tet on 3g inducible expression system Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • prev tet on  (TaKaRa)
    94
    TaKaRa prev tet on
    Prev Tet On, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prev tet on/product/TaKaRa
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    prev tet on - by Bioz Stars, 2023-02
    94/100 stars
    		  Buy from Supplier
    tet on 3g  (TaKaRa)
    95
    TaKaRa tet on 3g
    Tet On 3g, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tet on 3g/product/TaKaRa
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tet on 3g - by Bioz Stars, 2023-02
    95/100 stars
    		  Buy from Supplier
    ptre3g bi  (TaKaRa)
    94
    TaKaRa ptre3g bi
    The Notch signaling pathway was upregulated after hepatocellular carcinoma induction, and the inhibition of Notch signaling suppressed hepatocyte dedifferentiation. ( A ) WISH results showed the expression pattern of notch1a , notch1b , notch2 , notch3 , and her15 at 10 dpf after DOX activation of kras G12V . ( B ) Tg(fabp10a:Tet3G;TRE3G:kras G12V <t>-ZsGreen1)</t> with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. ( C ) Monolayer images of Anxa4 antibody staining showed expression changes in the DOX-induced kras G12V + and kras G12V + and dnMAML+ groups at 8 dpf and 10 dpf. ( D ) Statistics of Anxa4 + and ZsGreen1 + /ZsGreen1+ ratio in the DOX-induced kras G12V + ( n = 11) and kras G12V + and dnMAML+ ( n = 13) groups at 8 dpf and 10 dpf. ( E ) The results of fluorescent in situ hybridization (FISH) showed sox9b expression in the DOX-induced control and kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. ( F ) The results of FISH showed cp expression in the DOX-induced control, kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. Numbers indicate the proportion of larvae exhibiting that expression. Asterisks show significance: ****— p < 0.0001. Scale bars—100 μm; error bars—S.D.
    Ptre3g Bi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptre3g bi/product/TaKaRa
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ptre3g bi - by Bioz Stars, 2023-02
    94/100 stars
    		  Buy from Supplier
    lentiviral tet on system  (TaKaRa)
    94
    TaKaRa lentiviral tet on system
    The Notch signaling pathway was upregulated after hepatocellular carcinoma induction, and the inhibition of Notch signaling suppressed hepatocyte dedifferentiation. ( A ) WISH results showed the expression pattern of notch1a , notch1b , notch2 , notch3 , and her15 at 10 dpf after DOX activation of kras G12V . ( B ) Tg(fabp10a:Tet3G;TRE3G:kras G12V <t>-ZsGreen1)</t> with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. ( C ) Monolayer images of Anxa4 antibody staining showed expression changes in the DOX-induced kras G12V + and kras G12V + and dnMAML+ groups at 8 dpf and 10 dpf. ( D ) Statistics of Anxa4 + and ZsGreen1 + /ZsGreen1+ ratio in the DOX-induced kras G12V + ( n = 11) and kras G12V + and dnMAML+ ( n = 13) groups at 8 dpf and 10 dpf. ( E ) The results of fluorescent in situ hybridization (FISH) showed sox9b expression in the DOX-induced control and kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. ( F ) The results of FISH showed cp expression in the DOX-induced control, kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. Numbers indicate the proportion of larvae exhibiting that expression. Asterisks show significance: ****— p < 0.0001. Scale bars—100 μm; error bars—S.D.
    Lentiviral Tet On System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lentiviral tet on system/product/TaKaRa
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lentiviral tet on system - by Bioz Stars, 2023-02
    94/100 stars
    		  Buy from Supplier
    retro x tet on 3g inducible expression system  (TaKaRa)
    94
    TaKaRa retro x tet on 3g inducible expression system
    The Notch signaling pathway was upregulated after hepatocellular carcinoma induction, and the inhibition of Notch signaling suppressed hepatocyte dedifferentiation. ( A ) WISH results showed the expression pattern of notch1a , notch1b , notch2 , notch3 , and her15 at 10 dpf after DOX activation of kras G12V . ( B ) Tg(fabp10a:Tet3G;TRE3G:kras G12V <t>-ZsGreen1)</t> with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. ( C ) Monolayer images of Anxa4 antibody staining showed expression changes in the DOX-induced kras G12V + and kras G12V + and dnMAML+ groups at 8 dpf and 10 dpf. ( D ) Statistics of Anxa4 + and ZsGreen1 + /ZsGreen1+ ratio in the DOX-induced kras G12V + ( n = 11) and kras G12V + and dnMAML+ ( n = 13) groups at 8 dpf and 10 dpf. ( E ) The results of fluorescent in situ hybridization (FISH) showed sox9b expression in the DOX-induced control and kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. ( F ) The results of FISH showed cp expression in the DOX-induced control, kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. Numbers indicate the proportion of larvae exhibiting that expression. Asterisks show significance: ****— p < 0.0001. Scale bars—100 μm; error bars—S.D.
    Retro X Tet On 3g Inducible Expression System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/retro x tet on 3g inducible expression system/product/TaKaRa
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    retro x tet on 3g inducible expression system - by Bioz Stars, 2023-02
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    The Notch signaling pathway was upregulated after hepatocellular carcinoma induction, and the inhibition of Notch signaling suppressed hepatocyte dedifferentiation. ( A ) WISH results showed the expression pattern of notch1a , notch1b , notch2 , notch3 , and her15 at 10 dpf after DOX activation of kras G12V . ( B ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. ( C ) Monolayer images of Anxa4 antibody staining showed expression changes in the DOX-induced kras G12V + and kras G12V + and dnMAML+ groups at 8 dpf and 10 dpf. ( D ) Statistics of Anxa4 + and ZsGreen1 + /ZsGreen1+ ratio in the DOX-induced kras G12V + ( n = 11) and kras G12V + and dnMAML+ ( n = 13) groups at 8 dpf and 10 dpf. ( E ) The results of fluorescent in situ hybridization (FISH) showed sox9b expression in the DOX-induced control and kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. ( F ) The results of FISH showed cp expression in the DOX-induced control, kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. Numbers indicate the proportion of larvae exhibiting that expression. Asterisks show significance: ****— p < 0.0001. Scale bars—100 μm; error bars—S.D.

    Journal: International Journal of Molecular Sciences

    Article Title: Notch–Sox9 Axis Mediates Hepatocyte Dedifferentiation in Kras G12V -Induced Zebrafish Hepatocellular Carcinoma

    doi: 10.3390/ijms23094705

    Figure Lengend Snippet: The Notch signaling pathway was upregulated after hepatocellular carcinoma induction, and the inhibition of Notch signaling suppressed hepatocyte dedifferentiation. ( A ) WISH results showed the expression pattern of notch1a , notch1b , notch2 , notch3 , and her15 at 10 dpf after DOX activation of kras G12V . ( B ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. ( C ) Monolayer images of Anxa4 antibody staining showed expression changes in the DOX-induced kras G12V + and kras G12V + and dnMAML+ groups at 8 dpf and 10 dpf. ( D ) Statistics of Anxa4 + and ZsGreen1 + /ZsGreen1+ ratio in the DOX-induced kras G12V + ( n = 11) and kras G12V + and dnMAML+ ( n = 13) groups at 8 dpf and 10 dpf. ( E ) The results of fluorescent in situ hybridization (FISH) showed sox9b expression in the DOX-induced control and kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. ( F ) The results of FISH showed cp expression in the DOX-induced control, kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. Numbers indicate the proportion of larvae exhibiting that expression. Asterisks show significance: ****— p < 0.0001. Scale bars—100 μm; error bars—S.D.

    Article Snippet: The cDNA of full-length Zebrafish kras was amplified by PrimeSTAR HS DNA polymerase (Takara) and point mutated to obtain kras G12V , cloned into the modified pTRE3G-BI:ZsGreen1 (Cat.631342, Clontech) vector; and TRE3G-BI is a bidirectional version of TRE3G promoter that allows for simultaneous, equivalent, and inducible expression of two transgenes.

    Techniques: Inhibition, Expressing, Activation Assay, Transgenic Assay, Staining, In Situ Hybridization

    Hepatic Sox9 expression is upregulated after hepatocellular carcinoma induction, and the sox9b fh313 mutant suppressed hepatocyte dedifferentiation. ( A ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. ( B , C ) Sox9 antibody staining indicated the number of Sox9+ cells in the liver at 10 dpf in the DOX-induced control ( n = 5), kras G12V + ( n = 5) and kras G12V + and dnMAML+ ( n = 5) groups with statistical results. ( D ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) treatment strategy in sox9b fh313 mutant. ( E ) Three-dimensional images of Anxa4 antibody staining showed biliary duct changes in the DOX-induced kras G12V + and kras G12V + sox9b fh313 mutants at 8 dpf and 10 dpf. ( F ) Monolayer images of Anxa4 antibody staining biliary duct changes in the DOX-induced kras G12V + and kras G12V + sox9b fh313 mutant at 8 dpf and 10 dpf. Asterisks show significance: *— p < 0.05; **— p < 0.01. Scale bars—100 μm; error bars—S.D.

    Journal: International Journal of Molecular Sciences

    Article Title: Notch–Sox9 Axis Mediates Hepatocyte Dedifferentiation in Kras G12V -Induced Zebrafish Hepatocellular Carcinoma

    doi: 10.3390/ijms23094705

    Figure Lengend Snippet: Hepatic Sox9 expression is upregulated after hepatocellular carcinoma induction, and the sox9b fh313 mutant suppressed hepatocyte dedifferentiation. ( A ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. ( B , C ) Sox9 antibody staining indicated the number of Sox9+ cells in the liver at 10 dpf in the DOX-induced control ( n = 5), kras G12V + ( n = 5) and kras G12V + and dnMAML+ ( n = 5) groups with statistical results. ( D ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) treatment strategy in sox9b fh313 mutant. ( E ) Three-dimensional images of Anxa4 antibody staining showed biliary duct changes in the DOX-induced kras G12V + and kras G12V + sox9b fh313 mutants at 8 dpf and 10 dpf. ( F ) Monolayer images of Anxa4 antibody staining biliary duct changes in the DOX-induced kras G12V + and kras G12V + sox9b fh313 mutant at 8 dpf and 10 dpf. Asterisks show significance: *— p < 0.05; **— p < 0.01. Scale bars—100 μm; error bars—S.D.

    Article Snippet: The cDNA of full-length Zebrafish kras was amplified by PrimeSTAR HS DNA polymerase (Takara) and point mutated to obtain kras G12V , cloned into the modified pTRE3G-BI:ZsGreen1 (Cat.631342, Clontech) vector; and TRE3G-BI is a bidirectional version of TRE3G promoter that allows for simultaneous, equivalent, and inducible expression of two transgenes.

    Techniques: Expressing, Mutagenesis, Transgenic Assay, Staining

    Inhibition of the Notch signaling pathway after liver cancer induction reduced cancer cell migration and improved survival. ( A ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy to observe the migration of cancer cells. ( B ) Zebrafish larvae were classified into three classes, I, II, and III, by the number of cancer cells outside the liver and the number of locations of metastases. ( C ) The proportion of the total number of larvae in different metastasis classes in the kras G12V + ( n = 46) and kras G12V + and dnMAML+ ( n = 130) groups at 9 dpf. ( D ) Kaplan–Meier survival curves of the DOX-induced kras G12V + ( n = 191) and kras G12V + and dnMAML+ ( n = 163) groups. p values for survival curves were calculated by log-rank test. Scale bars , 100 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Notch–Sox9 Axis Mediates Hepatocyte Dedifferentiation in Kras G12V -Induced Zebrafish Hepatocellular Carcinoma

    doi: 10.3390/ijms23094705

    Figure Lengend Snippet: Inhibition of the Notch signaling pathway after liver cancer induction reduced cancer cell migration and improved survival. ( A ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy to observe the migration of cancer cells. ( B ) Zebrafish larvae were classified into three classes, I, II, and III, by the number of cancer cells outside the liver and the number of locations of metastases. ( C ) The proportion of the total number of larvae in different metastasis classes in the kras G12V + ( n = 46) and kras G12V + and dnMAML+ ( n = 130) groups at 9 dpf. ( D ) Kaplan–Meier survival curves of the DOX-induced kras G12V + ( n = 191) and kras G12V + and dnMAML+ ( n = 163) groups. p values for survival curves were calculated by log-rank test. Scale bars , 100 μm.

    Article Snippet: The cDNA of full-length Zebrafish kras was amplified by PrimeSTAR HS DNA polymerase (Takara) and point mutated to obtain kras G12V , cloned into the modified pTRE3G-BI:ZsGreen1 (Cat.631342, Clontech) vector; and TRE3G-BI is a bidirectional version of TRE3G promoter that allows for simultaneous, equivalent, and inducible expression of two transgenes.

    Techniques: Inhibition, Migration, Transgenic Assay