Journal: Frontiers in Microbiology

Article Title: The MtrCDE Efflux Pump Contributes to Survival of Neisseria gonorrhoeae From Human Neutrophils and Their Antimicrobial Components

doi: 10.3389/fmicb.2018.02688

Figure Lengend Snippet: Characterization of MtrCDE efflux pump mutants in piliated, opacity protein-deficient Gc of strain FA1090. (A) “Opaless” parent, ΔmtrC, ΔmtrD, ΔmtrE , and mtrE complement ( mtrE + ) Gc were exposed to increasing concentrations of Triton X-100 or vehicle control for 1 h. Bacterial survival was calculated as the CFU enumerated after 1 h divided by CFU at the time of inoculation, expressed relative to survival in the vehicle control (100%). Data are presented as the mean ± SEM for 3–4 biological replicates. ∗ P ≤ 0.05 for parent vs. each mtr mutant at the indicated concentration (one-way ANOVA followed by Tukey’s multiple comparisons test). (B) Minimal inhibitory concentrations (MIC) for erythromycin were calculated for parent, ΔmtrC, ΔmtrD, ΔmtrE , and mtrE + Gc. Data are presented as the geometric mean MIC ± SD for 3–11 biological replicates. ∗ P

Article Snippet: Triton X-100 Triton X-100 (Sigma) was diluted in ddH2 O. Gc was incubated in Triton X-100 in 0.5x GCBL for 1 h.

Techniques: Mutagenesis, Concentration Assay

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

Article Title: Vascular Endothelial Cells Mediate Mechanical Stimulation-Induced Enhancement of Endothelin Hyperalgesia via Activation of P2X2/3 Receptors on Nociceptors

doi: 10.1523/JNEUROSCI.3229-12.2013

Figure Lengend Snippet: Effect of octoxynol-9 on platelet activating factor-induced plasma protein extravasation

Article Snippet: The drugs employed in this study were: endothelin-1 (ET-1, 100 ng), BQ-123, 1 μg (ETA receptor antagonist), BQ-788, 1 μg (ETB receptor antagonist), TMB-8, 1 μg (calcium sequestrator), Quin-2, 1 μg (calcium chelator), oligomycin and α-lipoic acid (reactive oxygen species (ROS) inhibitors), rotenone, 1 μg and antimycin, 1 μg (inhibitors of mitochondrial electron transport chain complexes I & III, respectively), A-317491, 1 μg (P2X2/3 inhibitor), octoxynol-9 (which functionally impairs vasculature endothelial cell lining ( ; ; ), oxotremorine and platelet activating factor (all from Sigma-Aldrich, St. Louis, MO), bisindolylmaleimide (BIMM, a non-selective PKC inhibitor) and PKCεV1–2 peptide (PKCεI, a selective PKCε-translocation inhibitor; both from Calbiochem, EMD Biosciences, Inc. La Jolla, CA).

Techniques:

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

Article Title: Vascular Endothelial Cells Mediate Mechanical Stimulation-Induced Enhancement of Endothelin Hyperalgesia via Activation of P2X2/3 Receptors on Nociceptors

doi: 10.1523/JNEUROSCI.3229-12.2013

Figure Lengend Snippet: Oxotremorine-induced an increase in blood flow attenuated by treatment with octoxynol-9

Article Snippet: The drugs employed in this study were: endothelin-1 (ET-1, 100 ng), BQ-123, 1 μg (ETA receptor antagonist), BQ-788, 1 μg (ETB receptor antagonist), TMB-8, 1 μg (calcium sequestrator), Quin-2, 1 μg (calcium chelator), oligomycin and α-lipoic acid (reactive oxygen species (ROS) inhibitors), rotenone, 1 μg and antimycin, 1 μg (inhibitors of mitochondrial electron transport chain complexes I & III, respectively), A-317491, 1 μg (P2X2/3 inhibitor), octoxynol-9 (which functionally impairs vasculature endothelial cell lining ( ; ; ), oxotremorine and platelet activating factor (all from Sigma-Aldrich, St. Louis, MO), bisindolylmaleimide (BIMM, a non-selective PKC inhibitor) and PKCεV1–2 peptide (PKCεI, a selective PKCε-translocation inhibitor; both from Calbiochem, EMD Biosciences, Inc. La Jolla, CA).

Techniques: Flow Cytometry

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

Article Title: Vascular Endothelial Cells Mediate Mechanical Stimulation-Induced Enhancement of Endothelin Hyperalgesia via Activation of P2X2/3 Receptors on Nociceptors

doi: 10.1523/JNEUROSCI.3229-12.2013

Figure Lengend Snippet: Effect of octoxynol-9 on ET-1 induced mechanical hyperalgesia and SIEH

Article Snippet: The drugs employed in this study were: endothelin-1 (ET-1, 100 ng), BQ-123, 1 μg (ETA receptor antagonist), BQ-788, 1 μg (ETB receptor antagonist), TMB-8, 1 μg (calcium sequestrator), Quin-2, 1 μg (calcium chelator), oligomycin and α-lipoic acid (reactive oxygen species (ROS) inhibitors), rotenone, 1 μg and antimycin, 1 μg (inhibitors of mitochondrial electron transport chain complexes I & III, respectively), A-317491, 1 μg (P2X2/3 inhibitor), octoxynol-9 (which functionally impairs vasculature endothelial cell lining ( ; ; ), oxotremorine and platelet activating factor (all from Sigma-Aldrich, St. Louis, MO), bisindolylmaleimide (BIMM, a non-selective PKC inhibitor) and PKCεV1–2 peptide (PKCεI, a selective PKCε-translocation inhibitor; both from Calbiochem, EMD Biosciences, Inc. La Jolla, CA).

Techniques:

Journal: MethodsX

Article Title: Colorimetric quantification of sucrose in presence of thermo-sensitive polymers present in aqueous two-phase systems

doi: 10.1016/j.mex.2014.09.006

Figure Lengend Snippet: Standard curves of phenol–sulfuric acid method conducted at an ice-cold temperature (A) and room temperature (B). (A) -×-: sucrose; ⋯+⋯: sucrose + Triton X-114 (0.05%, w/v); ⋯□⋯: sucrose + Triton X-114 (0.5%, w/v); ⋯○⋯: sucrose + Triton X-114 (5%, w/v); (B) -♦-: sucrose; -■-: sucrose + Triton X-114 (0.5%, w/v); -▴- sucrose + Triton X-100 (0.5%, w/v); -●-: sucrose + EO50PO50 (0.5%, w/v)

Article Snippet: Materials • EO50PO50 (Sigma, Cat. No. 438189) • Phenol (Scharlau, Cat. No. FE04821000) • Sucrose (Sigma, Cat. No. S5106) • Sulfuric acid (Merck, Cat. No. 1.00731.1000) • Triton X-100 (Sigma, Cat. No. T9284) • Triton X-114 (Sigma, Cat. No. X144) Ultra-pure water was used throughout the experiment.

Techniques:

Journal: Molecules and Cells

Article Title: Mass Spectrometry-Based Screening Platform Reveals Orco Interactome in Drosophila melanogaster

doi: 10.14348/molcells.2018.2305

Figure Lengend Snippet: Detergents and temperature affect Orco solubility (A) Various laboratory detergents solubilizing Orco from enriched tissues. All detergents are at 1% (v/v or w/v). T20, Tween 20; TX100, Triton X-100; NP40, Nonidet P-40; OG, Octyl-β-D-glucopyranoside; DDM, n-Dodecyl β-D-maltoside; Z3–16, Zwittergent 3–16; SDS, sodium dodecyl sulfate. (B) Temperature sensitivity of Orco samples prepared from a 1% DDM lysate in the presence of SDS and β-mercaptoethanol. Antibodies are against EGFP and native GAPDH.

Article Snippet: The detergents used were Tween 20 (Biosesang, T1027), Triton X-100 (Bio Basic, TB0198), Nonidet P-40 (Bio Basic, NDB0385), Octyl-β-D-glucopyranoside (Fluka, 75083), n-Dodecyl β-D-maltoside (DDM; ThermoFisher, 89903), CHAPS (Sigma-Aldrich, C5070), CHAPSO (Calbiochem, 220201), and Zwittergent 3–16 (Calbiochem, 693023).

Techniques: Solubility