trypsin-edta Search Results


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  • 99
    Lonza trypsin ethylenediaminetetraacetic acid
    Trypsin Ethylenediaminetetraacetic Acid, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC trypsin edta
    Trypsin Edta, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 343 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trypsin edta
    Trypsin Edta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 51633 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore trypsin edta
    Trypsin Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trypsin edta solution
    Trypsin Edta Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore trypsin edta solution
    Trypsin Edta Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3526 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trypsin ethylenediaminetetraacetic acid edta
    Trypsin Ethylenediaminetetraacetic Acid Edta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 816 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Corning Life Sciences trypsin edta
    Trypsin Edta, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 861 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech trypsin edta
    Trypsin Edta, supplied by Mediatech, used in various techniques. Bioz Stars score: 92/100, based on 941 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mscs  (Lonza)
    96
    Lonza mscs
    Mscs, supplied by Lonza, used in various techniques. Bioz Stars score: 96/100, based on 1176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biochrom trypsin edta
    Trypsin Edta, supplied by Biochrom, used in various techniques. Bioz Stars score: 92/100, based on 667 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare trypsin edta
    Trypsin Edta, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 98/100, based on 1396 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PAA Laboratories trypsin edta
    Trypsin Edta, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 830 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellgro trypsin edta
    Trypsin Edta, supplied by Cellgro, used in various techniques. Bioz Stars score: 92/100, based on 510 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare trypsin
    Trypsin, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1675 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson trypsin edta
    Knockdown of P-gp in 4T1-R (top panel), SKBR-3 (middle panel) and MCF-7 (bottom panel) breast cancer cells with/without aptamer-labeled F31 nanoparticles compared to lipofectamine transfection. In both cases of lipofectamine and aptamer-labeled nanoparticle transfection, the cells were transfected with 100 pmol siRNA for 24h, and then the cells were scraped by using <t>trypsin-EDTA,</t> washed with <t>PBS,</t> pelleted and the expression of P-gp was measured by Western blot analysis.
    Trypsin Edta, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 2166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Lonza trypsin edta solution
    Knockdown of P-gp in 4T1-R (top panel), SKBR-3 (middle panel) and MCF-7 (bottom panel) breast cancer cells with/without aptamer-labeled F31 nanoparticles compared to lipofectamine transfection. In both cases of lipofectamine and aptamer-labeled nanoparticle transfection, the cells were transfected with 100 pmol siRNA for 24h, and then the cells were scraped by using <t>trypsin-EDTA,</t> washed with <t>PBS,</t> pelleted and the expression of P-gp was measured by Western blot analysis.
    Trypsin Edta Solution, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher edta trypsin
    Knockdown of P-gp in 4T1-R (top panel), SKBR-3 (middle panel) and MCF-7 (bottom panel) breast cancer cells with/without aptamer-labeled F31 nanoparticles compared to lipofectamine transfection. In both cases of lipofectamine and aptamer-labeled nanoparticle transfection, the cells were transfected with 100 pmol siRNA for 24h, and then the cells were scraped by using <t>trypsin-EDTA,</t> washed with <t>PBS,</t> pelleted and the expression of P-gp was measured by Western blot analysis.
    Edta Trypsin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Thermo Fisher trypsin edta 1x
    Knockdown of P-gp in 4T1-R (top panel), SKBR-3 (middle panel) and MCF-7 (bottom panel) breast cancer cells with/without aptamer-labeled F31 nanoparticles compared to lipofectamine transfection. In both cases of lipofectamine and aptamer-labeled nanoparticle transfection, the cells were transfected with 100 pmol siRNA for 24h, and then the cells were scraped by using <t>trypsin-EDTA,</t> washed with <t>PBS,</t> pelleted and the expression of P-gp was measured by Western blot analysis.
    Trypsin Edta 1x, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 355 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Welgene inc trypsin edta
    Knockdown of P-gp in 4T1-R (top panel), SKBR-3 (middle panel) and MCF-7 (bottom panel) breast cancer cells with/without aptamer-labeled F31 nanoparticles compared to lipofectamine transfection. In both cases of lipofectamine and aptamer-labeled nanoparticle transfection, the cells were transfected with 100 pmol siRNA for 24h, and then the cells were scraped by using <t>trypsin-EDTA,</t> washed with <t>PBS,</t> pelleted and the expression of P-gp was measured by Western blot analysis.
    Trypsin Edta, supplied by Welgene inc, used in various techniques. Bioz Stars score: 92/100, based on 240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Biological Industries Inc trypsin edta
    Knockdown of P-gp in 4T1-R (top panel), SKBR-3 (middle panel) and MCF-7 (bottom panel) breast cancer cells with/without aptamer-labeled F31 nanoparticles compared to lipofectamine transfection. In both cases of lipofectamine and aptamer-labeled nanoparticle transfection, the cells were transfected with 100 pmol siRNA for 24h, and then the cells were scraped by using <t>trypsin-EDTA,</t> washed with <t>PBS,</t> pelleted and the expression of P-gp was measured by Western blot analysis.
    Trypsin Edta, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 92/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    edta  (ATCC)
    99
    ATCC edta
    Knockdown of P-gp in 4T1-R (top panel), SKBR-3 (middle panel) and MCF-7 (bottom panel) breast cancer cells with/without aptamer-labeled F31 nanoparticles compared to lipofectamine transfection. In both cases of lipofectamine and aptamer-labeled nanoparticle transfection, the cells were transfected with 100 pmol siRNA for 24h, and then the cells were scraped by using <t>trypsin-EDTA,</t> washed with <t>PBS,</t> pelleted and the expression of P-gp was measured by Western blot analysis.
    Edta, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Knockdown of P-gp in 4T1-R (top panel), SKBR-3 (middle panel) and MCF-7 (bottom panel) breast cancer cells with/without aptamer-labeled F31 nanoparticles compared to lipofectamine transfection. In both cases of lipofectamine and aptamer-labeled nanoparticle transfection, the cells were transfected with 100 pmol siRNA for 24h, and then the cells were scraped by using trypsin-EDTA, washed with PBS, pelleted and the expression of P-gp was measured by Western blot analysis.

    Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

    Article Title: Aptamer-functionalized hybrid nanoparticle for the treatment of breast cancer

    doi: 10.1016/j.ejpb.2017.01.011

    Figure Lengend Snippet: Knockdown of P-gp in 4T1-R (top panel), SKBR-3 (middle panel) and MCF-7 (bottom panel) breast cancer cells with/without aptamer-labeled F31 nanoparticles compared to lipofectamine transfection. In both cases of lipofectamine and aptamer-labeled nanoparticle transfection, the cells were transfected with 100 pmol siRNA for 24h, and then the cells were scraped by using trypsin-EDTA, washed with PBS, pelleted and the expression of P-gp was measured by Western blot analysis.

    Article Snippet: After treatment, the cells were scraped by using trypsin-EDTA, washed and resuspended in PBS and kept on ice until they were used to quantify the uptake of the nanoparticles by those cells by FACS (BD FACSCalibur).

    Techniques: Labeling, Transfection, Expressing, Western Blot

    Measurement of the expression of Her-2 (A.) and P-gp (B.) in different mouse ( i.e. 4T1-R and 4T1-S) and human breast cancer cells ( i.e. SKBR-3, MCF-7 and MDA MB-231) and human liver cancer cells ( i.e. Huh-7.5 and HepG2) by Western blot analysis. The cells were cultured for 24h and then they were scraped by using trypsin-EDTA, washed with PBS, pelleted and then equal amount of proteins was loaded to measure the expression of Her-2 (1:1000 dilution) and P-gp (1:1000 dilution) with β-actin (1:20,000 dilution) running as the loading control.

    Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

    Article Title: Aptamer-functionalized hybrid nanoparticle for the treatment of breast cancer

    doi: 10.1016/j.ejpb.2017.01.011

    Figure Lengend Snippet: Measurement of the expression of Her-2 (A.) and P-gp (B.) in different mouse ( i.e. 4T1-R and 4T1-S) and human breast cancer cells ( i.e. SKBR-3, MCF-7 and MDA MB-231) and human liver cancer cells ( i.e. Huh-7.5 and HepG2) by Western blot analysis. The cells were cultured for 24h and then they were scraped by using trypsin-EDTA, washed with PBS, pelleted and then equal amount of proteins was loaded to measure the expression of Her-2 (1:1000 dilution) and P-gp (1:1000 dilution) with β-actin (1:20,000 dilution) running as the loading control.

    Article Snippet: After treatment, the cells were scraped by using trypsin-EDTA, washed and resuspended in PBS and kept on ice until they were used to quantify the uptake of the nanoparticles by those cells by FACS (BD FACSCalibur).

    Techniques: Expressing, Multiple Displacement Amplification, Western Blot, Cell Culture