tryptose phosphate broth Search Results


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  • 99
    Thermo Fisher tryptose phosphate broth
    Tryptose Phosphate Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 558 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore tryptose phosphate broth
    Quantification of knockdown of chANP32A in chicken cells <t>DF-1</t> cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .
    Tryptose Phosphate Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 975 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco tryptose phosphate broth
    Quantification of knockdown of chANP32A in chicken cells <t>DF-1</t> cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .
    Tryptose Phosphate Broth, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Millipore tryptose phosphate broth solution
    Quantification of knockdown of chANP32A in chicken cells <t>DF-1</t> cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .
    Tryptose Phosphate Broth Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson tryptose phosphate broth
    Quantification of knockdown of chANP32A in chicken cells <t>DF-1</t> cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .
    Tryptose Phosphate Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 146 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Difco bacto tryptone phosphate broth
    Quantification of knockdown of chANP32A in chicken cells <t>DF-1</t> cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .
    Bacto Tryptone Phosphate Broth, supplied by Difco, used in various techniques. Bioz Stars score: 91/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco bacto tryptose phosphate broth
    Quantification of knockdown of chANP32A in chicken cells <t>DF-1</t> cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .
    Bacto Tryptose Phosphate Broth, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Valiant tryptose phosphate broth
    Quantification of knockdown of chANP32A in chicken cells <t>DF-1</t> cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .
    Tryptose Phosphate Broth, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eurobio tryptose phosphate broth
    Quantification of knockdown of chANP32A in chicken cells <t>DF-1</t> cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .
    Tryptose Phosphate Broth, supplied by Eurobio, used in various techniques. Bioz Stars score: 92/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore tryptone phosphate broth
    Quantification of knockdown of chANP32A in chicken cells <t>DF-1</t> cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .
    Tryptone Phosphate Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HiMedia Laboratories tryptose phosphate broth
    Quantification of knockdown of chANP32A in chicken cells <t>DF-1</t> cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .
    Tryptose Phosphate Broth, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Becton Dickinson bacto tryptose phosphate broth
    Quantification of knockdown of chANP32A in chicken cells <t>DF-1</t> cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .
    Bacto Tryptose Phosphate Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson bactotm tryptose phosphate broth
    Quantification of knockdown of chANP32A in chicken cells <t>DF-1</t> cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .
    Bactotm Tryptose Phosphate Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson tryptone phosphate broth
    Quantification of knockdown of chANP32A in chicken cells <t>DF-1</t> cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .
    Tryptone Phosphate Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Quantification of knockdown of chANP32A in chicken cells DF-1 cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .

    Journal: Nature

    Article Title: Species difference in ANP32A underlies influenza A virus polymerase host restriction

    doi: 10.1038/nature16474

    Figure Lengend Snippet: Quantification of knockdown of chANP32A in chicken cells DF-1 cells were transduced with VSV-G lentiviral vectors that delivered a transgene expressing shRNA directed against chANP32A or a negative sequence and the puromycin gene. Puromycin selected cells were transfected with siRNA (100nM) (underlined). RNA was extracted from untreated shRNA cells and siRNA-treated shRNA cells. Knockdown of chANP32A was quantified by qRT-PCR of the extracted RNA, calculated using a standard curve generated with chANP32A cDNA, using primers specific for chANP32A. Fold decrease of RNA copies is displayed as compared to Negative shRNA DF-1 or ALLstars treated chANP32A shRNA DF-1 cells. (Error displayed as SEM; n=3 biological replicates). This analysis accompanies experiments in Figure 3a-c .

    Article Snippet: Chicken fibroblast (DF-1) (ATCC) cells were maintained in DF-1 cell culture media (DMEM supplemented with 10% FCS, 5% tryptose phosphate broth (Sigma-Aldrich) and 0.1% Penicillin-streptomycin (Invitrogen)).

    Techniques: Transduction, Expressing, shRNA, Sequencing, Transfection, Quantitative RT-PCR, Generated

    Knockdown of ANP32 reveals avian influenza polymerase dependence on chANP32A and dependence on huANP32A and B in human cells by human adapted influenza polymerase a, DF-1 cells transduced with VSV-G lentiviral vectors delivering transgenes expressing puromycin and shRNA targeting chANP32A or Negative. Puromycin selected cells transfected with pCOM1-firefly minigenome reporter, avian 50-92 polymerase (627E), and Renilla expression control . b, siRNA (100nM) applied to DF-1 chANP32A shRNA cells. After 48hrs, cells transfected with avian 50-92 polymerase (627E), minigenome reporter and Renilla expression control. Luciferase activity measured 20hrs later. Knockdown in chicken DF-1 cells verified by immunoblotting using antibody against vinculin and chANP32A. c, DF-1 cells depleted of chANP32A by siRNA infected with avian-like influenza virus (PR8 virus bearing H5N1 Ty05 polymerase genes with PB2 627E, MOI 0.01). 24hrs later cell supernatants titrated for infectious virus by plaque assay on MDCK cells. d , 293T cells transduced with lentiviral vectors delivering transgenes expressing puromycin and shRNA targeting huANP32A, huANP32B, both huANP32A and B, or Negative. Puromycin selected cells transfected with, pHOM1-firefly minigenome reporter, human-adapted avian 50-92 polymerase (627K), and Renilla expression control.Luciferase activity measured after 20hrs. Knockdown in 293T cells verified immunoblotting using antibody against vinculin, huANP32A and huANP32B. (a,b d, data are firefly activity normalised to Renilla, plotted as % of Negative or Allstars; error as SEM; One-way ANOVA comparisons to ALLStars or Negative, ns= not significant, **p

    Journal: Nature

    Article Title: Species difference in ANP32A underlies influenza A virus polymerase host restriction

    doi: 10.1038/nature16474

    Figure Lengend Snippet: Knockdown of ANP32 reveals avian influenza polymerase dependence on chANP32A and dependence on huANP32A and B in human cells by human adapted influenza polymerase a, DF-1 cells transduced with VSV-G lentiviral vectors delivering transgenes expressing puromycin and shRNA targeting chANP32A or Negative. Puromycin selected cells transfected with pCOM1-firefly minigenome reporter, avian 50-92 polymerase (627E), and Renilla expression control . b, siRNA (100nM) applied to DF-1 chANP32A shRNA cells. After 48hrs, cells transfected with avian 50-92 polymerase (627E), minigenome reporter and Renilla expression control. Luciferase activity measured 20hrs later. Knockdown in chicken DF-1 cells verified by immunoblotting using antibody against vinculin and chANP32A. c, DF-1 cells depleted of chANP32A by siRNA infected with avian-like influenza virus (PR8 virus bearing H5N1 Ty05 polymerase genes with PB2 627E, MOI 0.01). 24hrs later cell supernatants titrated for infectious virus by plaque assay on MDCK cells. d , 293T cells transduced with lentiviral vectors delivering transgenes expressing puromycin and shRNA targeting huANP32A, huANP32B, both huANP32A and B, or Negative. Puromycin selected cells transfected with, pHOM1-firefly minigenome reporter, human-adapted avian 50-92 polymerase (627K), and Renilla expression control.Luciferase activity measured after 20hrs. Knockdown in 293T cells verified immunoblotting using antibody against vinculin, huANP32A and huANP32B. (a,b d, data are firefly activity normalised to Renilla, plotted as % of Negative or Allstars; error as SEM; One-way ANOVA comparisons to ALLStars or Negative, ns= not significant, **p

    Article Snippet: Chicken fibroblast (DF-1) (ATCC) cells were maintained in DF-1 cell culture media (DMEM supplemented with 10% FCS, 5% tryptose phosphate broth (Sigma-Aldrich) and 0.1% Penicillin-streptomycin (Invitrogen)).

    Techniques: Transduction, Expressing, shRNA, Transfection, Luciferase, Activity Assay, Infection, Plaque Assay