Article Title: A long non-coding RNA protects the heart from pathological hypertrophy
Figure Lengend Snippet: Mhrt inhibits cardiac hypertrophy and failure a, Quantitation of cardiac Mhrt s 2–42 days after TAC operation. P-value: Student’s t-test. Error bar: SEM. b, RT-PCR of Mhrts in adult heart ventricles. Primers (F1 and R1, Fig. 1a ) target Mhrt common regions. Size controls 779, 826, 709 are PCR products of recombinant Mhrt779, Mhrt826 , and Mhrt709 , respectively. c , Northern blot of Mhrts in adult heart ventricles. The probe targets common regions of Mhrts . Negative: control RNA from 293T cells. Size control 826 is recombinant Mhrt826 ; 643 (not a distinct Mhrt species) contains the 5′ common region of Mhrt . d Quantitation of Mhrt779 in control or Tg779 mice with/without doxycycline (Dox) or TAC operation. Mhrt779 -specific PCR primers were used. Ctrl: control mice. Tg779 : Tnnt2-rtTA ; Tre-Mhrt779 mice. P-value: Student’s t-test. Error bar: standard error of the mean (SEM). e, Ventricle-body weight ratio of hearts 6 weeks after TAC. P-value: Student’s t-test. Error bar: SEM. Scale=1 mm. f, Quantitation of cardiomyocyte size in control and Tg779 mice 6 weeks after TAC by wheat-germ agglutinin staining. P-value: Student’s t-test. Error bar: SEM. g, Trichrome staining in control and Tg779 hearts 6 weeks after TAC. Red: cardiomyocytes. Blue: fibrosis. h, i, Echocardiographic measurement of left ventricular fractional shortening ( h ) and internal dimensions at end-diastole (LVIDd) and end-systole (LVIDs) ( i ) 6 weeks after TAC. P-value: Student’s t-test. Error bar: SEM.
Article Snippet: To conduct strand specific RT PCR analysis, human total RNA and Superscript III First-Strand Synthesis System (Invitrogen) was used.
Techniques: Quantitation Assay, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Recombinant, Northern Blot, Negative Control, Mouse Assay, Staining