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  • 99
    Bio-Rad 96 well thin wall pcr plate
    96 Well Thin Wall Pcr Plate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Harvard Bioscience thin walled borosilicate glass
    Thin Walled Borosilicate Glass, supplied by Harvard Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 158 article reviews
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    90
    Harvard Bioscience thin walled borosilicate glass capillaries
    Thin Walled Borosilicate Glass Capillaries, supplied by Harvard Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Avantor thin walled glass capillaries
    Thin Walled Glass Capillaries, supplied by Avantor, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher geneamp thin walled reaction tube with domed cap autoclaved
    The ABI 0.5 mL <t>GeneAmp</t> tube containing 10 uL Lysis Buffer.
    Geneamp Thin Walled Reaction Tube With Domed Cap Autoclaved, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 24 article reviews
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    84
    World Precision Instruments thin walled glass capillary
    The ABI 0.5 mL <t>GeneAmp</t> tube containing 10 uL Lysis Buffer.
    Thin Walled Glass Capillary, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 84/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Sutter Instrument thin walled glass pipettes
    The ABI 0.5 mL <t>GeneAmp</t> tube containing 10 uL Lysis Buffer.
    Thin Walled Glass Pipettes, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 84/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Agilent technologies thin walled pcr plates
    Characterizations of NAb 2G8. ( A ) Neutralization assay of CVA10 NAb 2G8. The IC 50 value is 0.2 μg/ml. ( B ) Binding efficacy of NAb 2G8 evaluated with the indirect ELISA. NAb 2G8 exhibits high binding affinities with the CVA10 particles [median effective concentration (EC 50 ) values from 83.1 to 251.1 ng/ml]. The IC 50 and EC 50 values were calculated with nonlinear regression fitting curves. The optical density (OD) value was read at A 450/620 . ( C ) The competitive ELISA of NAb 2G8. The binding of NAb 2G8 to CVA10 particles was significantly blocked by sera from mice immunized with CVA10 and CVA10-positive human sera. The percentages of blocking are expressed as means ± SD. ( D ) In vivo preventive and therapeutic efficacy of NAb 2G8. The infected mice were treated with NAb 2G8 12 hours before (blue line) or 24 hours after (red line) infection intraperitoneally with CVA10 and monitored daily after inoculation. Both experimental groups showed 100% survival rates, but 0% for two control groups. ( E ) Neutralization assay of NAb 2G8 at pre-attachment (blue line) or post-attachment (red line). NAb 2G8 exhibits much higher neutralizing capacity at pre-attachment (IC 50 = 2.1 μg/ml) than at post-attachment (IC 50 = 22.7 μg/ml). ( F ) Amount of cell-bound CVA10 viruses detected by <t>RT-PCR.</t> Indicated with the relative CVA10 genome level, the amount of cell-bound viruses gradually decreased as a function of increasing NAb 2G8 concentrations in both cases of pre- and post-attachment. Values are expressed as means ± SD. Experiments were repeated in triplicate. <t>PBS,</t> phosphate-buffered saline.
    Thin Walled Pcr Plates, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 84/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Bio-Rad hard shell thin wall 384 well pcr plates
    Characterizations of NAb 2G8. ( A ) Neutralization assay of CVA10 NAb 2G8. The IC 50 value is 0.2 μg/ml. ( B ) Binding efficacy of NAb 2G8 evaluated with the indirect ELISA. NAb 2G8 exhibits high binding affinities with the CVA10 particles [median effective concentration (EC 50 ) values from 83.1 to 251.1 ng/ml]. The IC 50 and EC 50 values were calculated with nonlinear regression fitting curves. The optical density (OD) value was read at A 450/620 . ( C ) The competitive ELISA of NAb 2G8. The binding of NAb 2G8 to CVA10 particles was significantly blocked by sera from mice immunized with CVA10 and CVA10-positive human sera. The percentages of blocking are expressed as means ± SD. ( D ) In vivo preventive and therapeutic efficacy of NAb 2G8. The infected mice were treated with NAb 2G8 12 hours before (blue line) or 24 hours after (red line) infection intraperitoneally with CVA10 and monitored daily after inoculation. Both experimental groups showed 100% survival rates, but 0% for two control groups. ( E ) Neutralization assay of NAb 2G8 at pre-attachment (blue line) or post-attachment (red line). NAb 2G8 exhibits much higher neutralizing capacity at pre-attachment (IC 50 = 2.1 μg/ml) than at post-attachment (IC 50 = 22.7 μg/ml). ( F ) Amount of cell-bound CVA10 viruses detected by <t>RT-PCR.</t> Indicated with the relative CVA10 genome level, the amount of cell-bound viruses gradually decreased as a function of increasing NAb 2G8 concentrations in both cases of pre- and post-attachment. Values are expressed as means ± SD. Experiments were repeated in triplicate. <t>PBS,</t> phosphate-buffered saline.
    Hard Shell Thin Wall 384 Well Pcr Plates, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    World Precision Instruments thin walled borosilicate glass pipettes
    Characterizations of NAb 2G8. ( A ) Neutralization assay of CVA10 NAb 2G8. The IC 50 value is 0.2 μg/ml. ( B ) Binding efficacy of NAb 2G8 evaluated with the indirect ELISA. NAb 2G8 exhibits high binding affinities with the CVA10 particles [median effective concentration (EC 50 ) values from 83.1 to 251.1 ng/ml]. The IC 50 and EC 50 values were calculated with nonlinear regression fitting curves. The optical density (OD) value was read at A 450/620 . ( C ) The competitive ELISA of NAb 2G8. The binding of NAb 2G8 to CVA10 particles was significantly blocked by sera from mice immunized with CVA10 and CVA10-positive human sera. The percentages of blocking are expressed as means ± SD. ( D ) In vivo preventive and therapeutic efficacy of NAb 2G8. The infected mice were treated with NAb 2G8 12 hours before (blue line) or 24 hours after (red line) infection intraperitoneally with CVA10 and monitored daily after inoculation. Both experimental groups showed 100% survival rates, but 0% for two control groups. ( E ) Neutralization assay of NAb 2G8 at pre-attachment (blue line) or post-attachment (red line). NAb 2G8 exhibits much higher neutralizing capacity at pre-attachment (IC 50 = 2.1 μg/ml) than at post-attachment (IC 50 = 22.7 μg/ml). ( F ) Amount of cell-bound CVA10 viruses detected by <t>RT-PCR.</t> Indicated with the relative CVA10 genome level, the amount of cell-bound viruses gradually decreased as a function of increasing NAb 2G8 concentrations in both cases of pre- and post-attachment. Values are expressed as means ± SD. Experiments were repeated in triplicate. <t>PBS,</t> phosphate-buffered saline.
    Thin Walled Borosilicate Glass Pipettes, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 81/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Sorenson BioScience thin walled pcr tubes
    Photos of the <t>PCR</t> tubes illuminated with blue LED. Fluorescent photos of SYBR-Green based PCR reagents tubes after amplification steps in a commercial cycler and the TTC. The intensity of NTC tube is much lower than the tubes containing template <t>DNA.</t>
    Thin Walled Pcr Tubes, supplied by Sorenson BioScience, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Paradigm Optics Inc thin walled polycarbonate microtubes
    In vitro: ULM images (A-C) and super harmonic images (D-F) of <t>microtubes</t> with inner diameter of 150 µm (A, D), 75 µm (B, E) and 50 µm (C, F). Bar = 200 µm.
    Thin Walled Polycarbonate Microtubes, supplied by Paradigm Optics Inc, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher thin wall pcr plates
    The miRNA expression level of has-miR-214* (a), has-miR-105 (b), has-miR-548n (c), and has-miR-514b (d) detected by <t>qRT-PCR</t> in cancerous and normal tissues. Relative gene expression was calculated as 2 −ΔΔCT .
    Thin Wall Pcr Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The ABI 0.5 mL GeneAmp tube containing 10 uL Lysis Buffer.

    Journal: Biological Procedures Online

    Article Title: New quick method for isolating RNA from laser captured cells stained by immunofluorescent immunohistochemistry; RNA suitable for direct use in fluorogenic TaqMan one-step real-time RT-PCR

    doi: 10.1251/bpo107

    Figure Lengend Snippet: The ABI 0.5 mL GeneAmp tube containing 10 uL Lysis Buffer.

    Article Snippet: Immediately post capture, these tab/polymer stickers are peeled away from each cap with a nuclease-free instrument (e.g. forceps pre-treated by baking at 180°C for 6 hours and then sprayed with Ambion’s RNAse ZAP then rinsed with nuclease-free water) and each is placed into a separate 0.5 ml nuclease-free microfuge tube (Product #N8010611, GeneAmp Tube, ABI) already containing 10 μl of Lysis Buffer (made up of Invitrogen’s Resuspension Buffer, Lysis enhancer and RNAseOUT reagents mixed 10 to 1.1 to 1, e.g. 10:1.1:1, respectively).

    Techniques: Lysis

    Characterizations of NAb 2G8. ( A ) Neutralization assay of CVA10 NAb 2G8. The IC 50 value is 0.2 μg/ml. ( B ) Binding efficacy of NAb 2G8 evaluated with the indirect ELISA. NAb 2G8 exhibits high binding affinities with the CVA10 particles [median effective concentration (EC 50 ) values from 83.1 to 251.1 ng/ml]. The IC 50 and EC 50 values were calculated with nonlinear regression fitting curves. The optical density (OD) value was read at A 450/620 . ( C ) The competitive ELISA of NAb 2G8. The binding of NAb 2G8 to CVA10 particles was significantly blocked by sera from mice immunized with CVA10 and CVA10-positive human sera. The percentages of blocking are expressed as means ± SD. ( D ) In vivo preventive and therapeutic efficacy of NAb 2G8. The infected mice were treated with NAb 2G8 12 hours before (blue line) or 24 hours after (red line) infection intraperitoneally with CVA10 and monitored daily after inoculation. Both experimental groups showed 100% survival rates, but 0% for two control groups. ( E ) Neutralization assay of NAb 2G8 at pre-attachment (blue line) or post-attachment (red line). NAb 2G8 exhibits much higher neutralizing capacity at pre-attachment (IC 50 = 2.1 μg/ml) than at post-attachment (IC 50 = 22.7 μg/ml). ( F ) Amount of cell-bound CVA10 viruses detected by RT-PCR. Indicated with the relative CVA10 genome level, the amount of cell-bound viruses gradually decreased as a function of increasing NAb 2G8 concentrations in both cases of pre- and post-attachment. Values are expressed as means ± SD. Experiments were repeated in triplicate. PBS, phosphate-buffered saline.

    Journal: Science Advances

    Article Title: Discovery and structural characterization of a therapeutic antibody against coxsackievirus A10

    doi: 10.1126/sciadv.aat7459

    Figure Lengend Snippet: Characterizations of NAb 2G8. ( A ) Neutralization assay of CVA10 NAb 2G8. The IC 50 value is 0.2 μg/ml. ( B ) Binding efficacy of NAb 2G8 evaluated with the indirect ELISA. NAb 2G8 exhibits high binding affinities with the CVA10 particles [median effective concentration (EC 50 ) values from 83.1 to 251.1 ng/ml]. The IC 50 and EC 50 values were calculated with nonlinear regression fitting curves. The optical density (OD) value was read at A 450/620 . ( C ) The competitive ELISA of NAb 2G8. The binding of NAb 2G8 to CVA10 particles was significantly blocked by sera from mice immunized with CVA10 and CVA10-positive human sera. The percentages of blocking are expressed as means ± SD. ( D ) In vivo preventive and therapeutic efficacy of NAb 2G8. The infected mice were treated with NAb 2G8 12 hours before (blue line) or 24 hours after (red line) infection intraperitoneally with CVA10 and monitored daily after inoculation. Both experimental groups showed 100% survival rates, but 0% for two control groups. ( E ) Neutralization assay of NAb 2G8 at pre-attachment (blue line) or post-attachment (red line). NAb 2G8 exhibits much higher neutralizing capacity at pre-attachment (IC 50 = 2.1 μg/ml) than at post-attachment (IC 50 = 22.7 μg/ml). ( F ) Amount of cell-bound CVA10 viruses detected by RT-PCR. Indicated with the relative CVA10 genome level, the amount of cell-bound viruses gradually decreased as a function of increasing NAb 2G8 concentrations in both cases of pre- and post-attachment. Values are expressed as means ± SD. Experiments were repeated in triplicate. PBS, phosphate-buffered saline.

    Article Snippet: Each 50-μl reaction mixture, containing 1 μg of virus and 5 μM SYTO9 in PBS buffer, was set up in thin-walled PCR plates (Agilent).

    Techniques: Neutralization, Binding Assay, Indirect ELISA, Concentration Assay, Competitive ELISA, Mouse Assay, Blocking Assay, In Vivo, Infection, Reverse Transcription Polymerase Chain Reaction

    Photos of the PCR tubes illuminated with blue LED. Fluorescent photos of SYBR-Green based PCR reagents tubes after amplification steps in a commercial cycler and the TTC. The intensity of NTC tube is much lower than the tubes containing template DNA.

    Journal: PLoS ONE

    Article Title: A Rapid and Low-Cost PCR Thermal Cycler for Low Resource Settings

    doi: 10.1371/journal.pone.0131701

    Figure Lengend Snippet: Photos of the PCR tubes illuminated with blue LED. Fluorescent photos of SYBR-Green based PCR reagents tubes after amplification steps in a commercial cycler and the TTC. The intensity of NTC tube is much lower than the tubes containing template DNA.

    Article Snippet: By using extracted Staphylococcus aureus (ATCC 29213) template, PCR reactions targeting the nuc gene (281 bp) were performed using PrimeSTAR Max DNA polymerase master mix in thin-walled PCR tubes (Cat. No. 16950, Sorenson Bioscience Corp, Murray, UT) to amplify the extracted template [ ].

    Techniques: Polymerase Chain Reaction, SYBR Green Assay, Amplification

    Speed and sensitivity of PCR reactions demonstrated by TTC. PCR reactions to amplify 281 bp of the nuc gene from 2000, 200, 20, or 2 copies of S . aureus genomic DNA. (A) Lanes 1 to 4: PCR products from commercial thermal cycler. Lane 5: ladder. Lanes 6 to 9: PCR products from TTC with thin-walled plastic tubes, using a protocol of 2 min hot-start, followed by 40 cycles of 11 s and 17 s denaturation and annealing/extension. The 40-cycle reactions were completed in 22 min. (B) Lanes 1 to 4: PCR products from commercial thermal cycler. Lane 5: ladder. Lanes 6 to 9: PCR products from TTC with glass capillary tubes, using a protocol of 2 min hot-start, followed by 40 cycles of 7 s and 8 s denaturation and annealing/extension. The reactions were completed in 13 min and 3 s.

    Journal: PLoS ONE

    Article Title: A Rapid and Low-Cost PCR Thermal Cycler for Low Resource Settings

    doi: 10.1371/journal.pone.0131701

    Figure Lengend Snippet: Speed and sensitivity of PCR reactions demonstrated by TTC. PCR reactions to amplify 281 bp of the nuc gene from 2000, 200, 20, or 2 copies of S . aureus genomic DNA. (A) Lanes 1 to 4: PCR products from commercial thermal cycler. Lane 5: ladder. Lanes 6 to 9: PCR products from TTC with thin-walled plastic tubes, using a protocol of 2 min hot-start, followed by 40 cycles of 11 s and 17 s denaturation and annealing/extension. The 40-cycle reactions were completed in 22 min. (B) Lanes 1 to 4: PCR products from commercial thermal cycler. Lane 5: ladder. Lanes 6 to 9: PCR products from TTC with glass capillary tubes, using a protocol of 2 min hot-start, followed by 40 cycles of 7 s and 8 s denaturation and annealing/extension. The reactions were completed in 13 min and 3 s.

    Article Snippet: By using extracted Staphylococcus aureus (ATCC 29213) template, PCR reactions targeting the nuc gene (281 bp) were performed using PrimeSTAR Max DNA polymerase master mix in thin-walled PCR tubes (Cat. No. 16950, Sorenson Bioscience Corp, Murray, UT) to amplify the extracted template [ ].

    Techniques: Polymerase Chain Reaction

    In vitro: ULM images (A-C) and super harmonic images (D-F) of microtubes with inner diameter of 150 µm (A, D), 75 µm (B, E) and 50 µm (C, F). Bar = 200 µm.

    Journal: Theranostics

    Article Title: 3-D Ultrasound Localization Microscopy for Identifying Microvascular Morphology Features of Tumor Angiogenesis at a Resolution Beyond the Diffraction Limit of Conventional Ultrasound

    doi: 10.7150/thno.16899

    Figure Lengend Snippet: In vitro: ULM images (A-C) and super harmonic images (D-F) of microtubes with inner diameter of 150 µm (A, D), 75 µm (B, E) and 50 µm (C, F). Bar = 200 µm.

    Article Snippet: In order to validate improvements in resolution via the ULM technique, in vitro studies were first conducted using simulated vessels from thin-walled polycarbonate microtubes (Paradigm Optics Inc., WA, USA) with inner diameters of 150 µm, 75 µm and 50 µm.

    Techniques: In Vitro

    The miRNA expression level of has-miR-214* (a), has-miR-105 (b), has-miR-548n (c), and has-miR-514b (d) detected by qRT-PCR in cancerous and normal tissues. Relative gene expression was calculated as 2 −ΔΔCT .

    Journal: Gastroenterology Research and Practice

    Article Title: Identification of Aberrantly Expressed miRNAs in Gastric Cancer

    doi: 10.1155/2014/473817

    Figure Lengend Snippet: The miRNA expression level of has-miR-214* (a), has-miR-105 (b), has-miR-548n (c), and has-miR-514b (d) detected by qRT-PCR in cancerous and normal tissues. Relative gene expression was calculated as 2 −ΔΔCT .

    Article Snippet: In brief, one microgram of extracted RNA was reverse-transcribed per the manufacturer's instructions. qRT-PCR was carried out in thin-wall PCR plates (Applied Biosystems, Foster City, CA, USA).

    Techniques: Expressing, Quantitative RT-PCR

    Example of a 3% agarose gel showing SPF10 PCR products Five μl PCR product was loaded on a 3% agarose gel and visualized using Ethidium Bromide. Lane 1 contained DNA from an HPV negative sample, lane 2 and 3 were previously typed as HPV16 and HPV18 positive respectively. NTC indicates the no-template negative control (double distilled H2 O). 1E-4 ng HPV18 genome cloned in pBR322 was used as a positive control.

    Journal:

    Article Title: Detection and genotyping of Human Papillomaviruses from archival formalin fixed tissue samples

    doi: 10.1002/cpmc.16

    Figure Lengend Snippet: Example of a 3% agarose gel showing SPF10 PCR products Five μl PCR product was loaded on a 3% agarose gel and visualized using Ethidium Bromide. Lane 1 contained DNA from an HPV negative sample, lane 2 and 3 were previously typed as HPV16 and HPV18 positive respectively. NTC indicates the no-template negative control (double distilled H2 O). 1E-4 ng HPV18 genome cloned in pBR322 was used as a positive control.

    Article Snippet: 11732668001) Agarose Gel purification kit (e.g. zymoclean Gel DNA recovery kit; Zymo. cat. no. D4007) 10X ThermoPol Buffer (NEB cat. no. B9004) 10 mM dATP Taq DNA Polymerase (NEB cat. no. M0267) 200 μl thin-walled PCR tubes (or other appropriate for thermal cycler) TOPO TA cloning kit (ThermoFisher cat. no. K450001) Topoisomerase I-activated pCR™2.1-TOPO® vector Salt solution (1.2 M NaCl and 0.06 M MgCl2 ) M13 forward and reverse primers One Shot Chemically Competent E. coli (TOP10; F– mcr A Δ( mrr - hsd RMS- mcr BC) Φ80 lac ZΔM15 Δ lac X74 rec A1 ara D139 Δ( ara leu ) 7697 gal U gal K rps L (StrR) end A1 nup G) S.O.C. medium (see recipe) LB plates containing 50 μg/ml Kanamycin or Carbenicillin Miniprep kit (e.g. Wizard Plus SV Miniprep; Promega, cat. no. A1270) Thermal cycler

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Negative Control, Clone Assay, Positive Control

    Flow chart of the different methods in the Protocol Following DNA extraction, the Protocol describes three complementary methods aimed at PCR amplifying fragments from the papillomavirus L1 open reading frame. Following PCR, the amplicons are sequenced and typed.

    Journal:

    Article Title: Detection and genotyping of Human Papillomaviruses from archival formalin fixed tissue samples

    doi: 10.1002/cpmc.16

    Figure Lengend Snippet: Flow chart of the different methods in the Protocol Following DNA extraction, the Protocol describes three complementary methods aimed at PCR amplifying fragments from the papillomavirus L1 open reading frame. Following PCR, the amplicons are sequenced and typed.

    Article Snippet: 11732668001) Agarose Gel purification kit (e.g. zymoclean Gel DNA recovery kit; Zymo. cat. no. D4007) 10X ThermoPol Buffer (NEB cat. no. B9004) 10 mM dATP Taq DNA Polymerase (NEB cat. no. M0267) 200 μl thin-walled PCR tubes (or other appropriate for thermal cycler) TOPO TA cloning kit (ThermoFisher cat. no. K450001) Topoisomerase I-activated pCR™2.1-TOPO® vector Salt solution (1.2 M NaCl and 0.06 M MgCl2 ) M13 forward and reverse primers One Shot Chemically Competent E. coli (TOP10; F– mcr A Δ( mrr - hsd RMS- mcr BC) Φ80 lac ZΔM15 Δ lac X74 rec A1 ara D139 Δ( ara leu ) 7697 gal U gal K rps L (StrR) end A1 nup G) S.O.C. medium (see recipe) LB plates containing 50 μg/ml Kanamycin or Carbenicillin Miniprep kit (e.g. Wizard Plus SV Miniprep; Promega, cat. no. A1270) Thermal cycler

    Techniques: Flow Cytometry, DNA Extraction, Polymerase Chain Reaction