Journal: Data in Brief

Article Title: Whole transcriptome sequence data of 5-FU sensitive and 5-FU resistant tumors generated in a mouse model of de novo carcinogenesis

doi: 10.1016/j.dib.2018.08.209

Figure Lengend Snippet: Generation of 5-FU sensitive and resistant tumors in a mouse model of chemically-induced carcinogenesis. Carcinogenesis was initiated with DMBA, followed by bi-weekly treatments with TPA to promote tumor growth. Isolated RNA from 2 pairs of 5-FU sensitive and 5-FU resistant tumors was used to perform whole-transcriptome sequencing using Illumina 2000 , in order to identify differentially expressed genes between sensitive and resistant tumors.

Article Snippet: Two weeks after DMBA administration, we initiated treatment with 2.5 μg TPA diluted in 200 μl acetone twice a week (Sigma-Aldrich, cat. #P8139) until a mouse was sacrificed.

Techniques: Isolation, Sequencing

Journal: PLoS ONE

Article Title: Subacute Intranasal Administration of Tissue Plasminogen Activator Promotes Neuroplasticity and Improves Functional Recovery following Traumatic Brain Injury in Rats

doi: 10.1371/journal.pone.0106238

Figure Lengend Snippet: Cortical lesion volume after TBI and tPA treatment. The bar graph shows no significance (NS) in the cortical lesion volume between the TBI+Saline and TBI+tPA groups examined at 35 days post injury ( p > 0.05). Scale bar = 2 mm. Data represent mean ± SD. n = 8 (rats/group).

Article Snippet: Sham+tPA rats (n = 8) underwent surgery without injury but received the same dose of intranasal tPA as did the TBI+tPA rats. tPA at a dose of 600 µg/rat (Genentech, San Francisco, CA) was administered intranasally at Days 7 and 14 post TBI or sham-operation.

Techniques:

Journal: PLoS ONE

Article Title: Subacute Intranasal Administration of Tissue Plasminogen Activator Promotes Neuroplasticity and Improves Functional Recovery following Traumatic Brain Injury in Rats

doi: 10.1371/journal.pone.0106238

Figure Lengend Snippet: Correlation of the total length of axons crossing the midline at the cervical spinal cord with the right forelimb foot fault (A) and the mNSS score (B). The line graph shows that the total length of axonal crossing at the midline of the cervical level of the spinal cord is significantly reversely correlated with the incidence of forelimb footfault (A) and mNSS score (B) in rats examined at 35 days after TBI and tPA treatment ( p

Article Snippet: Sham+tPA rats (n = 8) underwent surgery without injury but received the same dose of intranasal tPA as did the TBI+tPA rats. tPA at a dose of 600 µg/rat (Genentech, San Francisco, CA) was administered intranasally at Days 7 and 14 post TBI or sham-operation.

Techniques:

Journal: PLoS ONE

Article Title: Subacute Intranasal Administration of Tissue Plasminogen Activator Promotes Neuroplasticity and Improves Functional Recovery following Traumatic Brain Injury in Rats

doi: 10.1371/journal.pone.0106238

Figure Lengend Snippet: Western blot analysis of ProBDNF and mature BDNF protein levels in the brain and spinal cord. Left panel (ipsilateral brain cortex): Sham+tPA (1, 2), TBI+Saline (3, 4); 3: TBI+tPA (5, 6); Right panel (denervated cervical spinal cord): Sham+tPA (1, 2), TBI+Saline (3, 4), TBI+tPA (5, 6). * p

Article Snippet: Sham+tPA rats (n = 8) underwent surgery without injury but received the same dose of intranasal tPA as did the TBI+tPA rats. tPA at a dose of 600 µg/rat (Genentech, San Francisco, CA) was administered intranasally at Days 7 and 14 post TBI or sham-operation.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Subacute Intranasal Administration of Tissue Plasminogen Activator Promotes Neuroplasticity and Improves Functional Recovery following Traumatic Brain Injury in Rats

doi: 10.1371/journal.pone.0106238

Figure Lengend Snippet: tPA effect on functional outcome. tPA significantly lowered the mNSS scores (A) and reduced frequency of foot faults (B) from Day 14 to 35 after TBI compared to the saline group. tPA treatment significantly improved spatial learning performance from Day 33 to 35 after TBI compared with the saline group (C). * p

Article Snippet: Sham+tPA rats (n = 8) underwent surgery without injury but received the same dose of intranasal tPA as did the TBI+tPA rats. tPA at a dose of 600 µg/rat (Genentech, San Francisco, CA) was administered intranasally at Days 7 and 14 post TBI or sham-operation.

Techniques: Functional Assay

Journal: PLoS ONE

Article Title: Subacute Intranasal Administration of Tissue Plasminogen Activator Promotes Neuroplasticity and Improves Functional Recovery following Traumatic Brain Injury in Rats

doi: 10.1371/journal.pone.0106238

Figure Lengend Snippet: tPA effect on immature neurons after TBI. DCX staining (A–C). tPA significantly increased immature neurons identified with DCX-positive staining in the DG in rats examined 35 days after injury compared with the saline-treated group. The bar graph shows the number of DCX-positive cells. Scale bar = 25 µm. * p

Article Snippet: Sham+tPA rats (n = 8) underwent surgery without injury but received the same dose of intranasal tPA as did the TBI+tPA rats. tPA at a dose of 600 µg/rat (Genentech, San Francisco, CA) was administered intranasally at Days 7 and 14 post TBI or sham-operation.

Techniques: Staining

Journal: PLoS ONE

Article Title: Subacute Intranasal Administration of Tissue Plasminogen Activator Promotes Neuroplasticity and Improves Functional Recovery following Traumatic Brain Injury in Rats

doi: 10.1371/journal.pone.0106238

Figure Lengend Snippet: tPA protein level and activity as well as plasmin activity in rat brain. Western blot analysis of tPA protein levels in the rat brain 24 hr after intranasal administration of tPA (A). Bar graph (B) shows the tPA protein level. Of note, Sham+tPA rats received the same amount of intranasal tPA administration as TBI+tPA rats did. Representative zymographic assay (C) shows an increase in tPA activity in the Sham+tPA rats and TBI+tPA rats compared to TBI+Saline rats. h-r-tPA: human recombinant tPA (15 ng, Genentech). Bar graph (D) shows the tPA activity. Bar graph (E) shows amidolytic activity of plasmin assayed with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride (S-2251) as its specific substrate. Bar graph (F) shows tPA amidolytic activity with S-2251 as the substrate in the presence of added plasminogen compared to Sham. * p

Article Snippet: Sham+tPA rats (n = 8) underwent surgery without injury but received the same dose of intranasal tPA as did the TBI+tPA rats. tPA at a dose of 600 µg/rat (Genentech, San Francisco, CA) was administered intranasally at Days 7 and 14 post TBI or sham-operation.

Techniques: Activity Assay, Western Blot, Recombinant

Journal: PLoS ONE

Article Title: Subacute Intranasal Administration of Tissue Plasminogen Activator Promotes Neuroplasticity and Improves Functional Recovery following Traumatic Brain Injury in Rats

doi: 10.1371/journal.pone.0106238

Figure Lengend Snippet: BDA-labeling of CST originating from the contralesional intact hemisphere. Representative images from the cervical spinal cord show BDA-labeled CST axons crossing the midline (arrows in B) and sprouting into the denervated side of the ventral gray matter in a rat after TBI. tPA treatment significantly increased the axon midline crossing (arrows in C). There were no obvious BDA-labeled axons observed in the opposite side of the cervical spinal cord in sham rats (A). Quantitative data (D) show that the number of contralesional CST in the denervated cervical gray matter was increased significantly by traumatic injury ( p

Article Snippet: Sham+tPA rats (n = 8) underwent surgery without injury but received the same dose of intranasal tPA as did the TBI+tPA rats. tPA at a dose of 600 µg/rat (Genentech, San Francisco, CA) was administered intranasally at Days 7 and 14 post TBI or sham-operation.

Techniques: Labeling

Journal: PLoS ONE

Article Title: Subacute Intranasal Administration of Tissue Plasminogen Activator Promotes Neuroplasticity and Improves Functional Recovery following Traumatic Brain Injury in Rats

doi: 10.1371/journal.pone.0106238

Figure Lengend Snippet: tPA effect on neurogenesis after TBI. Compared to the TBI+Saline group (B), tPA treatment (C) significantly increased newborn mature neurons identified with BrdU/NeuN double immunofluorescent staining in the DG 35 days post injury. The bar graph (D) shows the number of DCX-positive cells. Scale bar = 25 µm. * p

Article Snippet: Sham+tPA rats (n = 8) underwent surgery without injury but received the same dose of intranasal tPA as did the TBI+tPA rats. tPA at a dose of 600 µg/rat (Genentech, San Francisco, CA) was administered intranasally at Days 7 and 14 post TBI or sham-operation.

Techniques: Staining

Journal: PLoS ONE

Article Title: Subacute Intranasal Administration of Tissue Plasminogen Activator Promotes Neuroplasticity and Improves Functional Recovery following Traumatic Brain Injury in Rats

doi: 10.1371/journal.pone.0106238

Figure Lengend Snippet: Correlation of the number of neuroblasts (A), and newborn neurons (B) with spatial learning. The line graph shows that spatial learning is significantly correlated with the number of DCX-positive cells (A) and to the number of newborn mature neurons (B) in the DG of the ipsilateral hippocampus in rats examined at 35 days after TBI and tPA treatment ( p

Article Snippet: Sham+tPA rats (n = 8) underwent surgery without injury but received the same dose of intranasal tPA as did the TBI+tPA rats. tPA at a dose of 600 µg/rat (Genentech, San Francisco, CA) was administered intranasally at Days 7 and 14 post TBI or sham-operation.

Techniques: