Journal: Evidence-based Complementary and Alternative Medicine : eCAM
Article Title: Aortic Remodelling Is Improved by 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside Involving the Smad3 Pathway in Spontaneously Hypertensive Rats
Figure Lengend Snippet: THSG inhibited vascular-remodelling-related gene expression. Total RNA was extracted from rat aortas by using Invitrogen TRIzol reagent. ((a) to (l)) COL1A2, ACTCA, TIMP1, CCL3, IGFBP1, ECE1, KLF5, MYL1, WISP2, COL3A1, BMP4, and FN1 mRNA expression was measured using a Maxima SYBR Green qPCR Master Mix kit. Data are normalised to GAPDH mRNA expression levels ( n = 4). Data are expressed as the mean ± SEM. ∗ P
Article Snippet: RNA Isolation and Quantitative PCR Total RNA was extracted from the rat aortas with the Invitrogen TRIzol reagent (Life Technologies, Grand Island, USA) according to the manufacturer's instructions.
Techniques: Expressing, SYBR Green Assay, Real-time Polymerase Chain Reaction