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  • 94
    Millipore transthyretin
    T4 efflux from preloaded 293-BSAT1(orf) cells is monitored with the <t>prealbumin</t> trap technique (Methods). The cells are exposed to fresh medium containing [ 125 I]-T4 for 15 minutes, and either 0 (closed diamonds) or 50 uM E2G (closed squares). The efflux is monitored starting with the addition of prealbumin to the medium at zero time, and the T4 efflux is linear for 10 minutes. The addition of E2G to the medium accelerates the efflux of T4, as demonstrated by the 3.5-fold increase in T4 efflux rate constant.
    Transthyretin, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Agilent technologies transthyretin
    Exogenous <t>transthyretin</t> rescues glomerular integrity and inhibits production of sFlt-1 and sEng in preeclamptic IL-10 −/− mice. A : Histopathological analysis of H E- or periodic acid schiff (PAS)-stained renal tissues from NPS −
    Transthyretin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Abcam transthyretin
    Identification of TTR as a novel interacting protein for the GABA A receptor δ subunit. ( A ) Alignment of the amino-acid sequence of full length mouse <t>transthyretin</t> (NP_038725) with the sequence of the positive clone obtained from our yeast two-hybrid screening. ( B-C ) Co-immunoprecipitation of the HA-tagged TTR and the myc-tagged extracellular domain of the δ-subunit (extra-δ) after co-expression in HEK cells. ( D ) Confocal images showing the co-localization of the α6β3δ-receptors (green) and TTR (red) after co-expressed in HEK cells. The arrows show the coloclization sites. Scale bar, 10 μm. (E) The quantification of colocalization of the transfected TTR and α6β3δ receptors in HEK cell by intensity correlation analysis in Image J. The mean intensity correlation quotient(ICQ) is between 0 and +05, which means they are dependent staining.
    Transthyretin, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Dade Behring prealbumin
    Liver-synthesized proteins and triglycerides. Higher (A) C-reactive protein (CRP) levels after day 17, demonstrating a prolonged inflammatory response in the retinol binding protein (RBP) high group. (B) <t>Prealbumin</t> (PALB) as an important molecule in the carrier system for RBP is correlating with systemic high RBP levels. (C) Significantly higher triglyceride (TG) levels in the high group indicating diminished metabolism and lipolysis. Organ-specific markers for (D, E) renal and (F) liver (F) function were significantly elevated in the RBP high group (data presented as mean ± SEM). BUN, blood urea nitrogen; CRE, creatinine; BIL, bilirubin. Significance: p
    Prealbumin, supplied by Dade Behring, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Agilent technologies rabbit polyclonal anti prealbumin
    T24 antibody specifically reacted with TTR amyloid, but not serum TTR, from FAP patients. A, T24 reactivity was assessed by ELISA. Human serum-derived WT-TTR fibril by acidic treatment ( black circle ), BSA-conjugated TTR peptide ( white circle ), serum from a healthy volunteer ( black square ), and serum from a V30M FAP patient ( white square ) were immobilized on an immunoplate, and the reactivity of T24 against each antigen was analyzed by ELISA. B and C, reactivity of T24 was assessed by Western blotting ( WB ). B, serum from a healthy volunteer or V30M FAP patient was applied to SDS-PAGE under non-reducing conditions and then analyzed by Western blotting with <t>polyclonal</t> <t>anti-prealbumin</t> or T24 antibody. C, serum or extracted amyloids from the heart and kidney from a V30M FAP patient were applied to SDS-PAGE, and then analyzed by Western blotting with T24 antibody.
    Rabbit Polyclonal Anti Prealbumin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Agilent technologies anti human transthyretin ttr
    ( A ) Amyloid plaque in the stomach that stains with Congo red and exhibits apple green birefringence ( A i A ii ). The same plaque stains Thioflavin S positive ( A iii ) and is composed of human <t>transthyretin</t> ( A iv ). The area of co-localization of Thioflavin S and <t>TTR</t> labelling appears yellow ( A v ) and morphometric measurements are carried out with the Image J software ( A vi ), ( B ) Morphometric comparison of amyloid deposition in the two backgrounds. Western blots of human non fibrillar TTR in the transgenic animals of the two backgrounds: in the liver ( C ), the serum ( D ) and stomach tissue ( E ).
    Anti Human Transthyretin Ttr, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies transthyretin ttr
    <t>Transthyretin</t> <t>(TTR)</t> localization in lumbar spinal cord motor neurons. TTR immunoreactivity is reduced in amyotrophic lateral sclerosis (ALS) spinal motor neurons. Human lumbar spinal cord tissues from 16 ALS and eight age-matched non-neurologic disease
    Transthyretin Ttr, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Lee Biosolutions transthyretin ttr
    Asymmetric charge partitioning factor of a) 10+ to 15+ streptavidin (SA), b) 11+ to 17+ <t>transthyretin</t> <t>(TTR),</t> c) 12+ to 17+ hemoglobin (Hb), and d) 17+ to 19+ and 23+ to 27+ C-reactive protein (CRP) upon activation with HCD and low- and high-energy UVPD (1 pulse). The ACPF for Hb is shown as the average for α- and β-subunits. The lab frame collision energy was optimized for each precursor and is equal to 1000 eV for SA (a), 1200 eV for TTR and Hb (b and c), and 2000 eV for CRP (d). The ACPF for purely symmetric charge partitioning (1.0) is shown in each graph. Error bars are equal to standard deviation of replicate data.
    Transthyretin Ttr, supplied by Lee Biosolutions, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    ARVYS transthyretin v30m mutant
    mt NUCB1 has pan amyloid chaperone-like activity. ( A ) hIAPP (30 μM) kinetics of aggregation was tested in absence or in presence of equimolar concentrations of mt NUCB1, or the control protein BSA, and 10 μM Thio-T at 25 °C in quiescent conditions, over 24 h. ( B ) α-synuclein (100 μM) aggregation was measured as end point fluorescence during incubation in absence or in presence of 10 μM mt NUCB1 or BSA, and 10 μM Thio-T, at 37 °C in shaking conditions, for 3 days. ( C ) The <t>transthyretin</t> <t>V30M</t> mutant (10 μM) aggregation was tested in absence or in presence of equimolar concentrations of mt NUCB1 or BSA, and 10 μM Thio-T, at 37 °C in quiescent conditions, over 24 h. ( D ) Composite of representative mt NUCB1- hIAPP, ( E ) mt NUCB1-α-synuclein and ( F ) mt NUCB1-V30M protofibrils purified by SEC and imaged by AFM. For each amyloid, representative protofibrils were selected based on the sample distribution of volumes. Integrated xy scale bar is 40 nm; colorimetric scale bar indicates the height of the particles.
    Transthyretin V30m Mutant, supplied by ARVYS, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Alnylam transthyretin
    mt NUCB1 has pan amyloid chaperone-like activity. ( A ) hIAPP (30 μM) kinetics of aggregation was tested in absence or in presence of equimolar concentrations of mt NUCB1, or the control protein BSA, and 10 μM Thio-T at 25 °C in quiescent conditions, over 24 h. ( B ) α-synuclein (100 μM) aggregation was measured as end point fluorescence during incubation in absence or in presence of 10 μM mt NUCB1 or BSA, and 10 μM Thio-T, at 37 °C in shaking conditions, for 3 days. ( C ) The <t>transthyretin</t> <t>V30M</t> mutant (10 μM) aggregation was tested in absence or in presence of equimolar concentrations of mt NUCB1 or BSA, and 10 μM Thio-T, at 37 °C in quiescent conditions, over 24 h. ( D ) Composite of representative mt NUCB1- hIAPP, ( E ) mt NUCB1-α-synuclein and ( F ) mt NUCB1-V30M protofibrils purified by SEC and imaged by AFM. For each amyloid, representative protofibrils were selected based on the sample distribution of volumes. Integrated xy scale bar is 40 nm; colorimetric scale bar indicates the height of the particles.
    Transthyretin, supplied by Alnylam, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies rabbit anti human transthyretin polyclonal antibody
    Evaluation of TTR deposition in the head of treated flies Representative anti-TTR western-blots of soluble and insoluble material extracted from TTR-A (A) and V30M (B) fly heads. Every sample was extracted from 20 flies and 2.2 μg was loaded per condition. Anti-human <t>transthyretin</t> <t>polyclonal</t> antibody (DAKO) was used to detect transthyretin in the soluble and insoluble fractions. (C) Quantification of four independent western blots. Insoluble and soluble relative amounts were normalized to the total detected signal per condition. These fractions were obtained as described in Materials and Methods.
    Rabbit Anti Human Transthyretin Polyclonal Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Agilent technologies polyclonal rabbit anti human prealbumin ttr
    T24 antibody recognized <t>TTR</t> amyloids in the kidney and heart from FAP patients. T24 reactivity was evaluated by immunohistochemical analysis. Paraffin sections ( A–F and M–R ) or frozen sections ( G–L and S–X ) of the kidney glomerulus tissue ( A–L ) or the heart tissue ( M–X ) from a V30M FAP patient (67-year-old man) were prepared. After Congo red and hematoxylin staining ( C, I, O, and U ), the presence of apple-green birefringence under polarized light confirmed the presence of amyloid deposits ( D, J, P, and V ). For immunohistochemical staining, paraffin-embedded and frozen slides were treated with periodic acid, followed by immunostaining using T24 antibody ( A, G, M, and S ), <t>anti-prealbumin</t> antibody ( B, H, N, and T ), isotype control ( E, K, Q, and W ), or no primary antibody (secondary antibody only) ( F, L, R, and X ). Areas that are stained brown are deposits of TTR amyloids. Bars, 200 μm.
    Polyclonal Rabbit Anti Human Prealbumin Ttr, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Santa Cruz Biotechnology anti transthyretin
    CP explants show typical CP markers. (A–E) Presence of <t>transthyretin</t> (TTR), aquaporin 1 (AQ1), ZO1, Claudin 3 and ICAM-1 with DAPI (blue) staining in CP explants. White arrows indicate the localization of the staining on the apical or basolateral cell membrane (bar 20 μm). Optical thickness of images sections 3 μm. (F) Distribution of F-actin (cyan) detected by phalloidin in ex vivo CP fragment and explants (G) . (H) E-cadherin staining in a CP explant. (I) Expression of the CP epithelial markers, Ttr , lipocalin-type prostaglandin D Synthase ( Ptgds ), transferrin ( Tf ), creatine kinase brain ( Ckb ), occludin ( Ocln ), insulin-like growth factor 2 ( IGF-2 ) and G protein-coupled receptor 125 (GPR125) in native mouse CP (white bars) and CP explant at day 12 (black bars). n = 3 for explant series and n = 4 for CP fragments.
    Anti Transthyretin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Pfizer Inc transthyretin amyloidosis
    Characteristics of the patients included in THAOS as of June 2013. a Five patients had gene polymorphisms and 40 patients had a non-biopsy proven diagnosis of wt-ATTR amyloidosis. b Thirty-two patients had an unknown symptomatic status. ATTR : <t>transthyretin</t> amyloid; n : number of subjects; THAOS : transthyretin amyloidosis outcomes survey; TTR : transthyretin; wt : wild-type.
    Transthyretin Amyloidosis, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GenWay human prealbumin elisa kit
    Characteristics of the patients included in THAOS as of June 2013. a Five patients had gene polymorphisms and 40 patients had a non-biopsy proven diagnosis of wt-ATTR amyloidosis. b Thirty-two patients had an unknown symptomatic status. ATTR : <t>transthyretin</t> amyloid; n : number of subjects; THAOS : transthyretin amyloidosis outcomes survey; TTR : transthyretin; wt : wild-type.
    Human Prealbumin Elisa Kit, supplied by GenWay, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Agilent technologies human transthyretin
    Exogenous <t>transthyretin</t> rescues glomerular integrity and inhibits production of sFlt-1 and sEng in preeclamptic IL-10 −/− mice. A : Histopathological analysis of H E- or periodic acid schiff (PAS)-stained renal tissues from NPS −
    Human Transthyretin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher transthyretin polyclonal antibody
    Exogenous <t>transthyretin</t> rescues glomerular integrity and inhibits production of sFlt-1 and sEng in preeclamptic IL-10 −/− mice. A : Histopathological analysis of H E- or periodic acid schiff (PAS)-stained renal tissues from NPS −
    Transthyretin Polyclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Abcam prealbumin elisa kit
    Exogenous <t>transthyretin</t> rescues glomerular integrity and inhibits production of sFlt-1 and sEng in preeclamptic IL-10 −/− mice. A : Histopathological analysis of H E- or periodic acid schiff (PAS)-stained renal tissues from NPS −
    Prealbumin Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    T4 efflux from preloaded 293-BSAT1(orf) cells is monitored with the prealbumin trap technique (Methods). The cells are exposed to fresh medium containing [ 125 I]-T4 for 15 minutes, and either 0 (closed diamonds) or 50 uM E2G (closed squares). The efflux is monitored starting with the addition of prealbumin to the medium at zero time, and the T4 efflux is linear for 10 minutes. The addition of E2G to the medium accelerates the efflux of T4, as demonstrated by the 3.5-fold increase in T4 efflux rate constant.

    Journal: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

    Article Title: BLOOD-BRAIN BARRIER GENOMICS AND CLONING OF A NOVEL ORGANIC ANION TRANSPORTER

    doi: 10.1038/sj.jcbfm.9600538

    Figure Lengend Snippet: T4 efflux from preloaded 293-BSAT1(orf) cells is monitored with the prealbumin trap technique (Methods). The cells are exposed to fresh medium containing [ 125 I]-T4 for 15 minutes, and either 0 (closed diamonds) or 50 uM E2G (closed squares). The efflux is monitored starting with the addition of prealbumin to the medium at zero time, and the T4 efflux is linear for 10 minutes. The addition of E2G to the medium accelerates the efflux of T4, as demonstrated by the 3.5-fold increase in T4 efflux rate constant.

    Article Snippet: Human plasma prealbumin (Sigma-Aldrich) is a high affinity binder of T4, and this rapid binding prevents any further influx of T4 into the cell.

    Techniques:

    Exogenous transthyretin rescues glomerular integrity and inhibits production of sFlt-1 and sEng in preeclamptic IL-10 −/− mice. A : Histopathological analysis of H E- or periodic acid schiff (PAS)-stained renal tissues from NPS −

    Journal: The American Journal of Pathology

    Article Title: Transthyretin Is Dysregulated in Preeclampsia, and Its Native Form Prevents the Onset of Disease in a Preclinical Mouse Model

    doi: 10.1016/j.ajpath.2013.07.022

    Figure Lengend Snippet: Exogenous transthyretin rescues glomerular integrity and inhibits production of sFlt-1 and sEng in preeclamptic IL-10 −/− mice. A : Histopathological analysis of H E- or periodic acid schiff (PAS)-stained renal tissues from NPS −

    Article Snippet: Similarly, immunoprecipitate or supernatant obtained from serum immunodepleted using transthyretin or isotype antibody (Dako, Glostrup, Denmark), as described later, was injected on gd 10.

    Techniques: Mouse Assay, Staining

    Immunoprecipitated (IP) transthyretin from preeclampsia serum (PES) induces disease features in IL-10 −/− mice. Pregnant mice were injected (i.p.) on gd 10 with transthyretin immunoprecipitate obtained from 100 μL normal pregnancy

    Journal: The American Journal of Pathology

    Article Title: Transthyretin Is Dysregulated in Preeclampsia, and Its Native Form Prevents the Onset of Disease in a Preclinical Mouse Model

    doi: 10.1016/j.ajpath.2013.07.022

    Figure Lengend Snippet: Immunoprecipitated (IP) transthyretin from preeclampsia serum (PES) induces disease features in IL-10 −/− mice. Pregnant mice were injected (i.p.) on gd 10 with transthyretin immunoprecipitate obtained from 100 μL normal pregnancy

    Article Snippet: Similarly, immunoprecipitate or supernatant obtained from serum immunodepleted using transthyretin or isotype antibody (Dako, Glostrup, Denmark), as described later, was injected on gd 10.

    Techniques: Immunoprecipitation, Mouse Assay, Injection

    Transthyretin in preeclampsia is dysregulated and forms aggregates. A : Analysis of serum transthyretin in normal pregnancy serum (NPS) and preeclampsia serum (PES) by ELISA. Transthyretin is present at significantly reduced levels in PES ( P

    Journal: The American Journal of Pathology

    Article Title: Transthyretin Is Dysregulated in Preeclampsia, and Its Native Form Prevents the Onset of Disease in a Preclinical Mouse Model

    doi: 10.1016/j.ajpath.2013.07.022

    Figure Lengend Snippet: Transthyretin in preeclampsia is dysregulated and forms aggregates. A : Analysis of serum transthyretin in normal pregnancy serum (NPS) and preeclampsia serum (PES) by ELISA. Transthyretin is present at significantly reduced levels in PES ( P

    Article Snippet: Similarly, immunoprecipitate or supernatant obtained from serum immunodepleted using transthyretin or isotype antibody (Dako, Glostrup, Denmark), as described later, was injected on gd 10.

    Techniques: Enzyme-linked Immunosorbent Assay

    Transthyretin binds to soluble endoglin and its possible mode of action. A : Far Western blot showing the binding of transthyretin with sEng and RBP-4 (red circle) and lack of binding to sFlt-1. Purified sFlt-1, sEng, and RBP-4 were used as prey proteins,

    Journal: The American Journal of Pathology

    Article Title: Transthyretin Is Dysregulated in Preeclampsia, and Its Native Form Prevents the Onset of Disease in a Preclinical Mouse Model

    doi: 10.1016/j.ajpath.2013.07.022

    Figure Lengend Snippet: Transthyretin binds to soluble endoglin and its possible mode of action. A : Far Western blot showing the binding of transthyretin with sEng and RBP-4 (red circle) and lack of binding to sFlt-1. Purified sFlt-1, sEng, and RBP-4 were used as prey proteins,

    Article Snippet: Similarly, immunoprecipitate or supernatant obtained from serum immunodepleted using transthyretin or isotype antibody (Dako, Glostrup, Denmark), as described later, was injected on gd 10.

    Techniques: Far Western Blot, Binding Assay, Purification

    Exogenous transthyretin inhibits preeclampsia-associated features in vitro and in vivo . A : Serum-induced and transthyretin-modified endovascular interaction between endothelial cells (human umbilical cord endothelial cells, cell tracker

    Journal: The American Journal of Pathology

    Article Title: Transthyretin Is Dysregulated in Preeclampsia, and Its Native Form Prevents the Onset of Disease in a Preclinical Mouse Model

    doi: 10.1016/j.ajpath.2013.07.022

    Figure Lengend Snippet: Exogenous transthyretin inhibits preeclampsia-associated features in vitro and in vivo . A : Serum-induced and transthyretin-modified endovascular interaction between endothelial cells (human umbilical cord endothelial cells, cell tracker

    Article Snippet: Similarly, immunoprecipitate or supernatant obtained from serum immunodepleted using transthyretin or isotype antibody (Dako, Glostrup, Denmark), as described later, was injected on gd 10.

    Techniques: In Vitro, In Vivo, Modification

    Surface enhanced laser desorption ionization-time-of-flight (SELDI-TOF) and biochemical analyses of transthyretin in preeclampsia serum. A : Preeclampsia serum (PES) ( n = 53) and normal pregnancy serum (NPS) ( n = 16) were analyzed by SELDI-TOF in the molecular

    Journal: The American Journal of Pathology

    Article Title: Transthyretin Is Dysregulated in Preeclampsia, and Its Native Form Prevents the Onset of Disease in a Preclinical Mouse Model

    doi: 10.1016/j.ajpath.2013.07.022

    Figure Lengend Snippet: Surface enhanced laser desorption ionization-time-of-flight (SELDI-TOF) and biochemical analyses of transthyretin in preeclampsia serum. A : Preeclampsia serum (PES) ( n = 53) and normal pregnancy serum (NPS) ( n = 16) were analyzed by SELDI-TOF in the molecular

    Article Snippet: Similarly, immunoprecipitate or supernatant obtained from serum immunodepleted using transthyretin or isotype antibody (Dako, Glostrup, Denmark), as described later, was injected on gd 10.

    Techniques:

    Transthyretin-immunodepleted preeclampsia serum (PES) fails to induce preeclampsia-like features. Transthyretin-depleted PES (100 μL) was injected on gd10 in IL-10 −/− mice, and its effects on fetal size, blood pressure, proteinuria,

    Journal: The American Journal of Pathology

    Article Title: Transthyretin Is Dysregulated in Preeclampsia, and Its Native Form Prevents the Onset of Disease in a Preclinical Mouse Model

    doi: 10.1016/j.ajpath.2013.07.022

    Figure Lengend Snippet: Transthyretin-immunodepleted preeclampsia serum (PES) fails to induce preeclampsia-like features. Transthyretin-depleted PES (100 μL) was injected on gd10 in IL-10 −/− mice, and its effects on fetal size, blood pressure, proteinuria,

    Article Snippet: Similarly, immunoprecipitate or supernatant obtained from serum immunodepleted using transthyretin or isotype antibody (Dako, Glostrup, Denmark), as described later, was injected on gd 10.

    Techniques: Injection, Mouse Assay

    PLS-DA Analysis of CSF Protein/Peptide Profiles from an Independent Validation Sample Set Containing 17 First-Onset, Drug-Naïve Schizophrenia Patients and 40 Healthy Volunteers The demographic details of this sample set are listed in Table 2 . The PLS-DA scores plot shows a separation between patients (in red) and healthy volunteers (in blue). The loadings plot indicates similar key variables (e.g., the 3,959-Da VGF23–62 peptide [in red] and transthyretin protein signals between 13,600 and 14,000 [in blue]) identified in the first experiment (see Figure 1 ).

    Journal: PLoS Medicine

    Article Title: Disease Biomarkers in Cerebrospinal Fluid of Patients with First-Onset Psychosis

    doi: 10.1371/journal.pmed.0030428

    Figure Lengend Snippet: PLS-DA Analysis of CSF Protein/Peptide Profiles from an Independent Validation Sample Set Containing 17 First-Onset, Drug-Naïve Schizophrenia Patients and 40 Healthy Volunteers The demographic details of this sample set are listed in Table 2 . The PLS-DA scores plot shows a separation between patients (in red) and healthy volunteers (in blue). The loadings plot indicates similar key variables (e.g., the 3,959-Da VGF23–62 peptide [in red] and transthyretin protein signals between 13,600 and 14,000 [in blue]) identified in the first experiment (see Figure 1 ).

    Article Snippet: After washing with washing buffer (0.03% Tween 20 in PBS), the plates were blocked with 100 μl of 5% skimmed-milk powder for 60 min. Transthyretin antibody (100 μl) (DakoCytomation, Glostrup, Denmark, 1:500 diluted in 2.5% skimmed-milk powder) was incubated in 96-well plates for 60 min.

    Techniques:

    Down-Regulation of Three Different Forms of Transthyretin in CSF from First-Onset, Drug-Naïve Schizophrenia Patients Examples of CSF spectra from healthy volunteers and patients with schizophrenia within 6–17 kDa are shown in (A). The peak cluster indicated (red cursor) is enlarged in (B). Statistical details of each sub-peak are listed in the table presented just below (B). On-chip reduction of CSF peptide/protein with β-mecaptoethanol at room temperature showed that the three peaks 13,741, 13,875, and 13,923 Da were reduced into a single peak (C), suggesting that they are different S -cysteinylated derivatives of the same protein. To identify the protein, CSF samples from a healthy volunteer and a patient with schizophrenia were applied to an anion exchanger column (HyperD, Ciphergen Biosystems) and eluted with pH 9–pH 3 buffers. A major ~14–15-kDa band was eluted in the pH 3 fraction (D, left panel). The protein was identified as transthyretin using LC-MS/MS (D, right panel) and the sequence coverage is shown in red. Note, that all the peptides shown have an E- score of

    Journal: PLoS Medicine

    Article Title: Disease Biomarkers in Cerebrospinal Fluid of Patients with First-Onset Psychosis

    doi: 10.1371/journal.pmed.0030428

    Figure Lengend Snippet: Down-Regulation of Three Different Forms of Transthyretin in CSF from First-Onset, Drug-Naïve Schizophrenia Patients Examples of CSF spectra from healthy volunteers and patients with schizophrenia within 6–17 kDa are shown in (A). The peak cluster indicated (red cursor) is enlarged in (B). Statistical details of each sub-peak are listed in the table presented just below (B). On-chip reduction of CSF peptide/protein with β-mecaptoethanol at room temperature showed that the three peaks 13,741, 13,875, and 13,923 Da were reduced into a single peak (C), suggesting that they are different S -cysteinylated derivatives of the same protein. To identify the protein, CSF samples from a healthy volunteer and a patient with schizophrenia were applied to an anion exchanger column (HyperD, Ciphergen Biosystems) and eluted with pH 9–pH 3 buffers. A major ~14–15-kDa band was eluted in the pH 3 fraction (D, left panel). The protein was identified as transthyretin using LC-MS/MS (D, right panel) and the sequence coverage is shown in red. Note, that all the peptides shown have an E- score of

    Article Snippet: After washing with washing buffer (0.03% Tween 20 in PBS), the plates were blocked with 100 μl of 5% skimmed-milk powder for 60 min. Transthyretin antibody (100 μl) (DakoCytomation, Glostrup, Denmark, 1:500 diluted in 2.5% skimmed-milk powder) was incubated in 96-well plates for 60 min.

    Techniques: Chromatin Immunoprecipitation, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Sequencing

    Transthyretin Levels in Sera of First-Onset, Drug-Naïve Schizophrenia Patients and Prefrontal Cortex Post-Mortem Tissue from Schizophrenia Patients The serum samples from the same patients whose CSF protein profiles were measured in Figures 1 and 6 were included in this study. Figure 5 A shows serum tranythyretin levels in patients with schizophrenia significantly decreased by ~15% compared to control subjects. Data are shown in mean ± standard deviation, and the asterisk denotes p = 0.0007 ( t -test). Figure 5 B indicates no correlation between serum transthyretin and CSF SELDI signals from one of the transthyretin isoforms ( m/z = 13,741) in the second sample set (for demographics, see Table 2 ). Similar results were found when comparing with signals from other isoforms in CSF (unpublished data). Figure 5 C shows an ~40% decrease of transthyretin expression in prefrontal cortex of five patients with schizophrenia and in five control subjects. For demographic details, see Table 5 .

    Journal: PLoS Medicine

    Article Title: Disease Biomarkers in Cerebrospinal Fluid of Patients with First-Onset Psychosis

    doi: 10.1371/journal.pmed.0030428

    Figure Lengend Snippet: Transthyretin Levels in Sera of First-Onset, Drug-Naïve Schizophrenia Patients and Prefrontal Cortex Post-Mortem Tissue from Schizophrenia Patients The serum samples from the same patients whose CSF protein profiles were measured in Figures 1 and 6 were included in this study. Figure 5 A shows serum tranythyretin levels in patients with schizophrenia significantly decreased by ~15% compared to control subjects. Data are shown in mean ± standard deviation, and the asterisk denotes p = 0.0007 ( t -test). Figure 5 B indicates no correlation between serum transthyretin and CSF SELDI signals from one of the transthyretin isoforms ( m/z = 13,741) in the second sample set (for demographics, see Table 2 ). Similar results were found when comparing with signals from other isoforms in CSF (unpublished data). Figure 5 C shows an ~40% decrease of transthyretin expression in prefrontal cortex of five patients with schizophrenia and in five control subjects. For demographic details, see Table 5 .

    Article Snippet: After washing with washing buffer (0.03% Tween 20 in PBS), the plates were blocked with 100 μl of 5% skimmed-milk powder for 60 min. Transthyretin antibody (100 μl) (DakoCytomation, Glostrup, Denmark, 1:500 diluted in 2.5% skimmed-milk powder) was incubated in 96-well plates for 60 min.

    Techniques: Standard Deviation, Expressing

    Identification of TTR as a novel interacting protein for the GABA A receptor δ subunit. ( A ) Alignment of the amino-acid sequence of full length mouse transthyretin (NP_038725) with the sequence of the positive clone obtained from our yeast two-hybrid screening. ( B-C ) Co-immunoprecipitation of the HA-tagged TTR and the myc-tagged extracellular domain of the δ-subunit (extra-δ) after co-expression in HEK cells. ( D ) Confocal images showing the co-localization of the α6β3δ-receptors (green) and TTR (red) after co-expressed in HEK cells. The arrows show the coloclization sites. Scale bar, 10 μm. (E) The quantification of colocalization of the transfected TTR and α6β3δ receptors in HEK cell by intensity correlation analysis in Image J. The mean intensity correlation quotient(ICQ) is between 0 and +05, which means they are dependent staining.

    Journal: PLoS ONE

    Article Title: Identification of transthyretin as a novel interacting partner for the δ subunit of GABAA receptors

    doi: 10.1371/journal.pone.0210094

    Figure Lengend Snippet: Identification of TTR as a novel interacting protein for the GABA A receptor δ subunit. ( A ) Alignment of the amino-acid sequence of full length mouse transthyretin (NP_038725) with the sequence of the positive clone obtained from our yeast two-hybrid screening. ( B-C ) Co-immunoprecipitation of the HA-tagged TTR and the myc-tagged extracellular domain of the δ-subunit (extra-δ) after co-expression in HEK cells. ( D ) Confocal images showing the co-localization of the α6β3δ-receptors (green) and TTR (red) after co-expressed in HEK cells. The arrows show the coloclization sites. Scale bar, 10 μm. (E) The quantification of colocalization of the transfected TTR and α6β3δ receptors in HEK cell by intensity correlation analysis in Image J. The mean intensity correlation quotient(ICQ) is between 0 and +05, which means they are dependent staining.

    Article Snippet: For live cell staining, cultured cerebellar or cotical neurons (9–12 days in vitro) were incubated in bath solution (128 mM NaCl, 30 mM glucose, 25 mM HEPES, 2 mM KCl, 2 mM CaCl2 and 1 mM MgCl2 (320 mOsm, pH 7.4) with primary anti-GABAA -R δ subunit or anti-transthyretin antibodies (Abcam) at 4°C for 1 hr, followed by washing with phosphate-buffered saline (PBS) and fixed for 15 min in 4% paraformaldehyde (PFA).

    Techniques: Sequencing, Two Hybrid Screening, Immunoprecipitation, Expressing, Transfection, Staining

    Immunohistochemical staining for TTR. Mass spectrometry revealed upregulation of transthyretin (TTR) which was further characterized with immunohistochemistry. Staining for TTR revealed that TTR was present in all retinal layers and in the choroid. A strong reaction for TTR was seen in the retinal vessels regardless of bevacizumab intervention. A : In branch retinal vein occlusion (BRVO) with bevacizumab intervention, a strong reaction for TTR was observed in the choroid. In the choroid, the reaction for TTR was stronger in BRVO with bevacizumab intervention ( A ) compared to BRVO without bevacizumab intervention ( B ). Therefore, the choroid may be the source of increased TTR in BRVO with bevacizumab intervention. Scale bar = 50 µm. Reaction color: brown. Abbreviations: ILM: inner limiting membrane; NFL: nerve fiber layer; GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer; PRL: photoreceptor layer. RPE: retinal pigment epithelium. CHO: choroid.

    Journal: Molecular Vision

    Article Title: Intravitreal bevacizumab upregulates transthyretin in experimental branch retinal vein occlusion

    doi:

    Figure Lengend Snippet: Immunohistochemical staining for TTR. Mass spectrometry revealed upregulation of transthyretin (TTR) which was further characterized with immunohistochemistry. Staining for TTR revealed that TTR was present in all retinal layers and in the choroid. A strong reaction for TTR was seen in the retinal vessels regardless of bevacizumab intervention. A : In branch retinal vein occlusion (BRVO) with bevacizumab intervention, a strong reaction for TTR was observed in the choroid. In the choroid, the reaction for TTR was stronger in BRVO with bevacizumab intervention ( A ) compared to BRVO without bevacizumab intervention ( B ). Therefore, the choroid may be the source of increased TTR in BRVO with bevacizumab intervention. Scale bar = 50 µm. Reaction color: brown. Abbreviations: ILM: inner limiting membrane; NFL: nerve fiber layer; GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer; PRL: photoreceptor layer. RPE: retinal pigment epithelium. CHO: choroid.

    Article Snippet: A polyclonal IgG antibody directed at TTR (ab9015, Abcam) was used for immunohistochemistry.

    Techniques: Immunohistochemistry, Staining, Mass Spectrometry

    Scatter plots of concentrations of CA125, transthyretin, and apolipoprotein A1. (A) healthy controls, benign ovarian disease and ovarian cancer patients (B) tumor stages in ovarian cancer patients (C) different histological subtypes in ovarian cancer patients. P value over a group denotes statistical significance of differences between each group and healthy controls. Each ovarian cancer subjects was compared with healthy controls. N, healthy controls; BE, benign ovarian disease; C, clear cell; E, endometrioid; G, granulosa cell; M, mucinous; S, serous; O, other.

    Journal: PLoS ONE

    Article Title: Development of Multiplexed Bead-Based Immunoassays for the Detection of Early Stage Ovarian Cancer Using a Combination of Serum Biomarkers

    doi: 10.1371/journal.pone.0044960

    Figure Lengend Snippet: Scatter plots of concentrations of CA125, transthyretin, and apolipoprotein A1. (A) healthy controls, benign ovarian disease and ovarian cancer patients (B) tumor stages in ovarian cancer patients (C) different histological subtypes in ovarian cancer patients. P value over a group denotes statistical significance of differences between each group and healthy controls. Each ovarian cancer subjects was compared with healthy controls. N, healthy controls; BE, benign ovarian disease; C, clear cell; E, endometrioid; G, granulosa cell; M, mucinous; S, serous; O, other.

    Article Snippet: Following the addition of 0.5 μg of each antibody [anti-CA125 (Fitzgerald Industries International, Inc., Concord, MA), anti-transthyretin (Abcam), and anti-apolipoprotein A1 (Fizgerald Industries International, Inc.)] in each tube, the tubes were incubated for 2 h on a shaker, which was protected from the light.

    Techniques:

    ROC (receiver operator characteristic) discriminating ovarian cancer patients from benign ovarian disease patients. The curves shown were obtained by processing quantified raw data by SigmaPlot 12.0 version software and the sensitivity/specificity values were predicted from the area under the curves and the calculated data. ROC curves for CA125, transthyretin, and apolipoprotein A1 alone: (A) benign patients versus patients with ovarian cancer; (B) benign patients versus patients with stages I to II ovarian cancer; (C) benign patients versus patients with stages III to IV ovarian cancer.

    Journal: PLoS ONE

    Article Title: Development of Multiplexed Bead-Based Immunoassays for the Detection of Early Stage Ovarian Cancer Using a Combination of Serum Biomarkers

    doi: 10.1371/journal.pone.0044960

    Figure Lengend Snippet: ROC (receiver operator characteristic) discriminating ovarian cancer patients from benign ovarian disease patients. The curves shown were obtained by processing quantified raw data by SigmaPlot 12.0 version software and the sensitivity/specificity values were predicted from the area under the curves and the calculated data. ROC curves for CA125, transthyretin, and apolipoprotein A1 alone: (A) benign patients versus patients with ovarian cancer; (B) benign patients versus patients with stages I to II ovarian cancer; (C) benign patients versus patients with stages III to IV ovarian cancer.

    Article Snippet: Following the addition of 0.5 μg of each antibody [anti-CA125 (Fitzgerald Industries International, Inc., Concord, MA), anti-transthyretin (Abcam), and anti-apolipoprotein A1 (Fizgerald Industries International, Inc.)] in each tube, the tubes were incubated for 2 h on a shaker, which was protected from the light.

    Techniques: Software

    Liver-synthesized proteins and triglycerides. Higher (A) C-reactive protein (CRP) levels after day 17, demonstrating a prolonged inflammatory response in the retinol binding protein (RBP) high group. (B) Prealbumin (PALB) as an important molecule in the carrier system for RBP is correlating with systemic high RBP levels. (C) Significantly higher triglyceride (TG) levels in the high group indicating diminished metabolism and lipolysis. Organ-specific markers for (D, E) renal and (F) liver (F) function were significantly elevated in the RBP high group (data presented as mean ± SEM). BUN, blood urea nitrogen; CRE, creatinine; BIL, bilirubin. Significance: p

    Journal: JPEN. Journal of parenteral and enteral nutrition

    Article Title: Retinol Binding Protein: Marker for Insulin Resistance and Inflammation Postburn?

    doi: 10.1177/0148607111413901

    Figure Lengend Snippet: Liver-synthesized proteins and triglycerides. Higher (A) C-reactive protein (CRP) levels after day 17, demonstrating a prolonged inflammatory response in the retinol binding protein (RBP) high group. (B) Prealbumin (PALB) as an important molecule in the carrier system for RBP is correlating with systemic high RBP levels. (C) Significantly higher triglyceride (TG) levels in the high group indicating diminished metabolism and lipolysis. Organ-specific markers for (D, E) renal and (F) liver (F) function were significantly elevated in the RBP high group (data presented as mean ± SEM). BUN, blood urea nitrogen; CRE, creatinine; BIL, bilirubin. Significance: p

    Article Snippet: Serum proteins C-reactive protein (CRP), osteocalcin, RBP, and prealbumin were determined using high-performance liquid chromatography, nephelometry (BNII; Plasma Protein Analyzer, Dade Behring, Bowie, MD), and enzyme-linked immunosorbent assay techniques.

    Techniques: Synthesized, Binding Assay

    T24 antibody specifically reacted with TTR amyloid, but not serum TTR, from FAP patients. A, T24 reactivity was assessed by ELISA. Human serum-derived WT-TTR fibril by acidic treatment ( black circle ), BSA-conjugated TTR peptide ( white circle ), serum from a healthy volunteer ( black square ), and serum from a V30M FAP patient ( white square ) were immobilized on an immunoplate, and the reactivity of T24 against each antigen was analyzed by ELISA. B and C, reactivity of T24 was assessed by Western blotting ( WB ). B, serum from a healthy volunteer or V30M FAP patient was applied to SDS-PAGE under non-reducing conditions and then analyzed by Western blotting with polyclonal anti-prealbumin or T24 antibody. C, serum or extracted amyloids from the heart and kidney from a V30M FAP patient were applied to SDS-PAGE, and then analyzed by Western blotting with T24 antibody.

    Journal: The Journal of Biological Chemistry

    Article Title: Novel Antibody for the Treatment of Transthyretin Amyloidosis *

    doi: 10.1074/jbc.M116.738138

    Figure Lengend Snippet: T24 antibody specifically reacted with TTR amyloid, but not serum TTR, from FAP patients. A, T24 reactivity was assessed by ELISA. Human serum-derived WT-TTR fibril by acidic treatment ( black circle ), BSA-conjugated TTR peptide ( white circle ), serum from a healthy volunteer ( black square ), and serum from a V30M FAP patient ( white square ) were immobilized on an immunoplate, and the reactivity of T24 against each antigen was analyzed by ELISA. B and C, reactivity of T24 was assessed by Western blotting ( WB ). B, serum from a healthy volunteer or V30M FAP patient was applied to SDS-PAGE under non-reducing conditions and then analyzed by Western blotting with polyclonal anti-prealbumin or T24 antibody. C, serum or extracted amyloids from the heart and kidney from a V30M FAP patient were applied to SDS-PAGE, and then analyzed by Western blotting with T24 antibody.

    Article Snippet: To detect TTRs, rabbit polyclonal anti-prealbumin and goat anti-rabbit IgG antibodies conjugated with HRP (Dako) were used for 1 h at room temperature as the primary and secondary antibodies, respectively.

    Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Western Blot, SDS Page

    ( A ) Amyloid plaque in the stomach that stains with Congo red and exhibits apple green birefringence ( A i A ii ). The same plaque stains Thioflavin S positive ( A iii ) and is composed of human transthyretin ( A iv ). The area of co-localization of Thioflavin S and TTR labelling appears yellow ( A v ) and morphometric measurements are carried out with the Image J software ( A vi ), ( B ) Morphometric comparison of amyloid deposition in the two backgrounds. Western blots of human non fibrillar TTR in the transgenic animals of the two backgrounds: in the liver ( C ), the serum ( D ) and stomach tissue ( E ).

    Journal: Biochemistry and Biophysics Reports

    Article Title: Genetic background modifies amyloidosis in a mouse model of ATTR neuropathy

    doi: 10.1016/j.bbrep.2016.08.005

    Figure Lengend Snippet: ( A ) Amyloid plaque in the stomach that stains with Congo red and exhibits apple green birefringence ( A i A ii ). The same plaque stains Thioflavin S positive ( A iii ) and is composed of human transthyretin ( A iv ). The area of co-localization of Thioflavin S and TTR labelling appears yellow ( A v ) and morphometric measurements are carried out with the Image J software ( A vi ), ( B ) Morphometric comparison of amyloid deposition in the two backgrounds. Western blots of human non fibrillar TTR in the transgenic animals of the two backgrounds: in the liver ( C ), the serum ( D ) and stomach tissue ( E ).

    Article Snippet: The membranes were then incubated overnight at 4 °C with anti-human Transthyretin (TTR) (DAKO A000202) at a dilution of 1/2,000.

    Techniques: Software, Western Blot, Transgenic Assay

    Transthyretin (TTR) localization in lumbar spinal cord motor neurons. TTR immunoreactivity is reduced in amyotrophic lateral sclerosis (ALS) spinal motor neurons. Human lumbar spinal cord tissues from 16 ALS and eight age-matched non-neurologic disease

    Journal:

    Article Title: Proteomic profiling of cerebrospinal fluid identifies biomarkers for amyotrophic lateral sclerosis

    doi: 10.1111/j.1471-4159.2005.03478.x

    Figure Lengend Snippet: Transthyretin (TTR) localization in lumbar spinal cord motor neurons. TTR immunoreactivity is reduced in amyotrophic lateral sclerosis (ALS) spinal motor neurons. Human lumbar spinal cord tissues from 16 ALS and eight age-matched non-neurologic disease

    Article Snippet: Anti-rabbit polyclonal antibodies against cystatin C (DAKO, Carpinteria, CA, USA) and transthyretin (TTR) (DAKO) were used for these studies.

    Techniques:

    Asymmetric charge partitioning factor of a) 10+ to 15+ streptavidin (SA), b) 11+ to 17+ transthyretin (TTR), c) 12+ to 17+ hemoglobin (Hb), and d) 17+ to 19+ and 23+ to 27+ C-reactive protein (CRP) upon activation with HCD and low- and high-energy UVPD (1 pulse). The ACPF for Hb is shown as the average for α- and β-subunits. The lab frame collision energy was optimized for each precursor and is equal to 1000 eV for SA (a), 1200 eV for TTR and Hb (b and c), and 2000 eV for CRP (d). The ACPF for purely symmetric charge partitioning (1.0) is shown in each graph. Error bars are equal to standard deviation of replicate data.

    Journal: Physical chemistry chemical physics : PCCP

    Article Title: Impact of charge state on 193 nm ultraviolet photodissociation of protein complexes

    doi: 10.1039/c9cp01144g

    Figure Lengend Snippet: Asymmetric charge partitioning factor of a) 10+ to 15+ streptavidin (SA), b) 11+ to 17+ transthyretin (TTR), c) 12+ to 17+ hemoglobin (Hb), and d) 17+ to 19+ and 23+ to 27+ C-reactive protein (CRP) upon activation with HCD and low- and high-energy UVPD (1 pulse). The ACPF for Hb is shown as the average for α- and β-subunits. The lab frame collision energy was optimized for each precursor and is equal to 1000 eV for SA (a), 1200 eV for TTR and Hb (b and c), and 2000 eV for CRP (d). The ACPF for purely symmetric charge partitioning (1.0) is shown in each graph. Error bars are equal to standard deviation of replicate data.

    Article Snippet: Bovine Cu/Zn superoxide dismutase (SOD) was obtained from MP Biomedicals (Santa Ana, CA), streptavidin (SA) from ProteoChem (Hurricane, UT), human hemoglobin (Hb) from Sigma Aldrich (St. Louis, MO), transthyretin (TTR) from Lee BioSolutions (Maryland Heights, MO), and C-reactive protein (CRP) from EMD Millipore (Burlington, MA).

    Techniques: Activation Assay, Standard Deviation

    mt NUCB1 has pan amyloid chaperone-like activity. ( A ) hIAPP (30 μM) kinetics of aggregation was tested in absence or in presence of equimolar concentrations of mt NUCB1, or the control protein BSA, and 10 μM Thio-T at 25 °C in quiescent conditions, over 24 h. ( B ) α-synuclein (100 μM) aggregation was measured as end point fluorescence during incubation in absence or in presence of 10 μM mt NUCB1 or BSA, and 10 μM Thio-T, at 37 °C in shaking conditions, for 3 days. ( C ) The transthyretin V30M mutant (10 μM) aggregation was tested in absence or in presence of equimolar concentrations of mt NUCB1 or BSA, and 10 μM Thio-T, at 37 °C in quiescent conditions, over 24 h. ( D ) Composite of representative mt NUCB1- hIAPP, ( E ) mt NUCB1-α-synuclein and ( F ) mt NUCB1-V30M protofibrils purified by SEC and imaged by AFM. For each amyloid, representative protofibrils were selected based on the sample distribution of volumes. Integrated xy scale bar is 40 nm; colorimetric scale bar indicates the height of the particles.

    Journal: Scientific Reports

    Article Title: Nucleobindin 1 binds to multiple types of pre-fibrillar amyloid and inhibits fibrillization

    doi: 10.1038/srep42880

    Figure Lengend Snippet: mt NUCB1 has pan amyloid chaperone-like activity. ( A ) hIAPP (30 μM) kinetics of aggregation was tested in absence or in presence of equimolar concentrations of mt NUCB1, or the control protein BSA, and 10 μM Thio-T at 25 °C in quiescent conditions, over 24 h. ( B ) α-synuclein (100 μM) aggregation was measured as end point fluorescence during incubation in absence or in presence of 10 μM mt NUCB1 or BSA, and 10 μM Thio-T, at 37 °C in shaking conditions, for 3 days. ( C ) The transthyretin V30M mutant (10 μM) aggregation was tested in absence or in presence of equimolar concentrations of mt NUCB1 or BSA, and 10 μM Thio-T, at 37 °C in quiescent conditions, over 24 h. ( D ) Composite of representative mt NUCB1- hIAPP, ( E ) mt NUCB1-α-synuclein and ( F ) mt NUCB1-V30M protofibrils purified by SEC and imaged by AFM. For each amyloid, representative protofibrils were selected based on the sample distribution of volumes. Integrated xy scale bar is 40 nm; colorimetric scale bar indicates the height of the particles.

    Article Snippet: On the day of the experiment, the peptide was solubilized in 20 mM sodium phosphate buffer, pH 7.6. α-Synuclein (Bioneer) was solubilized in PBS; the transthyretin V30M mutant (Arvys Proteins) was diluted in 10 mM sodium phosphate, pH 7.6, 100 mM KCl, 1 mM EDTA.

    Techniques: Activity Assay, Fluorescence, Incubation, Mutagenesis, Purification, Size-exclusion Chromatography

    Evaluation of TTR deposition in the head of treated flies Representative anti-TTR western-blots of soluble and insoluble material extracted from TTR-A (A) and V30M (B) fly heads. Every sample was extracted from 20 flies and 2.2 μg was loaded per condition. Anti-human transthyretin polyclonal antibody (DAKO) was used to detect transthyretin in the soluble and insoluble fractions. (C) Quantification of four independent western blots. Insoluble and soluble relative amounts were normalized to the total detected signal per condition. These fractions were obtained as described in Materials and Methods.

    Journal: bioRxiv

    Article Title: Assessment of the effects of transthyretin peptide inhibitors in Drosophila models of neuropathic ATTR

    doi: 10.1101/354555

    Figure Lengend Snippet: Evaluation of TTR deposition in the head of treated flies Representative anti-TTR western-blots of soluble and insoluble material extracted from TTR-A (A) and V30M (B) fly heads. Every sample was extracted from 20 flies and 2.2 μg was loaded per condition. Anti-human transthyretin polyclonal antibody (DAKO) was used to detect transthyretin in the soluble and insoluble fractions. (C) Quantification of four independent western blots. Insoluble and soluble relative amounts were normalized to the total detected signal per condition. These fractions were obtained as described in Materials and Methods.

    Article Snippet: ANTIBODIESAntibodies used were rabbit anti-human transthyretin polyclonal antibody (DAKO, Agilent Technologies; 1:2,000) and horseradish peroxidase-conjugated goat anti-rabbit IgG antibody (DAKO, Agilent Technologies; 1:5,000).

    Techniques: Western Blot

    T24 antibody recognized TTR amyloids in the kidney and heart from FAP patients. T24 reactivity was evaluated by immunohistochemical analysis. Paraffin sections ( A–F and M–R ) or frozen sections ( G–L and S–X ) of the kidney glomerulus tissue ( A–L ) or the heart tissue ( M–X ) from a V30M FAP patient (67-year-old man) were prepared. After Congo red and hematoxylin staining ( C, I, O, and U ), the presence of apple-green birefringence under polarized light confirmed the presence of amyloid deposits ( D, J, P, and V ). For immunohistochemical staining, paraffin-embedded and frozen slides were treated with periodic acid, followed by immunostaining using T24 antibody ( A, G, M, and S ), anti-prealbumin antibody ( B, H, N, and T ), isotype control ( E, K, Q, and W ), or no primary antibody (secondary antibody only) ( F, L, R, and X ). Areas that are stained brown are deposits of TTR amyloids. Bars, 200 μm.

    Journal: The Journal of Biological Chemistry

    Article Title: Novel Antibody for the Treatment of Transthyretin Amyloidosis *

    doi: 10.1074/jbc.M116.738138

    Figure Lengend Snippet: T24 antibody recognized TTR amyloids in the kidney and heart from FAP patients. T24 reactivity was evaluated by immunohistochemical analysis. Paraffin sections ( A–F and M–R ) or frozen sections ( G–L and S–X ) of the kidney glomerulus tissue ( A–L ) or the heart tissue ( M–X ) from a V30M FAP patient (67-year-old man) were prepared. After Congo red and hematoxylin staining ( C, I, O, and U ), the presence of apple-green birefringence under polarized light confirmed the presence of amyloid deposits ( D, J, P, and V ). For immunohistochemical staining, paraffin-embedded and frozen slides were treated with periodic acid, followed by immunostaining using T24 antibody ( A, G, M, and S ), anti-prealbumin antibody ( B, H, N, and T ), isotype control ( E, K, Q, and W ), or no primary antibody (secondary antibody only) ( F, L, R, and X ). Areas that are stained brown are deposits of TTR amyloids. Bars, 200 μm.

    Article Snippet: In addition, analysis of the reactivity of T24 and polyclonal rabbit anti-human prealbumin (TTR) (Dako) was performed by Western blotting against the healthy patient's serum and extracted TTR amyloid from the FAP patient's kidney and heart.

    Techniques: Immunohistochemistry, Staining, Immunostaining

    RT24 promotes macrophage phagocytosis against aggregated TTR. A macrophage phagocytic ability test was performed to investigate the clearance via antibodies against misfolded TTR. Aggregated TTR were generated from native V30M TTR by incubating for 24 h at 37 °C under acidic conditions (pH 4.0). Aggregated TTR ( A ) or native TTR ( B ) or were co-cultured with iPS-MLs in medium containing antibodies (RT24, isotype control, or none, n = 3). After incubation for 2 days, reduction of TTR in the medium was evaluated by ELISA using anti-prealbumin antibody. Data are presented as mean ± S.D. Student's t test was used for evaluation. A p value less than 0.05 (*) was considered statistically significant.

    Journal: The Journal of Biological Chemistry

    Article Title: Novel Antibody for the Treatment of Transthyretin Amyloidosis *

    doi: 10.1074/jbc.M116.738138

    Figure Lengend Snippet: RT24 promotes macrophage phagocytosis against aggregated TTR. A macrophage phagocytic ability test was performed to investigate the clearance via antibodies against misfolded TTR. Aggregated TTR were generated from native V30M TTR by incubating for 24 h at 37 °C under acidic conditions (pH 4.0). Aggregated TTR ( A ) or native TTR ( B ) or were co-cultured with iPS-MLs in medium containing antibodies (RT24, isotype control, or none, n = 3). After incubation for 2 days, reduction of TTR in the medium was evaluated by ELISA using anti-prealbumin antibody. Data are presented as mean ± S.D. Student's t test was used for evaluation. A p value less than 0.05 (*) was considered statistically significant.

    Article Snippet: In addition, analysis of the reactivity of T24 and polyclonal rabbit anti-human prealbumin (TTR) (Dako) was performed by Western blotting against the healthy patient's serum and extracted TTR amyloid from the FAP patient's kidney and heart.

    Techniques: Generated, Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay

    T24 antibody specifically reacted with TTR amyloid, but not serum TTR, from FAP patients. A, T24 reactivity was assessed by ELISA. Human serum-derived WT-TTR fibril by acidic treatment ( black circle ), BSA-conjugated TTR peptide ( white circle ), serum from a healthy volunteer ( black square ), and serum from a V30M FAP patient ( white square ) were immobilized on an immunoplate, and the reactivity of T24 against each antigen was analyzed by ELISA. B and C, reactivity of T24 was assessed by Western blotting ( WB ). B, serum from a healthy volunteer or V30M FAP patient was applied to SDS-PAGE under non-reducing conditions and then analyzed by Western blotting with polyclonal anti-prealbumin or T24 antibody. C, serum or extracted amyloids from the heart and kidney from a V30M FAP patient were applied to SDS-PAGE, and then analyzed by Western blotting with T24 antibody.

    Journal: The Journal of Biological Chemistry

    Article Title: Novel Antibody for the Treatment of Transthyretin Amyloidosis *

    doi: 10.1074/jbc.M116.738138

    Figure Lengend Snippet: T24 antibody specifically reacted with TTR amyloid, but not serum TTR, from FAP patients. A, T24 reactivity was assessed by ELISA. Human serum-derived WT-TTR fibril by acidic treatment ( black circle ), BSA-conjugated TTR peptide ( white circle ), serum from a healthy volunteer ( black square ), and serum from a V30M FAP patient ( white square ) were immobilized on an immunoplate, and the reactivity of T24 against each antigen was analyzed by ELISA. B and C, reactivity of T24 was assessed by Western blotting ( WB ). B, serum from a healthy volunteer or V30M FAP patient was applied to SDS-PAGE under non-reducing conditions and then analyzed by Western blotting with polyclonal anti-prealbumin or T24 antibody. C, serum or extracted amyloids from the heart and kidney from a V30M FAP patient were applied to SDS-PAGE, and then analyzed by Western blotting with T24 antibody.

    Article Snippet: In addition, analysis of the reactivity of T24 and polyclonal rabbit anti-human prealbumin (TTR) (Dako) was performed by Western blotting against the healthy patient's serum and extracted TTR amyloid from the FAP patient's kidney and heart.

    Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Western Blot, SDS Page

    CP explants show typical CP markers. (A–E) Presence of transthyretin (TTR), aquaporin 1 (AQ1), ZO1, Claudin 3 and ICAM-1 with DAPI (blue) staining in CP explants. White arrows indicate the localization of the staining on the apical or basolateral cell membrane (bar 20 μm). Optical thickness of images sections 3 μm. (F) Distribution of F-actin (cyan) detected by phalloidin in ex vivo CP fragment and explants (G) . (H) E-cadherin staining in a CP explant. (I) Expression of the CP epithelial markers, Ttr , lipocalin-type prostaglandin D Synthase ( Ptgds ), transferrin ( Tf ), creatine kinase brain ( Ckb ), occludin ( Ocln ), insulin-like growth factor 2 ( IGF-2 ) and G protein-coupled receptor 125 (GPR125) in native mouse CP (white bars) and CP explant at day 12 (black bars). n = 3 for explant series and n = 4 for CP fragments.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Three-Dimensional Explant Platform for Studies on Choroid Plexus Epithelium

    doi: 10.3389/fncel.2020.00108

    Figure Lengend Snippet: CP explants show typical CP markers. (A–E) Presence of transthyretin (TTR), aquaporin 1 (AQ1), ZO1, Claudin 3 and ICAM-1 with DAPI (blue) staining in CP explants. White arrows indicate the localization of the staining on the apical or basolateral cell membrane (bar 20 μm). Optical thickness of images sections 3 μm. (F) Distribution of F-actin (cyan) detected by phalloidin in ex vivo CP fragment and explants (G) . (H) E-cadherin staining in a CP explant. (I) Expression of the CP epithelial markers, Ttr , lipocalin-type prostaglandin D Synthase ( Ptgds ), transferrin ( Tf ), creatine kinase brain ( Ckb ), occludin ( Ocln ), insulin-like growth factor 2 ( IGF-2 ) and G protein-coupled receptor 125 (GPR125) in native mouse CP (white bars) and CP explant at day 12 (black bars). n = 3 for explant series and n = 4 for CP fragments.

    Article Snippet: Anti-transthyretin (anti-TTR; Santa Cruz) was used at 1:500, anti-aquaporin 1 (AQ-1) and anti-ZO1 (both from Abcam) were used at 1:200 dilution.

    Techniques: Staining, Ex Vivo, Expressing

    Characteristics of the patients included in THAOS as of June 2013. a Five patients had gene polymorphisms and 40 patients had a non-biopsy proven diagnosis of wt-ATTR amyloidosis. b Thirty-two patients had an unknown symptomatic status. ATTR : transthyretin amyloid; n : number of subjects; THAOS : transthyretin amyloidosis outcomes survey; TTR : transthyretin; wt : wild-type.

    Journal: Orphanet Journal of Rare Diseases

    Article Title: THAOS: Gastrointestinal manifestations of transthyretin amyloidosis - common complications of a rare disease

    doi: 10.1186/1750-1172-9-61

    Figure Lengend Snippet: Characteristics of the patients included in THAOS as of June 2013. a Five patients had gene polymorphisms and 40 patients had a non-biopsy proven diagnosis of wt-ATTR amyloidosis. b Thirty-two patients had an unknown symptomatic status. ATTR : transthyretin amyloid; n : number of subjects; THAOS : transthyretin amyloidosis outcomes survey; TTR : transthyretin; wt : wild-type.

    Article Snippet: The Transthyretin Amyloidosis Outcomes Survey (THAOS) is the first global, multicenter, longitudinal, observational survey that collects data on patients with transthyretin amyloidosis and the registry is sponsored by Pfizer Inc.

    Techniques:

    Exogenous transthyretin rescues glomerular integrity and inhibits production of sFlt-1 and sEng in preeclamptic IL-10 −/− mice. A : Histopathological analysis of H E- or periodic acid schiff (PAS)-stained renal tissues from NPS −

    Journal: The American Journal of Pathology

    Article Title: Transthyretin Is Dysregulated in Preeclampsia, and Its Native Form Prevents the Onset of Disease in a Preclinical Mouse Model

    doi: 10.1016/j.ajpath.2013.07.022

    Figure Lengend Snippet: Exogenous transthyretin rescues glomerular integrity and inhibits production of sFlt-1 and sEng in preeclamptic IL-10 −/− mice. A : Histopathological analysis of H E- or periodic acid schiff (PAS)-stained renal tissues from NPS −

    Article Snippet: Human transthyretin in the mouse placenta was probed using transthyretin polyclonal rabbit antibody (1:200, overnight at 4°C; Dako) and detected using Cy-3–conjugated anti-rabbit IgG (1:100; Invitrogen).

    Techniques: Mouse Assay, Staining

    Immunoprecipitated (IP) transthyretin from preeclampsia serum (PES) induces disease features in IL-10 −/− mice. Pregnant mice were injected (i.p.) on gd 10 with transthyretin immunoprecipitate obtained from 100 μL normal pregnancy

    Journal: The American Journal of Pathology

    Article Title: Transthyretin Is Dysregulated in Preeclampsia, and Its Native Form Prevents the Onset of Disease in a Preclinical Mouse Model

    doi: 10.1016/j.ajpath.2013.07.022

    Figure Lengend Snippet: Immunoprecipitated (IP) transthyretin from preeclampsia serum (PES) induces disease features in IL-10 −/− mice. Pregnant mice were injected (i.p.) on gd 10 with transthyretin immunoprecipitate obtained from 100 μL normal pregnancy

    Article Snippet: Human transthyretin in the mouse placenta was probed using transthyretin polyclonal rabbit antibody (1:200, overnight at 4°C; Dako) and detected using Cy-3–conjugated anti-rabbit IgG (1:100; Invitrogen).

    Techniques: Immunoprecipitation, Mouse Assay, Injection

    Transthyretin in preeclampsia is dysregulated and forms aggregates. A : Analysis of serum transthyretin in normal pregnancy serum (NPS) and preeclampsia serum (PES) by ELISA. Transthyretin is present at significantly reduced levels in PES ( P

    Journal: The American Journal of Pathology

    Article Title: Transthyretin Is Dysregulated in Preeclampsia, and Its Native Form Prevents the Onset of Disease in a Preclinical Mouse Model

    doi: 10.1016/j.ajpath.2013.07.022

    Figure Lengend Snippet: Transthyretin in preeclampsia is dysregulated and forms aggregates. A : Analysis of serum transthyretin in normal pregnancy serum (NPS) and preeclampsia serum (PES) by ELISA. Transthyretin is present at significantly reduced levels in PES ( P

    Article Snippet: Human transthyretin in the mouse placenta was probed using transthyretin polyclonal rabbit antibody (1:200, overnight at 4°C; Dako) and detected using Cy-3–conjugated anti-rabbit IgG (1:100; Invitrogen).

    Techniques: Enzyme-linked Immunosorbent Assay

    Transthyretin binds to soluble endoglin and its possible mode of action. A : Far Western blot showing the binding of transthyretin with sEng and RBP-4 (red circle) and lack of binding to sFlt-1. Purified sFlt-1, sEng, and RBP-4 were used as prey proteins,

    Journal: The American Journal of Pathology

    Article Title: Transthyretin Is Dysregulated in Preeclampsia, and Its Native Form Prevents the Onset of Disease in a Preclinical Mouse Model

    doi: 10.1016/j.ajpath.2013.07.022

    Figure Lengend Snippet: Transthyretin binds to soluble endoglin and its possible mode of action. A : Far Western blot showing the binding of transthyretin with sEng and RBP-4 (red circle) and lack of binding to sFlt-1. Purified sFlt-1, sEng, and RBP-4 were used as prey proteins,

    Article Snippet: Human transthyretin in the mouse placenta was probed using transthyretin polyclonal rabbit antibody (1:200, overnight at 4°C; Dako) and detected using Cy-3–conjugated anti-rabbit IgG (1:100; Invitrogen).

    Techniques: Far Western Blot, Binding Assay, Purification

    Exogenous transthyretin inhibits preeclampsia-associated features in vitro and in vivo . A : Serum-induced and transthyretin-modified endovascular interaction between endothelial cells (human umbilical cord endothelial cells, cell tracker

    Journal: The American Journal of Pathology

    Article Title: Transthyretin Is Dysregulated in Preeclampsia, and Its Native Form Prevents the Onset of Disease in a Preclinical Mouse Model

    doi: 10.1016/j.ajpath.2013.07.022

    Figure Lengend Snippet: Exogenous transthyretin inhibits preeclampsia-associated features in vitro and in vivo . A : Serum-induced and transthyretin-modified endovascular interaction between endothelial cells (human umbilical cord endothelial cells, cell tracker

    Article Snippet: Human transthyretin in the mouse placenta was probed using transthyretin polyclonal rabbit antibody (1:200, overnight at 4°C; Dako) and detected using Cy-3–conjugated anti-rabbit IgG (1:100; Invitrogen).

    Techniques: In Vitro, In Vivo, Modification

    Surface enhanced laser desorption ionization-time-of-flight (SELDI-TOF) and biochemical analyses of transthyretin in preeclampsia serum. A : Preeclampsia serum (PES) ( n = 53) and normal pregnancy serum (NPS) ( n = 16) were analyzed by SELDI-TOF in the molecular

    Journal: The American Journal of Pathology

    Article Title: Transthyretin Is Dysregulated in Preeclampsia, and Its Native Form Prevents the Onset of Disease in a Preclinical Mouse Model

    doi: 10.1016/j.ajpath.2013.07.022

    Figure Lengend Snippet: Surface enhanced laser desorption ionization-time-of-flight (SELDI-TOF) and biochemical analyses of transthyretin in preeclampsia serum. A : Preeclampsia serum (PES) ( n = 53) and normal pregnancy serum (NPS) ( n = 16) were analyzed by SELDI-TOF in the molecular

    Article Snippet: Human transthyretin in the mouse placenta was probed using transthyretin polyclonal rabbit antibody (1:200, overnight at 4°C; Dako) and detected using Cy-3–conjugated anti-rabbit IgG (1:100; Invitrogen).

    Techniques:

    Transthyretin-immunodepleted preeclampsia serum (PES) fails to induce preeclampsia-like features. Transthyretin-depleted PES (100 μL) was injected on gd10 in IL-10 −/− mice, and its effects on fetal size, blood pressure, proteinuria,

    Journal: The American Journal of Pathology

    Article Title: Transthyretin Is Dysregulated in Preeclampsia, and Its Native Form Prevents the Onset of Disease in a Preclinical Mouse Model

    doi: 10.1016/j.ajpath.2013.07.022

    Figure Lengend Snippet: Transthyretin-immunodepleted preeclampsia serum (PES) fails to induce preeclampsia-like features. Transthyretin-depleted PES (100 μL) was injected on gd10 in IL-10 −/− mice, and its effects on fetal size, blood pressure, proteinuria,

    Article Snippet: Human transthyretin in the mouse placenta was probed using transthyretin polyclonal rabbit antibody (1:200, overnight at 4°C; Dako) and detected using Cy-3–conjugated anti-rabbit IgG (1:100; Invitrogen).

    Techniques: Injection, Mouse Assay