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    Millipore promastigote transformation
    Schematic representation of representative mating experiments. Qualitative assessment of recovery of double resistant mutants in mating experiments using M. <t>genitalium</t> strains carrying different antibiotic gene markers. (A) Mating experiments using donor strains (depicted as white mycoplasma cells) carrying the chloramphenicol (Cm R ) and tetracycline (Tet R ) resistance markers and recipient strains (depicted as light gray mycoplasma cells) carrying the puromycin (Pm R ) resistance marker. Donor strains overexpressing σ 20 are indicated as ‘Upσ 20 .’ One of the donor strains used, carried a deletion of the recA gene ( recA - ). Unsuccessful DNA transfers are indicated as empty, dash-lined mycoplasma cells. (B) Mating experiments using a previous transconjugant mutant carrying the chloramphenicol and puromycin resistance markers as a donor strain (depicted as a light gray mycoplasma cell) and a recipient strain (depicted as a dark gray mycoplasma cell) carrying the tetracycline resistance marker. Selection with tetracycline and chloramphenicol results in the isolation of a new transconjugant strain bearing the corresponding antibiotic markers and overexpressing σ 20 . Selection with puromycin and tetracycline allows the isolation of another transconjugant strain with the corresponding antibiotic markers but without the ectopic copy of σ 20 . All the transconjugant strains were genotyped by PCR and sequencing.
    Promastigote Transformation, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/promastigote transformation/product/Millipore
    Average 97 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    promastigote transformation - by Bioz Stars, 2020-05
    97/100 stars
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    Schematic representation of representative mating experiments. Qualitative assessment of recovery of double resistant mutants in mating experiments using M. genitalium strains carrying different antibiotic gene markers. (A) Mating experiments using donor strains (depicted as white mycoplasma cells) carrying the chloramphenicol (Cm R ) and tetracycline (Tet R ) resistance markers and recipient strains (depicted as light gray mycoplasma cells) carrying the puromycin (Pm R ) resistance marker. Donor strains overexpressing σ 20 are indicated as ‘Upσ 20 .’ One of the donor strains used, carried a deletion of the recA gene ( recA - ). Unsuccessful DNA transfers are indicated as empty, dash-lined mycoplasma cells. (B) Mating experiments using a previous transconjugant mutant carrying the chloramphenicol and puromycin resistance markers as a donor strain (depicted as a light gray mycoplasma cell) and a recipient strain (depicted as a dark gray mycoplasma cell) carrying the tetracycline resistance marker. Selection with tetracycline and chloramphenicol results in the isolation of a new transconjugant strain bearing the corresponding antibiotic markers and overexpressing σ 20 . Selection with puromycin and tetracycline allows the isolation of another transconjugant strain with the corresponding antibiotic markers but without the ectopic copy of σ 20 . All the transconjugant strains were genotyped by PCR and sequencing.

    Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

    Article Title: Activation of σ20-dependent recombination and horizontal gene transfer in Mycoplasma genitalium

    doi: 10.1093/dnares/dsy011

    Figure Lengend Snippet: Schematic representation of representative mating experiments. Qualitative assessment of recovery of double resistant mutants in mating experiments using M. genitalium strains carrying different antibiotic gene markers. (A) Mating experiments using donor strains (depicted as white mycoplasma cells) carrying the chloramphenicol (Cm R ) and tetracycline (Tet R ) resistance markers and recipient strains (depicted as light gray mycoplasma cells) carrying the puromycin (Pm R ) resistance marker. Donor strains overexpressing σ 20 are indicated as ‘Upσ 20 .’ One of the donor strains used, carried a deletion of the recA gene ( recA - ). Unsuccessful DNA transfers are indicated as empty, dash-lined mycoplasma cells. (B) Mating experiments using a previous transconjugant mutant carrying the chloramphenicol and puromycin resistance markers as a donor strain (depicted as a light gray mycoplasma cell) and a recipient strain (depicted as a dark gray mycoplasma cell) carrying the tetracycline resistance marker. Selection with tetracycline and chloramphenicol results in the isolation of a new transconjugant strain bearing the corresponding antibiotic markers and overexpressing σ 20 . Selection with puromycin and tetracycline allows the isolation of another transconjugant strain with the corresponding antibiotic markers but without the ectopic copy of σ 20 . All the transconjugant strains were genotyped by PCR and sequencing.

    Article Snippet: Plasmids for M. genitalium transformation were obtained using the GenElute HP Midiprep kit (Sigma).

    Techniques: Marker, Mutagenesis, Selection, Isolation, Polymerase Chain Reaction, Sequencing

    Recombination capacity of different mutants. Graphic showing the recombination capacity of different M. genitalium mutants. Bars represent the averages and the standard deviations of three independent biological repeats. The recombination capacity of the Δ rrlA , Δ rrlB , Δ recA and Δ recA Tnσ 20 mutants was below the limit of detection. Asterisks mark differences that are statistically significant compared with the WT. Statistical significance was assessed using a standard Student’s t test ( p

    Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

    Article Title: Activation of σ20-dependent recombination and horizontal gene transfer in Mycoplasma genitalium

    doi: 10.1093/dnares/dsy011

    Figure Lengend Snippet: Recombination capacity of different mutants. Graphic showing the recombination capacity of different M. genitalium mutants. Bars represent the averages and the standard deviations of three independent biological repeats. The recombination capacity of the Δ rrlA , Δ rrlB , Δ recA and Δ recA Tnσ 20 mutants was below the limit of detection. Asterisks mark differences that are statistically significant compared with the WT. Statistical significance was assessed using a standard Student’s t test ( p

    Article Snippet: Plasmids for M. genitalium transformation were obtained using the GenElute HP Midiprep kit (Sigma).

    Techniques: