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  • 93
    ATCC plasmid dna template phpv 16
    Plasmid Dna Template Phpv 16, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna template phpv 16/product/ATCC
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    plasmid dna template phpv 16 - by Bioz Stars, 2020-10
    93/100 stars
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    99
    Millipore plasmid dna template
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Plasmid Dna Template, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna template/product/Millipore
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    plasmid dna template - by Bioz Stars, 2020-10
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    93
    GenScript dna template
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Dna Template, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 120 article reviews
    Price from $9.99 to $1999.99
    dna template - by Bioz Stars, 2020-10
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    93
    Thermo Fisher ampflstr kit template 7 cd
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Ampflstr Kit Template 7 Cd, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampflstr kit template 7 cd/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ampflstr kit template 7 cd - by Bioz Stars, 2020-10
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    99
    Millipore plasmid dna template psgat
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Plasmid Dna Template Psgat, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna template psgat/product/Millipore
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    plasmid dna template psgat - by Bioz Stars, 2020-10
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    90
    Goodfellow Cambridge plasmid dna template
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Plasmid Dna Template, supplied by Goodfellow Cambridge, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 2 article reviews
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    plasmid dna template - by Bioz Stars, 2020-10
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    91
    Macrogen plasmid dna templates
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Plasmid Dna Templates, supplied by Macrogen, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 3 article reviews
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    plasmid dna templates - by Bioz Stars, 2020-10
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    89
    Thermo Fisher plasmid dna templates
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Plasmid Dna Templates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 146 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 146 article reviews
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    plasmid dna templates - by Bioz Stars, 2020-10
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    93
    Addgene inc dna plasmid templates
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Dna Plasmid Templates, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 7 article reviews
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    dna plasmid templates - by Bioz Stars, 2020-10
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    99
    Addgene inc single stranded dna oligonucleotide template crispr cas plasmids pspcas9 bb 2 a gfp px458
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Single Stranded Dna Oligonucleotide Template Crispr Cas Plasmids Pspcas9 Bb 2 A Gfp Px458, supplied by Addgene inc, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single stranded dna oligonucleotide template crispr cas plasmids pspcas9 bb 2 a gfp px458/product/Addgene inc
    Average 99 stars, based on 8 article reviews
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    single stranded dna oligonucleotide template crispr cas plasmids pspcas9 bb 2 a gfp px458 - by Bioz Stars, 2020-10
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    89
    ATUM template plasmid dna
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Template Plasmid Dna, supplied by ATUM, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/template plasmid dna/product/ATUM
    Average 89 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    template plasmid dna - by Bioz Stars, 2020-10
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    99
    Millipore template plasmid dna
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Template Plasmid Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/template plasmid dna/product/Millipore
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    template plasmid dna - by Bioz Stars, 2020-10
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    90
    OriGene template plasmid dna
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Template Plasmid Dna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/template plasmid dna/product/OriGene
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    template plasmid dna - by Bioz Stars, 2020-10
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    91
    Source BioScience plc template plasmid dna
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Template Plasmid Dna, supplied by Source BioScience plc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/template plasmid dna/product/Source BioScience plc
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    template plasmid dna - by Bioz Stars, 2020-10
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    89
    Thermo Fisher template plasmid dna
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Template Plasmid Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/template plasmid dna/product/Thermo Fisher
    Average 89 stars, based on 77 article reviews
    Price from $9.99 to $1999.99
    template plasmid dna - by Bioz Stars, 2020-10
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    91
    Addgene inc template dna plasmid pegfp
    Co-IP of p12 with the viral genomic <t>DNA</t> and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome <t>(‘pNCS’)</t> were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.
    Template Dna Plasmid Pegfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/template dna plasmid pegfp/product/Addgene inc
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    template dna plasmid pegfp - by Bioz Stars, 2020-10
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    Co-IP of p12 with the viral genomic DNA and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome (‘pNCS’) were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.

    Journal: PLoS Pathogens

    Article Title: The Gag Cleavage Product, p12, is a Functional Constituent of the Murine Leukemia Virus Pre-Integration Complex

    doi: 10.1371/journal.ppat.1001183

    Figure Lengend Snippet: Co-IP of p12 with the viral genomic DNA and CA proteins. Lysates were prepared from NIH3T3 cells, infected with wt or 1xMycR viruses. To detect the viral genomic DNA (A and B) the lysates were incubated with anti-Myc (labeled ‘αMyc’), or control anti-Flag antibodies (labeled ‘αFlag’); both conjugated to protein G magnetic beads. Viral genomic DNA was PCR amplified from cell lysates (labeled ‘Input’) and from the magnetic beads (labeled ‘IP’) with MLV specific primers. After 25 and 30 cycles of amplification, the PCR products (875 bp long) were electrophoresed in 1% agarose gels containing ethidium bromide (A). ‘Mock’ indicates mock-infected NIH3T3 cells. Water and plasmid encoding the MLV genome (‘pNCS’) were used as negative and positive PCR controls, respectively. (B) The ‘Relative IP efficiency’ of the viral genomic DNA was quantified by qPCR ( Materials and Methods ) and is presented as the means ± the standard error of the means, obtained from three independent experiments. The qPCR values are presented in Fig. S7A . To test for CA immunoprecipitation (C), NIH3T3 were infected as in (A) and 15% of the indicated cell lysate was used to determine total CA levels (labeled ‘Cell Lysates’). The remaining lysates (labeled as in A) were used for IP with anti-Myc, or control anti-Flag monoclonal antibodies, bound to agarose beads. Cells exposed to medium with no virus served as a mock control. Pellets (labeled ‘IP’) and cell lysates were analyzed by Western Blot, using anti-CA polyclonal antibodies.

    Article Snippet: The biotin-labeled probe was prepared in a nick-translation reaction (100 µl, 2 h at 16°C), containing nick-translation buffer (50 mM Tris-HCl pH 7.8, 5 mM MgCl2 ,50 ng/ml BSA), plasmid DNA template (pNCS, 2 µg), dATP, dGTP, dCTP (50 nM of each nucleotide, Sigma), and biotin-11-dUTP (50 nM, Roche), β-mercaptoethanol (10 mM), DNaseI (30 ng/ml, freshly diluted), Klenow polymerase (20 U, New England Biolabs).

    Techniques: Co-Immunoprecipitation Assay, Infection, Incubation, Labeling, Magnetic Beads, Polymerase Chain Reaction, Amplification, Plasmid Preparation, Real-time Polymerase Chain Reaction, Immunoprecipitation, Western Blot