Journal:

Article Title: Endothelial-Specific Expression of Mitochondrial Thioredoxin Improves Endothelial Cell Function and Reduces Atherosclerotic Lesions

doi: 10.2353/ajpath.2007.060960

Figure Lengend Snippet: Hemodynamics in Trx2 TG mice. Serum from WT and Trx2 TG mice (both lines 1 and 3) were collected. a: Total antioxidant levels in the serum were measured by the Total Anti-Oxidant Status assay kit (Calbiochem) b: 8-Isoprostane, a systemic oxidative marker in serum, was determined by the enzyme-linked immunosorbent assay kit (Calbiochem). c: Serum NO was measured as NOx for both nitrite and nitrate (Cayman). d: Basal heart rate (minutes) was determined by echocardiography. e–g: End diastolic pressure, end systolic pressure, and mean artery pressure were determined at baseline and after administration of NO inhibitor l-NAME (0.1 mg/10 g body weight for 30 minutes). The number of mice in each group is shown in parentheses. *P < 0.05, statistical significance between WT and Trx2 TG groups, n = 9 in each group.

Article Snippet: Serum from WT and Trx2 TG mice (both lines 1 and 3) were collected. a: Total antioxidant levels in the serum were measured by the Total Anti-Oxidant Status assay kit (Calbiochem) b: 8-Isoprostane, a systemic oxidative marker in serum, was determined by the enzyme-linked immunosorbent assay kit (Calbiochem). c: Serum NO was measured as NOx for both nitrite and nitrate (Cayman). d: Basal heart rate (minutes) was determined by echocardiography . e–g: End diastolic pressure, end systolic pressure, and mean artery pressure were determined at baseline and after administration of NO inhibitor l -NAME (0.1 mg/10 g body weight for 30 minutes).

Techniques: Marker, Enzyme-linked Immunosorbent Assay