transcriptome library Search Results


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  • 90
    Thermo Fisher transcriptome libraries
    Results of BUSCO analysis of the holm oak <t>transcriptome.</t> All the transcriptomes are organized depending on their completeness: RAY-hybrid assembly, RAY-partial hybrid assembly, MIRA-Illumina assembly, RAY-Illumina assembly, MIRA-Ion Torrent assembly; RAY-Ion Torrent assembly; TRINITY-Ion Torrent assembly; and TRINITY-Illumina assembly. Blue: complete and single-copy genes; orange: complete and duplicated genes; grey: fragmented genes; yellow: missing genes.
    Transcriptome Libraries, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcriptome libraries/product/Thermo Fisher
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    Price from $9.99 to $1999.99
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    90
    Thermo Fisher transcriptome library kit
    Results of BUSCO analysis of the holm oak <t>transcriptome.</t> All the transcriptomes are organized depending on their completeness: RAY-hybrid assembly, RAY-partial hybrid assembly, MIRA-Illumina assembly, RAY-Illumina assembly, MIRA-Ion Torrent assembly; RAY-Ion Torrent assembly; TRINITY-Ion Torrent assembly; and TRINITY-Illumina assembly. Blue: complete and single-copy genes; orange: complete and duplicated genes; grey: fragmented genes; yellow: missing genes.
    Transcriptome Library Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcriptome library kit/product/Thermo Fisher
    Average 90 stars, based on 9 article reviews
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    94
    WaferGen Biosystems polya transcriptome library
    Results of BUSCO analysis of the holm oak <t>transcriptome.</t> All the transcriptomes are organized depending on their completeness: RAY-hybrid assembly, RAY-partial hybrid assembly, MIRA-Illumina assembly, RAY-Illumina assembly, MIRA-Ion Torrent assembly; RAY-Ion Torrent assembly; TRINITY-Ion Torrent assembly; and TRINITY-Illumina assembly. Blue: complete and single-copy genes; orange: complete and duplicated genes; grey: fragmented genes; yellow: missing genes.
    Polya Transcriptome Library, supplied by WaferGen Biosystems, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polya transcriptome library/product/WaferGen Biosystems
    Average 94 stars, based on 3 article reviews
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    94
    Illumina Inc transcriptome libraries
    Line graphs (soybean and tomato) and volcano plots (Arabidopsis) showing the change in gene expression for selected groupings of genes and some individual genes . The percent of abscission is indicated in the graph at the top left corner the sections for (A) soybean and (B) tomato as a reference. (A) The Y-axes indicate the percent expression relative to total RPKM for all 37,572 expressed soybean genes <t>(transcriptome).</t> The X-axes indicate the length of exposure to ethylene. Solid circles represent expression in the LAZ and the open triangles in petioles (NAZ-pet). (B) Similar to (A) but The Y-axes indicate the percent expression relative to total microarray signal for 26,527 expressed tomato genes. The X-axes indicate the hours after removal of the flowers, auxin source. Solid circles represent expression in the FAZ and the open triangles in NAZ. The number within the parenthesis to the right of each category or gene name is the number of genes included for that particular graph. Plots with an L2 > 3 after the number in parentheses show the cumulative expression for only those genes with a significant 8-fold increase in expression ( p
    Transcriptome Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 1241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcriptome libraries/product/Illumina Inc
    Average 94 stars, based on 1241 article reviews
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    85
    Stratagene human transcriptome library
    Line graphs (soybean and tomato) and volcano plots (Arabidopsis) showing the change in gene expression for selected groupings of genes and some individual genes . The percent of abscission is indicated in the graph at the top left corner the sections for (A) soybean and (B) tomato as a reference. (A) The Y-axes indicate the percent expression relative to total RPKM for all 37,572 expressed soybean genes <t>(transcriptome).</t> The X-axes indicate the length of exposure to ethylene. Solid circles represent expression in the LAZ and the open triangles in petioles (NAZ-pet). (B) Similar to (A) but The Y-axes indicate the percent expression relative to total microarray signal for 26,527 expressed tomato genes. The X-axes indicate the hours after removal of the flowers, auxin source. Solid circles represent expression in the FAZ and the open triangles in NAZ. The number within the parenthesis to the right of each category or gene name is the number of genes included for that particular graph. Plots with an L2 > 3 after the number in parentheses show the cumulative expression for only those genes with a significant 8-fold increase in expression ( p
    Human Transcriptome Library, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Unigene transcriptome library
    Gene Ontology (GO) analysis of <t>transcriptome</t> associated with flower development of S. arvensis . (The percentage and distribution of top-level GO-terms were portrayed in the three categories: Biological process, Cellular component and Molecular function.). Gene Ontology classifications of assembled unigenes, and 76,324 unigenes with significant similarity in Nr protein databases were assigned to gene ontology classifications.
    Transcriptome Library, supplied by Unigene, used in various techniques. Bioz Stars score: 92/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    BGI Shenzhen transcriptome libraries
    Outer ear morphology-associated genes. Only those genes whose homologous sequences were identified in the donkey white blood cell <t>transcriptome</t> are shown. Pink, mammalian phenotype term; green and red, mouse protein symbols associated with outer ear morphology. Red symbols represent the homologous donkey proteins that are not conserved between donkeys and horses.
    Transcriptome Libraries, supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcriptome libraries/product/BGI Shenzhen
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    91
    Annoroad Gene Technology Co Ltd transcriptome libraries
    Outer ear morphology-associated genes. Only those genes whose homologous sequences were identified in the donkey white blood cell <t>transcriptome</t> are shown. Pink, mammalian phenotype term; green and red, mouse protein symbols associated with outer ear morphology. Red symbols represent the homologous donkey proteins that are not conserved between donkeys and horses.
    Transcriptome Libraries, supplied by Annoroad Gene Technology Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies megaman human transcriptome library
    Outer ear morphology-associated genes. Only those genes whose homologous sequences were identified in the donkey white blood cell <t>transcriptome</t> are shown. Pink, mammalian phenotype term; green and red, mouse protein symbols associated with outer ear morphology. Red symbols represent the homologous donkey proteins that are not conserved between donkeys and horses.
    Megaman Human Transcriptome Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/megaman human transcriptome library/product/Agilent technologies
    Average 88 stars, based on 90 article reviews
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    88
    Stratagene megaman human transcriptome library
    Outer ear morphology-associated genes. Only those genes whose homologous sequences were identified in the donkey white blood cell <t>transcriptome</t> are shown. Pink, mammalian phenotype term; green and red, mouse protein symbols associated with outer ear morphology. Red symbols represent the homologous donkey proteins that are not conserved between donkeys and horses.
    Megaman Human Transcriptome Library, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/megaman human transcriptome library/product/Stratagene
    Average 88 stars, based on 75 article reviews
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    93
    Novogene transcriptome library
    Outer ear morphology-associated genes. Only those genes whose homologous sequences were identified in the donkey white blood cell <t>transcriptome</t> are shown. Pink, mammalian phenotype term; green and red, mouse protein symbols associated with outer ear morphology. Red symbols represent the homologous donkey proteins that are not conserved between donkeys and horses.
    Transcriptome Library, supplied by Novogene, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcriptome library/product/Novogene
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    92
    Agilent technologies transcriptome libraries
    Transcriptomics analysis of cRyr2 Δ50 hearts. A , biological Gene Ontology (PANTHER) groups of significantly changed genes in the c Ryr2 Δ50 heart <t>transcriptome.</t> B–G , changes in the mRNA levels of Ryr2 ( B ), as well as other genes involved in excitation-contraction ( C ), contractility ( D ), energetics ( E ), biosynthetic pathways ( F ), and extracellular matrix ( G ) ( n = 4, *, p ≤ 0.05). All data were plotted as mean ± S.E. Control = Ryr2 flox/wildtype + tamoxifen; c Ryr2 Δ50: Ryr2 flox/wildtype × Mhy6-MerCreMer + + tamoxifen.
    Transcriptome Libraries, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcriptome libraries/product/Agilent technologies
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    91
    Mine Safety Appliances transcriptome library
    Transcriptomics analysis of cRyr2 Δ50 hearts. A , biological Gene Ontology (PANTHER) groups of significantly changed genes in the c Ryr2 Δ50 heart <t>transcriptome.</t> B–G , changes in the mRNA levels of Ryr2 ( B ), as well as other genes involved in excitation-contraction ( C ), contractility ( D ), energetics ( E ), biosynthetic pathways ( F ), and extracellular matrix ( G ) ( n = 4, *, p ≤ 0.05). All data were plotted as mean ± S.E. Control = Ryr2 flox/wildtype + tamoxifen; c Ryr2 Δ50: Ryr2 flox/wildtype × Mhy6-MerCreMer + + tamoxifen.
    Transcriptome Library, supplied by Mine Safety Appliances, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher solid whole transcriptome library kit
    Transcriptomics analysis of cRyr2 Δ50 hearts. A , biological Gene Ontology (PANTHER) groups of significantly changed genes in the c Ryr2 Δ50 heart <t>transcriptome.</t> B–G , changes in the mRNA levels of Ryr2 ( B ), as well as other genes involved in excitation-contraction ( C ), contractility ( D ), energetics ( E ), biosynthetic pathways ( F ), and extracellular matrix ( G ) ( n = 4, *, p ≤ 0.05). All data were plotted as mean ± S.E. Control = Ryr2 flox/wildtype + tamoxifen; c Ryr2 Δ50: Ryr2 flox/wildtype × Mhy6-MerCreMer + + tamoxifen.
    Solid Whole Transcriptome Library Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solid whole transcriptome library kit/product/Thermo Fisher
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    90
    Agilent technologies megamam human transcriptome library
    Transcriptomics analysis of cRyr2 Δ50 hearts. A , biological Gene Ontology (PANTHER) groups of significantly changed genes in the c Ryr2 Δ50 heart <t>transcriptome.</t> B–G , changes in the mRNA levels of Ryr2 ( B ), as well as other genes involved in excitation-contraction ( C ), contractility ( D ), energetics ( E ), biosynthetic pathways ( F ), and extracellular matrix ( G ) ( n = 4, *, p ≤ 0.05). All data were plotted as mean ± S.E. Control = Ryr2 flox/wildtype + tamoxifen; c Ryr2 Δ50: Ryr2 flox/wildtype × Mhy6-MerCreMer + + tamoxifen.
    Megamam Human Transcriptome Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/megamam human transcriptome library/product/Agilent technologies
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    93
    Roche flx transcriptome libraries
    Transcriptomics analysis of cRyr2 Δ50 hearts. A , biological Gene Ontology (PANTHER) groups of significantly changed genes in the c Ryr2 Δ50 heart <t>transcriptome.</t> B–G , changes in the mRNA levels of Ryr2 ( B ), as well as other genes involved in excitation-contraction ( C ), contractility ( D ), energetics ( E ), biosynthetic pathways ( F ), and extracellular matrix ( G ) ( n = 4, *, p ≤ 0.05). All data were plotted as mean ± S.E. Control = Ryr2 flox/wildtype + tamoxifen; c Ryr2 Δ50: Ryr2 flox/wildtype × Mhy6-MerCreMer + + tamoxifen.
    Flx Transcriptome Libraries, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Results of BUSCO analysis of the holm oak transcriptome. All the transcriptomes are organized depending on their completeness: RAY-hybrid assembly, RAY-partial hybrid assembly, MIRA-Illumina assembly, RAY-Illumina assembly, MIRA-Ion Torrent assembly; RAY-Ion Torrent assembly; TRINITY-Ion Torrent assembly; and TRINITY-Illumina assembly. Blue: complete and single-copy genes; orange: complete and duplicated genes; grey: fragmented genes; yellow: missing genes.

    Journal: PLoS ONE

    Article Title: Ion Torrent and lllumina, two complementary RNA-seq platforms for constructing the holm oak (Quercus ilex) transcriptome

    doi: 10.1371/journal.pone.0210356

    Figure Lengend Snippet: Results of BUSCO analysis of the holm oak transcriptome. All the transcriptomes are organized depending on their completeness: RAY-hybrid assembly, RAY-partial hybrid assembly, MIRA-Illumina assembly, RAY-Illumina assembly, MIRA-Ion Torrent assembly; RAY-Ion Torrent assembly; TRINITY-Ion Torrent assembly; and TRINITY-Illumina assembly. Blue: complete and single-copy genes; orange: complete and duplicated genes; grey: fragmented genes; yellow: missing genes.

    Article Snippet: RNA-Seq Library Construction, Ion Torrent sequencing and de novo assembly The cDNA library was built using the Ion Total RNA-Seq Kit v2 for whole transcriptome libraries (Life Technologies Corporation, California, USA), using an aliquot from the same RNA used for Illumina.

    Techniques:

    Experimental work flow showing the steps carried out and bioinformatic utilities used for a transcriptome analysis.

    Journal: PLoS ONE

    Article Title: Ion Torrent and lllumina, two complementary RNA-seq platforms for constructing the holm oak (Quercus ilex) transcriptome

    doi: 10.1371/journal.pone.0210356

    Figure Lengend Snippet: Experimental work flow showing the steps carried out and bioinformatic utilities used for a transcriptome analysis.

    Article Snippet: RNA-Seq Library Construction, Ion Torrent sequencing and de novo assembly The cDNA library was built using the Ion Total RNA-Seq Kit v2 for whole transcriptome libraries (Life Technologies Corporation, California, USA), using an aliquot from the same RNA used for Illumina.

    Techniques: Flow Cytometry

    Alignment between Q . robur and Q . petrea transcriptomes (oak transcriptome) and Q . ilex (holm oak) transcriptome using MIRA, RAY, TRINITY and RAY hybrid assemblies from Illumina ( a ) and Ion Torrent ( b ) reads. Distribution of percent sequence identity between oak and Q . ilex (MIRA, RAY, TRINITY, RAY hybrids) transcriptomes ( c ).

    Journal: PLoS ONE

    Article Title: Ion Torrent and lllumina, two complementary RNA-seq platforms for constructing the holm oak (Quercus ilex) transcriptome

    doi: 10.1371/journal.pone.0210356

    Figure Lengend Snippet: Alignment between Q . robur and Q . petrea transcriptomes (oak transcriptome) and Q . ilex (holm oak) transcriptome using MIRA, RAY, TRINITY and RAY hybrid assemblies from Illumina ( a ) and Ion Torrent ( b ) reads. Distribution of percent sequence identity between oak and Q . ilex (MIRA, RAY, TRINITY, RAY hybrids) transcriptomes ( c ).

    Article Snippet: RNA-Seq Library Construction, Ion Torrent sequencing and de novo assembly The cDNA library was built using the Ion Total RNA-Seq Kit v2 for whole transcriptome libraries (Life Technologies Corporation, California, USA), using an aliquot from the same RNA used for Illumina.

    Techniques: Sequencing

    Bioinformatics workflow for transcriptome and genome analysis. Figshare doi: 10.6084/m9.figshare.4056069 .

    Journal: PeerJ

    Article Title: Genomic and transcriptomic resources for assassin flies including the complete genome sequence of Proctacanthus coquilletti (Insecta: Diptera: Asilidae) and 16 representative transcriptomes

    doi: 10.7717/peerj.2951

    Figure Lengend Snippet: Bioinformatics workflow for transcriptome and genome analysis. Figshare doi: 10.6084/m9.figshare.4056069 .

    Article Snippet: For the specimens sequenced using Ion Torrent, the isolation of mRNA was carried out using DynaBeads mRNA DIRECT Kit, and Ion Total RNA-Seq Kit (v2) for Whole Transcriptome Libraries (Thermo Fisher Scientific) was used for library preparation.

    Techniques:

    Line graphs (soybean and tomato) and volcano plots (Arabidopsis) showing the change in gene expression for selected groupings of genes and some individual genes . The percent of abscission is indicated in the graph at the top left corner the sections for (A) soybean and (B) tomato as a reference. (A) The Y-axes indicate the percent expression relative to total RPKM for all 37,572 expressed soybean genes (transcriptome). The X-axes indicate the length of exposure to ethylene. Solid circles represent expression in the LAZ and the open triangles in petioles (NAZ-pet). (B) Similar to (A) but The Y-axes indicate the percent expression relative to total microarray signal for 26,527 expressed tomato genes. The X-axes indicate the hours after removal of the flowers, auxin source. Solid circles represent expression in the FAZ and the open triangles in NAZ. The number within the parenthesis to the right of each category or gene name is the number of genes included for that particular graph. Plots with an L2 > 3 after the number in parentheses show the cumulative expression for only those genes with a significant 8-fold increase in expression ( p

    Journal: Frontiers in Plant Science

    Article Title: Examination of the Abscission-Associated Transcriptomes for Soybean, Tomato, and Arabidopsis Highlights the Conserved Biosynthesis of an Extensible Extracellular Matrix and Boundary Layer

    doi: 10.3389/fpls.2015.01109

    Figure Lengend Snippet: Line graphs (soybean and tomato) and volcano plots (Arabidopsis) showing the change in gene expression for selected groupings of genes and some individual genes . The percent of abscission is indicated in the graph at the top left corner the sections for (A) soybean and (B) tomato as a reference. (A) The Y-axes indicate the percent expression relative to total RPKM for all 37,572 expressed soybean genes (transcriptome). The X-axes indicate the length of exposure to ethylene. Solid circles represent expression in the LAZ and the open triangles in petioles (NAZ-pet). (B) Similar to (A) but The Y-axes indicate the percent expression relative to total microarray signal for 26,527 expressed tomato genes. The X-axes indicate the hours after removal of the flowers, auxin source. Solid circles represent expression in the FAZ and the open triangles in NAZ. The number within the parenthesis to the right of each category or gene name is the number of genes included for that particular graph. Plots with an L2 > 3 after the number in parentheses show the cumulative expression for only those genes with a significant 8-fold increase in expression ( p

    Article Snippet: “VF-36.” Transcriptome libraries for sequencing were constructed according to the Illumina TruSeq RNA library protocol outlined in “TruSeq RNA Sample Preparation Guide” (Part # 15008136; Rev.

    Techniques: Expressing, Positron Emission Tomography, Microarray

    Positions of single nucleotide polymorphisms (SNPs). A SNP positions in the transcriptomes of MNOP. B SNP position in the transcriptomes of MNON.

    Journal: PLoS ONE

    Article Title: Insights into Sexual Precocity of Female Oriental River Prawn Macrobrachium nipponense through Transcriptome Analysis

    doi: 10.1371/journal.pone.0157173

    Figure Lengend Snippet: Positions of single nucleotide polymorphisms (SNPs). A SNP positions in the transcriptomes of MNOP. B SNP position in the transcriptomes of MNON.

    Article Snippet: Transcriptome library preparation and Illumina sequencing RNA purification, reverse transcription, library construction and sequencing were performed at Shanghai Majorbio Bio-pharm Biotechnology Co., Ltd. (Shanghai, China) according to the manufacturer’s instructions (Illumina, San Diego, CA).

    Techniques:

    Inhibitory Fc receptor gene upregulation in B cells during CHB revealed by RNA sequencing. Transcriptome analysis of CD19+ sorted peripheral blood lymphocytes of CHB (HepB+ or pos) and controls patients (HepB− or neg) (n = 5 each). ( A ) Euclidian distance illustrates hierarchical clustering of the groups. ( B ) Principal component analysis differentiates between HBV+ and HBV− conditions and shows variance amongst replicates. ( C ) Heat map of subset of differentially expressed genes (FDR corrected p

    Journal: Scientific Reports

    Article Title: HBV induces inhibitory FcRL receptor on B cells and dysregulates B cell-T follicular helper cell axis

    doi: 10.1038/s41598-018-33719-x

    Figure Lengend Snippet: Inhibitory Fc receptor gene upregulation in B cells during CHB revealed by RNA sequencing. Transcriptome analysis of CD19+ sorted peripheral blood lymphocytes of CHB (HepB+ or pos) and controls patients (HepB− or neg) (n = 5 each). ( A ) Euclidian distance illustrates hierarchical clustering of the groups. ( B ) Principal component analysis differentiates between HBV+ and HBV− conditions and shows variance amongst replicates. ( C ) Heat map of subset of differentially expressed genes (FDR corrected p

    Article Snippet: Whole transcriptome libraries were constructed for sequencing on the Illumina platform using the NEBNext® Ultra™ Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA).

    Techniques: RNA Sequencing Assay

    Scatter plots showing the correlation of the gene expression profiles between the control and Zn-exposed groups. X- and Y-axes present log 2  values of gene expression. Differentially expressed genes are indicated in orange (up-regulated expression) and blue (down-regulated expression). Brown indicates genes that were not differentially expressed.

    Journal: Scientific Reports

    Article Title: Role and mechanism of the AMPK pathway in waterborne Zn exposure influencing the hepatic energy metabolism of Synechogobius hasta

    doi: 10.1038/srep38716

    Figure Lengend Snippet: Scatter plots showing the correlation of the gene expression profiles between the control and Zn-exposed groups. X- and Y-axes present log 2 values of gene expression. Differentially expressed genes are indicated in orange (up-regulated expression) and blue (down-regulated expression). Brown indicates genes that were not differentially expressed.

    Article Snippet: Library preparation and Illumina sequencing The transcriptome library construction and sequencing were performed at the Beijing Genome Institute (BGI, Shenzhen, China).

    Techniques: Expressing

    Gene Ontology (GO) analysis of transcriptome associated with flower development of S. arvensis . (The percentage and distribution of top-level GO-terms were portrayed in the three categories: Biological process, Cellular component and Molecular function.). Gene Ontology classifications of assembled unigenes, and 76,324 unigenes with significant similarity in Nr protein databases were assigned to gene ontology classifications.

    Journal: PLoS ONE

    Article Title: Deep RNA-Seq to Unlock the Gene Bank of Floral Development in Sinapis arvensis

    doi: 10.1371/journal.pone.0105775

    Figure Lengend Snippet: Gene Ontology (GO) analysis of transcriptome associated with flower development of S. arvensis . (The percentage and distribution of top-level GO-terms were portrayed in the three categories: Biological process, Cellular component and Molecular function.). Gene Ontology classifications of assembled unigenes, and 76,324 unigenes with significant similarity in Nr protein databases were assigned to gene ontology classifications.

    Article Snippet: To evaluate the completeness of our transcriptome library and the effectiveness of our annotation process, we searched the annotated unigene sequences for the possible functions involved in COG classifications.

    Techniques:

    Outer ear morphology-associated genes. Only those genes whose homologous sequences were identified in the donkey white blood cell transcriptome are shown. Pink, mammalian phenotype term; green and red, mouse protein symbols associated with outer ear morphology. Red symbols represent the homologous donkey proteins that are not conserved between donkeys and horses.

    Journal: PLoS ONE

    Article Title: De Novo Assembly of the Donkey White Blood Cell Transcriptome and a Comparative Analysis of Phenotype-Associated Genes between Donkeys and Horses

    doi: 10.1371/journal.pone.0133258

    Figure Lengend Snippet: Outer ear morphology-associated genes. Only those genes whose homologous sequences were identified in the donkey white blood cell transcriptome are shown. Pink, mammalian phenotype term; green and red, mouse protein symbols associated with outer ear morphology. Red symbols represent the homologous donkey proteins that are not conserved between donkeys and horses.

    Article Snippet: The isolated white cells were stored in RNAlater Stabilization Solution (AM7020, Life Technologies) and sent to the Beijing Genome Institute (BGI-Shenzhen) for transcriptome library construction and RNA-seq.

    Techniques:

    Transcriptomics analysis of cRyr2 Δ50 hearts. A , biological Gene Ontology (PANTHER) groups of significantly changed genes in the c Ryr2 Δ50 heart transcriptome. B–G , changes in the mRNA levels of Ryr2 ( B ), as well as other genes involved in excitation-contraction ( C ), contractility ( D ), energetics ( E ), biosynthetic pathways ( F ), and extracellular matrix ( G ) ( n = 4, *, p ≤ 0.05). All data were plotted as mean ± S.E. Control = Ryr2 flox/wildtype + tamoxifen; c Ryr2 Δ50: Ryr2 flox/wildtype × Mhy6-MerCreMer + + tamoxifen.

    Journal: The Journal of Biological Chemistry

    Article Title: Cardiac Ryanodine Receptor (Ryr2)-mediated Calcium Signals Specifically Promote Glucose Oxidation via Pyruvate Dehydrogenase *

    doi: 10.1074/jbc.M116.756973

    Figure Lengend Snippet: Transcriptomics analysis of cRyr2 Δ50 hearts. A , biological Gene Ontology (PANTHER) groups of significantly changed genes in the c Ryr2 Δ50 heart transcriptome. B–G , changes in the mRNA levels of Ryr2 ( B ), as well as other genes involved in excitation-contraction ( C ), contractility ( D ), energetics ( E ), biosynthetic pathways ( F ), and extracellular matrix ( G ) ( n = 4, *, p ≤ 0.05). All data were plotted as mean ± S.E. Control = Ryr2 flox/wildtype + tamoxifen; c Ryr2 Δ50: Ryr2 flox/wildtype × Mhy6-MerCreMer + + tamoxifen.

    Article Snippet: Yield and size distribution of the transcriptome libraries were assessed using the Agilent High Sensitivity DNA Kit on the 2100 Bioanalyzer.

    Techniques: