tnfα Biolegend Search Results


pe anti lfa1  (Miltenyi Biotec)
90
Miltenyi Biotec pe anti lfa1
Pe Anti Lfa1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pe anti lfa1/product/Miltenyi Biotec
Average 90 stars, based on 1 article reviews
pe anti lfa1 - by Bioz Stars, 2026-02
90/100 stars
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human tnfα  (Revvity)
90
Revvity human tnfα
(A) Schematic representation of the experimental approach, comparing dendritic cell (DC) with tolerogenic dendritic cell (tolDC) differentiation. (B) DC and tolDC were cocultured with CD8+ cells for 5 days. The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. In the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)). (C) IL-10, <t>TNFα,</t> <t>IL-12p70</t> and IL1-β production of DC and tolDC, after 5 days of differentiation and 24 h of LPS (10 ng/μL) and IFNg (20 ng/μL) stimuli. P-values of paired t-tests are shown. (D) Box-plots of CD80, CD83, CD86 and HLA-DR surface expression (Median Fluorescence Intensity) in DCs and tolDCs in steady-state or stimulated with LPS (10 ng/μL) and IFNg (20 ng/μL) (ns p > 0.05, ** p < 0.01, *** p ≤ 0.001). (E) Gene expression heatmap of differentially expressed genes comparing tolDCs with DCs and also displaying the gene expression values of the precursor cell type (MO) (logFC > 0.5, FDR < 0.05). Scaled fluorescence values of expression arrays are shown, ranging from -2 (lower gene expression, green) to +2 (higher gene expression, orange). (F) Gene ontology (GO) over-representation of GO Biological Process categories. Fold change of tolDC induced genes over background and -log10(FDR) of Fisher’s exact tests are shown. (G) Discriminant regulon expression analysis (DoRothEA) of tolDC compared with DC. Only transcription factors with FDR < 0.05 are shown. NES and logFC of transcription factor expression are depicted. (H) T-distributed stochastic neighbor embedding (t-SNE) plot of the aggregated and batch-corrected gene expression data from our study (MO, DC and tolDC) and two additional public datasets (GSE40484 (moMAC, moDC, cDC2, CM (Classical MOs) and NCM (Non-Classical MOs) and GSE99056 (M-MAC (M2 Macrophages) and GM-MAC (M1 Macrophages)). The 4 different groups obtained using k-means clustering are represented with grey ellipses of multivariate t-distributions.
Human Tnfα, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human tnfα/product/Revvity
Average 90 stars, based on 1 article reviews
human tnfα - by Bioz Stars, 2026-02
90/100 stars
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pe-conjugated anti-ccr6  (Becton Dickinson)
90
Becton Dickinson pe-conjugated anti-ccr6
(A) Schematic representation of the experimental approach, comparing dendritic cell (DC) with tolerogenic dendritic cell (tolDC) differentiation. (B) DC and tolDC were cocultured with CD8+ cells for 5 days. The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. In the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)). (C) IL-10, <t>TNFα,</t> <t>IL-12p70</t> and IL1-β production of DC and tolDC, after 5 days of differentiation and 24 h of LPS (10 ng/μL) and IFNg (20 ng/μL) stimuli. P-values of paired t-tests are shown. (D) Box-plots of CD80, CD83, CD86 and HLA-DR surface expression (Median Fluorescence Intensity) in DCs and tolDCs in steady-state or stimulated with LPS (10 ng/μL) and IFNg (20 ng/μL) (ns p > 0.05, ** p < 0.01, *** p ≤ 0.001). (E) Gene expression heatmap of differentially expressed genes comparing tolDCs with DCs and also displaying the gene expression values of the precursor cell type (MO) (logFC > 0.5, FDR < 0.05). Scaled fluorescence values of expression arrays are shown, ranging from -2 (lower gene expression, green) to +2 (higher gene expression, orange). (F) Gene ontology (GO) over-representation of GO Biological Process categories. Fold change of tolDC induced genes over background and -log10(FDR) of Fisher’s exact tests are shown. (G) Discriminant regulon expression analysis (DoRothEA) of tolDC compared with DC. Only transcription factors with FDR < 0.05 are shown. NES and logFC of transcription factor expression are depicted. (H) T-distributed stochastic neighbor embedding (t-SNE) plot of the aggregated and batch-corrected gene expression data from our study (MO, DC and tolDC) and two additional public datasets (GSE40484 (moMAC, moDC, cDC2, CM (Classical MOs) and NCM (Non-Classical MOs) and GSE99056 (M-MAC (M2 Macrophages) and GM-MAC (M1 Macrophages)). The 4 different groups obtained using k-means clustering are represented with grey ellipses of multivariate t-distributions.
Pe Conjugated Anti Ccr6, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pe-conjugated anti-ccr6/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
pe-conjugated anti-ccr6 - by Bioz Stars, 2026-02
90/100 stars
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foxp3-efluor 450 ebioscience  (Thermo Fisher)
90
Thermo Fisher foxp3-efluor 450 ebioscience
(A) Schematic representation of the experimental approach, comparing dendritic cell (DC) with tolerogenic dendritic cell (tolDC) differentiation. (B) DC and tolDC were cocultured with CD8+ cells for 5 days. The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. In the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)). (C) IL-10, <t>TNFα,</t> <t>IL-12p70</t> and IL1-β production of DC and tolDC, after 5 days of differentiation and 24 h of LPS (10 ng/μL) and IFNg (20 ng/μL) stimuli. P-values of paired t-tests are shown. (D) Box-plots of CD80, CD83, CD86 and HLA-DR surface expression (Median Fluorescence Intensity) in DCs and tolDCs in steady-state or stimulated with LPS (10 ng/μL) and IFNg (20 ng/μL) (ns p > 0.05, ** p < 0.01, *** p ≤ 0.001). (E) Gene expression heatmap of differentially expressed genes comparing tolDCs with DCs and also displaying the gene expression values of the precursor cell type (MO) (logFC > 0.5, FDR < 0.05). Scaled fluorescence values of expression arrays are shown, ranging from -2 (lower gene expression, green) to +2 (higher gene expression, orange). (F) Gene ontology (GO) over-representation of GO Biological Process categories. Fold change of tolDC induced genes over background and -log10(FDR) of Fisher’s exact tests are shown. (G) Discriminant regulon expression analysis (DoRothEA) of tolDC compared with DC. Only transcription factors with FDR < 0.05 are shown. NES and logFC of transcription factor expression are depicted. (H) T-distributed stochastic neighbor embedding (t-SNE) plot of the aggregated and batch-corrected gene expression data from our study (MO, DC and tolDC) and two additional public datasets (GSE40484 (moMAC, moDC, cDC2, CM (Classical MOs) and NCM (Non-Classical MOs) and GSE99056 (M-MAC (M2 Macrophages) and GM-MAC (M1 Macrophages)). The 4 different groups obtained using k-means clustering are represented with grey ellipses of multivariate t-distributions.
Foxp3 Efluor 450 Ebioscience, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/foxp3-efluor 450 ebioscience/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
foxp3-efluor 450 ebioscience - by Bioz Stars, 2026-02
90/100 stars
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enzyme-linked immunosorbent assay (elisa) perforin  (Mabtech Inc)
90
Mabtech Inc enzyme-linked immunosorbent assay (elisa) perforin
(A) Schematic representation of the experimental approach, comparing dendritic cell (DC) with tolerogenic dendritic cell (tolDC) differentiation. (B) DC and tolDC were cocultured with CD8+ cells for 5 days. The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. In the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)). (C) IL-10, <t>TNFα,</t> <t>IL-12p70</t> and IL1-β production of DC and tolDC, after 5 days of differentiation and 24 h of LPS (10 ng/μL) and IFNg (20 ng/μL) stimuli. P-values of paired t-tests are shown. (D) Box-plots of CD80, CD83, CD86 and HLA-DR surface expression (Median Fluorescence Intensity) in DCs and tolDCs in steady-state or stimulated with LPS (10 ng/μL) and IFNg (20 ng/μL) (ns p > 0.05, ** p < 0.01, *** p ≤ 0.001). (E) Gene expression heatmap of differentially expressed genes comparing tolDCs with DCs and also displaying the gene expression values of the precursor cell type (MO) (logFC > 0.5, FDR < 0.05). Scaled fluorescence values of expression arrays are shown, ranging from -2 (lower gene expression, green) to +2 (higher gene expression, orange). (F) Gene ontology (GO) over-representation of GO Biological Process categories. Fold change of tolDC induced genes over background and -log10(FDR) of Fisher’s exact tests are shown. (G) Discriminant regulon expression analysis (DoRothEA) of tolDC compared with DC. Only transcription factors with FDR < 0.05 are shown. NES and logFC of transcription factor expression are depicted. (H) T-distributed stochastic neighbor embedding (t-SNE) plot of the aggregated and batch-corrected gene expression data from our study (MO, DC and tolDC) and two additional public datasets (GSE40484 (moMAC, moDC, cDC2, CM (Classical MOs) and NCM (Non-Classical MOs) and GSE99056 (M-MAC (M2 Macrophages) and GM-MAC (M1 Macrophages)). The 4 different groups obtained using k-means clustering are represented with grey ellipses of multivariate t-distributions.
Enzyme Linked Immunosorbent Assay (Elisa) Perforin, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enzyme-linked immunosorbent assay (elisa) perforin/product/Mabtech Inc
Average 90 stars, based on 1 article reviews
enzyme-linked immunosorbent assay (elisa) perforin - by Bioz Stars, 2026-02
90/100 stars
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fitc anti-tnf (mp6-xt22)  (Becton Dickinson)
90
Becton Dickinson fitc anti-tnf (mp6-xt22)
(A) Schematic representation of the experimental approach, comparing dendritic cell (DC) with tolerogenic dendritic cell (tolDC) differentiation. (B) DC and tolDC were cocultured with CD8+ cells for 5 days. The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. In the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)). (C) IL-10, <t>TNFα,</t> <t>IL-12p70</t> and IL1-β production of DC and tolDC, after 5 days of differentiation and 24 h of LPS (10 ng/μL) and IFNg (20 ng/μL) stimuli. P-values of paired t-tests are shown. (D) Box-plots of CD80, CD83, CD86 and HLA-DR surface expression (Median Fluorescence Intensity) in DCs and tolDCs in steady-state or stimulated with LPS (10 ng/μL) and IFNg (20 ng/μL) (ns p > 0.05, ** p < 0.01, *** p ≤ 0.001). (E) Gene expression heatmap of differentially expressed genes comparing tolDCs with DCs and also displaying the gene expression values of the precursor cell type (MO) (logFC > 0.5, FDR < 0.05). Scaled fluorescence values of expression arrays are shown, ranging from -2 (lower gene expression, green) to +2 (higher gene expression, orange). (F) Gene ontology (GO) over-representation of GO Biological Process categories. Fold change of tolDC induced genes over background and -log10(FDR) of Fisher’s exact tests are shown. (G) Discriminant regulon expression analysis (DoRothEA) of tolDC compared with DC. Only transcription factors with FDR < 0.05 are shown. NES and logFC of transcription factor expression are depicted. (H) T-distributed stochastic neighbor embedding (t-SNE) plot of the aggregated and batch-corrected gene expression data from our study (MO, DC and tolDC) and two additional public datasets (GSE40484 (moMAC, moDC, cDC2, CM (Classical MOs) and NCM (Non-Classical MOs) and GSE99056 (M-MAC (M2 Macrophages) and GM-MAC (M1 Macrophages)). The 4 different groups obtained using k-means clustering are represented with grey ellipses of multivariate t-distributions.
Fitc Anti Tnf (Mp6 Xt22), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc anti-tnf (mp6-xt22)/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
fitc anti-tnf (mp6-xt22) - by Bioz Stars, 2026-02
90/100 stars
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commercial elisa kits  (Thermo Fisher)
90
Thermo Fisher commercial elisa kits
(A) Schematic representation of the experimental approach, comparing dendritic cell (DC) with tolerogenic dendritic cell (tolDC) differentiation. (B) DC and tolDC were cocultured with CD8+ cells for 5 days. The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. In the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)). (C) IL-10, <t>TNFα,</t> <t>IL-12p70</t> and IL1-β production of DC and tolDC, after 5 days of differentiation and 24 h of LPS (10 ng/μL) and IFNg (20 ng/μL) stimuli. P-values of paired t-tests are shown. (D) Box-plots of CD80, CD83, CD86 and HLA-DR surface expression (Median Fluorescence Intensity) in DCs and tolDCs in steady-state or stimulated with LPS (10 ng/μL) and IFNg (20 ng/μL) (ns p > 0.05, ** p < 0.01, *** p ≤ 0.001). (E) Gene expression heatmap of differentially expressed genes comparing tolDCs with DCs and also displaying the gene expression values of the precursor cell type (MO) (logFC > 0.5, FDR < 0.05). Scaled fluorescence values of expression arrays are shown, ranging from -2 (lower gene expression, green) to +2 (higher gene expression, orange). (F) Gene ontology (GO) over-representation of GO Biological Process categories. Fold change of tolDC induced genes over background and -log10(FDR) of Fisher’s exact tests are shown. (G) Discriminant regulon expression analysis (DoRothEA) of tolDC compared with DC. Only transcription factors with FDR < 0.05 are shown. NES and logFC of transcription factor expression are depicted. (H) T-distributed stochastic neighbor embedding (t-SNE) plot of the aggregated and batch-corrected gene expression data from our study (MO, DC and tolDC) and two additional public datasets (GSE40484 (moMAC, moDC, cDC2, CM (Classical MOs) and NCM (Non-Classical MOs) and GSE99056 (M-MAC (M2 Macrophages) and GM-MAC (M1 Macrophages)). The 4 different groups obtained using k-means clustering are represented with grey ellipses of multivariate t-distributions.
Commercial Elisa Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/commercial elisa kits/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
commercial elisa kits - by Bioz Stars, 2026-02
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tnf-α kit  (R&D Systems)
90
R&D Systems tnf-α kit
(A) Schematic representation of the experimental approach, comparing dendritic cell (DC) with tolerogenic dendritic cell (tolDC) differentiation. (B) DC and tolDC were cocultured with CD8+ cells for 5 days. The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. In the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)). (C) IL-10, <t>TNFα,</t> <t>IL-12p70</t> and IL1-β production of DC and tolDC, after 5 days of differentiation and 24 h of LPS (10 ng/μL) and IFNg (20 ng/μL) stimuli. P-values of paired t-tests are shown. (D) Box-plots of CD80, CD83, CD86 and HLA-DR surface expression (Median Fluorescence Intensity) in DCs and tolDCs in steady-state or stimulated with LPS (10 ng/μL) and IFNg (20 ng/μL) (ns p > 0.05, ** p < 0.01, *** p ≤ 0.001). (E) Gene expression heatmap of differentially expressed genes comparing tolDCs with DCs and also displaying the gene expression values of the precursor cell type (MO) (logFC > 0.5, FDR < 0.05). Scaled fluorescence values of expression arrays are shown, ranging from -2 (lower gene expression, green) to +2 (higher gene expression, orange). (F) Gene ontology (GO) over-representation of GO Biological Process categories. Fold change of tolDC induced genes over background and -log10(FDR) of Fisher’s exact tests are shown. (G) Discriminant regulon expression analysis (DoRothEA) of tolDC compared with DC. Only transcription factors with FDR < 0.05 are shown. NES and logFC of transcription factor expression are depicted. (H) T-distributed stochastic neighbor embedding (t-SNE) plot of the aggregated and batch-corrected gene expression data from our study (MO, DC and tolDC) and two additional public datasets (GSE40484 (moMAC, moDC, cDC2, CM (Classical MOs) and NCM (Non-Classical MOs) and GSE99056 (M-MAC (M2 Macrophages) and GM-MAC (M1 Macrophages)). The 4 different groups obtained using k-means clustering are represented with grey ellipses of multivariate t-distributions.
Tnf α Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tnf-α kit/product/R&D Systems
Average 90 stars, based on 1 article reviews
tnf-α kit - by Bioz Stars, 2026-02
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recombinant mouse tnf α  (Revvity)
95
Revvity recombinant mouse tnf α
(A) Schematic representation of the experimental approach, comparing dendritic cell (DC) with tolerogenic dendritic cell (tolDC) differentiation. (B) DC and tolDC were cocultured with CD8+ cells for 5 days. The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. In the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)). (C) IL-10, <t>TNFα,</t> <t>IL-12p70</t> and IL1-β production of DC and tolDC, after 5 days of differentiation and 24 h of LPS (10 ng/μL) and IFNg (20 ng/μL) stimuli. P-values of paired t-tests are shown. (D) Box-plots of CD80, CD83, CD86 and HLA-DR surface expression (Median Fluorescence Intensity) in DCs and tolDCs in steady-state or stimulated with LPS (10 ng/μL) and IFNg (20 ng/μL) (ns p > 0.05, ** p < 0.01, *** p ≤ 0.001). (E) Gene expression heatmap of differentially expressed genes comparing tolDCs with DCs and also displaying the gene expression values of the precursor cell type (MO) (logFC > 0.5, FDR < 0.05). Scaled fluorescence values of expression arrays are shown, ranging from -2 (lower gene expression, green) to +2 (higher gene expression, orange). (F) Gene ontology (GO) over-representation of GO Biological Process categories. Fold change of tolDC induced genes over background and -log10(FDR) of Fisher’s exact tests are shown. (G) Discriminant regulon expression analysis (DoRothEA) of tolDC compared with DC. Only transcription factors with FDR < 0.05 are shown. NES and logFC of transcription factor expression are depicted. (H) T-distributed stochastic neighbor embedding (t-SNE) plot of the aggregated and batch-corrected gene expression data from our study (MO, DC and tolDC) and two additional public datasets (GSE40484 (moMAC, moDC, cDC2, CM (Classical MOs) and NCM (Non-Classical MOs) and GSE99056 (M-MAC (M2 Macrophages) and GM-MAC (M1 Macrophages)). The 4 different groups obtained using k-means clustering are represented with grey ellipses of multivariate t-distributions.
Recombinant Mouse Tnf α, supplied by Revvity, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant mouse tnf α/product/Revvity
Average 95 stars, based on 1 article reviews
recombinant mouse tnf α - by Bioz Stars, 2026-02
95/100 stars
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pro inflammatory cytokines tnf α  (Revvity)
99
Revvity pro inflammatory cytokines tnf α
(A) Schematic representation of the experimental approach, comparing dendritic cell (DC) with tolerogenic dendritic cell (tolDC) differentiation. (B) DC and tolDC were cocultured with CD8+ cells for 5 days. The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. In the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)). (C) IL-10, <t>TNFα,</t> <t>IL-12p70</t> and IL1-β production of DC and tolDC, after 5 days of differentiation and 24 h of LPS (10 ng/μL) and IFNg (20 ng/μL) stimuli. P-values of paired t-tests are shown. (D) Box-plots of CD80, CD83, CD86 and HLA-DR surface expression (Median Fluorescence Intensity) in DCs and tolDCs in steady-state or stimulated with LPS (10 ng/μL) and IFNg (20 ng/μL) (ns p > 0.05, ** p < 0.01, *** p ≤ 0.001). (E) Gene expression heatmap of differentially expressed genes comparing tolDCs with DCs and also displaying the gene expression values of the precursor cell type (MO) (logFC > 0.5, FDR < 0.05). Scaled fluorescence values of expression arrays are shown, ranging from -2 (lower gene expression, green) to +2 (higher gene expression, orange). (F) Gene ontology (GO) over-representation of GO Biological Process categories. Fold change of tolDC induced genes over background and -log10(FDR) of Fisher’s exact tests are shown. (G) Discriminant regulon expression analysis (DoRothEA) of tolDC compared with DC. Only transcription factors with FDR < 0.05 are shown. NES and logFC of transcription factor expression are depicted. (H) T-distributed stochastic neighbor embedding (t-SNE) plot of the aggregated and batch-corrected gene expression data from our study (MO, DC and tolDC) and two additional public datasets (GSE40484 (moMAC, moDC, cDC2, CM (Classical MOs) and NCM (Non-Classical MOs) and GSE99056 (M-MAC (M2 Macrophages) and GM-MAC (M1 Macrophages)). The 4 different groups obtained using k-means clustering are represented with grey ellipses of multivariate t-distributions.
Pro Inflammatory Cytokines Tnf α, supplied by Revvity, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pro inflammatory cytokines tnf α/product/Revvity
Average 99 stars, based on 1 article reviews
pro inflammatory cytokines tnf α - by Bioz Stars, 2026-02
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mouse tnf α  (Revvity)
92
Revvity mouse tnf α
(A) Schematic representation of the experimental approach, comparing dendritic cell (DC) with tolerogenic dendritic cell (tolDC) differentiation. (B) DC and tolDC were cocultured with CD8+ cells for 5 days. The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. In the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)). (C) IL-10, <t>TNFα,</t> <t>IL-12p70</t> and IL1-β production of DC and tolDC, after 5 days of differentiation and 24 h of LPS (10 ng/μL) and IFNg (20 ng/μL) stimuli. P-values of paired t-tests are shown. (D) Box-plots of CD80, CD83, CD86 and HLA-DR surface expression (Median Fluorescence Intensity) in DCs and tolDCs in steady-state or stimulated with LPS (10 ng/μL) and IFNg (20 ng/μL) (ns p > 0.05, ** p < 0.01, *** p ≤ 0.001). (E) Gene expression heatmap of differentially expressed genes comparing tolDCs with DCs and also displaying the gene expression values of the precursor cell type (MO) (logFC > 0.5, FDR < 0.05). Scaled fluorescence values of expression arrays are shown, ranging from -2 (lower gene expression, green) to +2 (higher gene expression, orange). (F) Gene ontology (GO) over-representation of GO Biological Process categories. Fold change of tolDC induced genes over background and -log10(FDR) of Fisher’s exact tests are shown. (G) Discriminant regulon expression analysis (DoRothEA) of tolDC compared with DC. Only transcription factors with FDR < 0.05 are shown. NES and logFC of transcription factor expression are depicted. (H) T-distributed stochastic neighbor embedding (t-SNE) plot of the aggregated and batch-corrected gene expression data from our study (MO, DC and tolDC) and two additional public datasets (GSE40484 (moMAC, moDC, cDC2, CM (Classical MOs) and NCM (Non-Classical MOs) and GSE99056 (M-MAC (M2 Macrophages) and GM-MAC (M1 Macrophages)). The 4 different groups obtained using k-means clustering are represented with grey ellipses of multivariate t-distributions.
Mouse Tnf α, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse tnf α/product/Revvity
Average 92 stars, based on 1 article reviews
mouse tnf α - by Bioz Stars, 2026-02
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tnf-α  (Dakewe Biotech Co)
90
Dakewe Biotech Co tnf-α
(A) Schematic representation of the experimental approach, comparing dendritic cell (DC) with tolerogenic dendritic cell (tolDC) differentiation. (B) DC and tolDC were cocultured with CD8+ cells for 5 days. The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. In the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)). (C) IL-10, <t>TNFα,</t> <t>IL-12p70</t> and IL1-β production of DC and tolDC, after 5 days of differentiation and 24 h of LPS (10 ng/μL) and IFNg (20 ng/μL) stimuli. P-values of paired t-tests are shown. (D) Box-plots of CD80, CD83, CD86 and HLA-DR surface expression (Median Fluorescence Intensity) in DCs and tolDCs in steady-state or stimulated with LPS (10 ng/μL) and IFNg (20 ng/μL) (ns p > 0.05, ** p < 0.01, *** p ≤ 0.001). (E) Gene expression heatmap of differentially expressed genes comparing tolDCs with DCs and also displaying the gene expression values of the precursor cell type (MO) (logFC > 0.5, FDR < 0.05). Scaled fluorescence values of expression arrays are shown, ranging from -2 (lower gene expression, green) to +2 (higher gene expression, orange). (F) Gene ontology (GO) over-representation of GO Biological Process categories. Fold change of tolDC induced genes over background and -log10(FDR) of Fisher’s exact tests are shown. (G) Discriminant regulon expression analysis (DoRothEA) of tolDC compared with DC. Only transcription factors with FDR < 0.05 are shown. NES and logFC of transcription factor expression are depicted. (H) T-distributed stochastic neighbor embedding (t-SNE) plot of the aggregated and batch-corrected gene expression data from our study (MO, DC and tolDC) and two additional public datasets (GSE40484 (moMAC, moDC, cDC2, CM (Classical MOs) and NCM (Non-Classical MOs) and GSE99056 (M-MAC (M2 Macrophages) and GM-MAC (M1 Macrophages)). The 4 different groups obtained using k-means clustering are represented with grey ellipses of multivariate t-distributions.
Tnf α, supplied by Dakewe Biotech Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tnf-α/product/Dakewe Biotech Co
Average 90 stars, based on 1 article reviews
tnf-α - by Bioz Stars, 2026-02
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(A) Schematic representation of the experimental approach, comparing dendritic cell (DC) with tolerogenic dendritic cell (tolDC) differentiation. (B) DC and tolDC were cocultured with CD8+ cells for 5 days. The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. In the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)). (C) IL-10, TNFα, IL-12p70 and IL1-β production of DC and tolDC, after 5 days of differentiation and 24 h of LPS (10 ng/μL) and IFNg (20 ng/μL) stimuli. P-values of paired t-tests are shown. (D) Box-plots of CD80, CD83, CD86 and HLA-DR surface expression (Median Fluorescence Intensity) in DCs and tolDCs in steady-state or stimulated with LPS (10 ng/μL) and IFNg (20 ng/μL) (ns p > 0.05, ** p < 0.01, *** p ≤ 0.001). (E) Gene expression heatmap of differentially expressed genes comparing tolDCs with DCs and also displaying the gene expression values of the precursor cell type (MO) (logFC > 0.5, FDR < 0.05). Scaled fluorescence values of expression arrays are shown, ranging from -2 (lower gene expression, green) to +2 (higher gene expression, orange). (F) Gene ontology (GO) over-representation of GO Biological Process categories. Fold change of tolDC induced genes over background and -log10(FDR) of Fisher’s exact tests are shown. (G) Discriminant regulon expression analysis (DoRothEA) of tolDC compared with DC. Only transcription factors with FDR < 0.05 are shown. NES and logFC of transcription factor expression are depicted. (H) T-distributed stochastic neighbor embedding (t-SNE) plot of the aggregated and batch-corrected gene expression data from our study (MO, DC and tolDC) and two additional public datasets (GSE40484 (moMAC, moDC, cDC2, CM (Classical MOs) and NCM (Non-Classical MOs) and GSE99056 (M-MAC (M2 Macrophages) and GM-MAC (M1 Macrophages)). The 4 different groups obtained using k-means clustering are represented with grey ellipses of multivariate t-distributions.

Journal: bioRxiv

Article Title: MAFB surrogates the glucocorticoid receptor ability to induce tolerogenesis in dendritic cells

doi: 10.1101/2021.07.27.453975

Figure Lengend Snippet: (A) Schematic representation of the experimental approach, comparing dendritic cell (DC) with tolerogenic dendritic cell (tolDC) differentiation. (B) DC and tolDC were cocultured with CD8+ cells for 5 days. The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. In the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)). (C) IL-10, TNFα, IL-12p70 and IL1-β production of DC and tolDC, after 5 days of differentiation and 24 h of LPS (10 ng/μL) and IFNg (20 ng/μL) stimuli. P-values of paired t-tests are shown. (D) Box-plots of CD80, CD83, CD86 and HLA-DR surface expression (Median Fluorescence Intensity) in DCs and tolDCs in steady-state or stimulated with LPS (10 ng/μL) and IFNg (20 ng/μL) (ns p > 0.05, ** p < 0.01, *** p ≤ 0.001). (E) Gene expression heatmap of differentially expressed genes comparing tolDCs with DCs and also displaying the gene expression values of the precursor cell type (MO) (logFC > 0.5, FDR < 0.05). Scaled fluorescence values of expression arrays are shown, ranging from -2 (lower gene expression, green) to +2 (higher gene expression, orange). (F) Gene ontology (GO) over-representation of GO Biological Process categories. Fold change of tolDC induced genes over background and -log10(FDR) of Fisher’s exact tests are shown. (G) Discriminant regulon expression analysis (DoRothEA) of tolDC compared with DC. Only transcription factors with FDR < 0.05 are shown. NES and logFC of transcription factor expression are depicted. (H) T-distributed stochastic neighbor embedding (t-SNE) plot of the aggregated and batch-corrected gene expression data from our study (MO, DC and tolDC) and two additional public datasets (GSE40484 (moMAC, moDC, cDC2, CM (Classical MOs) and NCM (Non-Classical MOs) and GSE99056 (M-MAC (M2 Macrophages) and GM-MAC (M1 Macrophages)). The 4 different groups obtained using k-means clustering are represented with grey ellipses of multivariate t-distributions.

Article Snippet: Enzyme-linked immunosorbent assays (ELISA) were performed, following the manufacturer’s instructions: Human IL-10, Human IL-12p70, and Human TNFα from BioLegend, and Human IL-1β from ThermoFisher.

Techniques: Expressing, Fluorescence, Gene Expression

(A) Volcano plot comparing tolDCs treated with control siRNA (siCTL) and MAFB siRNA (siMAFB). Dashed lines indicate significance thresholds (FDR < 0.05, absolute logFC > 0.5). tolDC-induced and tolDC-repressed genes are shown in blue and orange, respectively. (B) Gene set enrichment analysis (GSEA) of tolDCs (siCTL) vs. tolDCs (siMAFB), using tolDC-induced and tolDC-repressed gene sets. The running enrichment score is represented and the normalized enrichment score (NES) is shown above (FDR < 0.01). (C) DNA methylation heatmap of previously obtained differentially methylated CpGs (C1-CpGs and C2-CpGs) in tolDCs (siCTL) and tolDCs (siMAFB). Scaled β-values are shown (lower DNA methylation levels in blue and higher methylation levels in red). On the right side, violin plots of Cluster 1 (C1) and Cluster 2 (C2) depict β-values (ns p > 0.05, *** p ≤ 0.001). (D) Methylated CpG set enrichment analysis (mCSEA) of tolDCs (siCTL) vs. tolDCs (siMAFB), using MAFB-only CpGs, GR/MAFB CpGs and GR-only CpGs as CpG-sets (depending on the overlap of CpGs with GR or MAFB peaks). The running enrichment score is represented and the normalized enrichment score (NES) and FDR are shown above. (E) Box-plots of median fluorescence intensity (MFI) of CD14, CD16, CD163 and CD1a flow cytometry data from DCs (siCTL), tolDCs (siCTL) and tolDCs (siMAFB) (n = 7) (ns p > 0.05, * p < 0.05, ** p ≤ 0.01). (F) Box-plots of supernatant concentration from DCs (siCTL), tolDCs (siCTL) and tolDCs (siMAFB) (n = 7) of IL-10 in steady-state and stimulated conditions (LPS 10 ng/μL and IFNγ 20 ng/μL) and IL-12p70 and TNFα under stimulated conditions (pg/mL). TNFα and IL-12p70 in steady state were not detected. (ns p > 0.05, * p < 0.05, ** p ≤ 0.01) (G) DC (siCTL), tolDC (siCTL) and tolDC (siMAFB) were cocultured with CD8+ cells for 5 days (n = 4). The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. On the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)) (** p ≤ 0.01, *** p ≤ 0.001).

Journal: bioRxiv

Article Title: MAFB surrogates the glucocorticoid receptor ability to induce tolerogenesis in dendritic cells

doi: 10.1101/2021.07.27.453975

Figure Lengend Snippet: (A) Volcano plot comparing tolDCs treated with control siRNA (siCTL) and MAFB siRNA (siMAFB). Dashed lines indicate significance thresholds (FDR < 0.05, absolute logFC > 0.5). tolDC-induced and tolDC-repressed genes are shown in blue and orange, respectively. (B) Gene set enrichment analysis (GSEA) of tolDCs (siCTL) vs. tolDCs (siMAFB), using tolDC-induced and tolDC-repressed gene sets. The running enrichment score is represented and the normalized enrichment score (NES) is shown above (FDR < 0.01). (C) DNA methylation heatmap of previously obtained differentially methylated CpGs (C1-CpGs and C2-CpGs) in tolDCs (siCTL) and tolDCs (siMAFB). Scaled β-values are shown (lower DNA methylation levels in blue and higher methylation levels in red). On the right side, violin plots of Cluster 1 (C1) and Cluster 2 (C2) depict β-values (ns p > 0.05, *** p ≤ 0.001). (D) Methylated CpG set enrichment analysis (mCSEA) of tolDCs (siCTL) vs. tolDCs (siMAFB), using MAFB-only CpGs, GR/MAFB CpGs and GR-only CpGs as CpG-sets (depending on the overlap of CpGs with GR or MAFB peaks). The running enrichment score is represented and the normalized enrichment score (NES) and FDR are shown above. (E) Box-plots of median fluorescence intensity (MFI) of CD14, CD16, CD163 and CD1a flow cytometry data from DCs (siCTL), tolDCs (siCTL) and tolDCs (siMAFB) (n = 7) (ns p > 0.05, * p < 0.05, ** p ≤ 0.01). (F) Box-plots of supernatant concentration from DCs (siCTL), tolDCs (siCTL) and tolDCs (siMAFB) (n = 7) of IL-10 in steady-state and stimulated conditions (LPS 10 ng/μL and IFNγ 20 ng/μL) and IL-12p70 and TNFα under stimulated conditions (pg/mL). TNFα and IL-12p70 in steady state were not detected. (ns p > 0.05, * p < 0.05, ** p ≤ 0.01) (G) DC (siCTL), tolDC (siCTL) and tolDC (siMAFB) were cocultured with CD8+ cells for 5 days (n = 4). The final CFSE signal of CD8+ cells is shown (left panel). CD8+ with only CD3/CD28 T-activator beads (C+) or alone (C-) are also shown. On the right panel, the average proliferation of the quadruplicate is shown (mean ± standard error of the mean (SEM)) (** p ≤ 0.01, *** p ≤ 0.001).

Article Snippet: Enzyme-linked immunosorbent assays (ELISA) were performed, following the manufacturer’s instructions: Human IL-10, Human IL-12p70, and Human TNFα from BioLegend, and Human IL-1β from ThermoFisher.

Techniques: Control, DNA Methylation Assay, Methylation, Fluorescence, Flow Cytometry, Concentration Assay