tnfsf11 rankl picokine elisa kit Search Results


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    Boster Bio mouse tnfsf11 rankl picokine elisa kit
    Mouse Tnfsf11 Rankl Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse tnfsf11 rankl picokine elisa kit/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse tnfsf11 rankl picokine elisa kit - by Bioz Stars, 2022-10
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    94
    Boster Bio tnfsf11 rankl picokine elisa kit
    Adipocytes are the principal source for <t>RANKL</t> after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression. (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre;IFT20 f/f mice and controls. Scale bar, 200 μm. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre;IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre;IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre;IFT20 f/f mice and controls. Scale bars, 1 mm. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre;IFT20 f/f mice and controls. (H) Representative H E-stained image of femurs from 1-month-old Adipoq-Cre;IFT20 f/f mice and controls. Scale bars, 200 μm. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre;IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre;IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P
    Tnfsf11 Rankl Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnfsf11 rankl picokine elisa kit/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnfsf11 rankl picokine elisa kit - by Bioz Stars, 2022-10
    94/100 stars
      Buy from Supplier

    94
    Boster Bio elisa kit
    Influence of <t>Syndecan-1</t> deficiency on serum concentration of RANKL and OPG and bone structure in mice during aging. A, B, C : Serum was collected from female mice (WT (all), Syndecan-1 KO (only in B , C )) of 4, 12 and 18 month of age and concentration of Syndecan-1 ( A ), RANKL ( B ) and OPG ( C ) was determined by <t>ELISA.</t> Data are displayed as mean ± SD, Kruskal-Wallis test with Dunn’s post hoc test, n =9-13 per group. *p
    Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kit/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa kit - by Bioz Stars, 2022-10
    94/100 stars
      Buy from Supplier

    Image Search Results


    Adipocytes are the principal source for RANKL after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression. (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre;IFT20 f/f mice and controls. Scale bar, 200 μm. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre;IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre;IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre;IFT20 f/f mice and controls. Scale bars, 1 mm. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre;IFT20 f/f mice and controls. (H) Representative H E-stained image of femurs from 1-month-old Adipoq-Cre;IFT20 f/f mice and controls. Scale bars, 200 μm. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre;IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre;IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P

    Journal: bioRxiv

    Article Title: IFT20 governs mesenchymal stem cell fate through positively regulating TGF-β-Smad2/3-Glut1 signaling mediated glucose metabolism

    doi: 10.1101/2022.02.28.482266

    Figure Lengend Snippet: Adipocytes are the principal source for RANKL after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression. (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre;IFT20 f/f mice and controls. Scale bar, 200 μm. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre;IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre;IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre;IFT20 f/f mice and controls. Scale bars, 1 mm. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre;IFT20 f/f mice and controls. (H) Representative H E-stained image of femurs from 1-month-old Adipoq-Cre;IFT20 f/f mice and controls. Scale bars, 200 μm. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre;IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre;IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P

    Article Snippet: Serum levels of OCN, OPG, RANKL and insulin were measured by mouse Osteocalcin ELISA Kit (BioVision), OPG ELISA Kit (Boster Biological Technology), TNFSF11/RANKL PicoKine ELISA Kit (Boster Biological Technology) and Ultra-Sensitive Insulin ELISA Kit (Crystal Chem), respectively, according to the manufacturer’s instructions.

    Techniques: Expressing, Staining, Mouse Assay, Quantitative RT-PCR, Micro-CT

    Influence of Syndecan-1 deficiency on serum concentration of RANKL and OPG and bone structure in mice during aging. A, B, C : Serum was collected from female mice (WT (all), Syndecan-1 KO (only in B , C )) of 4, 12 and 18 month of age and concentration of Syndecan-1 ( A ), RANKL ( B ) and OPG ( C ) was determined by ELISA. Data are displayed as mean ± SD, Kruskal-Wallis test with Dunn’s post hoc test, n =9-13 per group. *p

    Journal: bioRxiv

    Article Title: The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis

    doi: 10.1101/852590

    Figure Lengend Snippet: Influence of Syndecan-1 deficiency on serum concentration of RANKL and OPG and bone structure in mice during aging. A, B, C : Serum was collected from female mice (WT (all), Syndecan-1 KO (only in B , C )) of 4, 12 and 18 month of age and concentration of Syndecan-1 ( A ), RANKL ( B ) and OPG ( C ) was determined by ELISA. Data are displayed as mean ± SD, Kruskal-Wallis test with Dunn’s post hoc test, n =9-13 per group. *p

    Article Snippet: The concentration of Syndecan-1, RANKL and OPG was determined using an ELISA Kit (Syndecan-1: Boster; RANKL/OPG: R & D).

    Techniques: Concentration Assay, Mouse Assay, Enzyme-linked Immunosorbent Assay

    Syndecan-1, RANKL and OPG expression was analysed after stimulation of osteoblastic cells with DM or DM+ medium (day 7). A : mRNA expression of Syndecan-1 (in WT cells) was determined by real time PCR. B, C : The concentration of soluble Syndecan-1 protein in the supernatant ( B ) and cell layer ( C ) of cells (WT) was analysed by ELISA. Experiments were performed in triplicates. Mann-Whitney-U test, *p

    Journal: bioRxiv

    Article Title: The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis

    doi: 10.1101/852590

    Figure Lengend Snippet: Syndecan-1, RANKL and OPG expression was analysed after stimulation of osteoblastic cells with DM or DM+ medium (day 7). A : mRNA expression of Syndecan-1 (in WT cells) was determined by real time PCR. B, C : The concentration of soluble Syndecan-1 protein in the supernatant ( B ) and cell layer ( C ) of cells (WT) was analysed by ELISA. Experiments were performed in triplicates. Mann-Whitney-U test, *p

    Article Snippet: The concentration of Syndecan-1, RANKL and OPG was determined using an ELISA Kit (Syndecan-1: Boster; RANKL/OPG: R & D).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY