thermocycler Roche Search Results


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  • 99
    Thermo Fisher power sybr green master mix
    Power Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher mgcl2
    Mgcl2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 104037 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher trizol reagent
    Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 635164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher rna
    Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 177224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher thermocycler
    Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 5940 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Eppendorf AG thermocycler
    Thermocycler, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 94/100, based on 4419 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher total rna
    VCIP 5′UTR does not contain cryptic promoter activity or evidence of spurious splicing. (A) <t>U2OS</t> cells were transfected with the β-Gal/EMPTY/CAT, β-Gal/VCIP/CAT, or HP-β-Gal/VCIP/CAT bicistronic plasmid. β-Gal and CAT activity was measured from cell lysates taken 24 h posttransfection and normalized to the number of transfected cells based on neomycin activity. Reported β-Gal and CAT activities are expressed relative to measurements obtained for β-Gal/EMPTY/CAT. Bars represent the averages ± SEM from three independent experiments. (B) Quantitative RT-PCR was performed with total <t>RNA</t> isolated from U2OS cells transfected with the β-Gal/EMCV/CAT, β-Gal/EMPTY/CAT, or β-Gal/VCIP/CAT bicistronic plasmid. Q-PCRs were carried out to quantify the number of β-Gal and CAT cistrons expressed in each plasmid. The CAT/β-Gal ratio was calculated as 2 −[Ct(CAT) − Ct(β-Gal)] , where Ct is the crossing point. The bars represent the means ± SEM from six to seven independent experiments performed in triplicate.
    Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 471882 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa sybr premix ex taq ii
    VCIP 5′UTR does not contain cryptic promoter activity or evidence of spurious splicing. (A) <t>U2OS</t> cells were transfected with the β-Gal/EMPTY/CAT, β-Gal/VCIP/CAT, or HP-β-Gal/VCIP/CAT bicistronic plasmid. β-Gal and CAT activity was measured from cell lysates taken 24 h posttransfection and normalized to the number of transfected cells based on neomycin activity. Reported β-Gal and CAT activities are expressed relative to measurements obtained for β-Gal/EMPTY/CAT. Bars represent the averages ± SEM from three independent experiments. (B) Quantitative RT-PCR was performed with total <t>RNA</t> isolated from U2OS cells transfected with the β-Gal/EMCV/CAT, β-Gal/EMPTY/CAT, or β-Gal/VCIP/CAT bicistronic plasmid. Q-PCRs were carried out to quantify the number of β-Gal and CAT cistrons expressed in each plasmid. The CAT/β-Gal ratio was calculated as 2 −[Ct(CAT) − Ct(β-Gal)] , where Ct is the crossing point. The bars represent the means ± SEM from six to seven independent experiments performed in triplicate.
    Sybr Premix Ex Taq Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 29794 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher sybr select master mix
    VCIP 5′UTR does not contain cryptic promoter activity or evidence of spurious splicing. (A) <t>U2OS</t> cells were transfected with the β-Gal/EMPTY/CAT, β-Gal/VCIP/CAT, or HP-β-Gal/VCIP/CAT bicistronic plasmid. β-Gal and CAT activity was measured from cell lysates taken 24 h posttransfection and normalized to the number of transfected cells based on neomycin activity. Reported β-Gal and CAT activities are expressed relative to measurements obtained for β-Gal/EMPTY/CAT. Bars represent the averages ± SEM from three independent experiments. (B) Quantitative RT-PCR was performed with total <t>RNA</t> isolated from U2OS cells transfected with the β-Gal/EMCV/CAT, β-Gal/EMPTY/CAT, or β-Gal/VCIP/CAT bicistronic plasmid. Q-PCRs were carried out to quantify the number of β-Gal and CAT cistrons expressed in each plasmid. The CAT/β-Gal ratio was calculated as 2 −[Ct(CAT) − Ct(β-Gal)] , where Ct is the crossing point. The bars represent the means ± SEM from six to seven independent experiments performed in triplicate.
    Sybr Select Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high capacity cdna reverse transcription kit
    VCIP 5′UTR does not contain cryptic promoter activity or evidence of spurious splicing. (A) <t>U2OS</t> cells were transfected with the β-Gal/EMPTY/CAT, β-Gal/VCIP/CAT, or HP-β-Gal/VCIP/CAT bicistronic plasmid. β-Gal and CAT activity was measured from cell lysates taken 24 h posttransfection and normalized to the number of transfected cells based on neomycin activity. Reported β-Gal and CAT activities are expressed relative to measurements obtained for β-Gal/EMPTY/CAT. Bars represent the averages ± SEM from three independent experiments. (B) Quantitative RT-PCR was performed with total <t>RNA</t> isolated from U2OS cells transfected with the β-Gal/EMCV/CAT, β-Gal/EMPTY/CAT, or β-Gal/VCIP/CAT bicistronic plasmid. Q-PCRs were carried out to quantify the number of β-Gal and CAT cistrons expressed in each plasmid. The CAT/β-Gal ratio was calculated as 2 −[Ct(CAT) − Ct(β-Gal)] , where Ct is the crossing point. The bars represent the means ± SEM from six to seven independent experiments performed in triplicate.
    High Capacity Cdna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 117891 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa primescript rt reagent kit
    VCIP 5′UTR does not contain cryptic promoter activity or evidence of spurious splicing. (A) <t>U2OS</t> cells were transfected with the β-Gal/EMPTY/CAT, β-Gal/VCIP/CAT, or HP-β-Gal/VCIP/CAT bicistronic plasmid. β-Gal and CAT activity was measured from cell lysates taken 24 h posttransfection and normalized to the number of transfected cells based on neomycin activity. Reported β-Gal and CAT activities are expressed relative to measurements obtained for β-Gal/EMPTY/CAT. Bars represent the averages ± SEM from three independent experiments. (B) Quantitative RT-PCR was performed with total <t>RNA</t> isolated from U2OS cells transfected with the β-Gal/EMCV/CAT, β-Gal/EMPTY/CAT, or β-Gal/VCIP/CAT bicistronic plasmid. Q-PCRs were carried out to quantify the number of β-Gal and CAT cistrons expressed in each plasmid. The CAT/β-Gal ratio was calculated as 2 −[Ct(CAT) − Ct(β-Gal)] , where Ct is the crossing point. The bars represent the means ± SEM from six to seven independent experiments performed in triplicate.
    Primescript Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 72258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher superscript ii reverse transcriptase
    VCIP 5′UTR does not contain cryptic promoter activity or evidence of spurious splicing. (A) <t>U2OS</t> cells were transfected with the β-Gal/EMPTY/CAT, β-Gal/VCIP/CAT, or HP-β-Gal/VCIP/CAT bicistronic plasmid. β-Gal and CAT activity was measured from cell lysates taken 24 h posttransfection and normalized to the number of transfected cells based on neomycin activity. Reported β-Gal and CAT activities are expressed relative to measurements obtained for β-Gal/EMPTY/CAT. Bars represent the averages ± SEM from three independent experiments. (B) Quantitative RT-PCR was performed with total <t>RNA</t> isolated from U2OS cells transfected with the β-Gal/EMCV/CAT, β-Gal/EMPTY/CAT, or β-Gal/VCIP/CAT bicistronic plasmid. Q-PCRs were carried out to quantify the number of β-Gal and CAT cistrons expressed in each plasmid. The CAT/β-Gal ratio was calculated as 2 −[Ct(CAT) − Ct(β-Gal)] , where Ct is the crossing point. The bars represent the means ± SEM from six to seven independent experiments performed in triplicate.
    Superscript Ii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 90426 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bio-Rad thermocycler
    VCIP 5′UTR does not contain cryptic promoter activity or evidence of spurious splicing. (A) <t>U2OS</t> cells were transfected with the β-Gal/EMPTY/CAT, β-Gal/VCIP/CAT, or HP-β-Gal/VCIP/CAT bicistronic plasmid. β-Gal and CAT activity was measured from cell lysates taken 24 h posttransfection and normalized to the number of transfected cells based on neomycin activity. Reported β-Gal and CAT activities are expressed relative to measurements obtained for β-Gal/EMPTY/CAT. Bars represent the averages ± SEM from three independent experiments. (B) Quantitative RT-PCR was performed with total <t>RNA</t> isolated from U2OS cells transfected with the β-Gal/EMCV/CAT, β-Gal/EMPTY/CAT, or β-Gal/VCIP/CAT bicistronic plasmid. Q-PCRs were carried out to quantify the number of β-Gal and CAT cistrons expressed in each plasmid. The CAT/β-Gal ratio was calculated as 2 −[Ct(CAT) − Ct(β-Gal)] , where Ct is the crossing point. The bars represent the means ± SEM from six to seven independent experiments performed in triplicate.
    Thermocycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 6560 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher geneamp pcr system 9700 thermocycler
    VCIP 5′UTR does not contain cryptic promoter activity or evidence of spurious splicing. (A) <t>U2OS</t> cells were transfected with the β-Gal/EMPTY/CAT, β-Gal/VCIP/CAT, or HP-β-Gal/VCIP/CAT bicistronic plasmid. β-Gal and CAT activity was measured from cell lysates taken 24 h posttransfection and normalized to the number of transfected cells based on neomycin activity. Reported β-Gal and CAT activities are expressed relative to measurements obtained for β-Gal/EMPTY/CAT. Bars represent the averages ± SEM from three independent experiments. (B) Quantitative RT-PCR was performed with total <t>RNA</t> isolated from U2OS cells transfected with the β-Gal/EMCV/CAT, β-Gal/EMPTY/CAT, or β-Gal/VCIP/CAT bicistronic plasmid. Q-PCRs were carried out to quantify the number of β-Gal and CAT cistrons expressed in each plasmid. The CAT/β-Gal ratio was calculated as 2 −[Ct(CAT) − Ct(β-Gal)] , where Ct is the crossing point. The bars represent the means ± SEM from six to seven independent experiments performed in triplicate.
    Geneamp Pcr System 9700 Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1993 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher superscript iii reverse transcriptase
    Developmental profile of apoLTP-II/I (A) and <t>apoLTP-III</t> (B) gene expression. Total <t>RNA</t> was prepared from the fat body of fourth and fifth instar larvae, pupae, and adults. Quantification of transcripts was carried out by qPCR. The y-axis (relative amount)
    Superscript Iii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 90401 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum taq polymerase
    Developmental profile of apoLTP-II/I (A) and <t>apoLTP-III</t> (B) gene expression. Total <t>RNA</t> was prepared from the fat body of fourth and fifth instar larvae, pupae, and adults. Quantification of transcripts was carried out by qPCR. The y-axis (relative amount)
    Platinum Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8048 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega m mlv reverse transcriptase
    Developmental profile of apoLTP-II/I (A) and <t>apoLTP-III</t> (B) gene expression. Total <t>RNA</t> was prepared from the fat body of fourth and fifth instar larvae, pupae, and adults. Quantification of transcripts was carried out by qPCR. The y-axis (relative amount)
    M Mlv Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 33849 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rneasy mini kit
    Developmental profile of apoLTP-II/I (A) and <t>apoLTP-III</t> (B) gene expression. Total <t>RNA</t> was prepared from the fat body of fourth and fifth instar larvae, pupae, and adults. Quantification of transcripts was carried out by qPCR. The y-axis (relative amount)
    Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 343576 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher taq dna polymerase
    Developmental profile of apoLTP-II/I (A) and <t>apoLTP-III</t> (B) gene expression. Total <t>RNA</t> was prepared from the fat body of fourth and fifth instar larvae, pupae, and adults. Quantification of transcripts was carried out by qPCR. The y-axis (relative amount)
    Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 44901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad iscript cdna synthesis kit
    Developmental profile of apoLTP-II/I (A) and <t>apoLTP-III</t> (B) gene expression. Total <t>RNA</t> was prepared from the fat body of fourth and fifth instar larvae, pupae, and adults. Quantification of transcripts was carried out by qPCR. The y-axis (relative amount)
    Iscript Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 95548 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    iscript cdna synthesis kit - by Bioz Stars, 2020-09
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    99
    Thermo Fisher pcr buffer
    Developmental profile of apoLTP-II/I (A) and <t>apoLTP-III</t> (B) gene expression. Total <t>RNA</t> was prepared from the fat body of fourth and fifth instar larvae, pupae, and adults. Quantification of transcripts was carried out by qPCR. The y-axis (relative amount)
    Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 28828 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad real time pcr
    Developmental profile of apoLTP-II/I (A) and <t>apoLTP-III</t> (B) gene expression. Total <t>RNA</t> was prepared from the fat body of fourth and fifth instar larvae, pupae, and adults. Quantification of transcripts was carried out by qPCR. The y-axis (relative amount)
    Real Time Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 12747 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher dntps
    Developmental profile of apoLTP-II/I (A) and <t>apoLTP-III</t> (B) gene expression. Total <t>RNA</t> was prepared from the fat body of fourth and fifth instar larvae, pupae, and adults. Quantification of transcripts was carried out by qPCR. The y-axis (relative amount)
    Dntps, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 52680 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    VCIP 5′UTR does not contain cryptic promoter activity or evidence of spurious splicing. (A) U2OS cells were transfected with the β-Gal/EMPTY/CAT, β-Gal/VCIP/CAT, or HP-β-Gal/VCIP/CAT bicistronic plasmid. β-Gal and CAT activity was measured from cell lysates taken 24 h posttransfection and normalized to the number of transfected cells based on neomycin activity. Reported β-Gal and CAT activities are expressed relative to measurements obtained for β-Gal/EMPTY/CAT. Bars represent the averages ± SEM from three independent experiments. (B) Quantitative RT-PCR was performed with total RNA isolated from U2OS cells transfected with the β-Gal/EMCV/CAT, β-Gal/EMPTY/CAT, or β-Gal/VCIP/CAT bicistronic plasmid. Q-PCRs were carried out to quantify the number of β-Gal and CAT cistrons expressed in each plasmid. The CAT/β-Gal ratio was calculated as 2 −[Ct(CAT) − Ct(β-Gal)] , where Ct is the crossing point. The bars represent the means ± SEM from six to seven independent experiments performed in triplicate.

    Journal: Molecular and Cellular Biology

    Article Title: Perk-Dependent Translational Regulation Promotes Tumor Cell Adaptation and Angiogenesis in Response to Hypoxic Stress ▿Perk-Dependent Translational Regulation Promotes Tumor Cell Adaptation and Angiogenesis in Response to Hypoxic Stress ▿ †

    doi: 10.1128/MCB.01145-06

    Figure Lengend Snippet: VCIP 5′UTR does not contain cryptic promoter activity or evidence of spurious splicing. (A) U2OS cells were transfected with the β-Gal/EMPTY/CAT, β-Gal/VCIP/CAT, or HP-β-Gal/VCIP/CAT bicistronic plasmid. β-Gal and CAT activity was measured from cell lysates taken 24 h posttransfection and normalized to the number of transfected cells based on neomycin activity. Reported β-Gal and CAT activities are expressed relative to measurements obtained for β-Gal/EMPTY/CAT. Bars represent the averages ± SEM from three independent experiments. (B) Quantitative RT-PCR was performed with total RNA isolated from U2OS cells transfected with the β-Gal/EMCV/CAT, β-Gal/EMPTY/CAT, or β-Gal/VCIP/CAT bicistronic plasmid. Q-PCRs were carried out to quantify the number of β-Gal and CAT cistrons expressed in each plasmid. The CAT/β-Gal ratio was calculated as 2 −[Ct(CAT) − Ct(β-Gal)] , where Ct is the crossing point. The bars represent the means ± SEM from six to seven independent experiments performed in triplicate.

    Article Snippet: Total RNA was isolated from U2OS cells transfected with the β-Gal/EMCV/CAT, β-Gal/EMPTY/CAT, or β-Gal/VCIP/CAT reporter plasmid and reverse transcribed using SuperScript II (Invitrogen).

    Techniques: Activity Assay, Transfection, Plasmid Preparation, Quantitative RT-PCR, Isolation

    Developmental profile of apoLTP-II/I (A) and apoLTP-III (B) gene expression. Total RNA was prepared from the fat body of fourth and fifth instar larvae, pupae, and adults. Quantification of transcripts was carried out by qPCR. The y-axis (relative amount)

    Journal: Journal of Lipid Research

    Article Title: Lipid transfer particle from the silkworm, Bombyx mori, is a novel member of the apoB/large lipid transfer protein family [S]

    doi: 10.1194/jlr.M037093

    Figure Lengend Snippet: Developmental profile of apoLTP-II/I (A) and apoLTP-III (B) gene expression. Total RNA was prepared from the fat body of fourth and fifth instar larvae, pupae, and adults. Quantification of transcripts was carried out by qPCR. The y-axis (relative amount)

    Article Snippet: Single-stranded cDNAs were synthesized from total RNA using Superscript III reverse transcriptase (Invitrogen) and an oligo-dT primer, and then treated with RNaseH (Takara, Otsu, Japan).

    Techniques: Expressing, Real-time Polymerase Chain Reaction