template dna Takara Search Results


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  • 94
    TaKaRa complementary dna template generated by the reverse transcription kit takara
    Complementary Dna Template Generated By The Reverse Transcription Kit Takara, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    TaKaRa pcr template preparation kit
    Pcr Template Preparation Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    TaKaRa template dna
    Inverse <t>PCR</t> (iPCR) and sequencing of the boundary region surrounding the Mk + 1.3 locus. ( a ) Agarose gel electrophoresis of nested PCR products stained with ethidium bromide. The gel is the full length gel and lanes have not been cropped or stitched. ( b ) Chromatograms generated from direct sequencing of the iPCR products. Black and red letters above the chromatograms indicate the sequences of chromosomal positions chr 7: 62,416,891–62,416,891 and chr 7: 62,268,615–62,268,638 (on the University of California, Santa Cruz, genome browser (GRCm38/mm10)), respectively. W: wild-type <t>DNA,</t> Tg: hemizygous transgenic DNA, M: molecular size marker.
    Template Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 5922 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    TaKaRa dna fragment
    Inverse <t>PCR</t> (iPCR) and sequencing of the boundary region surrounding the Mk + 1.3 locus. ( a ) Agarose gel electrophoresis of nested PCR products stained with ethidium bromide. The gel is the full length gel and lanes have not been cropped or stitched. ( b ) Chromatograms generated from direct sequencing of the iPCR products. Black and red letters above the chromatograms indicate the sequences of chromosomal positions chr 7: 62,416,891–62,416,891 and chr 7: 62,268,615–62,268,638 (on the University of California, Santa Cruz, genome browser (GRCm38/mm10)), respectively. W: wild-type <t>DNA,</t> Tg: hemizygous transgenic DNA, M: molecular size marker.
    Dna Fragment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 4673 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa puc18 template dna
    Representative ultraviolet images from ethidium bromide-stained PAGE gels of the 110 nt PCR product derived from <t>pUC18</t> (amplifying region I) and the C5-modified nucleoside triphosphates. ( A–C ) The reaction mixtures containing dATP, dGTP, dCTP and T AL (lane 3), T AC (lane 4), T AF (lane 5), T PA (lane 6), T PN (lane 7), T PR (lane 8), T A2 (lane 11), T A4 (lane 12), T A6 (lane 13), T G6 (lane 14), T ME (lane 15), T CN (lane 16), or T DH (lane 17) were separated by electrophoresis on denaturing PAGE. Except for the positive control, the reaction mixtures do not contain natural TTP. The PCR product was generated by the positive control (reaction mixture containing dATP, dGTP, dCTP and TTP; lanes 2 and 10) but not by the negative control (reaction mixture containing dATP, dGTP and dCTP; lanes 1 and 9). ( D–F ) Reaction mixtures containing dATP, dGTP, TTP and C AL (lane 3), C AC (lane 4), C AF (lane 5), C PA (lane 6), C PN (lane 7), C PR (lane 8), C A2 (lane 11), C A4 (lane 12), C A6 (lane 13), C G6 (lane 14), C ME (lane 15), C CN (lane 16) or C DH (lane 17) were separated by electrophoresis on denaturing PAGE. Except for the positive control, the reaction mixtures did not contain natural dCTP. The PCR product was generated by the positive control (reaction mixture containing dATP, dGTP, dCTP and TTP; lanes 2 and 10) but not by the negative control (reaction mixture containing dATP, dGTP and TTP; lanes 1 and 9). The thermostable <t>DNA</t> polymerases used were Taq (A, D), Vent(exo-) (B and E), and KOD Dash (C and F).
    Puc18 Template Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa dna templates
    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using <t>PCR</t> with the primers having a Taq I site at the end. After cut with Taq I , the same amount of <t>DNA</t> for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
    Dna Templates, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 384 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    TaKaRa λ control template dna fragment
    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using <t>PCR</t> with the primers having a Taq I site at the end. After cut with Taq I , the same amount of <t>DNA</t> for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
    λ Control Template Dna Fragment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    TaKaRa circular template dna
    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using <t>PCR</t> with the primers having a Taq I site at the end. After cut with Taq I , the same amount of <t>DNA</t> for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
    Circular Template Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    TaKaRa dna polymerization
    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using <t>PCR</t> with the primers having a Taq I site at the end. After cut with Taq I , the same amount of <t>DNA</t> for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
    Dna Polymerization, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa lambda control template dna fragment
    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using <t>PCR</t> with the primers having a Taq I site at the end. After cut with Taq I , the same amount of <t>DNA</t> for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
    Lambda Control Template Dna Fragment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    TaKaRa 250 ng template dna
    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using <t>PCR</t> with the primers having a Taq I site at the end. After cut with Taq I , the same amount of <t>DNA</t> for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
    250 Ng Template Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    TaKaRa 100 ng template dna
    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using <t>PCR</t> with the primers having a Taq I site at the end. After cut with Taq I , the same amount of <t>DNA</t> for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
    100 Ng Template Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    TaKaRa bisulfite treated dna
    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using <t>PCR</t> with the primers having a Taq I site at the end. After cut with Taq I , the same amount of <t>DNA</t> for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
    Bisulfite Treated Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 547 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa green fluorescence protein gfp dna dna
    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using <t>PCR</t> with the primers having a Taq I site at the end. After cut with Taq I , the same amount of <t>DNA</t> for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
    Green Fluorescence Protein Gfp Dna Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa random primer dna labelling kit
    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using <t>PCR</t> with the primers having a Taq I site at the end. After cut with Taq I , the same amount of <t>DNA</t> for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
    Random Primer Dna Labelling Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    takara dna template prep kit 2 0
    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using <t>PCR</t> with the primers having a Taq I site at the end. After cut with Taq I , the same amount of <t>DNA</t> for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
    Dna Template Prep Kit 2 0, supplied by takara, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    TaKaRa assay template human reference dna
    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using <t>PCR</t> with the primers having a Taq I site at the end. After cut with Taq I , the same amount of <t>DNA</t> for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
    Assay Template Human Reference Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa dna templates e coli strain dh5α
    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using <t>PCR</t> with the primers having a Taq I site at the end. After cut with Taq I , the same amount of <t>DNA</t> for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
    Dna Templates E Coli Strain Dh5α, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa dna polymerase
    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using <t>PCR</t> with the primers having a Taq I site at the end. After cut with Taq I , the same amount of <t>DNA</t> for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
    Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Inverse PCR (iPCR) and sequencing of the boundary region surrounding the Mk + 1.3 locus. ( a ) Agarose gel electrophoresis of nested PCR products stained with ethidium bromide. The gel is the full length gel and lanes have not been cropped or stitched. ( b ) Chromatograms generated from direct sequencing of the iPCR products. Black and red letters above the chromatograms indicate the sequences of chromosomal positions chr 7: 62,416,891–62,416,891 and chr 7: 62,268,615–62,268,638 (on the University of California, Santa Cruz, genome browser (GRCm38/mm10)), respectively. W: wild-type DNA, Tg: hemizygous transgenic DNA, M: molecular size marker.

    Journal: Scientific Reports

    Article Title: Application of droplet digital PCR in the analysis of genome integration and organization of the transgene in BAC transgenic mice

    doi: 10.1038/s41598-018-25001-x

    Figure Lengend Snippet: Inverse PCR (iPCR) and sequencing of the boundary region surrounding the Mk + 1.3 locus. ( a ) Agarose gel electrophoresis of nested PCR products stained with ethidium bromide. The gel is the full length gel and lanes have not been cropped or stitched. ( b ) Chromatograms generated from direct sequencing of the iPCR products. Black and red letters above the chromatograms indicate the sequences of chromosomal positions chr 7: 62,416,891–62,416,891 and chr 7: 62,268,615–62,268,638 (on the University of California, Santa Cruz, genome browser (GRCm38/mm10)), respectively. W: wild-type DNA, Tg: hemizygous transgenic DNA, M: molecular size marker.

    Article Snippet: Nested amplification around the circular DNA using primers oriented in a direction opposite to that of conventional PCR was performed using reaction mixtures containing DNA template, 1 × Ex Taq buffer (containing 2.0 mM of MgCl2 ), 250 μM of dNTP mix, 0.5 μM of each primer, and 0.25 U/μl of Ex Taq DNA Polymerase (Takara Bio, Otsu, Japan).

    Techniques: Inverse PCR, Sequencing, Agarose Gel Electrophoresis, Nested PCR, Staining, Generated, Transgenic Assay, Marker

    Tumor-promoting SV40 ST mimics Tws function to stabilize Plk4 and amplify centrioles. (A) S2 cells overexpressing GFP or ST-GFP (green) immunostained for PLP (red) to mark centrioles. DNA (blue) is Hoechst stained. (B) ST-GFP overexpression increases centriole numbers but not in the presence of OA. ST-GFP expression also rescues centriole loss caused by depletion of Tws but not Plk4. Centrioles were counted after 6-d RNAi or 24-h OA treatment in cells transfected with inducible GFP or ST-GFP (expression was induced during the last 2 d). Mean values (numbers) of two experiments are shown ( n = 600 cells/treatment). Asterisks indicate significant differences (P

    Journal: The Journal of Cell Biology

    Article Title: The Protein Phosphatase 2A regulatory subunit Twins stabilizes Plk4 to induce centriole amplification

    doi: 10.1083/jcb.201107086

    Figure Lengend Snippet: Tumor-promoting SV40 ST mimics Tws function to stabilize Plk4 and amplify centrioles. (A) S2 cells overexpressing GFP or ST-GFP (green) immunostained for PLP (red) to mark centrioles. DNA (blue) is Hoechst stained. (B) ST-GFP overexpression increases centriole numbers but not in the presence of OA. ST-GFP expression also rescues centriole loss caused by depletion of Tws but not Plk4. Centrioles were counted after 6-d RNAi or 24-h OA treatment in cells transfected with inducible GFP or ST-GFP (expression was induced during the last 2 d). Mean values (numbers) of two experiments are shown ( n = 600 cells/treatment). Asterisks indicate significant differences (P

    Article Snippet: Control dsRNA was synthesized from control DNA template amplified from a non-GFP sequence of the pEGFP-N1 vector (Takara Bio Inc.) using the primers 5′-CGCTTTTCTGGATTCATCGAC-3′ and 5′-TGAGTAACCTGAGGCTATGG-3′ (all primers used for dsRNA synthesis in this study begin with the T7 promotes sequence 5′-TAATACGACTCACTATAGGG-3′).

    Techniques: Plasmid Purification, Staining, Over Expression, Expressing, Transfection

    Representative ultraviolet images from ethidium bromide-stained PAGE gels of the 110 nt PCR product derived from pUC18 (amplifying region I) and the C5-modified nucleoside triphosphates. ( A–C ) The reaction mixtures containing dATP, dGTP, dCTP and T AL (lane 3), T AC (lane 4), T AF (lane 5), T PA (lane 6), T PN (lane 7), T PR (lane 8), T A2 (lane 11), T A4 (lane 12), T A6 (lane 13), T G6 (lane 14), T ME (lane 15), T CN (lane 16), or T DH (lane 17) were separated by electrophoresis on denaturing PAGE. Except for the positive control, the reaction mixtures do not contain natural TTP. The PCR product was generated by the positive control (reaction mixture containing dATP, dGTP, dCTP and TTP; lanes 2 and 10) but not by the negative control (reaction mixture containing dATP, dGTP and dCTP; lanes 1 and 9). ( D–F ) Reaction mixtures containing dATP, dGTP, TTP and C AL (lane 3), C AC (lane 4), C AF (lane 5), C PA (lane 6), C PN (lane 7), C PR (lane 8), C A2 (lane 11), C A4 (lane 12), C A6 (lane 13), C G6 (lane 14), C ME (lane 15), C CN (lane 16) or C DH (lane 17) were separated by electrophoresis on denaturing PAGE. Except for the positive control, the reaction mixtures did not contain natural dCTP. The PCR product was generated by the positive control (reaction mixture containing dATP, dGTP, dCTP and TTP; lanes 2 and 10) but not by the negative control (reaction mixture containing dATP, dGTP and TTP; lanes 1 and 9). The thermostable DNA polymerases used were Taq (A, D), Vent(exo-) (B and E), and KOD Dash (C and F).

    Journal: Nucleic Acids Research

    Article Title: Systematic characterization of 2?-deoxynucleoside- 5?-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA

    doi: 10.1093/nar/gkl637

    Figure Lengend Snippet: Representative ultraviolet images from ethidium bromide-stained PAGE gels of the 110 nt PCR product derived from pUC18 (amplifying region I) and the C5-modified nucleoside triphosphates. ( A–C ) The reaction mixtures containing dATP, dGTP, dCTP and T AL (lane 3), T AC (lane 4), T AF (lane 5), T PA (lane 6), T PN (lane 7), T PR (lane 8), T A2 (lane 11), T A4 (lane 12), T A6 (lane 13), T G6 (lane 14), T ME (lane 15), T CN (lane 16), or T DH (lane 17) were separated by electrophoresis on denaturing PAGE. Except for the positive control, the reaction mixtures do not contain natural TTP. The PCR product was generated by the positive control (reaction mixture containing dATP, dGTP, dCTP and TTP; lanes 2 and 10) but not by the negative control (reaction mixture containing dATP, dGTP and dCTP; lanes 1 and 9). ( D–F ) Reaction mixtures containing dATP, dGTP, TTP and C AL (lane 3), C AC (lane 4), C AF (lane 5), C PA (lane 6), C PN (lane 7), C PR (lane 8), C A2 (lane 11), C A4 (lane 12), C A6 (lane 13), C G6 (lane 14), C ME (lane 15), C CN (lane 16) or C DH (lane 17) were separated by electrophoresis on denaturing PAGE. Except for the positive control, the reaction mixtures did not contain natural dCTP. The PCR product was generated by the positive control (reaction mixture containing dATP, dGTP, dCTP and TTP; lanes 2 and 10) but not by the negative control (reaction mixture containing dATP, dGTP and TTP; lanes 1 and 9). The thermostable DNA polymerases used were Taq (A, D), Vent(exo-) (B and E), and KOD Dash (C and F).

    Article Snippet: Primers 1F, 1R, 2F and 2R were purchased from JBioS, and pUC18 template DNA was obtained from TaKaRa Biomedicals.

    Techniques: Staining, Polyacrylamide Gel Electrophoresis, Polymerase Chain Reaction, Derivative Assay, Modification, Electrophoresis, Positive Control, Generated, Negative Control

    Sequences of amplifying regions of the pUC18 template DNA. Primer sequences are underlined.

    Journal: Nucleic Acids Research

    Article Title: Systematic characterization of 2?-deoxynucleoside- 5?-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA

    doi: 10.1093/nar/gkl637

    Figure Lengend Snippet: Sequences of amplifying regions of the pUC18 template DNA. Primer sequences are underlined.

    Article Snippet: Primers 1F, 1R, 2F and 2R were purchased from JBioS, and pUC18 template DNA was obtained from TaKaRa Biomedicals.

    Techniques:

    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using PCR with the primers having a Taq I site at the end. After cut with Taq I , the same amount of DNA for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.

    Journal: BMC Genomics

    Article Title: Sensitivity and fidelity of DNA microarray improved with integration of Amplified Differential Gene Expression (ADGE)

    doi: 10.1186/1471-2164-4-28

    Figure Lengend Snippet: Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using PCR with the primers having a Taq I site at the end. After cut with Taq I , the same amount of DNA for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.

    Article Snippet: The PCR labeling reaction was set up with 42 μl of DNA templates, 5 μl of 10x Clontech PCR buffer, 1 μl of 10 mM dNTPS containing 8 mM aminoallyl-dUTP and 2 mM dTTP, 1 μl of 10 μM CT primer or TT primer and 1 μl of Clontech cDNA polymerase.

    Techniques: Microarray, Clone Assay, Amplification, Polymerase Chain Reaction