top10 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Thermo Fisher top 10 kit
    Top 10 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top 10 kit/product/Thermo Fisher
    Average 94 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    top 10 kit - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    92
    Thermo Fisher top 10
    SDS-PAGE of total proteins (10 μ g per lane) extracted from various lines of E. coli containing expression vectors for soybean GSTs. Lane 1, <t>TOP</t> 10 cells (not transformed); lane 2, molecular mass markers as indicated at left; lane 3, GST3; lane
    Top 10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top 10/product/Thermo Fisher
    Average 92 stars, based on 319 article reviews
    Price from $9.99 to $1999.99
    top 10 - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    Thermo Fisher ecoli top 10
    SDS-PAGE of total proteins (10 μ g per lane) extracted from various lines of E. coli containing expression vectors for soybean GSTs. Lane 1, <t>TOP</t> 10 cells (not transformed); lane 2, molecular mass markers as indicated at left; lane 3, GST3; lane
    Ecoli Top 10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecoli top 10/product/Thermo Fisher
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    ecoli top 10 - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    90
    Thermo Fisher one shotⓡ top 10
    SDS-PAGE of total proteins (10 μ g per lane) extracted from various lines of E. coli containing expression vectors for soybean GSTs. Lane 1, <t>TOP</t> 10 cells (not transformed); lane 2, molecular mass markers as indicated at left; lane 3, GST3; lane
    One Shotⓡ Top 10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one shotⓡ top 10/product/Thermo Fisher
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    one shotⓡ top 10 - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    91
    Fisher Scientific top 10
    SDS-PAGE of total proteins (10 μ g per lane) extracted from various lines of E. coli containing expression vectors for soybean GSTs. Lane 1, <t>TOP</t> 10 cells (not transformed); lane 2, molecular mass markers as indicated at left; lane 3, GST3; lane
    Top 10, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top 10/product/Fisher Scientific
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    top 10 - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    92
    tiangen biotech co top 10
    SDS-PAGE of total proteins (10 μ g per lane) extracted from various lines of E. coli containing expression vectors for soybean GSTs. Lane 1, <t>TOP</t> 10 cells (not transformed); lane 2, molecular mass markers as indicated at left; lane 3, GST3; lane
    Top 10, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top 10/product/tiangen biotech co
    Average 92 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    top 10 - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    TaKaRa top 10
    SDS-PAGE of total proteins (10 μ g per lane) extracted from various lines of E. coli containing expression vectors for soybean GSTs. Lane 1, <t>TOP</t> 10 cells (not transformed); lane 2, molecular mass markers as indicated at left; lane 3, GST3; lane
    Top 10, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top 10/product/TaKaRa
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    top 10 - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    89
    Stratagene escherichia coli top 10
    SDS-PAGE of total proteins (10 μ g per lane) extracted from various lines of E. coli containing expression vectors for soybean GSTs. Lane 1, <t>TOP</t> 10 cells (not transformed); lane 2, molecular mass markers as indicated at left; lane 3, GST3; lane
    Escherichia Coli Top 10, supplied by Stratagene, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli top 10/product/Stratagene
    Average 89 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    escherichia coli top 10 - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    95
    Thermo Fisher electrocompetent top 10 cells
    SDS-PAGE of total proteins (10 μ g per lane) extracted from various lines of E. coli containing expression vectors for soybean GSTs. Lane 1, <t>TOP</t> 10 cells (not transformed); lane 2, molecular mass markers as indicated at left; lane 3, GST3; lane
    Electrocompetent Top 10 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrocompetent top 10 cells/product/Thermo Fisher
    Average 95 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    electrocompetent top 10 cells - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    85
    Thermo Fisher minipreparations top 10 cells
    Side-by-side comparison of microbial hosts for their ability to maintain the same plasmid. ( A ) Universal deletion in E.coli <t>Top</t> 10 cells. Plasmids re-isolated from TOP 10 clones transformed with the H4#4 plasmid are deleted. Each was XbaI and PvuII digested. H4#4 DNA (also cut with XbaI and PvuII after a phi-29 amplification) is loaded adjacent to the marker lane. Note, a different 100 bp ladder was used here (New England Biolabs) which has an intense 500 bp rather than 600 bp band as in previous figures. ( B ) Instability of the H4#4 plasmid in E.coli SURE cells. Plasmid DNA from individual SURE H4#4 transformants is a mixture of deleted and apparently non-deleted forms despite the lack of a functional SbcCD nuclease. The gel image was cut to remove one lane. ( C ) Stability of H4#4 plasmid in wild-type yeast. Phi-29 amplified <t>minipreparations</t> of DNA from random wild-type yeast clones transformed with H4#4 DNA were digested with XbaI and PvuII. Full-length inserts are observed. ( D ) Phi-29 amplified minipreparations of DNA from random sae2 yeast clones transformed and analyzed as in (C). In A–D, dots mark samples from colonies that were re-streaked as described in the text.
    Minipreparations Top 10 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minipreparations top 10 cells/product/Thermo Fisher
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    minipreparations top 10 cells - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    96
    Thermo Fisher top 10 competent cells
    Side-by-side comparison of microbial hosts for their ability to maintain the same plasmid. ( A ) Universal deletion in E.coli <t>Top</t> 10 cells. Plasmids re-isolated from TOP 10 clones transformed with the H4#4 plasmid are deleted. Each was XbaI and PvuII digested. H4#4 DNA (also cut with XbaI and PvuII after a phi-29 amplification) is loaded adjacent to the marker lane. Note, a different 100 bp ladder was used here (New England Biolabs) which has an intense 500 bp rather than 600 bp band as in previous figures. ( B ) Instability of the H4#4 plasmid in E.coli SURE cells. Plasmid DNA from individual SURE H4#4 transformants is a mixture of deleted and apparently non-deleted forms despite the lack of a functional SbcCD nuclease. The gel image was cut to remove one lane. ( C ) Stability of H4#4 plasmid in wild-type yeast. Phi-29 amplified <t>minipreparations</t> of DNA from random wild-type yeast clones transformed with H4#4 DNA were digested with XbaI and PvuII. Full-length inserts are observed. ( D ) Phi-29 amplified minipreparations of DNA from random sae2 yeast clones transformed and analyzed as in (C). In A–D, dots mark samples from colonies that were re-streaked as described in the text.
    Top 10 Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top 10 competent cells/product/Thermo Fisher
    Average 96 stars, based on 104 article reviews
    Price from $9.99 to $1999.99
    top 10 competent cells - by Bioz Stars, 2020-08
    96/100 stars
      Buy from Supplier

    99
    Thermo Fisher shot top 10 cells
    Side-by-side comparison of microbial hosts for their ability to maintain the same plasmid. ( A ) Universal deletion in E.coli <t>Top</t> 10 cells. Plasmids re-isolated from TOP 10 clones transformed with the H4#4 plasmid are deleted. Each was XbaI and PvuII digested. H4#4 DNA (also cut with XbaI and PvuII after a phi-29 amplification) is loaded adjacent to the marker lane. Note, a different 100 bp ladder was used here (New England Biolabs) which has an intense 500 bp rather than 600 bp band as in previous figures. ( B ) Instability of the H4#4 plasmid in E.coli SURE cells. Plasmid DNA from individual SURE H4#4 transformants is a mixture of deleted and apparently non-deleted forms despite the lack of a functional SbcCD nuclease. The gel image was cut to remove one lane. ( C ) Stability of H4#4 plasmid in wild-type yeast. Phi-29 amplified <t>minipreparations</t> of DNA from random wild-type yeast clones transformed with H4#4 DNA were digested with XbaI and PvuII. Full-length inserts are observed. ( D ) Phi-29 amplified minipreparations of DNA from random sae2 yeast clones transformed and analyzed as in (C). In A–D, dots mark samples from colonies that were re-streaked as described in the text.
    Shot Top 10 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shot top 10 cells/product/Thermo Fisher
    Average 99 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    shot top 10 cells - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    85
    Thermo Fisher competent escherichia coli top 10
    Side-by-side comparison of microbial hosts for their ability to maintain the same plasmid. ( A ) Universal deletion in E.coli <t>Top</t> 10 cells. Plasmids re-isolated from TOP 10 clones transformed with the H4#4 plasmid are deleted. Each was XbaI and PvuII digested. H4#4 DNA (also cut with XbaI and PvuII after a phi-29 amplification) is loaded adjacent to the marker lane. Note, a different 100 bp ladder was used here (New England Biolabs) which has an intense 500 bp rather than 600 bp band as in previous figures. ( B ) Instability of the H4#4 plasmid in E.coli SURE cells. Plasmid DNA from individual SURE H4#4 transformants is a mixture of deleted and apparently non-deleted forms despite the lack of a functional SbcCD nuclease. The gel image was cut to remove one lane. ( C ) Stability of H4#4 plasmid in wild-type yeast. Phi-29 amplified <t>minipreparations</t> of DNA from random wild-type yeast clones transformed with H4#4 DNA were digested with XbaI and PvuII. Full-length inserts are observed. ( D ) Phi-29 amplified minipreparations of DNA from random sae2 yeast clones transformed and analyzed as in (C). In A–D, dots mark samples from colonies that were re-streaked as described in the text.
    Competent Escherichia Coli Top 10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent escherichia coli top 10/product/Thermo Fisher
    Average 85 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    competent escherichia coli top 10 - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    85
    Thermo Fisher oneshot top 10 cells
    Side-by-side comparison of microbial hosts for their ability to maintain the same plasmid. ( A ) Universal deletion in E.coli <t>Top</t> 10 cells. Plasmids re-isolated from TOP 10 clones transformed with the H4#4 plasmid are deleted. Each was XbaI and PvuII digested. H4#4 DNA (also cut with XbaI and PvuII after a phi-29 amplification) is loaded adjacent to the marker lane. Note, a different 100 bp ladder was used here (New England Biolabs) which has an intense 500 bp rather than 600 bp band as in previous figures. ( B ) Instability of the H4#4 plasmid in E.coli SURE cells. Plasmid DNA from individual SURE H4#4 transformants is a mixture of deleted and apparently non-deleted forms despite the lack of a functional SbcCD nuclease. The gel image was cut to remove one lane. ( C ) Stability of H4#4 plasmid in wild-type yeast. Phi-29 amplified <t>minipreparations</t> of DNA from random wild-type yeast clones transformed with H4#4 DNA were digested with XbaI and PvuII. Full-length inserts are observed. ( D ) Phi-29 amplified minipreparations of DNA from random sae2 yeast clones transformed and analyzed as in (C). In A–D, dots mark samples from colonies that were re-streaked as described in the text.
    Oneshot Top 10 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oneshot top 10 cells/product/Thermo Fisher
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    oneshot top 10 cells - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    SDS-PAGE of total proteins (10 μ g per lane) extracted from various lines of E. coli containing expression vectors for soybean GSTs. Lane 1, TOP 10 cells (not transformed); lane 2, molecular mass markers as indicated at left; lane 3, GST3; lane

    Journal: Plant Physiology

    Article Title: Physiological Roles of Glutathione S-Transferases in Soybean Root Nodules 1-Transferases in Soybean Root Nodules 1 [C]-Transferases in Soybean Root Nodules 1 [C] [W]-Transferases in Soybean Root Nodules 1 [C] [W] [OA]

    doi: 10.1104/pp.109.136630

    Figure Lengend Snippet: SDS-PAGE of total proteins (10 μ g per lane) extracted from various lines of E. coli containing expression vectors for soybean GSTs. Lane 1, TOP 10 cells (not transformed); lane 2, molecular mass markers as indicated at left; lane 3, GST3; lane

    Article Snippet: These were amplified with PCR, subcloned into the expression vector pTrcHis-TOPO, and transformed into TOP 10 cells (Invitrogen).

    Techniques: SDS Page, Expressing, Transformation Assay

    Regulated transcription of asa . ( A ) Promoter activity of the cloned putative promoter region (black letters in Fig. 4A ) was measured as fluorescence intensity of E. coli Top-10 + pProbe-NT:: asa P (OD 600 = 0.8) after growth in LB (grey), in LB + 450 mM NaCl (blue) or in LB + 10 mM L-arginine (orange); NC I: negative control using the terminator region of the gene EDL933_1236 (violet letters in Fig. 4A ), NC II: pProbe-NT without insert. The mean values and standard deviations of all three replicates are shown here. ( B ) RT-qPCR threshold cycles of asa normalized to the 16S rRNA gene (∆cq). Lower ∆cq values indicate higher amounts of mRNA and vice versa . EHEC was grown in LB medium (left columns) or LB + 450 mM NaCl (right columns). Approximately 10 8 cells were harvested for total RNA extraction either at exponential phase (OD 600 = 0.2–0.3, blue) or at late exponential phase (OD 600 = 0.7 – 0.8, orange). The RNA was reverse transcribed into cDNA and quantified using RT-qPCR. The mean values and standard deviations of all three replicates are shown here.

    Journal: Scientific Reports

    Article Title: The novel EHEC gene asa overlaps the TEGT transporter gene in antisense and is regulated by NaCl and growth phase

    doi: 10.1038/s41598-018-35756-y

    Figure Lengend Snippet: Regulated transcription of asa . ( A ) Promoter activity of the cloned putative promoter region (black letters in Fig. 4A ) was measured as fluorescence intensity of E. coli Top-10 + pProbe-NT:: asa P (OD 600 = 0.8) after growth in LB (grey), in LB + 450 mM NaCl (blue) or in LB + 10 mM L-arginine (orange); NC I: negative control using the terminator region of the gene EDL933_1236 (violet letters in Fig. 4A ), NC II: pProbe-NT without insert. The mean values and standard deviations of all three replicates are shown here. ( B ) RT-qPCR threshold cycles of asa normalized to the 16S rRNA gene (∆cq). Lower ∆cq values indicate higher amounts of mRNA and vice versa . EHEC was grown in LB medium (left columns) or LB + 450 mM NaCl (right columns). Approximately 10 8 cells were harvested for total RNA extraction either at exponential phase (OD 600 = 0.2–0.3, blue) or at late exponential phase (OD 600 = 0.7 – 0.8, orange). The RNA was reverse transcribed into cDNA and quantified using RT-qPCR. The mean values and standard deviations of all three replicates are shown here.

    Article Snippet: List of bacteria and incubation conditions The following bacterial strains were used: E. coli O157:H7 strain EDL933 (Collection de l’Institute Pasteur: CIP 106327, GenBank accession number CP008957.1), E. coli O157:H7 strain Sakai (Weihenstephan strain collection WS 4518, GenBank accession number NC_002695.1), E. coli LF82 (kindly provided by R. Balfour Sartor, GenBank accession number NC_011993.1), E. coli TOP-10 (Invitrogen, Paisley, UK).

    Techniques: Activity Assay, Clone Assay, Fluorescence, Negative Control, Quantitative RT-PCR, RNA Extraction

    Side-by-side comparison of microbial hosts for their ability to maintain the same plasmid. ( A ) Universal deletion in E.coli Top 10 cells. Plasmids re-isolated from TOP 10 clones transformed with the H4#4 plasmid are deleted. Each was XbaI and PvuII digested. H4#4 DNA (also cut with XbaI and PvuII after a phi-29 amplification) is loaded adjacent to the marker lane. Note, a different 100 bp ladder was used here (New England Biolabs) which has an intense 500 bp rather than 600 bp band as in previous figures. ( B ) Instability of the H4#4 plasmid in E.coli SURE cells. Plasmid DNA from individual SURE H4#4 transformants is a mixture of deleted and apparently non-deleted forms despite the lack of a functional SbcCD nuclease. The gel image was cut to remove one lane. ( C ) Stability of H4#4 plasmid in wild-type yeast. Phi-29 amplified minipreparations of DNA from random wild-type yeast clones transformed with H4#4 DNA were digested with XbaI and PvuII. Full-length inserts are observed. ( D ) Phi-29 amplified minipreparations of DNA from random sae2 yeast clones transformed and analyzed as in (C). In A–D, dots mark samples from colonies that were re-streaked as described in the text.

    Journal: Nucleic Acids Research

    Article Title: New approaches to the analysis of palindromic sequences from the human genome: evolution and polymorphism of an intronic site at the NF1 locus

    doi: 10.1093/nar/gni189

    Figure Lengend Snippet: Side-by-side comparison of microbial hosts for their ability to maintain the same plasmid. ( A ) Universal deletion in E.coli Top 10 cells. Plasmids re-isolated from TOP 10 clones transformed with the H4#4 plasmid are deleted. Each was XbaI and PvuII digested. H4#4 DNA (also cut with XbaI and PvuII after a phi-29 amplification) is loaded adjacent to the marker lane. Note, a different 100 bp ladder was used here (New England Biolabs) which has an intense 500 bp rather than 600 bp band as in previous figures. ( B ) Instability of the H4#4 plasmid in E.coli SURE cells. Plasmid DNA from individual SURE H4#4 transformants is a mixture of deleted and apparently non-deleted forms despite the lack of a functional SbcCD nuclease. The gel image was cut to remove one lane. ( C ) Stability of H4#4 plasmid in wild-type yeast. Phi-29 amplified minipreparations of DNA from random wild-type yeast clones transformed with H4#4 DNA were digested with XbaI and PvuII. Full-length inserts are observed. ( D ) Phi-29 amplified minipreparations of DNA from random sae2 yeast clones transformed and analyzed as in (C). In A–D, dots mark samples from colonies that were re-streaked as described in the text.

    Article Snippet: Bacterial strains, transformation and minipreparations TOP 10 cells (Invitrogen) are F-mcrA Δ(mrr-hsdRMS-mcrBC) f80lacZ ΔM15 ΔlacX74 deoR recA1 ara Δ139 Δ(ara-leu)7697 galUΔgalK rpsL (StrR ) endA1 nupG .

    Techniques: Plasmid Preparation, Isolation, Clone Assay, Transformation Assay, Amplification, Marker, Functional Assay