Journal: Antimicrobial Agents and Chemotherapy
Article Title: Activity of Antibiotics against Staphylococcus aureus in an In Vitro Model of Biofilms in the Context of Cystic Fibrosis: Influence of the Culture Medium
Figure Lengend Snippet: Comparison of antibiotic maximal efficacies ( E max ) expressed as the reduction in the number of CFU from that for the control (left) or as a percentage of the reduction in metabolic activity (resorufin fluorescence; middle) or biofilm mass (crystal violet [CV] absorbance; right) compared to that for an untreated biofilm for strain ATCC 25923 (open bar) or ATCC 33591 (closed bars) grown in Trypticase soy broth supplemented with 1% glucose and 2% NaCl (TGN) or in artificial sputum medium (ASM). MEM, meropenem; VAN, vancomycin; LZD, linezolid; AZM, azithromycin; RIF, rifampin; CIP, ciprofloxacin; TOB, tobramycin. Values are means ÃÂ± SEM. Statistical analyses were by one-way analysis of variance (ANOVA) with TukeyÃ¢ÂÂs posttest for multiple comparisons; values with different letters are significantly different from each other ( P Ã¢ÂÂ
Article Snippet: Two media were used in parallel, namely, Trypticase soy broth (VWR, Radnor, PA) supplemented with 1% glucose and 2% NaCl (TGN) ( , ) or artificial sputum medium (ASM) (adapted from previous work [ , , ]).
Techniques: Activity Assay, Fluorescence