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  • 99
    Thermo Fisher todd hewitt broth
    Sialidase and hyaluronidase production by the wild-type, mutant and complemented strains. Quantitative assays were carried out to determine the relative amount of extracellular enzyme production by the wild-type, mutant and complemented strains at time of inoculation and 4 h and 8 h post-inoculation (corresponding to exponential and stationary growth phases, respectively). Sialidase assays were performed using culture supernatants from cultures grown in <t>Todd-Hewitt</t> broth (A). Hyaluronidase activity was determined using the cell lysates from cultures grown in TPYG (B). All results are given as the average of three biological replicates (± SEM) for sialidase assays and six biological replicates for hyaluronidase assays; the asterisk (*) denotes a statistically significant difference ( p ≤0.05) as determined by the student’s t-test.
    Todd Hewitt Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 923 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/todd hewitt broth/product/Thermo Fisher
    Average 99 stars, based on 923 article reviews
    Price from $9.99 to $1999.99
    todd hewitt broth - by Bioz Stars, 2020-07
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    95
    Millipore todd hewitt broth
    Extracellular enzyme production in the isogenic revR strains. Quantitative assays were carried out to determine the relative amount of enzyme activity in 4-h cultures grown in TPYG medium or <t>Todd-Hewitt</t> broth (for sialidase assays only). Enzyme activity
    Todd Hewitt Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/todd hewitt broth/product/Millipore
    Average 95 stars, based on 158 article reviews
    Price from $9.99 to $1999.99
    todd hewitt broth - by Bioz Stars, 2020-07
    95/100 stars
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    90
    Thermo Fisher todd hewitt oxoid broth
    Extracellular enzyme production in the isogenic revR strains. Quantitative assays were carried out to determine the relative amount of enzyme activity in 4-h cultures grown in TPYG medium or <t>Todd-Hewitt</t> broth (for sialidase assays only). Enzyme activity
    Todd Hewitt Oxoid Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/todd hewitt oxoid broth/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    todd hewitt oxoid broth - by Bioz Stars, 2020-07
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    93
    Difco todd hewitt broth
    Effect of Dectin-2 deficiency on IL-12p40 production by BD-DCs upon stimulation with S. pneumoniae . BM-DCs from WT mice (open columns) or Dectin-2KO mice (solid columns) were cultured with live S. pneumoniae ( a ); lysates from S. pneumoniae or sham-operated PBS (Sham) ( b ); S. pneumoniae culture supernatant or <t>Todd-Hewitt</t> broth (THB) ( c ); or LPS (1 μg/ml), CpG (1 μg/ml) or mannan (3 mg/ml) for 24 h. IL-12p40 concentrations in the culture supernatants were measured using ELISA. Each column shows the mean ± SD of triplicate culture. Similar results were obtained in three independent experiments. MOI, multiplicity of infection. *, p
    Todd Hewitt Broth, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 1597 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/todd hewitt broth/product/Difco
    Average 93 stars, based on 1597 article reviews
    Price from $9.99 to $1999.99
    todd hewitt broth - by Bioz Stars, 2020-07
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      Buy from Supplier

    Image Search Results


    Sialidase and hyaluronidase production by the wild-type, mutant and complemented strains. Quantitative assays were carried out to determine the relative amount of extracellular enzyme production by the wild-type, mutant and complemented strains at time of inoculation and 4 h and 8 h post-inoculation (corresponding to exponential and stationary growth phases, respectively). Sialidase assays were performed using culture supernatants from cultures grown in Todd-Hewitt broth (A). Hyaluronidase activity was determined using the cell lysates from cultures grown in TPYG (B). All results are given as the average of three biological replicates (± SEM) for sialidase assays and six biological replicates for hyaluronidase assays; the asterisk (*) denotes a statistically significant difference ( p ≤0.05) as determined by the student’s t-test.

    Journal: PLoS ONE

    Article Title: Regulation of Sialidase Production in Clostridium perfringens by the Orphan Sensor Histidine Kinase ReeS

    doi: 10.1371/journal.pone.0073525

    Figure Lengend Snippet: Sialidase and hyaluronidase production by the wild-type, mutant and complemented strains. Quantitative assays were carried out to determine the relative amount of extracellular enzyme production by the wild-type, mutant and complemented strains at time of inoculation and 4 h and 8 h post-inoculation (corresponding to exponential and stationary growth phases, respectively). Sialidase assays were performed using culture supernatants from cultures grown in Todd-Hewitt broth (A). Hyaluronidase activity was determined using the cell lysates from cultures grown in TPYG (B). All results are given as the average of three biological replicates (± SEM) for sialidase assays and six biological replicates for hyaluronidase assays; the asterisk (*) denotes a statistically significant difference ( p ≤0.05) as determined by the student’s t-test.

    Article Snippet: Hyaluronidase and Sialidase Assays C. perfringens cultures were grown in either TPYG or Todd-Hewitt broth (Oxoid) supplemented with 0.1% (w/v) sodium thioglycolate and 0.37% (w/v) glucose after autoclaving.

    Techniques: Mutagenesis, Activity Assay

    Growth of S. dysgalactiae subsp. equisimilis in the presence of LL-37 and DrsG. S. dysgalactiae subsp. equisimilis MD985 ( drsG negative) was grown in the presence of LL-37 and/or DrsGL (A), DrsGS (B), or SIC (C). After a 2.5-h incubation, the bacteria were recovered, plated onto Todd-Hewitt agar, and incubated overnight. The percentage of growth compared to that of controls was determined for each individual assay. The results presented are the means for three independent experiments.

    Journal: Infection and Immunity

    Article Title: DrsG from Streptococcus dysgalactiae subsp. equisimilis Inhibits the Antimicrobial Peptide LL-37

    doi: 10.1128/IAI.01411-13

    Figure Lengend Snippet: Growth of S. dysgalactiae subsp. equisimilis in the presence of LL-37 and DrsG. S. dysgalactiae subsp. equisimilis MD985 ( drsG negative) was grown in the presence of LL-37 and/or DrsGL (A), DrsGS (B), or SIC (C). After a 2.5-h incubation, the bacteria were recovered, plated onto Todd-Hewitt agar, and incubated overnight. The percentage of growth compared to that of controls was determined for each individual assay. The results presented are the means for three independent experiments.

    Article Snippet: Unless otherwise specified, S. dysgalactiae subsp. equisimilis and S. pyogenes were grown in Todd-Hewitt broth (THB), Todd-Hewitt agar (Oxoid), or Columbia agar (CBA; Oxoid) supplemented with 5% defibrinated horse blood.

    Techniques: Incubation

    Extracellular enzyme production in the isogenic revR strains. Quantitative assays were carried out to determine the relative amount of enzyme activity in 4-h cultures grown in TPYG medium or Todd-Hewitt broth (for sialidase assays only). Enzyme activity

    Journal: Infection and Immunity

    Article Title: Regulation of Virulence by the RevR Response Regulator in Clostridium perfringens ▿ ▿ †

    doi: 10.1128/IAI.00060-11

    Figure Lengend Snippet: Extracellular enzyme production in the isogenic revR strains. Quantitative assays were carried out to determine the relative amount of enzyme activity in 4-h cultures grown in TPYG medium or Todd-Hewitt broth (for sialidase assays only). Enzyme activity

    Article Snippet: For sialidase assays, culture supernatants from Todd-Hewitt broth cultures were collected by centrifugation at 5800 × g for 10 min and concentrated 20-fold using Amicon Ultra centrifugal filter devices (Millipore) with a nominal cutoff of 30 kDa.

    Techniques: Activity Assay

    Sialidase and hyaluronidase production by the wild-type, mutant and complemented strains. Quantitative assays were carried out to determine the relative amount of extracellular enzyme production by the wild-type, mutant and complemented strains at time of inoculation and 4 h and 8 h post-inoculation (corresponding to exponential and stationary growth phases, respectively). Sialidase assays were performed using culture supernatants from cultures grown in Todd-Hewitt broth (A). Hyaluronidase activity was determined using the cell lysates from cultures grown in TPYG (B). All results are given as the average of three biological replicates (± SEM) for sialidase assays and six biological replicates for hyaluronidase assays; the asterisk (*) denotes a statistically significant difference ( p ≤0.05) as determined by the student’s t-test.

    Journal: PLoS ONE

    Article Title: Regulation of Sialidase Production in Clostridium perfringens by the Orphan Sensor Histidine Kinase ReeS

    doi: 10.1371/journal.pone.0073525

    Figure Lengend Snippet: Sialidase and hyaluronidase production by the wild-type, mutant and complemented strains. Quantitative assays were carried out to determine the relative amount of extracellular enzyme production by the wild-type, mutant and complemented strains at time of inoculation and 4 h and 8 h post-inoculation (corresponding to exponential and stationary growth phases, respectively). Sialidase assays were performed using culture supernatants from cultures grown in Todd-Hewitt broth (A). Hyaluronidase activity was determined using the cell lysates from cultures grown in TPYG (B). All results are given as the average of three biological replicates (± SEM) for sialidase assays and six biological replicates for hyaluronidase assays; the asterisk (*) denotes a statistically significant difference ( p ≤0.05) as determined by the student’s t-test.

    Article Snippet: Todd-Hewitt broth culture supernatants, derived from three independent biological replicates, were concentrated using Amicon ultra centrifugal devices (Millipore) with a nominal weight cutoff of 30 kDa.

    Techniques: Mutagenesis, Activity Assay

    Effect of Dectin-2 deficiency on IL-12p40 production by BD-DCs upon stimulation with S. pneumoniae . BM-DCs from WT mice (open columns) or Dectin-2KO mice (solid columns) were cultured with live S. pneumoniae ( a ); lysates from S. pneumoniae or sham-operated PBS (Sham) ( b ); S. pneumoniae culture supernatant or Todd-Hewitt broth (THB) ( c ); or LPS (1 μg/ml), CpG (1 μg/ml) or mannan (3 mg/ml) for 24 h. IL-12p40 concentrations in the culture supernatants were measured using ELISA. Each column shows the mean ± SD of triplicate culture. Similar results were obtained in three independent experiments. MOI, multiplicity of infection. *, p

    Journal: BMC Immunology

    Article Title: Dectin-2-dependent host defense in mice infected with serotype 3 Streptococcus pneumoniae

    doi: 10.1186/s12865-015-0139-3

    Figure Lengend Snippet: Effect of Dectin-2 deficiency on IL-12p40 production by BD-DCs upon stimulation with S. pneumoniae . BM-DCs from WT mice (open columns) or Dectin-2KO mice (solid columns) were cultured with live S. pneumoniae ( a ); lysates from S. pneumoniae or sham-operated PBS (Sham) ( b ); S. pneumoniae culture supernatant or Todd-Hewitt broth (THB) ( c ); or LPS (1 μg/ml), CpG (1 μg/ml) or mannan (3 mg/ml) for 24 h. IL-12p40 concentrations in the culture supernatants were measured using ELISA. Each column shows the mean ± SD of triplicate culture. Similar results were obtained in three independent experiments. MOI, multiplicity of infection. *, p

    Article Snippet: Todd-Hewitt broth incubated without S. pneumoniae was used as a control.

    Techniques: Mouse Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Infection