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  • 99
    Millipore tris hcl
    Tris Hcl, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 61632 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore tri reagent
    Tri Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 45305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore tris
    Tris, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 38479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tbst
    Tbst, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 37115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tris hcl
    Tris Hcl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bis tris gels
    Bis Tris Gels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 24196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Molecular Research Center inc tri reagent
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    Tri Reagent, supplied by Molecular Research Center inc, used in various techniques. Bioz Stars score: 92/100, based on 20864 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bis tris gel
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    Bis Tris Gel, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 20760 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore tris buffered saline
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    Tris Buffered Saline, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 18302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tris
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    Tris, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 14380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tri reagent
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    Tri Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam tris buffered saline
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    Tris Buffered Saline, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 10637 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc tris buffered saline
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    Tris Buffered Saline, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 10124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tris buffered saline
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    Tris Buffered Saline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 7131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology tris buffered saline
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    Tris Buffered Saline, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 12205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore tris buffer
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    Tris Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tris glycine gels
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    Tris Glycine Gels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7180 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore tris hcl buffer
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    Tris Hcl Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore m tris hcl
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    M Tris Hcl, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 6423 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore tris buffered saline tbs
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    Tris Buffered Saline Tbs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4911 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher phenol chloroform
    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a <t>Tri-Reagent</t> protocol for the preparation of <t>RNA.</t> miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p
    Phenol Chloroform, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4463 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TBTA  (Cayman)
    N/A
    TBTA is a tertiary amine with three 1 2 3 triazole groups It complexes with and stabilizes copper I to accelerate azide alkyne cycloadditions as used in click chemistry
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    N/A
    50X Tris Acetate EDTA TAE 2 0 M Tris Acetate 0 05 M EDTA pH 8 3 MB 020
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    Image Search Results


    miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a Tri-Reagent protocol for the preparation of RNA. miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p

    Journal: Biochemistry and Biophysics Reports

    Article Title: MicroRNA-424 regulates the expression of CX3CL1 (fractalkine) in human microvascular endothelial cells during Rickettsia rickettsii infection

    doi: 10.1016/j.bbrep.2020.100897

    Figure Lengend Snippet: miR-424-5p (miR-424) expression in R. rickettsii infected endothelial cells. (A) Schematic depicting the binding site of miR-424 at the 3′UTR region of Cx3cl1 mRNA, suggesting Cx3cl1 as a potential target for miR-424. (B) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h and processed using a Tri-Reagent protocol for the preparation of RNA. miR-424 expression was measured by RT-qPCR using a miR-specific Taqman assay. The error bars represent mean ± standard error from three separate experiments. The asterisks indicate significant change (p

    Article Snippet: At different times post-infection, culture medium was removed by gentle aspiration and the cells were lysed directly in TRI reagent® (Molecular Research Center) for RNA isolation [ ].

    Techniques: Expressing, Infection, Binding Assay, Quantitative RT-PCR, TaqMan Assay

    Regulation of Cx3cl1 mRNA expression by miR-424-5p (miR-424). (A) Endothelial cells were transfected with miR-424 mimic (1nM for 24 h) prior to infection with R. rickettsii for 24 h. Cells were then lysed in Tri-reagent for the isolation of RNA and determination of miR-424 expression by a Taqman assay. (B) Cx3cl1 mRNA expression was measured by RT-qPCR using a specific primer pair. (C) CX3CL1 protein expression was measured by western blot using an antibody against CX3CL1. The asterisks indicate statistically significant change (p

    Journal: Biochemistry and Biophysics Reports

    Article Title: MicroRNA-424 regulates the expression of CX3CL1 (fractalkine) in human microvascular endothelial cells during Rickettsia rickettsii infection

    doi: 10.1016/j.bbrep.2020.100897

    Figure Lengend Snippet: Regulation of Cx3cl1 mRNA expression by miR-424-5p (miR-424). (A) Endothelial cells were transfected with miR-424 mimic (1nM for 24 h) prior to infection with R. rickettsii for 24 h. Cells were then lysed in Tri-reagent for the isolation of RNA and determination of miR-424 expression by a Taqman assay. (B) Cx3cl1 mRNA expression was measured by RT-qPCR using a specific primer pair. (C) CX3CL1 protein expression was measured by western blot using an antibody against CX3CL1. The asterisks indicate statistically significant change (p

    Article Snippet: At different times post-infection, culture medium was removed by gentle aspiration and the cells were lysed directly in TRI reagent® (Molecular Research Center) for RNA isolation [ ].

    Techniques: Expressing, Transfection, Infection, Isolation, TaqMan Assay, Quantitative RT-PCR, Western Blot

    CX3CL1 expression in R. rickettsii infected endothelial cells. (A) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h. At each time point post-infection, cells were lysed in Tri-Reagent for the extraction of total RNA, which was subjected to the analysis of Cx3cl1 mRNA expression by RT-qPCR using a gene-specific primer pair. (B) Total protein lysates from R. rickettsii -infected HMECs were prepared in RIPA buffer containing protease inhibitor cocktail and processed for Western blotting using an antibody against CX3CL1 to measure the levels of cellular protein expression. α-tubulin was used as a protein loading control. (C) Quantitation of CX3CL1 protein expression from three independent experiments is presented as the mean ± standard error. The asterisks indicate significant change (p

    Journal: Biochemistry and Biophysics Reports

    Article Title: MicroRNA-424 regulates the expression of CX3CL1 (fractalkine) in human microvascular endothelial cells during Rickettsia rickettsii infection

    doi: 10.1016/j.bbrep.2020.100897

    Figure Lengend Snippet: CX3CL1 expression in R. rickettsii infected endothelial cells. (A) Confluent HMECs were infected with R. rickettsii for 3 h or 24 h. At each time point post-infection, cells were lysed in Tri-Reagent for the extraction of total RNA, which was subjected to the analysis of Cx3cl1 mRNA expression by RT-qPCR using a gene-specific primer pair. (B) Total protein lysates from R. rickettsii -infected HMECs were prepared in RIPA buffer containing protease inhibitor cocktail and processed for Western blotting using an antibody against CX3CL1 to measure the levels of cellular protein expression. α-tubulin was used as a protein loading control. (C) Quantitation of CX3CL1 protein expression from three independent experiments is presented as the mean ± standard error. The asterisks indicate significant change (p

    Article Snippet: At different times post-infection, culture medium was removed by gentle aspiration and the cells were lysed directly in TRI reagent® (Molecular Research Center) for RNA isolation [ ].

    Techniques: Expressing, Infection, Quantitative RT-PCR, Protease Inhibitor, Western Blot, Quantitation Assay