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  • 96
    JEOL transmission electron microscope
    Representative <t>TEM</t> images tetragonal BaTiO 3 <t>nanoparticles.</t> ( A,B ) TEM images of tetragonal BaTiO 3 nanoparticles after the removal of carbon shell layer with different scale bars. ( C ) TEM image of tetragonal BaTiO 3 nanoparticles capped by bi-functional ligands. ( D ) HR-TEM of tetragonal BaTiO 3 nanocrystals coated with bi-functional ligands. The digital image of tetragonal BaTiO 3 nanoparticles without carbon layer as the inset in ( A ). The digital image of toluene solution of tetragonal BaTiO 3 nanocrystals coated with bi-functional ligands as the inset in ( C ).
    Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 96/100, based on 21371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Hitachi Ltd transmission electron microscope
    Representative <t>TEM</t> images tetragonal BaTiO 3 <t>nanoparticles.</t> ( A,B ) TEM images of tetragonal BaTiO 3 nanoparticles after the removal of carbon shell layer with different scale bars. ( C ) TEM image of tetragonal BaTiO 3 nanoparticles capped by bi-functional ligands. ( D ) HR-TEM of tetragonal BaTiO 3 nanocrystals coated with bi-functional ligands. The digital image of tetragonal BaTiO 3 nanoparticles without carbon layer as the inset in ( A ). The digital image of toluene solution of tetragonal BaTiO 3 nanocrystals coated with bi-functional ligands as the inset in ( C ).
    Transmission Electron Microscope, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 92/100, based on 10725 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    JEOL transmission electron microscopy tem
    (A) Picture showing 0.1% <t>CsA-loaded</t> NMF ( right ) in comparison to water ( left ). (B) <t>TEM</t> image for 0.1% CsA-loaded NMF ( scale : 500 nm).
    Transmission Electron Microscopy Tem, supplied by JEOL, used in various techniques. Bioz Stars score: 95/100, based on 13489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Philips Healthcare transmission electron microscope
    (A) Picture showing 0.1% <t>CsA-loaded</t> NMF ( right ) in comparison to water ( left ). (B) <t>TEM</t> image for 0.1% CsA-loaded NMF ( scale : 500 nm).
    Transmission Electron Microscope, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 6254 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    JEOL jem 1400 transmission electron microscope
    A super-low dose of LPS induces mitochondrial fission in murine macrophages. A , WT BMDMs were treated with a super-low dose of LPS (50 pg/ml) for 1 h and then labeled with MitoTracker Red to stain the mitochondria. The nuclei were stained using DAPI ( blue ). The cells were visualized under a Zeiss LSM510 laser-scanning confocal microscope (original magnification ×400). The merged images were magnified and are shown at the right. B , WT BMDMs were treated with 50 pg/ml LPS for 1 h. Cells were prepared and visualized under a JEOL <t>JEM</t> 1400 transmission electron microscope. The arrows denote fragmented mitochondria.
    Jem 1400 Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 94/100, based on 3736 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Carl Zeiss transmission electron microscope
    A super-low dose of LPS induces mitochondrial fission in murine macrophages. A , WT BMDMs were treated with a super-low dose of LPS (50 pg/ml) for 1 h and then labeled with MitoTracker Red to stain the mitochondria. The nuclei were stained using DAPI ( blue ). The cells were visualized under a Zeiss LSM510 laser-scanning confocal microscope (original magnification ×400). The merged images were magnified and are shown at the right. B , WT BMDMs were treated with 50 pg/ml LPS for 1 h. Cells were prepared and visualized under a JEOL <t>JEM</t> 1400 transmission electron microscope. The arrows denote fragmented mitochondria.
    Transmission Electron Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 3031 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    JEOL jem 1010 transmission electron microscope
    Morphology of platelet and MK cells from the proband and a control. ( A ) Morphology of platelets in the bone marrow smears, which were stained with May-Grunwald Giemsa. The platelet in the proband is much larger than its normal size by reference to the red blood cells and to the control. The analysis was performed on an optical microscope (Leica DMR) with a 50×/1.40 numerical aperture oil objective lens (Leica). ( B ) Morphology and size (indicated by red arrows in the proband) of platelets from the peripheral blood observed by electronic microscopy (magnification of 10000×). ( C ) Morphology of a representative cultured MK from the bone marrow observed by electronic microscopy (magnification of 6000×). Ultrathin sections were examined by a <t>JEM-1010</t> transmission electron microscope (JEOL) at an accelerating voltage of 80 kV.
    Jem 1010 Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 94/100, based on 3129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    JEOL jem 1230 transmission electron microscope
    Phagosomal escape of F. tularensis . J774 cells were infected with F. tularensis at an MOI of 1,000 for 2 h and, after washing, incubated for another 6 h before they were fixed and analyzed by transmission electron microscopy (TEM). (A) Electron micrographs of infected J774 cells were acquired with a JEOL <t>JEM</t> 1230 Transmission Electron Microscope (JEOL Ltd., Tokyo, Japan). Black arrows indicate vacuolar membranes surrounding intracellular bacteria. (B) Bacteria were divided into one of 4 categories based on the membrane integrity of the surrounding vacuolar membrane. Micrographs in (A) illustrate the categories “Cytoplasm” (LVS and Δ iglE/E ) or “Intact phagosome” (Δ iglE and Δ iglC ).
    Jem 1230 Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 94/100, based on 3184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hitachi Ltd h 7650 transmission electron microscope
    Transmission electron micrograph of phage P545 (A) , P539 (B) , and P507 (C) . The phage filtrate was applied to a copper grid before negative staining with phosphotungstic acid (PTA, 2% w/v). Electron micrographs were observed using an <t>H_7650</t> (Hitachi, Tokyo, Japan) TEM.
    H 7650 Transmission Electron Microscope, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 94/100, based on 3051 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    JEOL 1200ex transmission electron microscope
    Ultrastructural analysis of endothelial cells infiltrating the exogenous tumors within WT and Cav-1 KO mice. Exogenous tumors were fixed with 2.5% glutaraldehyde in 0.1 mol/L of cacodylate buffer, postfixed with OsO4, and stained with uranyl acetate and lead citrate. Samples were examined under a JEOL <t>1200EX</t> transmission electron microscope. A: Caveolar morphology. High-magnification electron micrographs of nascent capillaries formed within exogenous tumors are shown. Note that in WT endothelial cells, caveolae organelles (50- to 100-nm vesicles and invaginations) are particularly abundant ( arrows ); in contrast, in Cav-1 KO endothelial cells, caveolae formation is completely ablated. B and C: Nascent capillary formation. Low-magnification electron micrographs of nascent capillaries formed within exogenous tumors are shown. Note that nascent capillary formation is incomplete and disorganized in tumors grown in Cav-1 KO mice. B shows larger nascent capillaries, while C shows examples of smaller nascent capillaries. Endo, endothelial cell; RBC, red blood cell; Nuc, nucleus. Scale bars: 400 nm ( A ); 2 μm ( B and C ).
    1200ex Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 94/100, based on 3157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    JEOL 1010 transmission electron microscope
    Transmission electron microscopy (TEM) of human lung TLA TEM image of a human lung TLA at 50000x (A–C) and 12000x (D). A) The highly integrated nature of the uninfected TLA with healthy cell layers from the microcarrier to the external cell surface. Cell nuclei (N) are indicated. B) Infected lung TL with RSV nucleocapsids in the peri-nuclear region of the cells (VNC). C–D) Budding RSV virions (BV) at the surface of lung TLA. Images were taken using a JEOL-JEM <t>1010</t> transmission electron microscope.
    1010 Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 94/100, based on 2343 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    JEOL jem 1011 transmission electron microscope
    P38 inhibitor abolished GFP-PMCA4b internalization in A375 cells, and chloroquine (CQ) trapped the pump in LAMP1 positive intracellular organelles. ( A ) Confocal microscopy images of A375-GFP-PMCA4b cells after treatment with 10 µM p38i-2 inhibitor (SB202190) for 48 h with or without starvation (3 h). The cells were immunostained for the early endosomal marker EEA1. EEA1 and GFP-PMCA4b positive (yellow) vesicles were counted (six cells/group) and expressed as % of total number of GFP-PMCA4b positive vesicles. Scale bar, 20 µm. Bars represent means ± SE. ( B ) Confocal images of A375-GFP-PMCA4b immunostained with the endo-lysosomal markers Rab7, Rab11 and LAMP1, and the autophagy marker LC3 after treatment with CQ for 3 h. The number of GFP-PMCA4b vesicles that co-localize with the endo-lysosomal or autophagy markers were counted and expressed as a % of total number of PMCA4b positive vesicles. Seven to nine cells were counted in each group. Scale bar, 20 µm. Bars represent means ± SE. ( A , B ) Insets show higher magnification view, Scale bar, 10 µM. Arrowheads show co-localization of GFP-PMCA4b with endosomal markers. ( C ) A375-GFP-PMCA4b cells were cultured and treated with CQ for 3 h. Cells were fixed and immunostained with anti-GFP antibody (1:100) (Invitrogen, 1972783). Images were taken by <t>JEM-1011</t> transmission electron microscope (Jeol). Arrowheads show positive PMCA4b protein ( D ) A375 cells were treated with the proteasome inhibitor MG132 for 6 h at the concentrations indicated. Protein expression was analyzed by Western blotting. The experiment was repeated three times. ( E ) Schematic diagram shows the pathway of PMCA4b degradation through the endo-lysosomal system.
    Jem 1011 Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 94/100, based on 2642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Philips Healthcare cm10 transmission electron microscope
    Electron microscopy images of chloroplasts from wild type (WT) and the RNAi-W1-7 plants (W1-7) . Leaf segments (2 × 2 mm) from 10 days old primary foliage leaves were taken at a position of 2 cm below the leaf tip. Some of the DNA containing regions are indicated by arrows. Transmission electron microscopy was performed with a Philips <t>CM10</t> transmission electron microscope. Bars represent 500 nm.
    Cm10 Transmission Electron Microscope, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 2351 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Hitachi Ltd transmission electron microscopy tem
    Physicochemical characterization of <t>VCR-loaded</t> T7-LDL. (A) Particle size distribution of VCR-loaded T7-LDL. (B) Morphological appearance of VCR-loaded T7-LDL based on <t>TEM.</t> Stability of VCR-loaded GKRK-APO in the full rat serum. (C) The transmission and backscattering profiles were measured at each time point using a Turbiscan Lab ® Expert analyzer. (D) In vitro release of VCR from T7-LDL and LDL at pH 7.4 at 37 °C, respectively. The data are presented as the means ± SD ( n = 3). *Indicates p
    Transmission Electron Microscopy Tem, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 94/100, based on 2377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Philips Healthcare cm100 transmission electron microscope
    PrD-polyQ fusion proteins form seeding-competent amyloid structures in vitro . (A) Schematic representation of MBP fusion proteins with polyQ tracts of different lengths. (B) Time-resolved analysis of polyQ-mediated aggregation of PrD fusions by FRA. SDS-resistant protein aggregates retained on filter membranes were detected using an anti-Sup35 antibody. (C) Electron micrographs of polyQ-containing PrD protein aggregates. MBP fusions were digested with factor Xa without agitation at 37°C for 24h, stained with 1% uranyl acetate and visualised using a Philips <t>CM100</t> transmission electron microscope. (D) Schematic description of an in vitro seeding assay. The formation of seeding-competent polyQ-containing PrD protein aggregates after cleavage of MBP fusion proteins with factor Xa and the subsequent conversion of HisSup35 protein from the soluble to the amyloidogenic state is shown. SDS-stable protein aggregates were monitored by FRA using specific antibodies. (E) Analysis of HisSup35 aggregate formation by FRA. MBP-PrD-polyQ fusion proteins together with MBP-HisSup35 or MBP-HisCT were incubated with factor Xa at 37°C for 24 and 48 h. Aggregates retained on filter membranes were detected using anti-His and anti-Sup35 antibodies.
    Cm100 Transmission Electron Microscope, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 2102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher transmission electron microscope
    PrD-polyQ fusion proteins form seeding-competent amyloid structures in vitro . (A) Schematic representation of MBP fusion proteins with polyQ tracts of different lengths. (B) Time-resolved analysis of polyQ-mediated aggregation of PrD fusions by FRA. SDS-resistant protein aggregates retained on filter membranes were detected using an anti-Sup35 antibody. (C) Electron micrographs of polyQ-containing PrD protein aggregates. MBP fusions were digested with factor Xa without agitation at 37°C for 24h, stained with 1% uranyl acetate and visualised using a Philips <t>CM100</t> transmission electron microscope. (D) Schematic description of an in vitro seeding assay. The formation of seeding-competent polyQ-containing PrD protein aggregates after cleavage of MBP fusion proteins with factor Xa and the subsequent conversion of HisSup35 protein from the soluble to the amyloidogenic state is shown. SDS-stable protein aggregates were monitored by FRA using specific antibodies. (E) Analysis of HisSup35 aggregate formation by FRA. MBP-PrD-polyQ fusion proteins together with MBP-HisSup35 or MBP-HisCT were incubated with factor Xa at 37°C for 24 and 48 h. Aggregates retained on filter membranes were detected using anti-His and anti-Sup35 antibodies.
    Transmission Electron Microscope, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1814 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    JEOL 1400 transmission electron microscope
    Epithelial- and mesenchymal-type MCAs differ in cross-sectional ultrastructure. Representative epithelial (A-B, OvCa433) and mesenchymal (C-D, Dov13) MCAs were generated via the hanging drop method (Supplemental Figure 1A), observed under light microscope at 4× magnification (A and C), and further processed for TEM as detailed in Methods. Sections were examined under JEOL <t>1400</t> TEM (B and D). Scale bar: 10 μm.
    1400 Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 92/100, based on 1704 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Philips Healthcare transmission electron microscopy tem
    (A) HRTEM image (inset: <t>TEM</t> image of low magnification); (B) <t>SAED</t> pattern; (C) FTT images; (D) Line-scan EDS; and (E) EDS analysis of Pb 43 Te 56 hollow nanofibers. The electrolyte consisted of 50 mM Pb 2+ , 0.1 mM HTeO 2 + , and 0.1 M HNO 3 at room temperature.
    Transmission Electron Microscopy Tem, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 1857 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    JEOL jem 2100 transmission electron microscope
    Transmission electron microscope (TEM) images of density gradient purified AHSV-1 VLPs ( a ), AHSV-1 CLPs ( b ), chimeric AHSV-1/AHSV-7 VLPs ( c ), double chimeric AHSV-1/AHSV-7 VLPs ( d ) and triple chimeric AHSV-1/AHSV-6/AHSV-3 VLPs ( e - f ). Particles were visualized with a <t>JEM-2100</t> Transmission electron microscope (JEOL). Indicated with red arrows are the virus-like particles (VLPs) and indicated with the blue arrow is a core-like particle (CLP)
    Jem 2100 Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 93/100, based on 1558 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Representative TEM images tetragonal BaTiO 3 nanoparticles. ( A,B ) TEM images of tetragonal BaTiO 3 nanoparticles after the removal of carbon shell layer with different scale bars. ( C ) TEM image of tetragonal BaTiO 3 nanoparticles capped by bi-functional ligands. ( D ) HR-TEM of tetragonal BaTiO 3 nanocrystals coated with bi-functional ligands. The digital image of tetragonal BaTiO 3 nanoparticles without carbon layer as the inset in ( A ). The digital image of toluene solution of tetragonal BaTiO 3 nanocrystals coated with bi-functional ligands as the inset in ( C ).

    Journal: Scientific Reports

    Article Title: Facile Fabrication of Size-Tunable Core/Shell Ferroelectric/Polymeric Nanoparticles with Tailorable Dielectric Properties via Organocatalyzed Atom Transfer Radical Polymerization Driven by Visible Light

    doi: 10.1038/s41598-018-38039-8

    Figure Lengend Snippet: Representative TEM images tetragonal BaTiO 3 nanoparticles. ( A,B ) TEM images of tetragonal BaTiO 3 nanoparticles after the removal of carbon shell layer with different scale bars. ( C ) TEM image of tetragonal BaTiO 3 nanoparticles capped by bi-functional ligands. ( D ) HR-TEM of tetragonal BaTiO 3 nanocrystals coated with bi-functional ligands. The digital image of tetragonal BaTiO 3 nanoparticles without carbon layer as the inset in ( A ). The digital image of toluene solution of tetragonal BaTiO 3 nanocrystals coated with bi-functional ligands as the inset in ( C ).

    Article Snippet: Shapes and dimensions of cubic BaTiO3 /HBPA nanocomposites, carbon-capped tetragonal BaTiO3 nanoparticles, tetragonal BaTiO3 nanoparticles after removal of carbon shell, tetragonal BaTiO3 nanoparticles coated with the metal-free ATRP initiators and core/shell ferroelectric BaTiO3 /PMMA hybrid nanoparticles, were observed via transmission electron microscope (TEM) (JEOL 1200EX; characterized at 80 kV).

    Techniques: Transmission Electron Microscopy, Functional Assay

    TEM images of cubic BaTiO 3 nanoparticles embedded in HBPA matrix with different scale bars. The inset in ( B ) shows the digital image of BaTiO 3 /HBPA sample.

    Journal: Scientific Reports

    Article Title: Facile Fabrication of Size-Tunable Core/Shell Ferroelectric/Polymeric Nanoparticles with Tailorable Dielectric Properties via Organocatalyzed Atom Transfer Radical Polymerization Driven by Visible Light

    doi: 10.1038/s41598-018-38039-8

    Figure Lengend Snippet: TEM images of cubic BaTiO 3 nanoparticles embedded in HBPA matrix with different scale bars. The inset in ( B ) shows the digital image of BaTiO 3 /HBPA sample.

    Article Snippet: Shapes and dimensions of cubic BaTiO3 /HBPA nanocomposites, carbon-capped tetragonal BaTiO3 nanoparticles, tetragonal BaTiO3 nanoparticles after removal of carbon shell, tetragonal BaTiO3 nanoparticles coated with the metal-free ATRP initiators and core/shell ferroelectric BaTiO3 /PMMA hybrid nanoparticles, were observed via transmission electron microscope (TEM) (JEOL 1200EX; characterized at 80 kV).

    Techniques: Transmission Electron Microscopy

    Representative TEM images of tetragonal BaTiO 3 after high temperature calcinations. ( A , B,C ) TEM images of tetragonal BaTiO 3 nanocrystals coated with carbon layer. ( D ) HR-TEM image of tetragonal BaTiO 3 nanoparticles capped with carbon layer. The digital image of tetragonal BaTiO 3 nanoparticles capped with carbon layer as the inset in ( B ).

    Journal: Scientific Reports

    Article Title: Facile Fabrication of Size-Tunable Core/Shell Ferroelectric/Polymeric Nanoparticles with Tailorable Dielectric Properties via Organocatalyzed Atom Transfer Radical Polymerization Driven by Visible Light

    doi: 10.1038/s41598-018-38039-8

    Figure Lengend Snippet: Representative TEM images of tetragonal BaTiO 3 after high temperature calcinations. ( A , B,C ) TEM images of tetragonal BaTiO 3 nanocrystals coated with carbon layer. ( D ) HR-TEM image of tetragonal BaTiO 3 nanoparticles capped with carbon layer. The digital image of tetragonal BaTiO 3 nanoparticles capped with carbon layer as the inset in ( B ).

    Article Snippet: Shapes and dimensions of cubic BaTiO3 /HBPA nanocomposites, carbon-capped tetragonal BaTiO3 nanoparticles, tetragonal BaTiO3 nanoparticles after removal of carbon shell, tetragonal BaTiO3 nanoparticles coated with the metal-free ATRP initiators and core/shell ferroelectric BaTiO3 /PMMA hybrid nanoparticles, were observed via transmission electron microscope (TEM) (JEOL 1200EX; characterized at 80 kV).

    Techniques: Transmission Electron Microscopy

    TEM characterization of core/shell tetragonal BaTiO 3 /PMMA nanoparticles with different scale bars. ( A ) TEM image of core/shell tetragonal BaTiO 3 /PMMA nanoparticles; Inset: digital image of toluene solution of tetragonal BaTiO 3 /PMMA nanoparticles. ( B ) TEM image of core/shell tetragonal BaTiO 3 /PMMA nanoparticles after PMMA macromolecular shell stained by RuO 4 .

    Journal: Scientific Reports

    Article Title: Facile Fabrication of Size-Tunable Core/Shell Ferroelectric/Polymeric Nanoparticles with Tailorable Dielectric Properties via Organocatalyzed Atom Transfer Radical Polymerization Driven by Visible Light

    doi: 10.1038/s41598-018-38039-8

    Figure Lengend Snippet: TEM characterization of core/shell tetragonal BaTiO 3 /PMMA nanoparticles with different scale bars. ( A ) TEM image of core/shell tetragonal BaTiO 3 /PMMA nanoparticles; Inset: digital image of toluene solution of tetragonal BaTiO 3 /PMMA nanoparticles. ( B ) TEM image of core/shell tetragonal BaTiO 3 /PMMA nanoparticles after PMMA macromolecular shell stained by RuO 4 .

    Article Snippet: Shapes and dimensions of cubic BaTiO3 /HBPA nanocomposites, carbon-capped tetragonal BaTiO3 nanoparticles, tetragonal BaTiO3 nanoparticles after removal of carbon shell, tetragonal BaTiO3 nanoparticles coated with the metal-free ATRP initiators and core/shell ferroelectric BaTiO3 /PMMA hybrid nanoparticles, were observed via transmission electron microscope (TEM) (JEOL 1200EX; characterized at 80 kV).

    Techniques: Transmission Electron Microscopy, Staining

    (A) Picture showing 0.1% CsA-loaded NMF ( right ) in comparison to water ( left ). (B) TEM image for 0.1% CsA-loaded NMF ( scale : 500 nm).

    Journal: Translational Vision Science & Technology

    Article Title: Topical, Aqueous, Clear Cyclosporine Formulation Design for Anterior and Posterior Ocular Delivery

    doi: 10.1167/tvst.4.3.1

    Figure Lengend Snippet: (A) Picture showing 0.1% CsA-loaded NMF ( right ) in comparison to water ( left ). (B) TEM image for 0.1% CsA-loaded NMF ( scale : 500 nm).

    Article Snippet: Shapes and surface morphology of CsA-loaded NMF were determined with transmission electron microscopy (TEM) (JEOL JEM 1200 EX II Electron Microscope; Peabody, MA) using negative staining with uranyl acetate (UA).

    Techniques: Transmission Electron Microscopy

    FESEM images of (A) CY4, (B) CY7, (C) CY10, and (D) CY13 and (E) TEM images of CY4 (inset: lattice spacing).

    Journal: PLoS ONE

    Article Title: Effects of Electrodeposition Mode and Deposition Cycle on the Electrochemical Performance of MnO2-NiO Composite Electrodes for High-Energy-Density Supercapacitors

    doi: 10.1371/journal.pone.0154566

    Figure Lengend Snippet: FESEM images of (A) CY4, (B) CY7, (C) CY10, and (D) CY13 and (E) TEM images of CY4 (inset: lattice spacing).

    Article Snippet: Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) images of the electrodes were captured using Jeol JSM-7600F and Jeol JEM 2100F instruments.

    Techniques: Transmission Electron Microscopy

    FESEM (left) and TEM (right) images of (A) CP, (B) CA, and (C) CY7 electrodes.

    Journal: PLoS ONE

    Article Title: Effects of Electrodeposition Mode and Deposition Cycle on the Electrochemical Performance of MnO2-NiO Composite Electrodes for High-Energy-Density Supercapacitors

    doi: 10.1371/journal.pone.0154566

    Figure Lengend Snippet: FESEM (left) and TEM (right) images of (A) CP, (B) CA, and (C) CY7 electrodes.

    Article Snippet: Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) images of the electrodes were captured using Jeol JSM-7600F and Jeol JEM 2100F instruments.

    Techniques: Transmission Electron Microscopy

    ( A ) UV–Vis spectroscopy of supernatant from CCNF, CCNF-DA and CCNF films. The insect in ( A ) shows the supernatant from the CCNF-DA/AgNPs film; ( B ) high-resolution transmission electron microscopy (HRTEM) image of extracted AgNPs. The inset black box in ( B ) indicates the area where the enlarged HRTEM image (bottom-right panel) was taken; ( C ) selected area electron diffraction (SAED) pattern of silver crystal of CCNF-DA/AgNP.

    Journal: Polymers

    Article Title: Mussel-Inspired Anisotropic Nanocellulose and Silver Nanoparticle Composite with Improved Mechanical Properties, Electrical Conductivity and Antibacterial Activity

    doi: 10.3390/polym8030102

    Figure Lengend Snippet: ( A ) UV–Vis spectroscopy of supernatant from CCNF, CCNF-DA and CCNF films. The insect in ( A ) shows the supernatant from the CCNF-DA/AgNPs film; ( B ) high-resolution transmission electron microscopy (HRTEM) image of extracted AgNPs. The inset black box in ( B ) indicates the area where the enlarged HRTEM image (bottom-right panel) was taken; ( C ) selected area electron diffraction (SAED) pattern of silver crystal of CCNF-DA/AgNP.

    Article Snippet: TEM images, selected area electron diffraction (SAED) pattern and energy-dispersive X-ray spectroscopy (EDS) data were obtained through high-resolution transmission electron microscopy (HRTEM; JEOL JEM-2100F, Tokyo, Japan).

    Techniques: UV-Vis Spectroscopy, Transmission Assay, Electron Microscopy

    A super-low dose of LPS induces mitochondrial fission in murine macrophages. A , WT BMDMs were treated with a super-low dose of LPS (50 pg/ml) for 1 h and then labeled with MitoTracker Red to stain the mitochondria. The nuclei were stained using DAPI ( blue ). The cells were visualized under a Zeiss LSM510 laser-scanning confocal microscope (original magnification ×400). The merged images were magnified and are shown at the right. B , WT BMDMs were treated with 50 pg/ml LPS for 1 h. Cells were prepared and visualized under a JEOL JEM 1400 transmission electron microscope. The arrows denote fragmented mitochondria.

    Journal: The Journal of Biological Chemistry

    Article Title: Molecular and Cellular Mechanisms Responsible for Cellular Stress and Low-grade Inflammation Induced by a Super-low Dose of Endotoxin *

    doi: 10.1074/jbc.M114.569210

    Figure Lengend Snippet: A super-low dose of LPS induces mitochondrial fission in murine macrophages. A , WT BMDMs were treated with a super-low dose of LPS (50 pg/ml) for 1 h and then labeled with MitoTracker Red to stain the mitochondria. The nuclei were stained using DAPI ( blue ). The cells were visualized under a Zeiss LSM510 laser-scanning confocal microscope (original magnification ×400). The merged images were magnified and are shown at the right. B , WT BMDMs were treated with 50 pg/ml LPS for 1 h. Cells were prepared and visualized under a JEOL JEM 1400 transmission electron microscope. The arrows denote fragmented mitochondria.

    Article Snippet: Samples were sliced and prepared on grids for visualization on a JEOL JEM 1400 transmission electron microscope.

    Techniques: Labeling, Staining, Microscopy, Transmission Assay

    Morphology of platelet and MK cells from the proband and a control. ( A ) Morphology of platelets in the bone marrow smears, which were stained with May-Grunwald Giemsa. The platelet in the proband is much larger than its normal size by reference to the red blood cells and to the control. The analysis was performed on an optical microscope (Leica DMR) with a 50×/1.40 numerical aperture oil objective lens (Leica). ( B ) Morphology and size (indicated by red arrows in the proband) of platelets from the peripheral blood observed by electronic microscopy (magnification of 10000×). ( C ) Morphology of a representative cultured MK from the bone marrow observed by electronic microscopy (magnification of 6000×). Ultrathin sections were examined by a JEM-1010 transmission electron microscope (JEOL) at an accelerating voltage of 80 kV.

    Journal: PLoS ONE

    Article Title: A Missense Mutation in the Alpha-Actinin 1 Gene (ACTN1) Is the Cause of Autosomal Dominant Macrothrombocytopenia in a Large French Family

    doi: 10.1371/journal.pone.0074728

    Figure Lengend Snippet: Morphology of platelet and MK cells from the proband and a control. ( A ) Morphology of platelets in the bone marrow smears, which were stained with May-Grunwald Giemsa. The platelet in the proband is much larger than its normal size by reference to the red blood cells and to the control. The analysis was performed on an optical microscope (Leica DMR) with a 50×/1.40 numerical aperture oil objective lens (Leica). ( B ) Morphology and size (indicated by red arrows in the proband) of platelets from the peripheral blood observed by electronic microscopy (magnification of 10000×). ( C ) Morphology of a representative cultured MK from the bone marrow observed by electronic microscopy (magnification of 6000×). Ultrathin sections were examined by a JEM-1010 transmission electron microscope (JEOL) at an accelerating voltage of 80 kV.

    Article Snippet: The samples were then processed for electron microscopy according to standard procedures, and sections were analyzed using a JEOL JEM-1010 transmission electron microscope.

    Techniques: Staining, Microscopy, Cell Culture, Transmission Assay

    Phagosomal escape of F. tularensis . J774 cells were infected with F. tularensis at an MOI of 1,000 for 2 h and, after washing, incubated for another 6 h before they were fixed and analyzed by transmission electron microscopy (TEM). (A) Electron micrographs of infected J774 cells were acquired with a JEOL JEM 1230 Transmission Electron Microscope (JEOL Ltd., Tokyo, Japan). Black arrows indicate vacuolar membranes surrounding intracellular bacteria. (B) Bacteria were divided into one of 4 categories based on the membrane integrity of the surrounding vacuolar membrane. Micrographs in (A) illustrate the categories “Cytoplasm” (LVS and Δ iglE/E ) or “Intact phagosome” (Δ iglE and Δ iglC ).

    Journal: Virulence

    Article Title: A mutagenesis-based approach identifies amino acids in the N-terminal part of Francisella tularensis IglE that critically control Type VI system-mediated secretion

    doi: 10.1080/21505594.2016.1258507

    Figure Lengend Snippet: Phagosomal escape of F. tularensis . J774 cells were infected with F. tularensis at an MOI of 1,000 for 2 h and, after washing, incubated for another 6 h before they were fixed and analyzed by transmission electron microscopy (TEM). (A) Electron micrographs of infected J774 cells were acquired with a JEOL JEM 1230 Transmission Electron Microscope (JEOL Ltd., Tokyo, Japan). Black arrows indicate vacuolar membranes surrounding intracellular bacteria. (B) Bacteria were divided into one of 4 categories based on the membrane integrity of the surrounding vacuolar membrane. Micrographs in (A) illustrate the categories “Cytoplasm” (LVS and Δ iglE/E ) or “Intact phagosome” (Δ iglE and Δ iglC ).

    Article Snippet: Sections were viewed with a JEOL JEM 1230 Transmission Electron Microscope (JEOL Ltd., Tokyo, Japan).

    Techniques: Infection, Incubation, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy, Microscopy

    Transmission electron micrograph of phage P545 (A) , P539 (B) , and P507 (C) . The phage filtrate was applied to a copper grid before negative staining with phosphotungstic acid (PTA, 2% w/v). Electron micrographs were observed using an H_7650 (Hitachi, Tokyo, Japan) TEM.

    Journal: Frontiers in Microbiology

    Article Title: Isolation and Characterization of Novel Lytic Bacteriophages Infecting Epidemic Carbapenem-Resistant Klebsiella pneumoniae Strains

    doi: 10.3389/fmicb.2020.01554

    Figure Lengend Snippet: Transmission electron micrograph of phage P545 (A) , P539 (B) , and P507 (C) . The phage filtrate was applied to a copper grid before negative staining with phosphotungstic acid (PTA, 2% w/v). Electron micrographs were observed using an H_7650 (Hitachi, Tokyo, Japan) TEM.

    Article Snippet: Electron micrographs were observed using an H_7650 transmission electron microscope (Hitachi, Tokyo, Japan).

    Techniques: Transmission Assay, Negative Staining, Transmission Electron Microscopy

    Ultrastructural analysis of endothelial cells infiltrating the exogenous tumors within WT and Cav-1 KO mice. Exogenous tumors were fixed with 2.5% glutaraldehyde in 0.1 mol/L of cacodylate buffer, postfixed with OsO4, and stained with uranyl acetate and lead citrate. Samples were examined under a JEOL 1200EX transmission electron microscope. A: Caveolar morphology. High-magnification electron micrographs of nascent capillaries formed within exogenous tumors are shown. Note that in WT endothelial cells, caveolae organelles (50- to 100-nm vesicles and invaginations) are particularly abundant ( arrows ); in contrast, in Cav-1 KO endothelial cells, caveolae formation is completely ablated. B and C: Nascent capillary formation. Low-magnification electron micrographs of nascent capillaries formed within exogenous tumors are shown. Note that nascent capillary formation is incomplete and disorganized in tumors grown in Cav-1 KO mice. B shows larger nascent capillaries, while C shows examples of smaller nascent capillaries. Endo, endothelial cell; RBC, red blood cell; Nuc, nucleus. Scale bars: 400 nm ( A ); 2 μm ( B and C ).

    Journal: The American Journal of Pathology

    Article Title: Caveolin-1 Knockout Mice Show an Impaired Angiogenic Response to Exogenous Stimuli

    doi:

    Figure Lengend Snippet: Ultrastructural analysis of endothelial cells infiltrating the exogenous tumors within WT and Cav-1 KO mice. Exogenous tumors were fixed with 2.5% glutaraldehyde in 0.1 mol/L of cacodylate buffer, postfixed with OsO4, and stained with uranyl acetate and lead citrate. Samples were examined under a JEOL 1200EX transmission electron microscope. A: Caveolar morphology. High-magnification electron micrographs of nascent capillaries formed within exogenous tumors are shown. Note that in WT endothelial cells, caveolae organelles (50- to 100-nm vesicles and invaginations) are particularly abundant ( arrows ); in contrast, in Cav-1 KO endothelial cells, caveolae formation is completely ablated. B and C: Nascent capillary formation. Low-magnification electron micrographs of nascent capillaries formed within exogenous tumors are shown. Note that nascent capillary formation is incomplete and disorganized in tumors grown in Cav-1 KO mice. B shows larger nascent capillaries, while C shows examples of smaller nascent capillaries. Endo, endothelial cell; RBC, red blood cell; Nuc, nucleus. Scale bars: 400 nm ( A ); 2 μm ( B and C ).

    Article Snippet: Samples were examined under a JEOL 1200EX transmission electron microscope (Albert Einstein College of Medicine, Analytical Imaging Facility).

    Techniques: Mouse Assay, Staining, Transmission Assay, Microscopy

    Synthetic procedure for silica-coated Ag 2 S QDs with emission wavelengths in the 840 to 1200 nm spectral range.

    Journal: ACS Nano

    Article Title: Tunable Ultrasmall Visible-to-Extended Near-Infrared Emitting Silver Sulfide Quantum Dots for Integrin-Targeted Cancer Imaging

    doi: 10.1021/nn5071183

    Figure Lengend Snippet: Synthetic procedure for silica-coated Ag 2 S QDs with emission wavelengths in the 840 to 1200 nm spectral range.

    Article Snippet: Sections of 95 nm were cut with a Leica Ultracut UCT ultramicrotome (Leica Microsystems Inc., Bannockburn, IL), stained with uranyl acetate and lead citrate, and viewed on a JEOL 1200 EX transmission electron microscope (JEOL USA Inc., Peabody, MA) equipped with an AMT 8 megapixel digital camera (Advanced Microscopy Techniques, Woburn, MA).

    Techniques:

    Transmission electron microscopy (TEM) of human lung TLA TEM image of a human lung TLA at 50000x (A–C) and 12000x (D). A) The highly integrated nature of the uninfected TLA with healthy cell layers from the microcarrier to the external cell surface. Cell nuclei (N) are indicated. B) Infected lung TL with RSV nucleocapsids in the peri-nuclear region of the cells (VNC). C–D) Budding RSV virions (BV) at the surface of lung TLA. Images were taken using a JEOL-JEM 1010 transmission electron microscope.

    Journal: Methods (San Diego, Calif.)

    Article Title: 3D Tissue-Like Assemblies: A Novel Approach to Investigate Virus-Cell Interactions

    doi: 10.1016/j.ymeth.2015.05.010

    Figure Lengend Snippet: Transmission electron microscopy (TEM) of human lung TLA TEM image of a human lung TLA at 50000x (A–C) and 12000x (D). A) The highly integrated nature of the uninfected TLA with healthy cell layers from the microcarrier to the external cell surface. Cell nuclei (N) are indicated. B) Infected lung TL with RSV nucleocapsids in the peri-nuclear region of the cells (VNC). C–D) Budding RSV virions (BV) at the surface of lung TLA. Images were taken using a JEOL-JEM 1010 transmission electron microscope.

    Article Snippet: Samples were sectioned at silver-gray 6 (100 – 300 μm), mounted on Ni grids, and examined using a JEOL-JEM 1010 transmission electron microscope (JEOL, USA) at 80 kV .

    Techniques: Transmission Assay, Electron Microscopy, Transmission Electron Microscopy, Infection, Microscopy

    P38 inhibitor abolished GFP-PMCA4b internalization in A375 cells, and chloroquine (CQ) trapped the pump in LAMP1 positive intracellular organelles. ( A ) Confocal microscopy images of A375-GFP-PMCA4b cells after treatment with 10 µM p38i-2 inhibitor (SB202190) for 48 h with or without starvation (3 h). The cells were immunostained for the early endosomal marker EEA1. EEA1 and GFP-PMCA4b positive (yellow) vesicles were counted (six cells/group) and expressed as % of total number of GFP-PMCA4b positive vesicles. Scale bar, 20 µm. Bars represent means ± SE. ( B ) Confocal images of A375-GFP-PMCA4b immunostained with the endo-lysosomal markers Rab7, Rab11 and LAMP1, and the autophagy marker LC3 after treatment with CQ for 3 h. The number of GFP-PMCA4b vesicles that co-localize with the endo-lysosomal or autophagy markers were counted and expressed as a % of total number of PMCA4b positive vesicles. Seven to nine cells were counted in each group. Scale bar, 20 µm. Bars represent means ± SE. ( A , B ) Insets show higher magnification view, Scale bar, 10 µM. Arrowheads show co-localization of GFP-PMCA4b with endosomal markers. ( C ) A375-GFP-PMCA4b cells were cultured and treated with CQ for 3 h. Cells were fixed and immunostained with anti-GFP antibody (1:100) (Invitrogen, 1972783). Images were taken by JEM-1011 transmission electron microscope (Jeol). Arrowheads show positive PMCA4b protein ( D ) A375 cells were treated with the proteasome inhibitor MG132 for 6 h at the concentrations indicated. Protein expression was analyzed by Western blotting. The experiment was repeated three times. ( E ) Schematic diagram shows the pathway of PMCA4b degradation through the endo-lysosomal system.

    Journal: Cells

    Article Title: P38 MAPK Promotes Migration and Metastatic Activity of BRAF Mutant Melanoma Cells by Inducing Degradation of PMCA4b

    doi: 10.3390/cells9051209

    Figure Lengend Snippet: P38 inhibitor abolished GFP-PMCA4b internalization in A375 cells, and chloroquine (CQ) trapped the pump in LAMP1 positive intracellular organelles. ( A ) Confocal microscopy images of A375-GFP-PMCA4b cells after treatment with 10 µM p38i-2 inhibitor (SB202190) for 48 h with or without starvation (3 h). The cells were immunostained for the early endosomal marker EEA1. EEA1 and GFP-PMCA4b positive (yellow) vesicles were counted (six cells/group) and expressed as % of total number of GFP-PMCA4b positive vesicles. Scale bar, 20 µm. Bars represent means ± SE. ( B ) Confocal images of A375-GFP-PMCA4b immunostained with the endo-lysosomal markers Rab7, Rab11 and LAMP1, and the autophagy marker LC3 after treatment with CQ for 3 h. The number of GFP-PMCA4b vesicles that co-localize with the endo-lysosomal or autophagy markers were counted and expressed as a % of total number of PMCA4b positive vesicles. Seven to nine cells were counted in each group. Scale bar, 20 µm. Bars represent means ± SE. ( A , B ) Insets show higher magnification view, Scale bar, 10 µM. Arrowheads show co-localization of GFP-PMCA4b with endosomal markers. ( C ) A375-GFP-PMCA4b cells were cultured and treated with CQ for 3 h. Cells were fixed and immunostained with anti-GFP antibody (1:100) (Invitrogen, 1972783). Images were taken by JEM-1011 transmission electron microscope (Jeol). Arrowheads show positive PMCA4b protein ( D ) A375 cells were treated with the proteasome inhibitor MG132 for 6 h at the concentrations indicated. Protein expression was analyzed by Western blotting. The experiment was repeated three times. ( E ) Schematic diagram shows the pathway of PMCA4b degradation through the endo-lysosomal system.

    Article Snippet: Images were taken by JEM-1011 transmission electron microscope (Jeol) equipped with a Morada digital camera (Olympus) using iTEM software (Olympus).

    Techniques: Confocal Microscopy, Marker, Cell Culture, Transmission Assay, Microscopy, Expressing, Western Blot

    Electron microscopy images of chloroplasts from wild type (WT) and the RNAi-W1-7 plants (W1-7) . Leaf segments (2 × 2 mm) from 10 days old primary foliage leaves were taken at a position of 2 cm below the leaf tip. Some of the DNA containing regions are indicated by arrows. Transmission electron microscopy was performed with a Philips CM10 transmission electron microscope. Bars represent 500 nm.

    Journal: Frontiers in Plant Science

    Article Title: WHIRLY1 is a major organizer of chloroplast nucleoids

    doi: 10.3389/fpls.2014.00432

    Figure Lengend Snippet: Electron microscopy images of chloroplasts from wild type (WT) and the RNAi-W1-7 plants (W1-7) . Leaf segments (2 × 2 mm) from 10 days old primary foliage leaves were taken at a position of 2 cm below the leaf tip. Some of the DNA containing regions are indicated by arrows. Transmission electron microscopy was performed with a Philips CM10 transmission electron microscope. Bars represent 500 nm.

    Article Snippet: The sections were stained with saturated uranyl acetate in water and lead citrate (Reynolds, ) and observed using a Philips CM10 transmission electron microscope (FEI, Eindhoven, The Netherlands).

    Techniques: Electron Microscopy, Transmission Assay, Microscopy

    Physicochemical characterization of VCR-loaded T7-LDL. (A) Particle size distribution of VCR-loaded T7-LDL. (B) Morphological appearance of VCR-loaded T7-LDL based on TEM. Stability of VCR-loaded GKRK-APO in the full rat serum. (C) The transmission and backscattering profiles were measured at each time point using a Turbiscan Lab ® Expert analyzer. (D) In vitro release of VCR from T7-LDL and LDL at pH 7.4 at 37 °C, respectively. The data are presented as the means ± SD ( n = 3). *Indicates p

    Journal: Drug Delivery

    Article Title: Enhanced blood–brain barrier penetration and glioma therapy mediated by T7 peptide-modified low-density lipoprotein particles

    doi: 10.1080/10717544.2018.1494223

    Figure Lengend Snippet: Physicochemical characterization of VCR-loaded T7-LDL. (A) Particle size distribution of VCR-loaded T7-LDL. (B) Morphological appearance of VCR-loaded T7-LDL based on TEM. Stability of VCR-loaded GKRK-APO in the full rat serum. (C) The transmission and backscattering profiles were measured at each time point using a Turbiscan Lab ® Expert analyzer. (D) In vitro release of VCR from T7-LDL and LDL at pH 7.4 at 37 °C, respectively. The data are presented as the means ± SD ( n = 3). *Indicates p

    Article Snippet: Morphology of the VCR-loaded T7-LDL was characterized via a transmission electron microscopy (TEM) (HITACHI, H-7650, Japan), respectively.

    Techniques: Transmission Electron Microscopy, Transmission Assay, In Vitro

    PrD-polyQ fusion proteins form seeding-competent amyloid structures in vitro . (A) Schematic representation of MBP fusion proteins with polyQ tracts of different lengths. (B) Time-resolved analysis of polyQ-mediated aggregation of PrD fusions by FRA. SDS-resistant protein aggregates retained on filter membranes were detected using an anti-Sup35 antibody. (C) Electron micrographs of polyQ-containing PrD protein aggregates. MBP fusions were digested with factor Xa without agitation at 37°C for 24h, stained with 1% uranyl acetate and visualised using a Philips CM100 transmission electron microscope. (D) Schematic description of an in vitro seeding assay. The formation of seeding-competent polyQ-containing PrD protein aggregates after cleavage of MBP fusion proteins with factor Xa and the subsequent conversion of HisSup35 protein from the soluble to the amyloidogenic state is shown. SDS-stable protein aggregates were monitored by FRA using specific antibodies. (E) Analysis of HisSup35 aggregate formation by FRA. MBP-PrD-polyQ fusion proteins together with MBP-HisSup35 or MBP-HisCT were incubated with factor Xa at 37°C for 24 and 48 h. Aggregates retained on filter membranes were detected using anti-His and anti-Sup35 antibodies.

    Journal: PLoS ONE

    Article Title: Pathogenic Polyglutamine Tracts Are Potent Inducers of Spontaneous Sup35 and Rnq1 Amyloidogenesis

    doi: 10.1371/journal.pone.0009642

    Figure Lengend Snippet: PrD-polyQ fusion proteins form seeding-competent amyloid structures in vitro . (A) Schematic representation of MBP fusion proteins with polyQ tracts of different lengths. (B) Time-resolved analysis of polyQ-mediated aggregation of PrD fusions by FRA. SDS-resistant protein aggregates retained on filter membranes were detected using an anti-Sup35 antibody. (C) Electron micrographs of polyQ-containing PrD protein aggregates. MBP fusions were digested with factor Xa without agitation at 37°C for 24h, stained with 1% uranyl acetate and visualised using a Philips CM100 transmission electron microscope. (D) Schematic description of an in vitro seeding assay. The formation of seeding-competent polyQ-containing PrD protein aggregates after cleavage of MBP fusion proteins with factor Xa and the subsequent conversion of HisSup35 protein from the soluble to the amyloidogenic state is shown. SDS-stable protein aggregates were monitored by FRA using specific antibodies. (E) Analysis of HisSup35 aggregate formation by FRA. MBP-PrD-polyQ fusion proteins together with MBP-HisSup35 or MBP-HisCT were incubated with factor Xa at 37°C for 24 and 48 h. Aggregates retained on filter membranes were detected using anti-His and anti-Sup35 antibodies.

    Article Snippet: Preparations were viewed using a Philips CM100 transmission electron microscope.

    Techniques: In Vitro, Staining, Transmission Assay, Microscopy, Incubation

    Epithelial- and mesenchymal-type MCAs differ in cross-sectional ultrastructure. Representative epithelial (A-B, OvCa433) and mesenchymal (C-D, Dov13) MCAs were generated via the hanging drop method (Supplemental Figure 1A), observed under light microscope at 4× magnification (A and C), and further processed for TEM as detailed in Methods. Sections were examined under JEOL 1400 TEM (B and D). Scale bar: 10 μm.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Heterogeneous Cadherin Expression and Multicellular Aggregate Dynamics in Ovarian Cancer Dissemination

    doi: 10.1016/j.neo.2017.04.002

    Figure Lengend Snippet: Epithelial- and mesenchymal-type MCAs differ in cross-sectional ultrastructure. Representative epithelial (A-B, OvCa433) and mesenchymal (C-D, Dov13) MCAs were generated via the hanging drop method (Supplemental Figure 1A), observed under light microscope at 4× magnification (A and C), and further processed for TEM as detailed in Methods. Sections were examined under JEOL 1400 TEM (B and D). Scale bar: 10 μm.

    Article Snippet: Ultrathin sections were cut using a Reichert Ultracut microtome, mounted on 100-mesh nickel grids, stained with 5% uranyl acetate and lead Sato's triple lead stain, and examined under JEOL 1400 transmission electron microscope.

    Techniques: Generated, Light Microscopy, Transmission Electron Microscopy

    (A) HRTEM image (inset: TEM image of low magnification); (B) SAED pattern; (C) FTT images; (D) Line-scan EDS; and (E) EDS analysis of Pb 43 Te 56 hollow nanofibers. The electrolyte consisted of 50 mM Pb 2+ , 0.1 mM HTeO 2 + , and 0.1 M HNO 3 at room temperature.

    Journal: Frontiers in Chemistry

    Article Title: Synthesis and Thermoelectric Characterization of Lead Telluride Hollow Nanofibers

    doi: 10.3389/fchem.2018.00436

    Figure Lengend Snippet: (A) HRTEM image (inset: TEM image of low magnification); (B) SAED pattern; (C) FTT images; (D) Line-scan EDS; and (E) EDS analysis of Pb 43 Te 56 hollow nanofibers. The electrolyte consisted of 50 mM Pb 2+ , 0.1 mM HTeO 2 + , and 0.1 M HNO 3 at room temperature.

    Article Snippet: Transmission electron microscopy (TEM), selected area electron diffraction (SAED), field emission-scanning electron microscopy (FE-SEM, FEG-Philips XL30), energy-dispersive X-ray spectroscopy (EDS), and X-ray diffraction (XRD, D8 Advance Diffractometer, Bruker) were used to characterize morphologies, compositions, crystal structures and crystallinity of the nanofiber mats.

    Techniques: Transmission Electron Microscopy

    Transmission electron microscope (TEM) images of density gradient purified AHSV-1 VLPs ( a ), AHSV-1 CLPs ( b ), chimeric AHSV-1/AHSV-7 VLPs ( c ), double chimeric AHSV-1/AHSV-7 VLPs ( d ) and triple chimeric AHSV-1/AHSV-6/AHSV-3 VLPs ( e - f ). Particles were visualized with a JEM-2100 Transmission electron microscope (JEOL). Indicated with red arrows are the virus-like particles (VLPs) and indicated with the blue arrow is a core-like particle (CLP)

    Journal: BMC Veterinary Research

    Article Title: Plant-produced chimeric virus-like particles - a new generation vaccine against African horse sickness

    doi: 10.1186/s12917-019-2184-2

    Figure Lengend Snippet: Transmission electron microscope (TEM) images of density gradient purified AHSV-1 VLPs ( a ), AHSV-1 CLPs ( b ), chimeric AHSV-1/AHSV-7 VLPs ( c ), double chimeric AHSV-1/AHSV-7 VLPs ( d ) and triple chimeric AHSV-1/AHSV-6/AHSV-3 VLPs ( e - f ). Particles were visualized with a JEM-2100 Transmission electron microscope (JEOL). Indicated with red arrows are the virus-like particles (VLPs) and indicated with the blue arrow is a core-like particle (CLP)

    Article Snippet: Transmission electron microscopy (TEM) VLPs from the 55–45% sucrose fractions were visualised by adsorbing samples onto carbon-coated holey copper grids (5 min) and subsequently stained with 2% sodium phosphotungstate, pH = 7 for 30 s. The grids were air dried and subsequently imaged in a JEM-2100 Transmission electron microscope (JEOL).

    Techniques: Transmission Assay, Microscopy, Transmission Electron Microscopy, Purification