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  • 94
    Bio-Rad thermocycler
    Thermocycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 6560 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Bio-Rad
    Average 94 stars, based on 6560 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    94/100 stars
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    92
    Biometra thermocycler
    Construction of DNA control plasmid designed for the 4 CDC Category A DNA agents (Smallpox virus [seq1], Bacillus anthracis [seq2], Francisella tularensis [seq3], and Yersinia pestis [seq4]). Assembling of the smallpox virus and B. anthracis sequences is presented as an example. Successive steps are indicated by framed numbers. 1, PCR amplification of the two matrix sequences by primers consisting of the stabilization and the restriction site sequences (italics). PCR reactions were carried out in a volume of 50 μl that included 10 mM Tris-HCl [pH 9.0], 1.5 mM MgCl2, 50 mM KCl, 0.1% Triton X-100, 200 μM each dNTP, 0.4 μM of each oligonucleotide primer, 0.4 μM of the single stranded DNA, and 1.5 U of Taq DNA polymerase (Invitrogen, Cergy-Pontoise, France). The <t>thermocycler</t> (Biometra, Göttingen, Germany) profile was 5 min at 95°C, followed by 35 cycles of 30 sec at 95°C, 30 sec at 55°C, and 1 min at 72°C, and terminated by a final extension for 7 min at 72°C. PCR products were electrophorezed in 3% TAE-agarose gel containing ethidium bromide and visualized under UV transillumination. Column purification of the PCR products. PCR products of the expected size were column-purified by using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions, and eluted in 50 μl of RNase free distillated water. When two bands or more were observed by gel analysis, the band of expected size was excised from the gel and purified by glass milk extraction with the GenClean III Kit (Q-Bio-Gene, Carlsbad CA, USA). 2, assemblage was conducted by pair, seq1 with seq2 (resulting in seq1-2). Equal volumes (10 μl) of purified seq1- and seq2-dsDNA were incubated at 37°C in the presence of Sac I . Sac I site is located at the 3' and 5' ends of seq1 and seq2, respectively. 3, the reaction product was column purified using the protocol aforementioned to discard the 15-nt DNA fragments corresponding to the 5' and 3' ends to avoid their re-ligation to their respective complementary sequences at step 5. 4, Overnight incubation at 4°C in the presence of T4 DNA ligase. Ten μl of the reaction was incubated with T4 DNA ligase (Roche, Basel, Switzerland) according to the manufaturer's instructions. 5, PCR amplification by using the external primers (italics) was performed according to the protocol described at step 1. Then column purification using the protocol detailed at step 2 of the resulting PCR product. At this step the seq1-2 PCR product may be cloned into PGEM-T for storage. The same procedure was performed for seq3 and seq4. Ultimately, seq1-2 and seq3-4 were assembled by using the same protocol (sections 1–9). The final product cloned into PGEM-T plasmid includes seq1-2-3-4 flanked by the two Sseq and restriction sites.
    Thermocycler, supplied by Biometra, used in various techniques. Bioz Stars score: 92/100, based on 2278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Biometra
    Average 92 stars, based on 2278 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    92/100 stars
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    93
    Boeckel + Co thermocycler
    Construction of DNA control plasmid designed for the 4 CDC Category A DNA agents (Smallpox virus [seq1], Bacillus anthracis [seq2], Francisella tularensis [seq3], and Yersinia pestis [seq4]). Assembling of the smallpox virus and B. anthracis sequences is presented as an example. Successive steps are indicated by framed numbers. 1, PCR amplification of the two matrix sequences by primers consisting of the stabilization and the restriction site sequences (italics). PCR reactions were carried out in a volume of 50 μl that included 10 mM Tris-HCl [pH 9.0], 1.5 mM MgCl2, 50 mM KCl, 0.1% Triton X-100, 200 μM each dNTP, 0.4 μM of each oligonucleotide primer, 0.4 μM of the single stranded DNA, and 1.5 U of Taq DNA polymerase (Invitrogen, Cergy-Pontoise, France). The <t>thermocycler</t> (Biometra, Göttingen, Germany) profile was 5 min at 95°C, followed by 35 cycles of 30 sec at 95°C, 30 sec at 55°C, and 1 min at 72°C, and terminated by a final extension for 7 min at 72°C. PCR products were electrophorezed in 3% TAE-agarose gel containing ethidium bromide and visualized under UV transillumination. Column purification of the PCR products. PCR products of the expected size were column-purified by using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions, and eluted in 50 μl of RNase free distillated water. When two bands or more were observed by gel analysis, the band of expected size was excised from the gel and purified by glass milk extraction with the GenClean III Kit (Q-Bio-Gene, Carlsbad CA, USA). 2, assemblage was conducted by pair, seq1 with seq2 (resulting in seq1-2). Equal volumes (10 μl) of purified seq1- and seq2-dsDNA were incubated at 37°C in the presence of Sac I . Sac I site is located at the 3' and 5' ends of seq1 and seq2, respectively. 3, the reaction product was column purified using the protocol aforementioned to discard the 15-nt DNA fragments corresponding to the 5' and 3' ends to avoid their re-ligation to their respective complementary sequences at step 5. 4, Overnight incubation at 4°C in the presence of T4 DNA ligase. Ten μl of the reaction was incubated with T4 DNA ligase (Roche, Basel, Switzerland) according to the manufaturer's instructions. 5, PCR amplification by using the external primers (italics) was performed according to the protocol described at step 1. Then column purification using the protocol detailed at step 2 of the resulting PCR product. At this step the seq1-2 PCR product may be cloned into PGEM-T for storage. The same procedure was performed for seq3 and seq4. Ultimately, seq1-2 and seq3-4 were assembled by using the same protocol (sections 1–9). The final product cloned into PGEM-T plasmid includes seq1-2-3-4 flanked by the two Sseq and restriction sites.
    Thermocycler, supplied by Boeckel + Co, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Boeckel + Co
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    93/100 stars
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    94
    Eppendorf AG thermocycler
    Sensitivity assessment of the LAMP assay for Loa loa using serial dilutions of genomic DNA by the addition of SYBR Green I or by visualization on agarose gel stained with ethidium bromide. (A) Sensitivity assessment performed with a <t>thermocycler.</t> (B) Sensitivity assessment performed with a heating block. Lane Loa: genomic DNA from Loa loa (5 ng); lanes 10 −1 –10 −13 : 10-fold serially dilutions; lanes N, N1, N2, N3: negative controls (no DNA template). Lane M: 50 bp DNA ladder (Molecular weight marker XIII, Roche).
    Thermocycler, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 94/100, based on 4419 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Eppendorf AG
    Average 94 stars, based on 4419 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    94/100 stars
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    93
    Esco Technologies thermocycler
    Sensitivity assessment of the LAMP assay for Loa loa using serial dilutions of genomic DNA by the addition of SYBR Green I or by visualization on agarose gel stained with ethidium bromide. (A) Sensitivity assessment performed with a <t>thermocycler.</t> (B) Sensitivity assessment performed with a heating block. Lane Loa: genomic DNA from Loa loa (5 ng); lanes 10 −1 –10 −13 : 10-fold serially dilutions; lanes N, N1, N2, N3: negative controls (no DNA template). Lane M: 50 bp DNA ladder (Molecular weight marker XIII, Roche).
    Thermocycler, supplied by Esco Technologies, used in various techniques. Bioz Stars score: 93/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Esco Technologies
    Average 93 stars, based on 102 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    93/100 stars
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    93
    MJ Research thermocycler
    Optimization of temperature and paper type. (a) Temperature optimization among 65 °C, 68 °C and 70 °C using a conventional <t>thermocycler,</t> as confirmed with 3% w/v agarose gel electrophoresis. (b) Paper type optimization among NC (nitrocellulose), G4 (cellulose grade 4) and G1 (cellulose grade 1) papers, as confirmed with 3% w/v agarose gel electrophoresis.
    Thermocycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 93/100, based on 2253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/MJ Research
    Average 93 stars, based on 2253 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    93/100 stars
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    92
    Opticon Inc thermocycler
    Optimization of temperature and paper type. (a) Temperature optimization among 65 °C, 68 °C and 70 °C using a conventional <t>thermocycler,</t> as confirmed with 3% w/v agarose gel electrophoresis. (b) Paper type optimization among NC (nitrocellulose), G4 (cellulose grade 4) and G1 (cellulose grade 1) papers, as confirmed with 3% w/v agarose gel electrophoresis.
    Thermocycler, supplied by Opticon Inc, used in various techniques. Bioz Stars score: 92/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Opticon Inc
    Average 92 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    92/100 stars
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    92
    PEQLAB thermocycler
    Optimization of temperature and paper type. (a) Temperature optimization among 65 °C, 68 °C and 70 °C using a conventional <t>thermocycler,</t> as confirmed with 3% w/v agarose gel electrophoresis. (b) Paper type optimization among NC (nitrocellulose), G4 (cellulose grade 4) and G1 (cellulose grade 1) papers, as confirmed with 3% w/v agarose gel electrophoresis.
    Thermocycler, supplied by PEQLAB, used in various techniques. Bioz Stars score: 92/100, based on 220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/PEQLAB
    Average 92 stars, based on 220 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    92/100 stars
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    92
    PerkinElmer thermocycler
    Optimization of temperature and paper type. (a) Temperature optimization among 65 °C, 68 °C and 70 °C using a conventional <t>thermocycler,</t> as confirmed with 3% w/v agarose gel electrophoresis. (b) Paper type optimization among NC (nitrocellulose), G4 (cellulose grade 4) and G1 (cellulose grade 1) papers, as confirmed with 3% w/v agarose gel electrophoresis.
    Thermocycler, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 2039 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/PerkinElmer
    Average 92 stars, based on 2039 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    92/100 stars
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    92
    Quanta Biosciences thermocycler
    Optimization of temperature and paper type. (a) Temperature optimization among 65 °C, 68 °C and 70 °C using a conventional <t>thermocycler,</t> as confirmed with 3% w/v agarose gel electrophoresis. (b) Paper type optimization among NC (nitrocellulose), G4 (cellulose grade 4) and G1 (cellulose grade 1) papers, as confirmed with 3% w/v agarose gel electrophoresis.
    Thermocycler, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Quanta Biosciences
    Average 92 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    92/100 stars
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    92
    Roche thermocycler
    Optimization of temperature and paper type. (a) Temperature optimization among 65 °C, 68 °C and 70 °C using a conventional <t>thermocycler,</t> as confirmed with 3% w/v agarose gel electrophoresis. (b) Paper type optimization among NC (nitrocellulose), G4 (cellulose grade 4) and G1 (cellulose grade 1) papers, as confirmed with 3% w/v agarose gel electrophoresis.
    Thermocycler, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 700 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Roche
    Average 92 stars, based on 700 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    92/100 stars
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    93
    Stratagene thermocycler
    Optimization of temperature and paper type. (a) Temperature optimization among 65 °C, 68 °C and 70 °C using a conventional <t>thermocycler,</t> as confirmed with 3% w/v agarose gel electrophoresis. (b) Paper type optimization among NC (nitrocellulose), G4 (cellulose grade 4) and G1 (cellulose grade 1) papers, as confirmed with 3% w/v agarose gel electrophoresis.
    Thermocycler, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 450 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Stratagene
    Average 93 stars, based on 450 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    93/100 stars
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    94
    Thermo Fisher thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 5940 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Thermo Fisher
    Average 94 stars, based on 5940 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    94/100 stars
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    92
    Astec thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermocycler, supplied by Astec, used in various techniques. Bioz Stars score: 92/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Astec
    Average 92 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    92/100 stars
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    92
    BioMed Diagnostics Inc thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermocycler, supplied by BioMed Diagnostics Inc, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/BioMed Diagnostics Inc
    Average 92 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    92/100 stars
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    92
    Bioneer Corporation thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermocycler, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 92/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Bioneer Corporation
    Average 92 stars, based on 80 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    92/100 stars
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    92
    Labnet thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermocycler, supplied by Labnet, used in various techniques. Bioz Stars score: 92/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Labnet
    Average 92 stars, based on 79 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    92/100 stars
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    92
    MWG-Biotech thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermocycler, supplied by MWG-Biotech, used in various techniques. Bioz Stars score: 92/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/MWG-Biotech
    Average 92 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    92/100 stars
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    92
    New Brunswick Scientific thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermocycler, supplied by New Brunswick Scientific, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/New Brunswick Scientific
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    92/100 stars
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    92
    Agilent technologies thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermocycler, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Agilent technologies
    Average 92 stars, based on 308 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    92/100 stars
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    90
    Fisher Scientific thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermocycler, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Fisher Scientific
    Average 90 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-08
    90/100 stars
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    Image Search Results


    Construction of DNA control plasmid designed for the 4 CDC Category A DNA agents (Smallpox virus [seq1], Bacillus anthracis [seq2], Francisella tularensis [seq3], and Yersinia pestis [seq4]). Assembling of the smallpox virus and B. anthracis sequences is presented as an example. Successive steps are indicated by framed numbers. 1, PCR amplification of the two matrix sequences by primers consisting of the stabilization and the restriction site sequences (italics). PCR reactions were carried out in a volume of 50 μl that included 10 mM Tris-HCl [pH 9.0], 1.5 mM MgCl2, 50 mM KCl, 0.1% Triton X-100, 200 μM each dNTP, 0.4 μM of each oligonucleotide primer, 0.4 μM of the single stranded DNA, and 1.5 U of Taq DNA polymerase (Invitrogen, Cergy-Pontoise, France). The thermocycler (Biometra, Göttingen, Germany) profile was 5 min at 95°C, followed by 35 cycles of 30 sec at 95°C, 30 sec at 55°C, and 1 min at 72°C, and terminated by a final extension for 7 min at 72°C. PCR products were electrophorezed in 3% TAE-agarose gel containing ethidium bromide and visualized under UV transillumination. Column purification of the PCR products. PCR products of the expected size were column-purified by using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions, and eluted in 50 μl of RNase free distillated water. When two bands or more were observed by gel analysis, the band of expected size was excised from the gel and purified by glass milk extraction with the GenClean III Kit (Q-Bio-Gene, Carlsbad CA, USA). 2, assemblage was conducted by pair, seq1 with seq2 (resulting in seq1-2). Equal volumes (10 μl) of purified seq1- and seq2-dsDNA were incubated at 37°C in the presence of Sac I . Sac I site is located at the 3' and 5' ends of seq1 and seq2, respectively. 3, the reaction product was column purified using the protocol aforementioned to discard the 15-nt DNA fragments corresponding to the 5' and 3' ends to avoid their re-ligation to their respective complementary sequences at step 5. 4, Overnight incubation at 4°C in the presence of T4 DNA ligase. Ten μl of the reaction was incubated with T4 DNA ligase (Roche, Basel, Switzerland) according to the manufaturer's instructions. 5, PCR amplification by using the external primers (italics) was performed according to the protocol described at step 1. Then column purification using the protocol detailed at step 2 of the resulting PCR product. At this step the seq1-2 PCR product may be cloned into PGEM-T for storage. The same procedure was performed for seq3 and seq4. Ultimately, seq1-2 and seq3-4 were assembled by using the same protocol (sections 1–9). The final product cloned into PGEM-T plasmid includes seq1-2-3-4 flanked by the two Sseq and restriction sites.

    Journal: BMC Microbiology

    Article Title: Multi-pathogens sequence containing plasmids as positive controls for universal detection of potential agents of bioterrorism

    doi: 10.1186/1471-2180-4-21

    Figure Lengend Snippet: Construction of DNA control plasmid designed for the 4 CDC Category A DNA agents (Smallpox virus [seq1], Bacillus anthracis [seq2], Francisella tularensis [seq3], and Yersinia pestis [seq4]). Assembling of the smallpox virus and B. anthracis sequences is presented as an example. Successive steps are indicated by framed numbers. 1, PCR amplification of the two matrix sequences by primers consisting of the stabilization and the restriction site sequences (italics). PCR reactions were carried out in a volume of 50 μl that included 10 mM Tris-HCl [pH 9.0], 1.5 mM MgCl2, 50 mM KCl, 0.1% Triton X-100, 200 μM each dNTP, 0.4 μM of each oligonucleotide primer, 0.4 μM of the single stranded DNA, and 1.5 U of Taq DNA polymerase (Invitrogen, Cergy-Pontoise, France). The thermocycler (Biometra, Göttingen, Germany) profile was 5 min at 95°C, followed by 35 cycles of 30 sec at 95°C, 30 sec at 55°C, and 1 min at 72°C, and terminated by a final extension for 7 min at 72°C. PCR products were electrophorezed in 3% TAE-agarose gel containing ethidium bromide and visualized under UV transillumination. Column purification of the PCR products. PCR products of the expected size were column-purified by using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions, and eluted in 50 μl of RNase free distillated water. When two bands or more were observed by gel analysis, the band of expected size was excised from the gel and purified by glass milk extraction with the GenClean III Kit (Q-Bio-Gene, Carlsbad CA, USA). 2, assemblage was conducted by pair, seq1 with seq2 (resulting in seq1-2). Equal volumes (10 μl) of purified seq1- and seq2-dsDNA were incubated at 37°C in the presence of Sac I . Sac I site is located at the 3' and 5' ends of seq1 and seq2, respectively. 3, the reaction product was column purified using the protocol aforementioned to discard the 15-nt DNA fragments corresponding to the 5' and 3' ends to avoid their re-ligation to their respective complementary sequences at step 5. 4, Overnight incubation at 4°C in the presence of T4 DNA ligase. Ten μl of the reaction was incubated with T4 DNA ligase (Roche, Basel, Switzerland) according to the manufaturer's instructions. 5, PCR amplification by using the external primers (italics) was performed according to the protocol described at step 1. Then column purification using the protocol detailed at step 2 of the resulting PCR product. At this step the seq1-2 PCR product may be cloned into PGEM-T for storage. The same procedure was performed for seq3 and seq4. Ultimately, seq1-2 and seq3-4 were assembled by using the same protocol (sections 1–9). The final product cloned into PGEM-T plasmid includes seq1-2-3-4 flanked by the two Sseq and restriction sites.

    Article Snippet: The thermocycler (Biometra, Göttingen, Germany) profile was 5 min at 95°C, followed by 35 cycles of 30 sec at 95°C, 30 sec at 55°C, and 1 min at 72°C, and terminated by a final extension for 7 min at 72°C.

    Techniques: Plasmid Preparation, Polymerase Chain Reaction, Amplification, Size-exclusion Chromatography, Agarose Gel Electrophoresis, Purification, Incubation, Ligation, Clone Assay

    Sensitivity assessment of the LAMP assay for Loa loa using serial dilutions of genomic DNA by the addition of SYBR Green I or by visualization on agarose gel stained with ethidium bromide. (A) Sensitivity assessment performed with a thermocycler. (B) Sensitivity assessment performed with a heating block. Lane Loa: genomic DNA from Loa loa (5 ng); lanes 10 −1 –10 −13 : 10-fold serially dilutions; lanes N, N1, N2, N3: negative controls (no DNA template). Lane M: 50 bp DNA ladder (Molecular weight marker XIII, Roche).

    Journal: PLoS ONE

    Article Title: Development of a Highly Sensitive Loop-Mediated Isothermal Amplification (LAMP) Method for the Detection of Loa loa

    doi: 10.1371/journal.pone.0094664

    Figure Lengend Snippet: Sensitivity assessment of the LAMP assay for Loa loa using serial dilutions of genomic DNA by the addition of SYBR Green I or by visualization on agarose gel stained with ethidium bromide. (A) Sensitivity assessment performed with a thermocycler. (B) Sensitivity assessment performed with a heating block. Lane Loa: genomic DNA from Loa loa (5 ng); lanes 10 −1 –10 −13 : 10-fold serially dilutions; lanes N, N1, N2, N3: negative controls (no DNA template). Lane M: 50 bp DNA ladder (Molecular weight marker XIII, Roche).

    Article Snippet: To establish the standard protocol for the LAMP method, the reaction mixture was incubated in a conventional heating block (K Dry-Bath) or in a thermocycler (Mastercycler Gradient-96well; Eppendorf) at a range of temperatures (61, 63 and 65°C) for 60 min to optimize the reaction conditions and then heated at 80°C for 5 min to terminate the reaction.

    Techniques: Lamp Assay, SYBR Green Assay, Agarose Gel Electrophoresis, Staining, Blocking Assay, Molecular Weight, Marker

    Optimization of temperature and paper type. (a) Temperature optimization among 65 °C, 68 °C and 70 °C using a conventional thermocycler, as confirmed with 3% w/v agarose gel electrophoresis. (b) Paper type optimization among NC (nitrocellulose), G4 (cellulose grade 4) and G1 (cellulose grade 1) papers, as confirmed with 3% w/v agarose gel electrophoresis.

    Journal: Scientific Reports

    Article Title: Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips

    doi: 10.1038/s41598-018-30797-9

    Figure Lengend Snippet: Optimization of temperature and paper type. (a) Temperature optimization among 65 °C, 68 °C and 70 °C using a conventional thermocycler, as confirmed with 3% w/v agarose gel electrophoresis. (b) Paper type optimization among NC (nitrocellulose), G4 (cellulose grade 4) and G1 (cellulose grade 1) papers, as confirmed with 3% w/v agarose gel electrophoresis.

    Article Snippet: Temperature optimization The optimum temperature was evaluated by conventionally amplifying the NTC and target at three different temperatures, 65 °C, 68 °C, and 70 °C for 30 minutes using a thermocycler (MJ Research, Waltham, MA, USA), followed by refrigeration at 4 °C in preparation for gel electrophoresis confirmation.

    Techniques: Agarose Gel Electrophoresis

    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same thermocycler and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.

    Journal: Scientific Reports

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest

    doi: 10.1038/s41598-017-18216-x

    Figure Lengend Snippet: Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same thermocycler and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.

    Article Snippet: The PCR assays were performed in a thermocycler (Veriti 96 wells, Applied Biosystems) with the following cycling parameters: an initial denaturation at 94 °C for 2 min followed by 40 cycles of denaturation at 94 °C for 30 sec, annealing at 54 °C for 20 sec and extension at 72 °C for 30 sec followed by a final extension incubation at 72 °C for 2 min.

    Techniques: Nested PCR, Polymerase Chain Reaction, Negative Control

    Differential diagnosis of Plasmodium simium infection by nested/PCR followed by a digestion with Hpy CH4III restriction enzyme of 9 infected human samples from Atlantic Forest in Rio de Janeiro/RJ (H2 – H9) and one from Amazon endemic region (H1) according to Additional file 1. 3% agarose gel stained with ethidium bromide. MM:1 kb Plus Ladder (ThermoFischer, Calrsbad, CA, USA). Reactions were performed simultaneously in the same thermocycler and splited in different gels.D: Digested; ND: Non Digested. PC Pv: Positive Control for P. vivax , PC Ps: positive control for P. simium . NC: Negative Control (without DNA).

    Journal: Scientific Reports

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest

    doi: 10.1038/s41598-017-18216-x

    Figure Lengend Snippet: Differential diagnosis of Plasmodium simium infection by nested/PCR followed by a digestion with Hpy CH4III restriction enzyme of 9 infected human samples from Atlantic Forest in Rio de Janeiro/RJ (H2 – H9) and one from Amazon endemic region (H1) according to Additional file 1. 3% agarose gel stained with ethidium bromide. MM:1 kb Plus Ladder (ThermoFischer, Calrsbad, CA, USA). Reactions were performed simultaneously in the same thermocycler and splited in different gels.D: Digested; ND: Non Digested. PC Pv: Positive Control for P. vivax , PC Ps: positive control for P. simium . NC: Negative Control (without DNA).

    Article Snippet: The PCR assays were performed in a thermocycler (Veriti 96 wells, Applied Biosystems) with the following cycling parameters: an initial denaturation at 94 °C for 2 min followed by 40 cycles of denaturation at 94 °C for 30 sec, annealing at 54 °C for 20 sec and extension at 72 °C for 30 sec followed by a final extension incubation at 72 °C for 2 min.

    Techniques: Infection, Nested PCR, Agarose Gel Electrophoresis, Staining, Positive Control, Negative Control

    Nested/PCR-RFLP of P. vivax DNA samples. 15 DNA samples from P. vivax infected individuals from different parts of Amazon: Porto Velho/Rondônia State, Brazil (PvPV/RO1 and 2), Guyana (PvGuy), Ariquemes/Rondônia State, Brazil (PvAri/RO), Venezuela (PvVen), French Guiana (PvFrGui), Novo progresso/Pará State, Brazil (PvNP/PA), Rio Pardo/Amazonas State, Brazil (PvRP/AM1, 2 and 3), Humaita/Amazonas State, Brazil (PvHu/Am1, 2, 3 and 4) and unknown city in Amazonas State, Brazil (PvAM) were used for Nested PCR amplification followed by digestion with Hpy CH4III restriction enzyme. 3% Agarose gel stained with ethidium bromide. Name tags above gels indicated the patients according to Additional file 2. Reactions were performed simultaneously in the same thermocycler and splited in different gels. MM: 1 kb Plus Ladder. D: Digested (8 μL of digestion); ND: Non Digested (the equivalent amount of PCR product used in the digestion, 6.5 μL of samples and 5 μL of controls); PC Pv: positive control of P. vivax (pool of samples from infected patient from Amazonia); PC Ps: positive control of P. simium ( Alouatta g. clamitans infected with P. simium ); NC: negative Control.

    Journal: Scientific Reports

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest

    doi: 10.1038/s41598-017-18216-x

    Figure Lengend Snippet: Nested/PCR-RFLP of P. vivax DNA samples. 15 DNA samples from P. vivax infected individuals from different parts of Amazon: Porto Velho/Rondônia State, Brazil (PvPV/RO1 and 2), Guyana (PvGuy), Ariquemes/Rondônia State, Brazil (PvAri/RO), Venezuela (PvVen), French Guiana (PvFrGui), Novo progresso/Pará State, Brazil (PvNP/PA), Rio Pardo/Amazonas State, Brazil (PvRP/AM1, 2 and 3), Humaita/Amazonas State, Brazil (PvHu/Am1, 2, 3 and 4) and unknown city in Amazonas State, Brazil (PvAM) were used for Nested PCR amplification followed by digestion with Hpy CH4III restriction enzyme. 3% Agarose gel stained with ethidium bromide. Name tags above gels indicated the patients according to Additional file 2. Reactions were performed simultaneously in the same thermocycler and splited in different gels. MM: 1 kb Plus Ladder. D: Digested (8 μL of digestion); ND: Non Digested (the equivalent amount of PCR product used in the digestion, 6.5 μL of samples and 5 μL of controls); PC Pv: positive control of P. vivax (pool of samples from infected patient from Amazonia); PC Ps: positive control of P. simium ( Alouatta g. clamitans infected with P. simium ); NC: negative Control.

    Article Snippet: The PCR assays were performed in a thermocycler (Veriti 96 wells, Applied Biosystems) with the following cycling parameters: an initial denaturation at 94 °C for 2 min followed by 40 cycles of denaturation at 94 °C for 30 sec, annealing at 54 °C for 20 sec and extension at 72 °C for 30 sec followed by a final extension incubation at 72 °C for 2 min.

    Techniques: Nested PCR, Infection, Amplification, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction, Positive Control, Negative Control

    Differential Diagnosis of Plasmodium simium infection by nested/PCR followed by a digestion with Hpy CH4III restriction enzyme of 16 non-human primate samples: ( A ) 2 captive NHP from Rio de Janeiro/RJ ( Sapajus xanthosternos 2098; Cacajao melanocephalus 2302), one free-living Alouatta g. clamitans from Rio de Janeiro State (3636) and 6 free-living Alouatta g. clamitans from Joinville/SC, Brazil (J9, J11, J15, J20, J22, J25); ( B ) 7 Alouatta g. clamitans from CEPESBI, Indaial, SC, Brazil (Bl3, Bl6, Bl10, Bl28, Bl61, Bl64, Bl69), *Captive NHPs, all the other were free-living. More details about each sample see Additional file 1. 3% agarose gel stained with ethidium bromide. MM:1 kb Plus Ladder (ThermoFischer). Reactions were performed simultaneously in the same thermocycler and splited in different gels. PC Pv: Positive Control for P. vivax , PC Ps: positive control for P. simium . NC: Negative Control (without DNA).

    Journal: Scientific Reports

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest

    doi: 10.1038/s41598-017-18216-x

    Figure Lengend Snippet: Differential Diagnosis of Plasmodium simium infection by nested/PCR followed by a digestion with Hpy CH4III restriction enzyme of 16 non-human primate samples: ( A ) 2 captive NHP from Rio de Janeiro/RJ ( Sapajus xanthosternos 2098; Cacajao melanocephalus 2302), one free-living Alouatta g. clamitans from Rio de Janeiro State (3636) and 6 free-living Alouatta g. clamitans from Joinville/SC, Brazil (J9, J11, J15, J20, J22, J25); ( B ) 7 Alouatta g. clamitans from CEPESBI, Indaial, SC, Brazil (Bl3, Bl6, Bl10, Bl28, Bl61, Bl64, Bl69), *Captive NHPs, all the other were free-living. More details about each sample see Additional file 1. 3% agarose gel stained with ethidium bromide. MM:1 kb Plus Ladder (ThermoFischer). Reactions were performed simultaneously in the same thermocycler and splited in different gels. PC Pv: Positive Control for P. vivax , PC Ps: positive control for P. simium . NC: Negative Control (without DNA).

    Article Snippet: The PCR assays were performed in a thermocycler (Veriti 96 wells, Applied Biosystems) with the following cycling parameters: an initial denaturation at 94 °C for 2 min followed by 40 cycles of denaturation at 94 °C for 30 sec, annealing at 54 °C for 20 sec and extension at 72 °C for 30 sec followed by a final extension incubation at 72 °C for 2 min.

    Techniques: Infection, Nested PCR, Agarose Gel Electrophoresis, Staining, Positive Control, Negative Control