Journal: American Journal of Translational Research
Article Title: The expression of PD-1 and LAG-3 in periapical lesions
Figure Lengend Snippet: The expression of TNF-α, CD3, CD4 and CD8 in periapical lesions. Inflammatory response of healthy oral mucosa (n=3), periapical granuloma (n=14) and radicular cyst (n=13) was detected by immunohistochemical staining. A, F, K. HE staining of healthy oral mucosa, periapical granuloma and radicular cyst. B, G, L. TNF-α significantly overexpressed in periapical granuloma, and mildly decreased in radicular cyst. In the healthy oral mucosa, TNF-α were scarcely detected. C, H, M. The infiltration of total T cells was detected by CD3 immunostaining. Similar to the expression of TNF-α, CD3 + T cells accumulated in granuloma and reduced in radicular cyst, while it is seldom observed in healthy oral mucosa. D, I, N, E, J, O. CD8 and CD4 were stained, the positive cells accumulated within periapical granuloma and radicular cyst, while CD8 + and CD4 + cells in healthy mucosa were barely detected. Scale bar = 50 μm.
Article Snippet: Briefly, sections were incubated with first antibodies overnight at 4°C.The antibodies included: anti-CD4 (Abcam, Cambridge, MA, USA), anti-CD8 (Abcam, Cambridge, MA, USA), anti-CD3 (Abcam, Cambridge, MA, USA), anti-LAG-3 (Cell Signaling Technology, Danvers, MA, USA), anti-tumor necrosis factor (TNF)-α (Cell Signaling Technology), anti-PD-1 (Cell Signaling Technology).
Techniques: Expressing, Immunohistochemistry, Staining, Immunostaining