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  • 90
    Thermo Fisher zero blunt topo pcr cloning kit s
    Zero Blunt Topo Pcr Cloning Kit S, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher xl topo ta cloning kit
    Xl Topo Ta Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher topo xl prc cloning kit
    Topo Xl Prc Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher topo xl pcr cloning kit
    Distinctive P Elements Revealed by Re-screening a Random Sample of P Element Insertion Sites in Natural Populations for Four Genes, Hsp23, Hsp27, Hsrω, and Hsp70 The P element insertion sites were selected from Gene Set I. A plus sign (+) indicates successful <t>PCR</t> amplification with one primer complementary to the focal gene and another complementary to a unique sequence in the P element (top), and thus reports the size and orientation of the P element; a minus sign (−) indicates no amplification. Table S7 provides sequences of these primers. At each insertion site in a population, one to six distinctive P elements segregated; these are designated a–f. For Hsp23, Hsp27, and Hsrω, nine insertion sites shared by two or more natural populations (indicated by boxes) and 17 unique insertion sites were re-screened. <t>Amplicons</t> that share a symbol (filled square [█], filled triangle [▴], filled circle •], etc.) occurred at the same integration site in different populations and were indistinguishable by size or orientation. For Hsp70, a five-copy gene in natural populations [ 17 ], the specific gene of insertion was not determined; thus, each distinctive amplicon (a–f) could represent insertion(s) at the same site in one to five of the Hsp70 genes. For population codes see Figure 1 . ORF, open reading frame.
    Topo Xl Pcr Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1573 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher topo xl pcr cloning kit protocol
    Distinctive P Elements Revealed by Re-screening a Random Sample of P Element Insertion Sites in Natural Populations for Four Genes, Hsp23, Hsp27, Hsrω, and Hsp70 The P element insertion sites were selected from Gene Set I. A plus sign (+) indicates successful <t>PCR</t> amplification with one primer complementary to the focal gene and another complementary to a unique sequence in the P element (top), and thus reports the size and orientation of the P element; a minus sign (−) indicates no amplification. Table S7 provides sequences of these primers. At each insertion site in a population, one to six distinctive P elements segregated; these are designated a–f. For Hsp23, Hsp27, and Hsrω, nine insertion sites shared by two or more natural populations (indicated by boxes) and 17 unique insertion sites were re-screened. <t>Amplicons</t> that share a symbol (filled square [█], filled triangle [▴], filled circle •], etc.) occurred at the same integration site in different populations and were indistinguishable by size or orientation. For Hsp70, a five-copy gene in natural populations [ 17 ], the specific gene of insertion was not determined; thus, each distinctive amplicon (a–f) could represent insertion(s) at the same site in one to five of the Hsp70 genes. For population codes see Figure 1 . ORF, open reading frame.
    Topo Xl Pcr Cloning Kit Protocol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher topo xl 2 complete pcr cloning kit
    Distinctive P Elements Revealed by Re-screening a Random Sample of P Element Insertion Sites in Natural Populations for Four Genes, Hsp23, Hsp27, Hsrω, and Hsp70 The P element insertion sites were selected from Gene Set I. A plus sign (+) indicates successful <t>PCR</t> amplification with one primer complementary to the focal gene and another complementary to a unique sequence in the P element (top), and thus reports the size and orientation of the P element; a minus sign (−) indicates no amplification. Table S7 provides sequences of these primers. At each insertion site in a population, one to six distinctive P elements segregated; these are designated a–f. For Hsp23, Hsp27, and Hsrω, nine insertion sites shared by two or more natural populations (indicated by boxes) and 17 unique insertion sites were re-screened. <t>Amplicons</t> that share a symbol (filled square [█], filled triangle [▴], filled circle •], etc.) occurred at the same integration site in different populations and were indistinguishable by size or orientation. For Hsp70, a five-copy gene in natural populations [ 17 ], the specific gene of insertion was not determined; thus, each distinctive amplicon (a–f) could represent insertion(s) at the same site in one to five of the Hsp70 genes. For population codes see Figure 1 . ORF, open reading frame.
    Topo Xl 2 Complete Pcr Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher xl topo kit
    Distinctive P Elements Revealed by Re-screening a Random Sample of P Element Insertion Sites in Natural Populations for Four Genes, Hsp23, Hsp27, Hsrω, and Hsp70 The P element insertion sites were selected from Gene Set I. A plus sign (+) indicates successful <t>PCR</t> amplification with one primer complementary to the focal gene and another complementary to a unique sequence in the P element (top), and thus reports the size and orientation of the P element; a minus sign (−) indicates no amplification. Table S7 provides sequences of these primers. At each insertion site in a population, one to six distinctive P elements segregated; these are designated a–f. For Hsp23, Hsp27, and Hsrω, nine insertion sites shared by two or more natural populations (indicated by boxes) and 17 unique insertion sites were re-screened. <t>Amplicons</t> that share a symbol (filled square [█], filled triangle [▴], filled circle •], etc.) occurred at the same integration site in different populations and were indistinguishable by size or orientation. For Hsp70, a five-copy gene in natural populations [ 17 ], the specific gene of insertion was not determined; thus, each distinctive amplicon (a–f) could represent insertion(s) at the same site in one to five of the Hsp70 genes. For population codes see Figure 1 . ORF, open reading frame.
    Xl Topo Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcr cloning kit
    Distinctive P Elements Revealed by Re-screening a Random Sample of P Element Insertion Sites in Natural Populations for Four Genes, Hsp23, Hsp27, Hsrω, and Hsp70 The P element insertion sites were selected from Gene Set I. A plus sign (+) indicates successful <t>PCR</t> amplification with one primer complementary to the focal gene and another complementary to a unique sequence in the P element (top), and thus reports the size and orientation of the P element; a minus sign (−) indicates no amplification. Table S7 provides sequences of these primers. At each insertion site in a population, one to six distinctive P elements segregated; these are designated a–f. For Hsp23, Hsp27, and Hsrω, nine insertion sites shared by two or more natural populations (indicated by boxes) and 17 unique insertion sites were re-screened. <t>Amplicons</t> that share a symbol (filled square [█], filled triangle [▴], filled circle •], etc.) occurred at the same integration site in different populations and were indistinguishable by size or orientation. For Hsp70, a five-copy gene in natural populations [ 17 ], the specific gene of insertion was not determined; thus, each distinctive amplicon (a–f) could represent insertion(s) at the same site in one to five of the Hsp70 genes. For population codes see Figure 1 . ORF, open reading frame.
    Pcr Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher topo xl 2 kit
    Multiple-sequence alignment of amplicons and cDNA clones. Multiple-sequence alignment of cDNA amplicons and cDNA clones ( a and c ) and Kos_KSP amplicons and clones ( b and d ). a In-Fusion cloning of cDNA amplicons generated from the serum of an infected pig. The cDNA amplicons used for the cloning is shown as the first sequence. b In-Fusion cloning of Kos_KSP derived amplicons. c <t>TOPO-XL-2</t> cloning of cDNA generated from the serum of an infected pig. The sequences of two independent cDNA amplicons used for the cloning are shown d TOPO-XL-2 cloning of Kos_KSP derived amplicons. In a and c the top bars (light gray) depict differences from the consensus sequence at the nucleotide level, while the lower bars (dark gray) depict differences from the consensus sequence at the amino acid level
    Topo Xl 2 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcr topo xl kit
    Multiple-sequence alignment of amplicons and cDNA clones. Multiple-sequence alignment of cDNA amplicons and cDNA clones ( a and c ) and Kos_KSP amplicons and clones ( b and d ). a In-Fusion cloning of cDNA amplicons generated from the serum of an infected pig. The cDNA amplicons used for the cloning is shown as the first sequence. b In-Fusion cloning of Kos_KSP derived amplicons. c <t>TOPO-XL-2</t> cloning of cDNA generated from the serum of an infected pig. The sequences of two independent cDNA amplicons used for the cloning are shown d TOPO-XL-2 cloning of Kos_KSP derived amplicons. In a and c the top bars (light gray) depict differences from the consensus sequence at the nucleotide level, while the lower bars (dark gray) depict differences from the consensus sequence at the amino acid level
    Pcr Topo Xl Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher topo xl vector kit
    Multiple-sequence alignment of amplicons and cDNA clones. Multiple-sequence alignment of cDNA amplicons and cDNA clones ( a and c ) and Kos_KSP amplicons and clones ( b and d ). a In-Fusion cloning of cDNA amplicons generated from the serum of an infected pig. The cDNA amplicons used for the cloning is shown as the first sequence. b In-Fusion cloning of Kos_KSP derived amplicons. c <t>TOPO-XL-2</t> cloning of cDNA generated from the serum of an infected pig. The sequences of two independent cDNA amplicons used for the cloning are shown d TOPO-XL-2 cloning of Kos_KSP derived amplicons. In a and c the top bars (light gray) depict differences from the consensus sequence at the nucleotide level, while the lower bars (dark gray) depict differences from the consensus sequence at the amino acid level
    Topo Xl Vector Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ta cloning kit
    Multiple-sequence alignment of amplicons and cDNA clones. Multiple-sequence alignment of cDNA amplicons and cDNA clones ( a and c ) and Kos_KSP amplicons and clones ( b and d ). a In-Fusion cloning of cDNA amplicons generated from the serum of an infected pig. The cDNA amplicons used for the cloning is shown as the first sequence. b In-Fusion cloning of Kos_KSP derived amplicons. c <t>TOPO-XL-2</t> cloning of cDNA generated from the serum of an infected pig. The sequences of two independent cDNA amplicons used for the cloning are shown d TOPO-XL-2 cloning of Kos_KSP derived amplicons. In a and c the top bars (light gray) depict differences from the consensus sequence at the nucleotide level, while the lower bars (dark gray) depict differences from the consensus sequence at the amino acid level
    Ta Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 13798 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcr 4 topoxl vector
    Multiple-sequence alignment of amplicons and cDNA clones. Multiple-sequence alignment of cDNA amplicons and cDNA clones ( a and c ) and Kos_KSP amplicons and clones ( b and d ). a In-Fusion cloning of cDNA amplicons generated from the serum of an infected pig. The cDNA amplicons used for the cloning is shown as the first sequence. b In-Fusion cloning of Kos_KSP derived amplicons. c <t>TOPO-XL-2</t> cloning of cDNA generated from the serum of an infected pig. The sequences of two independent cDNA amplicons used for the cloning are shown d TOPO-XL-2 cloning of Kos_KSP derived amplicons. In a and c the top bars (light gray) depict differences from the consensus sequence at the nucleotide level, while the lower bars (dark gray) depict differences from the consensus sequence at the amino acid level
    Pcr 4 Topoxl Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Distinctive P Elements Revealed by Re-screening a Random Sample of P Element Insertion Sites in Natural Populations for Four Genes, Hsp23, Hsp27, Hsrω, and Hsp70 The P element insertion sites were selected from Gene Set I. A plus sign (+) indicates successful PCR amplification with one primer complementary to the focal gene and another complementary to a unique sequence in the P element (top), and thus reports the size and orientation of the P element; a minus sign (−) indicates no amplification. Table S7 provides sequences of these primers. At each insertion site in a population, one to six distinctive P elements segregated; these are designated a–f. For Hsp23, Hsp27, and Hsrω, nine insertion sites shared by two or more natural populations (indicated by boxes) and 17 unique insertion sites were re-screened. Amplicons that share a symbol (filled square [█], filled triangle [▴], filled circle •], etc.) occurred at the same integration site in different populations and were indistinguishable by size or orientation. For Hsp70, a five-copy gene in natural populations [ 17 ], the specific gene of insertion was not determined; thus, each distinctive amplicon (a–f) could represent insertion(s) at the same site in one to five of the Hsp70 genes. For population codes see Figure 1 . ORF, open reading frame.

    Journal: PLoS Genetics

    Article Title: Heat-Shock Promoters: Targets for Evolution by P Transposable Elements in Drosophila

    doi: 10.1371/journal.pgen.0020165

    Figure Lengend Snippet: Distinctive P Elements Revealed by Re-screening a Random Sample of P Element Insertion Sites in Natural Populations for Four Genes, Hsp23, Hsp27, Hsrω, and Hsp70 The P element insertion sites were selected from Gene Set I. A plus sign (+) indicates successful PCR amplification with one primer complementary to the focal gene and another complementary to a unique sequence in the P element (top), and thus reports the size and orientation of the P element; a minus sign (−) indicates no amplification. Table S7 provides sequences of these primers. At each insertion site in a population, one to six distinctive P elements segregated; these are designated a–f. For Hsp23, Hsp27, and Hsrω, nine insertion sites shared by two or more natural populations (indicated by boxes) and 17 unique insertion sites were re-screened. Amplicons that share a symbol (filled square [█], filled triangle [▴], filled circle •], etc.) occurred at the same integration site in different populations and were indistinguishable by size or orientation. For Hsp70, a five-copy gene in natural populations [ 17 ], the specific gene of insertion was not determined; thus, each distinctive amplicon (a–f) could represent insertion(s) at the same site in one to five of the Hsp70 genes. For population codes see Figure 1 . ORF, open reading frame.

    Article Snippet: Topo TA Cloning Kit (Invitrogen, Carlsbad, California, United States) for amplicons fewer than two kilobases and Topo XL PCR Cloning Kit (Invitrogen) for larger fragments were used.

    Techniques: Polymerase Chain Reaction, Amplification, Sequencing

    Multiple-sequence alignment of amplicons and cDNA clones. Multiple-sequence alignment of cDNA amplicons and cDNA clones ( a and c ) and Kos_KSP amplicons and clones ( b and d ). a In-Fusion cloning of cDNA amplicons generated from the serum of an infected pig. The cDNA amplicons used for the cloning is shown as the first sequence. b In-Fusion cloning of Kos_KSP derived amplicons. c TOPO-XL-2 cloning of cDNA generated from the serum of an infected pig. The sequences of two independent cDNA amplicons used for the cloning are shown d TOPO-XL-2 cloning of Kos_KSP derived amplicons. In a and c the top bars (light gray) depict differences from the consensus sequence at the nucleotide level, while the lower bars (dark gray) depict differences from the consensus sequence at the amino acid level

    Journal: BMC Genomics

    Article Title: Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping

    doi: 10.1186/s12864-018-4971-8

    Figure Lengend Snippet: Multiple-sequence alignment of amplicons and cDNA clones. Multiple-sequence alignment of cDNA amplicons and cDNA clones ( a and c ) and Kos_KSP amplicons and clones ( b and d ). a In-Fusion cloning of cDNA amplicons generated from the serum of an infected pig. The cDNA amplicons used for the cloning is shown as the first sequence. b In-Fusion cloning of Kos_KSP derived amplicons. c TOPO-XL-2 cloning of cDNA generated from the serum of an infected pig. The sequences of two independent cDNA amplicons used for the cloning are shown d TOPO-XL-2 cloning of Kos_KSP derived amplicons. In a and c the top bars (light gray) depict differences from the consensus sequence at the nucleotide level, while the lower bars (dark gray) depict differences from the consensus sequence at the amino acid level

    Article Snippet: Cloning of amplicons in pCR XL-2-TOPO vector The cDNA amplicons were also cloned using the TOPO XL-2 kit (Thermo Scientific).

    Techniques: Sequencing, Clone Assay, Generated, Infection, Derivative Assay