tet on 3g inducible expression system Search Results


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  • 94
    TaKaRa prev tet on
    Prev Tet On, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa tet on 3g
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    TaKaRa ptre3g bi
    The Notch signaling pathway was upregulated after hepatocellular carcinoma induction, and the inhibition of Notch signaling suppressed hepatocyte dedifferentiation. ( A ) WISH results showed the expression pattern of notch1a , notch1b , notch2 , notch3 , and her15 at 10 dpf after DOX activation of kras G12V . ( B ) Tg(fabp10a:Tet3G;TRE3G:kras G12V <t>-ZsGreen1)</t> with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. ( C ) Monolayer images of Anxa4 antibody staining showed expression changes in the DOX-induced kras G12V + and kras G12V + and dnMAML+ groups at 8 dpf and 10 dpf. ( D ) Statistics of Anxa4 + and ZsGreen1 + /ZsGreen1+ ratio in the DOX-induced kras G12V + ( n = 11) and kras G12V + and dnMAML+ ( n = 13) groups at 8 dpf and 10 dpf. ( E ) The results of fluorescent in situ hybridization (FISH) showed sox9b expression in the DOX-induced control and kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. ( F ) The results of FISH showed cp expression in the DOX-induced control, kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. Numbers indicate the proportion of larvae exhibiting that expression. Asterisks show significance: ****— p < 0.0001. Scale bars—100 μm; error bars—S.D.
    Ptre3g Bi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa lentiviral tet on system
    The Notch signaling pathway was upregulated after hepatocellular carcinoma induction, and the inhibition of Notch signaling suppressed hepatocyte dedifferentiation. ( A ) WISH results showed the expression pattern of notch1a , notch1b , notch2 , notch3 , and her15 at 10 dpf after DOX activation of kras G12V . ( B ) Tg(fabp10a:Tet3G;TRE3G:kras G12V <t>-ZsGreen1)</t> with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. ( C ) Monolayer images of Anxa4 antibody staining showed expression changes in the DOX-induced kras G12V + and kras G12V + and dnMAML+ groups at 8 dpf and 10 dpf. ( D ) Statistics of Anxa4 + and ZsGreen1 + /ZsGreen1+ ratio in the DOX-induced kras G12V + ( n = 11) and kras G12V + and dnMAML+ ( n = 13) groups at 8 dpf and 10 dpf. ( E ) The results of fluorescent in situ hybridization (FISH) showed sox9b expression in the DOX-induced control and kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. ( F ) The results of FISH showed cp expression in the DOX-induced control, kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. Numbers indicate the proportion of larvae exhibiting that expression. Asterisks show significance: ****— p < 0.0001. Scale bars—100 μm; error bars—S.D.
    Lentiviral Tet On System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa retro x tet on 3g inducible expression system
    The Notch signaling pathway was upregulated after hepatocellular carcinoma induction, and the inhibition of Notch signaling suppressed hepatocyte dedifferentiation. ( A ) WISH results showed the expression pattern of notch1a , notch1b , notch2 , notch3 , and her15 at 10 dpf after DOX activation of kras G12V . ( B ) Tg(fabp10a:Tet3G;TRE3G:kras G12V <t>-ZsGreen1)</t> with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. ( C ) Monolayer images of Anxa4 antibody staining showed expression changes in the DOX-induced kras G12V + and kras G12V + and dnMAML+ groups at 8 dpf and 10 dpf. ( D ) Statistics of Anxa4 + and ZsGreen1 + /ZsGreen1+ ratio in the DOX-induced kras G12V + ( n = 11) and kras G12V + and dnMAML+ ( n = 13) groups at 8 dpf and 10 dpf. ( E ) The results of fluorescent in situ hybridization (FISH) showed sox9b expression in the DOX-induced control and kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. ( F ) The results of FISH showed cp expression in the DOX-induced control, kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. Numbers indicate the proportion of larvae exhibiting that expression. Asterisks show significance: ****— p < 0.0001. Scale bars—100 μm; error bars—S.D.
    Retro X Tet On 3g Inducible Expression System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The Notch signaling pathway was upregulated after hepatocellular carcinoma induction, and the inhibition of Notch signaling suppressed hepatocyte dedifferentiation. ( A ) WISH results showed the expression pattern of notch1a , notch1b , notch2 , notch3 , and her15 at 10 dpf after DOX activation of kras G12V . ( B ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. ( C ) Monolayer images of Anxa4 antibody staining showed expression changes in the DOX-induced kras G12V + and kras G12V + and dnMAML+ groups at 8 dpf and 10 dpf. ( D ) Statistics of Anxa4 + and ZsGreen1 + /ZsGreen1+ ratio in the DOX-induced kras G12V + ( n = 11) and kras G12V + and dnMAML+ ( n = 13) groups at 8 dpf and 10 dpf. ( E ) The results of fluorescent in situ hybridization (FISH) showed sox9b expression in the DOX-induced control and kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. ( F ) The results of FISH showed cp expression in the DOX-induced control, kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. Numbers indicate the proportion of larvae exhibiting that expression. Asterisks show significance: ****— p < 0.0001. Scale bars—100 μm; error bars—S.D.

    Journal: International Journal of Molecular Sciences

    Article Title: Notch–Sox9 Axis Mediates Hepatocyte Dedifferentiation in Kras G12V -Induced Zebrafish Hepatocellular Carcinoma

    doi: 10.3390/ijms23094705

    Figure Lengend Snippet: The Notch signaling pathway was upregulated after hepatocellular carcinoma induction, and the inhibition of Notch signaling suppressed hepatocyte dedifferentiation. ( A ) WISH results showed the expression pattern of notch1a , notch1b , notch2 , notch3 , and her15 at 10 dpf after DOX activation of kras G12V . ( B ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. ( C ) Monolayer images of Anxa4 antibody staining showed expression changes in the DOX-induced kras G12V + and kras G12V + and dnMAML+ groups at 8 dpf and 10 dpf. ( D ) Statistics of Anxa4 + and ZsGreen1 + /ZsGreen1+ ratio in the DOX-induced kras G12V + ( n = 11) and kras G12V + and dnMAML+ ( n = 13) groups at 8 dpf and 10 dpf. ( E ) The results of fluorescent in situ hybridization (FISH) showed sox9b expression in the DOX-induced control and kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. ( F ) The results of FISH showed cp expression in the DOX-induced control, kras G12V + and kras G12V + and dnMAML+ groups at 10 dpf. Numbers indicate the proportion of larvae exhibiting that expression. Asterisks show significance: ****— p < 0.0001. Scale bars—100 μm; error bars—S.D.

    Article Snippet: The cDNA of full-length Zebrafish kras was amplified by PrimeSTAR HS DNA polymerase (Takara) and point mutated to obtain kras G12V , cloned into the modified pTRE3G-BI:ZsGreen1 (Cat.631342, Clontech) vector; and TRE3G-BI is a bidirectional version of TRE3G promoter that allows for simultaneous, equivalent, and inducible expression of two transgenes.

    Techniques: Inhibition, Expressing, Activation Assay, Transgenic Assay, Staining, In Situ Hybridization

    Hepatic Sox9 expression is upregulated after hepatocellular carcinoma induction, and the sox9b fh313 mutant suppressed hepatocyte dedifferentiation. ( A ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. ( B , C ) Sox9 antibody staining indicated the number of Sox9+ cells in the liver at 10 dpf in the DOX-induced control ( n = 5), kras G12V + ( n = 5) and kras G12V + and dnMAML+ ( n = 5) groups with statistical results. ( D ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) treatment strategy in sox9b fh313 mutant. ( E ) Three-dimensional images of Anxa4 antibody staining showed biliary duct changes in the DOX-induced kras G12V + and kras G12V + sox9b fh313 mutants at 8 dpf and 10 dpf. ( F ) Monolayer images of Anxa4 antibody staining biliary duct changes in the DOX-induced kras G12V + and kras G12V + sox9b fh313 mutant at 8 dpf and 10 dpf. Asterisks show significance: *— p < 0.05; **— p < 0.01. Scale bars—100 μm; error bars—S.D.

    Journal: International Journal of Molecular Sciences

    Article Title: Notch–Sox9 Axis Mediates Hepatocyte Dedifferentiation in Kras G12V -Induced Zebrafish Hepatocellular Carcinoma

    doi: 10.3390/ijms23094705

    Figure Lengend Snippet: Hepatic Sox9 expression is upregulated after hepatocellular carcinoma induction, and the sox9b fh313 mutant suppressed hepatocyte dedifferentiation. ( A ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy. ( B , C ) Sox9 antibody staining indicated the number of Sox9+ cells in the liver at 10 dpf in the DOX-induced control ( n = 5), kras G12V + ( n = 5) and kras G12V + and dnMAML+ ( n = 5) groups with statistical results. ( D ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) treatment strategy in sox9b fh313 mutant. ( E ) Three-dimensional images of Anxa4 antibody staining showed biliary duct changes in the DOX-induced kras G12V + and kras G12V + sox9b fh313 mutants at 8 dpf and 10 dpf. ( F ) Monolayer images of Anxa4 antibody staining biliary duct changes in the DOX-induced kras G12V + and kras G12V + sox9b fh313 mutant at 8 dpf and 10 dpf. Asterisks show significance: *— p < 0.05; **— p < 0.01. Scale bars—100 μm; error bars—S.D.

    Article Snippet: The cDNA of full-length Zebrafish kras was amplified by PrimeSTAR HS DNA polymerase (Takara) and point mutated to obtain kras G12V , cloned into the modified pTRE3G-BI:ZsGreen1 (Cat.631342, Clontech) vector; and TRE3G-BI is a bidirectional version of TRE3G promoter that allows for simultaneous, equivalent, and inducible expression of two transgenes.

    Techniques: Expressing, Mutagenesis, Transgenic Assay, Staining

    Inhibition of the Notch signaling pathway after liver cancer induction reduced cancer cell migration and improved survival. ( A ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy to observe the migration of cancer cells. ( B ) Zebrafish larvae were classified into three classes, I, II, and III, by the number of cancer cells outside the liver and the number of locations of metastases. ( C ) The proportion of the total number of larvae in different metastasis classes in the kras G12V + ( n = 46) and kras G12V + and dnMAML+ ( n = 130) groups at 9 dpf. ( D ) Kaplan–Meier survival curves of the DOX-induced kras G12V + ( n = 191) and kras G12V + and dnMAML+ ( n = 163) groups. p values for survival curves were calculated by log-rank test. Scale bars , 100 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Notch–Sox9 Axis Mediates Hepatocyte Dedifferentiation in Kras G12V -Induced Zebrafish Hepatocellular Carcinoma

    doi: 10.3390/ijms23094705

    Figure Lengend Snippet: Inhibition of the Notch signaling pathway after liver cancer induction reduced cancer cell migration and improved survival. ( A ) Tg(fabp10a:Tet3G;TRE3G:kras G12V -ZsGreen1) with Tg(Hsp70l:dnMAML-GFP) double transgenic fish line treatment strategy to observe the migration of cancer cells. ( B ) Zebrafish larvae were classified into three classes, I, II, and III, by the number of cancer cells outside the liver and the number of locations of metastases. ( C ) The proportion of the total number of larvae in different metastasis classes in the kras G12V + ( n = 46) and kras G12V + and dnMAML+ ( n = 130) groups at 9 dpf. ( D ) Kaplan–Meier survival curves of the DOX-induced kras G12V + ( n = 191) and kras G12V + and dnMAML+ ( n = 163) groups. p values for survival curves were calculated by log-rank test. Scale bars , 100 μm.

    Article Snippet: The cDNA of full-length Zebrafish kras was amplified by PrimeSTAR HS DNA polymerase (Takara) and point mutated to obtain kras G12V , cloned into the modified pTRE3G-BI:ZsGreen1 (Cat.631342, Clontech) vector; and TRE3G-BI is a bidirectional version of TRE3G promoter that allows for simultaneous, equivalent, and inducible expression of two transgenes.

    Techniques: Inhibition, Migration, Transgenic Assay