trypsin-edta Search Results


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  • 99
    Thermo Fisher trypsinedta
    Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with <t>trypsin-EDTA,</t> re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.
    Trypsinedta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore trypsinedta
    Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with <t>trypsin-EDTA,</t> re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.
    Trypsinedta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Lonza trypsin edta
    Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with <t>trypsin-EDTA,</t> re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.
    Trypsin Edta, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 1225 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Amresco trypsin edta
    Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with <t>trypsin-EDTA,</t> re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.
    Trypsin Edta, supplied by Amresco, used in various techniques. Bioz Stars score: 93/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Beijing Solarbio Science trypsin edta
    Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with <t>trypsin-EDTA,</t> re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.
    Trypsin Edta, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Beyotime trypsin edta
    Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with <t>trypsin-EDTA,</t> re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.
    Trypsin Edta, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Biochrom trypsin edta
    Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with <t>trypsin-EDTA,</t> re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.
    Trypsin Edta, supplied by Biochrom, used in various techniques. Bioz Stars score: 92/100, based on 537 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Biological Industries Inc trypsin edta
    Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with <t>trypsin-EDTA,</t> re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.
    Trypsin Edta, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 93/100, based on 187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Biowest SAS trypsin edta
    Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with <t>trypsin-EDTA,</t> re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.
    Trypsin Edta, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 94/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cell Applications Inc trypsin edta
    Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with <t>trypsin-EDTA,</t> re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.
    Trypsin Edta, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellgro trypsin edta
    Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with <t>trypsin-EDTA,</t> re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.
    Trypsin Edta, supplied by Cellgro, used in various techniques. Bioz Stars score: 92/100, based on 362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Corning Life Sciences trypsin edta
    Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with <t>trypsin-EDTA,</t> re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.
    Trypsin Edta, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 96/100, based on 762 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Cytogen trypsin edta
    Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with <t>trypsin-EDTA,</t> re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.
    Trypsin Edta, supplied by Cytogen, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    EuroClone trypsin edta
    Expression and localization of cleaved and secreted form of ANXA1 from <t>MIA</t> PaCa-2 and PANC-1 cells. Cellular compartments were obtained as described in Methods section. Total (T), membrane (M), <t>EDTA</t> Wash (EW), cytosolic (C), nuclear (N) and supernatant (S) ANXA1 expression in protein extracts from MIA PaCa-2 and PANC-1 was examined by Western blot with anti-ANXA1 antibody. The protein bands were normalized on tubulin levels. The data are representative of 5 experiments with similar results.
    Trypsin Edta, supplied by EuroClone, used in various techniques. Bioz Stars score: 93/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Scientific trypsin edta
    Expression and localization of cleaved and secreted form of ANXA1 from <t>MIA</t> PaCa-2 and PANC-1 cells. Cellular compartments were obtained as described in Methods section. Total (T), membrane (M), <t>EDTA</t> Wash (EW), cytosolic (C), nuclear (N) and supernatant (S) ANXA1 expression in protein extracts from MIA PaCa-2 and PANC-1 was examined by Western blot with anti-ANXA1 antibody. The protein bands were normalized on tubulin levels. The data are representative of 5 experiments with similar results.
    Trypsin Edta, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GE Healthcare trypsin ethylenediaminetetraacetic acid
    Expression and localization of cleaved and secreted form of ANXA1 from <t>MIA</t> PaCa-2 and PANC-1 cells. Cellular compartments were obtained as described in Methods section. Total (T), membrane (M), <t>EDTA</t> Wash (EW), cytosolic (C), nuclear (N) and supernatant (S) ANXA1 expression in protein extracts from MIA PaCa-2 and PANC-1 was examined by Western blot with anti-ANXA1 antibody. The protein bands were normalized on tubulin levels. The data are representative of 5 experiments with similar results.
    Trypsin Ethylenediaminetetraacetic Acid, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Gemini Bio trypsin edta
    Expression and localization of cleaved and secreted form of ANXA1 from <t>MIA</t> PaCa-2 and PANC-1 cells. Cellular compartments were obtained as described in Methods section. Total (T), membrane (M), <t>EDTA</t> Wash (EW), cytosolic (C), nuclear (N) and supernatant (S) ANXA1 expression in protein extracts from MIA PaCa-2 and PANC-1 was examined by Western blot with anti-ANXA1 antibody. The protein bands were normalized on tubulin levels. The data are representative of 5 experiments with similar results.
    Trypsin Edta, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 91/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Irvine Scientific trypsin edta
    Expression and localization of cleaved and secreted form of ANXA1 from <t>MIA</t> PaCa-2 and PANC-1 cells. Cellular compartments were obtained as described in Methods section. Total (T), membrane (M), <t>EDTA</t> Wash (EW), cytosolic (C), nuclear (N) and supernatant (S) ANXA1 expression in protein extracts from MIA PaCa-2 and PANC-1 was examined by Western blot with anti-ANXA1 antibody. The protein bands were normalized on tubulin levels. The data are representative of 5 experiments with similar results.
    Trypsin Edta, supplied by Irvine Scientific, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Keygen Biotech trypsin edta
    Expression and localization of cleaved and secreted form of ANXA1 from <t>MIA</t> PaCa-2 and PANC-1 cells. Cellular compartments were obtained as described in Methods section. Total (T), membrane (M), <t>EDTA</t> Wash (EW), cytosolic (C), nuclear (N) and supernatant (S) ANXA1 expression in protein extracts from MIA PaCa-2 and PANC-1 was examined by Western blot with anti-ANXA1 antibody. The protein bands were normalized on tubulin levels. The data are representative of 5 experiments with similar results.
    Trypsin Edta, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech trypsin edta
    Expression and localization of cleaved and secreted form of ANXA1 from <t>MIA</t> PaCa-2 and PANC-1 cells. Cellular compartments were obtained as described in Methods section. Total (T), membrane (M), <t>EDTA</t> Wash (EW), cytosolic (C), nuclear (N) and supernatant (S) ANXA1 expression in protein extracts from MIA PaCa-2 and PANC-1 was examined by Western blot with anti-ANXA1 antibody. The protein bands were normalized on tubulin levels. The data are representative of 5 experiments with similar results.
    Trypsin Edta, supplied by Mediatech, used in various techniques. Bioz Stars score: 92/100, based on 799 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Melone Pharmaceutical Co Ltd trypsin edta
    Expression and localization of cleaved and secreted form of ANXA1 from <t>MIA</t> PaCa-2 and PANC-1 cells. Cellular compartments were obtained as described in Methods section. Total (T), membrane (M), <t>EDTA</t> Wash (EW), cytosolic (C), nuclear (N) and supernatant (S) ANXA1 expression in protein extracts from MIA PaCa-2 and PANC-1 was examined by Western blot with anti-ANXA1 antibody. The protein bands were normalized on tubulin levels. The data are representative of 5 experiments with similar results.
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    Image Search Results


    Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with trypsin-EDTA, re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.

    Journal: Scientific Reports

    Article Title: Preservation of epithelial progenitor cells from collagenase-digested oral mucosa during ex vivo cultivation

    doi: 10.1038/srep36266

    Figure Lengend Snippet: Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs). Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with trypsin-EDTA, re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.

    Article Snippet: Materials DMEM:F12 (1:1), Opti-MEM, EpiLife medium, , trypsin-EDTA, phosphate-buffered saline (PBS), gentamicin, amphotericin B, Lipofectamine 2000 and Alexa-Fluor-conjugated secondary IgG were purchased from Invitrogen (Carlsbad, CA).

    Techniques: Modification, Ex Vivo, Isolation

    Expression and localization of cleaved and secreted form of ANXA1 from MIA PaCa-2 and PANC-1 cells. Cellular compartments were obtained as described in Methods section. Total (T), membrane (M), EDTA Wash (EW), cytosolic (C), nuclear (N) and supernatant (S) ANXA1 expression in protein extracts from MIA PaCa-2 and PANC-1 was examined by Western blot with anti-ANXA1 antibody. The protein bands were normalized on tubulin levels. The data are representative of 5 experiments with similar results.

    Journal: BMC Cancer

    Article Title: Role of intracellular and extracellular annexin A1 in migration and invasion of human pancreatic carcinoma cells

    doi: 10.1186/1471-2407-14-961

    Figure Lengend Snippet: Expression and localization of cleaved and secreted form of ANXA1 from MIA PaCa-2 and PANC-1 cells. Cellular compartments were obtained as described in Methods section. Total (T), membrane (M), EDTA Wash (EW), cytosolic (C), nuclear (N) and supernatant (S) ANXA1 expression in protein extracts from MIA PaCa-2 and PANC-1 was examined by Western blot with anti-ANXA1 antibody. The protein bands were normalized on tubulin levels. The data are representative of 5 experiments with similar results.

    Article Snippet: Nuclear extracts MIA PaCa-2 and PANC-1 cells were washed twice with PBS, detached with trypsin-EDTA 1× in PBS (Euroclone), harvested in PBS and centrifuged for 5 minutes at 600 × g at 4°C.

    Techniques: Expressing, Western Blot