Journal: International Journal of Molecular Sciences
Article Title: Deciphering the Functional Composition of Fusogenic Liposomes
Figure Lengend Snippet: Importance of the aromatic component. ( A ) Fluorescence micrographs of CHO cells treated with liposomes containing BODIPY FL-DHPE as aromatic component in fusogenic liposomes (FL) at 0.01 (*) and 0.1 (**) mol/mol concentration as well as in endocytotic liposomes (EL) at the same concentrations (# and ##). Scale bars, 20 µm. ( B ) Dependence of fusion efficiency on dye concentration in FLs (black) and in ELs (red) determined by flow cytometry. The signal intensity median of the whole cell population was plotted vs. BODIPY FL-DHPE molar ratio to the cationic DOTAP amount in the liposomes (n Bodipy FL-DHPE /n DOTAP mol/mol). Measurement points with standard deviations are shown as squares (FL) and circles (EL), respectively. Lines represent linear fits. ( C ) Molecular structures of the chain labelled lipid βBODIPY-C 12 HPC, the head labelled lipid BODIPY FL-DPHE, and the lipophilic membrane dye DiR incorporated in FLs as fluorescent components. The aromatic molecular parts are coloured green and red, representing their spectral emissions. Results for βBODIPY-C 12 .
Article Snippet: The fluorescently labelled lipids N -(4,4-difluoro-5,7-dimethyl-4-bora-3 a ,4 a -diaza- s -indacene-3-propionyl)-1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine (triethylammonium salt) (BODIPY FL-DHPE) and 2-(4,4-difluoro-5-methyl-4-bora-3 a ,4 a -diazas-indacene-3-dodecanoyl)-1-hexadecanoyl- sn -glycero-3-phosphocholine (βBODIPY-C12 HPC), and the lipid analogue DiI(C18)7 (DiR) were ordered from Thermo Scientifics (Eugene, OR, USA).
Techniques: Fluorescence, Concentration Assay, Flow Cytometry, Cytometry