trypsin inhibitor Search Results


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  • 99
    Worthington Biochemical soybean trypsin inhibitor
    Soybean Trypsin Inhibitor, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 549 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 549 article reviews
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    96
    Thermo Fisher trypsin inhibitor
    Trypsin Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore trypsin inhibitor
    Trypsin Inhibitor, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5496 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher soybean trypsin inhibitor
    Soybean Trypsin Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 681 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Worthington Biochemical trypsin inhibitor
    Trypsin Inhibitor, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 209 article reviews
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    90
    Haematologic Technologies corn trypsin inhibitor
    Kallikrein-kinin system and BK inhibitors. A schematic illustration of three inhibitors that impact BK-mediated increase in endothelial cell permeability. <t>CTI</t> (corn trypsin inhibitor) inhibits FXIIa; <t>PKSI-527</t> (plasma kallikrein specific inhibitor) inhibits KAL activity; Icatibant (HOE 140) is a peptidomimetic drug consisting of ten amino acids, which is a selective and specific antagonist of BKB2 receptors and is used in humans under orphan drug status for symptomatic treatment of hereditary angioedema.
    Corn Trypsin Inhibitor, supplied by Haematologic Technologies, used in various techniques. Bioz Stars score: 90/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore soybean trypsin inhibitor sbti
    Kallikrein-kinin system and BK inhibitors. A schematic illustration of three inhibitors that impact BK-mediated increase in endothelial cell permeability. <t>CTI</t> (corn trypsin inhibitor) inhibits FXIIa; <t>PKSI-527</t> (plasma kallikrein specific inhibitor) inhibits KAL activity; Icatibant (HOE 140) is a peptidomimetic drug consisting of ten amino acids, which is a selective and specific antagonist of BKB2 receptors and is used in humans under orphan drug status for symptomatic treatment of hereditary angioedema.
    Soybean Trypsin Inhibitor Sbti, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3394 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore trypsin chymotrypsin inhibitor
    Kallikrein-kinin system and BK inhibitors. A schematic illustration of three inhibitors that impact BK-mediated increase in endothelial cell permeability. <t>CTI</t> (corn trypsin inhibitor) inhibits FXIIa; <t>PKSI-527</t> (plasma kallikrein specific inhibitor) inhibits KAL activity; Icatibant (HOE 140) is a peptidomimetic drug consisting of ten amino acids, which is a selective and specific antagonist of BKB2 receptors and is used in humans under orphan drug status for symptomatic treatment of hereditary angioedema.
    Trypsin Chymotrypsin Inhibitor, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Enzyme Research Laboratories corn trypsin inhibitor
    Detection of FXIa or kallikrein generation by <t>FXIIa</t> on the surface of HUVECs. ( A ) HK (100 nM) and FXI (100 nM) or ( B ) PK (100 nM) and HK (100 nM) were incubated with FXIIa (1 nM) for 1 hr in the presence ( middle panel ) or absence ( left panel ) of HUVECs. Reactions were stopped with <t>CTI</t> (40 μg/ml). FXIa and kallikrein activity was determined by adding S-2366 (0.8 mM) or S-2302 (0.6 mM), respectively; the rate of substrate hydrolysis was measured at 405 nm. Empty wells or HUVECs were incubated with increasing concentrations of ( A, right panel ) FXIa or ( B, right panel ) kallikrein for 2 hrs at 37°C FXIa and kallikrein activity was determined by adding S-2366 (0.8 mM) or S2302 (0.6 mM), respectively and the rate of substrate hydrolysis was measured at 405 nm. ( C ) HUVECs were incubated for 16 hrs in the absence (□) or presence of TNFα (0.5 ng/ml) (○) or incubated for 4 hrs with elastase (50 nM) (△). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366 (0.6 mM) and the rate of substrate hydrolysis was measured at 405 nm. ( D ) HUVECs were incubated for 3 hrs in the absence or presence of heparinase I, II and III (1 U/ml) followed by cell surface detection of heparan sulfate using an anti-10E4 epitope antibody. ( E ) HUVECs were incubated for 3 hrs in the absence (□) or presence (○) of heparinase I, II and III (1 U/ml). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366. ( F ) HUVECs were incubated for 4 hrs in the absence (□) or presence of thrombin (20 nM) (○). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366. Data are mean ± SD (n = 3). * Indicates between-groups differences with P
    Corn Trypsin Inhibitor, supplied by Enzyme Research Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 76 article reviews
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    92
    Boehringer Mannheim soybean trypsin inhibitor
    Detection of FXIa or kallikrein generation by <t>FXIIa</t> on the surface of HUVECs. ( A ) HK (100 nM) and FXI (100 nM) or ( B ) PK (100 nM) and HK (100 nM) were incubated with FXIIa (1 nM) for 1 hr in the presence ( middle panel ) or absence ( left panel ) of HUVECs. Reactions were stopped with <t>CTI</t> (40 μg/ml). FXIa and kallikrein activity was determined by adding S-2366 (0.8 mM) or S-2302 (0.6 mM), respectively; the rate of substrate hydrolysis was measured at 405 nm. Empty wells or HUVECs were incubated with increasing concentrations of ( A, right panel ) FXIa or ( B, right panel ) kallikrein for 2 hrs at 37°C FXIa and kallikrein activity was determined by adding S-2366 (0.8 mM) or S2302 (0.6 mM), respectively and the rate of substrate hydrolysis was measured at 405 nm. ( C ) HUVECs were incubated for 16 hrs in the absence (□) or presence of TNFα (0.5 ng/ml) (○) or incubated for 4 hrs with elastase (50 nM) (△). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366 (0.6 mM) and the rate of substrate hydrolysis was measured at 405 nm. ( D ) HUVECs were incubated for 3 hrs in the absence or presence of heparinase I, II and III (1 U/ml) followed by cell surface detection of heparan sulfate using an anti-10E4 epitope antibody. ( E ) HUVECs were incubated for 3 hrs in the absence (□) or presence (○) of heparinase I, II and III (1 U/ml). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366. ( F ) HUVECs were incubated for 4 hrs in the absence (□) or presence of thrombin (20 nM) (○). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366. Data are mean ± SD (n = 3). * Indicates between-groups differences with P
    Soybean Trypsin Inhibitor, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    FUJIFILM trypsin inhibitor
    Detection of FXIa or kallikrein generation by <t>FXIIa</t> on the surface of HUVECs. ( A ) HK (100 nM) and FXI (100 nM) or ( B ) PK (100 nM) and HK (100 nM) were incubated with FXIIa (1 nM) for 1 hr in the presence ( middle panel ) or absence ( left panel ) of HUVECs. Reactions were stopped with <t>CTI</t> (40 μg/ml). FXIa and kallikrein activity was determined by adding S-2366 (0.8 mM) or S-2302 (0.6 mM), respectively; the rate of substrate hydrolysis was measured at 405 nm. Empty wells or HUVECs were incubated with increasing concentrations of ( A, right panel ) FXIa or ( B, right panel ) kallikrein for 2 hrs at 37°C FXIa and kallikrein activity was determined by adding S-2366 (0.8 mM) or S2302 (0.6 mM), respectively and the rate of substrate hydrolysis was measured at 405 nm. ( C ) HUVECs were incubated for 16 hrs in the absence (□) or presence of TNFα (0.5 ng/ml) (○) or incubated for 4 hrs with elastase (50 nM) (△). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366 (0.6 mM) and the rate of substrate hydrolysis was measured at 405 nm. ( D ) HUVECs were incubated for 3 hrs in the absence or presence of heparinase I, II and III (1 U/ml) followed by cell surface detection of heparan sulfate using an anti-10E4 epitope antibody. ( E ) HUVECs were incubated for 3 hrs in the absence (□) or presence (○) of heparinase I, II and III (1 U/ml). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366. ( F ) HUVECs were incubated for 4 hrs in the absence (□) or presence of thrombin (20 nM) (○). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366. Data are mean ± SD (n = 3). * Indicates between-groups differences with P
    Trypsin Inhibitor, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Atlanta Biologicals soybean trypsin inhibitor
    Detection of FXIa or kallikrein generation by <t>FXIIa</t> on the surface of HUVECs. ( A ) HK (100 nM) and FXI (100 nM) or ( B ) PK (100 nM) and HK (100 nM) were incubated with FXIIa (1 nM) for 1 hr in the presence ( middle panel ) or absence ( left panel ) of HUVECs. Reactions were stopped with <t>CTI</t> (40 μg/ml). FXIa and kallikrein activity was determined by adding S-2366 (0.8 mM) or S-2302 (0.6 mM), respectively; the rate of substrate hydrolysis was measured at 405 nm. Empty wells or HUVECs were incubated with increasing concentrations of ( A, right panel ) FXIa or ( B, right panel ) kallikrein for 2 hrs at 37°C FXIa and kallikrein activity was determined by adding S-2366 (0.8 mM) or S2302 (0.6 mM), respectively and the rate of substrate hydrolysis was measured at 405 nm. ( C ) HUVECs were incubated for 16 hrs in the absence (□) or presence of TNFα (0.5 ng/ml) (○) or incubated for 4 hrs with elastase (50 nM) (△). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366 (0.6 mM) and the rate of substrate hydrolysis was measured at 405 nm. ( D ) HUVECs were incubated for 3 hrs in the absence or presence of heparinase I, II and III (1 U/ml) followed by cell surface detection of heparan sulfate using an anti-10E4 epitope antibody. ( E ) HUVECs were incubated for 3 hrs in the absence (□) or presence (○) of heparinase I, II and III (1 U/ml). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366. ( F ) HUVECs were incubated for 4 hrs in the absence (□) or presence of thrombin (20 nM) (○). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366. Data are mean ± SD (n = 3). * Indicates between-groups differences with P
    Soybean Trypsin Inhibitor, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Boehringer Mannheim trypsin inhibitor
    Detection of FXIa or kallikrein generation by <t>FXIIa</t> on the surface of HUVECs. ( A ) HK (100 nM) and FXI (100 nM) or ( B ) PK (100 nM) and HK (100 nM) were incubated with FXIIa (1 nM) for 1 hr in the presence ( middle panel ) or absence ( left panel ) of HUVECs. Reactions were stopped with <t>CTI</t> (40 μg/ml). FXIa and kallikrein activity was determined by adding S-2366 (0.8 mM) or S-2302 (0.6 mM), respectively; the rate of substrate hydrolysis was measured at 405 nm. Empty wells or HUVECs were incubated with increasing concentrations of ( A, right panel ) FXIa or ( B, right panel ) kallikrein for 2 hrs at 37°C FXIa and kallikrein activity was determined by adding S-2366 (0.8 mM) or S2302 (0.6 mM), respectively and the rate of substrate hydrolysis was measured at 405 nm. ( C ) HUVECs were incubated for 16 hrs in the absence (□) or presence of TNFα (0.5 ng/ml) (○) or incubated for 4 hrs with elastase (50 nM) (△). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366 (0.6 mM) and the rate of substrate hydrolysis was measured at 405 nm. ( D ) HUVECs were incubated for 3 hrs in the absence or presence of heparinase I, II and III (1 U/ml) followed by cell surface detection of heparan sulfate using an anti-10E4 epitope antibody. ( E ) HUVECs were incubated for 3 hrs in the absence (□) or presence (○) of heparinase I, II and III (1 U/ml). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366. ( F ) HUVECs were incubated for 4 hrs in the absence (□) or presence of thrombin (20 nM) (○). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366. Data are mean ± SD (n = 3). * Indicates between-groups differences with P
    Trypsin Inhibitor, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Kallikrein-kinin system and BK inhibitors. A schematic illustration of three inhibitors that impact BK-mediated increase in endothelial cell permeability. CTI (corn trypsin inhibitor) inhibits FXIIa; PKSI-527 (plasma kallikrein specific inhibitor) inhibits KAL activity; Icatibant (HOE 140) is a peptidomimetic drug consisting of ten amino acids, which is a selective and specific antagonist of BKB2 receptors and is used in humans under orphan drug status for symptomatic treatment of hereditary angioedema.

    Journal: PLoS Pathogens

    Article Title: Endothelial Cell Permeability during Hantavirus Infection Involves Factor XII-Dependent Increased Activation of the Kallikrein-Kinin System

    doi: 10.1371/journal.ppat.1003470

    Figure Lengend Snippet: Kallikrein-kinin system and BK inhibitors. A schematic illustration of three inhibitors that impact BK-mediated increase in endothelial cell permeability. CTI (corn trypsin inhibitor) inhibits FXIIa; PKSI-527 (plasma kallikrein specific inhibitor) inhibits KAL activity; Icatibant (HOE 140) is a peptidomimetic drug consisting of ten amino acids, which is a selective and specific antagonist of BKB2 receptors and is used in humans under orphan drug status for symptomatic treatment of hereditary angioedema.

    Article Snippet: PKSI-527 is a specific inhibitor of KAL activity (Enzo Life Sciences) and corn trypsin inhibitor (CTI) inhibits FXIIa (Haematologic Technologies Inc.).

    Techniques: Permeability, Activity Assay

    Kallikrein-kinin system activation and changes in permeability of hantavirus-infected HUVEC. Mock-, HTNV-, or ANDV-infected HUVEC were trypsinized, seeded onto ECIS chamberslides, and cultured until confluent. Media were removed and replaced with phenol red free EBM containing Zn 2+ . After an equilibration, 0.2 ml of phenol red free EBM containing Zn 2+ , FXII, PK, and HK (100 nM each) were added to cells (time zero) (black lines) (A). To measure inhibition of activation, some samples were treated with CTI (1 µM) (blue line), PKSI-527 (5 µM) (red line), or HOE 140 (1 µM) (green line) simultaneously with factors (B, C, D). Real time measurements frequency measurements were taken throughout the assay.

    Journal: PLoS Pathogens

    Article Title: Endothelial Cell Permeability during Hantavirus Infection Involves Factor XII-Dependent Increased Activation of the Kallikrein-Kinin System

    doi: 10.1371/journal.ppat.1003470

    Figure Lengend Snippet: Kallikrein-kinin system activation and changes in permeability of hantavirus-infected HUVEC. Mock-, HTNV-, or ANDV-infected HUVEC were trypsinized, seeded onto ECIS chamberslides, and cultured until confluent. Media were removed and replaced with phenol red free EBM containing Zn 2+ . After an equilibration, 0.2 ml of phenol red free EBM containing Zn 2+ , FXII, PK, and HK (100 nM each) were added to cells (time zero) (black lines) (A). To measure inhibition of activation, some samples were treated with CTI (1 µM) (blue line), PKSI-527 (5 µM) (red line), or HOE 140 (1 µM) (green line) simultaneously with factors (B, C, D). Real time measurements frequency measurements were taken throughout the assay.

    Article Snippet: PKSI-527 is a specific inhibitor of KAL activity (Enzo Life Sciences) and corn trypsin inhibitor (CTI) inhibits FXIIa (Haematologic Technologies Inc.).

    Techniques: Activation Assay, Permeability, Infection, Electric Cell-substrate Impedance Sensing, Cell Culture, Inhibition

    Detection of FXIa or kallikrein generation by FXIIa on the surface of HUVECs. ( A ) HK (100 nM) and FXI (100 nM) or ( B ) PK (100 nM) and HK (100 nM) were incubated with FXIIa (1 nM) for 1 hr in the presence ( middle panel ) or absence ( left panel ) of HUVECs. Reactions were stopped with CTI (40 μg/ml). FXIa and kallikrein activity was determined by adding S-2366 (0.8 mM) or S-2302 (0.6 mM), respectively; the rate of substrate hydrolysis was measured at 405 nm. Empty wells or HUVECs were incubated with increasing concentrations of ( A, right panel ) FXIa or ( B, right panel ) kallikrein for 2 hrs at 37°C FXIa and kallikrein activity was determined by adding S-2366 (0.8 mM) or S2302 (0.6 mM), respectively and the rate of substrate hydrolysis was measured at 405 nm. ( C ) HUVECs were incubated for 16 hrs in the absence (□) or presence of TNFα (0.5 ng/ml) (○) or incubated for 4 hrs with elastase (50 nM) (△). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366 (0.6 mM) and the rate of substrate hydrolysis was measured at 405 nm. ( D ) HUVECs were incubated for 3 hrs in the absence or presence of heparinase I, II and III (1 U/ml) followed by cell surface detection of heparan sulfate using an anti-10E4 epitope antibody. ( E ) HUVECs were incubated for 3 hrs in the absence (□) or presence (○) of heparinase I, II and III (1 U/ml). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366. ( F ) HUVECs were incubated for 4 hrs in the absence (□) or presence of thrombin (20 nM) (○). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366. Data are mean ± SD (n = 3). * Indicates between-groups differences with P

    Journal: Arteriosclerosis, thrombosis, and vascular biology

    Article Title: Endothelial plasminogen activator inhibitor-1 blocks the intrinsic pathway of coagulation, inducing the clearance and degradation of factor XIa.

    doi: 10.1161/ATVBAHA.119.312619

    Figure Lengend Snippet: Detection of FXIa or kallikrein generation by FXIIa on the surface of HUVECs. ( A ) HK (100 nM) and FXI (100 nM) or ( B ) PK (100 nM) and HK (100 nM) were incubated with FXIIa (1 nM) for 1 hr in the presence ( middle panel ) or absence ( left panel ) of HUVECs. Reactions were stopped with CTI (40 μg/ml). FXIa and kallikrein activity was determined by adding S-2366 (0.8 mM) or S-2302 (0.6 mM), respectively; the rate of substrate hydrolysis was measured at 405 nm. Empty wells or HUVECs were incubated with increasing concentrations of ( A, right panel ) FXIa or ( B, right panel ) kallikrein for 2 hrs at 37°C FXIa and kallikrein activity was determined by adding S-2366 (0.8 mM) or S2302 (0.6 mM), respectively and the rate of substrate hydrolysis was measured at 405 nm. ( C ) HUVECs were incubated for 16 hrs in the absence (□) or presence of TNFα (0.5 ng/ml) (○) or incubated for 4 hrs with elastase (50 nM) (△). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366 (0.6 mM) and the rate of substrate hydrolysis was measured at 405 nm. ( D ) HUVECs were incubated for 3 hrs in the absence or presence of heparinase I, II and III (1 U/ml) followed by cell surface detection of heparan sulfate using an anti-10E4 epitope antibody. ( E ) HUVECs were incubated for 3 hrs in the absence (□) or presence (○) of heparinase I, II and III (1 U/ml). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366. ( F ) HUVECs were incubated for 4 hrs in the absence (□) or presence of thrombin (20 nM) (○). Subsequently, cells were incubated with increasing concentrations of FXIa. FXIa activity was determined by adding S-2366. Data are mean ± SD (n = 3). * Indicates between-groups differences with P

    Article Snippet: Plasma-derived FXIIa, PK, kallikrein, HK and corn trypsin inhibitor (CTI) were from Enzyme Research Laboratories, Inc. (South Bend, IN, USA).

    Techniques: Incubation, Activity Assay

    Detection of FXIa activity in the presence of a blocking anti-PAI-1 antibody. ( A ) Empty wells (●, ○) or HUVECs (■, □) were incubated with FXIa in the absence (□, ○) or presence (■, ●) of a blocking anti-PAI-1 antibody (20 μg/ml) for 2 hrs. at 37°C. FXIa activity was determined by adding S-2366. ( B ) HUVECs were incubated for 16 hrs with TNFα (0.5 ng/ml). Subsequently, cells were incubated with FXIa (500 pM) in the absence (black bars) or presence (white bars) of an anti-PAI-1 antibody (20 μg/ml) for 2 hrs at 37°C. FXIa activity was determined by adding S-2366. ( C ) Empty wells ( ○ ) or HUVECs (□, ●) were incubated with FXIa in the absence (□) or presence (●) of t-PA (30 nM) for 2 hrs at 37°C. FXIa activity was determined by adding S-2366 ( D ) Increasing concentrations of rPAI-1 were incubated with 5 nM FXIa, kallikrein or t-PA at 37°C for 30 min and activity was determined by adding S-2366, S2302 or S2288 respectively. ( E ) PAI-1 detection by western blot in ECs or platelets supernatant. ( F ) FXIa (150 pM) were incubated with vehicle or supernatant from activated platelets in the presence (white bars) or absence (black bars) of an anti-PAI-1 antibody. ( G ) HUVECs were incubated with 0.1 nM FXIa in the presence or absence of a blocking anti-PAI-1 antibody (20 μg/ml) for 2 hrs at 37°C. Fibrin generation was determined in recalcified PPP in the presence of CTI and a blocking anti-TF antibody. ( H ) HUVECs were incubated with TNFα. Fibrin generation was determine in PPP in the presence of CTI or FXI depleted plasma (FXI-dep) in the presence or absence of an anti-TF, an anti-FXI or an anti-PAI-1 antibody. Data are mean ± SD (n = 3). *P

    Journal: Arteriosclerosis, thrombosis, and vascular biology

    Article Title: Endothelial plasminogen activator inhibitor-1 blocks the intrinsic pathway of coagulation, inducing the clearance and degradation of factor XIa.

    doi: 10.1161/ATVBAHA.119.312619

    Figure Lengend Snippet: Detection of FXIa activity in the presence of a blocking anti-PAI-1 antibody. ( A ) Empty wells (●, ○) or HUVECs (■, □) were incubated with FXIa in the absence (□, ○) or presence (■, ●) of a blocking anti-PAI-1 antibody (20 μg/ml) for 2 hrs. at 37°C. FXIa activity was determined by adding S-2366. ( B ) HUVECs were incubated for 16 hrs with TNFα (0.5 ng/ml). Subsequently, cells were incubated with FXIa (500 pM) in the absence (black bars) or presence (white bars) of an anti-PAI-1 antibody (20 μg/ml) for 2 hrs at 37°C. FXIa activity was determined by adding S-2366. ( C ) Empty wells ( ○ ) or HUVECs (□, ●) were incubated with FXIa in the absence (□) or presence (●) of t-PA (30 nM) for 2 hrs at 37°C. FXIa activity was determined by adding S-2366 ( D ) Increasing concentrations of rPAI-1 were incubated with 5 nM FXIa, kallikrein or t-PA at 37°C for 30 min and activity was determined by adding S-2366, S2302 or S2288 respectively. ( E ) PAI-1 detection by western blot in ECs or platelets supernatant. ( F ) FXIa (150 pM) were incubated with vehicle or supernatant from activated platelets in the presence (white bars) or absence (black bars) of an anti-PAI-1 antibody. ( G ) HUVECs were incubated with 0.1 nM FXIa in the presence or absence of a blocking anti-PAI-1 antibody (20 μg/ml) for 2 hrs at 37°C. Fibrin generation was determined in recalcified PPP in the presence of CTI and a blocking anti-TF antibody. ( H ) HUVECs were incubated with TNFα. Fibrin generation was determine in PPP in the presence of CTI or FXI depleted plasma (FXI-dep) in the presence or absence of an anti-TF, an anti-FXI or an anti-PAI-1 antibody. Data are mean ± SD (n = 3). *P

    Article Snippet: Plasma-derived FXIIa, PK, kallikrein, HK and corn trypsin inhibitor (CTI) were from Enzyme Research Laboratories, Inc. (South Bend, IN, USA).

    Techniques: Activity Assay, Blocking Assay, Incubation, Western Blot