Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

Article Title: Aptamer-functionalized hybrid nanoparticle for the treatment of breast cancer

doi: 10.1016/j.ejpb.2017.01.011

Figure Lengend Snippet: Knockdown of P-gp in 4T1-R (top panel), SKBR-3 (middle panel) and MCF-7 (bottom panel) breast cancer cells with/without aptamer-labeled F31 nanoparticles compared to lipofectamine transfection. In both cases of lipofectamine and aptamer-labeled nanoparticle transfection, the cells were transfected with 100 pmol siRNA for 24h, and then the cells were scraped by using trypsin-EDTA, washed with PBS, pelleted and the expression of P-gp was measured by Western blot analysis.

Article Snippet: After treatment, the cells were scraped by using trypsin-EDTA, washed and resuspended in PBS and kept on ice until they were used to quantify the uptake of the nanoparticles by those cells by FACS (BD FACSCalibur).

Techniques: Labeling, Transfection, Expressing, Western Blot

Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

Article Title: Aptamer-functionalized hybrid nanoparticle for the treatment of breast cancer

doi: 10.1016/j.ejpb.2017.01.011

Figure Lengend Snippet: Measurement of the expression of Her-2 (A.) and P-gp (B.) in different mouse ( i.e. 4T1-R and 4T1-S) and human breast cancer cells ( i.e. SKBR-3, MCF-7 and MDA MB-231) and human liver cancer cells ( i.e. Huh-7.5 and HepG2) by Western blot analysis. The cells were cultured for 24h and then they were scraped by using trypsin-EDTA, washed with PBS, pelleted and then equal amount of proteins was loaded to measure the expression of Her-2 (1:1000 dilution) and P-gp (1:1000 dilution) with β-actin (1:20,000 dilution) running as the loading control.

Article Snippet: After treatment, the cells were scraped by using trypsin-EDTA, washed and resuspended in PBS and kept on ice until they were used to quantify the uptake of the nanoparticles by those cells by FACS (BD FACSCalibur).

Techniques: Expressing, Multiple Displacement Amplification, Western Blot, Cell Culture