trypticase soy broth Search Results


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  • 99
    Thermo Fisher tryptic soy broth tsb
    Schematic representation of the experimental design of this study: (A) Strains fermentation in <t>TSB</t> and <t>SSM,</t> (B) Commercial mix culture fermentation in SSM and SBB.
    Tryptic Soy Broth Tsb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 386 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore trypticase soy broth
    The biofilm formation by S. aureus and S. epidermidis cultivated in Basal medium (BM), Luria-Bertani broth (LB), Müller-Hinton broth (MH), or <t>Trypticase</t> soy broth (TSB) on 35-mm polystyrol adhesive plates. 72 hours-old biofilms were stained by crystal violet.
    Trypticase Soy Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Difco trypticase soy broth
    Comparison of S. aureus ISP479C and ISP479R growth kinetics in vitro. Log-phase organisms were dually inoculated (10 3 CFU of each strain) into 10 ml of BHI broth, and the cultures were incubated on a rotary shaker (for aeration) at 37°C. At selected time points, aliquots were quantitatively cultured on BHI agar with or without erythromycin (10 μg/ml; as described in Materials and Methods). Data points represent the mean values from two independent experiments performed in duplicate. In no case was the deviation from the mean greater than 0.2 log CFU/ml; error bars were intentionally excluded to improve clarity. These results mirrored those of identical dual-culture experiments performed with nutrient or <t>Trypticase</t> soy broth (see Materials and Methods). These results were not significantly different from those for either organism cultured alone (data not shown).
    Trypticase Soy Broth, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 782 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson trypticase soy broth
    Microbicidal kinetics. P. aeruginosa strain 2 was exposed to the indicated concentrations of novispirin G10 in a medium composed of 100 mM NaCl, 10 mM sodium phosphate buffer (pH 7.4), and 0.3 mg of <t>Trypticase</t> soy broth powder/ml. Aliquots were removed after 5 and 10 min, diluted, spread on Trypticase soy agar plates, and incubated overnight to allow colony development.
    Trypticase Soy Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 2031 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher trypticase soy broth
    The bioactive molecules are secreted. Strains of C. citroniae and C. aldenense that showed strong activity against hilA expression were cultured under anaerobic conditions in <t>Trypticase</t> soy broth supplemented with menadione and hemin for at least 2 days, and the cells were separated from the supernatants through centrifugation. Both the cells and the supernatants were then extracted with ethyl acetate, and dried extracts were added to LB broth, which was used to culture the Salmonella hilA :: gfp reporter strain for measurements of hilA expression. Results shown are the averages of 3 individual measurements, and bars show the standard errors of the means. ***, P
    Trypticase Soy Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 594 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Becton Dickinson tryptic soy broth tsb
    Specific activity of Caa. Growth of complementation strain in <t>TSB</t> and minimal media with fructose. A) Specific activity of purified Strep2-tagged CA enzymes. Substrates used for this assay were bicarbonate (50 mM, dark bars) and CO 2 (saturated solution, grey bars). CA activity was measured by comparing the rapid change of pH in the presence of CA to the slower pH change in the absence of CA. The pH change was monitored using phenol red and the absorbance A 557nm was measured for 60 sec. Average values from three experiments were plotted with error bars representing the standard deviation. B) Cultures of R. <t>eutropha</t> wild type (H16 - ●), Re2428 (○), Re2428/pCaa (▼), and Re2428pCaaB (∆) in TSB media. C) Growth in minimal media with 2% fructose (w vol -1 ). Values represent average from three replicates with standard deviation as error bars.
    Tryptic Soy Broth Tsb, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 702 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Difco tryptic soy broth tsb
    Fisetin at the concentrations of 32 μg/ml does not affect the growth characteristics of <t>SC19.</t> (A) SC19 was co-incubated with 0, 8, 16, 32, 64, 128 μg/ml fisetin in <t>TSB</t> containing 10% of newborn bovine serum, and the growth characteristic of SC19 was measured by OD 600 every hour. (B) The effect of fisetin (0, 8, 16, 32 μg/ml) on the expression of suilysin in SC19 supernatant was investigated at 0, 4, 8, and 16 h by western blotting. Exogenous addition of BSA was detected as an internal control using an anti-BSA antibody. The medium supernatant without addition of SC19 was detected by western blot assay at 0 h.
    Tryptic Soy Broth Tsb, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 900 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA tryptic soy broth
    Fisetin at the concentrations of 32 μg/ml does not affect the growth characteristics of <t>SC19.</t> (A) SC19 was co-incubated with 0, 8, 16, 32, 64, 128 μg/ml fisetin in <t>TSB</t> containing 10% of newborn bovine serum, and the growth characteristic of SC19 was measured by OD 600 every hour. (B) The effect of fisetin (0, 8, 16, 32 μg/ml) on the expression of suilysin in SC19 supernatant was investigated at 0, 4, 8, and 16 h by western blotting. Exogenous addition of BSA was detected as an internal control using an anti-BSA antibody. The medium supernatant without addition of SC19 was detected by western blot assay at 0 h.
    Tryptic Soy Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co tryptic soy broth
    Fisetin at the concentrations of 32 μg/ml does not affect the growth characteristics of <t>SC19.</t> (A) SC19 was co-incubated with 0, 8, 16, 32, 64, 128 μg/ml fisetin in <t>TSB</t> containing 10% of newborn bovine serum, and the growth characteristic of SC19 was measured by OD 600 every hour. (B) The effect of fisetin (0, 8, 16, 32 μg/ml) on the expression of suilysin in SC19 supernatant was investigated at 0, 4, 8, and 16 h by western blotting. Exogenous addition of BSA was detected as an internal control using an anti-BSA antibody. The medium supernatant without addition of SC19 was detected by western blot assay at 0 h.
    Tryptic Soy Broth, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson trypticase soy agar tsa
    Fisetin at the concentrations of 32 μg/ml does not affect the growth characteristics of <t>SC19.</t> (A) SC19 was co-incubated with 0, 8, 16, 32, 64, 128 μg/ml fisetin in <t>TSB</t> containing 10% of newborn bovine serum, and the growth characteristic of SC19 was measured by OD 600 every hour. (B) The effect of fisetin (0, 8, 16, 32 μg/ml) on the expression of suilysin in SC19 supernatant was investigated at 0, 4, 8, and 16 h by western blotting. Exogenous addition of BSA was detected as an internal control using an anti-BSA antibody. The medium supernatant without addition of SC19 was detected by western blot assay at 0 h.
    Trypticase Soy Agar Tsa, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA tryptic soy broth tsb
    Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas <t>brassicacearum</t> MA250 pure culture in <t>TSB,</t> 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each
    Tryptic Soy Broth Tsb, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Becton Dickinson bacto tryptic soy broth
    Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas <t>brassicacearum</t> MA250 pure culture in <t>TSB,</t> 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each
    Bacto Tryptic Soy Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Becton Dickinson trypticase soy agar plates
    Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas <t>brassicacearum</t> MA250 pure culture in <t>TSB,</t> 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each
    Trypticase Soy Agar Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    HiMedia Laboratories trypticase soy broth
    Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas <t>brassicacearum</t> MA250 pure culture in <t>TSB,</t> 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each
    Trypticase Soy Broth, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Difco trypticase soy agar tsa
    Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas <t>brassicacearum</t> MA250 pure culture in <t>TSB,</t> 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each
    Trypticase Soy Agar Tsa, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Liofilchem tryptic soy broth
    Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas <t>brassicacearum</t> MA250 pure culture in <t>TSB,</t> 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each
    Tryptic Soy Broth, supplied by Liofilchem, used in various techniques. Bioz Stars score: 92/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avantor trypticase soy broth
    Comparison of antibiotic maximal efficacies ( E max ) expressed as the reduction in the number of CFU from that for the control (left) or as a percentage of the reduction in metabolic activity (resorufin fluorescence; middle) or biofilm mass (crystal violet [CV] absorbance; right) compared to that for an untreated biofilm for strain ATCC 25923 (open bar) or ATCC 33591 (closed bars) grown in <t>Trypticase</t> soy broth supplemented with 1% glucose and 2% NaCl (TGN) or in artificial sputum medium (ASM). MEM, meropenem; VAN, vancomycin; LZD, linezolid; AZM, azithromycin; RIF, rifampin; CIP, ciprofloxacin; TOB, tobramycin. Values are means ± SEM. Statistical analyses were by one-way analysis of variance (ANOVA) with Tukey’s posttest for multiple comparisons; values with different letters are significantly different from each other ( P  
    Trypticase Soy Broth, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Becton Dickinson trypticase peptone
    Most-probable-numbers (MPN) counts of <t>Trypticase</t> and amino acid-utilising bacteria in faeces from human omnivorous (O2 and O3) and vegetarian (V1 and V2) donors. Results are from 7-d counts. Error bars represent 95% confidence levels.
    Trypticase Peptone, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Difco tryptic soy broth medium
    Most-probable-numbers (MPN) counts of <t>Trypticase</t> and amino acid-utilising bacteria in faeces from human omnivorous (O2 and O3) and vegetarian (V1 and V2) donors. Results are from 7-d counts. Error bars represent 95% confidence levels.
    Tryptic Soy Broth Medium, supplied by Difco, used in various techniques. Bioz Stars score: 91/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Schematic representation of the experimental design of this study: (A) Strains fermentation in TSB and SSM, (B) Commercial mix culture fermentation in SSM and SBB.

    Journal: Foods

    Article Title: Arthrospira platensis as Natural Fermentation Booster for Milk and Soy Fermented Beverages

    doi: 10.3390/foods9030350

    Figure Lengend Snippet: Schematic representation of the experimental design of this study: (A) Strains fermentation in TSB and SSM, (B) Commercial mix culture fermentation in SSM and SBB.

    Article Snippet: Three different growth substrates were considered: Tryptic Soy Broth (TSB) (Oxoid), reconstituted skim milk powder (SSM) (Oxoid, Ltd., Basingstoke, United Kingdom), and a commercial soy-based beverage (SBB).

    Techniques:

    The biofilm formation by S. aureus and S. epidermidis cultivated in Basal medium (BM), Luria-Bertani broth (LB), Müller-Hinton broth (MH), or Trypticase soy broth (TSB) on 35-mm polystyrol adhesive plates. 72 hours-old biofilms were stained by crystal violet.

    Journal: Scientific Reports

    Article Title: Targeting microbial biofilms using Ficin, a nonspecific plant protease

    doi: 10.1038/srep46068

    Figure Lengend Snippet: The biofilm formation by S. aureus and S. epidermidis cultivated in Basal medium (BM), Luria-Bertani broth (LB), Müller-Hinton broth (MH), or Trypticase soy broth (TSB) on 35-mm polystyrol adhesive plates. 72 hours-old biofilms were stained by crystal violet.

    Article Snippet: The Müller-Hinton broth (Fluka) or Trypticase soy broth (Sigma) did not provide stable biofilm formation by both Staphylococcus aureus and Staphylococcus epidermidis , as is has been determined in preliminary studies , thus the modified Basal medium (BM) (glucose 5 g, peptone 7 g, MgSO4 × 7H2 O 2.0 g and CaCl2 × 2H2 O 0.05 g in 1.0 liter tap water) was used for the biofilm formation assays .

    Techniques: Staining

    Adherence of S. aureus Newman complemented mutants grown in TSB and iron restricted conditions to desquamated nasal epithelial cells . The ability of mutants of strain Newman carrying complementing pCU1 plasmids carrying surface protein genes to adhere to desquamated nasal epithelial cells was tested. Strains Newman clfA , Newman clfA isdA clfB sdrCDE , Newman clfA isdA clfB sdrCDE (pCU1), Newman clfA isdA clfB sdrCDE (pCU1 clfB + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrC + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrD + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrE + ),) Newman clfA isdA clfB sdrCDE (pCU1 isdAB + ) and Newman clfA isdA clfB sdrCDE (pCU1 isdB + ) grown to the exponential phase in (A) TSB and to the stationary phase in (B) RPMI were tested for adherence. Counts represent the number of bacterial cells adhering to 100 squamous cells. Results are expressed as the mean of triplicate experiments +/- standard deviations.

    Journal: BMC Microbiology

    Article Title: Surface proteins that promote adherence of Staphylococcus aureus to human desquamated nasal epithelial cells

    doi: 10.1186/1471-2180-9-22

    Figure Lengend Snippet: Adherence of S. aureus Newman complemented mutants grown in TSB and iron restricted conditions to desquamated nasal epithelial cells . The ability of mutants of strain Newman carrying complementing pCU1 plasmids carrying surface protein genes to adhere to desquamated nasal epithelial cells was tested. Strains Newman clfA , Newman clfA isdA clfB sdrCDE , Newman clfA isdA clfB sdrCDE (pCU1), Newman clfA isdA clfB sdrCDE (pCU1 clfB + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrC + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrD + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrE + ),) Newman clfA isdA clfB sdrCDE (pCU1 isdAB + ) and Newman clfA isdA clfB sdrCDE (pCU1 isdB + ) grown to the exponential phase in (A) TSB and to the stationary phase in (B) RPMI were tested for adherence. Counts represent the number of bacterial cells adhering to 100 squamous cells. Results are expressed as the mean of triplicate experiments +/- standard deviations.

    Article Snippet: S. aureus strains were grown on tryptic soy agar (TSA; Oxoid), tryptic soy broth (TSB) or RPMI 1640 (Sigma).

    Techniques:

    Adherence of Newman mutants to desquamated nasal epithelial cells . The ability of (A) Newman clfA , Newman clfA clfB , Newman clfA sdrCDE and Newman clfA clfB sdrCDE grown to exponential phase in TSB and (B) Newman, Newman clfA , Newman clfA clfB , Newman clfA sdrCDE , Newman clfA isdA , Newman clfA isdA sdrCDE , Newman clfA clfB sdrCDE , Newman clfA isdA clfB , and Newman clfA isdA clfB sdrCDE grown to stationary phase in RPMI to adhere to desquamated human nasal epithelial cells was measured. The tenth track is a control without S. aureus showing background due to adherent bacteria from the donor. Counts represent the number of bacterial cells adhering to 100 squamous cells. Results are expressed as the mean of triplicate experiments +/- standard deviations.

    Journal: BMC Microbiology

    Article Title: Surface proteins that promote adherence of Staphylococcus aureus to human desquamated nasal epithelial cells

    doi: 10.1186/1471-2180-9-22

    Figure Lengend Snippet: Adherence of Newman mutants to desquamated nasal epithelial cells . The ability of (A) Newman clfA , Newman clfA clfB , Newman clfA sdrCDE and Newman clfA clfB sdrCDE grown to exponential phase in TSB and (B) Newman, Newman clfA , Newman clfA clfB , Newman clfA sdrCDE , Newman clfA isdA , Newman clfA isdA sdrCDE , Newman clfA clfB sdrCDE , Newman clfA isdA clfB , and Newman clfA isdA clfB sdrCDE grown to stationary phase in RPMI to adhere to desquamated human nasal epithelial cells was measured. The tenth track is a control without S. aureus showing background due to adherent bacteria from the donor. Counts represent the number of bacterial cells adhering to 100 squamous cells. Results are expressed as the mean of triplicate experiments +/- standard deviations.

    Article Snippet: S. aureus strains were grown on tryptic soy agar (TSA; Oxoid), tryptic soy broth (TSB) or RPMI 1640 (Sigma).

    Techniques:

    Comparison of S. aureus ISP479C and ISP479R growth kinetics in vitro. Log-phase organisms were dually inoculated (10 3 CFU of each strain) into 10 ml of BHI broth, and the cultures were incubated on a rotary shaker (for aeration) at 37°C. At selected time points, aliquots were quantitatively cultured on BHI agar with or without erythromycin (10 μg/ml; as described in Materials and Methods). Data points represent the mean values from two independent experiments performed in duplicate. In no case was the deviation from the mean greater than 0.2 log CFU/ml; error bars were intentionally excluded to improve clarity. These results mirrored those of identical dual-culture experiments performed with nutrient or Trypticase soy broth (see Materials and Methods). These results were not significantly different from those for either organism cultured alone (data not shown).

    Journal: Infection and Immunity

    Article Title: In Vitro Resistance to Thrombin-Induced Platelet Microbicidal Protein Is Associated with Enhanced Progression and Hematogenous Dissemination in Experimental Staphylococcus aureus Infective Endocarditis

    doi:

    Figure Lengend Snippet: Comparison of S. aureus ISP479C and ISP479R growth kinetics in vitro. Log-phase organisms were dually inoculated (10 3 CFU of each strain) into 10 ml of BHI broth, and the cultures were incubated on a rotary shaker (for aeration) at 37°C. At selected time points, aliquots were quantitatively cultured on BHI agar with or without erythromycin (10 μg/ml; as described in Materials and Methods). Data points represent the mean values from two independent experiments performed in duplicate. In no case was the deviation from the mean greater than 0.2 log CFU/ml; error bars were intentionally excluded to improve clarity. These results mirrored those of identical dual-culture experiments performed with nutrient or Trypticase soy broth (see Materials and Methods). These results were not significantly different from those for either organism cultured alone (data not shown).

    Article Snippet: For these studies, organisms were inoculated (∼103 CFU/ml) into brain heart infusion (BHI) broth, nutrient broth, or Trypticase soy broth (all media were from Difco Laboratories, Detroit, Mich.) and monitored for CFU/ml at selected times ranging from 1 to 24 h, with constant rotary shaking at 37°C.

    Techniques: In Vitro, Incubation, Cell Culture

    Microbicidal kinetics. P. aeruginosa strain 2 was exposed to the indicated concentrations of novispirin G10 in a medium composed of 100 mM NaCl, 10 mM sodium phosphate buffer (pH 7.4), and 0.3 mg of Trypticase soy broth powder/ml. Aliquots were removed after 5 and 10 min, diluted, spread on Trypticase soy agar plates, and incubated overnight to allow colony development.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns

    doi: 10.1128/AAC.46.6.1837-1844.2002

    Figure Lengend Snippet: Microbicidal kinetics. P. aeruginosa strain 2 was exposed to the indicated concentrations of novispirin G10 in a medium composed of 100 mM NaCl, 10 mM sodium phosphate buffer (pH 7.4), and 0.3 mg of Trypticase soy broth powder/ml. Aliquots were removed after 5 and 10 min, diluted, spread on Trypticase soy agar plates, and incubated overnight to allow colony development.

    Article Snippet: The overlay agar consisted of 6% (wt/vol) Trypticase soy broth and 1% agarose in phosphate-buffered saline for all assays.

    Techniques: Incubation

    Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .

    Journal: Journal of Burn Care & Research: Official Publication of the American Burn Association

    Article Title: Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model

    doi: 10.1093/jbcr/iry043

    Figure Lengend Snippet: Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .

    Article Snippet: The samples were serial diluted with PBS and plated on Trypticase soy agar containing 5% sheep’s blood (Becton, Dickinson and Co.) as well as P. aeruginosa isolation agar (Hardy Diagnostics, Santa Maria, CA) using a WASP 2 Spiral Plater (Microbiology International, Frederick, MD).

    Techniques: Infection, Serial Dilution, Isolation

    The bioactive molecules are secreted. Strains of C. citroniae and C. aldenense that showed strong activity against hilA expression were cultured under anaerobic conditions in Trypticase soy broth supplemented with menadione and hemin for at least 2 days, and the cells were separated from the supernatants through centrifugation. Both the cells and the supernatants were then extracted with ethyl acetate, and dried extracts were added to LB broth, which was used to culture the Salmonella hilA :: gfp reporter strain for measurements of hilA expression. Results shown are the averages of 3 individual measurements, and bars show the standard errors of the means. ***, P

    Journal: mBio

    Article Title: Antivirulence Activity of the Human Gut Metabolome

    doi: 10.1128/mBio.01183-14

    Figure Lengend Snippet: The bioactive molecules are secreted. Strains of C. citroniae and C. aldenense that showed strong activity against hilA expression were cultured under anaerobic conditions in Trypticase soy broth supplemented with menadione and hemin for at least 2 days, and the cells were separated from the supernatants through centrifugation. Both the cells and the supernatants were then extracted with ethyl acetate, and dried extracts were added to LB broth, which was used to culture the Salmonella hilA :: gfp reporter strain for measurements of hilA expression. Results shown are the averages of 3 individual measurements, and bars show the standard errors of the means. ***, P

    Article Snippet: Where indicated, intestinal commensals were cultured under anaerobic conditions in Trypticase soy broth (Oxoid, Cambridge, England) supplemented with menadione (1 µg/ml; Sigma-Aldrich) and hemin (5 µg/ml; BDH, Radnor, PA) for at least 2 days.

    Techniques: Activity Assay, Expressing, Cell Culture, Centrifugation

    A human isolate of Clostridium citroniae produces strong activity against Salmonella invasion gene expression. Microbial isolates from a chemostat culture showing activity against hilA were tested individually for biological activity. Isolates were cultured under anaerobic conditions in Trypticase soy broth supplemented with menadione and hemin for at least 2 days, and the cultures were extracted with ethyl acetate. Dried extracts were added to LB broth, which was used to culture a Salmonella hilA :: gfp reporter strain. GFP production was then monitored through flow cytometry. Results shown are the averages of three individual measurements ( n = 3), except for the control culture, where six cultures were used ( n = 6). Bars indicate the standard errors of the means.

    Journal: mBio

    Article Title: Antivirulence Activity of the Human Gut Metabolome

    doi: 10.1128/mBio.01183-14

    Figure Lengend Snippet: A human isolate of Clostridium citroniae produces strong activity against Salmonella invasion gene expression. Microbial isolates from a chemostat culture showing activity against hilA were tested individually for biological activity. Isolates were cultured under anaerobic conditions in Trypticase soy broth supplemented with menadione and hemin for at least 2 days, and the cultures were extracted with ethyl acetate. Dried extracts were added to LB broth, which was used to culture a Salmonella hilA :: gfp reporter strain. GFP production was then monitored through flow cytometry. Results shown are the averages of three individual measurements ( n = 3), except for the control culture, where six cultures were used ( n = 6). Bars indicate the standard errors of the means.

    Article Snippet: Where indicated, intestinal commensals were cultured under anaerobic conditions in Trypticase soy broth (Oxoid, Cambridge, England) supplemented with menadione (1 µg/ml; Sigma-Aldrich) and hemin (5 µg/ml; BDH, Radnor, PA) for at least 2 days.

    Techniques: Activity Assay, Expressing, Cell Culture, Flow Cytometry, Cytometry

    Closely related Clostridium species are involved in the production of the inhibitory molecules. (A) Strains from diverse Clostridium species were tested for biological activity against hilA . Strains were cultured under anaerobic conditions in Trypticase soy broth supplemented with menadione and hemin for at least 2 days, and the cultures were extracted with ethyl acetate. Dried extracts were added to LB broth, which was used to culture a Salmonella hilA :: gfp reporter strain. GFP production was then monitored through flow cytometry. Results shown are the averages of three individual measurements ( n = 3), except for the control culture, where six cultures were used ( n = 6). Bars indicate the standard errors of the means. (B) Production of the inhibitory molecule by select Clostridium strains was confirmed through RT-PCR targeting hilA . Results shown are the averages of 2 to 6 individual measurements, and bars show the standard errors of the means. *, P

    Journal: mBio

    Article Title: Antivirulence Activity of the Human Gut Metabolome

    doi: 10.1128/mBio.01183-14

    Figure Lengend Snippet: Closely related Clostridium species are involved in the production of the inhibitory molecules. (A) Strains from diverse Clostridium species were tested for biological activity against hilA . Strains were cultured under anaerobic conditions in Trypticase soy broth supplemented with menadione and hemin for at least 2 days, and the cultures were extracted with ethyl acetate. Dried extracts were added to LB broth, which was used to culture a Salmonella hilA :: gfp reporter strain. GFP production was then monitored through flow cytometry. Results shown are the averages of three individual measurements ( n = 3), except for the control culture, where six cultures were used ( n = 6). Bars indicate the standard errors of the means. (B) Production of the inhibitory molecule by select Clostridium strains was confirmed through RT-PCR targeting hilA . Results shown are the averages of 2 to 6 individual measurements, and bars show the standard errors of the means. *, P

    Article Snippet: Where indicated, intestinal commensals were cultured under anaerobic conditions in Trypticase soy broth (Oxoid, Cambridge, England) supplemented with menadione (1 µg/ml; Sigma-Aldrich) and hemin (5 µg/ml; BDH, Radnor, PA) for at least 2 days.

    Techniques: Activity Assay, Cell Culture, Flow Cytometry, Cytometry, Reverse Transcription Polymerase Chain Reaction

    Specific activity of Caa. Growth of complementation strain in TSB and minimal media with fructose. A) Specific activity of purified Strep2-tagged CA enzymes. Substrates used for this assay were bicarbonate (50 mM, dark bars) and CO 2 (saturated solution, grey bars). CA activity was measured by comparing the rapid change of pH in the presence of CA to the slower pH change in the absence of CA. The pH change was monitored using phenol red and the absorbance A 557nm was measured for 60 sec. Average values from three experiments were plotted with error bars representing the standard deviation. B) Cultures of R. eutropha wild type (H16 - ●), Re2428 (○), Re2428/pCaa (▼), and Re2428pCaaB (∆) in TSB media. C) Growth in minimal media with 2% fructose (w vol -1 ). Values represent average from three replicates with standard deviation as error bars.

    Journal: AMB Express

    Article Title: Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16

    doi: 10.1186/2191-0855-4-2

    Figure Lengend Snippet: Specific activity of Caa. Growth of complementation strain in TSB and minimal media with fructose. A) Specific activity of purified Strep2-tagged CA enzymes. Substrates used for this assay were bicarbonate (50 mM, dark bars) and CO 2 (saturated solution, grey bars). CA activity was measured by comparing the rapid change of pH in the presence of CA to the slower pH change in the absence of CA. The pH change was monitored using phenol red and the absorbance A 557nm was measured for 60 sec. Average values from three experiments were plotted with error bars representing the standard deviation. B) Cultures of R. eutropha wild type (H16 - ●), Re2428 (○), Re2428/pCaa (▼), and Re2428pCaaB (∆) in TSB media. C) Growth in minimal media with 2% fructose (w vol -1 ). Values represent average from three replicates with standard deviation as error bars.

    Article Snippet: Growth media and cultivation conditions R. eutropha strains were propagated in tryptic soy broth (TSB) (Becton Dickinson, Sparks, MD) or minimal medium [ ] with fructose at a final concentration of 1% or 2% (w vol-1 ), or pyruvate, lactate, succinate, or formate, each at a final concentration of 0.2% (w vol-1 ) or (vol vol-1 ).

    Techniques: Activity Assay, Cellular Antioxidant Activity Assay, Purification, Size-exclusion Chromatography, Standard Deviation

    Growth of the quadruple deletion mutant on TSB + 10% CO 2 . Cultures of R. eutropha H16 (□), R. eutropha CA quadruple deletion mutant Re2436 (H16 ∆ can ∆ can2 ∆ caa ∆ cag - ●) and the complemented strains, Re2436/pCan (○), Re2436/pCan2 (▼), Re2436/Caa (∆) and Re2436/pCag (■) in TSB media in the presence of 10% CO 2 . Values represent average from three replicates with standard deviation as error bars.

    Journal: AMB Express

    Article Title: Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16

    doi: 10.1186/2191-0855-4-2

    Figure Lengend Snippet: Growth of the quadruple deletion mutant on TSB + 10% CO 2 . Cultures of R. eutropha H16 (□), R. eutropha CA quadruple deletion mutant Re2436 (H16 ∆ can ∆ can2 ∆ caa ∆ cag - ●) and the complemented strains, Re2436/pCan (○), Re2436/pCan2 (▼), Re2436/Caa (∆) and Re2436/pCag (■) in TSB media in the presence of 10% CO 2 . Values represent average from three replicates with standard deviation as error bars.

    Article Snippet: Growth media and cultivation conditions R. eutropha strains were propagated in tryptic soy broth (TSB) (Becton Dickinson, Sparks, MD) or minimal medium [ ] with fructose at a final concentration of 1% or 2% (w vol-1 ), or pyruvate, lactate, succinate, or formate, each at a final concentration of 0.2% (w vol-1 ) or (vol vol-1 ).

    Techniques: Mutagenesis, Cellular Antioxidant Activity Assay, Standard Deviation

    Fisetin at the concentrations of 32 μg/ml does not affect the growth characteristics of SC19. (A) SC19 was co-incubated with 0, 8, 16, 32, 64, 128 μg/ml fisetin in TSB containing 10% of newborn bovine serum, and the growth characteristic of SC19 was measured by OD 600 every hour. (B) The effect of fisetin (0, 8, 16, 32 μg/ml) on the expression of suilysin in SC19 supernatant was investigated at 0, 4, 8, and 16 h by western blotting. Exogenous addition of BSA was detected as an internal control using an anti-BSA antibody. The medium supernatant without addition of SC19 was detected by western blot assay at 0 h.

    Journal: Frontiers in Microbiology

    Article Title: Fisetin Lowers Streptococcus suis serotype 2 Pathogenicity in Mice by Inhibiting the Hemolytic Activity of Suilysin

    doi: 10.3389/fmicb.2018.01723

    Figure Lengend Snippet: Fisetin at the concentrations of 32 μg/ml does not affect the growth characteristics of SC19. (A) SC19 was co-incubated with 0, 8, 16, 32, 64, 128 μg/ml fisetin in TSB containing 10% of newborn bovine serum, and the growth characteristic of SC19 was measured by OD 600 every hour. (B) The effect of fisetin (0, 8, 16, 32 μg/ml) on the expression of suilysin in SC19 supernatant was investigated at 0, 4, 8, and 16 h by western blotting. Exogenous addition of BSA was detected as an internal control using an anti-BSA antibody. The medium supernatant without addition of SC19 was detected by western blot assay at 0 h.

    Article Snippet: SC19 and Δsly were grown in tryptic soy broth (TSB) or plated on tryptic soy agar (TSA) (Difco Laboratories, Detroit, MI, United States) with 10% newborn bovine serum added (Sijiqing Biological Engineering Materials Co., Ltd., Hangzhou, China) at 37°C.

    Techniques: Incubation, Expressing, Western Blot

    Inhibition of suilysin-induced hemolysis by fisetin: (A) The culture supernatant of SC19 cultures causes sheep erythrocyte hemolysis. Hemolysis was measured by hemoglobin release at OD 543 . (B) Chemical structure of fisetin. (C) After SC19 cultures were incubated with fisetin (0, 2, 4, 8, 16, and 32 μg/ml) at 37°C for 18 h, 125 μl culture supernatant was incubated with 875 μl of PBS containing a 2% defibrinated sheep blood at 37°C for 30 min. Fisetin significantly inhibited the hemolytic activity of the supernatant of SC19 cultured in TSB with 10% newborn bovine serum with 32 μg/ml fisetin. (D) After SC19 was cultured at 37°C for 18 h, 125 μl culture supernatant incubated with fisetin (0, 2, 4, 8, 16, and 32 μg/ml) at 37°C for 30 min was incubated with 875 μl of PBS containing a 2% defibrinated sheep blood at 37°C for 30 min. The inhibitor effect of fisetin on the suilysin in the supernatant of SC19 is dose dependent. Statistical analyses were performed using the two-tailed unpaired t -test. Statistically significant differences are indicated. ∗ P

    Journal: Frontiers in Microbiology

    Article Title: Fisetin Lowers Streptococcus suis serotype 2 Pathogenicity in Mice by Inhibiting the Hemolytic Activity of Suilysin

    doi: 10.3389/fmicb.2018.01723

    Figure Lengend Snippet: Inhibition of suilysin-induced hemolysis by fisetin: (A) The culture supernatant of SC19 cultures causes sheep erythrocyte hemolysis. Hemolysis was measured by hemoglobin release at OD 543 . (B) Chemical structure of fisetin. (C) After SC19 cultures were incubated with fisetin (0, 2, 4, 8, 16, and 32 μg/ml) at 37°C for 18 h, 125 μl culture supernatant was incubated with 875 μl of PBS containing a 2% defibrinated sheep blood at 37°C for 30 min. Fisetin significantly inhibited the hemolytic activity of the supernatant of SC19 cultured in TSB with 10% newborn bovine serum with 32 μg/ml fisetin. (D) After SC19 was cultured at 37°C for 18 h, 125 μl culture supernatant incubated with fisetin (0, 2, 4, 8, 16, and 32 μg/ml) at 37°C for 30 min was incubated with 875 μl of PBS containing a 2% defibrinated sheep blood at 37°C for 30 min. The inhibitor effect of fisetin on the suilysin in the supernatant of SC19 is dose dependent. Statistical analyses were performed using the two-tailed unpaired t -test. Statistically significant differences are indicated. ∗ P

    Article Snippet: SC19 and Δsly were grown in tryptic soy broth (TSB) or plated on tryptic soy agar (TSA) (Difco Laboratories, Detroit, MI, United States) with 10% newborn bovine serum added (Sijiqing Biological Engineering Materials Co., Ltd., Hangzhou, China) at 37°C.

    Techniques: Inhibition, Incubation, Activity Assay, Cell Culture, Two Tailed Test

    Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas brassicacearum MA250 pure culture in TSB, 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each

    Journal: BMC Microbiology

    Article Title: Isothermal microcalorimetry for thermal viable count of microorganisms in pure cultures and stabilized formulations

    doi: 10.1186/s12866-019-1432-8

    Figure Lengend Snippet: Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas brassicacearum MA250 pure culture in TSB, 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each

    Article Snippet: P. brassicacearum MA250 cells were cultured in tryptic soy broth (TSB) (105,459, Merck, Germany) with shaking at 210 rpm or on TSA (TSB + agar) (105,458, Merck, Germany) at 25 °C.

    Techniques:

    Isothermal microcalorimetry thermal power (μW) curves of microbes in different culturing volumes (3, 6, 12 and 18 ml). a Pseudomonas brassicacearum MA250 in TSB, 25 °C. b Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. c Clonostachys rosea IK726 in PDB, 25 °C. The figures for Pseudomonas and Bacillus represent the averages and standard deviations of three independent experiments with 3–6 replicates per treatment. The figure for C. rosea IK726 represents the averages and standard deviations of six replicates per treatment

    Journal: BMC Microbiology

    Article Title: Isothermal microcalorimetry for thermal viable count of microorganisms in pure cultures and stabilized formulations

    doi: 10.1186/s12866-019-1432-8

    Figure Lengend Snippet: Isothermal microcalorimetry thermal power (μW) curves of microbes in different culturing volumes (3, 6, 12 and 18 ml). a Pseudomonas brassicacearum MA250 in TSB, 25 °C. b Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. c Clonostachys rosea IK726 in PDB, 25 °C. The figures for Pseudomonas and Bacillus represent the averages and standard deviations of three independent experiments with 3–6 replicates per treatment. The figure for C. rosea IK726 represents the averages and standard deviations of six replicates per treatment

    Article Snippet: P. brassicacearum MA250 cells were cultured in tryptic soy broth (TSB) (105,459, Merck, Germany) with shaking at 210 rpm or on TSA (TSB + agar) (105,458, Merck, Germany) at 25 °C.

    Techniques:

    Comparison of antibiotic maximal efficacies ( E max ) expressed as the reduction in the number of CFU from that for the control (left) or as a percentage of the reduction in metabolic activity (resorufin fluorescence; middle) or biofilm mass (crystal violet [CV] absorbance; right) compared to that for an untreated biofilm for strain ATCC 25923 (open bar) or ATCC 33591 (closed bars) grown in Trypticase soy broth supplemented with 1% glucose and 2% NaCl (TGN) or in artificial sputum medium (ASM). MEM, meropenem; VAN, vancomycin; LZD, linezolid; AZM, azithromycin; RIF, rifampin; CIP, ciprofloxacin; TOB, tobramycin. Values are means ± SEM. Statistical analyses were by one-way analysis of variance (ANOVA) with Tukey’s posttest for multiple comparisons; values with different letters are significantly different from each other ( P  

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Activity of Antibiotics against Staphylococcus aureus in an In Vitro Model of Biofilms in the Context of Cystic Fibrosis: Influence of the Culture Medium

    doi: 10.1128/AAC.00602-19

    Figure Lengend Snippet: Comparison of antibiotic maximal efficacies ( E max ) expressed as the reduction in the number of CFU from that for the control (left) or as a percentage of the reduction in metabolic activity (resorufin fluorescence; middle) or biofilm mass (crystal violet [CV] absorbance; right) compared to that for an untreated biofilm for strain ATCC 25923 (open bar) or ATCC 33591 (closed bars) grown in Trypticase soy broth supplemented with 1% glucose and 2% NaCl (TGN) or in artificial sputum medium (ASM). MEM, meropenem; VAN, vancomycin; LZD, linezolid; AZM, azithromycin; RIF, rifampin; CIP, ciprofloxacin; TOB, tobramycin. Values are means ± SEM. Statistical analyses were by one-way analysis of variance (ANOVA) with Tukey’s posttest for multiple comparisons; values with different letters are significantly different from each other ( P  

    Article Snippet: Two media were used in parallel, namely, Trypticase soy broth (VWR, Radnor, PA) supplemented with 1% glucose and 2% NaCl (TGN) ( , ) or artificial sputum medium (ASM) (adapted from previous work [ , , ]).

    Techniques: Activity Assay, Fluorescence

    Metabolic activity in planktonic cultures or biofilm cultures grown in Trypticase soy broth supplemented with 1% glucose and 2% NaCl (TGN) or in artificial sputum medium (ASM). (Left) Resorufin fluorescence signal recorded after 30 min of incubation of planktonic bacteria at increasing inocula with 10-mg/liter resazurin. (Right) Resorufin fluorescence signal recorded after 30 min of incubation of a 24-h-old biofilm with 10-mg/liter resazurin. Data are means ± SD for triplicates in a single experiment or means ± SEM from at least 3 independent experiments performed in triplicate. Statistical analyses comparing strains in each individual medium were performed by one-way analysis of variance (ANOVA) with Tukey’s posttest for multiple comparisons; values with different letters are significantly different from each other ( P  

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Activity of Antibiotics against Staphylococcus aureus in an In Vitro Model of Biofilms in the Context of Cystic Fibrosis: Influence of the Culture Medium

    doi: 10.1128/AAC.00602-19

    Figure Lengend Snippet: Metabolic activity in planktonic cultures or biofilm cultures grown in Trypticase soy broth supplemented with 1% glucose and 2% NaCl (TGN) or in artificial sputum medium (ASM). (Left) Resorufin fluorescence signal recorded after 30 min of incubation of planktonic bacteria at increasing inocula with 10-mg/liter resazurin. (Right) Resorufin fluorescence signal recorded after 30 min of incubation of a 24-h-old biofilm with 10-mg/liter resazurin. Data are means ± SD for triplicates in a single experiment or means ± SEM from at least 3 independent experiments performed in triplicate. Statistical analyses comparing strains in each individual medium were performed by one-way analysis of variance (ANOVA) with Tukey’s posttest for multiple comparisons; values with different letters are significantly different from each other ( P  

    Article Snippet: Two media were used in parallel, namely, Trypticase soy broth (VWR, Radnor, PA) supplemented with 1% glucose and 2% NaCl (TGN) ( , ) or artificial sputum medium (ASM) (adapted from previous work [ , , ]).

    Techniques: Activity Assay, Fluorescence, Incubation

    Most-probable-numbers (MPN) counts of Trypticase and amino acid-utilising bacteria in faeces from human omnivorous (O2 and O3) and vegetarian (V1 and V2) donors. Results are from 7-d counts. Error bars represent 95% confidence levels.

    Journal: BMC Microbiology

    Article Title: Ammonia production by human faecal bacteria, and the enumeration, isolation and characterization of bacteria capable of growth on peptides and amino acids

    doi: 10.1186/1471-2180-13-6

    Figure Lengend Snippet: Most-probable-numbers (MPN) counts of Trypticase and amino acid-utilising bacteria in faeces from human omnivorous (O2 and O3) and vegetarian (V1 and V2) donors. Results are from 7-d counts. Error bars represent 95% confidence levels.

    Article Snippet: These dilutions were used to inoculate (1%, v/v) Hungate tubes containing four different liquid media: A, complete liquid form of medium M2 [ ]; B, basal + 15 g/liter Trypticase® peptone (Becton Dickinson Microbiology Systems, Cockeysville, MD 21030); C, medium B + 5 μM monensin; D, basal + 15 g l-1 Casamino acids (Difco, Becton Dickinson Europe, 38241 Meylan cedex, France).

    Techniques: