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    TaKaRa tet off advanced gpl cell line
    Characterization of <t>GPL</t> <t>Tet-Off</t> cell lines via luciferase reporter gene transactivation. GPL cells (A to D) or candidate GP Tet-Off cells (E to H) were transfected with a luciferase reporter plasmid under TREtight promoter control (pTREtightLUC). Wells
    Tet Off Advanced Gpl Cell Line, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tet off advanced gpl cell line - by Bioz Stars, 2022-09
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    Characterization of GPL Tet-Off cell lines via luciferase reporter gene transactivation. GPL cells (A to D) or candidate GP Tet-Off cells (E to H) were transfected with a luciferase reporter plasmid under TREtight promoter control (pTREtightLUC). Wells

    Journal: Journal of Virology

    Article Title: A Novel Non-Replication-Competent Cytomegalovirus Capsid Mutant Vaccine Strategy Is Effective in Reducing Congenital Infection

    doi: 10.1128/JVI.00283-16

    Figure Lengend Snippet: Characterization of GPL Tet-Off cell lines via luciferase reporter gene transactivation. GPL cells (A to D) or candidate GP Tet-Off cells (E to H) were transfected with a luciferase reporter plasmid under TREtight promoter control (pTREtightLUC). Wells

    Article Snippet: In order to generate a Tet-Off Advanced GPL cell line, GPL cells in 6-well plates were transfected with pTet-Off Advanced plasmid (4 μg/well; Clontech Laboratories), and cells were maintained under neomycin, G418 (Life Technologies), and antibiotic (400 to 600 μg/ml) selection in complete F-12 medium as described above.

    Techniques: Luciferase, Transfection, Plasmid Preparation

    Growth kinetics of DISC GP85 GPCMV versus wild-type GPCMV on GPL Tet-Off cells. GPL Tet-Off cells were infected at a multiplicity of infection of 1 PFU/cell with each respective virus in separate wells of six-well dishes. Samples were taken on different

    Journal: Journal of Virology

    Article Title: A Novel Non-Replication-Competent Cytomegalovirus Capsid Mutant Vaccine Strategy Is Effective in Reducing Congenital Infection

    doi: 10.1128/JVI.00283-16

    Figure Lengend Snippet: Growth kinetics of DISC GP85 GPCMV versus wild-type GPCMV on GPL Tet-Off cells. GPL Tet-Off cells were infected at a multiplicity of infection of 1 PFU/cell with each respective virus in separate wells of six-well dishes. Samples were taken on different

    Article Snippet: In order to generate a Tet-Off Advanced GPL cell line, GPL cells in 6-well plates were transfected with pTet-Off Advanced plasmid (4 μg/well; Clontech Laboratories), and cells were maintained under neomycin, G418 (Life Technologies), and antibiotic (400 to 600 μg/ml) selection in complete F-12 medium as described above.

    Techniques: Infection

    Regeneration of a DISC GP85 GPCMV from GP85 BAC mutants requires a TRE promoter and a cell line expressing a Tet-Off transactivator (tTA2). (A to C) GP85 mutant GPCMV BACs were individually transfected into GPL cells: GP85/GP86 KmR (A), GP85/86 KmR poly(A)

    Journal: Journal of Virology

    Article Title: A Novel Non-Replication-Competent Cytomegalovirus Capsid Mutant Vaccine Strategy Is Effective in Reducing Congenital Infection

    doi: 10.1128/JVI.00283-16

    Figure Lengend Snippet: Regeneration of a DISC GP85 GPCMV from GP85 BAC mutants requires a TRE promoter and a cell line expressing a Tet-Off transactivator (tTA2). (A to C) GP85 mutant GPCMV BACs were individually transfected into GPL cells: GP85/GP86 KmR (A), GP85/86 KmR poly(A)

    Article Snippet: In order to generate a Tet-Off Advanced GPL cell line, GPL cells in 6-well plates were transfected with pTet-Off Advanced plasmid (4 μg/well; Clontech Laboratories), and cells were maintained under neomycin, G418 (Life Technologies), and antibiotic (400 to 600 μg/ml) selection in complete F-12 medium as described above.

    Techniques: BAC Assay, Expressing, Mutagenesis, Transfection