tris-hcl New England Biolabs Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    New England Biolabs casein kinase ii ckii buffer buffer 20 mm tris hcl
    Casein Kinase Ii Ckii Buffer Buffer 20 Mm Tris Hcl, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/casein kinase ii ckii buffer buffer 20 mm tris hcl/product/New England Biolabs
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    casein kinase ii ckii buffer buffer 20 mm tris hcl - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs tris hcl
    Tris Hcl, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris hcl/product/New England Biolabs
    Average 95 stars, based on 200 article reviews
    Price from $9.99 to $1999.99
    tris hcl - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    90
    New England Biolabs thermopol buffer
    Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× <t>ThermoPol</t> buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.
    Thermopol Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1722 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermopol buffer/product/New England Biolabs
    Average 90 stars, based on 1722 article reviews
    Price from $9.99 to $1999.99
    thermopol buffer - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    95
    New England Biolabs nebuffer 3
    Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× <t>ThermoPol</t> buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.
    Nebuffer 3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 430 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebuffer 3/product/New England Biolabs
    Average 95 stars, based on 430 article reviews
    Price from $9.99 to $1999.99
    nebuffer 3 - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs nebuffer 2
    Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× <t>ThermoPol</t> buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.
    Nebuffer 2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebuffer 2/product/New England Biolabs
    Average 95 stars, based on 1520 article reviews
    Price from $9.99 to $1999.99
    nebuffer 2 - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs t4 polynucleotide kinase buffer
    Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× <t>ThermoPol</t> buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.
    T4 Polynucleotide Kinase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 polynucleotide kinase buffer/product/New England Biolabs
    Average 95 stars, based on 214 article reviews
    Price from $9.99 to $1999.99
    t4 polynucleotide kinase buffer - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs standard taq buffer
    Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× <t>ThermoPol</t> buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.
    Standard Taq Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 363 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard taq buffer/product/New England Biolabs
    Average 95 stars, based on 363 article reviews
    Price from $9.99 to $1999.99
    standard taq buffer - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs ne buffer 1
    Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× <t>ThermoPol</t> buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.
    Ne Buffer 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ne buffer 1/product/New England Biolabs
    Average 95 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    ne buffer 1 - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs t7 rna polymerase buffer
    Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× <t>ThermoPol</t> buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.
    T7 Rna Polymerase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t7 rna polymerase buffer/product/New England Biolabs
    Average 95 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    t7 rna polymerase buffer - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs mnase buffer
    Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× <t>ThermoPol</t> buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.
    Mnase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mnase buffer/product/New England Biolabs
    Average 95 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
    mnase buffer - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    80
    New England Biolabs thermolpol reaction buffer
    Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× <t>ThermoPol</t> buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.
    Thermolpol Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermolpol reaction buffer/product/New England Biolabs
    Average 80 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    thermolpol reaction buffer - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    95
    New England Biolabs t4 rna ligase buffer
    Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× <t>ThermoPol</t> buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.
    T4 Rna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 336 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase buffer/product/New England Biolabs
    Average 95 stars, based on 336 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase buffer - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    90
    New England Biolabs dnase buffer
    Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× <t>ThermoPol</t> buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.
    Dnase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase buffer/product/New England Biolabs
    Average 90 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    dnase buffer - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    New England Biolabs rnase one buffer
    Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× <t>ThermoPol</t> buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.
    Rnase One Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase one buffer/product/New England Biolabs
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rnase one buffer - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    95
    New England Biolabs λ dna
    Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× <t>ThermoPol</t> buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.
    λ Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 542 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/λ dna/product/New England Biolabs
    Average 95 stars, based on 542 article reviews
    Price from $9.99 to $1999.99
    λ dna - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs taq dna ligase buffer
    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic <t>DNA.</t> SNP allele discrimination is based on the specificity of the <t>Taq</t> ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only
    Taq Dna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna ligase buffer/product/New England Biolabs
    Average 95 stars, based on 159 article reviews
    Price from $9.99 to $1999.99
    taq dna ligase buffer - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs 1x isothermal buffer
    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic <t>DNA.</t> SNP allele discrimination is based on the specificity of the <t>Taq</t> ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only
    1x Isothermal Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x isothermal buffer/product/New England Biolabs
    Average 95 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    1x isothermal buffer - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    99
    New England Biolabs buffer 3
    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic <t>DNA.</t> SNP allele discrimination is based on the specificity of the <t>Taq</t> ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only
    Buffer 3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 238 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffer 3/product/New England Biolabs
    Average 99 stars, based on 238 article reviews
    Price from $9.99 to $1999.99
    buffer 3 - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    95
    New England Biolabs bp dna ladder
    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic <t>DNA.</t> SNP allele discrimination is based on the specificity of the <t>Taq</t> ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only
    Bp Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bp dna ladder/product/New England Biolabs
    Average 95 stars, based on 578 article reviews
    Price from $9.99 to $1999.99
    bp dna ladder - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    79
    New England Biolabs aspn reaction buffer
    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic <t>DNA.</t> SNP allele discrimination is based on the specificity of the <t>Taq</t> ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only
    Aspn Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspn reaction buffer/product/New England Biolabs
    Average 79 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    aspn reaction buffer - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    81
    New England Biolabs 10x buffer 3
    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic <t>DNA.</t> SNP allele discrimination is based on the specificity of the <t>Taq</t> ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only
    10x Buffer 3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 81/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x buffer 3/product/New England Biolabs
    Average 81 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    10x buffer 3 - by Bioz Stars, 2020-02
    81/100 stars
      Buy from Supplier

    95
    New England Biolabs m mulv reverse transcriptase reaction buffer
    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic <t>DNA.</t> SNP allele discrimination is based on the specificity of the <t>Taq</t> ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only
    M Mulv Reverse Transcriptase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mulv reverse transcriptase reaction buffer/product/New England Biolabs
    Average 95 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    m mulv reverse transcriptase reaction buffer - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs t4 polynucleotide kinase
    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic <t>DNA.</t> SNP allele discrimination is based on the specificity of the <t>Taq</t> ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only
    T4 Polynucleotide Kinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 24211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 polynucleotide kinase/product/New England Biolabs
    Average 95 stars, based on 24211 article reviews
    Price from $9.99 to $1999.99
    t4 polynucleotide kinase - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs isothermal amplification buffer
    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic <t>DNA.</t> SNP allele discrimination is based on the specificity of the <t>Taq</t> ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only
    Isothermal Amplification Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 216 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isothermal amplification buffer/product/New England Biolabs
    Average 95 stars, based on 216 article reviews
    Price from $9.99 to $1999.99
    isothermal amplification buffer - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    79
    New England Biolabs rnapoly reaction buffer
    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic <t>DNA.</t> SNP allele discrimination is based on the specificity of the <t>Taq</t> ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only
    Rnapoly Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnapoly reaction buffer/product/New England Biolabs
    Average 79 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    rnapoly reaction buffer - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    91
    New England Biolabs 1x rnapol reaction buffer
    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic <t>DNA.</t> SNP allele discrimination is based on the specificity of the <t>Taq</t> ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only
    1x Rnapol Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x rnapol reaction buffer/product/New England Biolabs
    Average 91 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    1x rnapol reaction buffer - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

    92
    New England Biolabs nucleus extraction buffer
    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic <t>DNA.</t> SNP allele discrimination is based on the specificity of the <t>Taq</t> ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only
    Nucleus Extraction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nucleus extraction buffer/product/New England Biolabs
    Average 92 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    nucleus extraction buffer - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

    99
    New England Biolabs 1x thermopol buffer
    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic <t>DNA.</t> SNP allele discrimination is based on the specificity of the <t>Taq</t> ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only
    1x Thermopol Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x thermopol buffer/product/New England Biolabs
    Average 99 stars, based on 157 article reviews
    Price from $9.99 to $1999.99
    1x thermopol buffer - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    95
    New England Biolabs t4 pnk
    Purification and activity assays of PNKP and Polβ. ( A ) Purified Polβ-His 6 (17 ng) and PNKP-His 6 (127 ng) from E. coli were subjected to PAGE and stained with Coomassie blue. ( B ) Incorporation of [α- 32 P]-dCTP by Polβ using APE1-generated product. ddC, di-deoxynucleotide; P, product. ( C ) Schematic of the preparation of S (substrate) and subsequent enzymatic reactions for testing PNKP activity. ( D ) Efficiency of oligonucleotide labeling, annealing, and ligation leading to S indicated in ( C ). ( E ) Fpg (NEB, 1 U) completely digested S and the 3’ phosphate was completely removed by PNKP (12.7 ng and 127 ng, lanes 2 and 3), or by <t>T4</t> PNK (NEB, 0.1 U and 1 U, lanes 7 and 8). NEIL2 (272 ng) only partially digested S and its 3’P was resistant to the PNKP phosphatase (lanes 4 and 5).
    T4 Pnk, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 3163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 pnk/product/New England Biolabs
    Average 95 stars, based on 3163 article reviews
    Price from $9.99 to $1999.99
    t4 pnk - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    91
    New England Biolabs hmt reaction buffer
    Purification and activity assays of PNKP and Polβ. ( A ) Purified Polβ-His 6 (17 ng) and PNKP-His 6 (127 ng) from E. coli were subjected to PAGE and stained with Coomassie blue. ( B ) Incorporation of [α- 32 P]-dCTP by Polβ using APE1-generated product. ddC, di-deoxynucleotide; P, product. ( C ) Schematic of the preparation of S (substrate) and subsequent enzymatic reactions for testing PNKP activity. ( D ) Efficiency of oligonucleotide labeling, annealing, and ligation leading to S indicated in ( C ). ( E ) Fpg (NEB, 1 U) completely digested S and the 3’ phosphate was completely removed by PNKP (12.7 ng and 127 ng, lanes 2 and 3), or by <t>T4</t> PNK (NEB, 0.1 U and 1 U, lanes 7 and 8). NEIL2 (272 ng) only partially digested S and its 3’P was resistant to the PNKP phosphatase (lanes 4 and 5).
    Hmt Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hmt reaction buffer/product/New England Biolabs
    Average 91 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    hmt reaction buffer - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

    Image Search Results


    Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× ThermoPol buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.

    Journal: Nucleic Acids Research

    Article Title: Improved nucleotide selectivity and termination of 3?-OH unblocked reversible terminators by molecular tuning of 2-nitrobenzyl alkylated HOMedU triphosphates

    doi: 10.1093/nar/gkq1293

    Figure Lengend Snippet: Ten-base CRT sequencing with dU.V . ( A ) 377 DNA sequencer gel image of a 10-cycle CRT experiment. Therminator polymerase was bound to primer/template complex and subjected to multiple cycles of incorporation (5 min) with 3 μM dU.V in 1× ThermoPol buffer, plus UV cleavage (4 min) in 50 mM sodium azide. P, dye-labeled primer (blue star represents BODIPY-FL label); 1a, first incorporation; 1b, first cleavage; 2a–10a, subsequent cleavage and incorporation cycles on dye-labeled primer. ( B ) Histogram plot of quantified gel bands in (A). Cycle efficiency was determined as a product of incorporation efficiency (1a: 100%) and cleavage efficiency (1b: 100%). Dephasing signals were also quantified as ‘% n –2’ (incomplete incorporation for the current cycle), ‘% n –1’ (incomplete UV cleavage from the previous cycle, extension of the ‘% n –2’ from the previous cycle and/or 3′-exonuclease activity followed by incorporation in the current cycle) and ‘% n +1’ [natural (photochemically cleaved) nucleotide carryover from the previous cycle]. ( C ) Histogram plot including the total signal for each cycle, representing the sum of the correct signal plus all dephasing signals.

    Article Snippet: Approximately 50 ng of genomic DNA was amplified with 0.4 µM of HNF1a_2.1 (F/R) or HNF1a_10.1 (F/R) primer pairs and one unit of Vent(exo− ) polymerase in 1× ThermoPol buffer [20 mM Tris–HCl, pH 8.8; 10 mM (NH4 )2 SO4 ; 10 mM KCl; 2 mM MgSO4 ; 0.1% Triton X-100; New England BioLabs], 1 M betaine ( , ) and 200 μM each of dATP, dCTP, dGTP and either TTP or HOMedUTP.

    Techniques: Sequencing, Labeling, Activity Assay

    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic DNA. SNP allele discrimination is based on the specificity of the Taq ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only

    Journal: Nucleic Acids Research

    Article Title: SNPWaveTM: a flexible multiplexed SNP genotyping technology

    doi: 10.1093/nar/gnh045

    Figure Lengend Snippet: Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic DNA. SNP allele discrimination is based on the specificity of the Taq ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only

    Article Snippet: Ligation reactions for Arabidopsis polymorphisms were performed in a 25 µl volume containing 625 ng of Arabidopsis DNA, 1× Taq DNA ligase buffer [20 mM Tris–HCl, 25 mM KAc, 10 mM MgAc2 , 10 mM dithiothreitol (DTT), 1 mM NAD, 0.1% Triton X-100; pH 7.6 at 25°C; New England Biolabs Inc., Beverly, MA], 0.2 U/µl Taq DNA ligase (NEB) and 0.05 fmol/µl of each of 200 ligation probes.

    Techniques: Ligation

    Purification and activity assays of PNKP and Polβ. ( A ) Purified Polβ-His 6 (17 ng) and PNKP-His 6 (127 ng) from E. coli were subjected to PAGE and stained with Coomassie blue. ( B ) Incorporation of [α- 32 P]-dCTP by Polβ using APE1-generated product. ddC, di-deoxynucleotide; P, product. ( C ) Schematic of the preparation of S (substrate) and subsequent enzymatic reactions for testing PNKP activity. ( D ) Efficiency of oligonucleotide labeling, annealing, and ligation leading to S indicated in ( C ). ( E ) Fpg (NEB, 1 U) completely digested S and the 3’ phosphate was completely removed by PNKP (12.7 ng and 127 ng, lanes 2 and 3), or by T4 PNK (NEB, 0.1 U and 1 U, lanes 7 and 8). NEIL2 (272 ng) only partially digested S and its 3’P was resistant to the PNKP phosphatase (lanes 4 and 5).

    Journal: eLife

    Article Title: Perturbation of base excision repair sensitizes breast cancer cells to APOBEC3 deaminase-mediated mutations

    doi: 10.7554/eLife.51605

    Figure Lengend Snippet: Purification and activity assays of PNKP and Polβ. ( A ) Purified Polβ-His 6 (17 ng) and PNKP-His 6 (127 ng) from E. coli were subjected to PAGE and stained with Coomassie blue. ( B ) Incorporation of [α- 32 P]-dCTP by Polβ using APE1-generated product. ddC, di-deoxynucleotide; P, product. ( C ) Schematic of the preparation of S (substrate) and subsequent enzymatic reactions for testing PNKP activity. ( D ) Efficiency of oligonucleotide labeling, annealing, and ligation leading to S indicated in ( C ). ( E ) Fpg (NEB, 1 U) completely digested S and the 3’ phosphate was completely removed by PNKP (12.7 ng and 127 ng, lanes 2 and 3), or by T4 PNK (NEB, 0.1 U and 1 U, lanes 7 and 8). NEIL2 (272 ng) only partially digested S and its 3’P was resistant to the PNKP phosphatase (lanes 4 and 5).

    Article Snippet: The products were purified by PCI extraction and ethanol precipitation and treated with PNKP (25 mM HEPES-KOH, pH 7.6, 50 mM NaCl, 0.5 mM EDTA, 0.5 mM DTT, 100 ng/ml BSA, 5% Glycerol) or T4 PNK (70 mM Tris-HCl, 10 mM MgCl2 , 5 mM DTT, pH 6.0) at 37°C for 30 min.

    Techniques: Purification, Activity Assay, Polyacrylamide Gel Electrophoresis, Staining, Generated, Oligonucleotide Labeling, Ligation