triticum vulgaris wga Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Vector Laboratories triticum vulgaris agglutinin
    Triticum Vulgaris Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triticum vulgaris agglutinin/product/Vector Laboratories
    Average 94 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    triticum vulgaris agglutinin - by Bioz Stars, 2021-01
    94/100 stars
      Buy from Supplier

    99
    Millipore wga
    <t>WGA-HRP-labeled</t> motor neurons in lumbosacral cord and sensory neurons in DRG. (A) Lumbosacral spinal cord from sham-infected hamster, and from (B) WNV-infected hamster. (C) DRG of L5 from sham-infected hamster, and from (D) WNV-infected hamster. Hamsters were injected in the sciatic nerve with WNV, followed by injection in the sciatic nerve with WGA-HRP 9 days later, and necropsied 3 days later (12 days after WNV infection) for WGA-HRP staining of the lumbosacral spinal cord and the ipsilateral DRG. Graphs are the respective quantitative the number of motor neurons labeled with WGA-HRP in lumbosacral spinal cord and sensory neurons in DRG. The numbers of WNV-infected hamsters were less than the sham-infected hamsters, because not all WNV-infected hamsters survived for analysis. Scale bar 100 μm.
    Wga, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 598 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wga/product/Millipore
    Average 99 stars, based on 598 article reviews
    Price from $9.99 to $1999.99
    wga - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    93
    Millipore anti lectin triticum vulgaris antibody
    <t>WGA-HRP-labeled</t> motor neurons in lumbosacral cord and sensory neurons in DRG. (A) Lumbosacral spinal cord from sham-infected hamster, and from (B) WNV-infected hamster. (C) DRG of L5 from sham-infected hamster, and from (D) WNV-infected hamster. Hamsters were injected in the sciatic nerve with WNV, followed by injection in the sciatic nerve with WGA-HRP 9 days later, and necropsied 3 days later (12 days after WNV infection) for WGA-HRP staining of the lumbosacral spinal cord and the ipsilateral DRG. Graphs are the respective quantitative the number of motor neurons labeled with WGA-HRP in lumbosacral spinal cord and sensory neurons in DRG. The numbers of WNV-infected hamsters were less than the sham-infected hamsters, because not all WNV-infected hamsters survived for analysis. Scale bar 100 μm.
    Anti Lectin Triticum Vulgaris Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti lectin triticum vulgaris antibody/product/Millipore
    Average 93 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    anti lectin triticum vulgaris antibody - by Bioz Stars, 2021-01
    93/100 stars
      Buy from Supplier

    97
    Vector Laboratories triticum vulgaris agglutinin wga
    <t>WGA-HRP-labeled</t> motor neurons in lumbosacral cord and sensory neurons in DRG. (A) Lumbosacral spinal cord from sham-infected hamster, and from (B) WNV-infected hamster. (C) DRG of L5 from sham-infected hamster, and from (D) WNV-infected hamster. Hamsters were injected in the sciatic nerve with WNV, followed by injection in the sciatic nerve with WGA-HRP 9 days later, and necropsied 3 days later (12 days after WNV infection) for WGA-HRP staining of the lumbosacral spinal cord and the ipsilateral DRG. Graphs are the respective quantitative the number of motor neurons labeled with WGA-HRP in lumbosacral spinal cord and sensory neurons in DRG. The numbers of WNV-infected hamsters were less than the sham-infected hamsters, because not all WNV-infected hamsters survived for analysis. Scale bar 100 μm.
    Triticum Vulgaris Agglutinin Wga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triticum vulgaris agglutinin wga/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triticum vulgaris agglutinin wga - by Bioz Stars, 2021-01
    97/100 stars
      Buy from Supplier

    85
    EY Laboratories triticum vulgaris lectin wga
    <t>WGA-HRP-labeled</t> motor neurons in lumbosacral cord and sensory neurons in DRG. (A) Lumbosacral spinal cord from sham-infected hamster, and from (B) WNV-infected hamster. (C) DRG of L5 from sham-infected hamster, and from (D) WNV-infected hamster. Hamsters were injected in the sciatic nerve with WNV, followed by injection in the sciatic nerve with WGA-HRP 9 days later, and necropsied 3 days later (12 days after WNV infection) for WGA-HRP staining of the lumbosacral spinal cord and the ipsilateral DRG. Graphs are the respective quantitative the number of motor neurons labeled with WGA-HRP in lumbosacral spinal cord and sensory neurons in DRG. The numbers of WNV-infected hamsters were less than the sham-infected hamsters, because not all WNV-infected hamsters survived for analysis. Scale bar 100 μm.
    Triticum Vulgaris Lectin Wga, supplied by EY Laboratories, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triticum vulgaris lectin wga/product/EY Laboratories
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    triticum vulgaris lectin wga - by Bioz Stars, 2021-01
    85/100 stars
      Buy from Supplier

    90
    Megazyme triticum vulgaris wga
    <t>WGA-HRP-labeled</t> motor neurons in lumbosacral cord and sensory neurons in DRG. (A) Lumbosacral spinal cord from sham-infected hamster, and from (B) WNV-infected hamster. (C) DRG of L5 from sham-infected hamster, and from (D) WNV-infected hamster. Hamsters were injected in the sciatic nerve with WNV, followed by injection in the sciatic nerve with WGA-HRP 9 days later, and necropsied 3 days later (12 days after WNV infection) for WGA-HRP staining of the lumbosacral spinal cord and the ipsilateral DRG. Graphs are the respective quantitative the number of motor neurons labeled with WGA-HRP in lumbosacral spinal cord and sensory neurons in DRG. The numbers of WNV-infected hamsters were less than the sham-infected hamsters, because not all WNV-infected hamsters survived for analysis. Scale bar 100 μm.
    Triticum Vulgaris Wga, supplied by Megazyme, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triticum vulgaris wga/product/Megazyme
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triticum vulgaris wga - by Bioz Stars, 2021-01
    90/100 stars
      Buy from Supplier


    Image Search Results


    WGA-HRP-labeled motor neurons in lumbosacral cord and sensory neurons in DRG. (A) Lumbosacral spinal cord from sham-infected hamster, and from (B) WNV-infected hamster. (C) DRG of L5 from sham-infected hamster, and from (D) WNV-infected hamster. Hamsters were injected in the sciatic nerve with WNV, followed by injection in the sciatic nerve with WGA-HRP 9 days later, and necropsied 3 days later (12 days after WNV infection) for WGA-HRP staining of the lumbosacral spinal cord and the ipsilateral DRG. Graphs are the respective quantitative the number of motor neurons labeled with WGA-HRP in lumbosacral spinal cord and sensory neurons in DRG. The numbers of WNV-infected hamsters were less than the sham-infected hamsters, because not all WNV-infected hamsters survived for analysis. Scale bar 100 μm.

    Journal: Journal of neurovirology

    Article Title: West Nile virus preferentially transports along motor neuron axons after sciatic nerve injection of hamsters

    doi: 10.1080/13550280902973978

    Figure Lengend Snippet: WGA-HRP-labeled motor neurons in lumbosacral cord and sensory neurons in DRG. (A) Lumbosacral spinal cord from sham-infected hamster, and from (B) WNV-infected hamster. (C) DRG of L5 from sham-infected hamster, and from (D) WNV-infected hamster. Hamsters were injected in the sciatic nerve with WNV, followed by injection in the sciatic nerve with WGA-HRP 9 days later, and necropsied 3 days later (12 days after WNV infection) for WGA-HRP staining of the lumbosacral spinal cord and the ipsilateral DRG. Graphs are the respective quantitative the number of motor neurons labeled with WGA-HRP in lumbosacral spinal cord and sensory neurons in DRG. The numbers of WNV-infected hamsters were less than the sham-infected hamsters, because not all WNV-infected hamsters survived for analysis. Scale bar 100 μm.

    Article Snippet: Nine days after the sciatic nerve injection, the sciatic nerve in the great sciatic notch was surgically exposed and injected with 1 μL of 5% WGA-HRP (Sigma-Aldrich, L7017) ( ).

    Techniques: Whole Genome Amplification, Labeling, Infection, Injection, Staining

    Role of p38/MAPK activation in MAP4 phosphorylation-induced cardiac hypertrophy, apoptosis and fibrosis. (a) Detection of p-M and p-P38 with or without SB203580 (SB) (5 μM) in CMs. n = 5. (b) Protein markers of hypertrophy and p-P38 were detected using WB. n = 5. (c) Cross sectional areas of CMs were analyzed using cTnI and FITC-WGA co-staining. n = 5. (d and e) CMs apoptosis was detected by cTnI and fluorescein-TUNEL co-staining. Bar, 10 μm. n = 5. (f) Protein levels of caspase-3 and t-caspase-3 were analyzed using WB. n = 5. (g) Detection of Cyt-c release from mitochondria. n = 5. (h) CMs mitochondria observed by TEM (black square indicated representative mitochondria). Bar, 1 μm. (i) Markers of ECM were examined using WB. n = 5. (j) Poly/free tubulin was analyzed using WB. n = 5. (k) Representative confocal immunofluorescence images showing the MTs organization of the CMs. Bar, 10 μm. The inserts showed high magnification images of the peripheral MT network. (l) Detection of p-M translocation to mitochondria with or without SB. n = 5. (m and n) CMs apoptosis was detected by cTnI and fluorescein-TUNEL co-staining after the cells were transiently transfected with MAP4(Ala), MKK6(Glu) or both. Bar, 10 μm. n = 5. (o) Representative confocal immunofluorescence images showing the MTs organization of the CMs after the cells were transiently transfected with MAP4(Ala), MKK6(Glu) or both. Bar, 10 μm. The inserts showed high magnification images of the peripheral MT network. (p) CMs mitochondria observed by TEM (black square indicated representative mitochondria) after the cells were transiently transfected with MAP4(Ala), MKK6(Glu) or both. Bar, 1 μm. Data were shown as mean ± SEM. The experiment was conducted 5 times. *P

    Journal: EBioMedicine

    Article Title: Microtubule associated protein 4 phosphorylation leads to pathological cardiac remodeling in mice

    doi: 10.1016/j.ebiom.2018.10.017

    Figure Lengend Snippet: Role of p38/MAPK activation in MAP4 phosphorylation-induced cardiac hypertrophy, apoptosis and fibrosis. (a) Detection of p-M and p-P38 with or without SB203580 (SB) (5 μM) in CMs. n = 5. (b) Protein markers of hypertrophy and p-P38 were detected using WB. n = 5. (c) Cross sectional areas of CMs were analyzed using cTnI and FITC-WGA co-staining. n = 5. (d and e) CMs apoptosis was detected by cTnI and fluorescein-TUNEL co-staining. Bar, 10 μm. n = 5. (f) Protein levels of caspase-3 and t-caspase-3 were analyzed using WB. n = 5. (g) Detection of Cyt-c release from mitochondria. n = 5. (h) CMs mitochondria observed by TEM (black square indicated representative mitochondria). Bar, 1 μm. (i) Markers of ECM were examined using WB. n = 5. (j) Poly/free tubulin was analyzed using WB. n = 5. (k) Representative confocal immunofluorescence images showing the MTs organization of the CMs. Bar, 10 μm. The inserts showed high magnification images of the peripheral MT network. (l) Detection of p-M translocation to mitochondria with or without SB. n = 5. (m and n) CMs apoptosis was detected by cTnI and fluorescein-TUNEL co-staining after the cells were transiently transfected with MAP4(Ala), MKK6(Glu) or both. Bar, 10 μm. n = 5. (o) Representative confocal immunofluorescence images showing the MTs organization of the CMs after the cells were transiently transfected with MAP4(Ala), MKK6(Glu) or both. Bar, 10 μm. The inserts showed high magnification images of the peripheral MT network. (p) CMs mitochondria observed by TEM (black square indicated representative mitochondria) after the cells were transiently transfected with MAP4(Ala), MKK6(Glu) or both. Bar, 1 μm. Data were shown as mean ± SEM. The experiment was conducted 5 times. *P

    Article Snippet: While for cardiac troponin I (cTnI) (Proteintech Group Cat# 21652-1-AP, 1:50) and FITC- wheat germ agglutinins (WGA) (Sigma, Cat# L4895, 20 μg/ml) or terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) co-staining, the cells were incubated with cTnI at 4 °C overnight and then co-incubated with secondary antibodies conjugated to Cy3 and FITC-WGA or fluorescein-TUNEL reaction mixture for 1 h at 37 °C, and then the nuclei were stained for 2 min with DAPI.

    Techniques: Activation Assay, Western Blot, Whole Genome Amplification, Staining, TUNEL Assay, Transmission Electron Microscopy, Immunofluorescence, Translocation Assay, Transfection

    Serpina3n overexpression protects against sarcolemma membrane damage. ( A and B ) Representative histological images and quantitation of EBD (red) myofiber staining in the TA muscle following running in the indicated genotypes of mice. WGA-FITC (green) is used as a membrane marker. The number of mice used is shown in the graph. * P

    Journal: Human Molecular Genetics

    Article Title: Genetic overexpression of Serpina3n attenuates muscular dystrophy in mice

    doi: 10.1093/hmg/ddw005

    Figure Lengend Snippet: Serpina3n overexpression protects against sarcolemma membrane damage. ( A and B ) Representative histological images and quantitation of EBD (red) myofiber staining in the TA muscle following running in the indicated genotypes of mice. WGA-FITC (green) is used as a membrane marker. The number of mice used is shown in the graph. * P

    Article Snippet: Wheat germ agglutinin-fluorescein isothiocyanate (WGA-FITC) (Sigma-Aldrich; L4895) was used at 50 μg/ml to show fiber outlines in the EBD immunohistochemistry experiments and Wheat germ agglutinin-tetramethylrhodamine (Sigma-Aldrich; L5266) was used at 100 μg/ml in the images with CD45 and Mac3 staining.

    Techniques: Over Expression, Quantitation Assay, Staining, Mouse Assay, Whole Genome Amplification, Marker

    A chitin-like component masks the immunorecognition of β-glucans on sclerotic cells by Dectin-1. (A1–A4) The binding of murine-derived Dectin-1 to the saprophytic F. pedrosoi in PDB before (A1) and after (A4) β-glucanase treatment, or to the 30-day (A2) and 50-day (A3) induced cultures containing sclerotic cells in ATCC medium 830 was detected by PE-anti-murine Dectin-1 mAb using confocal microscope. (B1 and B2) The binding of human-derived Dectin-1 to the induced cultures containing sclerotic cells was detected by PE-anti-human Dectin-1 mAb using confocal microscope. (C) The binding of murine-derived mannose receptor (MR) to the induced cultures containing sclerotic cells and chlamydospores was detected by FITC-anti-murine MR using confocal microscope. (D) FITC-WGA was used to detect the distribution of chitin-like component on the induced cultures containing sclerotic cells before (D1) and after (D2) Chitinase treatment using confocal microscope. (E1 and E2) The binding capacity of FITC-WGA to the saprophytic F. pedrosoi or to the induced cultures before and after chitinase treatment was detected using flow cytometry assay, and represented as the Mean Fluorescence Intensity (MFI). The induced cultures without any treatment were set as blank control. (F1 and F2) SEM was used to observe the surface ultra-structure of saprophytic hypha (F1) and in vitro-transformed sclerotic cells (F2) in the presence or absence of chitinase pretreatment. SEM, Scale bar = 4 µm (left) or 500 nm (middle and right). (G1 and G2) The binding of murine-derived Dectin-1 to the induced cultures containing sclerotic cells with chitinase pretreatment was detected by confocal microscope. (A-D, G) The transformed sclerotic cells and chlamydospores were indicated by red arrows. (H1 and H2) The binding capacity of murine-derived Dectin-1 to the saprophytic F. pedrosoi before and after β-glucanase treatment, or to the induced cultures before and after chitinase treatment was detected by indirect immunofluorescence assay using flow cytometer, and represented as the Mean Fluorescence Intensity (MFI). The fungal cells only incubated with PE-conjugated anti-murine Dectin-1 mAb were set as blank control. (E2 and H2) Data are shown as mean ± SEM from three individual experiments performed in triplicates, and significance tested using LSD-t test.

    Journal: PLoS ONE

    Article Title: A Chitin-Like Component on Sclerotic Cells of Fonsecaea pedrosoi Inhibits Dectin-1-Mediated Murine Th17 Development by Masking β-Glucans

    doi: 10.1371/journal.pone.0114113

    Figure Lengend Snippet: A chitin-like component masks the immunorecognition of β-glucans on sclerotic cells by Dectin-1. (A1–A4) The binding of murine-derived Dectin-1 to the saprophytic F. pedrosoi in PDB before (A1) and after (A4) β-glucanase treatment, or to the 30-day (A2) and 50-day (A3) induced cultures containing sclerotic cells in ATCC medium 830 was detected by PE-anti-murine Dectin-1 mAb using confocal microscope. (B1 and B2) The binding of human-derived Dectin-1 to the induced cultures containing sclerotic cells was detected by PE-anti-human Dectin-1 mAb using confocal microscope. (C) The binding of murine-derived mannose receptor (MR) to the induced cultures containing sclerotic cells and chlamydospores was detected by FITC-anti-murine MR using confocal microscope. (D) FITC-WGA was used to detect the distribution of chitin-like component on the induced cultures containing sclerotic cells before (D1) and after (D2) Chitinase treatment using confocal microscope. (E1 and E2) The binding capacity of FITC-WGA to the saprophytic F. pedrosoi or to the induced cultures before and after chitinase treatment was detected using flow cytometry assay, and represented as the Mean Fluorescence Intensity (MFI). The induced cultures without any treatment were set as blank control. (F1 and F2) SEM was used to observe the surface ultra-structure of saprophytic hypha (F1) and in vitro-transformed sclerotic cells (F2) in the presence or absence of chitinase pretreatment. SEM, Scale bar = 4 µm (left) or 500 nm (middle and right). (G1 and G2) The binding of murine-derived Dectin-1 to the induced cultures containing sclerotic cells with chitinase pretreatment was detected by confocal microscope. (A-D, G) The transformed sclerotic cells and chlamydospores were indicated by red arrows. (H1 and H2) The binding capacity of murine-derived Dectin-1 to the saprophytic F. pedrosoi before and after β-glucanase treatment, or to the induced cultures before and after chitinase treatment was detected by indirect immunofluorescence assay using flow cytometer, and represented as the Mean Fluorescence Intensity (MFI). The fungal cells only incubated with PE-conjugated anti-murine Dectin-1 mAb were set as blank control. (E2 and H2) Data are shown as mean ± SEM from three individual experiments performed in triplicates, and significance tested using LSD-t test.

    Article Snippet: In addition, FITC-conjugated Wheat Germ Agglutinin (WGA) was used according to the manufacturer's protocol (L4895, Sigma-aldrich) to detect the distribution of chitin moiety on fungal cells.

    Techniques: Binding Assay, Derivative Assay, Microscopy, Whole Genome Amplification, Flow Cytometry, Cytometry, Fluorescence, In Vitro, Transformation Assay, Immunofluorescence, Incubation

    ESL of mesenteric microvessels is compromised in old MWF rats. Confocal microscopy images of FITC-WGA lectin (green) labeling ESL (esl), alongside simultaneous DIC imaging of microvessel anatomy in a healthy young MWF rat (A and B) and an old MWF rat

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Loss of the Endothelial Glycocalyx Links Albuminuria and Vascular Dysfunction

    doi: 10.1681/ASN.2012010017

    Figure Lengend Snippet: ESL of mesenteric microvessels is compromised in old MWF rats. Confocal microscopy images of FITC-WGA lectin (green) labeling ESL (esl), alongside simultaneous DIC imaging of microvessel anatomy in a healthy young MWF rat (A and B) and an old MWF rat

    Article Snippet: FITC-labeled wheat germ agglutinin (FITC-WGA) lectin (from Triticum vulgaris ; 6.25 mg/kg body wt; L4895; Sigma, MO), administered via the carotid cannula approximately 30 minutes before imaging, was used to label N-acetyl glucosamine (a component of heparan sulfate and hyaluronan glycosaminoglycans) and N-acetyl neuraminic acid (a major sialic acid present on the endothelial cell surface) oligosaccharide moieties on the endothelial cell surface.

    Techniques: Confocal Microscopy, Whole Genome Amplification, Labeling, Imaging

    ESL of glomerular capillaries is compromised in older MWF rats. (A) Under baseline conditions, multiphoton microscopy imaging reveals a linear label (white arrows) of FITC-WGA lectin (green) at the margin of superficial glomerular capillaries containing

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Loss of the Endothelial Glycocalyx Links Albuminuria and Vascular Dysfunction

    doi: 10.1681/ASN.2012010017

    Figure Lengend Snippet: ESL of glomerular capillaries is compromised in older MWF rats. (A) Under baseline conditions, multiphoton microscopy imaging reveals a linear label (white arrows) of FITC-WGA lectin (green) at the margin of superficial glomerular capillaries containing

    Article Snippet: FITC-labeled wheat germ agglutinin (FITC-WGA) lectin (from Triticum vulgaris ; 6.25 mg/kg body wt; L4895; Sigma, MO), administered via the carotid cannula approximately 30 minutes before imaging, was used to label N-acetyl glucosamine (a component of heparan sulfate and hyaluronan glycosaminoglycans) and N-acetyl neuraminic acid (a major sialic acid present on the endothelial cell surface) oligosaccharide moieties on the endothelial cell surface.

    Techniques: Microscopy, Imaging, Whole Genome Amplification