thiamine pyrophosphate Search Results


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  • 99
    Millipore thiamine pyrophosphate
    Exposure of P. aeruginosa biofilms to active pyruvate dehydrogenase (PDH) coincides with a reduction in the biofilm biomass in a manner independent of biofilm age. Biofilms were grown for 4 days in 24-well polystyrene plates in five-fold diluted LB. ( A ) Remaining biofilm biomass following exposure to 5, 10, and 20 mU PDH, as determined using CV staining. Inset, CV-stained biofilms prior to and post treatment with PDH. ( B ) Absorbance of biofilm supernatant following exposure to PDH or heat-inactivated PDH. ( C ) Brightfield images of biofilms grown for 3, 6, and 7 days prior to and post treatment with 10 mU PDH. ( D ) Biofilm biomass following exposure to heat-inactivated PDH. Remaining biofilm biomass following exposure to ( E ) 10 mM lactate or cofactors and products of the PDH catalyzed reaction, namely ß-NAD + and <t>acetyl-CoA,</t> ß-NADH, and 10 mM nitrate, or to ( F ) increasing concentration of pyruvate (0, 1, 10, and 100 mM) in the presence and absence of PDH. Untreated biofilms were used as controls. PDH treatment was done in the presence of CoA, ß-NAD + , <t>TPP,</t> and MgSO 4. *Significantly different (p
    Thiamine Pyrophosphate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 344 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thiamine pyrophosphate/product/Millipore
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    90
    Millipore thiamin diphosphate
    Exposure of P. aeruginosa biofilms to active pyruvate dehydrogenase (PDH) coincides with a reduction in the biofilm biomass in a manner independent of biofilm age. Biofilms were grown for 4 days in 24-well polystyrene plates in five-fold diluted LB. ( A ) Remaining biofilm biomass following exposure to 5, 10, and 20 mU PDH, as determined using CV staining. Inset, CV-stained biofilms prior to and post treatment with PDH. ( B ) Absorbance of biofilm supernatant following exposure to PDH or heat-inactivated PDH. ( C ) Brightfield images of biofilms grown for 3, 6, and 7 days prior to and post treatment with 10 mU PDH. ( D ) Biofilm biomass following exposure to heat-inactivated PDH. Remaining biofilm biomass following exposure to ( E ) 10 mM lactate or cofactors and products of the PDH catalyzed reaction, namely ß-NAD + and <t>acetyl-CoA,</t> ß-NADH, and 10 mM nitrate, or to ( F ) increasing concentration of pyruvate (0, 1, 10, and 100 mM) in the presence and absence of PDH. Untreated biofilms were used as controls. PDH treatment was done in the presence of CoA, ß-NAD + , <t>TPP,</t> and MgSO 4. *Significantly different (p
    Thiamin Diphosphate, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thiamin diphosphate/product/Millipore
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    90
    FUJIFILM thiamine pyrophosphate
    Exposure of P. aeruginosa biofilms to active pyruvate dehydrogenase (PDH) coincides with a reduction in the biofilm biomass in a manner independent of biofilm age. Biofilms were grown for 4 days in 24-well polystyrene plates in five-fold diluted LB. ( A ) Remaining biofilm biomass following exposure to 5, 10, and 20 mU PDH, as determined using CV staining. Inset, CV-stained biofilms prior to and post treatment with PDH. ( B ) Absorbance of biofilm supernatant following exposure to PDH or heat-inactivated PDH. ( C ) Brightfield images of biofilms grown for 3, 6, and 7 days prior to and post treatment with 10 mU PDH. ( D ) Biofilm biomass following exposure to heat-inactivated PDH. Remaining biofilm biomass following exposure to ( E ) 10 mM lactate or cofactors and products of the PDH catalyzed reaction, namely ß-NAD + and <t>acetyl-CoA,</t> ß-NADH, and 10 mM nitrate, or to ( F ) increasing concentration of pyruvate (0, 1, 10, and 100 mM) in the presence and absence of PDH. Untreated biofilms were used as controls. PDH treatment was done in the presence of CoA, ß-NAD + , <t>TPP,</t> and MgSO 4. *Significantly different (p
    Thiamine Pyrophosphate, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Moravek Biochemicals h thiamin pyrophosphate tpp
    Exposure of P. aeruginosa biofilms to active pyruvate dehydrogenase (PDH) coincides with a reduction in the biofilm biomass in a manner independent of biofilm age. Biofilms were grown for 4 days in 24-well polystyrene plates in five-fold diluted LB. ( A ) Remaining biofilm biomass following exposure to 5, 10, and 20 mU PDH, as determined using CV staining. Inset, CV-stained biofilms prior to and post treatment with PDH. ( B ) Absorbance of biofilm supernatant following exposure to PDH or heat-inactivated PDH. ( C ) Brightfield images of biofilms grown for 3, 6, and 7 days prior to and post treatment with 10 mU PDH. ( D ) Biofilm biomass following exposure to heat-inactivated PDH. Remaining biofilm biomass following exposure to ( E ) 10 mM lactate or cofactors and products of the PDH catalyzed reaction, namely ß-NAD + and <t>acetyl-CoA,</t> ß-NADH, and 10 mM nitrate, or to ( F ) increasing concentration of pyruvate (0, 1, 10, and 100 mM) in the presence and absence of PDH. Untreated biofilms were used as controls. PDH treatment was done in the presence of CoA, ß-NAD + , <t>TPP,</t> and MgSO 4. *Significantly different (p
    H Thiamin Pyrophosphate Tpp, supplied by Moravek Biochemicals, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher thiamine pyrophosphate chloride
    Exposure of P. aeruginosa biofilms to active pyruvate dehydrogenase (PDH) coincides with a reduction in the biofilm biomass in a manner independent of biofilm age. Biofilms were grown for 4 days in 24-well polystyrene plates in five-fold diluted LB. ( A ) Remaining biofilm biomass following exposure to 5, 10, and 20 mU PDH, as determined using CV staining. Inset, CV-stained biofilms prior to and post treatment with PDH. ( B ) Absorbance of biofilm supernatant following exposure to PDH or heat-inactivated PDH. ( C ) Brightfield images of biofilms grown for 3, 6, and 7 days prior to and post treatment with 10 mU PDH. ( D ) Biofilm biomass following exposure to heat-inactivated PDH. Remaining biofilm biomass following exposure to ( E ) 10 mM lactate or cofactors and products of the PDH catalyzed reaction, namely ß-NAD + and <t>acetyl-CoA,</t> ß-NADH, and 10 mM nitrate, or to ( F ) increasing concentration of pyruvate (0, 1, 10, and 100 mM) in the presence and absence of PDH. Untreated biofilms were used as controls. PDH treatment was done in the presence of CoA, ß-NAD + , <t>TPP,</t> and MgSO 4. *Significantly different (p
    Thiamine Pyrophosphate Chloride, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ARUP Laboratories thiamine diphosphate vitamin b1 levels
    Exposure of P. aeruginosa biofilms to active pyruvate dehydrogenase (PDH) coincides with a reduction in the biofilm biomass in a manner independent of biofilm age. Biofilms were grown for 4 days in 24-well polystyrene plates in five-fold diluted LB. ( A ) Remaining biofilm biomass following exposure to 5, 10, and 20 mU PDH, as determined using CV staining. Inset, CV-stained biofilms prior to and post treatment with PDH. ( B ) Absorbance of biofilm supernatant following exposure to PDH or heat-inactivated PDH. ( C ) Brightfield images of biofilms grown for 3, 6, and 7 days prior to and post treatment with 10 mU PDH. ( D ) Biofilm biomass following exposure to heat-inactivated PDH. Remaining biofilm biomass following exposure to ( E ) 10 mM lactate or cofactors and products of the PDH catalyzed reaction, namely ß-NAD + and <t>acetyl-CoA,</t> ß-NADH, and 10 mM nitrate, or to ( F ) increasing concentration of pyruvate (0, 1, 10, and 100 mM) in the presence and absence of PDH. Untreated biofilms were used as controls. PDH treatment was done in the presence of CoA, ß-NAD + , <t>TPP,</t> and MgSO 4. *Significantly different (p
    Thiamine Diphosphate Vitamin B1 Levels, supplied by ARUP Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    ICN Biomedicals thiamine diphosphate tpp
    Exposure of P. aeruginosa biofilms to active pyruvate dehydrogenase (PDH) coincides with a reduction in the biofilm biomass in a manner independent of biofilm age. Biofilms were grown for 4 days in 24-well polystyrene plates in five-fold diluted LB. ( A ) Remaining biofilm biomass following exposure to 5, 10, and 20 mU PDH, as determined using CV staining. Inset, CV-stained biofilms prior to and post treatment with PDH. ( B ) Absorbance of biofilm supernatant following exposure to PDH or heat-inactivated PDH. ( C ) Brightfield images of biofilms grown for 3, 6, and 7 days prior to and post treatment with 10 mU PDH. ( D ) Biofilm biomass following exposure to heat-inactivated PDH. Remaining biofilm biomass following exposure to ( E ) 10 mM lactate or cofactors and products of the PDH catalyzed reaction, namely ß-NAD + and <t>acetyl-CoA,</t> ß-NADH, and 10 mM nitrate, or to ( F ) increasing concentration of pyruvate (0, 1, 10, and 100 mM) in the presence and absence of PDH. Untreated biofilms were used as controls. PDH treatment was done in the presence of CoA, ß-NAD + , <t>TPP,</t> and MgSO 4. *Significantly different (p
    Thiamine Diphosphate Tpp, supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Merck & Co thiamine pyrophosphate tpp
    Exposure of P. aeruginosa biofilms to active pyruvate dehydrogenase (PDH) coincides with a reduction in the biofilm biomass in a manner independent of biofilm age. Biofilms were grown for 4 days in 24-well polystyrene plates in five-fold diluted LB. ( A ) Remaining biofilm biomass following exposure to 5, 10, and 20 mU PDH, as determined using CV staining. Inset, CV-stained biofilms prior to and post treatment with PDH. ( B ) Absorbance of biofilm supernatant following exposure to PDH or heat-inactivated PDH. ( C ) Brightfield images of biofilms grown for 3, 6, and 7 days prior to and post treatment with 10 mU PDH. ( D ) Biofilm biomass following exposure to heat-inactivated PDH. Remaining biofilm biomass following exposure to ( E ) 10 mM lactate or cofactors and products of the PDH catalyzed reaction, namely ß-NAD + and <t>acetyl-CoA,</t> ß-NADH, and 10 mM nitrate, or to ( F ) increasing concentration of pyruvate (0, 1, 10, and 100 mM) in the presence and absence of PDH. Untreated biofilms were used as controls. PDH treatment was done in the presence of CoA, ß-NAD + , <t>TPP,</t> and MgSO 4. *Significantly different (p
    Thiamine Pyrophosphate Tpp, supplied by Merck & Co, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Sangon Biotech thiamine pyrophosphate tpp
    Exposure of P. aeruginosa biofilms to active pyruvate dehydrogenase (PDH) coincides with a reduction in the biofilm biomass in a manner independent of biofilm age. Biofilms were grown for 4 days in 24-well polystyrene plates in five-fold diluted LB. ( A ) Remaining biofilm biomass following exposure to 5, 10, and 20 mU PDH, as determined using CV staining. Inset, CV-stained biofilms prior to and post treatment with PDH. ( B ) Absorbance of biofilm supernatant following exposure to PDH or heat-inactivated PDH. ( C ) Brightfield images of biofilms grown for 3, 6, and 7 days prior to and post treatment with 10 mU PDH. ( D ) Biofilm biomass following exposure to heat-inactivated PDH. Remaining biofilm biomass following exposure to ( E ) 10 mM lactate or cofactors and products of the PDH catalyzed reaction, namely ß-NAD + and <t>acetyl-CoA,</t> ß-NADH, and 10 mM nitrate, or to ( F ) increasing concentration of pyruvate (0, 1, 10, and 100 mM) in the presence and absence of PDH. Untreated biofilms were used as controls. PDH treatment was done in the presence of CoA, ß-NAD + , <t>TPP,</t> and MgSO 4. *Significantly different (p
    Thiamine Pyrophosphate Tpp, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Exposure of P. aeruginosa biofilms to active pyruvate dehydrogenase (PDH) coincides with a reduction in the biofilm biomass in a manner independent of biofilm age. Biofilms were grown for 4 days in 24-well polystyrene plates in five-fold diluted LB. ( A ) Remaining biofilm biomass following exposure to 5, 10, and 20 mU PDH, as determined using CV staining. Inset, CV-stained biofilms prior to and post treatment with PDH. ( B ) Absorbance of biofilm supernatant following exposure to PDH or heat-inactivated PDH. ( C ) Brightfield images of biofilms grown for 3, 6, and 7 days prior to and post treatment with 10 mU PDH. ( D ) Biofilm biomass following exposure to heat-inactivated PDH. Remaining biofilm biomass following exposure to ( E ) 10 mM lactate or cofactors and products of the PDH catalyzed reaction, namely ß-NAD + and acetyl-CoA, ß-NADH, and 10 mM nitrate, or to ( F ) increasing concentration of pyruvate (0, 1, 10, and 100 mM) in the presence and absence of PDH. Untreated biofilms were used as controls. PDH treatment was done in the presence of CoA, ß-NAD + , TPP, and MgSO 4. *Significantly different (p

    Journal: Scientific Reports

    Article Title: Pyruvate-depleting conditions induce biofilm dispersion and enhance the efficacy of antibiotics in killing biofilms in vitro and in vivo

    doi: 10.1038/s41598-019-40378-z

    Figure Lengend Snippet: Exposure of P. aeruginosa biofilms to active pyruvate dehydrogenase (PDH) coincides with a reduction in the biofilm biomass in a manner independent of biofilm age. Biofilms were grown for 4 days in 24-well polystyrene plates in five-fold diluted LB. ( A ) Remaining biofilm biomass following exposure to 5, 10, and 20 mU PDH, as determined using CV staining. Inset, CV-stained biofilms prior to and post treatment with PDH. ( B ) Absorbance of biofilm supernatant following exposure to PDH or heat-inactivated PDH. ( C ) Brightfield images of biofilms grown for 3, 6, and 7 days prior to and post treatment with 10 mU PDH. ( D ) Biofilm biomass following exposure to heat-inactivated PDH. Remaining biofilm biomass following exposure to ( E ) 10 mM lactate or cofactors and products of the PDH catalyzed reaction, namely ß-NAD + and acetyl-CoA, ß-NADH, and 10 mM nitrate, or to ( F ) increasing concentration of pyruvate (0, 1, 10, and 100 mM) in the presence and absence of PDH. Untreated biofilms were used as controls. PDH treatment was done in the presence of CoA, ß-NAD + , TPP, and MgSO 4. *Significantly different (p

    Article Snippet: Briefly, biofilms were grown for up to 5 days prior to the addition of 1–20 mU PDH in the presence of 2 mM CoA (Sigma), 2 mM ß-NAD+ (Sigma), 20 µM thiamine pyrophosphate (TPP) (Sigma), and 50 µM magnesium sulfate (MgSO4 ) in 5-fold diluted LB or BHI.

    Techniques: Staining, Concentration Assay

    Pharmacokinetic profiles of thiamine, TMP and TDP do not differ between wild-type and Pap −/− mice following oral administration of BT. ( A–F ) BT (0–300 mg/kg, p.o.) was administered to wild-type and Pap −/− mice (n = 5/dose). The indicated tissues were harvested 1 h post oral gavage then were homogenized in 10% trichloroacetic acid. Fluorescent thiochrome derivatives were monitored by reversed phase HPLC to detect ( A–B ) thiamine, ( C–D ) TMP, and ( E–F ) TDP. Peak area values were converted to units of concentration for each thiamine derivative and normalized by the weight of tissue in each replicate. There were no significant differences between wild-type and Pap −/− mice. Data are plotted as means ± s.e.m.

    Journal: PLoS ONE

    Article Title: Prostatic Acid Phosphatase Is Required for the Antinociceptive Effects of Thiamine and Benfotiamine

    doi: 10.1371/journal.pone.0048562

    Figure Lengend Snippet: Pharmacokinetic profiles of thiamine, TMP and TDP do not differ between wild-type and Pap −/− mice following oral administration of BT. ( A–F ) BT (0–300 mg/kg, p.o.) was administered to wild-type and Pap −/− mice (n = 5/dose). The indicated tissues were harvested 1 h post oral gavage then were homogenized in 10% trichloroacetic acid. Fluorescent thiochrome derivatives were monitored by reversed phase HPLC to detect ( A–B ) thiamine, ( C–D ) TMP, and ( E–F ) TDP. Peak area values were converted to units of concentration for each thiamine derivative and normalized by the weight of tissue in each replicate. There were no significant differences between wild-type and Pap −/− mice. Data are plotted as means ± s.e.m.

    Article Snippet: BT (from stock, made as described above), thiamine monophosphate hydrochloride (TMP), thiamine hydrochloride, thiamine pyrophosphate (TDP) and thiochrome were purchased from Sigma-Aldrich and diluted in 0.9% saline.

    Techniques: Mouse Assay, High Performance Liquid Chromatography, Concentration Assay

    TMP, but not other thiamine metabolites, inhibits noxious thermal sensitivity in a PAP-dependent manner. ( A–C ) Wild-type and Pap −/− male mice were tested for noxious thermal sensitivity (hindpaw, radiant heating) before (baseline, BL) and following i.t. injection of ( A ) 25 nmol TDP (n = 10 wild-type mice, n = 9 Pap −/− mice), ( B ) 100 nmol TMP (n = 17 wild-type mice, n = 18 Pap −/− mice) and ( C ) 25 nmol thiochrome (n = 10 wild-type mice, n = 9 Pap −/− mice). ( A–C ) T tests were used to compare responses at each time point to baseline (BL). * P

    Journal: PLoS ONE

    Article Title: Prostatic Acid Phosphatase Is Required for the Antinociceptive Effects of Thiamine and Benfotiamine

    doi: 10.1371/journal.pone.0048562

    Figure Lengend Snippet: TMP, but not other thiamine metabolites, inhibits noxious thermal sensitivity in a PAP-dependent manner. ( A–C ) Wild-type and Pap −/− male mice were tested for noxious thermal sensitivity (hindpaw, radiant heating) before (baseline, BL) and following i.t. injection of ( A ) 25 nmol TDP (n = 10 wild-type mice, n = 9 Pap −/− mice), ( B ) 100 nmol TMP (n = 17 wild-type mice, n = 18 Pap −/− mice) and ( C ) 25 nmol thiochrome (n = 10 wild-type mice, n = 9 Pap −/− mice). ( A–C ) T tests were used to compare responses at each time point to baseline (BL). * P

    Article Snippet: BT (from stock, made as described above), thiamine monophosphate hydrochloride (TMP), thiamine hydrochloride, thiamine pyrophosphate (TDP) and thiochrome were purchased from Sigma-Aldrich and diluted in 0.9% saline.

    Techniques: Mouse Assay, Injection