Journal: Molecular cancer therapeutics
Article Title: NEO212 Inhibits Migration and Invasion of Glioma Stem Cells
Figure Lengend Snippet: The cytotoxic and anti-migratory effects of NEO212 are independent (A–C) USC02 cells recover their ability to migrate after removal of NEO212. (A) Representative images taken 72 h after performing the wound healing assay. Primary cultures of USC02 cells were treated with the indicated concentrations of NEO212 for 24 h, and medium was replaced with fresh medium without compound in half of the cells (upper panel), while in the other half the medium was not changed (lower panel). Scale bar, 200 μm. (B) After 72 h, the remaining wound area was quantified and compared to the corresponding initial area. (C) Cell viability after 72 h, relative to vehicle-treated cells whose medium was not changed after 24 h. (D-F) NEO212 losses its cytotoxic effects, but not its ability to block cell migration after 24 h of incubation in medium without cells. (D) Cell viability of USC02 after 120 h treatments with increasing concentrations of fresh NEO212 (black line) or NEO212 previously incubated for 24 h in medium at 37 °C and then added to the cells (grey line). (E) Wound healing assay performed with the indicated concentrations of fresh NEO212 (NEO212-0h, black bars) or NEO212 previously incubated in medium for 24 h at 37 °C (NEO212-24h, grey bars). After 24 h, the remaining wound area was quantified, and represented as relative to each corresponding initial area. (F) Representative images taken at 0 h (upper panel) and 24 h (lower panel), of the wound healing assay performed with USC02 cells at the indicated concentrations of NEO212 previously pre-incubated during 24 h at 37 °C. Scale bar, 200 μm. In all cases, the bar graphs represent the average ± SEM of at least three independent experiments performed in quadruplicate. *, P
Article Snippet: Here, we show that NEO212 effectively reverses the EMT process, mainly by upregulating several genes that are normally downregulated during EMT , such as cell-cell adhesion molecules CDH1, DSC2, DSP; epithelial cytokeratins KRT7 and KRT19[ , ]; secreted factors that promote an epithelial-like phenotype[ ] like FGFBP1, IL1RN, SPP1, TFPI-2, ESR1 and MST1R; as well as BMP2 and Wnt11, both related to cell differentiation and development[ , ].
Techniques: Wound Healing Assay, Blocking Assay, Migration, Incubation