Journal: Frontiers in Microbiology
Article Title: B-Cell Lymphoma 6 (BCL6) Is a Host Restriction Factor That Can Suppress HBV Gene Expression and Modulate Immune Responses
Figure Lengend Snippet: TNF-α induces Bcl6 gene expression in the hepatocytes and Bcl6 overexpression upregulates the TNF-α levels in vivo . (A) The RNA levels of Tnf- α, Il-6, Il-12, Cxcl9, and Cxcl10 were examined by RT-qPCR in B6.2- and B6.2S-injected mouse liver isolated during the HBV clearance stage. (B) AML12 cells were treated with or without TNF-α (100 ng/ml) for the indicated time periods and Bcl6 RNA was analyzed by RT-qPCR. The induction fold means the ratio of the Bcl6 RNA levels in the presence of TNF-α over those without TNF-α. (C) AML12 and Huh-7 cells were transfected with 1 μg of vector or the Bcl6 -expressing plasmid. Two days later, the TNF-α RNA levels were analyzed by RT-qPCR analysis. (D) The liver RNA from the B6.2 or the (B6.2 + Bcl6 ) mice at 2 w.p.i. were analyzed for Tnf- α expression by RT-qPCR. In (A–D) , * P
Article Snippet: The cytokines used for stimulating Bcl6 expression or chemokine induction included TNF-α (315-01A, PeProtech, USA) and IFN-γ (485MI, R & D, USA).
Techniques: Expressing, Over Expression, In Vivo, Quantitative RT-PCR, Injection, Isolation, Transfection, Plasmid Preparation, Mouse Assay