thermocycler Thermo Fisher Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 84
    Thermo Fisher thermo fisher scientific thermocyclers
    Thermo Fisher Scientific Thermocyclers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermo fisher scientific thermocyclers/product/Thermo Fisher
    Average 84 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    thermo fisher scientific thermocyclers - by Bioz Stars, 2020-02
    84/100 stars
      Buy from Supplier

    86
    Thermo Fisher stratagene thermocycler
    Stratagene Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stratagene thermocycler/product/Thermo Fisher
    Average 86 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    stratagene thermocycler - by Bioz Stars, 2020-02
    86/100 stars
      Buy from Supplier

    99
    Thermo Fisher thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4866 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Thermo Fisher
    Average 99 stars, based on 4866 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    80
    Thermo Fisher quantstudio3 thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Quantstudio3 Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantstudio3 thermocycler/product/Thermo Fisher
    Average 80 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    quantstudio3 thermocycler - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    81
    Thermo Fisher thermocycler veriti
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermocycler Veriti, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler veriti/product/Thermo Fisher
    Average 81 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    thermocycler veriti - by Bioz Stars, 2020-02
    81/100 stars
      Buy from Supplier

    90
    Thermo Fisher a stepone thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    A Stepone Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a stepone thermocycler/product/Thermo Fisher
    Average 90 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    a stepone thermocycler - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    81
    Thermo Fisher thermohybrid thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermohybrid Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermohybrid thermocycler/product/Thermo Fisher
    Average 81 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    thermohybrid thermocycler - by Bioz Stars, 2020-02
    81/100 stars
      Buy from Supplier

    95
    Thermo Fisher geneamp 2700 thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Geneamp 2700 Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/geneamp 2700 thermocycler/product/Thermo Fisher
    Average 95 stars, based on 185 article reviews
    Price from $9.99 to $1999.99
    geneamp 2700 thermocycler - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    81
    Thermo Fisher geneamp 9600 thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Geneamp 9600 Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/geneamp 9600 thermocycler/product/Thermo Fisher
    Average 81 stars, based on 84 article reviews
    Price from $9.99 to $1999.99
    geneamp 9600 thermocycler - by Bioz Stars, 2020-02
    81/100 stars
      Buy from Supplier

    78
    Thermo Fisher pico thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Pico Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pico thermocycler/product/Thermo Fisher
    Average 78 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pico thermocycler - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    79
    Thermo Fisher stratagene mx3000 thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Stratagene Mx3000 Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stratagene mx3000 thermocycler/product/Thermo Fisher
    Average 79 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    stratagene mx3000 thermocycler - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    89
    Thermo Fisher biometra professional thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Biometra Professional Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biometra professional thermocycler/product/Thermo Fisher
    Average 89 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    biometra professional thermocycler - by Bioz Stars, 2020-02
    89/100 stars
      Buy from Supplier

    82
    Thermo Fisher stratagene mx3005p thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Stratagene Mx3005p Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stratagene mx3005p thermocycler/product/Thermo Fisher
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    stratagene mx3005p thermocycler - by Bioz Stars, 2020-02
    82/100 stars
      Buy from Supplier

    80
    Thermo Fisher model db66p25 thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Model Db66p25 Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/model db66p25 thermocycler/product/Thermo Fisher
    Average 80 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    model db66p25 thermocycler - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    79
    Thermo Fisher px2 system thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Px2 System Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/px2 system thermocycler/product/Thermo Fisher
    Average 79 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    px2 system thermocycler - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    84
    Thermo Fisher prism 7500ht thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Prism 7500ht Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prism 7500ht thermocycler/product/Thermo Fisher
    Average 84 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    prism 7500ht thermocycler - by Bioz Stars, 2020-02
    84/100 stars
      Buy from Supplier

    91
    Thermo Fisher arktik thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Arktik Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arktik thermocycler/product/Thermo Fisher
    Average 91 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    arktik thermocycler - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

    78
    Thermo Fisher techne cyclogene thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Techne Cyclogene Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/techne cyclogene thermocycler/product/Thermo Fisher
    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    techne cyclogene thermocycler - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    79
    Thermo Fisher abi 7300 thermocycler system
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Abi 7300 Thermocycler System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi 7300 thermocycler system/product/Thermo Fisher
    Average 79 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    abi 7300 thermocycler system - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    76
    Thermo Fisher px 2 thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Px 2 Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/px 2 thermocycler/product/Thermo Fisher
    Average 76 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    px 2 thermocycler - by Bioz Stars, 2020-02
    76/100 stars
      Buy from Supplier

    93
    Thermo Fisher viia 7 thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Viia 7 Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/viia 7 thermocycler/product/Thermo Fisher
    Average 93 stars, based on 183 article reviews
    Price from $9.99 to $1999.99
    viia 7 thermocycler - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    92
    Thermo Fisher abi 7000 thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Abi 7000 Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi 7000 thermocycler/product/Thermo Fisher
    Average 92 stars, based on 206 article reviews
    Price from $9.99 to $1999.99
    abi 7000 thermocycler - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

    99
    Thermo Fisher steponeplus thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Steponeplus Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 762 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/steponeplus thermocycler/product/Thermo Fisher
    Average 99 stars, based on 762 article reviews
    Price from $9.99 to $1999.99
    steponeplus thermocycler - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    84
    Thermo Fisher piko real 96 thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Piko Real 96 Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/piko real 96 thermocycler/product/Thermo Fisher
    Average 84 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    piko real 96 thermocycler - by Bioz Stars, 2020-02
    84/100 stars
      Buy from Supplier

    78
    Thermo Fisher perkinelmer 9700 thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Perkinelmer 9700 Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/perkinelmer 9700 thermocycler/product/Thermo Fisher
    Average 78 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    perkinelmer 9700 thermocycler - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    86
    Thermo Fisher thermo hybaid thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermo Hybaid Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermo hybaid thermocycler/product/Thermo Fisher
    Average 86 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    thermo hybaid thermocycler - by Bioz Stars, 2020-02
    86/100 stars
      Buy from Supplier

    94
    Thermo Fisher abi prism 7500 thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Abi Prism 7500 Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism 7500 thermocycler/product/Thermo Fisher
    Average 94 stars, based on 141 article reviews
    Price from $9.99 to $1999.99
    abi prism 7500 thermocycler - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    80
    Thermo Fisher thermocycler abi 7500
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermocycler Abi 7500, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler abi 7500/product/Thermo Fisher
    Average 80 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    thermocycler abi 7500 - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    82
    Thermo Fisher 7500 thermocycler system
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    7500 Thermocycler System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7500 thermocycler system/product/Thermo Fisher
    Average 82 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    7500 thermocycler system - by Bioz Stars, 2020-02
    82/100 stars
      Buy from Supplier

    84
    Thermo Fisher picoreal thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Picoreal Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/picoreal thermocycler/product/Thermo Fisher
    Average 84 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    picoreal thermocycler - by Bioz Stars, 2020-02
    84/100 stars
      Buy from Supplier

    76
    Thermo Fisher abi thermocycler system
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Abi Thermocycler System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi thermocycler system/product/Thermo Fisher
    Average 76 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    abi thermocycler system - by Bioz Stars, 2020-02
    76/100 stars
      Buy from Supplier

    77
    Thermo Fisher applied biosystems verti thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Applied Biosystems Verti Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/applied biosystems verti thermocycler/product/Thermo Fisher
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    applied biosystems verti thermocycler - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    Image Search Results


    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same thermocycler and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.

    Journal: Scientific Reports

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest

    doi: 10.1038/s41598-017-18216-x

    Figure Lengend Snippet: Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same thermocycler and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.

    Article Snippet: The PCR assays were performed in a thermocycler (Veriti 96 wells, Applied Biosystems) with the following cycling parameters: an initial denaturation at 94 °C for 2 min followed by 40 cycles of denaturation at 94 °C for 30 sec, annealing at 54 °C for 20 sec and extension at 72 °C for 30 sec followed by a final extension incubation at 72 °C for 2 min.

    Techniques: Nested PCR, Polymerase Chain Reaction, Negative Control

    Differential diagnosis of Plasmodium simium infection by nested/PCR followed by a digestion with Hpy CH4III restriction enzyme of 9 infected human samples from Atlantic Forest in Rio de Janeiro/RJ (H2 – H9) and one from Amazon endemic region (H1) according to Additional file 1. 3% agarose gel stained with ethidium bromide. MM:1 kb Plus Ladder (ThermoFischer, Calrsbad, CA, USA). Reactions were performed simultaneously in the same thermocycler and splited in different gels.D: Digested; ND: Non Digested. PC Pv: Positive Control for P. vivax , PC Ps: positive control for P. simium . NC: Negative Control (without DNA).

    Journal: Scientific Reports

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest

    doi: 10.1038/s41598-017-18216-x

    Figure Lengend Snippet: Differential diagnosis of Plasmodium simium infection by nested/PCR followed by a digestion with Hpy CH4III restriction enzyme of 9 infected human samples from Atlantic Forest in Rio de Janeiro/RJ (H2 – H9) and one from Amazon endemic region (H1) according to Additional file 1. 3% agarose gel stained with ethidium bromide. MM:1 kb Plus Ladder (ThermoFischer, Calrsbad, CA, USA). Reactions were performed simultaneously in the same thermocycler and splited in different gels.D: Digested; ND: Non Digested. PC Pv: Positive Control for P. vivax , PC Ps: positive control for P. simium . NC: Negative Control (without DNA).

    Article Snippet: The PCR assays were performed in a thermocycler (Veriti 96 wells, Applied Biosystems) with the following cycling parameters: an initial denaturation at 94 °C for 2 min followed by 40 cycles of denaturation at 94 °C for 30 sec, annealing at 54 °C for 20 sec and extension at 72 °C for 30 sec followed by a final extension incubation at 72 °C for 2 min.

    Techniques: Infection, Nested PCR, Agarose Gel Electrophoresis, Staining, Positive Control, Negative Control

    Nested/PCR-RFLP of P. vivax DNA samples. 15 DNA samples from P. vivax infected individuals from different parts of Amazon: Porto Velho/Rondônia State, Brazil (PvPV/RO1 and 2), Guyana (PvGuy), Ariquemes/Rondônia State, Brazil (PvAri/RO), Venezuela (PvVen), French Guiana (PvFrGui), Novo progresso/Pará State, Brazil (PvNP/PA), Rio Pardo/Amazonas State, Brazil (PvRP/AM1, 2 and 3), Humaita/Amazonas State, Brazil (PvHu/Am1, 2, 3 and 4) and unknown city in Amazonas State, Brazil (PvAM) were used for Nested PCR amplification followed by digestion with Hpy CH4III restriction enzyme. 3% Agarose gel stained with ethidium bromide. Name tags above gels indicated the patients according to Additional file 2. Reactions were performed simultaneously in the same thermocycler and splited in different gels. MM: 1 kb Plus Ladder. D: Digested (8 μL of digestion); ND: Non Digested (the equivalent amount of PCR product used in the digestion, 6.5 μL of samples and 5 μL of controls); PC Pv: positive control of P. vivax (pool of samples from infected patient from Amazonia); PC Ps: positive control of P. simium ( Alouatta g. clamitans infected with P. simium ); NC: negative Control.

    Journal: Scientific Reports

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest

    doi: 10.1038/s41598-017-18216-x

    Figure Lengend Snippet: Nested/PCR-RFLP of P. vivax DNA samples. 15 DNA samples from P. vivax infected individuals from different parts of Amazon: Porto Velho/Rondônia State, Brazil (PvPV/RO1 and 2), Guyana (PvGuy), Ariquemes/Rondônia State, Brazil (PvAri/RO), Venezuela (PvVen), French Guiana (PvFrGui), Novo progresso/Pará State, Brazil (PvNP/PA), Rio Pardo/Amazonas State, Brazil (PvRP/AM1, 2 and 3), Humaita/Amazonas State, Brazil (PvHu/Am1, 2, 3 and 4) and unknown city in Amazonas State, Brazil (PvAM) were used for Nested PCR amplification followed by digestion with Hpy CH4III restriction enzyme. 3% Agarose gel stained with ethidium bromide. Name tags above gels indicated the patients according to Additional file 2. Reactions were performed simultaneously in the same thermocycler and splited in different gels. MM: 1 kb Plus Ladder. D: Digested (8 μL of digestion); ND: Non Digested (the equivalent amount of PCR product used in the digestion, 6.5 μL of samples and 5 μL of controls); PC Pv: positive control of P. vivax (pool of samples from infected patient from Amazonia); PC Ps: positive control of P. simium ( Alouatta g. clamitans infected with P. simium ); NC: negative Control.

    Article Snippet: The PCR assays were performed in a thermocycler (Veriti 96 wells, Applied Biosystems) with the following cycling parameters: an initial denaturation at 94 °C for 2 min followed by 40 cycles of denaturation at 94 °C for 30 sec, annealing at 54 °C for 20 sec and extension at 72 °C for 30 sec followed by a final extension incubation at 72 °C for 2 min.

    Techniques: Nested PCR, Infection, Amplification, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction, Positive Control, Negative Control

    Differential Diagnosis of Plasmodium simium infection by nested/PCR followed by a digestion with Hpy CH4III restriction enzyme of 16 non-human primate samples: ( A ) 2 captive NHP from Rio de Janeiro/RJ ( Sapajus xanthosternos 2098; Cacajao melanocephalus 2302), one free-living Alouatta g. clamitans from Rio de Janeiro State (3636) and 6 free-living Alouatta g. clamitans from Joinville/SC, Brazil (J9, J11, J15, J20, J22, J25); ( B ) 7 Alouatta g. clamitans from CEPESBI, Indaial, SC, Brazil (Bl3, Bl6, Bl10, Bl28, Bl61, Bl64, Bl69), *Captive NHPs, all the other were free-living. More details about each sample see Additional file 1. 3% agarose gel stained with ethidium bromide. MM:1 kb Plus Ladder (ThermoFischer). Reactions were performed simultaneously in the same thermocycler and splited in different gels. PC Pv: Positive Control for P. vivax , PC Ps: positive control for P. simium . NC: Negative Control (without DNA).

    Journal: Scientific Reports

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest

    doi: 10.1038/s41598-017-18216-x

    Figure Lengend Snippet: Differential Diagnosis of Plasmodium simium infection by nested/PCR followed by a digestion with Hpy CH4III restriction enzyme of 16 non-human primate samples: ( A ) 2 captive NHP from Rio de Janeiro/RJ ( Sapajus xanthosternos 2098; Cacajao melanocephalus 2302), one free-living Alouatta g. clamitans from Rio de Janeiro State (3636) and 6 free-living Alouatta g. clamitans from Joinville/SC, Brazil (J9, J11, J15, J20, J22, J25); ( B ) 7 Alouatta g. clamitans from CEPESBI, Indaial, SC, Brazil (Bl3, Bl6, Bl10, Bl28, Bl61, Bl64, Bl69), *Captive NHPs, all the other were free-living. More details about each sample see Additional file 1. 3% agarose gel stained with ethidium bromide. MM:1 kb Plus Ladder (ThermoFischer). Reactions were performed simultaneously in the same thermocycler and splited in different gels. PC Pv: Positive Control for P. vivax , PC Ps: positive control for P. simium . NC: Negative Control (without DNA).

    Article Snippet: The PCR assays were performed in a thermocycler (Veriti 96 wells, Applied Biosystems) with the following cycling parameters: an initial denaturation at 94 °C for 2 min followed by 40 cycles of denaturation at 94 °C for 30 sec, annealing at 54 °C for 20 sec and extension at 72 °C for 30 sec followed by a final extension incubation at 72 °C for 2 min.

    Techniques: Infection, Nested PCR, Agarose Gel Electrophoresis, Staining, Positive Control, Negative Control

    Melting curve analyses of the PCR products obtained from the amplification of the rtxA gene of Kingella kingae with the ABI PRISM 7000 (Applied Biosystems) (A), the SmartCycler (Cepheid) (B), and the LightCycler (Roche Diagnostics) (C). The temperature

    Journal: Journal of Clinical Microbiology

    Article Title: The rtxA Toxin Gene of Kingella kingae: a Pertinent Target for Molecular Diagnosis of Osteoarticular Infections ▿: a Pertinent Target for Molecular Diagnosis of Osteoarticular Infections ▿ †

    doi: 10.1128/JCM.01657-10

    Figure Lengend Snippet: Melting curve analyses of the PCR products obtained from the amplification of the rtxA gene of Kingella kingae with the ABI PRISM 7000 (Applied Biosystems) (A), the SmartCycler (Cepheid) (B), and the LightCycler (Roche Diagnostics) (C). The temperature

    Article Snippet: SYBR green real-time PCR amplification and melting curve analysis (MCA) were carried out with a final volume of 25 μl containing 12.5 μl of SYBR green PCR master mix (Applied Biosystems), 0.3 μM each primer, and 5 μl of purified DNA in an ABI PRISM 7000 thermocycler (Applied Biosystems).

    Techniques: Polymerase Chain Reaction, Amplification