ts k1 dna polymerase Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    New England Biolabs ts k1 dna polymerase
    Phylogenetic tree showing the evolutionary relationship between Ts <t>K1</t> DNA polymerase and other Thermus DNA polymerases based on maximum likelihood analysis. The tree with the highest likelihood (7699.30) is shown, and the bootstrap value (1000 replicates) for each clade is shown next to each branch. The final dataset contained 824 positions. All positions containing gaps or missing data were eliminated (complete deletion option). Bar indicates 0.05 substitutions per amino acid position. Evolutionary analyses were conducted using MEGA X
    Ts K1 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ts k1 dna polymerase/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ts k1 dna polymerase - by Bioz Stars, 2022-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    Phylogenetic tree showing the evolutionary relationship between Ts K1 DNA polymerase and other Thermus DNA polymerases based on maximum likelihood analysis. The tree with the highest likelihood (7699.30) is shown, and the bootstrap value (1000 replicates) for each clade is shown next to each branch. The final dataset contained 824 positions. All positions containing gaps or missing data were eliminated (complete deletion option). Bar indicates 0.05 substitutions per amino acid position. Evolutionary analyses were conducted using MEGA X

    Journal: MicrobiologyOpen

    Article Title: Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1

    doi: 10.1002/mbo3.1149

    Figure Lengend Snippet: Phylogenetic tree showing the evolutionary relationship between Ts K1 DNA polymerase and other Thermus DNA polymerases based on maximum likelihood analysis. The tree with the highest likelihood (7699.30) is shown, and the bootstrap value (1000 replicates) for each clade is shown next to each branch. The final dataset contained 824 positions. All positions containing gaps or missing data were eliminated (complete deletion option). Bar indicates 0.05 substitutions per amino acid position. Evolutionary analyses were conducted using MEGA X

    Article Snippet: Although the half‐life of Ts K1 DNA polymerase (without the use of additional stabilizers) at 95°C (15 min) is lower than that of Taq (Lawyer et al., ), it is close to another commercially available rTaq (20 min) polymerase (Gibbs et al., ) suggesting that it could still be used in PCR based applications.

    Techniques:

    Properties of Ts K1 DNA polymerase. (a) Effect of temperature on Ts K1 DNA polymerase activity; (b) effect of pH on Ts K1 DNA polymerase activity in MOPS‐NaOH (■), Tris‐HCl (▲), and glycine‐NaOH (●) buffers; (c) effect of KCl concentration on Ts K1 DNA polymerase activity; and (d) effect of the divalent cations Mg 2+ (■) and Mn 2+ (●) on Ts K1 DNA polymerase activity. Each point represents the average of 3 measured values, and error bars represent the standard deviation between these 3 values

    Journal: MicrobiologyOpen

    Article Title: Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1

    doi: 10.1002/mbo3.1149

    Figure Lengend Snippet: Properties of Ts K1 DNA polymerase. (a) Effect of temperature on Ts K1 DNA polymerase activity; (b) effect of pH on Ts K1 DNA polymerase activity in MOPS‐NaOH (■), Tris‐HCl (▲), and glycine‐NaOH (●) buffers; (c) effect of KCl concentration on Ts K1 DNA polymerase activity; and (d) effect of the divalent cations Mg 2+ (■) and Mn 2+ (●) on Ts K1 DNA polymerase activity. Each point represents the average of 3 measured values, and error bars represent the standard deviation between these 3 values

    Article Snippet: Although the half‐life of Ts K1 DNA polymerase (without the use of additional stabilizers) at 95°C (15 min) is lower than that of Taq (Lawyer et al., ), it is close to another commercially available rTaq (20 min) polymerase (Gibbs et al., ) suggesting that it could still be used in PCR based applications.

    Techniques: Activity Assay, Concentration Assay, Standard Deviation

    Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of Ts K1 DNA polymerase purification: (1) noninduced culture, (2) induced culture, (3) sonicated extract from host cells, (4) supernatant after heat treatment, 5) purified protein, M1—Full Range Rainbow molecular‐mass marker (Amersham)

    Journal: MicrobiologyOpen

    Article Title: Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1

    doi: 10.1002/mbo3.1149

    Figure Lengend Snippet: Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of Ts K1 DNA polymerase purification: (1) noninduced culture, (2) induced culture, (3) sonicated extract from host cells, (4) supernatant after heat treatment, 5) purified protein, M1—Full Range Rainbow molecular‐mass marker (Amersham)

    Article Snippet: Although the half‐life of Ts K1 DNA polymerase (without the use of additional stabilizers) at 95°C (15 min) is lower than that of Taq (Lawyer et al., ), it is close to another commercially available rTaq (20 min) polymerase (Gibbs et al., ) suggesting that it could still be used in PCR based applications.

    Techniques: Polyacrylamide Gel Electrophoresis, Purification, Sonication, Marker

    Polymerase chain reaction products amplified using Ts K1 DNA polymerase. Lane 1, 265 bp; lane 2, 500 bp; lane 3, 1500 bp; lane 4, 1920 bp; lane 5, 2.5 kb; M, GeneRuler 1 kb Plus DNA ladder (New England BioLabs)

    Journal: MicrobiologyOpen

    Article Title: Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1

    doi: 10.1002/mbo3.1149

    Figure Lengend Snippet: Polymerase chain reaction products amplified using Ts K1 DNA polymerase. Lane 1, 265 bp; lane 2, 500 bp; lane 3, 1500 bp; lane 4, 1920 bp; lane 5, 2.5 kb; M, GeneRuler 1 kb Plus DNA ladder (New England BioLabs)

    Article Snippet: Although the half‐life of Ts K1 DNA polymerase (without the use of additional stabilizers) at 95°C (15 min) is lower than that of Taq (Lawyer et al., ), it is close to another commercially available rTaq (20 min) polymerase (Gibbs et al., ) suggesting that it could still be used in PCR based applications.

    Techniques: Polymerase Chain Reaction, Amplification

    Ts K1 DNA polymerase thermostability. ×Represents 75°C, ▲ represents 80°C, ● represents 88°C, ♦ represents 95°C. Data are represented on a logarithmic scale. Each point represents the average of 3 measured values, and error bars represent the standard deviation between these 3 values

    Journal: MicrobiologyOpen

    Article Title: Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1

    doi: 10.1002/mbo3.1149

    Figure Lengend Snippet: Ts K1 DNA polymerase thermostability. ×Represents 75°C, ▲ represents 80°C, ● represents 88°C, ♦ represents 95°C. Data are represented on a logarithmic scale. Each point represents the average of 3 measured values, and error bars represent the standard deviation between these 3 values

    Article Snippet: Although the half‐life of Ts K1 DNA polymerase (without the use of additional stabilizers) at 95°C (15 min) is lower than that of Taq (Lawyer et al., ), it is close to another commercially available rTaq (20 min) polymerase (Gibbs et al., ) suggesting that it could still be used in PCR based applications.

    Techniques: Standard Deviation