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  • 99
    Thermo Fisher trizol reagent thermo fisher
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    Trizol Reagent Thermo Fisher, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29047 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher a1203 trizol reagent thermo fisher scientific
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    A1203 Trizol Reagent Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher s7023 trizol reagent thermo fisher scientific
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    S7023 Trizol Reagent Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher s11494 trizol reagent thermo fisher scientific
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    S11494 Trizol Reagent Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 2014 n a trizol reagent thermo fisher scientific
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    2014 N A Trizol Reagent Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher x 100 trizol reagent thermo fisher scientific
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    X 100 Trizol Reagent Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trizolⓡ reagent
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    Trizolⓡ Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trizol¯ reagent
    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), <t>RNeasy</t> Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and <t>TRIzol</t> Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
    Trizol¯ Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher trizolls reagent
    Viral protein expression and dsRNA production in siRNA-transfected cells. CV-1 cells were transfected by electroporation with either no siRNA, control siRNA, M3-si01, M3-si02, or M3-si03 as indicated in each panel. The cells were infected with MRV strain T1L, T2J, or T3D at 24 h p.t. at an MOI of 5. (a–c) Cells were harvested at 24 h p.i. by washing with PBS and then lysing in 2× sample buffer. Lysates were boiled for 5 min, separated by SDS/PAGE, transferred to nitrocellulose, and immunoblotted with polyclonal μNS antiserum (a) or polyclonal T1L virion antiserum (b). GAPDH immunoblot (c) was included as a loading control. (d) Cells were harvested at 24 h p.i. by washing with PBS and then purifying total RNA by <t>TrizolLS</t> extraction. Samples were heated to 60°C for 5 min, and dsRNAs (large, L; medium, M; and small, S as indicated) were resolved by SDS/PAGE. The gel was stained with 0.5% ethidium bromide.
    Trizolls Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 286 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trizole reagent
    Proinflammatory cytokine mRNA expression in LPS-primed U937 monocytic cells. U937 cells were pulsed with graded concentrations of LPS (see Materials and Methods). The cells were harvested after 8 h of incubation and lysed. Total cellular RNA was isolated with <t>Trizole</t> reagent (see Materials and Methods) and subjected to RT with AMV reverse transcriptase. The cDNA thus obtained was amplified by PCR with specific primers for IL-1β, IL-6, and TNF-α. β-Actin was used as an internal control. The levels of expression of various cytokines were expressed as ratios of cytokines to β-actin. In U937 monocytic cells, LPS enhances the expression of TNF-α, IL-1β, and IL-6 in a concentration-dependent manner.
    Trizole Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher reagents trizol kit
    Proinflammatory cytokine mRNA expression in LPS-primed U937 monocytic cells. U937 cells were pulsed with graded concentrations of LPS (see Materials and Methods). The cells were harvested after 8 h of incubation and lysed. Total cellular RNA was isolated with <t>Trizole</t> reagent (see Materials and Methods) and subjected to RT with AMV reverse transcriptase. The cDNA thus obtained was amplified by PCR with specific primers for IL-1β, IL-6, and TNF-α. β-Actin was used as an internal control. The levels of expression of various cytokines were expressed as ratios of cytokines to β-actin. In U937 monocytic cells, LPS enhances the expression of TNF-α, IL-1β, and IL-6 in a concentration-dependent manner.
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    TaKaRa trizol reagent
    Proinflammatory cytokine mRNA expression in LPS-primed U937 monocytic cells. U937 cells were pulsed with graded concentrations of LPS (see Materials and Methods). The cells were harvested after 8 h of incubation and lysed. Total cellular RNA was isolated with <t>Trizole</t> reagent (see Materials and Methods) and subjected to RT with AMV reverse transcriptase. The cDNA thus obtained was amplified by PCR with specific primers for IL-1β, IL-6, and TNF-α. β-Actin was used as an internal control. The levels of expression of various cytokines were expressed as ratios of cytokines to β-actin. In U937 monocytic cells, LPS enhances the expression of TNF-α, IL-1β, and IL-6 in a concentration-dependent manner.
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    Thermo Fisher trizol℘ reagent
    Proinflammatory cytokine mRNA expression in LPS-primed U937 monocytic cells. U937 cells were pulsed with graded concentrations of LPS (see Materials and Methods). The cells were harvested after 8 h of incubation and lysed. Total cellular RNA was isolated with <t>Trizole</t> reagent (see Materials and Methods) and subjected to RT with AMV reverse transcriptase. The cDNA thus obtained was amplified by PCR with specific primers for IL-1β, IL-6, and TNF-α. β-Actin was used as an internal control. The levels of expression of various cytokines were expressed as ratios of cytokines to β-actin. In U937 monocytic cells, LPS enhances the expression of TNF-α, IL-1β, and IL-6 in a concentration-dependent manner.
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    Thermo Fisher o reagente trizol
    Proinflammatory cytokine mRNA expression in LPS-primed U937 monocytic cells. U937 cells were pulsed with graded concentrations of LPS (see Materials and Methods). The cells were harvested after 8 h of incubation and lysed. Total cellular RNA was isolated with <t>Trizole</t> reagent (see Materials and Methods) and subjected to RT with AMV reverse transcriptase. The cDNA thus obtained was amplified by PCR with specific primers for IL-1β, IL-6, and TNF-α. β-Actin was used as an internal control. The levels of expression of various cytokines were expressed as ratios of cytokines to β-actin. In U937 monocytic cells, LPS enhances the expression of TNF-α, IL-1β, and IL-6 in a concentration-dependent manner.
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    Image Search Results


    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), RNeasy Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and TRIzol Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip

    Journal: Virology Journal

    Article Title: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics

    doi: 10.1186/s12985-015-0376-3

    Figure Lengend Snippet: Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), RNeasy Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and TRIzol Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip

    Article Snippet: Commercial RNA isolation kits Five commercial kits were selected for this study, which included TRIzol Reagent (Life Technologies), RNeasy Plant mini kit (Qiagen), Spectrum™ Plant Total RNA kit (Sigma), AccuPrep viral RNA extraction kit (Bioneer) and Plant/fungi total RNA kit (Norgen BioTek).

    Techniques: Isolation, Nucleic Acid Electrophoresis, RNA Extraction, Electrophoresis, Chromatin Immunoprecipitation

    THSG inhibited vascular-remodelling-related gene expression. Total RNA was extracted from rat aortas by using Invitrogen TRIzol reagent. ((a) to (l)) COL1A2, ACTCA, TIMP1, CCL3, IGFBP1, ECE1, KLF5, MYL1, WISP2, COL3A1, BMP4, and FN1 mRNA expression was measured using a Maxima SYBR Green qPCR Master Mix kit. Data are normalised to GAPDH mRNA expression levels ( n = 4). Data are expressed as the mean ± SEM. ∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Aortic Remodelling Is Improved by 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside Involving the Smad3 Pathway in Spontaneously Hypertensive Rats

    doi: 10.1155/2015/789027

    Figure Lengend Snippet: THSG inhibited vascular-remodelling-related gene expression. Total RNA was extracted from rat aortas by using Invitrogen TRIzol reagent. ((a) to (l)) COL1A2, ACTCA, TIMP1, CCL3, IGFBP1, ECE1, KLF5, MYL1, WISP2, COL3A1, BMP4, and FN1 mRNA expression was measured using a Maxima SYBR Green qPCR Master Mix kit. Data are normalised to GAPDH mRNA expression levels ( n = 4). Data are expressed as the mean ± SEM. ∗ P

    Article Snippet: RNA Isolation and Quantitative PCR Total RNA was extracted from the rat aortas with the Invitrogen TRIzol reagent (Life Technologies, Grand Island, USA) according to the manufacturer's instructions.

    Techniques: Expressing, SYBR Green Assay, Real-time Polymerase Chain Reaction

    Viral protein expression and dsRNA production in siRNA-transfected cells. CV-1 cells were transfected by electroporation with either no siRNA, control siRNA, M3-si01, M3-si02, or M3-si03 as indicated in each panel. The cells were infected with MRV strain T1L, T2J, or T3D at 24 h p.t. at an MOI of 5. (a–c) Cells were harvested at 24 h p.i. by washing with PBS and then lysing in 2× sample buffer. Lysates were boiled for 5 min, separated by SDS/PAGE, transferred to nitrocellulose, and immunoblotted with polyclonal μNS antiserum (a) or polyclonal T1L virion antiserum (b). GAPDH immunoblot (c) was included as a loading control. (d) Cells were harvested at 24 h p.i. by washing with PBS and then purifying total RNA by TrizolLS extraction. Samples were heated to 60°C for 5 min, and dsRNAs (large, L; medium, M; and small, S as indicated) were resolved by SDS/PAGE. The gel was stained with 0.5% ethidium bromide.

    Journal: Virology

    Article Title: Formation of the factory matrix is an important, though not a sufficient function of nonstructural protein ?NS during reovirus infection

    doi: 10.1016/j.virol.2008.02.024

    Figure Lengend Snippet: Viral protein expression and dsRNA production in siRNA-transfected cells. CV-1 cells were transfected by electroporation with either no siRNA, control siRNA, M3-si01, M3-si02, or M3-si03 as indicated in each panel. The cells were infected with MRV strain T1L, T2J, or T3D at 24 h p.t. at an MOI of 5. (a–c) Cells were harvested at 24 h p.i. by washing with PBS and then lysing in 2× sample buffer. Lysates were boiled for 5 min, separated by SDS/PAGE, transferred to nitrocellulose, and immunoblotted with polyclonal μNS antiserum (a) or polyclonal T1L virion antiserum (b). GAPDH immunoblot (c) was included as a loading control. (d) Cells were harvested at 24 h p.i. by washing with PBS and then purifying total RNA by TrizolLS extraction. Samples were heated to 60°C for 5 min, and dsRNAs (large, L; medium, M; and small, S as indicated) were resolved by SDS/PAGE. The gel was stained with 0.5% ethidium bromide.

    Article Snippet: Cell lysates were collected at specified times p.i. using TrizolLS reagent (Invitrogen) according to the manufacturer’s instructions.

    Techniques: Expressing, Transfection, Electroporation, Infection, SDS Page, Staining

    Characterization of HBV DNA and RNA in sera of CHB patients. (A and B) Analyses of serum viral DNA from CHB patients by Southern blotting. Viral DNA was extracted from serum samples obtained from forty-five chronic hepatitis B patients (20% of input sample used for protein A/G agarose beads pulldown) and subjected to Southern blot analysis. Alternatively, these samples were first incubated with protein A/G agarose beads, and then viral DNA in the pulldown mixtures was analyzed by Southern blotting. Serum samples selected for further examining are marked with arrows, and samples with SS DNA detection are labeled with asterisks. (C) Protein A/G agarose bead pulldown of viral particles. Sera (25 μl each) from CHB patients 37, 38, 14, and 35 (M1, mixture one) or from patients 17, 21, 42, and 44 (M2, mixture two) were pooled and incubated with protein A/G agarose beads. Viral DNA in input sera, protein A/G bead pulldown mixtures (beads), and the remaining supernatants (sup.) were extracted and subjected to Southern blot analysis. (D) Northern blot detection of serum viral RNA from patients 37, 38, 14, 35, 17, 21, 42, and 44. Total RNA were extracted from serum samples by TRIzol reagent and treated with DNase I before Northern blot analysis. (E to G) Southern blot analyses of viral DNA from selected samples. Viral DNA was separated by electrophoresis through TAE or alkaline agarose gels, followed by Southern blot detection with the indicated riboprobes.

    Journal: Journal of Virology

    Article Title: Extracellular Hepatitis B Virus RNAs Are Heterogeneous in Length and Circulate as Capsid-Antibody Complexes in Addition to Virions in Chronic Hepatitis B Patients

    doi: 10.1128/JVI.00798-18

    Figure Lengend Snippet: Characterization of HBV DNA and RNA in sera of CHB patients. (A and B) Analyses of serum viral DNA from CHB patients by Southern blotting. Viral DNA was extracted from serum samples obtained from forty-five chronic hepatitis B patients (20% of input sample used for protein A/G agarose beads pulldown) and subjected to Southern blot analysis. Alternatively, these samples were first incubated with protein A/G agarose beads, and then viral DNA in the pulldown mixtures was analyzed by Southern blotting. Serum samples selected for further examining are marked with arrows, and samples with SS DNA detection are labeled with asterisks. (C) Protein A/G agarose bead pulldown of viral particles. Sera (25 μl each) from CHB patients 37, 38, 14, and 35 (M1, mixture one) or from patients 17, 21, 42, and 44 (M2, mixture two) were pooled and incubated with protein A/G agarose beads. Viral DNA in input sera, protein A/G bead pulldown mixtures (beads), and the remaining supernatants (sup.) were extracted and subjected to Southern blot analysis. (D) Northern blot detection of serum viral RNA from patients 37, 38, 14, 35, 17, 21, 42, and 44. Total RNA were extracted from serum samples by TRIzol reagent and treated with DNase I before Northern blot analysis. (E to G) Southern blot analyses of viral DNA from selected samples. Viral DNA was separated by electrophoresis through TAE or alkaline agarose gels, followed by Southern blot detection with the indicated riboprobes.

    Article Snippet: In addition to the SDS-proteinase K method, viral RNA was also extracted with TRIzol LS reagent according to the manufacturer’s instructions (Thermo Fisher Scientific).

    Techniques: Southern Blot, Incubation, Labeling, Northern Blot, Electrophoresis

    ). (B) Identification of 3′ ends of extracellular HBV RNAs. 3′ Ends of extracellular HBV RNAs were identified by the 3′ RACE method using different HBV-specific anchor primers (the same 5′ primers used for generating templates for producing riboprobes used in panel A, lower). Identified 3′ ends were numbered as described above, and numbers in parentheses indicate the amount of clones with the same 3′ ends. The asterisk indicates unknown nucleic acid copurified with intracellular capsid-associated viral RNA by TRIzol reagent. FL, full-length; Cap, 5′ cap of pregenomic RNA; pA, the polyadenylation site; An, poly(A) tail.

    Journal: Journal of Virology

    Article Title: Extracellular Hepatitis B Virus RNAs Are Heterogeneous in Length and Circulate as Capsid-Antibody Complexes in Addition to Virions in Chronic Hepatitis B Patients

    doi: 10.1128/JVI.00798-18

    Figure Lengend Snippet: ). (B) Identification of 3′ ends of extracellular HBV RNAs. 3′ Ends of extracellular HBV RNAs were identified by the 3′ RACE method using different HBV-specific anchor primers (the same 5′ primers used for generating templates for producing riboprobes used in panel A, lower). Identified 3′ ends were numbered as described above, and numbers in parentheses indicate the amount of clones with the same 3′ ends. The asterisk indicates unknown nucleic acid copurified with intracellular capsid-associated viral RNA by TRIzol reagent. FL, full-length; Cap, 5′ cap of pregenomic RNA; pA, the polyadenylation site; An, poly(A) tail.

    Article Snippet: In addition to the SDS-proteinase K method, viral RNA was also extracted with TRIzol LS reagent according to the manufacturer’s instructions (Thermo Fisher Scientific).

    Techniques: Clone Assay

    Proinflammatory cytokine mRNA expression in LPS-primed U937 monocytic cells. U937 cells were pulsed with graded concentrations of LPS (see Materials and Methods). The cells were harvested after 8 h of incubation and lysed. Total cellular RNA was isolated with Trizole reagent (see Materials and Methods) and subjected to RT with AMV reverse transcriptase. The cDNA thus obtained was amplified by PCR with specific primers for IL-1β, IL-6, and TNF-α. β-Actin was used as an internal control. The levels of expression of various cytokines were expressed as ratios of cytokines to β-actin. In U937 monocytic cells, LPS enhances the expression of TNF-α, IL-1β, and IL-6 in a concentration-dependent manner.

    Journal: Infection and Immunity

    Article Title: Effects of Cytokines and Endotoxin on the Intracellular Growth of Bacteria

    doi:

    Figure Lengend Snippet: Proinflammatory cytokine mRNA expression in LPS-primed U937 monocytic cells. U937 cells were pulsed with graded concentrations of LPS (see Materials and Methods). The cells were harvested after 8 h of incubation and lysed. Total cellular RNA was isolated with Trizole reagent (see Materials and Methods) and subjected to RT with AMV reverse transcriptase. The cDNA thus obtained was amplified by PCR with specific primers for IL-1β, IL-6, and TNF-α. β-Actin was used as an internal control. The levels of expression of various cytokines were expressed as ratios of cytokines to β-actin. In U937 monocytic cells, LPS enhances the expression of TNF-α, IL-1β, and IL-6 in a concentration-dependent manner.

    Article Snippet: Following 8 h of priming with LPS, one batch of U937 cells (2 × 106 cells) from each group mentioned above was harvested, washed, and lysed with Trizole reagent (Life Technologies).

    Techniques: Expressing, Incubation, Isolation, Amplification, Polymerase Chain Reaction, Concentration Assay