tryptic soy broth tsb Search Results


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  • 99
    Thermo Fisher tryptic soy broth tsb
    ESR determination of hydroxyl radicals formed in cultures of S. aureus treated with hydrogen peroxide or antibiotics. Hydroxyl-radical signals were monitored in <t>BHI</t> (A) or <t>TSB</t> (B) medium (filled diamonds) or in cultures of S. aureus grown in the corresponding
    Tryptic Soy Broth Tsb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore tryptic soy broth tsb
    Adherence of S. aureus Newman complemented mutants grown in <t>TSB</t> and iron restricted conditions to desquamated nasal epithelial cells . The ability of mutants of strain Newman carrying complementing pCU1 plasmids carrying surface protein genes to adhere to desquamated nasal epithelial cells was tested. Strains Newman clfA , Newman clfA isdA clfB sdrCDE , Newman clfA isdA clfB sdrCDE (pCU1), Newman clfA isdA clfB sdrCDE (pCU1 clfB + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrC + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrD + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrE + ),) Newman clfA isdA clfB sdrCDE (pCU1 isdAB + ) and Newman clfA isdA clfB sdrCDE (pCU1 isdB + ) grown to the exponential phase in (A) TSB and to the stationary phase in (B) <t>RPMI</t> were tested for adherence. Counts represent the number of bacterial cells adhering to 100 squamous cells. Results are expressed as the mean of triplicate experiments +/- standard deviations.
    Tryptic Soy Broth Tsb, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 354 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Difco tryptic soy broth tsb
    Fisetin at the concentrations of 32 μg/ml does not affect the growth characteristics of <t>SC19.</t> (A) SC19 was co-incubated with 0, 8, 16, 32, 64, 128 μg/ml fisetin in <t>TSB</t> containing 10% of newborn bovine serum, and the growth characteristic of SC19 was measured by OD 600 every hour. (B) The effect of fisetin (0, 8, 16, 32 μg/ml) on the expression of suilysin in SC19 supernatant was investigated at 0, 4, 8, and 16 h by western blotting. Exogenous addition of BSA was detected as an internal control using an anti-BSA antibody. The medium supernatant without addition of SC19 was detected by western blot assay at 0 h.
    Tryptic Soy Broth Tsb, supplied by Difco, used in various techniques. Bioz Stars score: 99/100, based on 692 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Valiant tryptic soy broth tsb
    Fisetin at the concentrations of 32 μg/ml does not affect the growth characteristics of <t>SC19.</t> (A) SC19 was co-incubated with 0, 8, 16, 32, 64, 128 μg/ml fisetin in <t>TSB</t> containing 10% of newborn bovine serum, and the growth characteristic of SC19 was measured by OD 600 every hour. (B) The effect of fisetin (0, 8, 16, 32 μg/ml) on the expression of suilysin in SC19 supernatant was investigated at 0, 4, 8, and 16 h by western blotting. Exogenous addition of BSA was detected as an internal control using an anti-BSA antibody. The medium supernatant without addition of SC19 was detected by western blot assay at 0 h.
    Tryptic Soy Broth Tsb, supplied by Valiant, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Becton Dickinson tryptic soy broth tsb
    Specific activity of Caa. Growth of complementation strain in <t>TSB</t> and minimal media with fructose. A) Specific activity of purified Strep2-tagged CA enzymes. Substrates used for this assay were bicarbonate (50 mM, dark bars) and CO 2 (saturated solution, grey bars). CA activity was measured by comparing the rapid change of pH in the presence of CA to the slower pH change in the absence of CA. The pH change was monitored using phenol red and the absorbance A 557nm was measured for 60 sec. Average values from three experiments were plotted with error bars representing the standard deviation. B) Cultures of R. <t>eutropha</t> wild type (H16 - ●), Re2428 (○), Re2428/pCaa (▼), and Re2428pCaaB (∆) in TSB media. C) Growth in minimal media with 2% fructose (w vol -1 ). Values represent average from three replicates with standard deviation as error bars.
    Tryptic Soy Broth Tsb, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 97/100, based on 581 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Merck KGaA tryptic soy broth tsb
    Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas <t>brassicacearum</t> MA250 pure culture in <t>TSB,</t> 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each
    Tryptic Soy Broth Tsb, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 98/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    HiMedia Laboratories tryptic soy broth tsb
    Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas <t>brassicacearum</t> MA250 pure culture in <t>TSB,</t> 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each
    Tryptic Soy Broth Tsb, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Alpha Biosciences tryptic soy broth tsb
    Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas <t>brassicacearum</t> MA250 pure culture in <t>TSB,</t> 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each
    Tryptic Soy Broth Tsb, supplied by Alpha Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Merck & Co tryptic soy broth tsb
    S. epidermidis bacteria obtained from high dormancy biofilms induce a lower activation of murine BMDCs. Cell suspensions obtained from 48 h biofilms grown in <t>TSB</t> 1 %G (dormant biofilms), 48 h biofilms grown in TSB 1 %G+Mg 2+ (non-dormant biofilms) or original planktonic cultures (planktonic) were used to stimulate BMDCs in vitro . The concentration of the proinflammatory cytokines (a) TNF-α, (b) IL-12 and (c) IL-6, and of anti-inflammatory cytokine (d) IL-10, was evaluated by ELISA in the culture supernatants. Results shown for each cytokine are representative of two independent experiments that generated concordant results. Statistical analysis was carried out by one-way ANOVA with Tukey's multiple comparison test.
    Tryptic Soy Broth Tsb, supplied by Merck & Co, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Avantor tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb, supplied by Avantor, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Cardinal Health tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb, supplied by Cardinal Health, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    scharlau tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb, supplied by scharlau, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Guangdong Huankai Microbial tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb, supplied by Guangdong Huankai Microbial, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Lab M Ltd tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb, supplied by Lab M Ltd, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Liofilchem tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb, supplied by Liofilchem, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Cellgro tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb, supplied by Cellgro, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Fisher Scientific tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    bioMerieux tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb, supplied by bioMerieux, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Applichem tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb, supplied by Applichem, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Carolina Biological tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb, supplied by Carolina Biological, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Hardy Diagnostics tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb, supplied by Hardy Diagnostics, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Teknova tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb, supplied by Teknova, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Carl Roth GmbH tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Scharlab trypticase soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Trypticase Soy Broth Tsb, supplied by Scharlab, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Becton Dickinson tryptic soy broth tsb tubes
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Tryptic Soy Broth Tsb Tubes, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Becton Dickinson bacto tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Bacto Tryptic Soy Broth Tsb, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLife Solutions sterile tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Sterile Tryptic Soy Broth Tsb, supplied by BioLife Solutions, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson sterile tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Sterile Tryptic Soy Broth Tsb, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson bactotm tryptic soy broth tsb
    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus <t>HG003</t> in <t>TSB</t> supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.
    Bactotm Tryptic Soy Broth Tsb, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco tryptic soy broth tsb 10
    Soluble thiols analysis. HPLC analysis of nonprotein thiols in S. maltophilia Sm777 grown in <t>TSB/10</t> without (A) or supplemented (B) with 500 µM of CdCl 2 . The arrow indicates cysteine peack. N -acetyl-L-cysteine (NAC) was used as an internal standard.
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    Image Search Results


    ESR determination of hydroxyl radicals formed in cultures of S. aureus treated with hydrogen peroxide or antibiotics. Hydroxyl-radical signals were monitored in BHI (A) or TSB (B) medium (filled diamonds) or in cultures of S. aureus grown in the corresponding

    Journal: Applied and Environmental Microbiology

    Article Title: Catalase Expression Is Modulated by Vancomycin and Ciprofloxacin and Influences the Formation of Free Radicals in Staphylococcus aureus Cultures

    doi: 10.1128/AEM.01199-15

    Figure Lengend Snippet: ESR determination of hydroxyl radicals formed in cultures of S. aureus treated with hydrogen peroxide or antibiotics. Hydroxyl-radical signals were monitored in BHI (A) or TSB (B) medium (filled diamonds) or in cultures of S. aureus grown in the corresponding

    Article Snippet: Staphylococcus aureus Newman (NTCT 8178) was cultivated in 25 ml of tryptic soy broth (TSB) or brain heart infusion broth (BHI) (Oxoid, Denmark) in a 250-ml narrow-neck Erlenmeyer flask at 37°C and 200 rpm.

    Techniques: Electron Paramagnetic Resonance

    Adherence of S. aureus Newman complemented mutants grown in TSB and iron restricted conditions to desquamated nasal epithelial cells . The ability of mutants of strain Newman carrying complementing pCU1 plasmids carrying surface protein genes to adhere to desquamated nasal epithelial cells was tested. Strains Newman clfA , Newman clfA isdA clfB sdrCDE , Newman clfA isdA clfB sdrCDE (pCU1), Newman clfA isdA clfB sdrCDE (pCU1 clfB + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrC + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrD + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrE + ),) Newman clfA isdA clfB sdrCDE (pCU1 isdAB + ) and Newman clfA isdA clfB sdrCDE (pCU1 isdB + ) grown to the exponential phase in (A) TSB and to the stationary phase in (B) RPMI were tested for adherence. Counts represent the number of bacterial cells adhering to 100 squamous cells. Results are expressed as the mean of triplicate experiments +/- standard deviations.

    Journal: BMC Microbiology

    Article Title: Surface proteins that promote adherence of Staphylococcus aureus to human desquamated nasal epithelial cells

    doi: 10.1186/1471-2180-9-22

    Figure Lengend Snippet: Adherence of S. aureus Newman complemented mutants grown in TSB and iron restricted conditions to desquamated nasal epithelial cells . The ability of mutants of strain Newman carrying complementing pCU1 plasmids carrying surface protein genes to adhere to desquamated nasal epithelial cells was tested. Strains Newman clfA , Newman clfA isdA clfB sdrCDE , Newman clfA isdA clfB sdrCDE (pCU1), Newman clfA isdA clfB sdrCDE (pCU1 clfB + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrC + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrD + ), Newman clfA isdA clfB sdrCDE (pCU1 sdrE + ),) Newman clfA isdA clfB sdrCDE (pCU1 isdAB + ) and Newman clfA isdA clfB sdrCDE (pCU1 isdB + ) grown to the exponential phase in (A) TSB and to the stationary phase in (B) RPMI were tested for adherence. Counts represent the number of bacterial cells adhering to 100 squamous cells. Results are expressed as the mean of triplicate experiments +/- standard deviations.

    Article Snippet: S. aureus strains were grown on tryptic soy agar (TSA; Oxoid), tryptic soy broth (TSB) or RPMI 1640 (Sigma).

    Techniques:

    Adherence of Newman mutants to desquamated nasal epithelial cells . The ability of (A) Newman clfA , Newman clfA clfB , Newman clfA sdrCDE and Newman clfA clfB sdrCDE grown to exponential phase in TSB and (B) Newman, Newman clfA , Newman clfA clfB , Newman clfA sdrCDE , Newman clfA isdA , Newman clfA isdA sdrCDE , Newman clfA clfB sdrCDE , Newman clfA isdA clfB , and Newman clfA isdA clfB sdrCDE grown to stationary phase in RPMI to adhere to desquamated human nasal epithelial cells was measured. The tenth track is a control without S. aureus showing background due to adherent bacteria from the donor. Counts represent the number of bacterial cells adhering to 100 squamous cells. Results are expressed as the mean of triplicate experiments +/- standard deviations.

    Journal: BMC Microbiology

    Article Title: Surface proteins that promote adherence of Staphylococcus aureus to human desquamated nasal epithelial cells

    doi: 10.1186/1471-2180-9-22

    Figure Lengend Snippet: Adherence of Newman mutants to desquamated nasal epithelial cells . The ability of (A) Newman clfA , Newman clfA clfB , Newman clfA sdrCDE and Newman clfA clfB sdrCDE grown to exponential phase in TSB and (B) Newman, Newman clfA , Newman clfA clfB , Newman clfA sdrCDE , Newman clfA isdA , Newman clfA isdA sdrCDE , Newman clfA clfB sdrCDE , Newman clfA isdA clfB , and Newman clfA isdA clfB sdrCDE grown to stationary phase in RPMI to adhere to desquamated human nasal epithelial cells was measured. The tenth track is a control without S. aureus showing background due to adherent bacteria from the donor. Counts represent the number of bacterial cells adhering to 100 squamous cells. Results are expressed as the mean of triplicate experiments +/- standard deviations.

    Article Snippet: S. aureus strains were grown on tryptic soy agar (TSA; Oxoid), tryptic soy broth (TSB) or RPMI 1640 (Sigma).

    Techniques:

    Fisetin at the concentrations of 32 μg/ml does not affect the growth characteristics of SC19. (A) SC19 was co-incubated with 0, 8, 16, 32, 64, 128 μg/ml fisetin in TSB containing 10% of newborn bovine serum, and the growth characteristic of SC19 was measured by OD 600 every hour. (B) The effect of fisetin (0, 8, 16, 32 μg/ml) on the expression of suilysin in SC19 supernatant was investigated at 0, 4, 8, and 16 h by western blotting. Exogenous addition of BSA was detected as an internal control using an anti-BSA antibody. The medium supernatant without addition of SC19 was detected by western blot assay at 0 h.

    Journal: Frontiers in Microbiology

    Article Title: Fisetin Lowers Streptococcus suis serotype 2 Pathogenicity in Mice by Inhibiting the Hemolytic Activity of Suilysin

    doi: 10.3389/fmicb.2018.01723

    Figure Lengend Snippet: Fisetin at the concentrations of 32 μg/ml does not affect the growth characteristics of SC19. (A) SC19 was co-incubated with 0, 8, 16, 32, 64, 128 μg/ml fisetin in TSB containing 10% of newborn bovine serum, and the growth characteristic of SC19 was measured by OD 600 every hour. (B) The effect of fisetin (0, 8, 16, 32 μg/ml) on the expression of suilysin in SC19 supernatant was investigated at 0, 4, 8, and 16 h by western blotting. Exogenous addition of BSA was detected as an internal control using an anti-BSA antibody. The medium supernatant without addition of SC19 was detected by western blot assay at 0 h.

    Article Snippet: SC19 and Δsly were grown in tryptic soy broth (TSB) or plated on tryptic soy agar (TSA) (Difco Laboratories, Detroit, MI, United States) with 10% newborn bovine serum added (Sijiqing Biological Engineering Materials Co., Ltd., Hangzhou, China) at 37°C.

    Techniques: Incubation, Expressing, Western Blot

    Inhibition of suilysin-induced hemolysis by fisetin: (A) The culture supernatant of SC19 cultures causes sheep erythrocyte hemolysis. Hemolysis was measured by hemoglobin release at OD 543 . (B) Chemical structure of fisetin. (C) After SC19 cultures were incubated with fisetin (0, 2, 4, 8, 16, and 32 μg/ml) at 37°C for 18 h, 125 μl culture supernatant was incubated with 875 μl of PBS containing a 2% defibrinated sheep blood at 37°C for 30 min. Fisetin significantly inhibited the hemolytic activity of the supernatant of SC19 cultured in TSB with 10% newborn bovine serum with 32 μg/ml fisetin. (D) After SC19 was cultured at 37°C for 18 h, 125 μl culture supernatant incubated with fisetin (0, 2, 4, 8, 16, and 32 μg/ml) at 37°C for 30 min was incubated with 875 μl of PBS containing a 2% defibrinated sheep blood at 37°C for 30 min. The inhibitor effect of fisetin on the suilysin in the supernatant of SC19 is dose dependent. Statistical analyses were performed using the two-tailed unpaired t -test. Statistically significant differences are indicated. ∗ P

    Journal: Frontiers in Microbiology

    Article Title: Fisetin Lowers Streptococcus suis serotype 2 Pathogenicity in Mice by Inhibiting the Hemolytic Activity of Suilysin

    doi: 10.3389/fmicb.2018.01723

    Figure Lengend Snippet: Inhibition of suilysin-induced hemolysis by fisetin: (A) The culture supernatant of SC19 cultures causes sheep erythrocyte hemolysis. Hemolysis was measured by hemoglobin release at OD 543 . (B) Chemical structure of fisetin. (C) After SC19 cultures were incubated with fisetin (0, 2, 4, 8, 16, and 32 μg/ml) at 37°C for 18 h, 125 μl culture supernatant was incubated with 875 μl of PBS containing a 2% defibrinated sheep blood at 37°C for 30 min. Fisetin significantly inhibited the hemolytic activity of the supernatant of SC19 cultured in TSB with 10% newborn bovine serum with 32 μg/ml fisetin. (D) After SC19 was cultured at 37°C for 18 h, 125 μl culture supernatant incubated with fisetin (0, 2, 4, 8, 16, and 32 μg/ml) at 37°C for 30 min was incubated with 875 μl of PBS containing a 2% defibrinated sheep blood at 37°C for 30 min. The inhibitor effect of fisetin on the suilysin in the supernatant of SC19 is dose dependent. Statistical analyses were performed using the two-tailed unpaired t -test. Statistically significant differences are indicated. ∗ P

    Article Snippet: SC19 and Δsly were grown in tryptic soy broth (TSB) or plated on tryptic soy agar (TSA) (Difco Laboratories, Detroit, MI, United States) with 10% newborn bovine serum added (Sijiqing Biological Engineering Materials Co., Ltd., Hangzhou, China) at 37°C.

    Techniques: Inhibition, Incubation, Activity Assay, Cell Culture, Two Tailed Test

    Specific activity of Caa. Growth of complementation strain in TSB and minimal media with fructose. A) Specific activity of purified Strep2-tagged CA enzymes. Substrates used for this assay were bicarbonate (50 mM, dark bars) and CO 2 (saturated solution, grey bars). CA activity was measured by comparing the rapid change of pH in the presence of CA to the slower pH change in the absence of CA. The pH change was monitored using phenol red and the absorbance A 557nm was measured for 60 sec. Average values from three experiments were plotted with error bars representing the standard deviation. B) Cultures of R. eutropha wild type (H16 - ●), Re2428 (○), Re2428/pCaa (▼), and Re2428pCaaB (∆) in TSB media. C) Growth in minimal media with 2% fructose (w vol -1 ). Values represent average from three replicates with standard deviation as error bars.

    Journal: AMB Express

    Article Title: Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16

    doi: 10.1186/2191-0855-4-2

    Figure Lengend Snippet: Specific activity of Caa. Growth of complementation strain in TSB and minimal media with fructose. A) Specific activity of purified Strep2-tagged CA enzymes. Substrates used for this assay were bicarbonate (50 mM, dark bars) and CO 2 (saturated solution, grey bars). CA activity was measured by comparing the rapid change of pH in the presence of CA to the slower pH change in the absence of CA. The pH change was monitored using phenol red and the absorbance A 557nm was measured for 60 sec. Average values from three experiments were plotted with error bars representing the standard deviation. B) Cultures of R. eutropha wild type (H16 - ●), Re2428 (○), Re2428/pCaa (▼), and Re2428pCaaB (∆) in TSB media. C) Growth in minimal media with 2% fructose (w vol -1 ). Values represent average from three replicates with standard deviation as error bars.

    Article Snippet: Growth media and cultivation conditions R. eutropha strains were propagated in tryptic soy broth (TSB) (Becton Dickinson, Sparks, MD) or minimal medium [ ] with fructose at a final concentration of 1% or 2% (w vol-1 ), or pyruvate, lactate, succinate, or formate, each at a final concentration of 0.2% (w vol-1 ) or (vol vol-1 ).

    Techniques: Activity Assay, Cellular Antioxidant Activity Assay, Purification, Size-exclusion Chromatography, Standard Deviation

    Growth of the quadruple deletion mutant on TSB + 10% CO 2 . Cultures of R. eutropha H16 (□), R. eutropha CA quadruple deletion mutant Re2436 (H16 ∆ can ∆ can2 ∆ caa ∆ cag - ●) and the complemented strains, Re2436/pCan (○), Re2436/pCan2 (▼), Re2436/Caa (∆) and Re2436/pCag (■) in TSB media in the presence of 10% CO 2 . Values represent average from three replicates with standard deviation as error bars.

    Journal: AMB Express

    Article Title: Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16

    doi: 10.1186/2191-0855-4-2

    Figure Lengend Snippet: Growth of the quadruple deletion mutant on TSB + 10% CO 2 . Cultures of R. eutropha H16 (□), R. eutropha CA quadruple deletion mutant Re2436 (H16 ∆ can ∆ can2 ∆ caa ∆ cag - ●) and the complemented strains, Re2436/pCan (○), Re2436/pCan2 (▼), Re2436/Caa (∆) and Re2436/pCag (■) in TSB media in the presence of 10% CO 2 . Values represent average from three replicates with standard deviation as error bars.

    Article Snippet: Growth media and cultivation conditions R. eutropha strains were propagated in tryptic soy broth (TSB) (Becton Dickinson, Sparks, MD) or minimal medium [ ] with fructose at a final concentration of 1% or 2% (w vol-1 ), or pyruvate, lactate, succinate, or formate, each at a final concentration of 0.2% (w vol-1 ) or (vol vol-1 ).

    Techniques: Mutagenesis, Cellular Antioxidant Activity Assay, Standard Deviation

    Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas brassicacearum MA250 pure culture in TSB, 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each

    Journal: BMC Microbiology

    Article Title: Isothermal microcalorimetry for thermal viable count of microorganisms in pure cultures and stabilized formulations

    doi: 10.1186/s12866-019-1432-8

    Figure Lengend Snippet: Isothermal microcalorimetry thermal power curves (μW) and respective initial microbial cell densities for each curve (log (cfu/ml)). The pure culture volume was 6 ml, and the seed sample size was 3 g, supplemented with 5.5 ml of media. a Pseudomonas brassicacearum MA250 pure culture in TSB, 25 °C. b P. brassicacearum MA250 coated on wheat seeds, 25 °C. C = surface sterilization control (3.1 log (cfu/ml)). c Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. d Clonostachys rosea IK726 in PDB, 25 °C. Each strain displayed distinct and repeatable thermal power curves. The figures represent the averages and standard deviations of 2–3 independent experiments with 3–6 replicates each

    Article Snippet: P. brassicacearum MA250 cells were cultured in tryptic soy broth (TSB) (105,459, Merck, Germany) with shaking at 210 rpm or on TSA (TSB + agar) (105,458, Merck, Germany) at 25 °C.

    Techniques:

    Isothermal microcalorimetry thermal power (μW) curves of microbes in different culturing volumes (3, 6, 12 and 18 ml). a Pseudomonas brassicacearum MA250 in TSB, 25 °C. b Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. c Clonostachys rosea IK726 in PDB, 25 °C. The figures for Pseudomonas and Bacillus represent the averages and standard deviations of three independent experiments with 3–6 replicates per treatment. The figure for C. rosea IK726 represents the averages and standard deviations of six replicates per treatment

    Journal: BMC Microbiology

    Article Title: Isothermal microcalorimetry for thermal viable count of microorganisms in pure cultures and stabilized formulations

    doi: 10.1186/s12866-019-1432-8

    Figure Lengend Snippet: Isothermal microcalorimetry thermal power (μW) curves of microbes in different culturing volumes (3, 6, 12 and 18 ml). a Pseudomonas brassicacearum MA250 in TSB, 25 °C. b Bacillus amyloliquefaciens subsp. plantarum UCMB5113 in LB, 25 °C. c Clonostachys rosea IK726 in PDB, 25 °C. The figures for Pseudomonas and Bacillus represent the averages and standard deviations of three independent experiments with 3–6 replicates per treatment. The figure for C. rosea IK726 represents the averages and standard deviations of six replicates per treatment

    Article Snippet: P. brassicacearum MA250 cells were cultured in tryptic soy broth (TSB) (105,459, Merck, Germany) with shaking at 210 rpm or on TSA (TSB + agar) (105,458, Merck, Germany) at 25 °C.

    Techniques:

    S. epidermidis bacteria obtained from high dormancy biofilms induce a lower activation of murine BMDCs. Cell suspensions obtained from 48 h biofilms grown in TSB 1 %G (dormant biofilms), 48 h biofilms grown in TSB 1 %G+Mg 2+ (non-dormant biofilms) or original planktonic cultures (planktonic) were used to stimulate BMDCs in vitro . The concentration of the proinflammatory cytokines (a) TNF-α, (b) IL-12 and (c) IL-6, and of anti-inflammatory cytokine (d) IL-10, was evaluated by ELISA in the culture supernatants. Results shown for each cytokine are representative of two independent experiments that generated concordant results. Statistical analysis was carried out by one-way ANOVA with Tukey's multiple comparison test.

    Journal: Journal of Medical Microbiology

    Article Title: Dormant bacteria within Staphylococcus epidermidis biofilms have low inflammatory properties and maintain tolerance to vancomycin and penicillin after entering planktonic growth

    doi: 10.1099/jmm.0.073163-0

    Figure Lengend Snippet: S. epidermidis bacteria obtained from high dormancy biofilms induce a lower activation of murine BMDCs. Cell suspensions obtained from 48 h biofilms grown in TSB 1 %G (dormant biofilms), 48 h biofilms grown in TSB 1 %G+Mg 2+ (non-dormant biofilms) or original planktonic cultures (planktonic) were used to stimulate BMDCs in vitro . The concentration of the proinflammatory cytokines (a) TNF-α, (b) IL-12 and (c) IL-6, and of anti-inflammatory cytokine (d) IL-10, was evaluated by ELISA in the culture supernatants. Results shown for each cytokine are representative of two independent experiments that generated concordant results. Statistical analysis was carried out by one-way ANOVA with Tukey's multiple comparison test.

    Article Snippet: To establish a 48 h biofilm, a starter culture was grown overnight in tryptic soy broth (TSB) (Merck) at 37 °C with agitation (80 r.p.m.).

    Techniques: Activation Assay, In Vitro, Concentration Assay, Enzyme-linked Immunosorbent Assay, Generated

    Evaluation of icaA and rpiA gene expression over time in S. epidermidis bacteria grown in the biofilm or planktonic modes. Bacteria obtained from low dormancy 48 h S. epidermidis biofilms were diluted in fresh TSB (1×10 8 cells ml −1 ) and allowed to grow in the planktonic form for a 6 h period. Simultaneously, parallel cultures of 48 h S. epidermidis biofilms were allowed to grow for an additional 6 h period in fresh TSB. At the indicated time points, a sample of bacteria was obtained from each culture and the expression of (a) icaA and (b) rpiA genes was evaluated by qPCR. Results shown are representative of two independent experiments that generated concordant results. Statistical analysis was carried out by two-way repeated-measures ANOVA with Bonferroni post hoc test.

    Journal: Journal of Medical Microbiology

    Article Title: Dormant bacteria within Staphylococcus epidermidis biofilms have low inflammatory properties and maintain tolerance to vancomycin and penicillin after entering planktonic growth

    doi: 10.1099/jmm.0.073163-0

    Figure Lengend Snippet: Evaluation of icaA and rpiA gene expression over time in S. epidermidis bacteria grown in the biofilm or planktonic modes. Bacteria obtained from low dormancy 48 h S. epidermidis biofilms were diluted in fresh TSB (1×10 8 cells ml −1 ) and allowed to grow in the planktonic form for a 6 h period. Simultaneously, parallel cultures of 48 h S. epidermidis biofilms were allowed to grow for an additional 6 h period in fresh TSB. At the indicated time points, a sample of bacteria was obtained from each culture and the expression of (a) icaA and (b) rpiA genes was evaluated by qPCR. Results shown are representative of two independent experiments that generated concordant results. Statistical analysis was carried out by two-way repeated-measures ANOVA with Bonferroni post hoc test.

    Article Snippet: To establish a 48 h biofilm, a starter culture was grown overnight in tryptic soy broth (TSB) (Merck) at 37 °C with agitation (80 r.p.m.).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Generated

    Dormant bacteria within S. epidermidis biofilms maintain tolerance to vancomycin and penicillin after initiating planktonic growth. (a) Cell suspensions obtained from biofilms with high proportions of dormant bacteria were diluted in fresh TSB (1×10 8 cells ml −1 ) and allowed to grow for a further 6 h period in the planktonic form. At this time point, the bacterial SYBR staining intensity was determined by FACS analysis. (b) Vancomycin (40 µg ml −1 ), penicillin (40 µg ml −1 ) or TSB was added to the 6 h planktonic cultures, which were incubated for a period of 30 min. Bacterial death was determined by flow cytometry analysis assessing incorporation of PI. Results shown are representative of two independent experiments that generated concordant results. Statistically significant differences in the proportions of SYBR high PI + bacteria (gate g4) between control, vancomycin and penicillin groups ( P

    Journal: Journal of Medical Microbiology

    Article Title: Dormant bacteria within Staphylococcus epidermidis biofilms have low inflammatory properties and maintain tolerance to vancomycin and penicillin after entering planktonic growth

    doi: 10.1099/jmm.0.073163-0

    Figure Lengend Snippet: Dormant bacteria within S. epidermidis biofilms maintain tolerance to vancomycin and penicillin after initiating planktonic growth. (a) Cell suspensions obtained from biofilms with high proportions of dormant bacteria were diluted in fresh TSB (1×10 8 cells ml −1 ) and allowed to grow for a further 6 h period in the planktonic form. At this time point, the bacterial SYBR staining intensity was determined by FACS analysis. (b) Vancomycin (40 µg ml −1 ), penicillin (40 µg ml −1 ) or TSB was added to the 6 h planktonic cultures, which were incubated for a period of 30 min. Bacterial death was determined by flow cytometry analysis assessing incorporation of PI. Results shown are representative of two independent experiments that generated concordant results. Statistically significant differences in the proportions of SYBR high PI + bacteria (gate g4) between control, vancomycin and penicillin groups ( P

    Article Snippet: To establish a 48 h biofilm, a starter culture was grown overnight in tryptic soy broth (TSB) (Merck) at 37 °C with agitation (80 r.p.m.).

    Techniques: Staining, FACS, Incubation, Flow Cytometry, Cytometry, Generated

    S. epidermidis biofilm bacteria acquire susceptibility to vancomycin and penicillin after initiating a planktonic growth. Bacteria obtained from low dormancy 48 h S. epidermidis biofilms were diluted in fresh TSB (1×10 8 cells ml −1 ) and allowed to grow in the planktonic form for a 3 h period. Simultaneously, parallel cultures of 48 h S. epidermidis biofilms were allowed to grow for an additional 3 h period in fresh TSB. At this time point, vancomycin (40 μ g ml −1 ), penicillin (40 μ g ml −1 ) or TSB (negative control) was added to the biofilm and planktonic cultures, which were grown for an additional 30 min. Bacterial death was determined using flow cytometry by assessing the bacterial incorporation of PI. Results shown are representative of two independent experiments that generated concordant results. Statistically significant differences in the proportions of dead PI + bacteria (gate g2) between control, vancomycin and penicillin groups ( P

    Journal: Journal of Medical Microbiology

    Article Title: Dormant bacteria within Staphylococcus epidermidis biofilms have low inflammatory properties and maintain tolerance to vancomycin and penicillin after entering planktonic growth

    doi: 10.1099/jmm.0.073163-0

    Figure Lengend Snippet: S. epidermidis biofilm bacteria acquire susceptibility to vancomycin and penicillin after initiating a planktonic growth. Bacteria obtained from low dormancy 48 h S. epidermidis biofilms were diluted in fresh TSB (1×10 8 cells ml −1 ) and allowed to grow in the planktonic form for a 3 h period. Simultaneously, parallel cultures of 48 h S. epidermidis biofilms were allowed to grow for an additional 3 h period in fresh TSB. At this time point, vancomycin (40 μ g ml −1 ), penicillin (40 μ g ml −1 ) or TSB (negative control) was added to the biofilm and planktonic cultures, which were grown for an additional 30 min. Bacterial death was determined using flow cytometry by assessing the bacterial incorporation of PI. Results shown are representative of two independent experiments that generated concordant results. Statistically significant differences in the proportions of dead PI + bacteria (gate g2) between control, vancomycin and penicillin groups ( P

    Article Snippet: To establish a 48 h biofilm, a starter culture was grown overnight in tryptic soy broth (TSB) (Merck) at 37 °C with agitation (80 r.p.m.).

    Techniques: Negative Control, Flow Cytometry, Cytometry, Generated

    Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus HG003 in TSB supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.

    Journal: mBio

    Article Title: Acetic Acid Acts as a Volatile Signal To Stimulate Bacterial Biofilm Formation

    doi: 10.1128/mBio.00392-15

    Figure Lengend Snippet: Acetate salt stimulates biofilm formation in S. aureus . (A) Formation of submerged, surface-attached biofilms by S. aureus HG003 in TSB supplemented with 1% glucose in the absence or presence of 0.2%, 0.5%, and 1% NaAc. A concentration of 1% is defined as 10 g of NaAc in 1 liter of medium. Biofilms attached to the bottom of the 24-well polyvinyl plate (VWR) were visualized by crystal violet staining. (B) Results of a semiquantitative measurement to compare the biomasses of the surface-attached biofilms described for panel A. AU, absorbance units.

    Article Snippet: To do so, HG003 cells were inoculated in tryptic soy broth (TSB) supplemented with 1% glucose in 24-well polyvinyl plates (VWR) to allow formation of surface-attached biofilms (see Materials and Methods).

    Techniques: Concentration Assay, Staining

    Soluble thiols analysis. HPLC analysis of nonprotein thiols in S. maltophilia Sm777 grown in TSB/10 without (A) or supplemented (B) with 500 µM of CdCl 2 . The arrow indicates cysteine peack. N -acetyl-L-cysteine (NAC) was used as an internal standard.

    Journal: PLoS ONE

    Article Title: Heavy Metal Tolerance in Stenotrophomonas maltophilia

    doi: 10.1371/journal.pone.0001539

    Figure Lengend Snippet: Soluble thiols analysis. HPLC analysis of nonprotein thiols in S. maltophilia Sm777 grown in TSB/10 without (A) or supplemented (B) with 500 µM of CdCl 2 . The arrow indicates cysteine peack. N -acetyl-L-cysteine (NAC) was used as an internal standard.

    Article Snippet: Growth conditions S. maltophilia Sm777 was grown aerobically in an incubating shaker at 30°C in tenfold diluted tryptic soy broth (TSB/10) (DIFCO Laboratories, Detroit, USA).

    Techniques: High Performance Liquid Chromatography