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  • 99
    Thermo Fisher todd hewitt broth thb
    Growth of S. dysgalactiae subsp. equisimilis in the presence of LL-37 and DrsG. S. dysgalactiae subsp. equisimilis MD985 ( drsG negative) was grown in the presence of LL-37 and/or DrsGL (A), DrsGS (B), or SIC (C). After a 2.5-h incubation, the bacteria were recovered, plated onto <t>Todd-Hewitt</t> agar, and incubated overnight. The percentage of growth compared to that of controls was determined for each individual assay. The results presented are the means for three independent experiments.
    Todd Hewitt Broth Thb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/todd hewitt broth thb/product/Thermo Fisher
    Average 99 stars, based on 138 article reviews
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    todd hewitt broth thb - by Bioz Stars, 2020-04
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    hb5  (ATCC)
    91
    ATCC hb5
    Transfection of <t>CD21</t> into Ramos cells impedes anti-CD19 uptake. Upper panels: freshly isolated human B-cells do not internalize Alexa555-labeled anti-CD19 within 3 h (A, red channel in C), although it does redistribute into patches on the cell surface (arrows) that co-localize with Alexa488-labeled anti-CD21 added post-uptake on ice (B, green channel in C). Scale bar = 20 μm. Lower panels: Ramos cells (left panels) stably expressing a high (clone 1, right panels) or medium (clone 3, middle panels) level of CD21 were incubated with Alexa555-labeled anti-CD19 (G–I and red channel in M–O) and Alexa647-transferrin (J–L and M–O, shown in green channel for better contrast) for 3 h at 37°C, then chilled and incubated with Alexa488-conjugated anti-CD21 antibodies (D–F) on ice prior to fixation and imaging. Anti-CD19 uptake is impeded by increased CD21 expression, while transferrin uptake is unaffected. Internalized anti-CD19 antibodies do not significantly co-localize with the recycling transferrin at this time-point, as seen by lack of yellow colour in the respective overlaid images (M–O). Gamma levels were adjusted where required for clarity. Scale bar = 20 μm.
    Hb5, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hb5/product/ATCC
    Average 91 stars, based on 20 article reviews
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    hb5 - by Bioz Stars, 2020-04
    91/100 stars
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    86
    Difco thb agar
    Transfection of <t>CD21</t> into Ramos cells impedes anti-CD19 uptake. Upper panels: freshly isolated human B-cells do not internalize Alexa555-labeled anti-CD19 within 3 h (A, red channel in C), although it does redistribute into patches on the cell surface (arrows) that co-localize with Alexa488-labeled anti-CD21 added post-uptake on ice (B, green channel in C). Scale bar = 20 μm. Lower panels: Ramos cells (left panels) stably expressing a high (clone 1, right panels) or medium (clone 3, middle panels) level of CD21 were incubated with Alexa555-labeled anti-CD19 (G–I and red channel in M–O) and Alexa647-transferrin (J–L and M–O, shown in green channel for better contrast) for 3 h at 37°C, then chilled and incubated with Alexa488-conjugated anti-CD21 antibodies (D–F) on ice prior to fixation and imaging. Anti-CD19 uptake is impeded by increased CD21 expression, while transferrin uptake is unaffected. Internalized anti-CD19 antibodies do not significantly co-localize with the recycling transferrin at this time-point, as seen by lack of yellow colour in the respective overlaid images (M–O). Gamma levels were adjusted where required for clarity. Scale bar = 20 μm.
    Thb Agar, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thb agar - by Bioz Stars, 2020-04
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    89
    Thermo Fisher pfastbac thb
    Transfection of <t>CD21</t> into Ramos cells impedes anti-CD19 uptake. Upper panels: freshly isolated human B-cells do not internalize Alexa555-labeled anti-CD19 within 3 h (A, red channel in C), although it does redistribute into patches on the cell surface (arrows) that co-localize with Alexa488-labeled anti-CD21 added post-uptake on ice (B, green channel in C). Scale bar = 20 μm. Lower panels: Ramos cells (left panels) stably expressing a high (clone 1, right panels) or medium (clone 3, middle panels) level of CD21 were incubated with Alexa555-labeled anti-CD19 (G–I and red channel in M–O) and Alexa647-transferrin (J–L and M–O, shown in green channel for better contrast) for 3 h at 37°C, then chilled and incubated with Alexa488-conjugated anti-CD21 antibodies (D–F) on ice prior to fixation and imaging. Anti-CD19 uptake is impeded by increased CD21 expression, while transferrin uptake is unaffected. Internalized anti-CD19 antibodies do not significantly co-localize with the recycling transferrin at this time-point, as seen by lack of yellow colour in the respective overlaid images (M–O). Gamma levels were adjusted where required for clarity. Scale bar = 20 μm.
    Pfastbac Thb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Becton Dickinson thb plates
    Transfection of <t>CD21</t> into Ramos cells impedes anti-CD19 uptake. Upper panels: freshly isolated human B-cells do not internalize Alexa555-labeled anti-CD19 within 3 h (A, red channel in C), although it does redistribute into patches on the cell surface (arrows) that co-localize with Alexa488-labeled anti-CD21 added post-uptake on ice (B, green channel in C). Scale bar = 20 μm. Lower panels: Ramos cells (left panels) stably expressing a high (clone 1, right panels) or medium (clone 3, middle panels) level of CD21 were incubated with Alexa555-labeled anti-CD19 (G–I and red channel in M–O) and Alexa647-transferrin (J–L and M–O, shown in green channel for better contrast) for 3 h at 37°C, then chilled and incubated with Alexa488-conjugated anti-CD21 antibodies (D–F) on ice prior to fixation and imaging. Anti-CD19 uptake is impeded by increased CD21 expression, while transferrin uptake is unaffected. Internalized anti-CD19 antibodies do not significantly co-localize with the recycling transferrin at this time-point, as seen by lack of yellow colour in the respective overlaid images (M–O). Gamma levels were adjusted where required for clarity. Scale bar = 20 μm.
    Thb Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Millipore thb percoll
    Transfection of <t>CD21</t> into Ramos cells impedes anti-CD19 uptake. Upper panels: freshly isolated human B-cells do not internalize Alexa555-labeled anti-CD19 within 3 h (A, red channel in C), although it does redistribute into patches on the cell surface (arrows) that co-localize with Alexa488-labeled anti-CD21 added post-uptake on ice (B, green channel in C). Scale bar = 20 μm. Lower panels: Ramos cells (left panels) stably expressing a high (clone 1, right panels) or medium (clone 3, middle panels) level of CD21 were incubated with Alexa555-labeled anti-CD19 (G–I and red channel in M–O) and Alexa647-transferrin (J–L and M–O, shown in green channel for better contrast) for 3 h at 37°C, then chilled and incubated with Alexa488-conjugated anti-CD21 antibodies (D–F) on ice prior to fixation and imaging. Anti-CD19 uptake is impeded by increased CD21 expression, while transferrin uptake is unaffected. Internalized anti-CD19 antibodies do not significantly co-localize with the recycling transferrin at this time-point, as seen by lack of yellow colour in the respective overlaid images (M–O). Gamma levels were adjusted where required for clarity. Scale bar = 20 μm.
    Thb Percoll, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 84 stars, based on 1 article reviews
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    thb percoll - by Bioz Stars, 2020-04
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    85
    Pfizer Inc thb
    Transfection of <t>CD21</t> into Ramos cells impedes anti-CD19 uptake. Upper panels: freshly isolated human B-cells do not internalize Alexa555-labeled anti-CD19 within 3 h (A, red channel in C), although it does redistribute into patches on the cell surface (arrows) that co-localize with Alexa488-labeled anti-CD21 added post-uptake on ice (B, green channel in C). Scale bar = 20 μm. Lower panels: Ramos cells (left panels) stably expressing a high (clone 1, right panels) or medium (clone 3, middle panels) level of CD21 were incubated with Alexa555-labeled anti-CD19 (G–I and red channel in M–O) and Alexa647-transferrin (J–L and M–O, shown in green channel for better contrast) for 3 h at 37°C, then chilled and incubated with Alexa488-conjugated anti-CD21 antibodies (D–F) on ice prior to fixation and imaging. Anti-CD19 uptake is impeded by increased CD21 expression, while transferrin uptake is unaffected. Internalized anti-CD19 antibodies do not significantly co-localize with the recycling transferrin at this time-point, as seen by lack of yellow colour in the respective overlaid images (M–O). Gamma levels were adjusted where required for clarity. Scale bar = 20 μm.
    Thb, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thb/product/Pfizer Inc
    Average 85 stars, based on 5 article reviews
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    thb - by Bioz Stars, 2020-04
    85/100 stars
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    85
    Merck & Co thb
    Transfection of <t>CD21</t> into Ramos cells impedes anti-CD19 uptake. Upper panels: freshly isolated human B-cells do not internalize Alexa555-labeled anti-CD19 within 3 h (A, red channel in C), although it does redistribute into patches on the cell surface (arrows) that co-localize with Alexa488-labeled anti-CD21 added post-uptake on ice (B, green channel in C). Scale bar = 20 μm. Lower panels: Ramos cells (left panels) stably expressing a high (clone 1, right panels) or medium (clone 3, middle panels) level of CD21 were incubated with Alexa555-labeled anti-CD19 (G–I and red channel in M–O) and Alexa647-transferrin (J–L and M–O, shown in green channel for better contrast) for 3 h at 37°C, then chilled and incubated with Alexa488-conjugated anti-CD21 antibodies (D–F) on ice prior to fixation and imaging. Anti-CD19 uptake is impeded by increased CD21 expression, while transferrin uptake is unaffected. Internalized anti-CD19 antibodies do not significantly co-localize with the recycling transferrin at this time-point, as seen by lack of yellow colour in the respective overlaid images (M–O). Gamma levels were adjusted where required for clarity. Scale bar = 20 μm.
    Thb, supplied by Merck & Co, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
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    thb - by Bioz Stars, 2020-04
    85/100 stars
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    92
    Millipore thb
    Scanning electron microscopy of biofilm of S. <t>suis</t> grown in <t>THB</t> broth. (A) With 0 MIC (0 μg mL -1 ) concentration of aqueous extracts of Rhizoma Coptidis ; (B) with 1/2 × MIC (50 μg mL -1 ) concentration of aqueous extracts of Rhizoma Coptidis .
    Thb, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thb/product/Millipore
    Average 92 stars, based on 8 article reviews
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    thb - by Bioz Stars, 2020-04
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    92
    Novartis thb
    Scanning electron microscopy of biofilm of S. <t>suis</t> grown in <t>THB</t> broth. (A) With 0 MIC (0 μg mL -1 ) concentration of aqueous extracts of Rhizoma Coptidis ; (B) with 1/2 × MIC (50 μg mL -1 ) concentration of aqueous extracts of Rhizoma Coptidis .
    Thb, supplied by Novartis, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thb/product/Novartis
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    thb - by Bioz Stars, 2020-04
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    85
    Terumo Corp thb
    Scanning electron microscopy of biofilm of S. <t>suis</t> grown in <t>THB</t> broth. (A) With 0 MIC (0 μg mL -1 ) concentration of aqueous extracts of Rhizoma Coptidis ; (B) with 1/2 × MIC (50 μg mL -1 ) concentration of aqueous extracts of Rhizoma Coptidis .
    Thb, supplied by Terumo Corp, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
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    94
    Thermo Fisher thb
    Stability of plasmid pGU2664 in the absence of selection. (A and B) Numbers of Sp r <t>GBS</t> (red) and the total numbers of GBS (black) recovered from growth conditions with (A) and without (B) antibiotic selection, respectively. Cells were diluted 1:1,000 into fresh <t>THB</t> medium (±Sp) and grown for 12 h in 5 successive culture passages, representing approximately 100 generations. The results presented are averages of the results from three independent experiments compared using AUC Student's t test, with bars showing the standard error.
    Thb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 32 article reviews
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    thb - by Bioz Stars, 2020-04
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    94
    Hardy Diagnostics thb
    (A and B) Growth curves for WT <t>GBS</t> and the Δ ltdR mutant in <t>THB</t> (A) and CDM (B) at 37°C. (C to E) Flow cytometry using serial dilutions of a monoclonal antibody (MAb) to the serotype III capsule to determine the presence of capsule in WT GBS (C) and the Δ ltdR mutant (D). A monoclonal antibody to the serotype Ia capsule was used as the isotype control. (F) Hemolysis assay comparing the hemolysis of sheep blood cells by WT GBS and the Δ ltdR mutant. Representative data from 1 of at least 2 independent experiments are shown. (G and H) Scanning electron microscopy images of WT GBS (G) and Δ ltdR mutant (H) strains. (I) Aggregation assay comparing aggregation of WT GBS, the Δ ltdR mutant, and the complemented strain in THB. (J) Clumping assay comparing clumping of the WT GBS, the Δ ltdR mutant, and the complemented strain in THB containing 0.1% fibrinogen. *, P
    Thb, supplied by Hardy Diagnostics, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 14 article reviews
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    86
    Oxyrase Inc thb
    (A and B) Growth curves for WT <t>GBS</t> and the Δ ltdR mutant in <t>THB</t> (A) and CDM (B) at 37°C. (C to E) Flow cytometry using serial dilutions of a monoclonal antibody (MAb) to the serotype III capsule to determine the presence of capsule in WT GBS (C) and the Δ ltdR mutant (D). A monoclonal antibody to the serotype Ia capsule was used as the isotype control. (F) Hemolysis assay comparing the hemolysis of sheep blood cells by WT GBS and the Δ ltdR mutant. Representative data from 1 of at least 2 independent experiments are shown. (G and H) Scanning electron microscopy images of WT GBS (G) and Δ ltdR mutant (H) strains. (I) Aggregation assay comparing aggregation of WT GBS, the Δ ltdR mutant, and the complemented strain in THB. (J) Clumping assay comparing clumping of the WT GBS, the Δ ltdR mutant, and the complemented strain in THB containing 0.1% fibrinogen. *, P
    Thb, supplied by Oxyrase Inc, used in various techniques. Bioz Stars score: 86/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 14 article reviews
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    thb - by Bioz Stars, 2020-04
    86/100 stars
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    91
    Carl Roth GmbH thb medium
    (A and B) Growth curves for WT <t>GBS</t> and the Δ ltdR mutant in <t>THB</t> (A) and CDM (B) at 37°C. (C to E) Flow cytometry using serial dilutions of a monoclonal antibody (MAb) to the serotype III capsule to determine the presence of capsule in WT GBS (C) and the Δ ltdR mutant (D). A monoclonal antibody to the serotype Ia capsule was used as the isotype control. (F) Hemolysis assay comparing the hemolysis of sheep blood cells by WT GBS and the Δ ltdR mutant. Representative data from 1 of at least 2 independent experiments are shown. (G and H) Scanning electron microscopy images of WT GBS (G) and Δ ltdR mutant (H) strains. (I) Aggregation assay comparing aggregation of WT GBS, the Δ ltdR mutant, and the complemented strain in THB. (J) Clumping assay comparing clumping of the WT GBS, the Δ ltdR mutant, and the complemented strain in THB containing 0.1% fibrinogen. *, P
    Thb Medium, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thb medium/product/Carl Roth GmbH
    Average 91 stars, based on 4 article reviews
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    91/100 stars
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    95
    IBI Scientific thb medium
    (A and B) Growth curves for WT <t>GBS</t> and the Δ ltdR mutant in <t>THB</t> (A) and CDM (B) at 37°C. (C to E) Flow cytometry using serial dilutions of a monoclonal antibody (MAb) to the serotype III capsule to determine the presence of capsule in WT GBS (C) and the Δ ltdR mutant (D). A monoclonal antibody to the serotype Ia capsule was used as the isotype control. (F) Hemolysis assay comparing the hemolysis of sheep blood cells by WT GBS and the Δ ltdR mutant. Representative data from 1 of at least 2 independent experiments are shown. (G and H) Scanning electron microscopy images of WT GBS (G) and Δ ltdR mutant (H) strains. (I) Aggregation assay comparing aggregation of WT GBS, the Δ ltdR mutant, and the complemented strain in THB. (J) Clumping assay comparing clumping of the WT GBS, the Δ ltdR mutant, and the complemented strain in THB containing 0.1% fibrinogen. *, P
    Thb Medium, supplied by IBI Scientific, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thb medium - by Bioz Stars, 2020-04
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    91
    Becton Dickinson thb
    (A and B) Growth curves for WT <t>GBS</t> and the Δ ltdR mutant in <t>THB</t> (A) and CDM (B) at 37°C. (C to E) Flow cytometry using serial dilutions of a monoclonal antibody (MAb) to the serotype III capsule to determine the presence of capsule in WT GBS (C) and the Δ ltdR mutant (D). A monoclonal antibody to the serotype Ia capsule was used as the isotype control. (F) Hemolysis assay comparing the hemolysis of sheep blood cells by WT GBS and the Δ ltdR mutant. Representative data from 1 of at least 2 independent experiments are shown. (G and H) Scanning electron microscopy images of WT GBS (G) and Δ ltdR mutant (H) strains. (I) Aggregation assay comparing aggregation of WT GBS, the Δ ltdR mutant, and the complemented strain in THB. (J) Clumping assay comparing clumping of the WT GBS, the Δ ltdR mutant, and the complemented strain in THB containing 0.1% fibrinogen. *, P
    Thb, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Carl Roth GmbH thb
    (A and B) Growth curves for WT <t>GBS</t> and the Δ ltdR mutant in <t>THB</t> (A) and CDM (B) at 37°C. (C to E) Flow cytometry using serial dilutions of a monoclonal antibody (MAb) to the serotype III capsule to determine the presence of capsule in WT GBS (C) and the Δ ltdR mutant (D). A monoclonal antibody to the serotype Ia capsule was used as the isotype control. (F) Hemolysis assay comparing the hemolysis of sheep blood cells by WT GBS and the Δ ltdR mutant. Representative data from 1 of at least 2 independent experiments are shown. (G and H) Scanning electron microscopy images of WT GBS (G) and Δ ltdR mutant (H) strains. (I) Aggregation assay comparing aggregation of WT GBS, the Δ ltdR mutant, and the complemented strain in THB. (J) Clumping assay comparing clumping of the WT GBS, the Δ ltdR mutant, and the complemented strain in THB containing 0.1% fibrinogen. *, P
    Thb, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thb - by Bioz Stars, 2020-04
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    93
    Qingdao Hope Bio thb
    (A and B) Growth curves for WT <t>GBS</t> and the Δ ltdR mutant in <t>THB</t> (A) and CDM (B) at 37°C. (C to E) Flow cytometry using serial dilutions of a monoclonal antibody (MAb) to the serotype III capsule to determine the presence of capsule in WT GBS (C) and the Δ ltdR mutant (D). A monoclonal antibody to the serotype Ia capsule was used as the isotype control. (F) Hemolysis assay comparing the hemolysis of sheep blood cells by WT GBS and the Δ ltdR mutant. Representative data from 1 of at least 2 independent experiments are shown. (G and H) Scanning electron microscopy images of WT GBS (G) and Δ ltdR mutant (H) strains. (I) Aggregation assay comparing aggregation of WT GBS, the Δ ltdR mutant, and the complemented strain in THB. (J) Clumping assay comparing clumping of the WT GBS, the Δ ltdR mutant, and the complemented strain in THB containing 0.1% fibrinogen. *, P
    Thb, supplied by Qingdao Hope Bio, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thb  (Celgene)
    87
    Celgene thb
    (A and B) Growth curves for WT <t>GBS</t> and the Δ ltdR mutant in <t>THB</t> (A) and CDM (B) at 37°C. (C to E) Flow cytometry using serial dilutions of a monoclonal antibody (MAb) to the serotype III capsule to determine the presence of capsule in WT GBS (C) and the Δ ltdR mutant (D). A monoclonal antibody to the serotype Ia capsule was used as the isotype control. (F) Hemolysis assay comparing the hemolysis of sheep blood cells by WT GBS and the Δ ltdR mutant. Representative data from 1 of at least 2 independent experiments are shown. (G and H) Scanning electron microscopy images of WT GBS (G) and Δ ltdR mutant (H) strains. (I) Aggregation assay comparing aggregation of WT GBS, the Δ ltdR mutant, and the complemented strain in THB. (J) Clumping assay comparing clumping of the WT GBS, the Δ ltdR mutant, and the complemented strain in THB containing 0.1% fibrinogen. *, P
    Thb, supplied by Celgene, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thb  (Olympus)
    87
    Olympus thb
    (A and B) Growth curves for WT <t>GBS</t> and the Δ ltdR mutant in <t>THB</t> (A) and CDM (B) at 37°C. (C to E) Flow cytometry using serial dilutions of a monoclonal antibody (MAb) to the serotype III capsule to determine the presence of capsule in WT GBS (C) and the Δ ltdR mutant (D). A monoclonal antibody to the serotype Ia capsule was used as the isotype control. (F) Hemolysis assay comparing the hemolysis of sheep blood cells by WT GBS and the Δ ltdR mutant. Representative data from 1 of at least 2 independent experiments are shown. (G and H) Scanning electron microscopy images of WT GBS (G) and Δ ltdR mutant (H) strains. (I) Aggregation assay comparing aggregation of WT GBS, the Δ ltdR mutant, and the complemented strain in THB. (J) Clumping assay comparing clumping of the WT GBS, the Δ ltdR mutant, and the complemented strain in THB containing 0.1% fibrinogen. *, P
    Thb, supplied by Olympus, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Corning Life Sciences thb
    (A and B) Growth curves for WT <t>GBS</t> and the Δ ltdR mutant in <t>THB</t> (A) and CDM (B) at 37°C. (C to E) Flow cytometry using serial dilutions of a monoclonal antibody (MAb) to the serotype III capsule to determine the presence of capsule in WT GBS (C) and the Δ ltdR mutant (D). A monoclonal antibody to the serotype Ia capsule was used as the isotype control. (F) Hemolysis assay comparing the hemolysis of sheep blood cells by WT GBS and the Δ ltdR mutant. Representative data from 1 of at least 2 independent experiments are shown. (G and H) Scanning electron microscopy images of WT GBS (G) and Δ ltdR mutant (H) strains. (I) Aggregation assay comparing aggregation of WT GBS, the Δ ltdR mutant, and the complemented strain in THB. (J) Clumping assay comparing clumping of the WT GBS, the Δ ltdR mutant, and the complemented strain in THB containing 0.1% fibrinogen. *, P
    Thb, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A and B) Growth curves for WT <t>GBS</t> and the Δ ltdR mutant in <t>THB</t> (A) and CDM (B) at 37°C. (C to E) Flow cytometry using serial dilutions of a monoclonal antibody (MAb) to the serotype III capsule to determine the presence of capsule in WT GBS (C) and the Δ ltdR mutant (D). A monoclonal antibody to the serotype Ia capsule was used as the isotype control. (F) Hemolysis assay comparing the hemolysis of sheep blood cells by WT GBS and the Δ ltdR mutant. Representative data from 1 of at least 2 independent experiments are shown. (G and H) Scanning electron microscopy images of WT GBS (G) and Δ ltdR mutant (H) strains. (I) Aggregation assay comparing aggregation of WT GBS, the Δ ltdR mutant, and the complemented strain in THB. (J) Clumping assay comparing clumping of the WT GBS, the Δ ltdR mutant, and the complemented strain in THB containing 0.1% fibrinogen. *, P
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    Color phenotypes of strains expressing phoZ derivatives and grown as colonies on XP agar. (Top) E. coli CC873 expressing the periplasmic chimera LacY P1 ::AP, the cytoplasmic chimera LacY C2 ::AP, or the unfused LacY C2 or LacY P1 proteins (no AP). Strains were grown on Luria agar containing the chromogenic substrate XP. (Bottom). Gram-positive species deficient in AP (NONE) or expressing wild-type AP (WT), AP deleted of its signal sequence (NO S.S), or AP with the C5A peptidase signal sequence ( scpB S.S). Strains were grown on <t>Todd-Hewitt</t> agar with XP. On this medium, the GAS strains (insert) grew relatively slowly and had to be photographed 24 h after the other species.
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    Image Search Results


    Growth of S. dysgalactiae subsp. equisimilis in the presence of LL-37 and DrsG. S. dysgalactiae subsp. equisimilis MD985 ( drsG negative) was grown in the presence of LL-37 and/or DrsGL (A), DrsGS (B), or SIC (C). After a 2.5-h incubation, the bacteria were recovered, plated onto Todd-Hewitt agar, and incubated overnight. The percentage of growth compared to that of controls was determined for each individual assay. The results presented are the means for three independent experiments.

    Journal: Infection and Immunity

    Article Title: DrsG from Streptococcus dysgalactiae subsp. equisimilis Inhibits the Antimicrobial Peptide LL-37

    doi: 10.1128/IAI.01411-13

    Figure Lengend Snippet: Growth of S. dysgalactiae subsp. equisimilis in the presence of LL-37 and DrsG. S. dysgalactiae subsp. equisimilis MD985 ( drsG negative) was grown in the presence of LL-37 and/or DrsGL (A), DrsGS (B), or SIC (C). After a 2.5-h incubation, the bacteria were recovered, plated onto Todd-Hewitt agar, and incubated overnight. The percentage of growth compared to that of controls was determined for each individual assay. The results presented are the means for three independent experiments.

    Article Snippet: Unless otherwise specified, S. dysgalactiae subsp. equisimilis and S. pyogenes were grown in Todd-Hewitt broth (THB), Todd-Hewitt agar (Oxoid), or Columbia agar (CBA; Oxoid) supplemented with 5% defibrinated horse blood.

    Techniques: Incubation

    Transfection of CD21 into Ramos cells impedes anti-CD19 uptake. Upper panels: freshly isolated human B-cells do not internalize Alexa555-labeled anti-CD19 within 3 h (A, red channel in C), although it does redistribute into patches on the cell surface (arrows) that co-localize with Alexa488-labeled anti-CD21 added post-uptake on ice (B, green channel in C). Scale bar = 20 μm. Lower panels: Ramos cells (left panels) stably expressing a high (clone 1, right panels) or medium (clone 3, middle panels) level of CD21 were incubated with Alexa555-labeled anti-CD19 (G–I and red channel in M–O) and Alexa647-transferrin (J–L and M–O, shown in green channel for better contrast) for 3 h at 37°C, then chilled and incubated with Alexa488-conjugated anti-CD21 antibodies (D–F) on ice prior to fixation and imaging. Anti-CD19 uptake is impeded by increased CD21 expression, while transferrin uptake is unaffected. Internalized anti-CD19 antibodies do not significantly co-localize with the recycling transferrin at this time-point, as seen by lack of yellow colour in the respective overlaid images (M–O). Gamma levels were adjusted where required for clarity. Scale bar = 20 μm.

    Journal: British Journal of Haematology

    Article Title: High CD21 expression inhibits internalization of anti-CD19 antibodies and cytotoxicity of an anti-CD19-drug conjugate

    doi: 10.1111/j.1365-2141.2007.06883.x

    Figure Lengend Snippet: Transfection of CD21 into Ramos cells impedes anti-CD19 uptake. Upper panels: freshly isolated human B-cells do not internalize Alexa555-labeled anti-CD19 within 3 h (A, red channel in C), although it does redistribute into patches on the cell surface (arrows) that co-localize with Alexa488-labeled anti-CD21 added post-uptake on ice (B, green channel in C). Scale bar = 20 μm. Lower panels: Ramos cells (left panels) stably expressing a high (clone 1, right panels) or medium (clone 3, middle panels) level of CD21 were incubated with Alexa555-labeled anti-CD19 (G–I and red channel in M–O) and Alexa647-transferrin (J–L and M–O, shown in green channel for better contrast) for 3 h at 37°C, then chilled and incubated with Alexa488-conjugated anti-CD21 antibodies (D–F) on ice prior to fixation and imaging. Anti-CD19 uptake is impeded by increased CD21 expression, while transferrin uptake is unaffected. Internalized anti-CD19 antibodies do not significantly co-localize with the recycling transferrin at this time-point, as seen by lack of yellow colour in the respective overlaid images (M–O). Gamma levels were adjusted where required for clarity. Scale bar = 20 μm.

    Article Snippet: Antibodies Unless otherwise indicated, antibodies used were mouse anti-CD19 (clone B496, Biomeda CB-19; Biomeda, Foster City, CA, USA) and mouse anti-CD21 [HB135 (American Type Culture Collection, Manassas, VA, USA), also called THB-5 or HB5], anti-CD20 (2H7), anti-CD22 (RFB4) and anti-CD79b (SN8), all affinity purified at Genentech Inc. (South San Francisco, CA, USA) from hybridoma supernatants.

    Techniques: Transfection, Isolation, Labeling, Stable Transfection, Expressing, Incubation, Imaging

    Anti-CD21 antibodies are not significantly internalized, while anti-CD19 antibodies only internalize readily in CD21 lo or CD21 − cells. Various B-cell lines were incubated with anti-CD21 (HB135) for 20 h at 37°C in the presence of lysosomal protease inhibitors, and the total antibody distribution detected post fixation and permeabilization with Cy3-conjugated anti-mouse (left panels). Insets show surface binding of anti-CD21 following 1 h incubation on ice. Ramos (A) and DoHH2 (B) cells lack surface expression of CD21 and consequently failed to internalize any antibody, as expected. Anti-CD21 is not significantly internalized in the low CD21-expressing Namalwa (C) or Daudi (D) cells, or even in the higher expressing ARH77 (E) or Raji (F) cells, or in freshly isolated primary human B-cells (G). The same cell lines were incubated with anti-CD19 (B496) antibodies on ice for 1 h (insets in middle panels), or at 37°C for 3 h (middle panels) or 20 h (right panels) with detection as above. The CD21-negative cell lines Ramos (H,O) and DoHH2 (I,P) readily internalized anti-CD19 within 3 h, while the low CD21-expressing Namalwa (J,Q) and Daudi (K,R) cells internalized it less extensively, as judged by the faint plasma membrane staining remaining even after 20 h uptake. The high CD21-expressors, ARH77 and Raji did not detectably internalize anti-CD19 after 3 h (L,M), and after 20 h still had not internalized nearly as much as the CD21-negative cells did in 3 h (S,T). Primary human B-cells did not internalize anti-CD19 within 3 h (N), but did by 20 h (U). Virtually all the cells in each field readily internalized Alexa488-transferrin (with the exception of transferrin-receptor negative primary B-cells), indicating that any lack of antibody uptake was not due to loss of viability (not shown). Gamma levels were adjusted where appropriate. Scale bar = 20 μm.

    Journal: British Journal of Haematology

    Article Title: High CD21 expression inhibits internalization of anti-CD19 antibodies and cytotoxicity of an anti-CD19-drug conjugate

    doi: 10.1111/j.1365-2141.2007.06883.x

    Figure Lengend Snippet: Anti-CD21 antibodies are not significantly internalized, while anti-CD19 antibodies only internalize readily in CD21 lo or CD21 − cells. Various B-cell lines were incubated with anti-CD21 (HB135) for 20 h at 37°C in the presence of lysosomal protease inhibitors, and the total antibody distribution detected post fixation and permeabilization with Cy3-conjugated anti-mouse (left panels). Insets show surface binding of anti-CD21 following 1 h incubation on ice. Ramos (A) and DoHH2 (B) cells lack surface expression of CD21 and consequently failed to internalize any antibody, as expected. Anti-CD21 is not significantly internalized in the low CD21-expressing Namalwa (C) or Daudi (D) cells, or even in the higher expressing ARH77 (E) or Raji (F) cells, or in freshly isolated primary human B-cells (G). The same cell lines were incubated with anti-CD19 (B496) antibodies on ice for 1 h (insets in middle panels), or at 37°C for 3 h (middle panels) or 20 h (right panels) with detection as above. The CD21-negative cell lines Ramos (H,O) and DoHH2 (I,P) readily internalized anti-CD19 within 3 h, while the low CD21-expressing Namalwa (J,Q) and Daudi (K,R) cells internalized it less extensively, as judged by the faint plasma membrane staining remaining even after 20 h uptake. The high CD21-expressors, ARH77 and Raji did not detectably internalize anti-CD19 after 3 h (L,M), and after 20 h still had not internalized nearly as much as the CD21-negative cells did in 3 h (S,T). Primary human B-cells did not internalize anti-CD19 within 3 h (N), but did by 20 h (U). Virtually all the cells in each field readily internalized Alexa488-transferrin (with the exception of transferrin-receptor negative primary B-cells), indicating that any lack of antibody uptake was not due to loss of viability (not shown). Gamma levels were adjusted where appropriate. Scale bar = 20 μm.

    Article Snippet: Antibodies Unless otherwise indicated, antibodies used were mouse anti-CD19 (clone B496, Biomeda CB-19; Biomeda, Foster City, CA, USA) and mouse anti-CD21 [HB135 (American Type Culture Collection, Manassas, VA, USA), also called THB-5 or HB5], anti-CD20 (2H7), anti-CD22 (RFB4) and anti-CD79b (SN8), all affinity purified at Genentech Inc. (South San Francisco, CA, USA) from hybridoma supernatants.

    Techniques: Incubation, Binding Assay, Expressing, Isolation, Staining

    Quantitation of CD19 and CD21 surface levels and anti-CD19 uptake by flow cytometry confirms the immunofluorescence results. (A) B-cell lines were incubated on ice with 2 μg/ml mouse anti-CD21 (HB135) or mouse anti-CD19 (B496), followed by rat anti-mouse-phycoerythrin and analyzed by flow cytometry to determine surface expression. Results are the average mean fluorescence intensity (MFI) of triplicates ± standard deviation from a representative of three independent experiments (average of five independent experiments shown for the more variable ARH77 cells). Shown in increasing order of CD21 expression are: (1) SuDHL-4, (2) Ramos, (3) DoHH2, (4) Namalwa, (5) Daudi, (6) Ramos-CD21 clone 3, (7) ARH77, (8) Raji, (9) Ramos-CD21 clone 1. (10) Freshly isolated human B-cells have lower fluorescence values for both antigens than expected due to their small size, but their relative ratio of CD21 to CD19 is similar to that of ARH77 and Raji cells. Ramos-CD21 clone 1 expresses CD21 even more highly than Raji, while Ramos-CD21 clone 3 is intermediate between that of ARH77 and Daudi. (B) The rate of internalization of Alexa488-anti-CD19 in Ramos (▪), Ramos-CD21 clone 1 (□), Ramos-CD21 clone 3 (△) and CD21 hi ARH77 (▴) cells was determined by pre-binding to cells then incubating at 37°C (without washing) for the indicated times, washing and fixing either with or without surface fluorescence quenching with anti-Alexa488. Results are the average and standard deviation of two duplicate experiments each normalized to their respective initial surface binding levels after subtraction of background signals.

    Journal: British Journal of Haematology

    Article Title: High CD21 expression inhibits internalization of anti-CD19 antibodies and cytotoxicity of an anti-CD19-drug conjugate

    doi: 10.1111/j.1365-2141.2007.06883.x

    Figure Lengend Snippet: Quantitation of CD19 and CD21 surface levels and anti-CD19 uptake by flow cytometry confirms the immunofluorescence results. (A) B-cell lines were incubated on ice with 2 μg/ml mouse anti-CD21 (HB135) or mouse anti-CD19 (B496), followed by rat anti-mouse-phycoerythrin and analyzed by flow cytometry to determine surface expression. Results are the average mean fluorescence intensity (MFI) of triplicates ± standard deviation from a representative of three independent experiments (average of five independent experiments shown for the more variable ARH77 cells). Shown in increasing order of CD21 expression are: (1) SuDHL-4, (2) Ramos, (3) DoHH2, (4) Namalwa, (5) Daudi, (6) Ramos-CD21 clone 3, (7) ARH77, (8) Raji, (9) Ramos-CD21 clone 1. (10) Freshly isolated human B-cells have lower fluorescence values for both antigens than expected due to their small size, but their relative ratio of CD21 to CD19 is similar to that of ARH77 and Raji cells. Ramos-CD21 clone 1 expresses CD21 even more highly than Raji, while Ramos-CD21 clone 3 is intermediate between that of ARH77 and Daudi. (B) The rate of internalization of Alexa488-anti-CD19 in Ramos (▪), Ramos-CD21 clone 1 (□), Ramos-CD21 clone 3 (△) and CD21 hi ARH77 (▴) cells was determined by pre-binding to cells then incubating at 37°C (without washing) for the indicated times, washing and fixing either with or without surface fluorescence quenching with anti-Alexa488. Results are the average and standard deviation of two duplicate experiments each normalized to their respective initial surface binding levels after subtraction of background signals.

    Article Snippet: Antibodies Unless otherwise indicated, antibodies used were mouse anti-CD19 (clone B496, Biomeda CB-19; Biomeda, Foster City, CA, USA) and mouse anti-CD21 [HB135 (American Type Culture Collection, Manassas, VA, USA), also called THB-5 or HB5], anti-CD20 (2H7), anti-CD22 (RFB4) and anti-CD79b (SN8), all affinity purified at Genentech Inc. (South San Francisco, CA, USA) from hybridoma supernatants.

    Techniques: Quantitation Assay, Flow Cytometry, Cytometry, Immunofluorescence, Incubation, Expressing, Fluorescence, Standard Deviation, Isolation, Binding Assay

    Anti-CD19-MCC-DM1 is less efficacious in high CD21 expressing cells. (A) CD21 hi Raji were incubated for 3 d with anti-CD21-MCC-DM1 (○), negative control Trastuzumab-MCC-DM1 (▴), free L-DM1 dimer (▪) or naked anti-CD21 antibodies (•) and assessed for viability by measuring ATP levels. CD21 hi ARH77 (B), CD21 lo Daudi (C), and CD21 − DoHH2 cells (D) were incubated for 3 d with anti-CD19-MCC-DM1 (♦), negative control Trastuzumab-MCC-DM1 (▴), free L-DM1 dimer (▪) or naked anti-CD19 antibodies ( × ) and assessed for viability by measuring ATP levels. (E) Ramos (solid symbols and lines) and Ramos-CD21 clone 1 (open symbols and dashed lines) were treated with anti-CD19-MCC-DM1 (♦,⋄) or free DM1 (▪,□) as in B-D. Inset bar graph shows percentage killing of Ramos (♦) and Ramos-CD21 clone 1 (⋄) at the highest anti-CD19-MCC-DM1 concentration used (3·33 μg/ml). (F) Ramos (solid symbols and lines) and Ramos-CD21 clone 1 (open symbols and dashed lines) were treated with control Trastuzumab-MCC-DM1 (▴,△), free DM1 (▪,□) or unconjugated anti-CD19 ( × ) as in B. Data are shown in all panels as a percentage viability of untreated control cells (mean and standard deviation of three independent duplicate experiments) versus ADC concentration in μg/ml on the lower x -axes or free DM1 concentration in M on the upper x -axes. * denotes data points statistically different ( P

    Journal: British Journal of Haematology

    Article Title: High CD21 expression inhibits internalization of anti-CD19 antibodies and cytotoxicity of an anti-CD19-drug conjugate

    doi: 10.1111/j.1365-2141.2007.06883.x

    Figure Lengend Snippet: Anti-CD19-MCC-DM1 is less efficacious in high CD21 expressing cells. (A) CD21 hi Raji were incubated for 3 d with anti-CD21-MCC-DM1 (○), negative control Trastuzumab-MCC-DM1 (▴), free L-DM1 dimer (▪) or naked anti-CD21 antibodies (•) and assessed for viability by measuring ATP levels. CD21 hi ARH77 (B), CD21 lo Daudi (C), and CD21 − DoHH2 cells (D) were incubated for 3 d with anti-CD19-MCC-DM1 (♦), negative control Trastuzumab-MCC-DM1 (▴), free L-DM1 dimer (▪) or naked anti-CD19 antibodies ( × ) and assessed for viability by measuring ATP levels. (E) Ramos (solid symbols and lines) and Ramos-CD21 clone 1 (open symbols and dashed lines) were treated with anti-CD19-MCC-DM1 (♦,⋄) or free DM1 (▪,□) as in B-D. Inset bar graph shows percentage killing of Ramos (♦) and Ramos-CD21 clone 1 (⋄) at the highest anti-CD19-MCC-DM1 concentration used (3·33 μg/ml). (F) Ramos (solid symbols and lines) and Ramos-CD21 clone 1 (open symbols and dashed lines) were treated with control Trastuzumab-MCC-DM1 (▴,△), free DM1 (▪,□) or unconjugated anti-CD19 ( × ) as in B. Data are shown in all panels as a percentage viability of untreated control cells (mean and standard deviation of three independent duplicate experiments) versus ADC concentration in μg/ml on the lower x -axes or free DM1 concentration in M on the upper x -axes. * denotes data points statistically different ( P

    Article Snippet: Antibodies Unless otherwise indicated, antibodies used were mouse anti-CD19 (clone B496, Biomeda CB-19; Biomeda, Foster City, CA, USA) and mouse anti-CD21 [HB135 (American Type Culture Collection, Manassas, VA, USA), also called THB-5 or HB5], anti-CD20 (2H7), anti-CD22 (RFB4) and anti-CD79b (SN8), all affinity purified at Genentech Inc. (South San Francisco, CA, USA) from hybridoma supernatants.

    Techniques: Expressing, Incubation, Negative Control, Concentration Assay, Standard Deviation

    Scanning electron microscopy of biofilm of S. suis grown in THB broth. (A) With 0 MIC (0 μg mL -1 ) concentration of aqueous extracts of Rhizoma Coptidis ; (B) with 1/2 × MIC (50 μg mL -1 ) concentration of aqueous extracts of Rhizoma Coptidis .

    Journal: Frontiers in Pharmacology

    Article Title: Inhibition of Streptococcus suis Adhesion and Biofilm Formation in Vitro by Water Extracts of Rhizoma Coptidis

    doi: 10.3389/fphar.2018.00371

    Figure Lengend Snippet: Scanning electron microscopy of biofilm of S. suis grown in THB broth. (A) With 0 MIC (0 μg mL -1 ) concentration of aqueous extracts of Rhizoma Coptidis ; (B) with 1/2 × MIC (50 μg mL -1 ) concentration of aqueous extracts of Rhizoma Coptidis .

    Article Snippet: Growth Conditions of S. suis Biofilms Streptococcus suis ATCC700794 was grown overnight in THB (Sigma-Aldrich) at 37°C with constant shaking.

    Techniques: Electron Microscopy, Concentration Assay

    Stability of plasmid pGU2664 in the absence of selection. (A and B) Numbers of Sp r GBS (red) and the total numbers of GBS (black) recovered from growth conditions with (A) and without (B) antibiotic selection, respectively. Cells were diluted 1:1,000 into fresh THB medium (±Sp) and grown for 12 h in 5 successive culture passages, representing approximately 100 generations. The results presented are averages of the results from three independent experiments compared using AUC Student's t test, with bars showing the standard error.

    Journal: Applied and Environmental Microbiology

    Article Title: Stable Expression of Modified Green Fluorescent Protein in Group B Streptococci To Enable Visualization in Experimental Systems

    doi: 10.1128/AEM.01262-18

    Figure Lengend Snippet: Stability of plasmid pGU2664 in the absence of selection. (A and B) Numbers of Sp r GBS (red) and the total numbers of GBS (black) recovered from growth conditions with (A) and without (B) antibiotic selection, respectively. Cells were diluted 1:1,000 into fresh THB medium (±Sp) and grown for 12 h in 5 successive culture passages, representing approximately 100 generations. The results presented are averages of the results from three independent experiments compared using AUC Student's t test, with bars showing the standard error.

    Article Snippet: E. coli strains were routinely cultured in lysogeny broth (LB), and GBS strains were routinely cultured in THB (Thermo Fisher Scientific), on tryptone soya agar (TSA; Thermo Fisher Scientific) containing 5% horse blood (Thermo Fisher Scientific) or in CDM ( ).

    Techniques: Plasmid Preparation, Selection

    Measuring GBS growth in THB using plasmid pGU2664. (A) Culture turbidity of GBS GU2666 (GFPmut3+ plasmid pGU2664; green) and GU2672 (empty vector pDL278; black) grown in THB medium. (B) The fluorescence intensity of GFPmut3 was monitored simultaneously. (C) Fluorescence polarization (FP) intensity measurements were used to distinguish GFPmut3 intensity from background autofluorescence of THB medium. Mean and SEM data are representative of at least three independent replicates. RFU, relative fluorescence units.

    Journal: Applied and Environmental Microbiology

    Article Title: Stable Expression of Modified Green Fluorescent Protein in Group B Streptococci To Enable Visualization in Experimental Systems

    doi: 10.1128/AEM.01262-18

    Figure Lengend Snippet: Measuring GBS growth in THB using plasmid pGU2664. (A) Culture turbidity of GBS GU2666 (GFPmut3+ plasmid pGU2664; green) and GU2672 (empty vector pDL278; black) grown in THB medium. (B) The fluorescence intensity of GFPmut3 was monitored simultaneously. (C) Fluorescence polarization (FP) intensity measurements were used to distinguish GFPmut3 intensity from background autofluorescence of THB medium. Mean and SEM data are representative of at least three independent replicates. RFU, relative fluorescence units.

    Article Snippet: E. coli strains were routinely cultured in lysogeny broth (LB), and GBS strains were routinely cultured in THB (Thermo Fisher Scientific), on tryptone soya agar (TSA; Thermo Fisher Scientific) containing 5% horse blood (Thermo Fisher Scientific) or in CDM ( ).

    Techniques: Plasmid Preparation, Fluorescence

    (A and B) Growth curves for WT GBS and the Δ ltdR mutant in THB (A) and CDM (B) at 37°C. (C to E) Flow cytometry using serial dilutions of a monoclonal antibody (MAb) to the serotype III capsule to determine the presence of capsule in WT GBS (C) and the Δ ltdR mutant (D). A monoclonal antibody to the serotype Ia capsule was used as the isotype control. (F) Hemolysis assay comparing the hemolysis of sheep blood cells by WT GBS and the Δ ltdR mutant. Representative data from 1 of at least 2 independent experiments are shown. (G and H) Scanning electron microscopy images of WT GBS (G) and Δ ltdR mutant (H) strains. (I) Aggregation assay comparing aggregation of WT GBS, the Δ ltdR mutant, and the complemented strain in THB. (J) Clumping assay comparing clumping of the WT GBS, the Δ ltdR mutant, and the complemented strain in THB containing 0.1% fibrinogen. *, P

    Journal: Infection and Immunity

    Article Title: Characterization of a Two-Component System Transcriptional Regulator, LtdR, That Impacts Group B Streptococcal Colonization and Disease

    doi: 10.1128/IAI.00822-17

    Figure Lengend Snippet: (A and B) Growth curves for WT GBS and the Δ ltdR mutant in THB (A) and CDM (B) at 37°C. (C to E) Flow cytometry using serial dilutions of a monoclonal antibody (MAb) to the serotype III capsule to determine the presence of capsule in WT GBS (C) and the Δ ltdR mutant (D). A monoclonal antibody to the serotype Ia capsule was used as the isotype control. (F) Hemolysis assay comparing the hemolysis of sheep blood cells by WT GBS and the Δ ltdR mutant. Representative data from 1 of at least 2 independent experiments are shown. (G and H) Scanning electron microscopy images of WT GBS (G) and Δ ltdR mutant (H) strains. (I) Aggregation assay comparing aggregation of WT GBS, the Δ ltdR mutant, and the complemented strain in THB. (J) Clumping assay comparing clumping of the WT GBS, the Δ ltdR mutant, and the complemented strain in THB containing 0.1% fibrinogen. *, P

    Article Snippet: The GBS strains were grown in THB (Hardy Diagnostics) at 37°C, and growth was monitored by measuring the optical density at 600 nm (OD600 ).

    Techniques: Mutagenesis, Flow Cytometry, Cytometry, IA, Hemolysis Assay, Electron Microscopy

    Scanning electron micrographs of S. suis ATCC700794 biofilm following growth in THB supplemented without azithromycin [ (A or C) , control], or with 1/2 MIC of azithromycin (B or D) . Controls refer to the absence of azithromycin.

    Journal: Frontiers in Microbiology

    Article Title: Sub-MICs of Azithromycin Decrease Biofilm Formation of Streptococcus suis and Increase Capsular Polysaccharide Content of S. suis

    doi: 10.3389/fmicb.2016.01659

    Figure Lengend Snippet: Scanning electron micrographs of S. suis ATCC700794 biofilm following growth in THB supplemented without azithromycin [ (A or C) , control], or with 1/2 MIC of azithromycin (B or D) . Controls refer to the absence of azithromycin.

    Article Snippet: Briefly, S. suis ATCC700794 was grown aerobically at 37°C in THB (Summus Ltd, Harbin, Heilongjiang, China) overnight.

    Techniques:

    Color phenotypes of strains expressing phoZ derivatives and grown as colonies on XP agar. (Top) E. coli CC873 expressing the periplasmic chimera LacY P1 ::AP, the cytoplasmic chimera LacY C2 ::AP, or the unfused LacY C2 or LacY P1 proteins (no AP). Strains were grown on Luria agar containing the chromogenic substrate XP. (Bottom). Gram-positive species deficient in AP (NONE) or expressing wild-type AP (WT), AP deleted of its signal sequence (NO S.S), or AP with the C5A peptidase signal sequence ( scpB S.S). Strains were grown on Todd-Hewitt agar with XP. On this medium, the GAS strains (insert) grew relatively slowly and had to be photographed 24 h after the other species.

    Journal: Journal of Bacteriology

    Article Title: Characterization of Enterococcus faecalis Alkaline Phosphatase and Use in Identifying Streptococcus agalactiae Secreted Proteins

    doi:

    Figure Lengend Snippet: Color phenotypes of strains expressing phoZ derivatives and grown as colonies on XP agar. (Top) E. coli CC873 expressing the periplasmic chimera LacY P1 ::AP, the cytoplasmic chimera LacY C2 ::AP, or the unfused LacY C2 or LacY P1 proteins (no AP). Strains were grown on Luria agar containing the chromogenic substrate XP. (Bottom). Gram-positive species deficient in AP (NONE) or expressing wild-type AP (WT), AP deleted of its signal sequence (NO S.S), or AP with the C5A peptidase signal sequence ( scpB S.S). Strains were grown on Todd-Hewitt agar with XP. On this medium, the GAS strains (insert) grew relatively slowly and had to be photographed 24 h after the other species.

    Article Snippet: COH31 r/s was grown in Todd-Hewitt broth (THB) (Difco) supplemented with 10 μg of chloramphenicol/ml as required.

    Techniques: Expressing, Sequencing