trichostatin a Abcam Search Results


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  • 89
    Abcam trichostatin a
    Chromatin accessibility regulated STAT3 dimer-to-tetramer transition (cpCOMB analysis). ( a ) Chromatin organization in HeLa cells stained with Hoechst 33342 after no treatment, 18 h after addition of 400 nM of <t>trichostatin</t> A (loosened chromatin) and 30 min after addition of 5 μg ml −1 of actinomycin D (compacted chromatin). ( b ) Merged STAT3-GFP and STAT3-mCherry intensity images of control, trichostatin A- and actinomycin D-treated live HeLa cells. Scale bar, 5 μm. ( c ) STAT3-GFP and STAT3-mCherry fluorescence intensity within the plane in which the line scan was acquired. The nuclear boundary is indicated by the Hoechst 33342 stain. ( d ) Pseudocoloured brightness maps for STAT3-GFP in control, trichostatin A- and actinomycin D-treated HeLa cells. Dark green pixels represent monomers, light green pixels dimers and red pixels tetramer. ( e ) cpCOMB carpets in control, trichostatin A- and actinomycin D-treated live HeLa cells. White dashed circles highlight tetramer formation. CTYO, cytoplasm; Max, maximum; Min, minimum; NUC, nucleus.
    Trichostatin A, supplied by Abcam, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trichostatin a/product/Abcam
    Average 89 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    trichostatin a - by Bioz Stars, 2020-01
    89/100 stars
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    sirt1  (Abcam)
    96
    Abcam sirt1
    Treatment of CAECs with sera collected from CR rats up-regulated <t>SIRT1</t> protein expression ( A ; Western blotting) and activity ( B ; data from fluorimetric SIRT1 activity assay). Inset: effect of resveratrol on the activity of recombinant SIRT1 in the presence
    Sirt1, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 2003 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirt1/product/Abcam
    Average 96 stars, based on 2003 article reviews
    Price from $9.99 to $1999.99
    sirt1 - by Bioz Stars, 2020-01
    96/100 stars
      Buy from Supplier

    Image Search Results


    Chromatin accessibility regulated STAT3 dimer-to-tetramer transition (cpCOMB analysis). ( a ) Chromatin organization in HeLa cells stained with Hoechst 33342 after no treatment, 18 h after addition of 400 nM of trichostatin A (loosened chromatin) and 30 min after addition of 5 μg ml −1 of actinomycin D (compacted chromatin). ( b ) Merged STAT3-GFP and STAT3-mCherry intensity images of control, trichostatin A- and actinomycin D-treated live HeLa cells. Scale bar, 5 μm. ( c ) STAT3-GFP and STAT3-mCherry fluorescence intensity within the plane in which the line scan was acquired. The nuclear boundary is indicated by the Hoechst 33342 stain. ( d ) Pseudocoloured brightness maps for STAT3-GFP in control, trichostatin A- and actinomycin D-treated HeLa cells. Dark green pixels represent monomers, light green pixels dimers and red pixels tetramer. ( e ) cpCOMB carpets in control, trichostatin A- and actinomycin D-treated live HeLa cells. White dashed circles highlight tetramer formation. CTYO, cytoplasm; Max, maximum; Min, minimum; NUC, nucleus.

    Journal: Nature Communications

    Article Title: Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness

    doi: 10.1038/ncomms11047

    Figure Lengend Snippet: Chromatin accessibility regulated STAT3 dimer-to-tetramer transition (cpCOMB analysis). ( a ) Chromatin organization in HeLa cells stained with Hoechst 33342 after no treatment, 18 h after addition of 400 nM of trichostatin A (loosened chromatin) and 30 min after addition of 5 μg ml −1 of actinomycin D (compacted chromatin). ( b ) Merged STAT3-GFP and STAT3-mCherry intensity images of control, trichostatin A- and actinomycin D-treated live HeLa cells. Scale bar, 5 μm. ( c ) STAT3-GFP and STAT3-mCherry fluorescence intensity within the plane in which the line scan was acquired. The nuclear boundary is indicated by the Hoechst 33342 stain. ( d ) Pseudocoloured brightness maps for STAT3-GFP in control, trichostatin A- and actinomycin D-treated HeLa cells. Dark green pixels represent monomers, light green pixels dimers and red pixels tetramer. ( e ) cpCOMB carpets in control, trichostatin A- and actinomycin D-treated live HeLa cells. White dashed circles highlight tetramer formation. CTYO, cytoplasm; Max, maximum; Min, minimum; NUC, nucleus.

    Article Snippet: Loosening of chromatin was carried out by treating the HeLa cells transiently transfected with STAT3-GFP and STAT3-mCherry, with trichostatin A (400 nM, Abcam) for 18 h. Compacting of chromatin was carried out by treating the HeLa cells transiently transfected with STAT3-GFP and STAT3-mCherry, with actinomycin D (5 μg ml−1 , a concentration known to stop class III transcription, Sigma Aldrich) for 30 min.

    Techniques: Staining, Fluorescence

    Treatment of CAECs with sera collected from CR rats up-regulated SIRT1 protein expression ( A ; Western blotting) and activity ( B ; data from fluorimetric SIRT1 activity assay). Inset: effect of resveratrol on the activity of recombinant SIRT1 in the presence

    Journal: Mechanisms of ageing and development

    Article Title: Anti-oxidative and anti-inflammatory vasoprotective effects of caloric restriction in aging: role of circulating factors and SIRT1

    doi: 10.1016/j.mad.2009.06.004

    Figure Lengend Snippet: Treatment of CAECs with sera collected from CR rats up-regulated SIRT1 protein expression ( A ; Western blotting) and activity ( B ; data from fluorimetric SIRT1 activity assay). Inset: effect of resveratrol on the activity of recombinant SIRT1 in the presence

    Article Snippet: In brief, this assay is based on the principle that upon NAD-dependent deacetylation of the specific substrate by SIRT1 (in the presence of trichostatin A, a potent inhibitor of SIRT1-independent histone deacetylases), the fluoro-substrate peptide is cleaved by a lysyl endopeptidase, separating the quencher from the fluorophore.

    Techniques: Expressing, Western Blot, Activity Assay, Recombinant