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    Thermo Fisher trizolr reagent
    Selective induction of BRLF1 and BZLF1 expression by inducing chemicals in B95-8 and HH514-16 cells. (A) Induction of mRNAs containing BRLF1 and BZLF1 . At 20 h after treatment of the cells with TPA or n -butyrate, singly or in combination, <t>RNA</t> was prepared by the <t>TRIzol</t> method. RNA from 3 × 10 6 cells per lane was analyzed by Northern blotting with a probe prepared from a portion of the BZLF1 cDNA. This probe detects the 4.0- and 3.0-kb bicistronic ( BRLF1 plus BZLF1 ) mRNA from Rp and the 1.0-kb monocistronic ( BZLF1 ). The blot was reprobed with β-actin to control for loading of RNA. (B) Comparison of the effects of n -butyrate (B) and TSA. Cytoplasmic RNA prepared 17 h after chemical treatment was analyzed by Northern blotting with the BZLF1 probe. The RNA blots were also probed for β-actin and the H1 RNA component of human RNase P to control for RNA loading.
    Trizolr Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 60196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 60196 article reviews
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    Selective induction of BRLF1 and BZLF1 expression by inducing chemicals in B95-8 and HH514-16 cells. (A) Induction of mRNAs containing BRLF1 and BZLF1 . At 20 h after treatment of the cells with TPA or n -butyrate, singly or in combination, RNA was prepared by the TRIzol method. RNA from 3 × 10 6 cells per lane was analyzed by Northern blotting with a probe prepared from a portion of the BZLF1 cDNA. This probe detects the 4.0- and 3.0-kb bicistronic ( BRLF1 plus BZLF1 ) mRNA from Rp and the 1.0-kb monocistronic ( BZLF1 ). The blot was reprobed with β-actin to control for loading of RNA. (B) Comparison of the effects of n -butyrate (B) and TSA. Cytoplasmic RNA prepared 17 h after chemical treatment was analyzed by Northern blotting with the BZLF1 probe. The RNA blots were also probed for β-actin and the H1 RNA component of human RNase P to control for RNA loading.

    Journal: Journal of Virology

    Article Title: Protein Kinase C-Independent Activation of the Epstein-Barr Virus Lytic Cycle

    doi: 10.1128/JVI.76.11.5612-5626.2002

    Figure Lengend Snippet: Selective induction of BRLF1 and BZLF1 expression by inducing chemicals in B95-8 and HH514-16 cells. (A) Induction of mRNAs containing BRLF1 and BZLF1 . At 20 h after treatment of the cells with TPA or n -butyrate, singly or in combination, RNA was prepared by the TRIzol method. RNA from 3 × 10 6 cells per lane was analyzed by Northern blotting with a probe prepared from a portion of the BZLF1 cDNA. This probe detects the 4.0- and 3.0-kb bicistronic ( BRLF1 plus BZLF1 ) mRNA from Rp and the 1.0-kb monocistronic ( BZLF1 ). The blot was reprobed with β-actin to control for loading of RNA. (B) Comparison of the effects of n -butyrate (B) and TSA. Cytoplasmic RNA prepared 17 h after chemical treatment was analyzed by Northern blotting with the BZLF1 probe. The RNA blots were also probed for β-actin and the H1 RNA component of human RNase P to control for RNA loading.

    Article Snippet: Total RNA was prepared with RNeasy and Qia-shredder spin columns (Qiagen) or TRIzol reagent (GIBCO/BRL) according to the manufacturers' directions.

    Techniques: Expressing, Northern Blot

    Loss of Utx Affects Gene Expression Profile (A) Cluster analysis of control and UTX conditional knockout (cKO) cortices. The Utx fl/fl (Ctrl) or Utx cKO cortices were isolated at E14 and total RNA was isolated and used for RNA-seq. (B) GO analysis of control and Utx cKO cortices. (C) Volcano plot of control and Utx cKO cortices. Pcna , a marker of proliferation, was upregulated and Pten significantly downregulated in Utx cKO cortices. The genes that were non-significantly regulated are marked with gray points; genes significantly downregulated are marked with green points; genes significantly upregulated are marked with red points. (D) RT-PCR analysis of eNSCs infected with control or Utx shRNA constructs. The cells were collected 3 days after infection and total RNA isolated by TRIzol was used for the RT-PCR. Values are presented as mean ± SEM (n = 3 independent experiments; ∗∗ p

    Journal: Stem Cell Reports

    Article Title: UTX Affects Neural Stem Cell Proliferation and Differentiation through PTEN Signaling

    doi: 10.1016/j.stemcr.2018.02.008

    Figure Lengend Snippet: Loss of Utx Affects Gene Expression Profile (A) Cluster analysis of control and UTX conditional knockout (cKO) cortices. The Utx fl/fl (Ctrl) or Utx cKO cortices were isolated at E14 and total RNA was isolated and used for RNA-seq. (B) GO analysis of control and Utx cKO cortices. (C) Volcano plot of control and Utx cKO cortices. Pcna , a marker of proliferation, was upregulated and Pten significantly downregulated in Utx cKO cortices. The genes that were non-significantly regulated are marked with gray points; genes significantly downregulated are marked with green points; genes significantly upregulated are marked with red points. (D) RT-PCR analysis of eNSCs infected with control or Utx shRNA constructs. The cells were collected 3 days after infection and total RNA isolated by TRIzol was used for the RT-PCR. Values are presented as mean ± SEM (n = 3 independent experiments; ∗∗ p

    Article Snippet: RNA Isolation and qRT-PCR Total RNA was extracted with TRIzol (Invitrogen) and then inverse transcribed into cDNA using the FastQuant RT Kit (Tiangen Biotech).

    Techniques: Expressing, Knock-Out, Isolation, RNA Sequencing Assay, Marker, Reverse Transcription Polymerase Chain Reaction, Infection, shRNA, Construct

    THSG inhibited vascular-remodelling-related gene expression. Total RNA was extracted from rat aortas by using Invitrogen TRIzol reagent. ((a) to (l)) COL1A2, ACTCA, TIMP1, CCL3, IGFBP1, ECE1, KLF5, MYL1, WISP2, COL3A1, BMP4, and FN1 mRNA expression was measured using a Maxima SYBR Green qPCR Master Mix kit. Data are normalised to GAPDH mRNA expression levels ( n = 4). Data are expressed as the mean ± SEM. ∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Aortic Remodelling Is Improved by 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside Involving the Smad3 Pathway in Spontaneously Hypertensive Rats

    doi: 10.1155/2015/789027

    Figure Lengend Snippet: THSG inhibited vascular-remodelling-related gene expression. Total RNA was extracted from rat aortas by using Invitrogen TRIzol reagent. ((a) to (l)) COL1A2, ACTCA, TIMP1, CCL3, IGFBP1, ECE1, KLF5, MYL1, WISP2, COL3A1, BMP4, and FN1 mRNA expression was measured using a Maxima SYBR Green qPCR Master Mix kit. Data are normalised to GAPDH mRNA expression levels ( n = 4). Data are expressed as the mean ± SEM. ∗ P

    Article Snippet: RNA Isolation and Quantitative PCR Total RNA was extracted from the rat aortas with the Invitrogen TRIzol reagent (Life Technologies, Grand Island, USA) according to the manufacturer's instructions.

    Techniques: Expressing, SYBR Green Assay, Real-time Polymerase Chain Reaction