Journal: Journal of Translational Medicine

Article Title: Fn14-Fc suppresses germinal center formation and pathogenic B cells in a lupus mouse model via inhibition of the TWEAK/Fn14 Pathway

doi: 10.1186/s12967-016-0846-4

Figure Lengend Snippet: TWEAK promotes B cell differentiation. a CD19 + B cells isolated from the spleens of sanroque mice were cultured with or without TWEAK (0.1 ng/ml). After 3 days, total RNA was extracted using the TRI reagent. The mRNA levels of various B cell differentiation markers—such as AID , Blimp - 1 , IRF4 —were analyzed by real-time PCR. b Splenocytes, CD4 + T cells, and CD19 + B cells were isolated from the spleens of sanroque mice, total RNA was extracted using TRI reagent, and mRNA levels of Fn14 were analyzed by real-time PCR. c Spleens from the sanroque mice were examined by immunofluorescence staining with monoclonal Abs against B220 ( blue ) and Fn14 ( red ). Data are expressed as means ± SDs. * P

Article Snippet: Total RNA was extracted using the TRI Reagent (Molecular Research Center, Cincinnati, OH, USA).

Techniques: Cell Differentiation, Isolation, Mouse Assay, Cell Culture, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining