trypticase soy broth Search Results


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  • 99
    Millipore trypticase soy broth
    The biofilm formation by S. aureus and S. epidermidis cultivated in Basal medium (BM), Luria-Bertani broth (LB), Müller-Hinton broth (MH), or <t>Trypticase</t> soy broth (TSB) on 35-mm polystyrol adhesive plates. 72 hours-old biofilms were stained by crystal violet.
    Trypticase Soy Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Difco trypticase soy broth
    Comparison of S. aureus ISP479C and ISP479R growth kinetics in vitro. Log-phase organisms were dually inoculated (10 3 CFU of each strain) into 10 ml of BHI broth, and the cultures were incubated on a rotary shaker (for aeration) at 37°C. At selected time points, aliquots were quantitatively cultured on BHI agar with or without erythromycin (10 μg/ml; as described in Materials and Methods). Data points represent the mean values from two independent experiments performed in duplicate. In no case was the deviation from the mean greater than 0.2 log CFU/ml; error bars were intentionally excluded to improve clarity. These results mirrored those of identical dual-culture experiments performed with nutrient or <t>Trypticase</t> soy broth (see Materials and Methods). These results were not significantly different from those for either organism cultured alone (data not shown).
    Trypticase Soy Broth, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 704 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bio-Rad trypticase soy broth
    Comparison of S. aureus ISP479C and ISP479R growth kinetics in vitro. Log-phase organisms were dually inoculated (10 3 CFU of each strain) into 10 ml of BHI broth, and the cultures were incubated on a rotary shaker (for aeration) at 37°C. At selected time points, aliquots were quantitatively cultured on BHI agar with or without erythromycin (10 μg/ml; as described in Materials and Methods). Data points represent the mean values from two independent experiments performed in duplicate. In no case was the deviation from the mean greater than 0.2 log CFU/ml; error bars were intentionally excluded to improve clarity. These results mirrored those of identical dual-culture experiments performed with nutrient or <t>Trypticase</t> soy broth (see Materials and Methods). These results were not significantly different from those for either organism cultured alone (data not shown).
    Trypticase Soy Broth, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Avantor trypticase soy broth
    Comparison of antibiotic maximal efficacies ( E max ) expressed as the reduction in the number of CFU from that for the control (left) or as a percentage of the reduction in metabolic activity (resorufin fluorescence; middle) or biofilm mass (crystal violet [CV] absorbance; right) compared to that for an untreated biofilm for strain ATCC 25923 (open bar) or ATCC 33591 (closed bars) grown in <t>Trypticase</t> soy broth supplemented with 1% glucose and 2% NaCl (TGN) or in artificial sputum medium (ASM). MEM, meropenem; VAN, vancomycin; LZD, linezolid; AZM, azithromycin; RIF, rifampin; CIP, ciprofloxacin; TOB, tobramycin. Values are means ± SEM. Statistical analyses were by one-way analysis of variance (ANOVA) with Tukey’s posttest for multiple comparisons; values with different letters are significantly different from each other ( P  
    Trypticase Soy Broth, supplied by Avantor, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson trypticase soy broth
    Microbicidal kinetics. P. aeruginosa strain 2 was exposed to the indicated concentrations of novispirin G10 in a medium composed of 100 mM NaCl, 10 mM sodium phosphate buffer (pH 7.4), and 0.3 mg of <t>Trypticase</t> soy broth powder/ml. Aliquots were removed after 5 and 10 min, diluted, spread on Trypticase soy agar plates, and incubated overnight to allow colony development.
    Trypticase Soy Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 1293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck KGaA tryptic soy broth
    Microbicidal kinetics. P. aeruginosa strain 2 was exposed to the indicated concentrations of novispirin G10 in a medium composed of 100 mM NaCl, 10 mM sodium phosphate buffer (pH 7.4), and 0.3 mg of <t>Trypticase</t> soy broth powder/ml. Aliquots were removed after 5 and 10 min, diluted, spread on Trypticase soy agar plates, and incubated overnight to allow colony development.
    Tryptic Soy Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher trypticase soy broth
    The bioactive molecules are secreted. Strains of C. citroniae and C. aldenense that showed strong activity against hilA expression were cultured under anaerobic conditions in <t>Trypticase</t> soy broth supplemented with menadione and hemin for at least 2 days, and the cells were separated from the supernatants through centrifugation. Both the cells and the supernatants were then extracted with ethyl acetate, and dried extracts were added to LB broth, which was used to culture the Salmonella hilA :: gfp reporter strain for measurements of hilA expression. Results shown are the averages of 3 individual measurements, and bars show the standard errors of the means. ***, P
    Trypticase Soy Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 374 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher soy trypticase broth
    The bioactive molecules are secreted. Strains of C. citroniae and C. aldenense that showed strong activity against hilA expression were cultured under anaerobic conditions in <t>Trypticase</t> soy broth supplemented with menadione and hemin for at least 2 days, and the cells were separated from the supernatants through centrifugation. Both the cells and the supernatants were then extracted with ethyl acetate, and dried extracts were added to LB broth, which was used to culture the Salmonella hilA :: gfp reporter strain for measurements of hilA expression. Results shown are the averages of 3 individual measurements, and bars show the standard errors of the means. ***, P
    Soy Trypticase Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck & Co tryptic soy broth
    The bioactive molecules are secreted. Strains of C. citroniae and C. aldenense that showed strong activity against hilA expression were cultured under anaerobic conditions in <t>Trypticase</t> soy broth supplemented with menadione and hemin for at least 2 days, and the cells were separated from the supernatants through centrifugation. Both the cells and the supernatants were then extracted with ethyl acetate, and dried extracts were added to LB broth, which was used to culture the Salmonella hilA :: gfp reporter strain for measurements of hilA expression. Results shown are the averages of 3 individual measurements, and bars show the standard errors of the means. ***, P
    Tryptic Soy Broth, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    scharlau tryptic soy broth
    The bioactive molecules are secreted. Strains of C. citroniae and C. aldenense that showed strong activity against hilA expression were cultured under anaerobic conditions in <t>Trypticase</t> soy broth supplemented with menadione and hemin for at least 2 days, and the cells were separated from the supernatants through centrifugation. Both the cells and the supernatants were then extracted with ethyl acetate, and dried extracts were added to LB broth, which was used to culture the Salmonella hilA :: gfp reporter strain for measurements of hilA expression. Results shown are the averages of 3 individual measurements, and bars show the standard errors of the means. ***, P
    Tryptic Soy Broth, supplied by scharlau, used in various techniques. Bioz Stars score: 92/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Q Laboratories trypticase soy broth
    The bioactive molecules are secreted. Strains of C. citroniae and C. aldenense that showed strong activity against hilA expression were cultured under anaerobic conditions in <t>Trypticase</t> soy broth supplemented with menadione and hemin for at least 2 days, and the cells were separated from the supernatants through centrifugation. Both the cells and the supernatants were then extracted with ethyl acetate, and dried extracts were added to LB broth, which was used to culture the Salmonella hilA :: gfp reporter strain for measurements of hilA expression. Results shown are the averages of 3 individual measurements, and bars show the standard errors of the means. ***, P
    Trypticase Soy Broth, supplied by Q Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    bioMerieux trypticase soy broth
    Real time measurement of biofilm permeability, after 4 h of phage treatment. ( A ) Membrane coverage analysis by crystal violet (CV) staining and ImageJ imaging software. Native membrane was used as a control. ( B ) Laser interferometry analysis of <t>trypticase-soy</t> broth (TSB) medium diffusion through PAO1 biofilm treated with PA5oct phage. Untreated biofilm was used as a control. Error bars denote SD. The results displayed are the mean of three independent experiments. Statistical analysis was made by the ANOVA test to compare data of treated biofilm versus control native biofilm at 40 min time point. The examples of interferograms (40 min) for PAO1 biofilm treated with active and inactivated PA5oct phages as well as control (from the top of a right-hand panel).
    Trypticase Soy Broth, supplied by bioMerieux, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    DSMZ trypticase soy broth
    Real time measurement of biofilm permeability, after 4 h of phage treatment. ( A ) Membrane coverage analysis by crystal violet (CV) staining and ImageJ imaging software. Native membrane was used as a control. ( B ) Laser interferometry analysis of <t>trypticase-soy</t> broth (TSB) medium diffusion through PAO1 biofilm treated with PA5oct phage. Untreated biofilm was used as a control. Error bars denote SD. The results displayed are the mean of three independent experiments. Statistical analysis was made by the ANOVA test to compare data of treated biofilm versus control native biofilm at 40 min time point. The examples of interferograms (40 min) for PAO1 biofilm treated with active and inactivated PA5oct phages as well as control (from the top of a right-hand panel).
    Trypticase Soy Broth, supplied by DSMZ, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Nissui Pharmaceutical trypticase soy broth
    Real time measurement of biofilm permeability, after 4 h of phage treatment. ( A ) Membrane coverage analysis by crystal violet (CV) staining and ImageJ imaging software. Native membrane was used as a control. ( B ) Laser interferometry analysis of <t>trypticase-soy</t> broth (TSB) medium diffusion through PAO1 biofilm treated with PA5oct phage. Untreated biofilm was used as a control. Error bars denote SD. The results displayed are the mean of three independent experiments. Statistical analysis was made by the ANOVA test to compare data of treated biofilm versus control native biofilm at 40 min time point. The examples of interferograms (40 min) for PAO1 biofilm treated with active and inactivated PA5oct phages as well as control (from the top of a right-hand panel).
    Trypticase Soy Broth, supplied by Nissui Pharmaceutical, used in various techniques. Bioz Stars score: 92/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sanofi trypticase soy broth
    Real time measurement of biofilm permeability, after 4 h of phage treatment. ( A ) Membrane coverage analysis by crystal violet (CV) staining and ImageJ imaging software. Native membrane was used as a control. ( B ) Laser interferometry analysis of <t>trypticase-soy</t> broth (TSB) medium diffusion through PAO1 biofilm treated with PA5oct phage. Untreated biofilm was used as a control. Error bars denote SD. The results displayed are the mean of three independent experiments. Statistical analysis was made by the ANOVA test to compare data of treated biofilm versus control native biofilm at 40 min time point. The examples of interferograms (40 min) for PAO1 biofilm treated with active and inactivated PA5oct phages as well as control (from the top of a right-hand panel).
    Trypticase Soy Broth, supplied by Sanofi, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Liofilchem tryptic soy broth
    Real time measurement of biofilm permeability, after 4 h of phage treatment. ( A ) Membrane coverage analysis by crystal violet (CV) staining and ImageJ imaging software. Native membrane was used as a control. ( B ) Laser interferometry analysis of <t>trypticase-soy</t> broth (TSB) medium diffusion through PAO1 biofilm treated with PA5oct phage. Untreated biofilm was used as a control. Error bars denote SD. The results displayed are the mean of three independent experiments. Statistical analysis was made by the ANOVA test to compare data of treated biofilm versus control native biofilm at 40 min time point. The examples of interferograms (40 min) for PAO1 biofilm treated with active and inactivated PA5oct phages as well as control (from the top of a right-hand panel).
    Tryptic Soy Broth, supplied by Liofilchem, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Scientific tryptic soy broth
    Real time measurement of biofilm permeability, after 4 h of phage treatment. ( A ) Membrane coverage analysis by crystal violet (CV) staining and ImageJ imaging software. Native membrane was used as a control. ( B ) Laser interferometry analysis of <t>trypticase-soy</t> broth (TSB) medium diffusion through PAO1 biofilm treated with PA5oct phage. Untreated biofilm was used as a control. Error bars denote SD. The results displayed are the mean of three independent experiments. Statistical analysis was made by the ANOVA test to compare data of treated biofilm versus control native biofilm at 40 min time point. The examples of interferograms (40 min) for PAO1 biofilm treated with active and inactivated PA5oct phages as well as control (from the top of a right-hand panel).
    Tryptic Soy Broth, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    HiMedia Laboratories trypticase soy broth
    Real time measurement of biofilm permeability, after 4 h of phage treatment. ( A ) Membrane coverage analysis by crystal violet (CV) staining and ImageJ imaging software. Native membrane was used as a control. ( B ) Laser interferometry analysis of <t>trypticase-soy</t> broth (TSB) medium diffusion through PAO1 biofilm treated with PA5oct phage. Untreated biofilm was used as a control. Error bars denote SD. The results displayed are the mean of three independent experiments. Statistical analysis was made by the ANOVA test to compare data of treated biofilm versus control native biofilm at 40 min time point. The examples of interferograms (40 min) for PAO1 biofilm treated with active and inactivated PA5oct phages as well as control (from the top of a right-hand panel).
    Trypticase Soy Broth, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Difco trypticase soy t soy broth
    Real time measurement of biofilm permeability, after 4 h of phage treatment. ( A ) Membrane coverage analysis by crystal violet (CV) staining and ImageJ imaging software. Native membrane was used as a control. ( B ) Laser interferometry analysis of <t>trypticase-soy</t> broth (TSB) medium diffusion through PAO1 biofilm treated with PA5oct phage. Untreated biofilm was used as a control. Error bars denote SD. The results displayed are the mean of three independent experiments. Statistical analysis was made by the ANOVA test to compare data of treated biofilm versus control native biofilm at 40 min time point. The examples of interferograms (40 min) for PAO1 biofilm treated with active and inactivated PA5oct phages as well as control (from the top of a right-hand panel).
    Trypticase Soy T Soy Broth, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Scharlab trypticase soy broth tsb
    Real time measurement of biofilm permeability, after 4 h of phage treatment. ( A ) Membrane coverage analysis by crystal violet (CV) staining and ImageJ imaging software. Native membrane was used as a control. ( B ) Laser interferometry analysis of <t>trypticase-soy</t> broth (TSB) medium diffusion through PAO1 biofilm treated with PA5oct phage. Untreated biofilm was used as a control. Error bars denote SD. The results displayed are the mean of three independent experiments. Statistical analysis was made by the ANOVA test to compare data of treated biofilm versus control native biofilm at 40 min time point. The examples of interferograms (40 min) for PAO1 biofilm treated with active and inactivated PA5oct phages as well as control (from the top of a right-hand panel).
    Trypticase Soy Broth Tsb, supplied by Scharlab, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher tryptic soy broth tsb
    Schematic representation of the experimental design of this study: (A) Strains fermentation in <t>TSB</t> and <t>SSM,</t> (B) Commercial mix culture fermentation in SSM and SBB.
    Tryptic Soy Broth Tsb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson tryptic soy broth tsb
    Specific activity of Caa. Growth of complementation strain in <t>TSB</t> and minimal media with fructose. A) Specific activity of purified Strep2-tagged CA enzymes. Substrates used for this assay were bicarbonate (50 mM, dark bars) and CO 2 (saturated solution, grey bars). CA activity was measured by comparing the rapid change of pH in the presence of CA to the slower pH change in the absence of CA. The pH change was monitored using phenol red and the absorbance A 557nm was measured for 60 sec. Average values from three experiments were plotted with error bars representing the standard deviation. B) Cultures of R. <t>eutropha</t> wild type (H16 - ●), Re2428 (○), Re2428/pCaa (▼), and Re2428pCaaB (∆) in TSB media. C) Growth in minimal media with 2% fructose (w vol -1 ). Values represent average from three replicates with standard deviation as error bars.
    Tryptic Soy Broth Tsb, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 576 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The biofilm formation by S. aureus and S. epidermidis cultivated in Basal medium (BM), Luria-Bertani broth (LB), Müller-Hinton broth (MH), or Trypticase soy broth (TSB) on 35-mm polystyrol adhesive plates. 72 hours-old biofilms were stained by crystal violet.

    Journal: Scientific Reports

    Article Title: Targeting microbial biofilms using Ficin, a nonspecific plant protease

    doi: 10.1038/srep46068

    Figure Lengend Snippet: The biofilm formation by S. aureus and S. epidermidis cultivated in Basal medium (BM), Luria-Bertani broth (LB), Müller-Hinton broth (MH), or Trypticase soy broth (TSB) on 35-mm polystyrol adhesive plates. 72 hours-old biofilms were stained by crystal violet.

    Article Snippet: The Müller-Hinton broth (Fluka) or Trypticase soy broth (Sigma) did not provide stable biofilm formation by both Staphylococcus aureus and Staphylococcus epidermidis , as is has been determined in preliminary studies , thus the modified Basal medium (BM) (glucose 5 g, peptone 7 g, MgSO4 × 7H2 O 2.0 g and CaCl2 × 2H2 O 0.05 g in 1.0 liter tap water) was used for the biofilm formation assays .

    Techniques: Staining

    Comparison of S. aureus ISP479C and ISP479R growth kinetics in vitro. Log-phase organisms were dually inoculated (10 3 CFU of each strain) into 10 ml of BHI broth, and the cultures were incubated on a rotary shaker (for aeration) at 37°C. At selected time points, aliquots were quantitatively cultured on BHI agar with or without erythromycin (10 μg/ml; as described in Materials and Methods). Data points represent the mean values from two independent experiments performed in duplicate. In no case was the deviation from the mean greater than 0.2 log CFU/ml; error bars were intentionally excluded to improve clarity. These results mirrored those of identical dual-culture experiments performed with nutrient or Trypticase soy broth (see Materials and Methods). These results were not significantly different from those for either organism cultured alone (data not shown).

    Journal: Infection and Immunity

    Article Title: In Vitro Resistance to Thrombin-Induced Platelet Microbicidal Protein Is Associated with Enhanced Progression and Hematogenous Dissemination in Experimental Staphylococcus aureus Infective Endocarditis

    doi:

    Figure Lengend Snippet: Comparison of S. aureus ISP479C and ISP479R growth kinetics in vitro. Log-phase organisms were dually inoculated (10 3 CFU of each strain) into 10 ml of BHI broth, and the cultures were incubated on a rotary shaker (for aeration) at 37°C. At selected time points, aliquots were quantitatively cultured on BHI agar with or without erythromycin (10 μg/ml; as described in Materials and Methods). Data points represent the mean values from two independent experiments performed in duplicate. In no case was the deviation from the mean greater than 0.2 log CFU/ml; error bars were intentionally excluded to improve clarity. These results mirrored those of identical dual-culture experiments performed with nutrient or Trypticase soy broth (see Materials and Methods). These results were not significantly different from those for either organism cultured alone (data not shown).

    Article Snippet: For these studies, organisms were inoculated (∼103 CFU/ml) into brain heart infusion (BHI) broth, nutrient broth, or Trypticase soy broth (all media were from Difco Laboratories, Detroit, Mich.) and monitored for CFU/ml at selected times ranging from 1 to 24 h, with constant rotary shaking at 37°C.

    Techniques: In Vitro, Incubation, Cell Culture

    Comparison of antibiotic maximal efficacies ( E max ) expressed as the reduction in the number of CFU from that for the control (left) or as a percentage of the reduction in metabolic activity (resorufin fluorescence; middle) or biofilm mass (crystal violet [CV] absorbance; right) compared to that for an untreated biofilm for strain ATCC 25923 (open bar) or ATCC 33591 (closed bars) grown in Trypticase soy broth supplemented with 1% glucose and 2% NaCl (TGN) or in artificial sputum medium (ASM). MEM, meropenem; VAN, vancomycin; LZD, linezolid; AZM, azithromycin; RIF, rifampin; CIP, ciprofloxacin; TOB, tobramycin. Values are means ± SEM. Statistical analyses were by one-way analysis of variance (ANOVA) with Tukey’s posttest for multiple comparisons; values with different letters are significantly different from each other ( P  

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Activity of Antibiotics against Staphylococcus aureus in an In Vitro Model of Biofilms in the Context of Cystic Fibrosis: Influence of the Culture Medium

    doi: 10.1128/AAC.00602-19

    Figure Lengend Snippet: Comparison of antibiotic maximal efficacies ( E max ) expressed as the reduction in the number of CFU from that for the control (left) or as a percentage of the reduction in metabolic activity (resorufin fluorescence; middle) or biofilm mass (crystal violet [CV] absorbance; right) compared to that for an untreated biofilm for strain ATCC 25923 (open bar) or ATCC 33591 (closed bars) grown in Trypticase soy broth supplemented with 1% glucose and 2% NaCl (TGN) or in artificial sputum medium (ASM). MEM, meropenem; VAN, vancomycin; LZD, linezolid; AZM, azithromycin; RIF, rifampin; CIP, ciprofloxacin; TOB, tobramycin. Values are means ± SEM. Statistical analyses were by one-way analysis of variance (ANOVA) with Tukey’s posttest for multiple comparisons; values with different letters are significantly different from each other ( P  

    Article Snippet: Two media were used in parallel, namely, Trypticase soy broth (VWR, Radnor, PA) supplemented with 1% glucose and 2% NaCl (TGN) ( , ) or artificial sputum medium (ASM) (adapted from previous work [ , , ]).

    Techniques: Activity Assay, Fluorescence

    Metabolic activity in planktonic cultures or biofilm cultures grown in Trypticase soy broth supplemented with 1% glucose and 2% NaCl (TGN) or in artificial sputum medium (ASM). (Left) Resorufin fluorescence signal recorded after 30 min of incubation of planktonic bacteria at increasing inocula with 10-mg/liter resazurin. (Right) Resorufin fluorescence signal recorded after 30 min of incubation of a 24-h-old biofilm with 10-mg/liter resazurin. Data are means ± SD for triplicates in a single experiment or means ± SEM from at least 3 independent experiments performed in triplicate. Statistical analyses comparing strains in each individual medium were performed by one-way analysis of variance (ANOVA) with Tukey’s posttest for multiple comparisons; values with different letters are significantly different from each other ( P  

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Activity of Antibiotics against Staphylococcus aureus in an In Vitro Model of Biofilms in the Context of Cystic Fibrosis: Influence of the Culture Medium

    doi: 10.1128/AAC.00602-19

    Figure Lengend Snippet: Metabolic activity in planktonic cultures or biofilm cultures grown in Trypticase soy broth supplemented with 1% glucose and 2% NaCl (TGN) or in artificial sputum medium (ASM). (Left) Resorufin fluorescence signal recorded after 30 min of incubation of planktonic bacteria at increasing inocula with 10-mg/liter resazurin. (Right) Resorufin fluorescence signal recorded after 30 min of incubation of a 24-h-old biofilm with 10-mg/liter resazurin. Data are means ± SD for triplicates in a single experiment or means ± SEM from at least 3 independent experiments performed in triplicate. Statistical analyses comparing strains in each individual medium were performed by one-way analysis of variance (ANOVA) with Tukey’s posttest for multiple comparisons; values with different letters are significantly different from each other ( P  

    Article Snippet: Two media were used in parallel, namely, Trypticase soy broth (VWR, Radnor, PA) supplemented with 1% glucose and 2% NaCl (TGN) ( , ) or artificial sputum medium (ASM) (adapted from previous work [ , , ]).

    Techniques: Activity Assay, Fluorescence, Incubation

    Microbicidal kinetics. P. aeruginosa strain 2 was exposed to the indicated concentrations of novispirin G10 in a medium composed of 100 mM NaCl, 10 mM sodium phosphate buffer (pH 7.4), and 0.3 mg of Trypticase soy broth powder/ml. Aliquots were removed after 5 and 10 min, diluted, spread on Trypticase soy agar plates, and incubated overnight to allow colony development.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns

    doi: 10.1128/AAC.46.6.1837-1844.2002

    Figure Lengend Snippet: Microbicidal kinetics. P. aeruginosa strain 2 was exposed to the indicated concentrations of novispirin G10 in a medium composed of 100 mM NaCl, 10 mM sodium phosphate buffer (pH 7.4), and 0.3 mg of Trypticase soy broth powder/ml. Aliquots were removed after 5 and 10 min, diluted, spread on Trypticase soy agar plates, and incubated overnight to allow colony development.

    Article Snippet: The overlay agar consisted of 6% (wt/vol) Trypticase soy broth and 1% agarose in phosphate-buffered saline for all assays.

    Techniques: Incubation

    The bioactive molecules are secreted. Strains of C. citroniae and C. aldenense that showed strong activity against hilA expression were cultured under anaerobic conditions in Trypticase soy broth supplemented with menadione and hemin for at least 2 days, and the cells were separated from the supernatants through centrifugation. Both the cells and the supernatants were then extracted with ethyl acetate, and dried extracts were added to LB broth, which was used to culture the Salmonella hilA :: gfp reporter strain for measurements of hilA expression. Results shown are the averages of 3 individual measurements, and bars show the standard errors of the means. ***, P

    Journal: mBio

    Article Title: Antivirulence Activity of the Human Gut Metabolome

    doi: 10.1128/mBio.01183-14

    Figure Lengend Snippet: The bioactive molecules are secreted. Strains of C. citroniae and C. aldenense that showed strong activity against hilA expression were cultured under anaerobic conditions in Trypticase soy broth supplemented with menadione and hemin for at least 2 days, and the cells were separated from the supernatants through centrifugation. Both the cells and the supernatants were then extracted with ethyl acetate, and dried extracts were added to LB broth, which was used to culture the Salmonella hilA :: gfp reporter strain for measurements of hilA expression. Results shown are the averages of 3 individual measurements, and bars show the standard errors of the means. ***, P

    Article Snippet: Where indicated, intestinal commensals were cultured under anaerobic conditions in Trypticase soy broth (Oxoid, Cambridge, England) supplemented with menadione (1 µg/ml; Sigma-Aldrich) and hemin (5 µg/ml; BDH, Radnor, PA) for at least 2 days.

    Techniques: Activity Assay, Expressing, Cell Culture, Centrifugation

    A human isolate of Clostridium citroniae produces strong activity against Salmonella invasion gene expression. Microbial isolates from a chemostat culture showing activity against hilA were tested individually for biological activity. Isolates were cultured under anaerobic conditions in Trypticase soy broth supplemented with menadione and hemin for at least 2 days, and the cultures were extracted with ethyl acetate. Dried extracts were added to LB broth, which was used to culture a Salmonella hilA :: gfp reporter strain. GFP production was then monitored through flow cytometry. Results shown are the averages of three individual measurements ( n = 3), except for the control culture, where six cultures were used ( n = 6). Bars indicate the standard errors of the means.

    Journal: mBio

    Article Title: Antivirulence Activity of the Human Gut Metabolome

    doi: 10.1128/mBio.01183-14

    Figure Lengend Snippet: A human isolate of Clostridium citroniae produces strong activity against Salmonella invasion gene expression. Microbial isolates from a chemostat culture showing activity against hilA were tested individually for biological activity. Isolates were cultured under anaerobic conditions in Trypticase soy broth supplemented with menadione and hemin for at least 2 days, and the cultures were extracted with ethyl acetate. Dried extracts were added to LB broth, which was used to culture a Salmonella hilA :: gfp reporter strain. GFP production was then monitored through flow cytometry. Results shown are the averages of three individual measurements ( n = 3), except for the control culture, where six cultures were used ( n = 6). Bars indicate the standard errors of the means.

    Article Snippet: Where indicated, intestinal commensals were cultured under anaerobic conditions in Trypticase soy broth (Oxoid, Cambridge, England) supplemented with menadione (1 µg/ml; Sigma-Aldrich) and hemin (5 µg/ml; BDH, Radnor, PA) for at least 2 days.

    Techniques: Activity Assay, Expressing, Cell Culture, Flow Cytometry, Cytometry

    Closely related Clostridium species are involved in the production of the inhibitory molecules. (A) Strains from diverse Clostridium species were tested for biological activity against hilA . Strains were cultured under anaerobic conditions in Trypticase soy broth supplemented with menadione and hemin for at least 2 days, and the cultures were extracted with ethyl acetate. Dried extracts were added to LB broth, which was used to culture a Salmonella hilA :: gfp reporter strain. GFP production was then monitored through flow cytometry. Results shown are the averages of three individual measurements ( n = 3), except for the control culture, where six cultures were used ( n = 6). Bars indicate the standard errors of the means. (B) Production of the inhibitory molecule by select Clostridium strains was confirmed through RT-PCR targeting hilA . Results shown are the averages of 2 to 6 individual measurements, and bars show the standard errors of the means. *, P

    Journal: mBio

    Article Title: Antivirulence Activity of the Human Gut Metabolome

    doi: 10.1128/mBio.01183-14

    Figure Lengend Snippet: Closely related Clostridium species are involved in the production of the inhibitory molecules. (A) Strains from diverse Clostridium species were tested for biological activity against hilA . Strains were cultured under anaerobic conditions in Trypticase soy broth supplemented with menadione and hemin for at least 2 days, and the cultures were extracted with ethyl acetate. Dried extracts were added to LB broth, which was used to culture a Salmonella hilA :: gfp reporter strain. GFP production was then monitored through flow cytometry. Results shown are the averages of three individual measurements ( n = 3), except for the control culture, where six cultures were used ( n = 6). Bars indicate the standard errors of the means. (B) Production of the inhibitory molecule by select Clostridium strains was confirmed through RT-PCR targeting hilA . Results shown are the averages of 2 to 6 individual measurements, and bars show the standard errors of the means. *, P

    Article Snippet: Where indicated, intestinal commensals were cultured under anaerobic conditions in Trypticase soy broth (Oxoid, Cambridge, England) supplemented with menadione (1 µg/ml; Sigma-Aldrich) and hemin (5 µg/ml; BDH, Radnor, PA) for at least 2 days.

    Techniques: Activity Assay, Cell Culture, Flow Cytometry, Cytometry, Reverse Transcription Polymerase Chain Reaction

    Real time measurement of biofilm permeability, after 4 h of phage treatment. ( A ) Membrane coverage analysis by crystal violet (CV) staining and ImageJ imaging software. Native membrane was used as a control. ( B ) Laser interferometry analysis of trypticase-soy broth (TSB) medium diffusion through PAO1 biofilm treated with PA5oct phage. Untreated biofilm was used as a control. Error bars denote SD. The results displayed are the mean of three independent experiments. Statistical analysis was made by the ANOVA test to compare data of treated biofilm versus control native biofilm at 40 min time point. The examples of interferograms (40 min) for PAO1 biofilm treated with active and inactivated PA5oct phages as well as control (from the top of a right-hand panel).

    Journal: Viruses

    Article Title: Pseudomonas aeruginosa PA5oct Jumbo Phage Impacts Planktonic and Biofilm Population and Reduces Its Host Virulence

    doi: 10.3390/v11121089

    Figure Lengend Snippet: Real time measurement of biofilm permeability, after 4 h of phage treatment. ( A ) Membrane coverage analysis by crystal violet (CV) staining and ImageJ imaging software. Native membrane was used as a control. ( B ) Laser interferometry analysis of trypticase-soy broth (TSB) medium diffusion through PAO1 biofilm treated with PA5oct phage. Untreated biofilm was used as a control. Error bars denote SD. The results displayed are the mean of three independent experiments. Statistical analysis was made by the ANOVA test to compare data of treated biofilm versus control native biofilm at 40 min time point. The examples of interferograms (40 min) for PAO1 biofilm treated with active and inactivated PA5oct phages as well as control (from the top of a right-hand panel).

    Article Snippet: PAO1 biofilm was formed on the surface of Nephrophane membrane during the incubation for 72 h at 37 °C in Trypticase-Soy broth (TSB, bioMerieux, Craponne, France).

    Techniques: Permeability, Staining, Imaging, Software, Diffusion-based Assay

    Schematic representation of the experimental design of this study: (A) Strains fermentation in TSB and SSM, (B) Commercial mix culture fermentation in SSM and SBB.

    Journal: Foods

    Article Title: Arthrospira platensis as Natural Fermentation Booster for Milk and Soy Fermented Beverages

    doi: 10.3390/foods9030350

    Figure Lengend Snippet: Schematic representation of the experimental design of this study: (A) Strains fermentation in TSB and SSM, (B) Commercial mix culture fermentation in SSM and SBB.

    Article Snippet: Three different growth substrates were considered: Tryptic Soy Broth (TSB) (Oxoid), reconstituted skim milk powder (SSM) (Oxoid, Ltd., Basingstoke, United Kingdom), and a commercial soy-based beverage (SBB).

    Techniques:

    Specific activity of Caa. Growth of complementation strain in TSB and minimal media with fructose. A) Specific activity of purified Strep2-tagged CA enzymes. Substrates used for this assay were bicarbonate (50 mM, dark bars) and CO 2 (saturated solution, grey bars). CA activity was measured by comparing the rapid change of pH in the presence of CA to the slower pH change in the absence of CA. The pH change was monitored using phenol red and the absorbance A 557nm was measured for 60 sec. Average values from three experiments were plotted with error bars representing the standard deviation. B) Cultures of R. eutropha wild type (H16 - ●), Re2428 (○), Re2428/pCaa (▼), and Re2428pCaaB (∆) in TSB media. C) Growth in minimal media with 2% fructose (w vol -1 ). Values represent average from three replicates with standard deviation as error bars.

    Journal: AMB Express

    Article Title: Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16

    doi: 10.1186/2191-0855-4-2

    Figure Lengend Snippet: Specific activity of Caa. Growth of complementation strain in TSB and minimal media with fructose. A) Specific activity of purified Strep2-tagged CA enzymes. Substrates used for this assay were bicarbonate (50 mM, dark bars) and CO 2 (saturated solution, grey bars). CA activity was measured by comparing the rapid change of pH in the presence of CA to the slower pH change in the absence of CA. The pH change was monitored using phenol red and the absorbance A 557nm was measured for 60 sec. Average values from three experiments were plotted with error bars representing the standard deviation. B) Cultures of R. eutropha wild type (H16 - ●), Re2428 (○), Re2428/pCaa (▼), and Re2428pCaaB (∆) in TSB media. C) Growth in minimal media with 2% fructose (w vol -1 ). Values represent average from three replicates with standard deviation as error bars.

    Article Snippet: Growth media and cultivation conditions R. eutropha strains were propagated in tryptic soy broth (TSB) (Becton Dickinson, Sparks, MD) or minimal medium [ ] with fructose at a final concentration of 1% or 2% (w vol-1 ), or pyruvate, lactate, succinate, or formate, each at a final concentration of 0.2% (w vol-1 ) or (vol vol-1 ).

    Techniques: Activity Assay, Cellular Antioxidant Activity Assay, Purification, Size-exclusion Chromatography, Standard Deviation

    Growth of the quadruple deletion mutant on TSB + 10% CO 2 . Cultures of R. eutropha H16 (□), R. eutropha CA quadruple deletion mutant Re2436 (H16 ∆ can ∆ can2 ∆ caa ∆ cag - ●) and the complemented strains, Re2436/pCan (○), Re2436/pCan2 (▼), Re2436/Caa (∆) and Re2436/pCag (■) in TSB media in the presence of 10% CO 2 . Values represent average from three replicates with standard deviation as error bars.

    Journal: AMB Express

    Article Title: Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16

    doi: 10.1186/2191-0855-4-2

    Figure Lengend Snippet: Growth of the quadruple deletion mutant on TSB + 10% CO 2 . Cultures of R. eutropha H16 (□), R. eutropha CA quadruple deletion mutant Re2436 (H16 ∆ can ∆ can2 ∆ caa ∆ cag - ●) and the complemented strains, Re2436/pCan (○), Re2436/pCan2 (▼), Re2436/Caa (∆) and Re2436/pCag (■) in TSB media in the presence of 10% CO 2 . Values represent average from three replicates with standard deviation as error bars.

    Article Snippet: Growth media and cultivation conditions R. eutropha strains were propagated in tryptic soy broth (TSB) (Becton Dickinson, Sparks, MD) or minimal medium [ ] with fructose at a final concentration of 1% or 2% (w vol-1 ), or pyruvate, lactate, succinate, or formate, each at a final concentration of 0.2% (w vol-1 ) or (vol vol-1 ).

    Techniques: Mutagenesis, Cellular Antioxidant Activity Assay, Standard Deviation