template dna Takara Search Results


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  • 96
    TaKaRa template dna
    Template Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 4077 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 4077 article reviews
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    template dna - by Bioz Stars, 2020-01
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    85
    TaKaRa random primer dna labelling kit
    Random Primer Dna Labelling Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 36 article reviews
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    95
    TaKaRa human genomic dna
    Human Genomic Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human genomic dna - by Bioz Stars, 2020-01
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    96
    TaKaRa dna polymerase
    Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1742 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1742 article reviews
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    95
    TaKaRa t4 dna polymerase
    T4 Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 677 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa primestar gxl dna polymerase
    Primestar Gxl Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa primestar max dna polymerase
    Primestar Max Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2987 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa titanium taq dna polymerase
    Titanium Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa primestar hs dna polymerase
    Primestar Hs Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 5762 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    TaKaRa taq dna polymerase
    Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 9499 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 98 stars, based on 9499 article reviews
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    99
    TaKaRa extaq dna polymerase
    Extaq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 3129 article reviews
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    extaq dna polymerase - by Bioz Stars, 2020-01
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    99
    TaKaRa la taq dna polymerase
    La Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3376 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa rtaq dna polymerase
    Rtaq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1416 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    TaKaRa high fidelity pfu dna polymerase
    High Fidelity Pfu Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    TaKaRa p labeled dna probes
    AGO4-mediated RdDM plays a role in defense against <t>CLCuMuV</t> infection. (A) Viral symptoms in NbAGO4 -silenced plants infected with CLCuMuV at 18 dpi. (B) Southern blot detection showing viral <t>DNA</t> accumulation in NbAGO4 -silenced and control plants. A partial CLCuMuV V1 DNA was used as a template to make a 32 P-labeled probe. Total DNA was stained with ethidium bromide as a loading control. Single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) corresponding to CLCuMuV are indicated by arrows. (C) Detection of cytosine methylation status of the CLCuMuV 5′ intergenic region (IR) in NbAGO4 -silenced or control plants. The histogram represents the proportions of methylated cytosine in different sequence groups. The asterisk indicates significant differences (*, P
    P Labeled Dna Probes, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    TaKaRa high fidelity pyrobest dna polymerase
    AGO4-mediated RdDM plays a role in defense against <t>CLCuMuV</t> infection. (A) Viral symptoms in NbAGO4 -silenced plants infected with CLCuMuV at 18 dpi. (B) Southern blot detection showing viral <t>DNA</t> accumulation in NbAGO4 -silenced and control plants. A partial CLCuMuV V1 DNA was used as a template to make a 32 P-labeled probe. Total DNA was stained with ethidium bromide as a loading control. Single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) corresponding to CLCuMuV are indicated by arrows. (C) Detection of cytosine methylation status of the CLCuMuV 5′ intergenic region (IR) in NbAGO4 -silenced or control plants. The histogram represents the proportions of methylated cytosine in different sequence groups. The asterisk indicates significant differences (*, P
    High Fidelity Pyrobest Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    TaKaRa polymerase
    AGO4-mediated RdDM plays a role in defense against <t>CLCuMuV</t> infection. (A) Viral symptoms in NbAGO4 -silenced plants infected with CLCuMuV at 18 dpi. (B) Southern blot detection showing viral <t>DNA</t> accumulation in NbAGO4 -silenced and control plants. A partial CLCuMuV V1 DNA was used as a template to make a 32 P-labeled probe. Total DNA was stained with ethidium bromide as a loading control. Single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) corresponding to CLCuMuV are indicated by arrows. (C) Detection of cytosine methylation status of the CLCuMuV 5′ intergenic region (IR) in NbAGO4 -silenced or control plants. The histogram represents the proportions of methylated cytosine in different sequence groups. The asterisk indicates significant differences (*, P
    Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 206 article reviews
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    97
    TaKaRa cdna template
    AGO4-mediated RdDM plays a role in defense against <t>CLCuMuV</t> infection. (A) Viral symptoms in NbAGO4 -silenced plants infected with CLCuMuV at 18 dpi. (B) Southern blot detection showing viral <t>DNA</t> accumulation in NbAGO4 -silenced and control plants. A partial CLCuMuV V1 DNA was used as a template to make a 32 P-labeled probe. Total DNA was stained with ethidium bromide as a loading control. Single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) corresponding to CLCuMuV are indicated by arrows. (C) Detection of cytosine methylation status of the CLCuMuV 5′ intergenic region (IR) in NbAGO4 -silenced or control plants. The histogram represents the proportions of methylated cytosine in different sequence groups. The asterisk indicates significant differences (*, P
    Cdna Template, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 2543 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa ex taq hot start version
    AGO4-mediated RdDM plays a role in defense against <t>CLCuMuV</t> infection. (A) Viral symptoms in NbAGO4 -silenced plants infected with CLCuMuV at 18 dpi. (B) Southern blot detection showing viral <t>DNA</t> accumulation in NbAGO4 -silenced and control plants. A partial CLCuMuV V1 DNA was used as a template to make a 32 P-labeled probe. Total DNA was stained with ethidium bromide as a loading control. Single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) corresponding to CLCuMuV are indicated by arrows. (C) Detection of cytosine methylation status of the CLCuMuV 5′ intergenic region (IR) in NbAGO4 -silenced or control plants. The histogram represents the proportions of methylated cytosine in different sequence groups. The asterisk indicates significant differences (*, P
    Ex Taq Hot Start Version, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 900 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa la taq enzyme
    AGO4-mediated RdDM plays a role in defense against <t>CLCuMuV</t> infection. (A) Viral symptoms in NbAGO4 -silenced plants infected with CLCuMuV at 18 dpi. (B) Southern blot detection showing viral <t>DNA</t> accumulation in NbAGO4 -silenced and control plants. A partial CLCuMuV V1 DNA was used as a template to make a 32 P-labeled probe. Total DNA was stained with ethidium bromide as a loading control. Single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) corresponding to CLCuMuV are indicated by arrows. (C) Detection of cytosine methylation status of the CLCuMuV 5′ intergenic region (IR) in NbAGO4 -silenced or control plants. The histogram represents the proportions of methylated cytosine in different sequence groups. The asterisk indicates significant differences (*, P
    La Taq Enzyme, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 173 article reviews
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    la taq enzyme - by Bioz Stars, 2020-01
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    94
    TaKaRa 1st strand cdna synthesis kit
    AGO4-mediated RdDM plays a role in defense against <t>CLCuMuV</t> infection. (A) Viral symptoms in NbAGO4 -silenced plants infected with CLCuMuV at 18 dpi. (B) Southern blot detection showing viral <t>DNA</t> accumulation in NbAGO4 -silenced and control plants. A partial CLCuMuV V1 DNA was used as a template to make a 32 P-labeled probe. Total DNA was stained with ethidium bromide as a loading control. Single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) corresponding to CLCuMuV are indicated by arrows. (C) Detection of cytosine methylation status of the CLCuMuV 5′ intergenic region (IR) in NbAGO4 -silenced or control plants. The histogram represents the proportions of methylated cytosine in different sequence groups. The asterisk indicates significant differences (*, P
    1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa advantage hd polymerase
    AGO4-mediated RdDM plays a role in defense against <t>CLCuMuV</t> infection. (A) Viral symptoms in NbAGO4 -silenced plants infected with CLCuMuV at 18 dpi. (B) Southern blot detection showing viral <t>DNA</t> accumulation in NbAGO4 -silenced and control plants. A partial CLCuMuV V1 DNA was used as a template to make a 32 P-labeled probe. Total DNA was stained with ethidium bromide as a loading control. Single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) corresponding to CLCuMuV are indicated by arrows. (C) Detection of cytosine methylation status of the CLCuMuV 5′ intergenic region (IR) in NbAGO4 -silenced or control plants. The histogram represents the proportions of methylated cytosine in different sequence groups. The asterisk indicates significant differences (*, P
    Advantage Hd Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    TaKaRa klenow fragment
    AGO4-mediated RdDM plays a role in defense against <t>CLCuMuV</t> infection. (A) Viral symptoms in NbAGO4 -silenced plants infected with CLCuMuV at 18 dpi. (B) Southern blot detection showing viral <t>DNA</t> accumulation in NbAGO4 -silenced and control plants. A partial CLCuMuV V1 DNA was used as a template to make a 32 P-labeled probe. Total DNA was stained with ethidium bromide as a loading control. Single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) corresponding to CLCuMuV are indicated by arrows. (C) Detection of cytosine methylation status of the CLCuMuV 5′ intergenic region (IR) in NbAGO4 -silenced or control plants. The histogram represents the proportions of methylated cytosine in different sequence groups. The asterisk indicates significant differences (*, P
    Klenow Fragment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 563 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa snx2 cdna
    Construction and characterization of SNX1, SNX1A, <t>SNX2,</t> SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length <t>cDNA,</t> the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.
    Snx2 Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa primescript reverse transcriptase
    Construction and characterization of SNX1, SNX1A, <t>SNX2,</t> SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length <t>cDNA,</t> the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.
    Primescript Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3402 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    TaKaRa m mlv reverse transcriptase
    Construction and characterization of SNX1, SNX1A, <t>SNX2,</t> SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length <t>cDNA,</t> the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.
    M Mlv Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 2983 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa dntp mixture
    Construction and characterization of SNX1, SNX1A, <t>SNX2,</t> SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length <t>cDNA,</t> the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.
    Dntp Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3226 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa marathon cdna amplification kit
    Construction and characterization of SNX1, SNX1A, <t>SNX2,</t> SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length <t>cDNA,</t> the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.
    Marathon Cdna Amplification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    TaKaRa smarttm pcr cdna synthesis kit
    Construction and characterization of SNX1, SNX1A, <t>SNX2,</t> SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length <t>cDNA,</t> the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.
    Smarttm Pcr Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    70
    TaKaRa lm pcr apoalert lm pcr ladder assay kit
    Construction and characterization of SNX1, SNX1A, <t>SNX2,</t> SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length <t>cDNA,</t> the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.
    Lm Pcr Apoalert Lm Pcr Ladder Assay Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa race ready heart cdna template
    Construction and characterization of SNX1, SNX1A, <t>SNX2,</t> SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length <t>cDNA,</t> the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.
    Race Ready Heart Cdna Template, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    race ready heart cdna template - by Bioz Stars, 2020-01
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    76
    TaKaRa mouse cdna template
    Construction and characterization of SNX1, SNX1A, <t>SNX2,</t> SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length <t>cDNA,</t> the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.
    Mouse Cdna Template, supplied by TaKaRa, used in various techniques. Bioz Stars score: 76/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse cdna template - by Bioz Stars, 2020-01
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    AGO4-mediated RdDM plays a role in defense against CLCuMuV infection. (A) Viral symptoms in NbAGO4 -silenced plants infected with CLCuMuV at 18 dpi. (B) Southern blot detection showing viral DNA accumulation in NbAGO4 -silenced and control plants. A partial CLCuMuV V1 DNA was used as a template to make a 32 P-labeled probe. Total DNA was stained with ethidium bromide as a loading control. Single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) corresponding to CLCuMuV are indicated by arrows. (C) Detection of cytosine methylation status of the CLCuMuV 5′ intergenic region (IR) in NbAGO4 -silenced or control plants. The histogram represents the proportions of methylated cytosine in different sequence groups. The asterisk indicates significant differences (*, P

    Journal: Journal of Virology

    Article Title: Geminiviral V2 Protein Suppresses Transcriptional Gene Silencing through Interaction with AGO4

    doi: 10.1128/JVI.01675-18

    Figure Lengend Snippet: AGO4-mediated RdDM plays a role in defense against CLCuMuV infection. (A) Viral symptoms in NbAGO4 -silenced plants infected with CLCuMuV at 18 dpi. (B) Southern blot detection showing viral DNA accumulation in NbAGO4 -silenced and control plants. A partial CLCuMuV V1 DNA was used as a template to make a 32 P-labeled probe. Total DNA was stained with ethidium bromide as a loading control. Single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) corresponding to CLCuMuV are indicated by arrows. (C) Detection of cytosine methylation status of the CLCuMuV 5′ intergenic region (IR) in NbAGO4 -silenced or control plants. The histogram represents the proportions of methylated cytosine in different sequence groups. The asterisk indicates significant differences (*, P

    Article Snippet: Partial CLCuMuV V1 DNA was used as the template to generate 32 P-labeled DNA probes (TaKaRa, Japan).

    Techniques: Infection, Southern Blot, Labeling, Staining, Methylation, Sequencing

    Construction and characterization of SNX1, SNX1A, SNX2, SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length cDNA, the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.

    Journal: Molecular and Cellular Biology

    Article Title: Identification of a Family of Sorting Nexin Molecules and Characterization of Their Association with Receptors

    doi:

    Figure Lengend Snippet: Construction and characterization of SNX1, SNX1A, SNX2, SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length cDNA, the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.

    Article Snippet: A 5′-rapid amplification of cDNA ends (RACE) strategy (Life Technologies, Inc., Gaithersburg, Md.) with total human skeletal muscle as a template was used to complete the 5′ end of the SNX2 cDNA (Clontech).

    Techniques: Polymerase Chain Reaction, Construct