone ( DEX ) alone and in combination enhances cell cluster and lumen formation in Par‐C10 cells grown on growth factor‐reduced Matrigel ( GFR ‐ MG ). To establish baseline data, Par‐C10 cells were subjected to single treatments (A–D). Next, cells were preincubated with TNF ‐ α (100 ng/mL) for 1 h (E–I) or 6 h (J–N) and treated for 72 h with AT ‐RvD1(100 ng/mL) and DEX (25–100 ng/mL) alone and in combination. Then, cell clusters were fixed with 4% of paraformaldehyde and stained with rabbit anti‐ ZO ‐1 and Alexa Fluor ® 488 (green), followed by the F‐actin stain Alexa Fluor ® phalloidin 568 (red) and the nuclear stain TO ‐ PRO ® ‐3 iodide (blue). White arrows point to apical localities of ZO ‐1 (2A‐C, E‐N), while the red arrow shows an improper collapsed lumen in cells treated with TNF ‐ α (2D). Images were obtained and analyzed using a Carl Zeiss 710 confocal microscope and ZEN software. All scale bars represent 20 μ m. Lumen sizes were measured following either a 1 h (O) or 6 h (P) preincubation with TNF ‐ α , followed by 72 h treatments with AT ‐RvD1 (100 ng/mL) and DEX (25–100 ng/mL) alone and in combination. Data are expressed as means ± SE of results from 3 or more experiments, where * P

    Journal: Physiological Reports

    Article Title: AT‐RvD1 combined with DEX is highly effective in treating TNF‐α‐mediated disruption of the salivary gland epithelium. AT‐RvD1 combined with DEX is highly effective in treating TNF‐α‐mediated disruption of salivary gland epithelium

    doi: 10.14814/phy2.12990

    Figure Lengend Snippet: Treatment with aspirin‐triggered resolvin D1 ( AT ‐RvD1) and dexamethasone ( DEX ) alone and in combination enhances cell cluster and lumen formation in Par‐C10 cells grown on growth factor‐reduced Matrigel ( GFR ‐ MG ). To establish baseline data, Par‐C10 cells were subjected to single treatments (A–D). Next, cells were preincubated with TNF ‐ α (100 ng/mL) for 1 h (E–I) or 6 h (J–N) and treated for 72 h with AT ‐RvD1(100 ng/mL) and DEX (25–100 ng/mL) alone and in combination. Then, cell clusters were fixed with 4% of paraformaldehyde and stained with rabbit anti‐ ZO ‐1 and Alexa Fluor ® 488 (green), followed by the F‐actin stain Alexa Fluor ® phalloidin 568 (red) and the nuclear stain TO ‐ PRO ® ‐3 iodide (blue). White arrows point to apical localities of ZO ‐1 (2A‐C, E‐N), while the red arrow shows an improper collapsed lumen in cells treated with TNF ‐ α (2D). Images were obtained and analyzed using a Carl Zeiss 710 confocal microscope and ZEN software. All scale bars represent 20 μ m. Lumen sizes were measured following either a 1 h (O) or 6 h (P) preincubation with TNF ‐ α , followed by 72 h treatments with AT ‐RvD1 (100 ng/mL) and DEX (25–100 ng/mL) alone and in combination. Data are expressed as means ± SE of results from 3 or more experiments, where * P

    Article Snippet: TUNEL analysis Par‐C10 cells (passages 52–57) were plated on GFR‐MG as described above and preincubated with TNF‐α for 1 h. Cells were then treated with AT‐RvD1 (100 ng/mL) and varying doses of DEX (25–100 ng/mL) both alone and in combination for 72 h. A terminal deoxynucelotidyl transferase‐mediated dUTP nick end‐labeling (TUNEL) assay was performed with a Click‐It® TUNEL Alexa Fluor® 488 imaging assay (Life Technologies, Grand Island, NY) as described previously (Easley et al. ).

    Techniques: Staining, Microscopy, Software