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Image Search Results

Journal: The Journal of Neuroscience
Article Title: Human Astrocytes Are Resistant to Fas Ligand and Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand-Induced Apoptosis
doi: 10.1523/JNEUROSCI.5572-05.2006
Figure Lengend Snippet: Insufficient expression of TRAIL receptors blocks TRAIL-induced signal pathway in human astrocytes. A, B, Analysis of Fas agonist (A) and TRAIL-induced DISC (B) in human astrocytes and human glioma U343MG cells. The DISC was immunoprecipitated by the treatment of human astrocytes with either Fas agonist antibody or Flag-tagged TRAIL for 15 min in stimulated cells (+) or after cell lysis in unstimulated cells (−). Cell lysates obtained from untreated cells (Lysate) were included as control for the expression of the endogenous proteins. C, TRAIL sensitivity of human astrocytes transfected with DR5 cDNA. Human astrocytes were transfected with DR5 cDNA for 48 h and treated with KN93 (100 μm) and TRAIL (1 μg/ml), alone or in combination as indicated at the top. After 6 h of treatment, the cells were subjected to Western blot for DR5 expression (top) and caspase-3 cleavage (middle). After 24 h of treatment, the cells were analyzed for cell viability (bottom) (mean ± SEM; n = 6; *p < 0.05).
Article Snippet: The following six antibodies against human DR4 and DR5 were used for Western blots: mouse monoclonal ab1-DR4 and ab1-DR5 (Alexis Biochemicals), rabbit antibody ab2-DR4 (Imgenex, San Diego, CA), ab2-DR4 (Chemicon, Temecula, CA),
Techniques: Expressing, Immunoprecipitation, Lysis, Transfection, Western Blot