trizol Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher trizol trizol reagent
    Loss of Utx Affects Gene Expression Profile (A) Cluster analysis of control and UTX conditional knockout (cKO) cortices. The Utx fl/fl (Ctrl) or Utx cKO cortices were isolated at E14 and total <t>RNA</t> was isolated and used for RNA-seq. (B) GO analysis of control and Utx cKO cortices. (C) Volcano plot of control and Utx cKO cortices. Pcna , a marker of proliferation, was upregulated and Pten significantly downregulated in Utx cKO cortices. The genes that were non-significantly regulated are marked with gray points; genes significantly downregulated are marked with green points; genes significantly upregulated are marked with red points. (D) RT-PCR analysis of eNSCs infected with control or Utx shRNA constructs. The cells were collected 3 days after infection and total RNA isolated by <t>TRIzol</t> was used for the RT-PCR. Values are presented as mean ± SEM (n = 3 independent experiments; ∗∗ p
    Trizol Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 60753 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trizol trizol reagent/product/Thermo Fisher
    Average 99 stars, based on 60753 article reviews
    Price from $9.99 to $1999.99
    trizol trizol reagent - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Loss of Utx Affects Gene Expression Profile (A) Cluster analysis of control and UTX conditional knockout (cKO) cortices. The Utx fl/fl (Ctrl) or Utx cKO cortices were isolated at E14 and total RNA was isolated and used for RNA-seq. (B) GO analysis of control and Utx cKO cortices. (C) Volcano plot of control and Utx cKO cortices. Pcna , a marker of proliferation, was upregulated and Pten significantly downregulated in Utx cKO cortices. The genes that were non-significantly regulated are marked with gray points; genes significantly downregulated are marked with green points; genes significantly upregulated are marked with red points. (D) RT-PCR analysis of eNSCs infected with control or Utx shRNA constructs. The cells were collected 3 days after infection and total RNA isolated by TRIzol was used for the RT-PCR. Values are presented as mean ± SEM (n = 3 independent experiments; ∗∗ p

    Journal: Stem Cell Reports

    Article Title: UTX Affects Neural Stem Cell Proliferation and Differentiation through PTEN Signaling

    doi: 10.1016/j.stemcr.2018.02.008

    Figure Lengend Snippet: Loss of Utx Affects Gene Expression Profile (A) Cluster analysis of control and UTX conditional knockout (cKO) cortices. The Utx fl/fl (Ctrl) or Utx cKO cortices were isolated at E14 and total RNA was isolated and used for RNA-seq. (B) GO analysis of control and Utx cKO cortices. (C) Volcano plot of control and Utx cKO cortices. Pcna , a marker of proliferation, was upregulated and Pten significantly downregulated in Utx cKO cortices. The genes that were non-significantly regulated are marked with gray points; genes significantly downregulated are marked with green points; genes significantly upregulated are marked with red points. (D) RT-PCR analysis of eNSCs infected with control or Utx shRNA constructs. The cells were collected 3 days after infection and total RNA isolated by TRIzol was used for the RT-PCR. Values are presented as mean ± SEM (n = 3 independent experiments; ∗∗ p

    Article Snippet: RNA Isolation and qRT-PCR Total RNA was extracted with TRIzol (Invitrogen) and then inverse transcribed into cDNA using the FastQuant RT Kit (Tiangen Biotech).

    Techniques: Expressing, Knock-Out, Isolation, RNA Sequencing Assay, Marker, Reverse Transcription Polymerase Chain Reaction, Infection, shRNA, Construct

    Cytokine gene expression is reduced during CHIKV Opal524R infection. (A, B) Twenty-four-day-old C57BL6/J mice were infected in the left rear footpad with 100 PFU of wild-type CHIKV ( n = 5) or Opal524R ( n = 5) or mock infected with diluent ( n = 3). At days 1 (A) and 2 (B) postinfection, mice were sacrificed and perfused intracardially with 1× PBS, and the ipsilateral foot was harvested in TRIzol. RNA was extracted, cDNA was generated, and cytokine gene expression was assessed by quantitative real-time PCR. Each cytokine was normalized to host 18S gene expression. Fold changes in gene expression relative to mock infection (left) and wild-type infection (right) are shown. Data are representative of two independent experiments. Statistical significance is indicated as follows: *, P

    Journal: mBio

    Article Title: Disruption of the Opal Stop Codon Attenuates Chikungunya Virus-Induced Arthritis and Pathology

    doi: 10.1128/mBio.01456-17

    Figure Lengend Snippet: Cytokine gene expression is reduced during CHIKV Opal524R infection. (A, B) Twenty-four-day-old C57BL6/J mice were infected in the left rear footpad with 100 PFU of wild-type CHIKV ( n = 5) or Opal524R ( n = 5) or mock infected with diluent ( n = 3). At days 1 (A) and 2 (B) postinfection, mice were sacrificed and perfused intracardially with 1× PBS, and the ipsilateral foot was harvested in TRIzol. RNA was extracted, cDNA was generated, and cytokine gene expression was assessed by quantitative real-time PCR. Each cytokine was normalized to host 18S gene expression. Fold changes in gene expression relative to mock infection (left) and wild-type infection (right) are shown. Data are representative of two independent experiments. Statistical significance is indicated as follows: *, P

    Article Snippet: RNA was isolated from purified virus preparations by TRIzol extraction (Ambion).

    Techniques: Expressing, Infection, Mouse Assay, Generated, Real-time Polymerase Chain Reaction

    Recovery of nucleic acids and polyphosphate (polyP) using various purification methods. Known quantities of bacteriophage lambda DNA (DNA, striped bars), polyG (RNA, white bars), or long-chain polyP (black bars) were processed for nucleic acid purification using Qiaquick, DNeasy, or TRIzol kits, following the manufacturer’s instructions, or with phenol/chloroform extraction. Nucleic acid recovery was quantified using A 260 and polyP recovery by digestion with PPX1 and PiBlue assay. Results are plotted as percent of the input amount in each experiment. For all experiments except the Qiaquick kits, the three samples were: (A) DNA alone (5 µg lambda DNA); (B) RNA alone (5 µg polyG); (C) 5 µg polyP alone; (D) a mixture of 5 µg polyP plus 5 µg of DNA; or (E) 5 µg polyP plus 5 µg of RNA (E) . Experiments with the Qiaquick kit employed 2.5 µg of polyP and/or nucleic acid. Data are mean ± SEM ( n = 3).

    Journal: Frontiers in Medicine

    Article Title: Ability of Polyphosphate and Nucleic Acids to Trigger Blood Clotting: Some Observations and Caveats

    doi: 10.3389/fmed.2018.00107

    Figure Lengend Snippet: Recovery of nucleic acids and polyphosphate (polyP) using various purification methods. Known quantities of bacteriophage lambda DNA (DNA, striped bars), polyG (RNA, white bars), or long-chain polyP (black bars) were processed for nucleic acid purification using Qiaquick, DNeasy, or TRIzol kits, following the manufacturer’s instructions, or with phenol/chloroform extraction. Nucleic acid recovery was quantified using A 260 and polyP recovery by digestion with PPX1 and PiBlue assay. Results are plotted as percent of the input amount in each experiment. For all experiments except the Qiaquick kits, the three samples were: (A) DNA alone (5 µg lambda DNA); (B) RNA alone (5 µg polyG); (C) 5 µg polyP alone; (D) a mixture of 5 µg polyP plus 5 µg of DNA; or (E) 5 µg polyP plus 5 µg of RNA (E) . Experiments with the Qiaquick kit employed 2.5 µg of polyP and/or nucleic acid. Data are mean ± SEM ( n = 3).

    Article Snippet: In these experiments, known quantities of purified bacteriophage lambda DNA or a synthetic RNA homopolymer, polyG, either alone or mixed with long-chain polyP, were purified using phenol/chloroform extraction or Qiaquick kits (Qiagen), DNeasy kits (Qiagen), RNeasy Miniprep kits (Qiagen), or TRIzol kits (Thermo Fisher), following the manufacturers’ instructions.

    Techniques: Purification, Lambda DNA Preparation, Nucleic Acid Purification

    Simultaneous optimization of EDTA, NaCl, and SDS concentrations enhances protein yield during solubilization of TRIzol-precipitated protein

    Journal: Journal of neuroscience methods

    Article Title: Optimized solubilization of TRIzol-precipitated protein permits Western blotting analysis to maximize data available from brain tissue

    doi: 10.1016/j.jneumeth.2017.02.002

    Figure Lengend Snippet: Simultaneous optimization of EDTA, NaCl, and SDS concentrations enhances protein yield during solubilization of TRIzol-precipitated protein

    Article Snippet: In fact, the TRIzol protocol (Life Technologies) recommends solubilization in 1% SDS, and indicates that dialysis and subsequent reconstitution in an SDS-urea solution may improve yield (Life Technologies; November 9, 2016 edition).

    Techniques:

    3.1 EDTA, NaCl, and SDS concentrations modulate protein yield during solubilization of TRIzol-precipitated protein

    Journal: Journal of neuroscience methods

    Article Title: Optimized solubilization of TRIzol-precipitated protein permits Western blotting analysis to maximize data available from brain tissue

    doi: 10.1016/j.jneumeth.2017.02.002

    Figure Lengend Snippet: 3.1 EDTA, NaCl, and SDS concentrations modulate protein yield during solubilization of TRIzol-precipitated protein

    Article Snippet: In fact, the TRIzol protocol (Life Technologies) recommends solubilization in 1% SDS, and indicates that dialysis and subsequent reconstitution in an SDS-urea solution may improve yield (Life Technologies; November 9, 2016 edition).

    Techniques:

    EDTA, NaCl, and SDS concentrations significantly alter protein yield during solubilization of TRIzol-precipitated protein

    Journal: Journal of neuroscience methods

    Article Title: Optimized solubilization of TRIzol-precipitated protein permits Western blotting analysis to maximize data available from brain tissue

    doi: 10.1016/j.jneumeth.2017.02.002

    Figure Lengend Snippet: EDTA, NaCl, and SDS concentrations significantly alter protein yield during solubilization of TRIzol-precipitated protein

    Article Snippet: In fact, the TRIzol protocol (Life Technologies) recommends solubilization in 1% SDS, and indicates that dialysis and subsequent reconstitution in an SDS-urea solution may improve yield (Life Technologies; November 9, 2016 edition).

    Techniques:

    Selective induction of BRLF1 and BZLF1 expression by inducing chemicals in B95-8 and HH514-16 cells. (A) Induction of mRNAs containing BRLF1 and BZLF1 . At 20 h after treatment of the cells with TPA or n -butyrate, singly or in combination, RNA was prepared by the TRIzol method. RNA from 3 × 10 6 cells per lane was analyzed by Northern blotting with a probe prepared from a portion of the BZLF1 cDNA. This probe detects the 4.0- and 3.0-kb bicistronic ( BRLF1 plus BZLF1 ) mRNA from Rp and the 1.0-kb monocistronic ( BZLF1 ). The blot was reprobed with β-actin to control for loading of RNA. (B) Comparison of the effects of n -butyrate (B) and TSA. Cytoplasmic RNA prepared 17 h after chemical treatment was analyzed by Northern blotting with the BZLF1 probe. The RNA blots were also probed for β-actin and the H1 RNA component of human RNase P to control for RNA loading.

    Journal: Journal of Virology

    Article Title: Protein Kinase C-Independent Activation of the Epstein-Barr Virus Lytic Cycle

    doi: 10.1128/JVI.76.11.5612-5626.2002

    Figure Lengend Snippet: Selective induction of BRLF1 and BZLF1 expression by inducing chemicals in B95-8 and HH514-16 cells. (A) Induction of mRNAs containing BRLF1 and BZLF1 . At 20 h after treatment of the cells with TPA or n -butyrate, singly or in combination, RNA was prepared by the TRIzol method. RNA from 3 × 10 6 cells per lane was analyzed by Northern blotting with a probe prepared from a portion of the BZLF1 cDNA. This probe detects the 4.0- and 3.0-kb bicistronic ( BRLF1 plus BZLF1 ) mRNA from Rp and the 1.0-kb monocistronic ( BZLF1 ). The blot was reprobed with β-actin to control for loading of RNA. (B) Comparison of the effects of n -butyrate (B) and TSA. Cytoplasmic RNA prepared 17 h after chemical treatment was analyzed by Northern blotting with the BZLF1 probe. The RNA blots were also probed for β-actin and the H1 RNA component of human RNase P to control for RNA loading.

    Article Snippet: Total RNA was prepared with RNeasy and Qia-shredder spin columns (Qiagen) or TRIzol reagent (GIBCO/BRL) according to the manufacturers' directions.

    Techniques: Expressing, Northern Blot