Journal: EJNMMI Research
Article Title: Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake
Figure Lengend Snippet: PET images, summed 30–60 min, and TACs from a Balbc nu/nu mouse (prone) bearing tumors ( white arrows ): a one A431 xenograft (1 × 10 7 cells, 15 days) or b two A431 xenografts ( left : 1 × 10 7 cells, 28 days; right : 1 × 10 7 cells, 25 days). Comparison A shows a 7-times higher uptake with targeting [methyl- 11 C]-Z EGFR:2377 -ST-CH 3 compared to the non-targeting [methyl- 11 C]-Z Taq:3638 -ST-CH 3 . Comparison B illustrates uptake of the targeting Affibody increasing as the tumors grow from time from inoculation
Article Snippet: Preparation and labeling of the Sel-tagged Affibody molecules The Sel-tagged Affibody molecules were successfully obtained by expression in E. coli as C-terminal fusions to green fluorescent protein (GFP), then recovered with immobilized metal ion affinity chromatography (IMAC), released by tobacco etch virus (TEV)-protease cleavage, and purified by high-performance liquid chromatography (HPLC).
Techniques: Positron Emission Tomography