tris-buffered saline Search Results


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  • 99
    Thermo Fisher tbst
    Tbst, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 37653 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore tris buffered saline
    Tris Buffered Saline, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 18302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc tris buffered saline
    Tris Buffered Saline, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 10124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology tris buffered saline
    Tris Buffered Saline, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 12205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam tris buffered saline
    Tris Buffered Saline, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 10637 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tris buffered saline
    Tris Buffered Saline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 7131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore tris buffered saline tbs
    Tris Buffered Saline Tbs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa tb green premix ex taq ii
    Tb Green Premix Ex Taq Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1710 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad tris buffered saline
    Tris Buffered Saline, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 4135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tris buffered saline tbs
    Tris Buffered Saline Tbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1825 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies tris buffered saline
    Targeted fib-GC-AuNPs are superior to non-targeted GC-AuNPs in fibrin-binding and mCT imaging of cerebral thromboemboli. A , Schematic diagram of fibrin-targeted gold nanoparticles. B , In vitro experiments to show a higher fibrin-binding capacity of fib-GC-AuNPs vs. GC-AuNPs. When either fibrinogen or thrombin was added to GC-AuNP colloid (upper row) or fib-GC-AuNP colloid (middle row), the red coloring caused by dissolute colloids did not change. In contrast, when either preformed fibrin clot was immersed in the fib-GC-AuNP colloid (upper row) or if fibrin clot was formed in situ in the fib-GC-AuNP colloid (middle row), the redness (upper and middle rows) and UV absorbance (lower row) caused by the nanoparticle colloids was decreased and the clots formed were stained a red color. These changes suggest that both non-targeted GC-AuNPs and fibrin-targeted fib-GC-AuNPs have bound to the clot, reducing the amount of colloid in free solution. Considering the relatively weak changes in the color and UV absorbance of the non-targeted GC-AuNP colloid as well as in the color of the preformed and in situ clots, the fibrin-binding capacity of GC-AuNPs is substantially lower than that of fib-GC-AuNPs. <t>TBS</t> denotes <t>Tris-buffered</t> saline. C and D , representative mCT thrombus images / Cy5.5 near-infrared fluorescent (NIRF) thrombus images of C57Bl/6 mice with embolic stroke ( C ) and grouped quantification data for the areas of embolic clots in the black and red dotted squares ( D ). Embolic stroke was induced by injecting Cy5.5-fluorescently labeled clot into the bifurcation area of the left distal internal carotid artery of mice (n = 56). One hour later, mCT thrombus images (upper row in C ) were acquired 5 minutes after intravenous injection of 300 μL GC-AuNPs or fib-GC-AuNPs of either a low (12 mg/kg; n = 5 / group) or high concentration (120 mg/kg; n = 23 / group). After each animal was sacrificed and the brain was collected, ex vivo optical imaging was performed to obtain a Cy5.5 NIRF thrombus image (lower row in C ), which served as a exogenously labeled standard for the purposes of comparing to the corresponding mCT image. At both the high and low concentrations, targeted fib-GC-AuNPs visualize the Y-shape cerebral thrombi better than non-targeted GC-AuNPs (black dotted squares in C ). Note how the fluorescent embolic thrombus (red dotted squares in C ) is similar for all animals, but how the same thrombi are much better visualized with targeted vs. non-targeted nanoparticles by CT (black dotted squares in C ). Regardless of the type of imaging agent, the high concentration is superior to the low concentration in the CT visualization of cerebral thrombi. The above imaging findings from the representative animals ( C ) are corroborated by the quantification data for all 56 animals ( D ); the visualized thrombus area ratio (mCT/NIRF) is the highest in the high concentration fib-GC-AuNP group, followed by the high concentration GC-AuNP group, the low concentration fib-GC-AuNP group, and last the lowest in the low concentration GC-AuNP group. E and F , mCT visualization ( E ) and quantification ( F ) of cerebral thromboemboli after sequential administrations (blue colored +→) of first non-targeted GC-AuNP and then targeted fib-GC-AuNP, and vice versa. After intravenous (i.v.) injection of GC-AuNPs (120 mg/kg, 300 μL) 50 minutes after embolic stroke, there is very weak parenchymal mCT hyperdensity in the circle of Willis (black dotted square in the 1 st column of the upper row). A subsequent mCT image at 60 minutes, obtained after administering fib-GC-AuNPs (120 mg/kg, 300 μL), clearly visualizes cerebral thrombus (black dotted square in the 2 nd column of the upper row), which co-localizes to thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the second column of the lower row). When the order of injecting the two types of imaging agent was reversed, mCT visualization of cerebral thrombus by using fib-GC-AuNPs is not further improved by additionally using GC-AuNPs (black dotted squares in the 3 rd and 4 th columns of the upper row), despite the presence of thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the 4 th column of the lower row). These findings from the representative animals are corroborated by quantified data (the areas of embolic clots in the black and red dotted squares) for all 14 animals ( F ; n = 7 / group). Graphs show mean ± SEM. * P
    Tris Buffered Saline, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 3306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa tb green premix ex taq
    Targeted fib-GC-AuNPs are superior to non-targeted GC-AuNPs in fibrin-binding and mCT imaging of cerebral thromboemboli. A , Schematic diagram of fibrin-targeted gold nanoparticles. B , In vitro experiments to show a higher fibrin-binding capacity of fib-GC-AuNPs vs. GC-AuNPs. When either fibrinogen or thrombin was added to GC-AuNP colloid (upper row) or fib-GC-AuNP colloid (middle row), the red coloring caused by dissolute colloids did not change. In contrast, when either preformed fibrin clot was immersed in the fib-GC-AuNP colloid (upper row) or if fibrin clot was formed in situ in the fib-GC-AuNP colloid (middle row), the redness (upper and middle rows) and UV absorbance (lower row) caused by the nanoparticle colloids was decreased and the clots formed were stained a red color. These changes suggest that both non-targeted GC-AuNPs and fibrin-targeted fib-GC-AuNPs have bound to the clot, reducing the amount of colloid in free solution. Considering the relatively weak changes in the color and UV absorbance of the non-targeted GC-AuNP colloid as well as in the color of the preformed and in situ clots, the fibrin-binding capacity of GC-AuNPs is substantially lower than that of fib-GC-AuNPs. <t>TBS</t> denotes <t>Tris-buffered</t> saline. C and D , representative mCT thrombus images / Cy5.5 near-infrared fluorescent (NIRF) thrombus images of C57Bl/6 mice with embolic stroke ( C ) and grouped quantification data for the areas of embolic clots in the black and red dotted squares ( D ). Embolic stroke was induced by injecting Cy5.5-fluorescently labeled clot into the bifurcation area of the left distal internal carotid artery of mice (n = 56). One hour later, mCT thrombus images (upper row in C ) were acquired 5 minutes after intravenous injection of 300 μL GC-AuNPs or fib-GC-AuNPs of either a low (12 mg/kg; n = 5 / group) or high concentration (120 mg/kg; n = 23 / group). After each animal was sacrificed and the brain was collected, ex vivo optical imaging was performed to obtain a Cy5.5 NIRF thrombus image (lower row in C ), which served as a exogenously labeled standard for the purposes of comparing to the corresponding mCT image. At both the high and low concentrations, targeted fib-GC-AuNPs visualize the Y-shape cerebral thrombi better than non-targeted GC-AuNPs (black dotted squares in C ). Note how the fluorescent embolic thrombus (red dotted squares in C ) is similar for all animals, but how the same thrombi are much better visualized with targeted vs. non-targeted nanoparticles by CT (black dotted squares in C ). Regardless of the type of imaging agent, the high concentration is superior to the low concentration in the CT visualization of cerebral thrombi. The above imaging findings from the representative animals ( C ) are corroborated by the quantification data for all 56 animals ( D ); the visualized thrombus area ratio (mCT/NIRF) is the highest in the high concentration fib-GC-AuNP group, followed by the high concentration GC-AuNP group, the low concentration fib-GC-AuNP group, and last the lowest in the low concentration GC-AuNP group. E and F , mCT visualization ( E ) and quantification ( F ) of cerebral thromboemboli after sequential administrations (blue colored +→) of first non-targeted GC-AuNP and then targeted fib-GC-AuNP, and vice versa. After intravenous (i.v.) injection of GC-AuNPs (120 mg/kg, 300 μL) 50 minutes after embolic stroke, there is very weak parenchymal mCT hyperdensity in the circle of Willis (black dotted square in the 1 st column of the upper row). A subsequent mCT image at 60 minutes, obtained after administering fib-GC-AuNPs (120 mg/kg, 300 μL), clearly visualizes cerebral thrombus (black dotted square in the 2 nd column of the upper row), which co-localizes to thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the second column of the lower row). When the order of injecting the two types of imaging agent was reversed, mCT visualization of cerebral thrombus by using fib-GC-AuNPs is not further improved by additionally using GC-AuNPs (black dotted squares in the 3 rd and 4 th columns of the upper row), despite the presence of thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the 4 th column of the lower row). These findings from the representative animals are corroborated by quantified data (the areas of embolic clots in the black and red dotted squares) for all 14 animals ( F ; n = 7 / group). Graphs show mean ± SEM. * P
    Tb Green Premix Ex Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4358 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam tween 20 tbs t
    Targeted fib-GC-AuNPs are superior to non-targeted GC-AuNPs in fibrin-binding and mCT imaging of cerebral thromboemboli. A , Schematic diagram of fibrin-targeted gold nanoparticles. B , In vitro experiments to show a higher fibrin-binding capacity of fib-GC-AuNPs vs. GC-AuNPs. When either fibrinogen or thrombin was added to GC-AuNP colloid (upper row) or fib-GC-AuNP colloid (middle row), the red coloring caused by dissolute colloids did not change. In contrast, when either preformed fibrin clot was immersed in the fib-GC-AuNP colloid (upper row) or if fibrin clot was formed in situ in the fib-GC-AuNP colloid (middle row), the redness (upper and middle rows) and UV absorbance (lower row) caused by the nanoparticle colloids was decreased and the clots formed were stained a red color. These changes suggest that both non-targeted GC-AuNPs and fibrin-targeted fib-GC-AuNPs have bound to the clot, reducing the amount of colloid in free solution. Considering the relatively weak changes in the color and UV absorbance of the non-targeted GC-AuNP colloid as well as in the color of the preformed and in situ clots, the fibrin-binding capacity of GC-AuNPs is substantially lower than that of fib-GC-AuNPs. <t>TBS</t> denotes <t>Tris-buffered</t> saline. C and D , representative mCT thrombus images / Cy5.5 near-infrared fluorescent (NIRF) thrombus images of C57Bl/6 mice with embolic stroke ( C ) and grouped quantification data for the areas of embolic clots in the black and red dotted squares ( D ). Embolic stroke was induced by injecting Cy5.5-fluorescently labeled clot into the bifurcation area of the left distal internal carotid artery of mice (n = 56). One hour later, mCT thrombus images (upper row in C ) were acquired 5 minutes after intravenous injection of 300 μL GC-AuNPs or fib-GC-AuNPs of either a low (12 mg/kg; n = 5 / group) or high concentration (120 mg/kg; n = 23 / group). After each animal was sacrificed and the brain was collected, ex vivo optical imaging was performed to obtain a Cy5.5 NIRF thrombus image (lower row in C ), which served as a exogenously labeled standard for the purposes of comparing to the corresponding mCT image. At both the high and low concentrations, targeted fib-GC-AuNPs visualize the Y-shape cerebral thrombi better than non-targeted GC-AuNPs (black dotted squares in C ). Note how the fluorescent embolic thrombus (red dotted squares in C ) is similar for all animals, but how the same thrombi are much better visualized with targeted vs. non-targeted nanoparticles by CT (black dotted squares in C ). Regardless of the type of imaging agent, the high concentration is superior to the low concentration in the CT visualization of cerebral thrombi. The above imaging findings from the representative animals ( C ) are corroborated by the quantification data for all 56 animals ( D ); the visualized thrombus area ratio (mCT/NIRF) is the highest in the high concentration fib-GC-AuNP group, followed by the high concentration GC-AuNP group, the low concentration fib-GC-AuNP group, and last the lowest in the low concentration GC-AuNP group. E and F , mCT visualization ( E ) and quantification ( F ) of cerebral thromboemboli after sequential administrations (blue colored +→) of first non-targeted GC-AuNP and then targeted fib-GC-AuNP, and vice versa. After intravenous (i.v.) injection of GC-AuNPs (120 mg/kg, 300 μL) 50 minutes after embolic stroke, there is very weak parenchymal mCT hyperdensity in the circle of Willis (black dotted square in the 1 st column of the upper row). A subsequent mCT image at 60 minutes, obtained after administering fib-GC-AuNPs (120 mg/kg, 300 μL), clearly visualizes cerebral thrombus (black dotted square in the 2 nd column of the upper row), which co-localizes to thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the second column of the lower row). When the order of injecting the two types of imaging agent was reversed, mCT visualization of cerebral thrombus by using fib-GC-AuNPs is not further improved by additionally using GC-AuNPs (black dotted squares in the 3 rd and 4 th columns of the upper row), despite the presence of thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the 4 th column of the lower row). These findings from the representative animals are corroborated by quantified data (the areas of embolic clots in the black and red dotted squares) for all 14 animals ( F ; n = 7 / group). Graphs show mean ± SEM. * P
    Tween 20 Tbs T, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1539 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beyotime tris buffered saline
    Targeted fib-GC-AuNPs are superior to non-targeted GC-AuNPs in fibrin-binding and mCT imaging of cerebral thromboemboli. A , Schematic diagram of fibrin-targeted gold nanoparticles. B , In vitro experiments to show a higher fibrin-binding capacity of fib-GC-AuNPs vs. GC-AuNPs. When either fibrinogen or thrombin was added to GC-AuNP colloid (upper row) or fib-GC-AuNP colloid (middle row), the red coloring caused by dissolute colloids did not change. In contrast, when either preformed fibrin clot was immersed in the fib-GC-AuNP colloid (upper row) or if fibrin clot was formed in situ in the fib-GC-AuNP colloid (middle row), the redness (upper and middle rows) and UV absorbance (lower row) caused by the nanoparticle colloids was decreased and the clots formed were stained a red color. These changes suggest that both non-targeted GC-AuNPs and fibrin-targeted fib-GC-AuNPs have bound to the clot, reducing the amount of colloid in free solution. Considering the relatively weak changes in the color and UV absorbance of the non-targeted GC-AuNP colloid as well as in the color of the preformed and in situ clots, the fibrin-binding capacity of GC-AuNPs is substantially lower than that of fib-GC-AuNPs. <t>TBS</t> denotes <t>Tris-buffered</t> saline. C and D , representative mCT thrombus images / Cy5.5 near-infrared fluorescent (NIRF) thrombus images of C57Bl/6 mice with embolic stroke ( C ) and grouped quantification data for the areas of embolic clots in the black and red dotted squares ( D ). Embolic stroke was induced by injecting Cy5.5-fluorescently labeled clot into the bifurcation area of the left distal internal carotid artery of mice (n = 56). One hour later, mCT thrombus images (upper row in C ) were acquired 5 minutes after intravenous injection of 300 μL GC-AuNPs or fib-GC-AuNPs of either a low (12 mg/kg; n = 5 / group) or high concentration (120 mg/kg; n = 23 / group). After each animal was sacrificed and the brain was collected, ex vivo optical imaging was performed to obtain a Cy5.5 NIRF thrombus image (lower row in C ), which served as a exogenously labeled standard for the purposes of comparing to the corresponding mCT image. At both the high and low concentrations, targeted fib-GC-AuNPs visualize the Y-shape cerebral thrombi better than non-targeted GC-AuNPs (black dotted squares in C ). Note how the fluorescent embolic thrombus (red dotted squares in C ) is similar for all animals, but how the same thrombi are much better visualized with targeted vs. non-targeted nanoparticles by CT (black dotted squares in C ). Regardless of the type of imaging agent, the high concentration is superior to the low concentration in the CT visualization of cerebral thrombi. The above imaging findings from the representative animals ( C ) are corroborated by the quantification data for all 56 animals ( D ); the visualized thrombus area ratio (mCT/NIRF) is the highest in the high concentration fib-GC-AuNP group, followed by the high concentration GC-AuNP group, the low concentration fib-GC-AuNP group, and last the lowest in the low concentration GC-AuNP group. E and F , mCT visualization ( E ) and quantification ( F ) of cerebral thromboemboli after sequential administrations (blue colored +→) of first non-targeted GC-AuNP and then targeted fib-GC-AuNP, and vice versa. After intravenous (i.v.) injection of GC-AuNPs (120 mg/kg, 300 μL) 50 minutes after embolic stroke, there is very weak parenchymal mCT hyperdensity in the circle of Willis (black dotted square in the 1 st column of the upper row). A subsequent mCT image at 60 minutes, obtained after administering fib-GC-AuNPs (120 mg/kg, 300 μL), clearly visualizes cerebral thrombus (black dotted square in the 2 nd column of the upper row), which co-localizes to thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the second column of the lower row). When the order of injecting the two types of imaging agent was reversed, mCT visualization of cerebral thrombus by using fib-GC-AuNPs is not further improved by additionally using GC-AuNPs (black dotted squares in the 3 rd and 4 th columns of the upper row), despite the presence of thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the 4 th column of the lower row). These findings from the representative animals are corroborated by quantified data (the areas of embolic clots in the black and red dotted squares) for all 14 animals ( F ; n = 7 / group). Graphs show mean ± SEM. * P
    Tris Buffered Saline, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pierce 20x tbs tween 20 buffer
    Targeted fib-GC-AuNPs are superior to non-targeted GC-AuNPs in fibrin-binding and mCT imaging of cerebral thromboemboli. A , Schematic diagram of fibrin-targeted gold nanoparticles. B , In vitro experiments to show a higher fibrin-binding capacity of fib-GC-AuNPs vs. GC-AuNPs. When either fibrinogen or thrombin was added to GC-AuNP colloid (upper row) or fib-GC-AuNP colloid (middle row), the red coloring caused by dissolute colloids did not change. In contrast, when either preformed fibrin clot was immersed in the fib-GC-AuNP colloid (upper row) or if fibrin clot was formed in situ in the fib-GC-AuNP colloid (middle row), the redness (upper and middle rows) and UV absorbance (lower row) caused by the nanoparticle colloids was decreased and the clots formed were stained a red color. These changes suggest that both non-targeted GC-AuNPs and fibrin-targeted fib-GC-AuNPs have bound to the clot, reducing the amount of colloid in free solution. Considering the relatively weak changes in the color and UV absorbance of the non-targeted GC-AuNP colloid as well as in the color of the preformed and in situ clots, the fibrin-binding capacity of GC-AuNPs is substantially lower than that of fib-GC-AuNPs. <t>TBS</t> denotes <t>Tris-buffered</t> saline. C and D , representative mCT thrombus images / Cy5.5 near-infrared fluorescent (NIRF) thrombus images of C57Bl/6 mice with embolic stroke ( C ) and grouped quantification data for the areas of embolic clots in the black and red dotted squares ( D ). Embolic stroke was induced by injecting Cy5.5-fluorescently labeled clot into the bifurcation area of the left distal internal carotid artery of mice (n = 56). One hour later, mCT thrombus images (upper row in C ) were acquired 5 minutes after intravenous injection of 300 μL GC-AuNPs or fib-GC-AuNPs of either a low (12 mg/kg; n = 5 / group) or high concentration (120 mg/kg; n = 23 / group). After each animal was sacrificed and the brain was collected, ex vivo optical imaging was performed to obtain a Cy5.5 NIRF thrombus image (lower row in C ), which served as a exogenously labeled standard for the purposes of comparing to the corresponding mCT image. At both the high and low concentrations, targeted fib-GC-AuNPs visualize the Y-shape cerebral thrombi better than non-targeted GC-AuNPs (black dotted squares in C ). Note how the fluorescent embolic thrombus (red dotted squares in C ) is similar for all animals, but how the same thrombi are much better visualized with targeted vs. non-targeted nanoparticles by CT (black dotted squares in C ). Regardless of the type of imaging agent, the high concentration is superior to the low concentration in the CT visualization of cerebral thrombi. The above imaging findings from the representative animals ( C ) are corroborated by the quantification data for all 56 animals ( D ); the visualized thrombus area ratio (mCT/NIRF) is the highest in the high concentration fib-GC-AuNP group, followed by the high concentration GC-AuNP group, the low concentration fib-GC-AuNP group, and last the lowest in the low concentration GC-AuNP group. E and F , mCT visualization ( E ) and quantification ( F ) of cerebral thromboemboli after sequential administrations (blue colored +→) of first non-targeted GC-AuNP and then targeted fib-GC-AuNP, and vice versa. After intravenous (i.v.) injection of GC-AuNPs (120 mg/kg, 300 μL) 50 minutes after embolic stroke, there is very weak parenchymal mCT hyperdensity in the circle of Willis (black dotted square in the 1 st column of the upper row). A subsequent mCT image at 60 minutes, obtained after administering fib-GC-AuNPs (120 mg/kg, 300 μL), clearly visualizes cerebral thrombus (black dotted square in the 2 nd column of the upper row), which co-localizes to thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the second column of the lower row). When the order of injecting the two types of imaging agent was reversed, mCT visualization of cerebral thrombus by using fib-GC-AuNPs is not further improved by additionally using GC-AuNPs (black dotted squares in the 3 rd and 4 th columns of the upper row), despite the presence of thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the 4 th column of the lower row). These findings from the representative animals are corroborated by quantified data (the areas of embolic clots in the black and red dotted squares) for all 14 animals ( F ; n = 7 / group). Graphs show mean ± SEM. * P
    Pierce 20x Tbs Tween 20 Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad tris buffered saline tbs
    Targeted fib-GC-AuNPs are superior to non-targeted GC-AuNPs in fibrin-binding and mCT imaging of cerebral thromboemboli. A , Schematic diagram of fibrin-targeted gold nanoparticles. B , In vitro experiments to show a higher fibrin-binding capacity of fib-GC-AuNPs vs. GC-AuNPs. When either fibrinogen or thrombin was added to GC-AuNP colloid (upper row) or fib-GC-AuNP colloid (middle row), the red coloring caused by dissolute colloids did not change. In contrast, when either preformed fibrin clot was immersed in the fib-GC-AuNP colloid (upper row) or if fibrin clot was formed in situ in the fib-GC-AuNP colloid (middle row), the redness (upper and middle rows) and UV absorbance (lower row) caused by the nanoparticle colloids was decreased and the clots formed were stained a red color. These changes suggest that both non-targeted GC-AuNPs and fibrin-targeted fib-GC-AuNPs have bound to the clot, reducing the amount of colloid in free solution. Considering the relatively weak changes in the color and UV absorbance of the non-targeted GC-AuNP colloid as well as in the color of the preformed and in situ clots, the fibrin-binding capacity of GC-AuNPs is substantially lower than that of fib-GC-AuNPs. <t>TBS</t> denotes <t>Tris-buffered</t> saline. C and D , representative mCT thrombus images / Cy5.5 near-infrared fluorescent (NIRF) thrombus images of C57Bl/6 mice with embolic stroke ( C ) and grouped quantification data for the areas of embolic clots in the black and red dotted squares ( D ). Embolic stroke was induced by injecting Cy5.5-fluorescently labeled clot into the bifurcation area of the left distal internal carotid artery of mice (n = 56). One hour later, mCT thrombus images (upper row in C ) were acquired 5 minutes after intravenous injection of 300 μL GC-AuNPs or fib-GC-AuNPs of either a low (12 mg/kg; n = 5 / group) or high concentration (120 mg/kg; n = 23 / group). After each animal was sacrificed and the brain was collected, ex vivo optical imaging was performed to obtain a Cy5.5 NIRF thrombus image (lower row in C ), which served as a exogenously labeled standard for the purposes of comparing to the corresponding mCT image. At both the high and low concentrations, targeted fib-GC-AuNPs visualize the Y-shape cerebral thrombi better than non-targeted GC-AuNPs (black dotted squares in C ). Note how the fluorescent embolic thrombus (red dotted squares in C ) is similar for all animals, but how the same thrombi are much better visualized with targeted vs. non-targeted nanoparticles by CT (black dotted squares in C ). Regardless of the type of imaging agent, the high concentration is superior to the low concentration in the CT visualization of cerebral thrombi. The above imaging findings from the representative animals ( C ) are corroborated by the quantification data for all 56 animals ( D ); the visualized thrombus area ratio (mCT/NIRF) is the highest in the high concentration fib-GC-AuNP group, followed by the high concentration GC-AuNP group, the low concentration fib-GC-AuNP group, and last the lowest in the low concentration GC-AuNP group. E and F , mCT visualization ( E ) and quantification ( F ) of cerebral thromboemboli after sequential administrations (blue colored +→) of first non-targeted GC-AuNP and then targeted fib-GC-AuNP, and vice versa. After intravenous (i.v.) injection of GC-AuNPs (120 mg/kg, 300 μL) 50 minutes after embolic stroke, there is very weak parenchymal mCT hyperdensity in the circle of Willis (black dotted square in the 1 st column of the upper row). A subsequent mCT image at 60 minutes, obtained after administering fib-GC-AuNPs (120 mg/kg, 300 μL), clearly visualizes cerebral thrombus (black dotted square in the 2 nd column of the upper row), which co-localizes to thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the second column of the lower row). When the order of injecting the two types of imaging agent was reversed, mCT visualization of cerebral thrombus by using fib-GC-AuNPs is not further improved by additionally using GC-AuNPs (black dotted squares in the 3 rd and 4 th columns of the upper row), despite the presence of thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the 4 th column of the lower row). These findings from the representative animals are corroborated by quantified data (the areas of embolic clots in the black and red dotted squares) for all 14 animals ( F ; n = 7 / group). Graphs show mean ± SEM. * P
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    The relationship between IGRA response and TST induration. The distribution of positive IGRAs (pre- and post-TST) by size of TST induration. TST = tuberculin skin test; QFT-GIT = <t>QuantiFERON</t> Gold in-Tube.
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    Image Search Results


    Targeted fib-GC-AuNPs are superior to non-targeted GC-AuNPs in fibrin-binding and mCT imaging of cerebral thromboemboli. A , Schematic diagram of fibrin-targeted gold nanoparticles. B , In vitro experiments to show a higher fibrin-binding capacity of fib-GC-AuNPs vs. GC-AuNPs. When either fibrinogen or thrombin was added to GC-AuNP colloid (upper row) or fib-GC-AuNP colloid (middle row), the red coloring caused by dissolute colloids did not change. In contrast, when either preformed fibrin clot was immersed in the fib-GC-AuNP colloid (upper row) or if fibrin clot was formed in situ in the fib-GC-AuNP colloid (middle row), the redness (upper and middle rows) and UV absorbance (lower row) caused by the nanoparticle colloids was decreased and the clots formed were stained a red color. These changes suggest that both non-targeted GC-AuNPs and fibrin-targeted fib-GC-AuNPs have bound to the clot, reducing the amount of colloid in free solution. Considering the relatively weak changes in the color and UV absorbance of the non-targeted GC-AuNP colloid as well as in the color of the preformed and in situ clots, the fibrin-binding capacity of GC-AuNPs is substantially lower than that of fib-GC-AuNPs. TBS denotes Tris-buffered saline. C and D , representative mCT thrombus images / Cy5.5 near-infrared fluorescent (NIRF) thrombus images of C57Bl/6 mice with embolic stroke ( C ) and grouped quantification data for the areas of embolic clots in the black and red dotted squares ( D ). Embolic stroke was induced by injecting Cy5.5-fluorescently labeled clot into the bifurcation area of the left distal internal carotid artery of mice (n = 56). One hour later, mCT thrombus images (upper row in C ) were acquired 5 minutes after intravenous injection of 300 μL GC-AuNPs or fib-GC-AuNPs of either a low (12 mg/kg; n = 5 / group) or high concentration (120 mg/kg; n = 23 / group). After each animal was sacrificed and the brain was collected, ex vivo optical imaging was performed to obtain a Cy5.5 NIRF thrombus image (lower row in C ), which served as a exogenously labeled standard for the purposes of comparing to the corresponding mCT image. At both the high and low concentrations, targeted fib-GC-AuNPs visualize the Y-shape cerebral thrombi better than non-targeted GC-AuNPs (black dotted squares in C ). Note how the fluorescent embolic thrombus (red dotted squares in C ) is similar for all animals, but how the same thrombi are much better visualized with targeted vs. non-targeted nanoparticles by CT (black dotted squares in C ). Regardless of the type of imaging agent, the high concentration is superior to the low concentration in the CT visualization of cerebral thrombi. The above imaging findings from the representative animals ( C ) are corroborated by the quantification data for all 56 animals ( D ); the visualized thrombus area ratio (mCT/NIRF) is the highest in the high concentration fib-GC-AuNP group, followed by the high concentration GC-AuNP group, the low concentration fib-GC-AuNP group, and last the lowest in the low concentration GC-AuNP group. E and F , mCT visualization ( E ) and quantification ( F ) of cerebral thromboemboli after sequential administrations (blue colored +→) of first non-targeted GC-AuNP and then targeted fib-GC-AuNP, and vice versa. After intravenous (i.v.) injection of GC-AuNPs (120 mg/kg, 300 μL) 50 minutes after embolic stroke, there is very weak parenchymal mCT hyperdensity in the circle of Willis (black dotted square in the 1 st column of the upper row). A subsequent mCT image at 60 minutes, obtained after administering fib-GC-AuNPs (120 mg/kg, 300 μL), clearly visualizes cerebral thrombus (black dotted square in the 2 nd column of the upper row), which co-localizes to thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the second column of the lower row). When the order of injecting the two types of imaging agent was reversed, mCT visualization of cerebral thrombus by using fib-GC-AuNPs is not further improved by additionally using GC-AuNPs (black dotted squares in the 3 rd and 4 th columns of the upper row), despite the presence of thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the 4 th column of the lower row). These findings from the representative animals are corroborated by quantified data (the areas of embolic clots in the black and red dotted squares) for all 14 animals ( F ; n = 7 / group). Graphs show mean ± SEM. * P

    Journal: Theranostics

    Article Title: Direct Imaging of Cerebral Thromboemboli Using Computed Tomography and Fibrin-targeted Gold Nanoparticles

    doi: 10.7150/thno.11679

    Figure Lengend Snippet: Targeted fib-GC-AuNPs are superior to non-targeted GC-AuNPs in fibrin-binding and mCT imaging of cerebral thromboemboli. A , Schematic diagram of fibrin-targeted gold nanoparticles. B , In vitro experiments to show a higher fibrin-binding capacity of fib-GC-AuNPs vs. GC-AuNPs. When either fibrinogen or thrombin was added to GC-AuNP colloid (upper row) or fib-GC-AuNP colloid (middle row), the red coloring caused by dissolute colloids did not change. In contrast, when either preformed fibrin clot was immersed in the fib-GC-AuNP colloid (upper row) or if fibrin clot was formed in situ in the fib-GC-AuNP colloid (middle row), the redness (upper and middle rows) and UV absorbance (lower row) caused by the nanoparticle colloids was decreased and the clots formed were stained a red color. These changes suggest that both non-targeted GC-AuNPs and fibrin-targeted fib-GC-AuNPs have bound to the clot, reducing the amount of colloid in free solution. Considering the relatively weak changes in the color and UV absorbance of the non-targeted GC-AuNP colloid as well as in the color of the preformed and in situ clots, the fibrin-binding capacity of GC-AuNPs is substantially lower than that of fib-GC-AuNPs. TBS denotes Tris-buffered saline. C and D , representative mCT thrombus images / Cy5.5 near-infrared fluorescent (NIRF) thrombus images of C57Bl/6 mice with embolic stroke ( C ) and grouped quantification data for the areas of embolic clots in the black and red dotted squares ( D ). Embolic stroke was induced by injecting Cy5.5-fluorescently labeled clot into the bifurcation area of the left distal internal carotid artery of mice (n = 56). One hour later, mCT thrombus images (upper row in C ) were acquired 5 minutes after intravenous injection of 300 μL GC-AuNPs or fib-GC-AuNPs of either a low (12 mg/kg; n = 5 / group) or high concentration (120 mg/kg; n = 23 / group). After each animal was sacrificed and the brain was collected, ex vivo optical imaging was performed to obtain a Cy5.5 NIRF thrombus image (lower row in C ), which served as a exogenously labeled standard for the purposes of comparing to the corresponding mCT image. At both the high and low concentrations, targeted fib-GC-AuNPs visualize the Y-shape cerebral thrombi better than non-targeted GC-AuNPs (black dotted squares in C ). Note how the fluorescent embolic thrombus (red dotted squares in C ) is similar for all animals, but how the same thrombi are much better visualized with targeted vs. non-targeted nanoparticles by CT (black dotted squares in C ). Regardless of the type of imaging agent, the high concentration is superior to the low concentration in the CT visualization of cerebral thrombi. The above imaging findings from the representative animals ( C ) are corroborated by the quantification data for all 56 animals ( D ); the visualized thrombus area ratio (mCT/NIRF) is the highest in the high concentration fib-GC-AuNP group, followed by the high concentration GC-AuNP group, the low concentration fib-GC-AuNP group, and last the lowest in the low concentration GC-AuNP group. E and F , mCT visualization ( E ) and quantification ( F ) of cerebral thromboemboli after sequential administrations (blue colored +→) of first non-targeted GC-AuNP and then targeted fib-GC-AuNP, and vice versa. After intravenous (i.v.) injection of GC-AuNPs (120 mg/kg, 300 μL) 50 minutes after embolic stroke, there is very weak parenchymal mCT hyperdensity in the circle of Willis (black dotted square in the 1 st column of the upper row). A subsequent mCT image at 60 minutes, obtained after administering fib-GC-AuNPs (120 mg/kg, 300 μL), clearly visualizes cerebral thrombus (black dotted square in the 2 nd column of the upper row), which co-localizes to thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the second column of the lower row). When the order of injecting the two types of imaging agent was reversed, mCT visualization of cerebral thrombus by using fib-GC-AuNPs is not further improved by additionally using GC-AuNPs (black dotted squares in the 3 rd and 4 th columns of the upper row), despite the presence of thrombus-marking Cy5.5 signal on the ex vivo NIRF image (red dotted square in the 4 th column of the lower row). These findings from the representative animals are corroborated by quantified data (the areas of embolic clots in the black and red dotted squares) for all 14 animals ( F ; n = 7 / group). Graphs show mean ± SEM. * P

    Article Snippet: Fibrin polymerization was performed in Tris-buffered saline (TBS, pH 7.4; Dako, Glostrup, Denmark) containing 10 mmol/L of tris(hydroxymethyl) aminomethane.

    Techniques: Binding Assay, Imaging, In Vitro, In Situ, Staining, Mouse Assay, Labeling, Injection, Concentration Assay, Ex Vivo, Optical Imaging

    The relationship between IGRA response and TST induration. The distribution of positive IGRAs (pre- and post-TST) by size of TST induration. TST = tuberculin skin test; QFT-GIT = QuantiFERON Gold in-Tube.

    Journal: PLoS ONE

    Article Title: Tuberculin Skin Testing and Treatment Modulates Interferon-Gamma Release Assay Results for Latent Tuberculosis in Migrants

    doi: 10.1371/journal.pone.0097366

    Figure Lengend Snippet: The relationship between IGRA response and TST induration. The distribution of positive IGRAs (pre- and post-TST) by size of TST induration. TST = tuberculin skin test; QFT-GIT = QuantiFERON Gold in-Tube.

    Article Snippet: IGRA testing of recruits in the UK Two commercially available assays were used - The QuantiFERON-TB Gold In-Tube (QFT-GIT; Cellestis, Carnegie, Australia) whole blood enzyme-linked immunosorbent assay (ELISA), and the T-SPOT.TB assay (Oxford Immunotec, Abingdon, UK) enzyme-linked immunospot (ELISPOT) assay using peripheral blood mononuclear cells (PBMCs).

    Techniques:

    IGRA responses following LTBI treatment in individuals positive by IGRA at any stage of the study. (A) SFC numbers detected to ESAT-6 by T-SPOT.TB; (B) SFC numbers detected to CFP-10 by T-SPOT.TB; bars = medians; (C) IFN-γ responses detected by QFT-GIT in individuals who were initially positive at day 0 compared to day 200 (left) and IFN-γ responses detected by QFT-GIT in individuals who were positive at day 7 compared to day 200. In each case samples from the same individual at these two times are linked by bars. QFT-GIT = QuantiFERON Gold in-Tube; IFN-γ = interferon-gamma; ESAT-6 = early secretory antigenic target-6; CFP-10 = culture filtrate protein-10; IU = international units; SFC = spot forming cells; Rx = treatment.

    Journal: PLoS ONE

    Article Title: Tuberculin Skin Testing and Treatment Modulates Interferon-Gamma Release Assay Results for Latent Tuberculosis in Migrants

    doi: 10.1371/journal.pone.0097366

    Figure Lengend Snippet: IGRA responses following LTBI treatment in individuals positive by IGRA at any stage of the study. (A) SFC numbers detected to ESAT-6 by T-SPOT.TB; (B) SFC numbers detected to CFP-10 by T-SPOT.TB; bars = medians; (C) IFN-γ responses detected by QFT-GIT in individuals who were initially positive at day 0 compared to day 200 (left) and IFN-γ responses detected by QFT-GIT in individuals who were positive at day 7 compared to day 200. In each case samples from the same individual at these two times are linked by bars. QFT-GIT = QuantiFERON Gold in-Tube; IFN-γ = interferon-gamma; ESAT-6 = early secretory antigenic target-6; CFP-10 = culture filtrate protein-10; IU = international units; SFC = spot forming cells; Rx = treatment.

    Article Snippet: IGRA testing of recruits in the UK Two commercially available assays were used - The QuantiFERON-TB Gold In-Tube (QFT-GIT; Cellestis, Carnegie, Australia) whole blood enzyme-linked immunosorbent assay (ELISA), and the T-SPOT.TB assay (Oxford Immunotec, Abingdon, UK) enzyme-linked immunospot (ELISPOT) assay using peripheral blood mononuclear cells (PBMCs).

    Techniques:

    IGRA responses before and after TST administration. (A) IFN-γ responses detected by QFT-GIT in individuals who became positive following TST; (B) SFC numbers in response to ESAT-6 and CFP-10 in individuals who became positive by T-SPOT.TB following TST; (C) SFC numbers in response to ESAT-6 and CFP-10 in individuals who became negative by T-SPOT.TB following TST. Uncertainty zone (grey shaded area); threshold for QFT-GIT positivity (dashed line); upper and lower thresholds for T-SPOT.TB conversion (solid lines). TST = tuberculin skin test; QFT-GIT = QuantiFERON Gold in-Tube; IFN-γ = interferon-gamma; ESAT-6 = early secretory antigenic target-6; CFP-10 = culture filtrate protein-10; IU = international units; SFC = spot forming cells.

    Journal: PLoS ONE

    Article Title: Tuberculin Skin Testing and Treatment Modulates Interferon-Gamma Release Assay Results for Latent Tuberculosis in Migrants

    doi: 10.1371/journal.pone.0097366

    Figure Lengend Snippet: IGRA responses before and after TST administration. (A) IFN-γ responses detected by QFT-GIT in individuals who became positive following TST; (B) SFC numbers in response to ESAT-6 and CFP-10 in individuals who became positive by T-SPOT.TB following TST; (C) SFC numbers in response to ESAT-6 and CFP-10 in individuals who became negative by T-SPOT.TB following TST. Uncertainty zone (grey shaded area); threshold for QFT-GIT positivity (dashed line); upper and lower thresholds for T-SPOT.TB conversion (solid lines). TST = tuberculin skin test; QFT-GIT = QuantiFERON Gold in-Tube; IFN-γ = interferon-gamma; ESAT-6 = early secretory antigenic target-6; CFP-10 = culture filtrate protein-10; IU = international units; SFC = spot forming cells.

    Article Snippet: IGRA testing of recruits in the UK Two commercially available assays were used - The QuantiFERON-TB Gold In-Tube (QFT-GIT; Cellestis, Carnegie, Australia) whole blood enzyme-linked immunosorbent assay (ELISA), and the T-SPOT.TB assay (Oxford Immunotec, Abingdon, UK) enzyme-linked immunospot (ELISPOT) assay using peripheral blood mononuclear cells (PBMCs).

    Techniques:

    The relationship between the magnitude of IFN-γ response among individuals positive by both IGRAs at baseline. Solid line represents linear correlation. QFT-GIT = QuantiFERON Gold in-Tube; IFN-γ = interferon-gamma; ESAT-6 = early secretory antigenic target-6; CFP-10 = culture filtrate protein-10; IU = international units; SFC = spot forming cells.

    Journal: PLoS ONE

    Article Title: Tuberculin Skin Testing and Treatment Modulates Interferon-Gamma Release Assay Results for Latent Tuberculosis in Migrants

    doi: 10.1371/journal.pone.0097366

    Figure Lengend Snippet: The relationship between the magnitude of IFN-γ response among individuals positive by both IGRAs at baseline. Solid line represents linear correlation. QFT-GIT = QuantiFERON Gold in-Tube; IFN-γ = interferon-gamma; ESAT-6 = early secretory antigenic target-6; CFP-10 = culture filtrate protein-10; IU = international units; SFC = spot forming cells.

    Article Snippet: IGRA testing of recruits in the UK Two commercially available assays were used - The QuantiFERON-TB Gold In-Tube (QFT-GIT; Cellestis, Carnegie, Australia) whole blood enzyme-linked immunosorbent assay (ELISA), and the T-SPOT.TB assay (Oxford Immunotec, Abingdon, UK) enzyme-linked immunospot (ELISPOT) assay using peripheral blood mononuclear cells (PBMCs).

    Techniques:

    Study flow diagram of screening and testing for latent tuberculosis infection. At recruitment demographic data, past medical history, previous TB disease, BCG vaccination status and recent TB exposure were all recorded. TST = tuberculin skin test; QFT-GIT = QuantiFERON Gold in-Tube.

    Journal: PLoS ONE

    Article Title: Tuberculin Skin Testing and Treatment Modulates Interferon-Gamma Release Assay Results for Latent Tuberculosis in Migrants

    doi: 10.1371/journal.pone.0097366

    Figure Lengend Snippet: Study flow diagram of screening and testing for latent tuberculosis infection. At recruitment demographic data, past medical history, previous TB disease, BCG vaccination status and recent TB exposure were all recorded. TST = tuberculin skin test; QFT-GIT = QuantiFERON Gold in-Tube.

    Article Snippet: IGRA testing of recruits in the UK Two commercially available assays were used - The QuantiFERON-TB Gold In-Tube (QFT-GIT; Cellestis, Carnegie, Australia) whole blood enzyme-linked immunosorbent assay (ELISA), and the T-SPOT.TB assay (Oxford Immunotec, Abingdon, UK) enzyme-linked immunospot (ELISPOT) assay using peripheral blood mononuclear cells (PBMCs).

    Techniques: Flow Cytometry, Infection