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  • 99
    Gyros Protein Technologies tfa
    Tfa, supplied by Gyros Protein Technologies, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tfa/product/Gyros Protein Technologies
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    tfa - by Bioz Stars, 2020-03
    99/100 stars
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    99
    Thermo Fisher trifluroacetic acid tfa
    Trifluroacetic Acid Tfa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trifluroacetic acid tfa/product/Thermo Fisher
    Average 99 stars, based on 14 article reviews
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    trifluroacetic acid tfa - by Bioz Stars, 2020-03
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    99
    Thermo Fisher pierce trifluoracetic acid
    Pierce Trifluoracetic Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pierce trifluoracetic acid/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
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    79
    Biosolve trifluroacetic acid tfa
    Capillary HILIC-MS of an E. coli lysate (5 μL loaded of a 2.5 mg/mL solution in 2% <t>ACN</t> and 0.1% <t>TFA).</t> (top) Base-peak chromatogram (900–3000 m / z ); (bottom) feature map showing deconvoluted MS spectra. The deconvolution algorithm is based on isotopically resolved molecular features, and therefore, potential features above 35 kDa are not identified. Mobile phases as specified in the Experimental Section . Loading at 10% B, multisegment linear gradient from 10–12% B in 1 min, 12–30% in 30 min, 30–65% B in 6 min, 65–90% B in 1 min, followed by 3 min at 90% B and several washing steps (total analysis time of 60 min).
    Trifluroacetic Acid Tfa, supplied by Biosolve, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    J&K Scientific trifluoracetic acid tfa
    Capillary HILIC-MS of an E. coli lysate (5 μL loaded of a 2.5 mg/mL solution in 2% <t>ACN</t> and 0.1% <t>TFA).</t> (top) Base-peak chromatogram (900–3000 m / z ); (bottom) feature map showing deconvoluted MS spectra. The deconvolution algorithm is based on isotopically resolved molecular features, and therefore, potential features above 35 kDa are not identified. Mobile phases as specified in the Experimental Section . Loading at 10% B, multisegment linear gradient from 10–12% B in 1 min, 12–30% in 30 min, 30–65% B in 6 min, 65–90% B in 1 min, followed by 3 min at 90% B and several washing steps (total analysis time of 60 min).
    Trifluoracetic Acid Tfa, supplied by J&K Scientific, used in various techniques. Bioz Stars score: 81/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    trifluoracetic acid tfa - by Bioz Stars, 2020-03
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    81
    abcr GmbH trifluoracetic acid tfa
    Capillary HILIC-MS of an E. coli lysate (5 μL loaded of a 2.5 mg/mL solution in 2% <t>ACN</t> and 0.1% <t>TFA).</t> (top) Base-peak chromatogram (900–3000 m / z ); (bottom) feature map showing deconvoluted MS spectra. The deconvolution algorithm is based on isotopically resolved molecular features, and therefore, potential features above 35 kDa are not identified. Mobile phases as specified in the Experimental Section . Loading at 10% B, multisegment linear gradient from 10–12% B in 1 min, 12–30% in 30 min, 30–65% B in 6 min, 65–90% B in 1 min, followed by 3 min at 90% B and several washing steps (total analysis time of 60 min).
    Trifluoracetic Acid Tfa, supplied by abcr GmbH, used in various techniques. Bioz Stars score: 81/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 81 stars, based on 5 article reviews
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    78
    Millipore tfa trifluroacetic acid
    Enzymatic degradation . Analytical <t>HPLC</t> chromatograms of HVP (a), 2HVP (b), DHVP (c) and D-2HVP (d), in 10% FBS at 37 °C after 5 h. Dotted lines indicate the retention time of each peptide in water at the same conditions of analysis. Other peaks in the chromatograms are due to serum components. Conditions: Column, Jupiter C 18 “proteo” (4 μm, 90 Å, 4.6 × 250 mm); flow, 1 mL/min; eluent A, 0.05% <t>TFA</t> in H 2 O milliQ; eluent B, 0.05% TFA in CH 3 CN; injection, 200 μL; gradient, from 0 to 30% of B in 60 min; λ = 214 nm.
    Tfa Trifluroacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore trifluroacetic acid tfa sigma
    Enzymatic degradation . Analytical <t>HPLC</t> chromatograms of HVP (a), 2HVP (b), DHVP (c) and D-2HVP (d), in 10% FBS at 37 °C after 5 h. Dotted lines indicate the retention time of each peptide in water at the same conditions of analysis. Other peaks in the chromatograms are due to serum components. Conditions: Column, Jupiter C 18 “proteo” (4 μm, 90 Å, 4.6 × 250 mm); flow, 1 mL/min; eluent A, 0.05% <t>TFA</t> in H 2 O milliQ; eluent B, 0.05% TFA in CH 3 CN; injection, 200 μL; gradient, from 0 to 30% of B in 60 min; λ = 214 nm.
    Trifluroacetic Acid Tfa Sigma, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore deuterated trifluoracetic acid tfa d
    Enzymatic degradation . Analytical <t>HPLC</t> chromatograms of HVP (a), 2HVP (b), DHVP (c) and D-2HVP (d), in 10% FBS at 37 °C after 5 h. Dotted lines indicate the retention time of each peptide in water at the same conditions of analysis. Other peaks in the chromatograms are due to serum components. Conditions: Column, Jupiter C 18 “proteo” (4 μm, 90 Å, 4.6 × 250 mm); flow, 1 mL/min; eluent A, 0.05% <t>TFA</t> in H 2 O milliQ; eluent B, 0.05% TFA in CH 3 CN; injection, 200 μL; gradient, from 0 to 30% of B in 60 min; λ = 214 nm.
    Deuterated Trifluoracetic Acid Tfa D, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    deuterated trifluoracetic acid tfa d - by Bioz Stars, 2020-03
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    83
    Merck KGaA trifluroacetic acid tfa
    Enzymatic degradation . Analytical <t>HPLC</t> chromatograms of HVP (a), 2HVP (b), DHVP (c) and D-2HVP (d), in 10% FBS at 37 °C after 5 h. Dotted lines indicate the retention time of each peptide in water at the same conditions of analysis. Other peaks in the chromatograms are due to serum components. Conditions: Column, Jupiter C 18 “proteo” (4 μm, 90 Å, 4.6 × 250 mm); flow, 1 mL/min; eluent A, 0.05% <t>TFA</t> in H 2 O milliQ; eluent B, 0.05% TFA in CH 3 CN; injection, 200 μL; gradient, from 0 to 30% of B in 60 min; λ = 214 nm.
    Trifluroacetic Acid Tfa, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 83/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Roche trifluoracetic acid tfa
    Enzymatic degradation . Analytical <t>HPLC</t> chromatograms of HVP (a), 2HVP (b), DHVP (c) and D-2HVP (d), in 10% FBS at 37 °C after 5 h. Dotted lines indicate the retention time of each peptide in water at the same conditions of analysis. Other peaks in the chromatograms are due to serum components. Conditions: Column, Jupiter C 18 “proteo” (4 μm, 90 Å, 4.6 × 250 mm); flow, 1 mL/min; eluent A, 0.05% <t>TFA</t> in H 2 O milliQ; eluent B, 0.05% TFA in CH 3 CN; injection, 200 μL; gradient, from 0 to 30% of B in 60 min; λ = 214 nm.
    Trifluoracetic Acid Tfa, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    trifluoracetic acid tfa - by Bioz Stars, 2020-03
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    79
    Avantor trifluoracetic acid tfa
    Effect of adding DTT to the <t>R4</t> reagent (25% <t>TFA)</t> on the PTH-Cys elution profile in the test peptide.
    Trifluoracetic Acid Tfa, supplied by Avantor, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Mallinckrodt trifluoracetic acid tfa
    Effect of adding DTT to the <t>R4</t> reagent (25% <t>TFA)</t> on the PTH-Cys elution profile in the test peptide.
    Trifluoracetic Acid Tfa, supplied by Mallinckrodt, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Fisher Scientific tfa
    Synthetic strategy for the preparation of photoreactive peptide 1 by on resin fragment condensation. (a) 20% piperidine/DMF; (b) Fmoc-aa-OH, HBTU, HOBt, <t>DIPEA,</t> DMF (2×); (c) Ac 2 O, DIPEA, DMF; (d) 7 , TBTU, HOBt, NMM, DMF; (e) <t>TFA/H</t> 2 O/TIS 95: 2.5:
    Tfa, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 99/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tfa - by Bioz Stars, 2020-03
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    99
    Merck & Co tfa
    Synthetic strategy for the preparation of photoreactive peptide 1 by on resin fragment condensation. (a) 20% piperidine/DMF; (b) Fmoc-aa-OH, HBTU, HOBt, <t>DIPEA,</t> DMF (2×); (c) Ac 2 O, DIPEA, DMF; (d) 7 , TBTU, HOBt, NMM, DMF; (e) <t>TFA/H</t> 2 O/TIS 95: 2.5:
    Tfa, supplied by Merck & Co, used in various techniques. Bioz Stars score: 99/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tfa - by Bioz Stars, 2020-03
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    78
    Millipore trifluroacetic acid tfa hplc grade
    Synthetic strategy for the preparation of photoreactive peptide 1 by on resin fragment condensation. (a) 20% piperidine/DMF; (b) Fmoc-aa-OH, HBTU, HOBt, <t>DIPEA,</t> DMF (2×); (c) Ac 2 O, DIPEA, DMF; (d) 7 , TBTU, HOBt, NMM, DMF; (e) <t>TFA/H</t> 2 O/TIS 95: 2.5:
    Trifluroacetic Acid Tfa Hplc Grade, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Burdick & Jackson tfa
    Synthetic strategy for the preparation of photoreactive peptide 1 by on resin fragment condensation. (a) 20% piperidine/DMF; (b) Fmoc-aa-OH, HBTU, HOBt, <t>DIPEA,</t> DMF (2×); (c) Ac 2 O, DIPEA, DMF; (d) 7 , TBTU, HOBt, NMM, DMF; (e) <t>TFA/H</t> 2 O/TIS 95: 2.5:
    Tfa, supplied by Burdick & Jackson, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tfa - by Bioz Stars, 2020-03
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    78
    Agilent technologies trifluoracetic acid
    Synthetic strategy for the preparation of photoreactive peptide 1 by on resin fragment condensation. (a) 20% piperidine/DMF; (b) Fmoc-aa-OH, HBTU, HOBt, <t>DIPEA,</t> DMF (2×); (c) Ac 2 O, DIPEA, DMF; (d) 7 , TBTU, HOBt, NMM, DMF; (e) <t>TFA/H</t> 2 O/TIS 95: 2.5:
    Trifluoracetic Acid, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Carl Roth GmbH trifluoracetic acid
    Synthetic strategy for the preparation of photoreactive peptide 1 by on resin fragment condensation. (a) 20% piperidine/DMF; (b) Fmoc-aa-OH, HBTU, HOBt, <t>DIPEA,</t> DMF (2×); (c) Ac 2 O, DIPEA, DMF; (d) 7 , TBTU, HOBt, NMM, DMF; (e) <t>TFA/H</t> 2 O/TIS 95: 2.5:
    Trifluoracetic Acid, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 1 article reviews
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    trifluoracetic acid - by Bioz Stars, 2020-03
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    78
    Applichem trifluoracetic acid
    Synthetic strategy for the preparation of photoreactive peptide 1 by on resin fragment condensation. (a) 20% piperidine/DMF; (b) Fmoc-aa-OH, HBTU, HOBt, <t>DIPEA,</t> DMF (2×); (c) Ac 2 O, DIPEA, DMF; (d) 7 , TBTU, HOBt, NMM, DMF; (e) <t>TFA/H</t> 2 O/TIS 95: 2.5:
    Trifluoracetic Acid, supplied by Applichem, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Agilent technologies acetonitrile trifluroacetic acid water
    Synthetic strategy for the preparation of photoreactive peptide 1 by on resin fragment condensation. (a) 20% piperidine/DMF; (b) Fmoc-aa-OH, HBTU, HOBt, <t>DIPEA,</t> DMF (2×); (c) Ac 2 O, DIPEA, DMF; (d) 7 , TBTU, HOBt, NMM, DMF; (e) <t>TFA/H</t> 2 O/TIS 95: 2.5:
    Acetonitrile Trifluroacetic Acid Water, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 16 article reviews
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    99
    Millipore tfa solution
    Synthetic strategy for the preparation of photoreactive peptide 1 by on resin fragment condensation. (a) 20% piperidine/DMF; (b) Fmoc-aa-OH, HBTU, HOBt, <t>DIPEA,</t> DMF (2×); (c) Ac 2 O, DIPEA, DMF; (d) 7 , TBTU, HOBt, NMM, DMF; (e) <t>TFA/H</t> 2 O/TIS 95: 2.5:
    Tfa Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tfa solution/product/Millipore
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    80
    Agilent technologies acn trifluroacetic acid water
    Synthetic strategy for the preparation of photoreactive peptide 1 by on resin fragment condensation. (a) 20% piperidine/DMF; (b) Fmoc-aa-OH, HBTU, HOBt, <t>DIPEA,</t> DMF (2×); (c) Ac 2 O, DIPEA, DMF; (d) 7 , TBTU, HOBt, NMM, DMF; (e) <t>TFA/H</t> 2 O/TIS 95: 2.5:
    Acn Trifluroacetic Acid Water, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Merck KGaA trifluoracetic acid anhydride
    Synthetic strategy for the preparation of photoreactive peptide 1 by on resin fragment condensation. (a) 20% piperidine/DMF; (b) Fmoc-aa-OH, HBTU, HOBt, <t>DIPEA,</t> DMF (2×); (c) Ac 2 O, DIPEA, DMF; (d) 7 , TBTU, HOBt, NMM, DMF; (e) <t>TFA/H</t> 2 O/TIS 95: 2.5:
    Trifluoracetic Acid Anhydride, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Mine Safety Appliances tfa tfa msa
    Immunization of C57BL/6J mice with <t>TFA-MSA</t> and the combined CD40/TLR agonists did not generate TFA-specific immune responses
    Tfa Tfa Msa, supplied by Mine Safety Appliances, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore trifluroacetic acid thf
    Immunization of C57BL/6J mice with <t>TFA-MSA</t> and the combined CD40/TLR agonists did not generate TFA-specific immune responses
    Trifluroacetic Acid Thf, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Cambridge Isotope Laboratories tfa d
    1 Hα, 13 Cα and 13 Cβ chemical shift data of <t>SET-M33</t> in H 2 O/D 2 O (9:1) with addition of TFA- d , pH 4.9 at 298 K. ( A ) CSI values for 1 Hα, 13 Cα and 13 Cβ atoms. ( B ) The absolute difference between observed chemical shift values and random coil chemical shift values for 1 Hα atoms. ( C ) The absolute difference between observed chemical shift values and random coil chemical shift values for 13 Cα and 13 Cβ atoms.
    Tfa D, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore d tfa
    1 Hα, 13 Cα and 13 Cβ chemical shift data of <t>SET-M33</t> in H 2 O/D 2 O (9:1) with addition of TFA- d , pH 4.9 at 298 K. ( A ) CSI values for 1 Hα, 13 Cα and 13 Cβ atoms. ( B ) The absolute difference between observed chemical shift values and random coil chemical shift values for 1 Hα atoms. ( C ) The absolute difference between observed chemical shift values and random coil chemical shift values for 13 Cα and 13 Cβ atoms.
    D Tfa, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    New England Peptide tfa salts
    1 Hα, 13 Cα and 13 Cβ chemical shift data of <t>SET-M33</t> in H 2 O/D 2 O (9:1) with addition of TFA- d , pH 4.9 at 298 K. ( A ) CSI values for 1 Hα, 13 Cα and 13 Cβ atoms. ( B ) The absolute difference between observed chemical shift values and random coil chemical shift values for 1 Hα atoms. ( C ) The absolute difference between observed chemical shift values and random coil chemical shift values for 13 Cα and 13 Cβ atoms.
    Tfa Salts, supplied by New England Peptide, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Capillary HILIC-MS of an E. coli lysate (5 μL loaded of a 2.5 mg/mL solution in 2% ACN and 0.1% TFA). (top) Base-peak chromatogram (900–3000 m / z ); (bottom) feature map showing deconvoluted MS spectra. The deconvolution algorithm is based on isotopically resolved molecular features, and therefore, potential features above 35 kDa are not identified. Mobile phases as specified in the Experimental Section . Loading at 10% B, multisegment linear gradient from 10–12% B in 1 min, 12–30% in 30 min, 30–65% B in 6 min, 65–90% B in 1 min, followed by 3 min at 90% B and several washing steps (total analysis time of 60 min).

    Journal: Analytical Chemistry

    Article Title: Capillary HILIC-MS: A New Tool for Sensitive Top-Down Proteomics

    doi: 10.1021/acs.analchem.8b00382

    Figure Lengend Snippet: Capillary HILIC-MS of an E. coli lysate (5 μL loaded of a 2.5 mg/mL solution in 2% ACN and 0.1% TFA). (top) Base-peak chromatogram (900–3000 m / z ); (bottom) feature map showing deconvoluted MS spectra. The deconvolution algorithm is based on isotopically resolved molecular features, and therefore, potential features above 35 kDa are not identified. Mobile phases as specified in the Experimental Section . Loading at 10% B, multisegment linear gradient from 10–12% B in 1 min, 12–30% in 30 min, 30–65% B in 6 min, 65–90% B in 1 min, followed by 3 min at 90% B and several washing steps (total analysis time of 60 min).

    Article Snippet: MS grade acetonitrile (ACN) and TFA were purchased from Biosolve B.V. (Valkenswaard, The Netherlands).

    Techniques: Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry

    Enzymatic degradation . Analytical HPLC chromatograms of HVP (a), 2HVP (b), DHVP (c) and D-2HVP (d), in 10% FBS at 37 °C after 5 h. Dotted lines indicate the retention time of each peptide in water at the same conditions of analysis. Other peaks in the chromatograms are due to serum components. Conditions: Column, Jupiter C 18 “proteo” (4 μm, 90 Å, 4.6 × 250 mm); flow, 1 mL/min; eluent A, 0.05% TFA in H 2 O milliQ; eluent B, 0.05% TFA in CH 3 CN; injection, 200 μL; gradient, from 0 to 30% of B in 60 min; λ = 214 nm.

    Journal: Bioactive Materials

    Article Title: Smart biomaterials: Surfaces functionalized with proteolytically stable osteoblast-adhesive peptides

    doi: 10.1016/j.bioactmat.2017.05.004

    Figure Lengend Snippet: Enzymatic degradation . Analytical HPLC chromatograms of HVP (a), 2HVP (b), DHVP (c) and D-2HVP (d), in 10% FBS at 37 °C after 5 h. Dotted lines indicate the retention time of each peptide in water at the same conditions of analysis. Other peaks in the chromatograms are due to serum components. Conditions: Column, Jupiter C 18 “proteo” (4 μm, 90 Å, 4.6 × 250 mm); flow, 1 mL/min; eluent A, 0.05% TFA in H 2 O milliQ; eluent B, 0.05% TFA in CH 3 CN; injection, 200 μL; gradient, from 0 to 30% of B in 60 min; λ = 214 nm.

    Article Snippet: Solvents for chromatography such as acetonitrile and TFA (HPLC grade) were from Sigma-Aldrich.

    Techniques: High Performance Liquid Chromatography, Flow Cytometry, Injection

    Effect of adding DTT to the R4 reagent (25% TFA) on the PTH-Cys elution profile in the test peptide.

    Journal:

    Article Title: ABRF ESRG 2005 Study: Identification of Seven Modified Amino Acids by Edman Sequencing

    doi:

    Figure Lengend Snippet: Effect of adding DTT to the R4 reagent (25% TFA) on the PTH-Cys elution profile in the test peptide.

    Article Snippet: Reagents used for Edman degradation sequencing by the research committee member laboratories were from Applied Biosystems, Inc. (ABI), except for those used in the Porton sequencer, which were as follows: R1, 5% PITC (ABI) diluted to 2% with heptane (Burdick & Jackson sequencing grade); R2, 2 mL diisopropylethyl-amine (Aldrich, double distilled) in 125 mL nanopure water; R3 and R4, 100% TFA and 25% TFA, respectively (JT Baker, high-performance liquid chromatography [HPLC] grade) in nanopure water; S1, 2.5–3.5 mg DTT (Research Organics) in 150 mL ethyl acetate (EMD Omnisolv) that had been treated with neutral alumina (100 g/L, Fluka type 507C) and filtered through a 0.2-μm nylon filter; and S2, 10% acetonitrile (Fisher Optima) in nanopure water.

    Techniques:

    Synthetic strategy for the preparation of photoreactive peptide 1 by on resin fragment condensation. (a) 20% piperidine/DMF; (b) Fmoc-aa-OH, HBTU, HOBt, DIPEA, DMF (2×); (c) Ac 2 O, DIPEA, DMF; (d) 7 , TBTU, HOBt, NMM, DMF; (e) TFA/H 2 O/TIS 95: 2.5:

    Journal: Organic & biomolecular chemistry

    Article Title: Synthesis and characterization of a photocleavable collagen-like peptide

    doi: 10.1039/c7ob02198d

    Figure Lengend Snippet: Synthetic strategy for the preparation of photoreactive peptide 1 by on resin fragment condensation. (a) 20% piperidine/DMF; (b) Fmoc-aa-OH, HBTU, HOBt, DIPEA, DMF (2×); (c) Ac 2 O, DIPEA, DMF; (d) 7 , TBTU, HOBt, NMM, DMF; (e) TFA/H 2 O/TIS 95: 2.5:

    Article Snippet: Solvents, DIPEA, TFA, and bromophenol blue were obtained from Fisher Scientific.

    Techniques:

    SPPS of the protected photoreactive peptide 7 on diphenyl-diazomethane resin. (a) 6 , DCM/DMF 2: 1; (b) HOAc/DCM; (c) 20% piperidine/DMF; (d) Fmoc-aa-OH, HBTU, HOBt, DIPEA, DMF (2×); (e) Ac 2 O, DIPEA, DMF; (f ) 3% TFA/DCM.

    Journal: Organic & biomolecular chemistry

    Article Title: Synthesis and characterization of a photocleavable collagen-like peptide

    doi: 10.1039/c7ob02198d

    Figure Lengend Snippet: SPPS of the protected photoreactive peptide 7 on diphenyl-diazomethane resin. (a) 6 , DCM/DMF 2: 1; (b) HOAc/DCM; (c) 20% piperidine/DMF; (d) Fmoc-aa-OH, HBTU, HOBt, DIPEA, DMF (2×); (e) Ac 2 O, DIPEA, DMF; (f ) 3% TFA/DCM.

    Article Snippet: Solvents, DIPEA, TFA, and bromophenol blue were obtained from Fisher Scientific.

    Techniques:

    Immunization of C57BL/6J mice with TFA-MSA and the combined CD40/TLR agonists did not generate TFA-specific immune responses

    Journal: Toxicology letters

    Article Title: Generation of T Cell Responses Targeting the Reactive Metabolite of Halothane in Mice

    doi: 10.1016/j.toxlet.2010.02.009

    Figure Lengend Snippet: Immunization of C57BL/6J mice with TFA-MSA and the combined CD40/TLR agonists did not generate TFA-specific immune responses

    Article Snippet: In the present study, we adopted the combined CD40/TLR agonists approach and demonstrated that immunization of mice with mouse serum albumin adducts of TFA (TFA-MSA) in the presence of the combined agonists could elicit CD4+ and CD8+ T cell responses systemically as well as locally within the liver.

    Techniques: Mouse Assay

    Immunization of BALB/cByJ mice with TFA-MSA and the combined CD40/TLR agonist generated strong TFA-specific immune responses

    Journal: Toxicology letters

    Article Title: Generation of T Cell Responses Targeting the Reactive Metabolite of Halothane in Mice

    doi: 10.1016/j.toxlet.2010.02.009

    Figure Lengend Snippet: Immunization of BALB/cByJ mice with TFA-MSA and the combined CD40/TLR agonist generated strong TFA-specific immune responses

    Article Snippet: In the present study, we adopted the combined CD40/TLR agonists approach and demonstrated that immunization of mice with mouse serum albumin adducts of TFA (TFA-MSA) in the presence of the combined agonists could elicit CD4+ and CD8+ T cell responses systemically as well as locally within the liver.

    Techniques: Mouse Assay, Generated

    Both CD4 + and CD8 + subsets of TFA-specific T cells were generated in BALB/cByJ mice immunized by TFA-MSA and the combined CD40/CpG agonist

    Journal: Toxicology letters

    Article Title: Generation of T Cell Responses Targeting the Reactive Metabolite of Halothane in Mice

    doi: 10.1016/j.toxlet.2010.02.009

    Figure Lengend Snippet: Both CD4 + and CD8 + subsets of TFA-specific T cells were generated in BALB/cByJ mice immunized by TFA-MSA and the combined CD40/CpG agonist

    Article Snippet: In the present study, we adopted the combined CD40/TLR agonists approach and demonstrated that immunization of mice with mouse serum albumin adducts of TFA (TFA-MSA) in the presence of the combined agonists could elicit CD4+ and CD8+ T cell responses systemically as well as locally within the liver.

    Techniques: Generated, Mouse Assay

    Immunization of BALB/cByJ mice with TFA-MSA and the combined CD40/TLR9 agonist generated TFA-specific T cells in the liver

    Journal: Toxicology letters

    Article Title: Generation of T Cell Responses Targeting the Reactive Metabolite of Halothane in Mice

    doi: 10.1016/j.toxlet.2010.02.009

    Figure Lengend Snippet: Immunization of BALB/cByJ mice with TFA-MSA and the combined CD40/TLR9 agonist generated TFA-specific T cells in the liver

    Article Snippet: In the present study, we adopted the combined CD40/TLR agonists approach and demonstrated that immunization of mice with mouse serum albumin adducts of TFA (TFA-MSA) in the presence of the combined agonists could elicit CD4+ and CD8+ T cell responses systemically as well as locally within the liver.

    Techniques: Mouse Assay, Generated

    Measurement of TFA-specific immune responses in BALB/cByJ mice immunized by TFA-MSA in conjunction with CFA

    Journal: Toxicology letters

    Article Title: Generation of T Cell Responses Targeting the Reactive Metabolite of Halothane in Mice

    doi: 10.1016/j.toxlet.2010.02.009

    Figure Lengend Snippet: Measurement of TFA-specific immune responses in BALB/cByJ mice immunized by TFA-MSA in conjunction with CFA

    Article Snippet: In the present study, we adopted the combined CD40/TLR agonists approach and demonstrated that immunization of mice with mouse serum albumin adducts of TFA (TFA-MSA) in the presence of the combined agonists could elicit CD4+ and CD8+ T cell responses systemically as well as locally within the liver.

    Techniques: Mouse Assay

    Immunization of DBA/1 mice with TFA-MSA in combination with CD40/polyI:C, but not CD40/LPS or CD40/CpG generated TFA-specific immune responses

    Journal: Toxicology letters

    Article Title: Generation of T Cell Responses Targeting the Reactive Metabolite of Halothane in Mice

    doi: 10.1016/j.toxlet.2010.02.009

    Figure Lengend Snippet: Immunization of DBA/1 mice with TFA-MSA in combination with CD40/polyI:C, but not CD40/LPS or CD40/CpG generated TFA-specific immune responses

    Article Snippet: In the present study, we adopted the combined CD40/TLR agonists approach and demonstrated that immunization of mice with mouse serum albumin adducts of TFA (TFA-MSA) in the presence of the combined agonists could elicit CD4+ and CD8+ T cell responses systemically as well as locally within the liver.

    Techniques: Mouse Assay, Generated

    1 Hα, 13 Cα and 13 Cβ chemical shift data of SET-M33 in H 2 O/D 2 O (9:1) with addition of TFA- d , pH 4.9 at 298 K. ( A ) CSI values for 1 Hα, 13 Cα and 13 Cβ atoms. ( B ) The absolute difference between observed chemical shift values and random coil chemical shift values for 1 Hα atoms. ( C ) The absolute difference between observed chemical shift values and random coil chemical shift values for 13 Cα and 13 Cβ atoms.

    Journal: Molecules

    Article Title: NMR Study of the Secondary Structure and Biopharmaceutical Formulation of an Active Branched Antimicrobial Peptide

    doi: 10.3390/molecules24234290

    Figure Lengend Snippet: 1 Hα, 13 Cα and 13 Cβ chemical shift data of SET-M33 in H 2 O/D 2 O (9:1) with addition of TFA- d , pH 4.9 at 298 K. ( A ) CSI values for 1 Hα, 13 Cα and 13 Cβ atoms. ( B ) The absolute difference between observed chemical shift values and random coil chemical shift values for 1 Hα atoms. ( C ) The absolute difference between observed chemical shift values and random coil chemical shift values for 13 Cα and 13 Cβ atoms.

    Article Snippet: All spectra were recorded at 298 K with 12.5 mg/mL SET-M33 dissolved in H2 O/D2 O 9:1 with 0.5 μL TFA-d (99.5%D; Cambridge Isotope Laboratories, Tewksbury, MA, USA) with 5 μL 10 mM DSS (4,4-dimethyl-4-silapentane-1-sulfonic acid, 97%, Cambridge Isotope Laboratories) in D2 O added as the internal chemical shift calibration standard.

    Techniques:

    1D 1 H-NMR spectrum of 12.5 mg/mL SET-M33 in H 2 O/D 2 O (9:1) and TFA- d (12.5 mg/mL) at 298 K.

    Journal: Molecules

    Article Title: NMR Study of the Secondary Structure and Biopharmaceutical Formulation of an Active Branched Antimicrobial Peptide

    doi: 10.3390/molecules24234290

    Figure Lengend Snippet: 1D 1 H-NMR spectrum of 12.5 mg/mL SET-M33 in H 2 O/D 2 O (9:1) and TFA- d (12.5 mg/mL) at 298 K.

    Article Snippet: All spectra were recorded at 298 K with 12.5 mg/mL SET-M33 dissolved in H2 O/D2 O 9:1 with 0.5 μL TFA-d (99.5%D; Cambridge Isotope Laboratories, Tewksbury, MA, USA) with 5 μL 10 mM DSS (4,4-dimethyl-4-silapentane-1-sulfonic acid, 97%, Cambridge Isotope Laboratories) in D2 O added as the internal chemical shift calibration standard.

    Techniques: Nuclear Magnetic Resonance

    ( A ) The primary structure of SET-M33 with amino acid numbering. Amino acids are labeled with a three-letter code and sequence number as reported above. ( B ) The 1 HN- 1 Hα fingerprint region of the 2D 1 H COSY spectrum of SET-M33 in H 2 O/D 2 O (9:1) with addition of TFA- d , pH 4.9 at 298 K. The assignment of each cross-peak is shown using the three-letter amino acid code followed by the sequence number. ( C ) The 2D 1 H- 15 N HSQC spectrum of SET-M33 in H 2 O/D 2 O (9:1) with addition of TFA- d at 298 K. The peaks for the sidechain εNH groups of Arg4,6 are folded into the spectrum with opposite sign (blue color). The assignment of each cross-peak is indicated using the three-letter amino acid code followed by the sequence number.

    Journal: Molecules

    Article Title: NMR Study of the Secondary Structure and Biopharmaceutical Formulation of an Active Branched Antimicrobial Peptide

    doi: 10.3390/molecules24234290

    Figure Lengend Snippet: ( A ) The primary structure of SET-M33 with amino acid numbering. Amino acids are labeled with a three-letter code and sequence number as reported above. ( B ) The 1 HN- 1 Hα fingerprint region of the 2D 1 H COSY spectrum of SET-M33 in H 2 O/D 2 O (9:1) with addition of TFA- d , pH 4.9 at 298 K. The assignment of each cross-peak is shown using the three-letter amino acid code followed by the sequence number. ( C ) The 2D 1 H- 15 N HSQC spectrum of SET-M33 in H 2 O/D 2 O (9:1) with addition of TFA- d at 298 K. The peaks for the sidechain εNH groups of Arg4,6 are folded into the spectrum with opposite sign (blue color). The assignment of each cross-peak is indicated using the three-letter amino acid code followed by the sequence number.

    Article Snippet: All spectra were recorded at 298 K with 12.5 mg/mL SET-M33 dissolved in H2 O/D2 O 9:1 with 0.5 μL TFA-d (99.5%D; Cambridge Isotope Laboratories, Tewksbury, MA, USA) with 5 μL 10 mM DSS (4,4-dimethyl-4-silapentane-1-sulfonic acid, 97%, Cambridge Isotope Laboratories) in D2 O added as the internal chemical shift calibration standard.

    Techniques: Labeling, Sequencing

    Sequential walk through the amide backbone fingerprint region of 2D 1 H NOESY spectrum of SET-M33 in H 2 O/D 2 O (9:1) with addition of TFA- d , pH 4.9 298 K is shown. The assignment of each cross-peak is shown using the three-letter amino acid code followed by the sequence number. The sequential walk follows the arrows between alternating sequential inter-residue HN(i)-Hα(i-1) and intra-residue HN(i)-Hα(i) NOEs.

    Journal: Molecules

    Article Title: NMR Study of the Secondary Structure and Biopharmaceutical Formulation of an Active Branched Antimicrobial Peptide

    doi: 10.3390/molecules24234290

    Figure Lengend Snippet: Sequential walk through the amide backbone fingerprint region of 2D 1 H NOESY spectrum of SET-M33 in H 2 O/D 2 O (9:1) with addition of TFA- d , pH 4.9 298 K is shown. The assignment of each cross-peak is shown using the three-letter amino acid code followed by the sequence number. The sequential walk follows the arrows between alternating sequential inter-residue HN(i)-Hα(i-1) and intra-residue HN(i)-Hα(i) NOEs.

    Article Snippet: All spectra were recorded at 298 K with 12.5 mg/mL SET-M33 dissolved in H2 O/D2 O 9:1 with 0.5 μL TFA-d (99.5%D; Cambridge Isotope Laboratories, Tewksbury, MA, USA) with 5 μL 10 mM DSS (4,4-dimethyl-4-silapentane-1-sulfonic acid, 97%, Cambridge Isotope Laboratories) in D2 O added as the internal chemical shift calibration standard.

    Techniques: Sequencing