truseq small rna library kit Illumina Inc Search Results


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  • 87
    Illumina Inc barcoded srna seq libraries
    Barcoded Srna Seq Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Illumina Inc truseq small rna library preparation kit
    Effect of adapter modifications on tagged library population. Libraries were prepped with 1000 ng human brain total <t>RNA</t> and the CleanTag library prep protocol or recommended manufacturers conditions for Illumina and NEB kits. Data analysis performed by TSRI. A) Correlation plot of unmodified adapters and modified CleanTag adapters within the CleanTag library prep. Tagged miRNA are plotted after Log2 transformation. B) Venn diagram of CleanTag kit, Illumina <t>TruSeq</t> kit, and NEBNext kit depicting number of brain miRNA identified in all 3 replicates for each workflow.
    Truseq Small Rna Library Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 694 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq small rna library preparation kit/product/Illumina Inc
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    99
    Illumina Inc truseq srna kit
    Effect of adapter modifications on tagged library population. Libraries were prepped with 1000 ng human brain total <t>RNA</t> and the CleanTag library prep protocol or recommended manufacturers conditions for Illumina and NEB kits. Data analysis performed by TSRI. A) Correlation plot of unmodified adapters and modified CleanTag adapters within the CleanTag library prep. Tagged miRNA are plotted after Log2 transformation. B) Venn diagram of CleanTag kit, Illumina <t>TruSeq</t> kit, and NEBNext kit depicting number of brain miRNA identified in all 3 replicates for each workflow.
    Truseq Srna Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc truseq small rna library prep
    Effect of adapter modifications on tagged library population. Libraries were prepped with 1000 ng human brain total <t>RNA</t> and the CleanTag library prep protocol or recommended manufacturers conditions for Illumina and NEB kits. Data analysis performed by TSRI. A) Correlation plot of unmodified adapters and modified CleanTag adapters within the CleanTag library prep. Tagged miRNA are plotted after Log2 transformation. B) Venn diagram of CleanTag kit, Illumina <t>TruSeq</t> kit, and NEBNext kit depicting number of brain miRNA identified in all 3 replicates for each workflow.
    Truseq Small Rna Library Prep, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq small rna library prep/product/Illumina Inc
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    Illumina Inc truseq small rna library preparation kits
    Effect of adapter modifications on tagged library population. Libraries were prepped with 1000 ng human brain total <t>RNA</t> and the CleanTag library prep protocol or recommended manufacturers conditions for Illumina and NEB kits. Data analysis performed by TSRI. A) Correlation plot of unmodified adapters and modified CleanTag adapters within the CleanTag library prep. Tagged miRNA are plotted after Log2 transformation. B) Venn diagram of CleanTag kit, Illumina <t>TruSeq</t> kit, and NEBNext kit depicting number of brain miRNA identified in all 3 replicates for each workflow.
    Truseq Small Rna Library Preparation Kits, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq small rna library preparation kits/product/Illumina Inc
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    75
    Illumina Inc truseq small rna library prep kits v2
    Effect of adapter modifications on tagged library population. Libraries were prepped with 1000 ng human brain total <t>RNA</t> and the CleanTag library prep protocol or recommended manufacturers conditions for Illumina and NEB kits. Data analysis performed by TSRI. A) Correlation plot of unmodified adapters and modified CleanTag adapters within the CleanTag library prep. Tagged miRNA are plotted after Log2 transformation. B) Venn diagram of CleanTag kit, Illumina <t>TruSeq</t> kit, and NEBNext kit depicting number of brain miRNA identified in all 3 replicates for each workflow.
    Truseq Small Rna Library Prep Kits V2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Illumina Inc truseq rna sample prep kit
    Effect of adapter modifications on tagged library population. Libraries were prepped with 1000 ng human brain total <t>RNA</t> and the CleanTag library prep protocol or recommended manufacturers conditions for Illumina and NEB kits. Data analysis performed by TSRI. A) Correlation plot of unmodified adapters and modified CleanTag adapters within the CleanTag library prep. Tagged miRNA are plotted after Log2 transformation. B) Venn diagram of CleanTag kit, Illumina <t>TruSeq</t> kit, and NEBNext kit depicting number of brain miRNA identified in all 3 replicates for each workflow.
    Truseq Rna Sample Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 3625 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 3625 article reviews
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    90
    Illumina Inc truseq small rna library preparation kit protocol
    Effect of adapter modifications on tagged library population. Libraries were prepped with 1000 ng human brain total <t>RNA</t> and the CleanTag library prep protocol or recommended manufacturers conditions for Illumina and NEB kits. Data analysis performed by TSRI. A) Correlation plot of unmodified adapters and modified CleanTag adapters within the CleanTag library prep. Tagged miRNA are plotted after Log2 transformation. B) Venn diagram of CleanTag kit, Illumina <t>TruSeq</t> kit, and NEBNext kit depicting number of brain miRNA identified in all 3 replicates for each workflow.
    Truseq Small Rna Library Preparation Kit Protocol, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq small rna library preparation kit protocol/product/Illumina Inc
    Average 90 stars, based on 17 article reviews
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    90
    Illumina Inc truseq small rna sample kit
    a Average percentage of reads mapped to the human transcriptome to <t>RNA</t> biotypes for Illumina <t>TruSeq</t> and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex
    Truseq Small Rna Sample Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq small rna sample kit/product/Illumina Inc
    Average 90 stars, based on 156 article reviews
    Price from $9.99 to $1999.99
    truseq small rna sample kit - by Bioz Stars, 2020-02
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    82
    Illumina Inc truseq small rna adapters
    a Average percentage of reads mapped to the human transcriptome to <t>RNA</t> biotypes for Illumina <t>TruSeq</t> and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex
    Truseq Small Rna Adapters, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 82/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc truseq small rna
    a Average percentage of reads mapped to the human transcriptome to <t>RNA</t> biotypes for Illumina <t>TruSeq</t> and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex
    Truseq Small Rna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq small rna/product/Illumina Inc
    Average 99 stars, based on 41 article reviews
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    truseq small rna - by Bioz Stars, 2020-02
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    90
    Illumina Inc truseq small rna sequencing kit
    a Average percentage of reads mapped to the human transcriptome to <t>RNA</t> biotypes for Illumina <t>TruSeq</t> and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex
    Truseq Small Rna Sequencing Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq small rna sequencing kit/product/Illumina Inc
    Average 90 stars, based on 73 article reviews
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    Illumina Inc truseq stranded small rna library prep kit
    a Average percentage of reads mapped to the human transcriptome to <t>RNA</t> biotypes for Illumina <t>TruSeq</t> and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex
    Truseq Stranded Small Rna Library Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq stranded small rna library prep kit/product/Illumina Inc
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    77
    Illumina Inc truseq small rna 3
    a Average percentage of reads mapped to the human transcriptome to <t>RNA</t> biotypes for Illumina <t>TruSeq</t> and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex
    Truseq Small Rna 3, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc truseq small rna protocol
    a Average percentage of reads mapped to the human transcriptome to <t>RNA</t> biotypes for Illumina <t>TruSeq</t> and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex
    Truseq Small Rna Protocol, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq small rna protocol/product/Illumina Inc
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    90
    Illumina Inc truseq small rna library preparation kit v2
    ( a ) Relative abundances of different small <t>RNA</t> species and tRNA-half types in libraries prepared using the NEBNext Multiplex Small RNA library prep kit, the <t>TruSeq</t> Small RNA library prep kit and the TailorMix miRNA sample preparation kit v2. Depicted values represent the amount of small RNA reads as a percentage of the mapped reads. Values represent the mean of two replicates. ( b ) Length distribution of the mapped reads detected in the NEBNext (red), TailorMix (grey) and TruSeq(blue) libraries.
    Truseq Small Rna Library Preparation Kit V2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq small rna library preparation kit v2/product/Illumina Inc
    Average 90 stars, based on 14 article reviews
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    truseq small rna library preparation kit v2 - by Bioz Stars, 2020-02
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    90
    Illumina Inc truseq rna sample preparation kit
    ( a ) Relative abundances of different small <t>RNA</t> species and tRNA-half types in libraries prepared using the NEBNext Multiplex Small RNA library prep kit, the <t>TruSeq</t> Small RNA library prep kit and the TailorMix miRNA sample preparation kit v2. Depicted values represent the amount of small RNA reads as a percentage of the mapped reads. Values represent the mean of two replicates. ( b ) Length distribution of the mapped reads detected in the NEBNext (red), TailorMix (grey) and TruSeq(blue) libraries.
    Truseq Rna Sample Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 6339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq rna sample preparation kit/product/Illumina Inc
    Average 90 stars, based on 6339 article reviews
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    90
    Illumina Inc truseq stranded mrna
    ( a ) Relative abundances of different small <t>RNA</t> species and tRNA-half types in libraries prepared using the NEBNext Multiplex Small RNA library prep kit, the <t>TruSeq</t> Small RNA library prep kit and the TailorMix miRNA sample preparation kit v2. Depicted values represent the amount of small RNA reads as a percentage of the mapped reads. Values represent the mean of two replicates. ( b ) Length distribution of the mapped reads detected in the NEBNext (red), TailorMix (grey) and TruSeq(blue) libraries.
    Truseq Stranded Mrna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1864 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc cdna library construction
    ( a ) Relative abundances of different small <t>RNA</t> species and tRNA-half types in libraries prepared using the NEBNext Multiplex Small RNA library prep kit, the <t>TruSeq</t> Small RNA library prep kit and the TailorMix miRNA sample preparation kit v2. Depicted values represent the amount of small RNA reads as a percentage of the mapped reads. Values represent the mean of two replicates. ( b ) Length distribution of the mapped reads detected in the NEBNext (red), TailorMix (grey) and TruSeq(blue) libraries.
    Cdna Library Construction, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 3746 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Illumina Inc truseq targeted rna expression kit
    ( a ) Relative abundances of different small <t>RNA</t> species and tRNA-half types in libraries prepared using the NEBNext Multiplex Small RNA library prep kit, the <t>TruSeq</t> Small RNA library prep kit and the TailorMix miRNA sample preparation kit v2. Depicted values represent the amount of small RNA reads as a percentage of the mapped reads. Values represent the mean of two replicates. ( b ) Length distribution of the mapped reads detected in the NEBNext (red), TailorMix (grey) and TruSeq(blue) libraries.
    Truseq Targeted Rna Expression Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq targeted rna expression kit/product/Illumina Inc
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    99
    Illumina Inc truseq stranded mrna sample prep kit
    ( a ) Relative abundances of different small <t>RNA</t> species and tRNA-half types in libraries prepared using the NEBNext Multiplex Small RNA library prep kit, the <t>TruSeq</t> Small RNA library prep kit and the TailorMix miRNA sample preparation kit v2. Depicted values represent the amount of small RNA reads as a percentage of the mapped reads. Values represent the mean of two replicates. ( b ) Length distribution of the mapped reads detected in the NEBNext (red), TailorMix (grey) and TruSeq(blue) libraries.
    Truseq Stranded Mrna Sample Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1652 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq stranded mrna sample prep kit/product/Illumina Inc
    Average 99 stars, based on 1652 article reviews
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    94
    Illumina Inc truseq mirna sample prep kit v2
    ( a ) Relative abundances of different small <t>RNA</t> species and tRNA-half types in libraries prepared using the NEBNext Multiplex Small RNA library prep kit, the <t>TruSeq</t> Small RNA library prep kit and the TailorMix miRNA sample preparation kit v2. Depicted values represent the amount of small RNA reads as a percentage of the mapped reads. Values represent the mean of two replicates. ( b ) Length distribution of the mapped reads detected in the NEBNext (red), TailorMix (grey) and TruSeq(blue) libraries.
    Truseq Mirna Sample Prep Kit V2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq mirna sample prep kit v2/product/Illumina Inc
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    Image Search Results


    Effect of adapter modifications on tagged library population. Libraries were prepped with 1000 ng human brain total RNA and the CleanTag library prep protocol or recommended manufacturers conditions for Illumina and NEB kits. Data analysis performed by TSRI. A) Correlation plot of unmodified adapters and modified CleanTag adapters within the CleanTag library prep. Tagged miRNA are plotted after Log2 transformation. B) Venn diagram of CleanTag kit, Illumina TruSeq kit, and NEBNext kit depicting number of brain miRNA identified in all 3 replicates for each workflow.

    Journal: PLoS ONE

    Article Title: Small RNA Library Preparation Method for Next-Generation Sequencing Using Chemical Modifications to Prevent Adapter Dimer Formation

    doi: 10.1371/journal.pone.0167009

    Figure Lengend Snippet: Effect of adapter modifications on tagged library population. Libraries were prepped with 1000 ng human brain total RNA and the CleanTag library prep protocol or recommended manufacturers conditions for Illumina and NEB kits. Data analysis performed by TSRI. A) Correlation plot of unmodified adapters and modified CleanTag adapters within the CleanTag library prep. Tagged miRNA are plotted after Log2 transformation. B) Venn diagram of CleanTag kit, Illumina TruSeq kit, and NEBNext kit depicting number of brain miRNA identified in all 3 replicates for each workflow.

    Article Snippet: The two most common commercially available kits, TruSeq Small RNA Library Preparation Kit (Illumina) and NEBNext Small RNA Library Prep Set (New England Biolabs), recommend 100 ng total RNA input as the lowest sample amount achievable [ ].

    Techniques: Modification, Transformation Assay

    NGS data comparison between CleanTag and TruSeq Small RNA Library Preparation Kit. Libraries prepared with TruSeq Small RNA Library Preparation Kit or CleanTag workflow with total human brain RNA input and gel purification. Samples sequenced on a HiSeq 2500 SR, 1x 100bp. Human total brain RNA at A) 100 ng, or B) 10 ng input. Data analysis performed using Geneious. Statistical analysis performed with GraphPad-One way ANOVA Turkeys multiple comparison test.

    Journal: PLoS ONE

    Article Title: Small RNA Library Preparation Method for Next-Generation Sequencing Using Chemical Modifications to Prevent Adapter Dimer Formation

    doi: 10.1371/journal.pone.0167009

    Figure Lengend Snippet: NGS data comparison between CleanTag and TruSeq Small RNA Library Preparation Kit. Libraries prepared with TruSeq Small RNA Library Preparation Kit or CleanTag workflow with total human brain RNA input and gel purification. Samples sequenced on a HiSeq 2500 SR, 1x 100bp. Human total brain RNA at A) 100 ng, or B) 10 ng input. Data analysis performed using Geneious. Statistical analysis performed with GraphPad-One way ANOVA Turkeys multiple comparison test.

    Article Snippet: The two most common commercially available kits, TruSeq Small RNA Library Preparation Kit (Illumina) and NEBNext Small RNA Library Prep Set (New England Biolabs), recommend 100 ng total RNA input as the lowest sample amount achievable [ ].

    Techniques: Next-Generation Sequencing, Gel Purification

    Comparison of crude and bead purified libraries using CleanTag or TruSeq small RNA library prep kit. Bioanalyzer traces of libraries prepared using 1000 ng human total brain RNA input. Crude or AMPure XP purified PCR products with A) TruSeq small RNA library preparation kit, or B) CleanTag small RNA library preparation kit.

    Journal: PLoS ONE

    Article Title: Small RNA Library Preparation Method for Next-Generation Sequencing Using Chemical Modifications to Prevent Adapter Dimer Formation

    doi: 10.1371/journal.pone.0167009

    Figure Lengend Snippet: Comparison of crude and bead purified libraries using CleanTag or TruSeq small RNA library prep kit. Bioanalyzer traces of libraries prepared using 1000 ng human total brain RNA input. Crude or AMPure XP purified PCR products with A) TruSeq small RNA library preparation kit, or B) CleanTag small RNA library preparation kit.

    Article Snippet: The two most common commercially available kits, TruSeq Small RNA Library Preparation Kit (Illumina) and NEBNext Small RNA Library Prep Set (New England Biolabs), recommend 100 ng total RNA input as the lowest sample amount achievable [ ].

    Techniques: Purification, Polymerase Chain Reaction

    a Average percentage of reads mapped to the human transcriptome to RNA biotypes for Illumina TruSeq and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex

    Journal: BMC Genomics

    Article Title: Evaluation of commercially available small RNASeq library preparation kits using low input RNA

    doi: 10.1186/s12864-018-4726-6

    Figure Lengend Snippet: a Average percentage of reads mapped to the human transcriptome to RNA biotypes for Illumina TruSeq and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex

    Article Snippet: Illumina TruSeq small RNA library preparation Small RNA libraries were generated using Illumina TruSeq Small RNA Sample kit (RS-200-0048; Illumina).

    Techniques: Expressing

    Schematic of study design. Tissue RNA from brain, liver and placenta were sequenced at two sites at two input amounts (1 μg and 10 ng) using three different RNA sequencing kits (Illumina TruSeq, NEB Next and BiooScientific NEXTFlex). RNA from plasma samples at 5 different input volumes (200 μL – 5 mL) were sequenced at Site 1 using only TruSeq and BiooScientific. The green arrow depicts the flow of one of the tissue samples – brain using NEB Next and the red arrows, the plasma samples. The RNASeq results from the tissue samples were then validated using three different platforms (qPCR, EdgeSeq performed by Site1 and Fireplex performed by Site2). For a full list of samples sequenced, please refer to Additional file 1 : Table S1

    Journal: BMC Genomics

    Article Title: Evaluation of commercially available small RNASeq library preparation kits using low input RNA

    doi: 10.1186/s12864-018-4726-6

    Figure Lengend Snippet: Schematic of study design. Tissue RNA from brain, liver and placenta were sequenced at two sites at two input amounts (1 μg and 10 ng) using three different RNA sequencing kits (Illumina TruSeq, NEB Next and BiooScientific NEXTFlex). RNA from plasma samples at 5 different input volumes (200 μL – 5 mL) were sequenced at Site 1 using only TruSeq and BiooScientific. The green arrow depicts the flow of one of the tissue samples – brain using NEB Next and the red arrows, the plasma samples. The RNASeq results from the tissue samples were then validated using three different platforms (qPCR, EdgeSeq performed by Site1 and Fireplex performed by Site2). For a full list of samples sequenced, please refer to Additional file 1 : Table S1

    Article Snippet: Illumina TruSeq small RNA library preparation Small RNA libraries were generated using Illumina TruSeq Small RNA Sample kit (RS-200-0048; Illumina).

    Techniques: RNA Sequencing Assay, Flow Cytometry, Real-time Polymerase Chain Reaction

    ( a ) Relative abundances of different small RNA species and tRNA-half types in libraries prepared using the NEBNext Multiplex Small RNA library prep kit, the TruSeq Small RNA library prep kit and the TailorMix miRNA sample preparation kit v2. Depicted values represent the amount of small RNA reads as a percentage of the mapped reads. Values represent the mean of two replicates. ( b ) Length distribution of the mapped reads detected in the NEBNext (red), TailorMix (grey) and TruSeq(blue) libraries.

    Journal: Scientific Reports

    Article Title: Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples

    doi: 10.1038/srep37876

    Figure Lengend Snippet: ( a ) Relative abundances of different small RNA species and tRNA-half types in libraries prepared using the NEBNext Multiplex Small RNA library prep kit, the TruSeq Small RNA library prep kit and the TailorMix miRNA sample preparation kit v2. Depicted values represent the amount of small RNA reads as a percentage of the mapped reads. Values represent the mean of two replicates. ( b ) Length distribution of the mapped reads detected in the NEBNext (red), TailorMix (grey) and TruSeq(blue) libraries.

    Article Snippet: Small RNA sequencing For Small RNA library preparation we used the TailorMix miRNA sample preparation kit v2 (Seqmatic), the NEBNext Multiplex Small RNA library prep kit (New England Biolabs) or the TruSeq small RNA library preparation kit v2 (Illumina) following manufacturer’s instructions with small modifications ( ).

    Techniques: Multiplex Assay, Sample Prep